pycom17g01420

F-box protein SKIP14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005923
Physical Location & Seq
Reverse (-)
959913 .. 961098
1186 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGATGCTGTGTGTTATGCACTGCGTTTTTCTTGGTGTTAAGGACCTTCTTTCGGTTGAAAGGGTTTGCAAGTCTTTTCGTAATGCAGTTAGAAGTGACCCTCTTCTATGGAGGAATATTATCATCGATTGGCCGTTGAATGAGCGCATCTTCGATGATGTTCTAGTAAAATTAACTAGCAGGGCTCAAGGCACACTTCAAACCTTGGGTCTTGTGCATTGCGTAAGGATCACTGACATTGGGCTGCAGGGTGTGTTTGACAGCAACCCGAGGTTGACAAAGTTAAGTGTTCCCGGATGTCTTAAAATCAGTGTTGAGTGCATCTTGTTAAACATAAGGACCTTGAAGTCTGCAGGCAACCCTGGAATAAAGCAGTTAAGAATTGCTGGTCTCTCTGGTATAACTGACAAGCAGTTCGAAGAGTTGAAATTCTTGCTTGGTGCAGATAACCACATTCAGCTTAGAGCCCCGAAACCTTGGTTTTTTAAAGGTGGGTTGTTGTATGGCTCTTGTGATGATGATTGTCCCATCGACATCGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

182

Amino Acids

20.21

Weight (kDa)

8.44

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 10 - 41 4.6e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 346
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 131
AcsI RAATTY 1 cut(s) 428
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 5 cut(s) 59, 139, 200, 346, 427
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 1 cut(s) 460
AluI AGCT 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 1 cut(s) 131
ApeKI GCWGC 1 cut(s) 244
ApoI RAATTY 1 cut(s) 428
ArsI GACNNNNNNTTYG 4 cut(s) 193, 225, 398, 430
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 43, 339
AsuC2I CCSGG 1 cut(s) 294
AsuII TTCGAA 1 cut(s) 417
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 2 cut(s) 43, 339
BanII GRGCYC 2 cut(s) 187, 469
BbvI GCAGC 1 cut(s) 231
BccI CCATC 1 cut(s) 536
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 363
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 164, 177
BfmI CTRYAG 2 cut(s) 245, 351
BisI GCNGC 1 cut(s) 245
BlsI GCNGC 1 cut(s) 246
Bme1390I CCNGG 2 cut(s) 294, 363
Bme18I GGWCC 2 cut(s) 43, 339
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 43, 339
BmrFI CCNGG 2 cut(s) 294, 363
BmsI GCATC 2 cut(s) 156, 330
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 4 cut(s) 204, 269, 361, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 217
BseBI CCWGG 1 cut(s) 363
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 4 cut(s) 204, 269, 361, 476
BseGI GGATG 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 462
BshFI GGCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 1 cut(s) 133
Bso31I GGTCTC 1 cut(s) 395
BsoBI CYCGRG 1 cut(s) 268
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 187, 469
Bsp143I GATC 1 cut(s) 228
BspANI GGCC 1 cut(s) 133
BspDI ATCGAT 1 cut(s) 126
BspMAI CTGCAG 2 cut(s) 249, 355
BspPI GGATC 1 cut(s) 236
BspT104I TTCGAA 1 cut(s) 417
BspTNI GGTCTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 217
BssECI CCNNGG 4 cut(s) 204, 269, 361, 476
BssMI GATC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 204, 476
Bst2UI CCWGG 1 cut(s) 363
Bst6I CTCTTC 2 cut(s) 108, 414
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 1 cut(s) 461
BstF5I GGATG 1 cut(s) 302
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 1 cut(s) 231
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 228
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 2 cut(s) 292, 361
BstSFI CTRYAG 2 cut(s) 245, 351
BstV1I GCAGC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 1 cut(s) 133
BsuTUI ATCGAT 1 cut(s) 126
BtsCI GGATG 1 cut(s) 302
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 3 cut(s) 19, 231, 316
Cac8I GCNNGC 1 cut(s) 355
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 2 cut(s) 43, 339
ClaI ATCGAT 1 cut(s) 126
CviAII CATG 1 cut(s) 543
CviJI RGCY 6 cut(s) 133, 185, 244, 460, 467, 507
CviKI_1 RGCY 6 cut(s) 133, 185, 244, 460, 467, 507
DdeI CTNAG 1 cut(s) 461
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
DraI TTTAAA 1 cut(s) 487
DrdI GACNNNNNNGTC 1 cut(s) 346
DseDI GACNNNNNNGTC 1 cut(s) 346
EaeI YGGCCR 1 cut(s) 131
Eam1104I CTCTTC 2 cut(s) 108, 414
EarI CTCTTC 2 cut(s) 108, 414
Eco130I CCWWGG 2 cut(s) 204, 476
Eco24I GRGCYC 2 cut(s) 187, 469
Eco31I GGTCTC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 43, 339
Eco88I CYCGRG 1 cut(s) 268
EcoO109I RGGNCCY 2 cut(s) 43, 339
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 2 cut(s) 204, 476
EcoT38I GRGCYC 2 cut(s) 187, 469
ErhI CCWWGG 2 cut(s) 204, 476
FaeI CATG 1 cut(s) 546
FaiI YATR 6 cut(s) 17, 109, 335, 401, 504, 544
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 1 cut(s) 510
FatI CATG 1 cut(s) 542
Fnu4HI GCNGC 1 cut(s) 245
FokI GGATG 1 cut(s) 309
FriOI GRGCYC 2 cut(s) 187, 469
Fsp4HI GCNGC 1 cut(s) 245
FspBI CTAG 2 cut(s) 164, 177
GlaI GCGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 245
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 147
Hin1II CATG 1 cut(s) 546
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HpaII CCGG 1 cut(s) 294
Hpy166II GTNNAC 1 cut(s) 276
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 1 cut(s) 56
HpyCH4V TGCA 8 cut(s) 19, 69, 86, 217, 247, 321, 353, 443
HpyF3I CTNAG 1 cut(s) 461
Hsp92II CATG 1 cut(s) 546
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 1 cut(s) 228
LpnPI CCDG 8 cut(s) 166, 233, 307, 339, 348, 372, 375, 381
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 2 cut(s) 156, 330
MaeI CTAG 2 cut(s) 164, 177
MaeIII GTNAC 1 cut(s) 95
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 3 cut(s) 95, 142, 431
MhlI GDGCHC 2 cut(s) 187, 469
MluCI AATT 3 cut(s) 170, 381, 428
MnlI CCTC 3 cut(s) 105, 111, 264
MseI TTAA 7 cut(s) 39, 173, 284, 303, 329, 377, 486
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 294, 363
MvaI CCWGG 1 cut(s) 363
NciI CCSGG 1 cut(s) 294
NdeII GATC 1 cut(s) 228
NlaIII CATG 1 cut(s) 546
NmuCI GTSAC 1 cut(s) 95
NspV TTCGAA 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 1 cut(s) 246
PpuMI RGGWCCY 2 cut(s) 43, 339
Psp5II RGGWCCY 2 cut(s) 43, 339
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspPI GGNCC 2 cut(s) 43, 339
PspPPI RGGWCCY 2 cut(s) 43, 339
PstI CTGCAG 2 cut(s) 249, 355
SaqAI TTAA 7 cut(s) 39, 173, 284, 303, 329, 377, 486
SatI GCNGC 1 cut(s) 245
Sau3AI GATC 1 cut(s) 228
Sau96I GGNCC 2 cut(s) 43, 339
ScrFI CCNGG 2 cut(s) 294, 363
SduI GDGCHC 2 cut(s) 187, 469
SetI ASST 7 cut(s) 48, 206, 275, 344, 462, 478, 493
SfaNI GCATC 2 cut(s) 156, 330
SfcI CTRYAG 2 cut(s) 245, 351
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 2 cut(s) 43, 339
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
Sse9I AATT 3 cut(s) 170, 381, 428
SspI AATATT 1 cut(s) 118
SspMI CTAG 2 cut(s) 164, 177
StyD4I CCNGG 2 cut(s) 292, 361
StyI CCWWGG 2 cut(s) 204, 476
TaqI TCGA 5 cut(s) 126, 153, 417, 531, 537
TasI AATT 3 cut(s) 170, 381, 428
Tru1I TTAA 7 cut(s) 39, 173, 284, 303, 329, 377, 486
Tru9I TTAA 7 cut(s) 39, 173, 284, 303, 329, 377, 486
TscAI CASTG 3 cut(s) 26, 238, 316
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 244
Tsp45I GTSAC 1 cut(s) 95
TspRI CASTG 3 cut(s) 26, 238, 316
VpaK11BI GGWCC 2 cut(s) 43, 339
XapI RAATTY 1 cut(s) 428
XspI CTAG 2 cut(s) 164, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.