pycom16g11240

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006515
Physical Location & Seq
Reverse (-)
7794419 .. 7795300
882 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 882 bp
ATGATGTTTCCGCATTGTTCCATGACCTTGACAATGGCATGCTTCCTATCAGTGTCATCTTCCCATACCTTCCCATCCCAGCTCACCGTCGCCGTGACCAGGCCCGAAAGAAGCTTTCAGATATCTTTGCAAAGATCGATCCGAAATTCCCGTAAGCGTGCTGCACAGTCTGAGAAGGACATGTTGCAATGCTTCATTGACTCAAAGTACAAAAATGGCCGACCAACAACTGAATCTGAGGTCACTGGCTTGCTCATTGCTGCTCTCTTTGCTGGGCAACACACTAGCTCTATCACTTCCACTTGGACTGGGGCCTATCTCCTCCGTCACAAGCAGTTCTTATCTGCTGTGTCAGAGGAGCAGAAAAACATAATGAAAAAGCATGGAAAGAAGATTGATCATGATATACTGGCAGAGATGGATGTCCTGTATCGTTGCATCAAAGAAGCTCTCAGATTGCACCCTCCACTGATCATTCTGCTGCGAAGCTCGCATAGTGATTTCAGCGTAAAAACCAACGAGGGGAAGGAGTATGATATCCCGAAGGGGCACATAGTAGCCGCGTCACCATCTTTTGCCAACCGGCTTCCTCATATTTACAAGGAGCCAGACAGCTACGATCCTGATAGATTTGCTGCTGGGAGAGAAGAAGACAAGTCAGCAGGGGCATTCTCATACATCTCGTTTGGAGGTGGCAGGCATGGATGCCTTGGTGAGCCTTTTGCATACCTACAGATAAAGGCTATATGGAGCCACCTGCTGAGAAATTTTGAACTGGAGCTGGTATCACCATTTCCCAAGACGGATTGGAATGCCATGGTTGTGGGTGTGAAAGGAAAGGTGATGGTGCGGTACAAGCGTCGGGAGCTTTCAGTTGAATAA

Protein Analysis

294

Amino Acids

33.14

Weight (kDa)

9.35

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 48 - 269 1.4e-46 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 765
Acc36I ACCTGC 1 cut(s) 765
AccII CGCG 1 cut(s) 563
AciI CCGC 3 cut(s) 11, 561, 850
AclWI GGATC 2 cut(s) 133, 614
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 2 cut(s) 145, 766
AfaI GTAC 2 cut(s) 209, 854
AfiI CCNNNNNNNGG 2 cut(s) 99, 522
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 2 cut(s) 773, 878
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 8 cut(s) 82, 114, 288, 449, 489, 615, 781, 868
AluI AGCT 8 cut(s) 82, 114, 288, 449, 489, 615, 781, 868
AlwI GGATC 2 cut(s) 133, 614
AoxI GGCC 3 cut(s) 101, 217, 312
ApeKI GCWGC 4 cut(s) 161, 260, 481, 635
ApoI RAATTY 2 cut(s) 145, 766
AspS9I GGNCC 2 cut(s) 102, 312
AsuHPI GGTGA 5 cut(s) 76, 558, 725, 780, 853
BaeGI GKGCMC 1 cut(s) 552
BaeI ACNNNNGTAYC 2 cut(s) 844, 877
BbsI GAAGAC 1 cut(s) 657
BbvI GCAGC 4 cut(s) 148, 247, 468, 622
BccI CCATC 4 cut(s) 82, 412, 577, 838
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 100
BclI TGATCA 2 cut(s) 397, 471
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 731
BfuAI ACCTGC 1 cut(s) 765
BisI GCNGC 5 cut(s) 162, 261, 482, 561, 636
BlsI GCNGC 5 cut(s) 163, 262, 483, 562, 637
Bme1390I CCNGG 1 cut(s) 100
BmgT120I GGNCC 2 cut(s) 102, 312
BmiI GGNNCC 3 cut(s) 313, 606, 752
BmrFI CCNGG 1 cut(s) 100
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 2 cut(s) 447, 695
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 1 cut(s) 797
Bsa29I ATCGAT 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 709, 816
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 522
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 4 cut(s) 250, 313, 414, 780
Bse3DI GCAATG 2 cut(s) 194, 255
BseBI CCWGG 1 cut(s) 100
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 709, 816
BseGI GGATG 3 cut(s) 74, 427, 710
BseLI CCNNNNNNNGG 2 cut(s) 99, 522
BseMI GCAATG 2 cut(s) 194, 255
BseMII CTCAG 4 cut(s) 162, 228, 466, 752
BseNI ACTGG 4 cut(s) 250, 313, 414, 780
BseRI GAGGAG 2 cut(s) 311, 371
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 4 cut(s) 148, 247, 468, 622
BseYI CCCAGC 3 cut(s) 78, 272, 638
BsgI GTGCAG 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 563
BshFI GGCC 3 cut(s) 103, 219, 314
BshVI ATCGAT 1 cut(s) 137
BsiSI CCGG 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 99, 522
BsmI GAATGC 2 cut(s) 668, 817
BsnI GGCC 3 cut(s) 103, 219, 314
Bsp1286I GDGCHC 1 cut(s) 552
Bsp143I GATC 5 cut(s) 134, 138, 397, 471, 619
Bsp19I CCATGG 1 cut(s) 816
BspACI CCGC 3 cut(s) 11, 561, 850
BspANI GGCC 3 cut(s) 103, 219, 314
BspCNI CTCAG 4 cut(s) 163, 229, 465, 753
BspDI ATCGAT 1 cut(s) 137
BspFNI CGCG 1 cut(s) 563
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 313, 606, 752
BspMI ACCTGC 1 cut(s) 765
BspPI GGATC 2 cut(s) 133, 614
BsrDI GCAATG 2 cut(s) 194, 255
BsrFI RCCGGY 1 cut(s) 582
BsrI ACTGG 4 cut(s) 250, 313, 414, 780
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 2 cut(s) 709, 816
BssMI GATC 5 cut(s) 134, 138, 397, 471, 619
BssT1I CCWWGG 2 cut(s) 709, 816
Bst2UI CCWGG 1 cut(s) 100
Bst4CI ACNGT 2 cut(s) 88, 168
BstC8I GCNNGC 5 cut(s) 40, 159, 251, 491, 698
BstDEI CTNAG 4 cut(s) 171, 237, 452, 761
BstDSI CCRYGG 1 cut(s) 816
BstF5I GGATG 3 cut(s) 74, 427, 710
BstFNI CGCG 1 cut(s) 563
BstKTI GATC 5 cut(s) 137, 141, 400, 474, 622
BstMBI GATC 5 cut(s) 134, 138, 397, 471, 619
BstMWI GCNNNNNNNGC 4 cut(s) 269, 490, 856, 865
BstNI CCWGG 1 cut(s) 100
BstNSI RCATGY 2 cut(s) 42, 184
BstSCI CCNGG 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 731
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 1 cut(s) 563
BstV1I GCAGC 4 cut(s) 148, 247, 468, 622
BstV2I GAAGAC 1 cut(s) 657
BstXI CCANNNNNNTGG 1 cut(s) 823
Bsu15I ATCGAT 1 cut(s) 137
BsuRI GGCC 3 cut(s) 103, 219, 314
BsuTUI ATCGAT 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 816
BtsCI GGATG 3 cut(s) 74, 427, 710
BtsIMutI CAGTG 3 cut(s) 57, 243, 467
BveI ACCTGC 1 cut(s) 765
Cac8I GCNNGC 5 cut(s) 40, 159, 251, 491, 698
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 2 cut(s) 102, 312
ClaI ATCGAT 1 cut(s) 137
CseI GACGC 2 cut(s) 552, 848
Csp6I GTAC 2 cut(s) 208, 853
CviAII CATG 7 cut(s) 22, 39, 181, 383, 401, 701, 817
CviQI GTAC 2 cut(s) 208, 853
DdeI CTNAG 4 cut(s) 171, 237, 452, 761
DpnI GATC 5 cut(s) 136, 140, 399, 473, 621
DpnII GATC 5 cut(s) 134, 138, 397, 471, 619
EaeI YGGCCR 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 709, 816
Eco32I GATATC 2 cut(s) 123, 538
EcoO109I RGGNCCY 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 98
EcoRV GATATC 2 cut(s) 123, 538
EcoT14I CCWWGG 2 cut(s) 709, 816
ErhI CCWWGG 2 cut(s) 709, 816
FaeI CATG 7 cut(s) 25, 42, 184, 386, 404, 704, 820
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 7 cut(s) 21, 38, 180, 382, 400, 700, 816
FbaI TGATCA 2 cut(s) 397, 471
Fnu4HI GCNGC 5 cut(s) 162, 261, 482, 561, 636
FokI GGATG 3 cut(s) 61, 434, 717
Fsp4HI GCNGC 5 cut(s) 162, 261, 482, 561, 636
FspBI CTAG 1 cut(s) 285
GluI GCNGC 5 cut(s) 162, 261, 482, 561, 636
GsaI CCCAGC 3 cut(s) 82, 276, 642
GsuI CTGGAG 1 cut(s) 797
HaeIII GGCC 3 cut(s) 103, 219, 314
HapII CCGG 1 cut(s) 583
HgaI GACGC 2 cut(s) 552, 848
Hin1II CATG 7 cut(s) 25, 42, 184, 386, 404, 704, 820
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 2 cut(s) 200, 233
HpaII CCGG 1 cut(s) 583
HphI GGTGA 5 cut(s) 76, 558, 725, 780, 853
Hpy188I TCNGA 6 cut(s) 120, 143, 172, 238, 355, 455
Hpy188III TCNNGA 4 cut(s) 401, 541, 623, 863
Hpy99I CGWCG 2 cut(s) 92, 864
HpyAV CCTTC 4 cut(s) 79, 169, 520, 538
HpyCH4III ACNGT 2 cut(s) 88, 168
HpyCH4V TGCA 6 cut(s) 130, 164, 187, 438, 460, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 490, 856, 865
HpyF3I CTNAG 4 cut(s) 171, 237, 452, 761
Hsp92II CATG 7 cut(s) 25, 42, 184, 386, 404, 704, 820
Ksp22I TGATCA 2 cut(s) 397, 471
Kzo9I GATC 5 cut(s) 134, 138, 397, 471, 619
LmnI GCTCC 5 cut(s) 358, 604, 750, 778, 865
Lsp1109I GCAGC 4 cut(s) 148, 247, 468, 622
LweI GCATC 2 cut(s) 447, 695
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 4 cut(s) 94, 241, 326, 564
MalI GATC 5 cut(s) 136, 140, 399, 473, 621
MboI GATC 5 cut(s) 134, 138, 397, 471, 619
MboII GAAGA 4 cut(s) 51, 403, 659, 662
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 2 cut(s) 145, 766
MlyI GAGTC 1 cut(s) 194
MnlI CCTC 7 cut(s) 232, 332, 349, 474, 514, 600, 683
MslI CAYNNNNRTG 1 cut(s) 821
MspI CCGG 1 cut(s) 583
MspR9I CCNGG 1 cut(s) 100
Mva1269I GAATGC 2 cut(s) 668, 817
MvaI CCWGG 1 cut(s) 100
MvnI CGCG 1 cut(s) 563
MwoI GCNNNNNNNGC 4 cut(s) 269, 490, 856, 865
NcoI CCATGG 1 cut(s) 816
NdeII GATC 5 cut(s) 134, 138, 397, 471, 619
NlaIII CATG 7 cut(s) 25, 42, 184, 386, 404, 704, 820
NlaIV GGNNCC 3 cut(s) 313, 606, 752
NmuCI GTSAC 4 cut(s) 94, 241, 326, 564
NspI RCATGY 2 cut(s) 42, 184
PaeI GCATGC 1 cut(s) 42
PagI TCATGA 1 cut(s) 400
PaqCI CACCTGC 1 cut(s) 765
PciI ACATGT 1 cut(s) 180
PctI GAATGC 2 cut(s) 668, 817
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 5 cut(s) 163, 262, 483, 562, 637
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PscI ACATGT 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 98
PspFI CCCAGC 3 cut(s) 78, 272, 638
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 313, 606, 752
PspPI GGNCC 2 cut(s) 102, 312
RsaI GTAC 2 cut(s) 209, 854
RsaNI GTAC 2 cut(s) 208, 853
RseI CAYNNNNRTG 1 cut(s) 821
SatI GCNGC 5 cut(s) 162, 261, 482, 561, 636
Sau3AI GATC 5 cut(s) 134, 138, 397, 471, 619
Sau96I GGNCC 2 cut(s) 102, 312
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 2 cut(s) 447, 695
SfcI CTRYAG 1 cut(s) 731
SmiMI CAYNNNNRTG 1 cut(s) 821
SphI GCATGC 1 cut(s) 42
Sse9I AATT 2 cut(s) 145, 766
SsiI CCGC 3 cut(s) 11, 561, 850
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 98
StyI CCWWGG 2 cut(s) 709, 816
TaaI ACNGT 2 cut(s) 88, 168
TaqI TCGA 1 cut(s) 137
TasI AATT 2 cut(s) 145, 766
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 563
TfiI GAWTC 1 cut(s) 233
TscAI CASTG 3 cut(s) 57, 250, 474
TseFI GTSAC 4 cut(s) 94, 241, 326, 564
TseI GCWGC 4 cut(s) 161, 260, 481, 635
Tsp45I GTSAC 4 cut(s) 94, 241, 326, 564
TspDTI ATGAA 2 cut(s) 184, 389
TspGWI ACGGA 2 cut(s) 314, 818
TspRI CASTG 3 cut(s) 57, 250, 474
XapI RAATTY 2 cut(s) 145, 766
XceI RCATGY 2 cut(s) 42, 184
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.