Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0003653

Physical Location & Seq

Genomic Coordinates Reverse (-)

17826566 .. 17833893

Gene Structure 7328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGGCTGGATCGTCTTGGCCTTCTGAAGCTCAGCTAAATGGAATTAGACAGATAGTTTCAGAAATGGCTGGGACAGGTAGTGAAGAAGTTCGTGTCGTAGTTTCTCCTTATCGAATTTGTCCATTGGGAGCTCATATTGATCATCAGGGCGGGACTGTATCAGCTATGACAATTAACAAGGGAATACTTTTAGGTTTTGTTCCTTCTGGTGACACCCAGGTTATACTGCGTTCAGGACAGTTTAAGGGTGAATGTCGGTTCAGAGTTGATGAAGTTCAGTGCCCTTGGAAGTATGCAGCTGATGCTTCCAAAATAGATGAAGAAAGTGATTGGGGAAGTTATGCGAGAGGAGCCTTATATGCATTACAGAGTAGGCAGAACTCCCTTGCCCAGGGTATCATTGGATATATAATTGGTCCTGAAGGTATGGACAGTTCAGGCCTTAGCTCTTCTGCTGCTGTCGGTGTAGCTTACCTATTGGCTTTGGAAACTGCAAATAATCTAACAGTGTCCCCTGAAGAAAATATTGAGTATGATCGGTTGATTGAAAATGAATTTTGGGGCCTGCGAAATGGTATATTGGATCAATCAGCCATATTGCTTTCGAGCTATGGTTGTCTATTGTGCATGAACTGCAAGACTAAAGAACATAATCTTATACACCCACCAAAACTGGGAGTGAACCACGAGACTGAGTGGCAGGAAGCATACAAAATATTATTGGCATTTTCAGGCTTGAAGCATAATTTGACTGTCCATCCTGGATATAATCATCGAGTTGTAGAGTGTCGAGAAGCTGCAACAGTTCTTTTGAATGCATCCGGAGGTGGTGCAGCAGAGCCGCTCCTATCCAATGTTGACCCAGAAGTTTATCAGACTCACAAGCACATATTAGAACCTAATCTATCCAAGAGAGCGGAGCATTATTTCTCAGAGAATATGCGGGTTAGCAAAGGACTCGAAGCTTGGGCTTCGGGCAGGTTCAAAGATTTTGGAATGCTCATTTCAGAATCTGGCCTAAGTTCTATTCAGAACTATGAATGCGGTTCTGAACCGCTGATACAACTGCGCGAGATCCTTCTAAGGGCTCCTGGTGTCTATGGAGCACGGTTCAGTGGTGCTGGATTTAGAGGTTGCTGCGTTGCATTAGTGGATACTGGTCATGCCGATGAAGCTGCATCATTTGTCAGGGAAGAGTATGACAAGGTCCAACCTGAGTTGGCAAGTCAGTTAAACCATGATACAGCGGTTATGATATGCGAGGCTGGTGATTGTGCCCGTGTTATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006063 GO:0006082 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019586 GO:0019752 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046396 GO:0046835 GO:0047912 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N GalKase_gal_bdg

Protein Analysis

430

Amino Acids

46.7

Weight (kDa)

5.24

Isoelectric Point (pI)

44.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GHMP_kinases_N	PF00288	144 - 201	6.5e-09	GHMP kinases N terminal domain
GHMP_kinases_C	PF08544	324 - 401	8.6e-10	GHMP kinases C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10700 AT3G10700 AT3G10700
fragaria_vesca	FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430 FvH4_4g06430
malus_domestica	MD13G1235800.v1.1 MD16G1240500.v1.1
prunus_persica	Prupe.1G067200_v2.0.a1 Prupe.1G067200_v2.0.a1
pyrus_communis	pycom13g20830 pycom16g20150
rosa_chinensis	RchiOBHm_Chr4g0398191
rosa_laevigata	RLG00000009380
rosa_multiflora	Rmu_sc0002378.1_g000004
rosa_roxburghii	Rroxscaffold_5G00343090
rosa_rugosa	Rorug04G0006700 Rorug04G0006700
rosa_samantha	Rh4AG083900 Rh4BG081400 Rh4CG091000 Rh4DG076400
rosa_wichuraiana	Rw4G006980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	969
AccBSI	CCGCTC	2 cut(s)	844, 917
AccII	CGCG	1 cut(s)	1071
AccIII	TCCGGA	1 cut(s)	821
AciI	CCGC	7 cut(s)	150, 842, 917, 943, 1044, 1055, 1247
AclWI	GGATC	3 cut(s)	16, 591, 1069
AcsI	RAATTY	2 cut(s)	114, 554
AcuI	CTGAAG	3 cut(s)	45, 441, 537
AfiI	CCNNNNNNNGG	3 cut(s)	391, 674, 1084
AgsI	TTSAA	4 cut(s)	548, 739, 814, 985
AjnI	CCWGG	4 cut(s)	216, 390, 760, 1090
Alw21I	GWGCWC	2 cut(s)	133, 1108
Alw26I	GTCTC	1 cut(s)	683
AlwI	GGATC	3 cut(s)	16, 591, 1069
AlwNI	CAGNNNCTG	1 cut(s)	1013
Aor13HI	TCCGGA	1 cut(s)	821
AoxI	GGCC	4 cut(s)	17, 439, 562, 1015
ApeKI	GCWGC	6 cut(s)	296, 455, 797, 833, 1137, 1175
ApoI	RAATTY	2 cut(s)	114, 554
Asp700I	GAANNNNTTC	1 cut(s)	87
AspLEI	GCGC	1 cut(s)	1071
AspS9I	GGNCC	3 cut(s)	416, 562, 1207
AsuHPI	GGTGA	3 cut(s)	221, 260, 1280
AvaII	GGWCC	2 cut(s)	416, 1207
BaeGI	GKGCMC	2 cut(s)	284, 1279
BaeI	ACNNNNGTAYC	2 cut(s)	1233, 1266
BanII	GRGCYC	2 cut(s)	133, 1090
BauI	CACGAG	1 cut(s)	686
Bbv12I	GWGCWC	2 cut(s)	133, 1108
BbvI	GCAGC	6 cut(s)	308, 442, 784, 845, 1124, 1162
BccI	CCATC	1 cut(s)	765
BcgI	CGANNNNNNTGC	4 cut(s)	940, 974, 1157, 1191
BciT130I	CCWGG	4 cut(s)	218, 392, 762, 1092
BciVI	GTATCC	1 cut(s)	1147
BclI	TGATCA	1 cut(s)	139
BcoDI	GTCTC	1 cut(s)	683
BfuAI	ACCTGC	1 cut(s)	969
BfuI	GTATCC	1 cut(s)	1147
BisI	GCNGC	7 cut(s)	297, 456, 798, 834, 842, 1138, 1176
BlpI	GCTNAGC	1 cut(s)	30
BlsI	GCNGC	7 cut(s)	298, 457, 799, 835, 843, 1139, 1177
Bme1390I	CCNGG	4 cut(s)	218, 392, 762, 1092
Bme18I	GGWCC	2 cut(s)	416, 1207
BmgT120I	GGNCC	3 cut(s)	416, 562, 1207
BmiI	GGNNCC	3 cut(s)	352, 563, 1089
BmrFI	CCNGG	4 cut(s)	218, 392, 762, 1092
BmrI	ACTGGG	1 cut(s)	683
BmsI	GCATC	3 cut(s)	292, 827, 1187
BmuI	ACTGGG	1 cut(s)	683
Bpu10I	CCTNAGC	1 cut(s)	443
Bpu1102I	GCTNAGC	1 cut(s)	30
BsaJI	CCNNGG	4 cut(s)	216, 284, 390, 391
BsaWI	WCCGGW	1 cut(s)	821
Bsc4I	CCNNNNNNNGG	3 cut(s)	391, 674, 1084
Bse1I	ACTGG	2 cut(s)	678, 1162
BseAI	TCCGGA	1 cut(s)	821
BseBI	CCWGG	4 cut(s)	218, 392, 762, 1092
BseDI	CCNNGG	4 cut(s)	216, 284, 390, 391
BseGI	GGATG	2 cut(s)	757, 818
BseLI	CCNNNNNNNGG	3 cut(s)	391, 674, 1084
BseMII	CTCAG	4 cut(s)	44, 684, 945, 1206
BseNI	ACTGG	2 cut(s)	678, 1162
BseRI	GAGGAG	1 cut(s)	363
BseSI	GKGCMC	2 cut(s)	284, 1279
BseXI	GCAGC	6 cut(s)	308, 442, 784, 845, 1124, 1162
BseYI	CCCAGC	1 cut(s)	68
BsgI	GTGCAG	1 cut(s)	852
Bsh1236I	CGCG	1 cut(s)	1071
BshFI	GGCC	4 cut(s)	19, 441, 564, 1017
BsiHKAI	GWGCWC	2 cut(s)	133, 1108
BsiSI	CCGG	1 cut(s)	822
BslFI	GGGAC	3 cut(s)	85, 166, 496
BslI	CCNNNNNNNGG	3 cut(s)	391, 674, 1084
BsmAI	GTCTC	1 cut(s)	683
BsmFI	GGGAC	3 cut(s)	85, 166, 496
BsmI	GAATGC	3 cut(s)	820, 1002, 1046
BsnI	GGCC	4 cut(s)	19, 441, 564, 1017
Bsp1286I	GDGCHC	5 cut(s)	133, 284, 1090, 1108, 1279
Bsp13I	TCCGGA	1 cut(s)	821
Bsp143I	GATC	5 cut(s)	8, 139, 535, 583, 1074
Bsp1720I	GCTNAGC	1 cut(s)	30
BspACI	CCGC	7 cut(s)	150, 842, 917, 943, 1044, 1055, 1247
BspANI	GGCC	4 cut(s)	19, 441, 564, 1017
BspCNI	CTCAG	4 cut(s)	43, 685, 944, 1207
BspEI	TCCGGA	1 cut(s)	821
BspFNI	CGCG	1 cut(s)	1071
BspLI	GGNNCC	3 cut(s)	352, 563, 1089
BspMI	ACCTGC	1 cut(s)	969
BspPI	GGATC	3 cut(s)	16, 591, 1069
BspQI	GCTCTTC	1 cut(s)	454
BsrBI	CCGCTC	2 cut(s)	844, 917
BsrI	ACTGG	2 cut(s)	678, 1162
BssECI	CCNNGG	4 cut(s)	216, 284, 390, 391
BssMI	GATC	5 cut(s)	8, 139, 535, 583, 1074
BssSI	CACGAG	1 cut(s)	686
BssT1I	CCWWGG	1 cut(s)	284
Bst2BI	CACGAG	1 cut(s)	686
Bst2UI	CCWGG	4 cut(s)	218, 392, 762, 1092
Bst4CI	ACNGT	7 cut(s)	157, 240, 434, 508, 754, 805, 1110
Bst6I	CTCTTC	2 cut(s)	454, 1188
BstAPI	GCANNNNNTGC	2 cut(s)	302, 633
BstC8I	GCNNGC	1 cut(s)	566
BstDEI	CTNAG	7 cut(s)	30, 443, 693, 931, 1019, 1082, 1215
BstENI	CCTNNNNNAGG	1 cut(s)	389
BstF5I	GGATG	2 cut(s)	757, 818
BstFNI	CGCG	1 cut(s)	1071
BstHHI	GCGC	1 cut(s)	1071
BstKTI	GATC	5 cut(s)	11, 142, 538, 586, 1077
BstMAI	GTCTC	1 cut(s)	683
BstMBI	GATC	5 cut(s)	8, 139, 535, 583, 1074
BstMWI	GCNNNNNNNGC	5 cut(s)	302, 350, 359, 633, 1172
BstNI	CCWGG	4 cut(s)	218, 392, 762, 1092
BstSCI	CCNGG	4 cut(s)	216, 390, 760, 1090
BstSLI	GKGCMC	2 cut(s)	284, 1279
BstUI	CGCG	1 cut(s)	1071
BstV1I	GCAGC	6 cut(s)	308, 442, 784, 845, 1124, 1162
BstX2I	RGATCY	1 cut(s)	1074
BstYI	RGATCY	1 cut(s)	1074
BsuI	GTATCC	1 cut(s)	1147
BsuRI	GGCC	4 cut(s)	19, 441, 564, 1017
BtsCI	GGATG	2 cut(s)	757, 818
BtsIMutI	CAGTG	3 cut(s)	284, 513, 1120
BveI	ACCTGC	1 cut(s)	969
Cac8I	GCNNGC	1 cut(s)	566
CaiI	CAGNNNCTG	1 cut(s)	1013
CfoI	GCGC	1 cut(s)	1071
Cfr13I	GGNCC	3 cut(s)	416, 562, 1207
CviAII	CATG	3 cut(s)	628, 1163, 1238
DdeI	CTNAG	7 cut(s)	30, 443, 693, 931, 1019, 1082, 1215
DpnI	GATC	5 cut(s)	10, 141, 537, 585, 1076
DpnII	GATC	5 cut(s)	8, 139, 535, 583, 1074
Eam1104I	CTCTTC	2 cut(s)	454, 1188
EarI	CTCTTC	2 cut(s)	454, 1188
Ecl136II	GAGCTC	1 cut(s)	131
Eco130I	CCWWGG	1 cut(s)	284
Eco147I	AGGCCT	1 cut(s)	441
Eco24I	GRGCYC	2 cut(s)	133, 1090
Eco47I	GGWCC	2 cut(s)	416, 1207
Eco53kI	GAGCTC	1 cut(s)	131
Eco57I	CTGAAG	3 cut(s)	45, 441, 537
EcoICRI	GAGCTC	1 cut(s)	131
EcoNI	CCTNNNNNAGG	1 cut(s)	389
EcoO109I	RGGNCCY	1 cut(s)	562
EcoRII	CCWGG	4 cut(s)	216, 390, 760, 1090
EcoT14I	CCWWGG	1 cut(s)	284
EcoT22I	ATGCAT	2 cut(s)	364, 820
EcoT38I	GRGCYC	2 cut(s)	133, 1090
ErhI	CCWWGG	1 cut(s)	284
FaeI	CATG	3 cut(s)	631, 1166, 1241
FaqI	GGGAC	3 cut(s)	85, 166, 496
FatI	CATG	3 cut(s)	627, 1162, 1237
FauI	CCCGC	2 cut(s)	143, 936
FbaI	TGATCA	1 cut(s)	139
Fnu4HI	GCNGC	7 cut(s)	297, 456, 798, 834, 842, 1138, 1176
FokI	GGATG	2 cut(s)	744, 805
FriOI	GRGCYC	2 cut(s)	133, 1090
Fsp4HI	GCNGC	7 cut(s)	297, 456, 798, 834, 842, 1138, 1176
GlaI	GCGC	1 cut(s)	1070
GluI	GCNGC	7 cut(s)	297, 456, 798, 834, 842, 1138, 1176
GsaI	CCCAGC	1 cut(s)	72
HaeIII	GGCC	4 cut(s)	19, 441, 564, 1017
HapII	CCGG	1 cut(s)	822
HhaI	GCGC	1 cut(s)	1071
Hin1II	CATG	3 cut(s)	631, 1166, 1241
Hin6I	GCGC	1 cut(s)	1069
HinP1I	GCGC	1 cut(s)	1069
HincII	GTYRAC	1 cut(s)	859
HindII	GTYRAC	1 cut(s)	859
HindIII	AAGCTT	1 cut(s)	963
HinfI	GANTC	3 cut(s)	877, 957, 1010
HpaII	CCGG	1 cut(s)	822
HphI	GGTGA	3 cut(s)	221, 260, 1280
Hpy166II	GTNNAC	2 cut(s)	682, 859
Hpy188I	TCNGA	8 cut(s)	25, 61, 263, 876, 934, 1009, 1032, 1051
Hpy188III	TCNNGA	4 cut(s)	234, 419, 791, 822
Hpy8I	GTNNAC	2 cut(s)	682, 859
HpyAV	CCTTC	4 cut(s)	30, 213, 416, 1088
HpyCH4III	ACNGT	7 cut(s)	157, 240, 434, 508, 754, 805, 1110
HpyF10VI	GCNNNNNNNGC	5 cut(s)	302, 350, 359, 633, 1172
HpyF3I	CTNAG	7 cut(s)	30, 443, 693, 931, 1019, 1082, 1215
Hsp92II	CATG	3 cut(s)	631, 1166, 1241
HspAI	GCGC	1 cut(s)	1069
Kpn2I	TCCGGA	1 cut(s)	821
Ksp22I	TGATCA	1 cut(s)	139
Kzo9I	GATC	5 cut(s)	8, 139, 535, 583, 1074
LguI	GCTCTTC	1 cut(s)	454
LmnI	GCTCC	6 cut(s)	128, 350, 849, 919, 1093, 1103
Lsp1109I	GCAGC	6 cut(s)	308, 442, 784, 845, 1124, 1162
LweI	GCATC	3 cut(s)	292, 827, 1187
MaeIII	GTNAC	1 cut(s)	209
MalI	GATC	5 cut(s)	10, 141, 537, 585, 1076
MbiI	CCGCTC	2 cut(s)	844, 917
MboI	GATC	5 cut(s)	8, 139, 535, 583, 1074
MboII	GAAGA	5 cut(s)	95, 332, 441, 530, 1205
MflI	RGATCY	1 cut(s)	1074
MhlI	GDGCHC	5 cut(s)	133, 284, 1090, 1108, 1279
MluCI	AATT	6 cut(s)	42, 114, 171, 411, 554, 745
MlyI	GAGTC	2 cut(s)	871, 951
MmeI	TCCRAC	1 cut(s)	1234
MnlI	CCTC	4 cut(s)	341, 818, 1124, 1255
Mph1103I	ATGCAT	2 cut(s)	364, 820
MroI	TCCGGA	1 cut(s)	821
MroXI	GAANNNNTTC	1 cut(s)	87
MseI	TTAA	3 cut(s)	174, 243, 1232
MslI	CAYNNNNRTG	1 cut(s)	1167
MspA1I	CMGCKG	3 cut(s)	299, 1057, 1247
MspI	CCGG	1 cut(s)	822
MspR9I	CCNGG	4 cut(s)	218, 392, 762, 1092
Mva1269I	GAATGC	3 cut(s)	820, 1002, 1046
MvaI	CCWGG	4 cut(s)	218, 392, 762, 1092
MvnI	CGCG	1 cut(s)	1071
MwoI	GCNNNNNNNGC	5 cut(s)	302, 350, 359, 633, 1172
NdeII	GATC	5 cut(s)	8, 139, 535, 583, 1074
NlaIII	CATG	3 cut(s)	631, 1166, 1241
NlaIV	GGNNCC	3 cut(s)	352, 563, 1089
NmuCI	GTSAC	1 cut(s)	209
NsiI	ATGCAT	2 cut(s)	364, 820
PasI	CCCWGGG	1 cut(s)	391
PceI	AGGCCT	1 cut(s)	441
PciSI	GCTCTTC	1 cut(s)	454
PctI	GAATGC	3 cut(s)	820, 1002, 1046
PdmI	GAANNNNTTC	1 cut(s)	87
PfeI	GAWTC	1 cut(s)	1010
PflFI	GACNNNGTC	1 cut(s)	1205
PfoI	TCCNGGA	1 cut(s)	760
PkrI	GCNGC	7 cut(s)	298, 457, 799, 835, 843, 1139, 1177
PleI	GAGTC	2 cut(s)	871, 951
PpsI	GAGTC	2 cut(s)	871, 951
Psp124BI	GAGCTC	1 cut(s)	133
Psp6I	CCWGG	4 cut(s)	216, 390, 760, 1090
PspFI	CCCAGC	1 cut(s)	68
PspGI	CCWGG	4 cut(s)	216, 390, 760, 1090
PspN4I	GGNNCC	3 cut(s)	352, 563, 1089
PspPI	GGNCC	3 cut(s)	416, 562, 1207
PsrI	GAACNNNNNNTAC	4 cut(s)	418, 450, 1044, 1076
PstNI	CAGNNNCTG	1 cut(s)	1013
PsuI	RGATCY	1 cut(s)	1074
PsyI	GACNNNGTC	1 cut(s)	1205
PvuII	CAGCTG	1 cut(s)	299
RseI	CAYNNNNRTG	1 cut(s)	1167
SacI	GAGCTC	1 cut(s)	133
SapI	GCTCTTC	1 cut(s)	454
SaqAI	TTAA	3 cut(s)	174, 243, 1232
SatI	GCNGC	7 cut(s)	297, 456, 798, 834, 842, 1138, 1176
Sau3AI	GATC	5 cut(s)	8, 139, 535, 583, 1074
Sau96I	GGNCC	3 cut(s)	416, 562, 1207
SchI	GAGTC	2 cut(s)	871, 951
ScrFI	CCNGG	4 cut(s)	218, 392, 762, 1092
SduI	GDGCHC	5 cut(s)	133, 284, 1090, 1108, 1279
SfaNI	GCATC	3 cut(s)	292, 827, 1187
SinI	GGWCC	2 cut(s)	416, 1207
SmiMI	CAYNNNNRTG	1 cut(s)	1167
Sse9I	AATT	6 cut(s)	42, 114, 171, 411, 554, 745
SseBI	AGGCCT	1 cut(s)	441
SsiI	CCGC	7 cut(s)	150, 842, 917, 943, 1044, 1055, 1247
SspI	AATATT	2 cut(s)	526, 717
SstI	GAGCTC	1 cut(s)	133
StuI	AGGCCT	1 cut(s)	441
StyD4I	CCNGG	4 cut(s)	216, 390, 760, 1090
StyI	CCWWGG	1 cut(s)	284
TaaI	ACNGT	7 cut(s)	157, 240, 434, 508, 754, 805, 1110
TaqI	TCGA	5 cut(s)	112, 605, 775, 790, 960
TasI	AATT	6 cut(s)	42, 114, 171, 411, 554, 745
TauI	GCSGC	1 cut(s)	844
TfiI	GAWTC	1 cut(s)	1010
Tru1I	TTAA	3 cut(s)	174, 243, 1232
Tru9I	TTAA	3 cut(s)	174, 243, 1232
TscAI	CASTG	3 cut(s)	284, 513, 1120
TseFI	GTSAC	1 cut(s)	209
TseI	GCWGC	6 cut(s)	296, 455, 797, 833, 1137, 1175
Tsp45I	GTSAC	1 cut(s)	209
TspDTI	ATGAA	6 cut(s)	285, 333, 567, 644, 1053, 1185
TspRI	CASTG	3 cut(s)	284, 513, 1120
Tth111I	GACNNNGTC	1 cut(s)	1205
VpaK11BI	GGWCC	2 cut(s)	416, 1207
XagI	CCTNNNNNAGG	1 cut(s)	389
XapI	RAATTY	2 cut(s)	114, 554
XcmI	CCANNNNNNNNNTGG	1 cut(s)	398
XmnI	GAANNNNTTC	1 cut(s)	87
Zsp2I	ATGCAT	2 cut(s)	364, 820

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.