pycom16g12040

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004891
Physical Location & Seq
Forward (+)
8396190 .. 8397826
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1200 bp
ATGAAGAGTGATTTTACACAGGGGAAGGAACTTTTGAGACCATCTATTACCCGGTTTGCTTCTAGCTTTGCTACCTTACAAAGCTTGTTGGACCACAAGACTGGTCTTAAAAGAATGTTTCAATCAAACAAATGGATTTCATCACAGTGCTCCAAATCATTTGAGGGAAAACAGGTGGAAAATATCGTCTTAAATGCTGCATTTTGGAAGAAGTTACAGTTTGTTAGGAATTCAGTGAACCCGATAATGCAAATTCTTCAAAAGGTTGAGAGTGGTGACTGCTTGCCAATGTCATCTATATACAATGACATGTGCAGGGCAAAGATTGCAATAAAAGCCATTCATGGTAATAAAGTCCGTAAGTATGAACCATTTTGGAGCGTTATAGAAAGTCATTGGAACTCGCTGTTCTACCACCCCGTATATGTAGCTGCTTACTACTTAAATCCATCATACAGATATCGACCTGATTTTATGGCGCATACTGAGGTGATGCGTGGACTTAACGAATGCATTGTTCGGCTGGAGCCAGACCATTCAAATAGGATTTCTGCATCTATGCAGATTTCTGATTACAATTCTGCTAAAGCTGATTTTGGAACCGAATTGGCAATCAGTACAAGAACAGAGCTTGATCCAGCTGCATGGTGGCAGCAACATGGGATAAGTTGCTTAGAGCTGCAGCGTATAGCTGTGCGTATATTGAGTCAGACATGCTCTTCTTTTGGGTGTGAGCATAACTGGAGTATATACGATCAATTGTACAGTCTAAGAAGTAATCGTTTAGCTCAAAAAAGATTAAACGAGCTCATCTACGTTCATTACAATTTGCGTCTTAGAGAACAGCAATTACGAAGAAGGCCGGAAAATTCAATCTCCCTCGACAATGTGCTGATAGAACGGTTGCTAGATGACTGGATTGTAGATGCTGCGGAAAATGACTTGCTAGAAAATGAGGAGGTCCTTTGCAACGAAATTGAACAGGTTGATGAATACGAGAATGATCTTGTTGATTACAAATGTGGAATGGCAAATGGAGAGACGAGGAATGGATCCATTGAGCTGGTAACCTTGGCTGATGGAGATGTCAATCCAGCCAATGCTGGTGTTGCCACCGATGATGATGATGATGAGGAGGAGGAGGAGGAGGAGGAGGCGGATGGCGATATGAGCTTTTTCCATATTGACATGAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

400

Amino Acids

46.03

Weight (kDa)

4.87

Isoelectric Point (pI)

53.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 207 - 277 4.2e-12 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 932, 1157
AclWI GGATC 3 cut(s) 629, 1047, 1060
AcsI RAATTY 3 cut(s) 229, 252, 868
AfaI GTAC 2 cut(s) 619, 764
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 5 cut(s) 122, 260, 540, 873, 980
Alw21I GWGCWC 2 cut(s) 152, 810
Alw26I GTCTC 2 cut(s) 31, 1034
AlwI GGATC 3 cut(s) 629, 1047, 1060
AoxI GGCC 1 cut(s) 860
ApeKI GCWGC 7 cut(s) 197, 431, 641, 652, 679, 682, 929
ApoI RAATTY 3 cut(s) 229, 252, 868
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 2 cut(s) 91, 961
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 287, 502
AvaII GGWCC 2 cut(s) 91, 961
BamHI GGATCC 1 cut(s) 1052
BanII GRGCYC 1 cut(s) 810
Bbv12I GWGCWC 2 cut(s) 152, 810
BbvI GCAGC 7 cut(s) 184, 418, 628, 664, 666, 694, 916
BccI CCATC 4 cut(s) 49, 457, 1073, 1154
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 31, 1034
BfaI CTAG 3 cut(s) 63, 908, 947
BfmI CTRYAG 1 cut(s) 680
BisI GCNGC 7 cut(s) 198, 432, 642, 653, 680, 683, 930
BlsI GCNGC 7 cut(s) 199, 433, 643, 654, 681, 684, 931
Bme1390I CCNGG 1 cut(s) 52
Bme18I GGWCC 2 cut(s) 91, 961
BmgT120I GGNCC 2 cut(s) 91, 961
BmiI GGNNCC 3 cut(s) 528, 601, 1054
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 3 cut(s) 483, 563, 916
BpmI CTGGAG 2 cut(s) 545, 763
BpuMI CCSGG 1 cut(s) 52
BsaBI GATNNNNATC 1 cut(s) 1089
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 1071
Bse1I ACTGG 3 cut(s) 106, 746, 920
Bse8I GATNNNNATC 1 cut(s) 1089
BseDI CCNNGG 1 cut(s) 1071
BseGI GGATG 1 cut(s) 1165
BseJI GATNNNNATC 1 cut(s) 1089
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 3 cut(s) 106, 746, 920
BseRI GAGGAG 8 cut(s) 971, 1148, 1151, 1154, 1157, 1160, 1163, 1166
BseXI GCAGC 7 cut(s) 184, 418, 628, 664, 666, 694, 916
BsgI GTGCAG 1 cut(s) 334
BshFI GGCC 1 cut(s) 862
BsiHKAI GWGCWC 2 cut(s) 152, 810
BsiSI CCGG 2 cut(s) 52, 863
BsmAI GTCTC 2 cut(s) 31, 1034
BsmBI CGTCTC 1 cut(s) 1034
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 1 cut(s) 862
Bso31I GGTCTC 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 152, 810
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 4 cut(s) 634, 754, 1003, 1052
BspACI CCGC 2 cut(s) 932, 1157
BspANI GGCC 1 cut(s) 862
BspCNI CTCAG 1 cut(s) 478
BspLI GGNNCC 3 cut(s) 528, 601, 1054
BspMAI CTGCAG 1 cut(s) 684
BspPI GGATC 3 cut(s) 629, 1047, 1060
BspQI GCTCTTC 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 3 cut(s) 106, 746, 920
BssECI CCNNGG 1 cut(s) 1071
BssMI GATC 4 cut(s) 634, 754, 1003, 1052
BssT1I CCWWGG 1 cut(s) 1071
Bst4CI ACNGT 4 cut(s) 147, 219, 767, 903
Bst6I CTCTTC 1 cut(s) 724
BstAPI GCANNNNNTGC 1 cut(s) 326
BstAUI TGTACA 1 cut(s) 762
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 4 cut(s) 486, 673, 770, 836
BstEII GGTNACC 1 cut(s) 1066
BstF5I GGATG 1 cut(s) 1165
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 4 cut(s) 637, 757, 1006, 1055
BstMAI GTCTC 2 cut(s) 31, 1034
BstMBI GATC 4 cut(s) 634, 754, 1003, 1052
BstMWI GCNNNNNNNGC 4 cut(s) 326, 335, 1109, 1170
BstNSI RCATGY 2 cut(s) 313, 717
BstPI GGTNACC 1 cut(s) 1066
BstSCI CCNGG 1 cut(s) 50
BstSFI CTRYAG 1 cut(s) 680
BstV1I GCAGC 7 cut(s) 184, 418, 628, 664, 666, 694, 916
BstX2I RGATCY 1 cut(s) 1052
BstXI CCANNNNNNTGG 3 cut(s) 101, 645, 1063
BstYI RGATCY 1 cut(s) 1052
BsuRI GGCC 1 cut(s) 862
BtsCI GGATG 1 cut(s) 1165
BtsIMutI CAGTG 2 cut(s) 152, 240
Cac8I GCNNGC 1 cut(s) 284
CfoI GCGC 1 cut(s) 481
Cfr13I GGNCC 2 cut(s) 91, 961
CseI GACGC 1 cut(s) 821
Csp6I GTAC 2 cut(s) 618, 763
CviAII CATG 6 cut(s) 310, 344, 645, 659, 714, 1189
CviQI GTAC 2 cut(s) 618, 763
DdeI CTNAG 4 cut(s) 486, 673, 770, 836
DpnI GATC 4 cut(s) 636, 756, 1005, 1054
DpnII GATC 4 cut(s) 634, 754, 1003, 1052
Eam1104I CTCTTC 1 cut(s) 724
EarI CTCTTC 1 cut(s) 724
EciI GGCGGA 1 cut(s) 1172
Ecl136II GAGCTC 1 cut(s) 808
Eco130I CCWWGG 1 cut(s) 1071
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 31
Eco32I GATATC 1 cut(s) 461
Eco47I GGWCC 2 cut(s) 91, 961
Eco53kI GAGCTC 1 cut(s) 808
Eco91I GGTNACC 1 cut(s) 1066
EcoICRI GAGCTC 1 cut(s) 808
EcoO109I RGGNCCY 1 cut(s) 961
EcoO65I GGTNACC 1 cut(s) 1066
EcoRI GAATTC 1 cut(s) 229
EcoRV GATATC 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 1071
EcoT22I ATGCAT 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 1 cut(s) 1071
Esp3I CGTCTC 1 cut(s) 1034
FaeI CATG 6 cut(s) 313, 347, 648, 662, 717, 1192
FatI CATG 6 cut(s) 309, 343, 644, 658, 713, 1188
Fnu4HI GCNGC 7 cut(s) 198, 432, 642, 653, 680, 683, 930
FokI GGATG 1 cut(s) 1172
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 7 cut(s) 198, 432, 642, 653, 680, 683, 930
FspBI CTAG 3 cut(s) 63, 908, 947
GlaI GCGC 1 cut(s) 480
GluI GCNGC 7 cut(s) 198, 432, 642, 653, 680, 683, 930
GsuI CTGGAG 2 cut(s) 545, 763
HaeIII GGCC 1 cut(s) 862
HapII CCGG 2 cut(s) 52, 863
HgaI GACGC 1 cut(s) 821
HhaI GCGC 1 cut(s) 481
Hin1II CATG 6 cut(s) 313, 347, 648, 662, 717, 1192
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 1 cut(s) 706
HpaII CCGG 2 cut(s) 52, 863
HphI GGTGA 2 cut(s) 287, 502
Hpy166II GTNNAC 2 cut(s) 238, 500
Hpy188I TCNGA 2 cut(s) 571, 711
Hpy8I GTNNAC 2 cut(s) 238, 500
HpyAV CCTTC 2 cut(s) 19, 852
HpyCH4III ACNGT 4 cut(s) 147, 219, 767, 903
HpyCH4IV ACGT 1 cut(s) 816
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 335, 1109, 1170
HpyF3I CTNAG 4 cut(s) 486, 673, 770, 836
HpySE526I ACGT 1 cut(s) 816
Hsp92II CATG 6 cut(s) 313, 347, 648, 662, 717, 1192
HspAI GCGC 1 cut(s) 479
Kzo9I GATC 4 cut(s) 634, 754, 1003, 1052
LguI GCTCTTC 1 cut(s) 724
LmnI GCTCC 3 cut(s) 155, 378, 526
Lsp1109I GCAGC 7 cut(s) 184, 418, 628, 664, 666, 694, 916
LweI GCATC 3 cut(s) 483, 563, 916
MaeI CTAG 3 cut(s) 63, 908, 947
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 3 cut(s) 213, 275, 1066
MalI GATC 4 cut(s) 636, 756, 1005, 1054
MboI GATC 4 cut(s) 634, 754, 1003, 1052
MboII GAAGA 5 cut(s) 16, 220, 248, 711, 867
MfeI CAATTG 1 cut(s) 758
MflI RGATCY 1 cut(s) 1052
MhlI GDGCHC 2 cut(s) 152, 810
MluCI AATT 9 cut(s) 229, 252, 577, 605, 758, 826, 848, 868, 975
MlyI GAGTC 1 cut(s) 715
MmeI TCCRAC 1 cut(s) 69
Mph1103I ATGCAT 1 cut(s) 515
MseI TTAA 5 cut(s) 108, 191, 443, 504, 800
MslI CAYNNNNRTG 1 cut(s) 145
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 2 cut(s) 52, 863
MspR9I CCNGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 758
Mva1269I GAATGC 1 cut(s) 515
MwoI GCNNNNNNNGC 4 cut(s) 326, 335, 1109, 1170
NciI CCSGG 1 cut(s) 52
NdeII GATC 4 cut(s) 634, 754, 1003, 1052
NlaIII CATG 6 cut(s) 313, 347, 648, 662, 717, 1192
NlaIV GGNNCC 3 cut(s) 528, 601, 1054
NmuCI GTSAC 1 cut(s) 275
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 2 cut(s) 313, 717
PciI ACATGT 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 724
PctI GAATGC 1 cut(s) 515
PkrI GCNGC 7 cut(s) 199, 433, 643, 654, 681, 684, 931
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
PpuMI RGGWCCY 1 cut(s) 961
PscI ACATGT 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 810
Psp5II RGGWCCY 1 cut(s) 961
PspEI GGTNACC 1 cut(s) 1066
PspN4I GGNNCC 3 cut(s) 528, 601, 1054
PspPI GGNCC 2 cut(s) 91, 961
PspPPI RGGWCCY 1 cut(s) 961
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstI CTGCAG 1 cut(s) 684
PsuI RGATCY 1 cut(s) 1052
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 2 cut(s) 619, 764
RsaNI GTAC 2 cut(s) 618, 763
RseI CAYNNNNRTG 1 cut(s) 145
SacI GAGCTC 1 cut(s) 810
SapI GCTCTTC 1 cut(s) 724
SaqAI TTAA 5 cut(s) 108, 191, 443, 504, 800
SatI GCNGC 7 cut(s) 198, 432, 642, 653, 680, 683, 930
Sau3AI GATC 4 cut(s) 634, 754, 1003, 1052
Sau96I GGNCC 2 cut(s) 91, 961
SchI GAGTC 1 cut(s) 715
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 2 cut(s) 152, 810
SfaNI GCATC 3 cut(s) 483, 563, 916
SfcI CTRYAG 1 cut(s) 680
SinI GGWCC 2 cut(s) 91, 961
SmiMI CAYNNNNRTG 1 cut(s) 145
Sse9I AATT 9 cut(s) 229, 252, 577, 605, 758, 826, 848, 868, 975
SsiI CCGC 2 cut(s) 932, 1157
SspMI CTAG 3 cut(s) 63, 908, 947
SstI GAGCTC 1 cut(s) 810
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 4 cut(s) 147, 219, 767, 903
TaiI ACGT 1 cut(s) 819
TaqI TCGA 2 cut(s) 463, 882
TasI AATT 9 cut(s) 229, 252, 577, 605, 758, 826, 848, 868, 975
TatI WGTACW 2 cut(s) 617, 762
Tru1I TTAA 5 cut(s) 108, 191, 443, 504, 800
Tru9I TTAA 5 cut(s) 108, 191, 443, 504, 800
TscAI CASTG 2 cut(s) 152, 240
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 7 cut(s) 197, 431, 641, 652, 679, 682, 929
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 6 cut(s) 17, 129, 332, 381, 809, 1005
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 2 cut(s) 152, 240
VpaK11BI GGWCC 2 cut(s) 91, 961
XapI RAATTY 3 cut(s) 229, 252, 868
XceI RCATGY 2 cut(s) 313, 717
XcmI CCANNNNNNNNNTGG 1 cut(s) 645
XspI CTAG 3 cut(s) 63, 908, 947
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.