pycom16g12880

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014620
Physical Location & Seq
Reverse (-)
9097628 .. 9100843
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1347 bp
ATGGCACAAATGGCAAAACACGAAGAACTCCCCATTCCAATTCACGACACCCTCAACGCCGTCTACGGCGACTCCTCCCAACTCGACGAGACCCAGCTACGATTCAACCGGCTGAAGTCCAAGTTCCAGCAGGTTTTCGGTCACCCTCCCCAACTCTTCGCTCGCTCTCCAGGGAGGGTGAACTTGATCGGAGAGCACATAGACTACGAGGGCTACTCGGTGCTGCCGATGGCCATACGACAGGACACCATTGTCGCAATTCGGAAGCGCGACGACGGAGACGCCGAGAAAGTTCTCAGAATCGCCAATATCAACGACAAGTACCAAATTTGCACTTATCCCGCCGACCCGACCCAGGAAATTGACTTGAAGAATCACAAATGGGGGCACTATTTCATTTGTGGGTACAAAGGTTTTTACGAATTTGCCAAATCGAAGGGAGAAAATGTTAGAGCAGTTGGGCTTGATGTTCTTATTGATGGAACAGTTCCTACAGGCTCCGGACTATCGAGCTCTGCTGCATTAGTTTGTGCTTCTACGATTGCGATAATGGCTGCTTTTGATGTGAATTTCCCCAAGAAAGTGGTTGCCCAACTTACGTGCGAGTGTGAGCGTCACATTGGCACACAATCTGGTGGAATGGATCAGGCAATCTCTGTGATGGCCAAGACTGGGTTTGCAGAACTAATTGATTTTAACCCTGTTCGTGCTACTGATGTGCAACTTCCGGCTGGTGGGACTTTTGTTGTGGCCAATTCTTTGGCAGATTCTCAGAAAGCAGTAACTGCTGCCACAAATTACAATAACCGAGTTGTTGAATGTCGTTTGGCTGCTATTGTGCTTGGGATAAAGTTGGGGATGAAACCGCAAGAGGCAATATCAAGTGTCAAAACTCTTTCCGACGTTGAAGGGTTGTGCATATCATTTGCTAGTACTCGTGGGTCAACAGATCCTGTCGTGGCTGTAAAGGAGAATTTAAAAGAAGAACCTTATACAGCTGGGGAAATAGAGGAAATTCTTGGGGAGAGTCTGCCCTCTATCTTGAAAGATTCCTCGTCTTCTTTGGATGTTTTGAACGTTGCAAAGAACTTCAAGTTATCTCAGAGAGCCTCCCATGTGTACTCTGAAGCCAAGCGGGTACATGCTTTCAAGGACGCCGTCTCATCAACAGTAAGTGATGAGGATAAGCTAAAGAAGCTTGGGGATCTTATGAATGATAGCCACTACAGCTGCAGTGAACGCTTCTACCAGTCGCGAATTGACAAAGGCGTCATAAACAATAACGATCTCGGCCTATATGTTTTTGCTTCCAAGCCGTCGAGCGGTGCTGCTATCTTCAAGTTTTAG

Protein Analysis

449

Amino Acids

48.89

Weight (kDa)

6.1

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 41 - 89 1e-23 Galactokinase galactose-binding signature
GHMP_kinases_N PF00288 147 - 222 5.6e-16 GHMP kinases N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014620)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06580
fragaria_vesca FvH4_4g20200 FvH4_4g20200
malus_domestica MD13G1150000.v1.1 MD16G1150400.v1.1
prunus_persica Prupe.1G114000_v2.0.a1
pyrus_communis pycom13g12920 pycom16g12880
rosa_chinensis RchiOBHm_Chr4g0425841
rosa_laevigata RLG00000007336
rosa_multiflora Rmu_sc0007063.1_g000006 Rmu_sc0009636.1_g000002
rosa_roxburghii Rroxscaffold_5G00367820
rosa_rugosa Rorug04G0206700
rosa_samantha Rh4CG282200 Rh4DG266100
rosa_wichuraiana Rw4G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 251, 1268
Acc36I ACCTGC 1 cut(s) 121
AccBSI CCGCTC 1 cut(s) 1323
AccI GTMKAC 1 cut(s) 63
AccII CGCG 2 cut(s) 270, 1255
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 4 cut(s) 342, 866, 1135, 1323
AclI AACGTT 1 cut(s) 1077
AclWI GGATC 3 cut(s) 651, 944, 1212
AcoI YGGCCR 3 cut(s) 231, 663, 750
AcsI RAATTY 5 cut(s) 327, 422, 568, 973, 1014
AcuI CTGAAG 2 cut(s) 134, 1146
AcyI GRCGYC 3 cut(s) 282, 1155, 1269
AfaI GTAC 5 cut(s) 323, 407, 934, 1121, 1140
AfiI CCNNNNNNNGG 4 cut(s) 355, 672, 734, 1322
AgsI TTSAA 9 cut(s) 106, 370, 818, 908, 1045, 1075, 1093, 1150, 1339
AjnI CCWGG 2 cut(s) 169, 354
AluBI AGCT 6 cut(s) 97, 513, 998, 1189, 1198, 1230
AluI AGCT 6 cut(s) 97, 513, 998, 1189, 1198, 1230
Alw21I GWGCWC 2 cut(s) 198, 515
Alw26I GTCTC 3 cut(s) 83, 273, 1165
AlwI GGATC 3 cut(s) 651, 944, 1212
AlwNI CAGNNNCTG 2 cut(s) 785, 953
Aor13HI TCCGGA 1 cut(s) 500
AoxI GGCC 4 cut(s) 231, 663, 750, 1291
ApeKI GCWGC 7 cut(s) 223, 518, 554, 788, 830, 1230, 1328
ApoI RAATTY 5 cut(s) 327, 422, 568, 973, 1014
AspLEI GCGC 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 134, 190
BaeGI GKGCMC 1 cut(s) 390
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BalI TGGCCA 3 cut(s) 233, 665, 752
BanII GRGCYC 1 cut(s) 515
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 1 cut(s) 1050
Bbv12I GWGCWC 2 cut(s) 198, 515
BbvI GCAGC 7 cut(s) 210, 505, 541, 775, 817, 1217, 1315
BccI CCATC 3 cut(s) 223, 473, 655
BceAI ACGGC 4 cut(s) 44, 82, 1142, 1300
BciT130I CCWGG 2 cut(s) 171, 356
BcoDI GTCTC 3 cut(s) 83, 273, 1165
BfaI CTAG 1 cut(s) 930
BfmI CTRYAG 3 cut(s) 492, 1225, 1231
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 7 cut(s) 224, 519, 555, 789, 831, 1231, 1329
BlsI GCNGC 7 cut(s) 225, 520, 556, 790, 832, 1232, 1330
BmcAI AGTACT 1 cut(s) 934
Bme1390I CCNGG 2 cut(s) 171, 356
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 2 cut(s) 171, 356
BmrI ACTGGG 1 cut(s) 681
BmuI ACTGGG 1 cut(s) 681
BpiI GAAGAC 1 cut(s) 1050
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 600
BsaHI GRCGYC 3 cut(s) 282, 1155, 1269
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 170, 354
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 432, 462
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 672, 734, 1322
Bse118I RCCGGY 1 cut(s) 108
Bse1I ACTGG 2 cut(s) 676, 1249
BseAI TCCGGA 1 cut(s) 500
BseBI CCWGG 2 cut(s) 171, 356
BseDI CCNNGG 2 cut(s) 170, 354
BseGI GGATG 2 cut(s) 864, 1072
BseLI CCNNNNNNNGG 4 cut(s) 355, 672, 734, 1322
BseMII CTCAG 3 cut(s) 310, 785, 1115
BseNI ACTGG 2 cut(s) 676, 1249
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 7 cut(s) 210, 505, 541, 775, 817, 1217, 1315
BseYI CCCAGC 2 cut(s) 93, 998
Bsh1236I CGCG 2 cut(s) 270, 1255
BshFI GGCC 4 cut(s) 233, 665, 752, 1293
BsiHKAI GWGCWC 2 cut(s) 198, 515
BsiSI CCGG 3 cut(s) 109, 501, 728
BslFI GGGAC 1 cut(s) 751
BslI CCNNNNNNNGG 4 cut(s) 355, 672, 734, 1322
BsmAI GTCTC 3 cut(s) 83, 273, 1165
BsmBI CGTCTC 2 cut(s) 273, 1165
BsmFI GGGAC 1 cut(s) 751
BsnI GGCC 4 cut(s) 233, 665, 752, 1293
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 3 cut(s) 198, 390, 515
Bsp13I TCCGGA 1 cut(s) 500
Bsp143I GATC 5 cut(s) 186, 643, 949, 1204, 1285
Bsp68I TCGCGA 1 cut(s) 1255
BspACI CCGC 4 cut(s) 342, 866, 1135, 1323
BspANI GGCC 4 cut(s) 233, 665, 752, 1293
BspCNI CTCAG 3 cut(s) 309, 784, 1114
BspEI TCCGGA 1 cut(s) 500
BspFNI CGCG 2 cut(s) 270, 1255
BspLI GGNNCC 1 cut(s) 499
BspMAI CTGCAG 1 cut(s) 1235
BspMI ACCTGC 1 cut(s) 121
BspPI GGATC 3 cut(s) 651, 944, 1212
BspTNI GGTCTC 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 1323
BsrFI RCCGGY 1 cut(s) 108
BsrI ACTGG 2 cut(s) 676, 1249
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 2 cut(s) 170, 354
BssMI GATC 5 cut(s) 186, 643, 949, 1204, 1285
BssNI GRCGYC 3 cut(s) 282, 1155, 1269
BssSI CACGAG 1 cut(s) 936
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 2 cut(s) 171, 356
Bst4CI ACNGT 2 cut(s) 487, 1171
Bst6I CTCTTC 1 cut(s) 161
BstACI GRCGYC 3 cut(s) 282, 1155, 1269
BstAPI GCANNNNNTGC 1 cut(s) 785
BstBAI YACGTR 1 cut(s) 600
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 3 cut(s) 296, 771, 1101
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 2 cut(s) 864, 1072
BstFNI CGCG 2 cut(s) 270, 1255
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 5 cut(s) 189, 646, 952, 1207, 1288
BstMAI GTCTC 3 cut(s) 83, 273, 1165
BstMBI GATC 5 cut(s) 186, 643, 949, 1204, 1285
BstMWI GCNNNNNNNGC 6 cut(s) 11, 551, 785, 1195, 1227, 1239
BstNI CCWGG 2 cut(s) 171, 356
BstNSI RCATGY 1 cut(s) 1145
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 169, 354
BstSFI CTRYAG 3 cut(s) 492, 1225, 1231
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 2 cut(s) 270, 1255
BstV1I GCAGC 7 cut(s) 210, 505, 541, 775, 817, 1217, 1315
BstV2I GAAGAC 1 cut(s) 1050
BstX2I RGATCY 2 cut(s) 949, 1204
BstXI CCANNNNNNTGG 2 cut(s) 583, 760
BstYI RGATCY 2 cut(s) 949, 1204
BsuRI GGCC 4 cut(s) 233, 665, 752, 1293
BtsCI GGATG 2 cut(s) 864, 1072
BtsI GCAGTG 1 cut(s) 1240
BtsIMutI CAGTG 1 cut(s) 1240
BtuMI TCGCGA 1 cut(s) 1255
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 163
CaiI CAGNNNCTG 2 cut(s) 785, 953
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 108
CseI GACGC 4 cut(s) 290, 602, 1163, 1258
Csp6I GTAC 5 cut(s) 322, 406, 933, 1120, 1139
CviAII CATG 2 cut(s) 1115, 1142
CviQI GTAC 5 cut(s) 322, 406, 933, 1120, 1139
DdeI CTNAG 3 cut(s) 296, 771, 1101
DpnI GATC 5 cut(s) 188, 645, 951, 1206, 1287
DpnII GATC 5 cut(s) 186, 643, 949, 1204, 1285
DraI TTTAAA 1 cut(s) 978
DrdI GACNNNNNNGTC 2 cut(s) 251, 1268
DseDI GACNNNNNNGTC 2 cut(s) 251, 1268
EaeI YGGCCR 3 cut(s) 231, 663, 750
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 515
Eco31I GGTCTC 1 cut(s) 83
Eco53kI GAGCTC 1 cut(s) 513
Eco57I CTGAAG 2 cut(s) 134, 1146
Eco91I GGTNACC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 513
EcoO65I GGTNACC 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 169, 354
EcoT38I GRGCYC 1 cut(s) 515
Esp3I CGTCTC 2 cut(s) 273, 1165
FaeI CATG 2 cut(s) 1118, 1145
FaqI GGGAC 1 cut(s) 751
FatI CATG 2 cut(s) 1114, 1141
FauI CCCGC 2 cut(s) 349, 1128
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 7 cut(s) 224, 519, 555, 789, 831, 1231, 1329
FokI GGATG 2 cut(s) 871, 1079
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 7 cut(s) 224, 519, 555, 789, 831, 1231, 1329
FspBI CTAG 1 cut(s) 930
GlaI GCGC 1 cut(s) 269
GluI GCNGC 7 cut(s) 224, 519, 555, 789, 831, 1231, 1329
GsaI CCCAGC 2 cut(s) 97, 1002
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 4 cut(s) 233, 665, 752, 1293
HapII CCGG 3 cut(s) 109, 501, 728
HgaI GACGC 4 cut(s) 290, 602, 1163, 1258
HhaI GCGC 1 cut(s) 270
Hin1I GRCGYC 3 cut(s) 282, 1155, 1269
Hin1II CATG 2 cut(s) 1118, 1145
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HincII GTYRAC 1 cut(s) 945
HindII GTYRAC 1 cut(s) 945
HindIII AAGCTT 1 cut(s) 1196
HinfI GANTC 7 cut(s) 71, 102, 300, 373, 767, 1027, 1049
HpaII CCGG 3 cut(s) 109, 501, 728
HphI GGTGA 2 cut(s) 134, 190
Hpy166II GTNNAC 5 cut(s) 64, 181, 945, 1120, 1238
Hpy188I TCNGA 7 cut(s) 191, 264, 299, 774, 901, 1104, 1126
Hpy188III TCNNGA 4 cut(s) 44, 501, 1042, 1254
Hpy8I GTNNAC 5 cut(s) 64, 181, 945, 1120, 1238
Hpy99I CGWCG 5 cut(s) 89, 275, 278, 905, 1321
HpyAV CCTTC 2 cut(s) 430, 902
HpyCH4III ACNGT 2 cut(s) 487, 1171
HpyCH4IV ACGT 3 cut(s) 599, 903, 1077
HpyCH4V TGCA 7 cut(s) 333, 521, 680, 721, 918, 1082, 1233
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 551, 785, 1195, 1227, 1239
HpyF3I CTNAG 3 cut(s) 296, 771, 1101
HpySE526I ACGT 3 cut(s) 599, 903, 1077
Hsp92I GRCGYC 3 cut(s) 282, 1155, 1269
Hsp92II CATG 2 cut(s) 1118, 1145
HspAI GCGC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 500
Kzo9I GATC 5 cut(s) 186, 643, 949, 1204, 1285
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 7 cut(s) 210, 505, 541, 775, 817, 1217, 1315
MaeI CTAG 1 cut(s) 930
MaeII ACGT 3 cut(s) 599, 903, 1077
MaeIII GTNAC 3 cut(s) 140, 614, 781
MalI GATC 5 cut(s) 188, 645, 951, 1206, 1287
MbiI CCGCTC 1 cut(s) 1323
MboI GATC 5 cut(s) 186, 643, 949, 1204, 1285
MboII GAAGA 6 cut(s) 35, 148, 382, 995, 1050, 1327
MflI RGATCY 2 cut(s) 949, 1204
MhlI GDGCHC 3 cut(s) 198, 390, 515
MlsI TGGCCA 3 cut(s) 233, 665, 752
MluNI TGGCCA 3 cut(s) 233, 665, 752
MlyI GAGTC 2 cut(s) 65, 1036
MmeI TCCRAC 1 cut(s) 924
Mox20I TGGCCA 3 cut(s) 233, 665, 752
MroI TCCGGA 1 cut(s) 500
MscI TGGCCA 3 cut(s) 233, 665, 752
MseI TTAA 2 cut(s) 696, 977
Msp20I TGGCCA 3 cut(s) 233, 665, 752
MspA1I CMGCKG 2 cut(s) 998, 1230
MspI CCGG 3 cut(s) 109, 501, 728
MspR9I CCNGG 2 cut(s) 171, 356
MvaI CCWGG 2 cut(s) 171, 356
MvnI CGCG 2 cut(s) 270, 1255
MwoI GCNNNNNNNGC 6 cut(s) 11, 551, 785, 1195, 1227, 1239
NdeII GATC 5 cut(s) 186, 643, 949, 1204, 1285
NlaIII CATG 2 cut(s) 1118, 1145
NlaIV GGNNCC 1 cut(s) 499
NmeAIII GCCGAG 2 cut(s) 310, 1269
NmuCI GTSAC 2 cut(s) 140, 614
NruI TCGCGA 1 cut(s) 1255
NspI RCATGY 1 cut(s) 1145
PcsI WCGNNNNNNNCGW 2 cut(s) 224, 282
PfeI GAWTC 5 cut(s) 102, 300, 373, 767, 1049
PflFI GACNNNGTC 1 cut(s) 1157
PkrI GCNGC 7 cut(s) 225, 520, 556, 790, 832, 1232, 1330
PleI GAGTC 2 cut(s) 65, 1035
PpsI GAGTC 2 cut(s) 65, 1035
Ppu21I YACGTR 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 515
Psp1406I AACGTT 1 cut(s) 1077
Psp6I CCWGG 2 cut(s) 169, 354
PspEI GGTNACC 1 cut(s) 140
PspFI CCCAGC 2 cut(s) 93, 998
PspGI CCWGG 2 cut(s) 169, 354
PspN4I GGNNCC 1 cut(s) 499
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PstI CTGCAG 1 cut(s) 1235
PstNI CAGNNNCTG 2 cut(s) 785, 953
PsuI RGATCY 2 cut(s) 949, 1204
PsyI GACNNNGTC 1 cut(s) 1157
PvuII CAGCTG 2 cut(s) 998, 1230
RruI TCGCGA 1 cut(s) 1255
RsaI GTAC 5 cut(s) 323, 407, 934, 1121, 1140
RsaNI GTAC 5 cut(s) 322, 406, 933, 1120, 1139
SacI GAGCTC 1 cut(s) 515
SaqAI TTAA 2 cut(s) 696, 977
SatI GCNGC 7 cut(s) 224, 519, 555, 789, 831, 1231, 1329
Sau3AI GATC 5 cut(s) 186, 643, 949, 1204, 1285
ScaI AGTACT 1 cut(s) 934
SchI GAGTC 2 cut(s) 65, 1036
ScrFI CCNGG 2 cut(s) 171, 356
SduI GDGCHC 3 cut(s) 198, 390, 515
SfcI CTRYAG 3 cut(s) 492, 1225, 1231
SsiI CCGC 4 cut(s) 342, 866, 1135, 1323
SspMI CTAG 1 cut(s) 930
SstI GAGCTC 1 cut(s) 515
StyD4I CCNGG 2 cut(s) 169, 354
TaaI ACNGT 2 cut(s) 487, 1171
TaiI ACGT 3 cut(s) 602, 906, 1080
TaqI TCGA 4 cut(s) 84, 434, 509, 1319
TaqII GACCGA 1 cut(s) 128
TatI WGTACW 2 cut(s) 932, 1119
TfiI GAWTC 5 cut(s) 102, 300, 373, 767, 1049
Tru1I TTAA 2 cut(s) 696, 977
Tru9I TTAA 2 cut(s) 696, 977
TscAI CASTG 1 cut(s) 1240
TseFI GTSAC 2 cut(s) 140, 614
TseI GCWGC 7 cut(s) 223, 518, 554, 788, 830, 1230, 1328
Tsp45I GTSAC 2 cut(s) 140, 614
TspDTI ATGAA 3 cut(s) 385, 875, 1226
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 1 cut(s) 1240
Tth111I GACNNNGTC 1 cut(s) 1157
XapI RAATTY 5 cut(s) 327, 422, 568, 973, 1014
XceI RCATGY 1 cut(s) 1145
XmiI GTMKAC 1 cut(s) 63
XspI CTAG 1 cut(s) 930
ZrmI AGTACT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.