pycom17g04290

Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0014150
Physical Location & Seq
Reverse (-)
2927135 .. 2930158
3024 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ATGTACGGCTTCGAAGCGATGACCTTCAACATTCACGGCGGGTACTTGGAGGCCATAGTGCGGGGACACCGCTCCGGCCTCCTCACCGCCGCCGATTACAACAATTTGTGCCAGTGCGAGACCCTCGACGATATCAAGATGCACCTTTCTGCCACTGAGTACGGCCCCTACCTCCAAAACGAACCTTCCCCATTGCATACAACTACAATTGTGGAGAAGTGCACTCTTAAATTGGTTGATGAGTATAAGCACATGCTATGCCAAGCTACAGAGCCCTTGTCAACCTTTTTGGAGTATATTACATATGGTCACATGATAGACAATGTTGTCCTGATTGTTACTGGAACCTTGCATGAGAGAGATGTTCAGGAACTGTTGGAAAAATGCCACCCCTTGGGCATGTTTGACAGCATTGCCACCCTGGCGGTTGCACAGAATATGCGGGAGCTTTACAGATTGGTGCTTGTTGACACACCACTTGCTCCCTACTTTTCTGAATGTATTACATCCGATGACCTGGATGACATGAACATTGAAATAATGAGGAACACTCTTTACAAGGCATACCTTGAAGACTTTTACAGGTTTTGTCAGAAACTAGGTGGTGCCACAGCAGAGATTATGTCTGACTTACTTGCTTTTGAGGCTGACAGAAGGGCTGTCAATATTACAATAAACAGTATCGGAACTGAGCTTACTCGAGACGATCGCTGGAAGTTGTACTCTAGCTTTGGTTTACTCTATCCCTATGGCCACGAGGAACTTGCTGTTTGTGAGGACATTGATCAGGTTCGTGGTTGTATGGAAAAATATCCCCCTTATCAGTCGATTTTTTCAAAACTATCCTATGGTGAGAGCCAGCTACTGGACAAGGCATTTTATGAAGAGGAGGTGAAAAGGCTTTGCCTGGCATTTGAGCAACAGTTCCATTACGGTGTTTTCTTTGCATACATGAGGTTGAGGGAGCAGGAGATCAGAAATCTGATGTGGATATCGGAGTGTGTGGCGCAGAACCAGAAGTCCCGAGTTCACGACAGCGTGGTCTTCATATTTTAG

Protein Analysis

352

Amino Acids

40.64

Weight (kDa)

4.85

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_AC39 PF01992 16 - 344 1.2e-107 ATP synthase (C/AC39) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28710 AT3G28715 AT3G28715
fragaria_vesca FvH4_6g49160
malus_domestica MD09G1048000.v1.1 MD17G1048200.v1.1
prunus_persica Prupe.3G271000_v2.0.a1 Prupe.3G271000_v2.0.a1
pyrus_communis pycom17g04290
rosa_chinensis RchiOBHm_Chr2g0169031
rosa_laevigata RLG00000021825
rosa_multiflora Rmu_ssc0000393.1_g000024
rosa_roxburghii Rroxscaffold_2G00082520
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG607900 Rh2CG589500
rosa_wichuraiana Rw2G050410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 326, 1040
AccB1I GGYRCC 1 cut(s) 605
AccB7I CCANNNNNTGG 2 cut(s) 394, 865
AccBSI CCGCTC 1 cut(s) 72
AciI CCGC 7 cut(s) 39, 61, 70, 87, 90, 425, 442
AcoI YGGCCR 1 cut(s) 751
AfaI GTAC 4 cut(s) 5, 44, 161, 722
AfiI CCNNNNNNNGG 3 cut(s) 60, 394, 865
AgsI TTSAA 4 cut(s) 28, 536, 572, 837
AjnI CCWGG 3 cut(s) 420, 516, 906
AloI GAACNNNNNNTCC 2 cut(s) 1004, 1036
AluBI AGCT 5 cut(s) 266, 448, 694, 729, 862
AluI AGCT 5 cut(s) 266, 448, 694, 729, 862
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 2 cut(s) 113, 696
Alw44I GTGCAC 1 cut(s) 220
AlwNI CAGNNNCTG 2 cut(s) 373, 865
Ama87I CYCGRG 2 cut(s) 699, 1023
AoxI GGCC 4 cut(s) 51, 76, 163, 751
ApaLI GTGCAC 1 cut(s) 220
AspLEI GCGC 1 cut(s) 1009
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 3 cut(s) 76, 863, 904
AsuII TTCGAA 1 cut(s) 12
AvaI CYCGRG 2 cut(s) 699, 1023
BaeGI GKGCMC 1 cut(s) 224
BalI TGGCCA 1 cut(s) 753
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 276
BauI CACGAG 1 cut(s) 755
BbsI GAAGAC 2 cut(s) 579, 1036
Bbv12I GWGCWC 1 cut(s) 224
BceAI ACGGC 3 cut(s) 22, 52, 178
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BciT130I CCWGG 3 cut(s) 422, 518, 908
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 2 cut(s) 113, 696
BfaI CTAG 2 cut(s) 599, 726
BfmI CTRYAG 1 cut(s) 267
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 3 cut(s) 422, 518, 908
BmeT110I CYCGRG 2 cut(s) 699, 1023
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 3 cut(s) 166, 346, 607
BmrFI CCNGG 3 cut(s) 422, 518, 908
BmsI GCATC 1 cut(s) 129
BpiI GAAGAC 2 cut(s) 579, 1036
BplI GAGNNNNNCTC 2 cut(s) 535, 567
Bpu14I TTCGAA 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 393, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 394, 865
Bse1I ACTGG 3 cut(s) 112, 346, 870
Bse3DI GCAATG 2 cut(s) 191, 411
BseBI CCWGG 3 cut(s) 422, 518, 908
BseDI CCNNGG 2 cut(s) 393, 420
BseGI GGATG 2 cut(s) 506, 526
BseLI CCNNNNNNNGG 3 cut(s) 60, 394, 865
BseMI GCAATG 2 cut(s) 191, 411
BseMII CTCAG 2 cut(s) 147, 681
BseNI ACTGG 3 cut(s) 112, 346, 870
BseRI GAGGAG 2 cut(s) 71, 902
BseSI GKGCMC 1 cut(s) 224
Bsh1285I CGRYCG 1 cut(s) 709
BshFI GGCC 4 cut(s) 53, 78, 165, 753
BshNI GGYRCC 1 cut(s) 605
BsiEI CGRYCG 1 cut(s) 709
BsiHKAI GWGCWC 1 cut(s) 224
BsiHKCI CYCGRG 2 cut(s) 699, 1023
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 2 cut(s) 78, 1006
BslI CCNNNNNNNGG 3 cut(s) 60, 394, 865
BsmAI GTCTC 2 cut(s) 113, 696
BsmBI CGTCTC 1 cut(s) 696
BsmFI GGGAC 2 cut(s) 78, 1006
BsnI GGCC 4 cut(s) 53, 78, 165, 753
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 2 cut(s) 699, 1023
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 224, 276
Bsp143I GATC 3 cut(s) 706, 784, 972
BspACI CCGC 7 cut(s) 39, 61, 70, 87, 90, 425, 442
BspANI GGCC 4 cut(s) 53, 78, 165, 753
BspCNI CTCAG 2 cut(s) 148, 682
BspLI GGNNCC 3 cut(s) 166, 346, 607
BspT104I TTCGAA 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 191, 411
BsrI ACTGG 3 cut(s) 112, 346, 870
BssECI CCNNGG 2 cut(s) 393, 420
BssMI GATC 3 cut(s) 706, 784, 972
BssSI CACGAG 1 cut(s) 755
BssT1I CCWWGG 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 3 cut(s) 422, 518, 908
Bst4CI ACNGT 4 cut(s) 375, 680, 924, 935
Bst6I CTCTTC 1 cut(s) 879
BstBI TTCGAA 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 860
BstDEI CTNAG 2 cut(s) 156, 690
BstF5I GGATG 2 cut(s) 506, 526
BstHHI GCGC 1 cut(s) 1009
BstKTI GATC 3 cut(s) 709, 787, 975
BstMAI GTCTC 2 cut(s) 113, 696
BstMBI GATC 3 cut(s) 706, 784, 972
BstMCI CGRYCG 1 cut(s) 709
BstMWI GCNNNNNNNGC 2 cut(s) 422, 644
BstNI CCWGG 3 cut(s) 422, 518, 908
BstNSI RCATGY 2 cut(s) 256, 403
BstSCI CCNGG 3 cut(s) 420, 516, 906
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 224
BstV2I GAAGAC 2 cut(s) 579, 1036
BsuRI GGCC 4 cut(s) 53, 78, 165, 753
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 2 cut(s) 506, 526
BtsIMutI CAGTG 2 cut(s) 119, 153
Cac8I GCNNGC 1 cut(s) 860
CaiI CAGNNNCTG 2 cut(s) 373, 865
CfoI GCGC 1 cut(s) 1009
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 4 cut(s) 4, 43, 160, 721
CviAII CATG 6 cut(s) 253, 313, 353, 400, 526, 952
CviQI GTAC 4 cut(s) 4, 43, 160, 721
DdeI CTNAG 2 cut(s) 156, 690
DpnI GATC 3 cut(s) 708, 786, 974
DpnII GATC 3 cut(s) 706, 784, 972
DrdI GACNNNNNNGTC 2 cut(s) 326, 1040
DseDI GACNNNNNNGTC 2 cut(s) 326, 1040
EaeI YGGCCR 1 cut(s) 751
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 113
Eco32I GATATC 2 cut(s) 133, 993
Eco88I CYCGRG 2 cut(s) 699, 1023
EcoRII CCWGG 3 cut(s) 420, 516, 906
EcoRV GATATC 2 cut(s) 133, 993
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 1 cut(s) 696
FaeI CATG 6 cut(s) 256, 316, 356, 403, 529, 955
FaqI GGGAC 2 cut(s) 78, 1006
FatI CATG 6 cut(s) 252, 312, 352, 399, 525, 951
FauI CCCGC 3 cut(s) 32, 54, 435
FauNDI CATATG 1 cut(s) 304
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 2 cut(s) 493, 533
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 90
FspBI CTAG 2 cut(s) 599, 726
GlaI GCGC 1 cut(s) 1008
GluI GCNGC 1 cut(s) 90
HaeIII GGCC 4 cut(s) 53, 78, 165, 753
HapII CCGG 1 cut(s) 75
HhaI GCGC 1 cut(s) 1009
Hin1II CATG 6 cut(s) 256, 316, 356, 403, 529, 955
Hin6I GCGC 1 cut(s) 1007
HinP1I GCGC 1 cut(s) 1007
HincII GTYRAC 2 cut(s) 282, 469
HindII GTYRAC 2 cut(s) 282, 469
HpaII CCGG 1 cut(s) 75
HphI GGTGA 3 cut(s) 76, 863, 904
Hpy166II GTNNAC 5 cut(s) 222, 282, 469, 737, 1030
Hpy188I TCNGA 8 cut(s) 496, 511, 594, 628, 686, 977, 984, 997
Hpy188III TCNNGA 6 cut(s) 136, 331, 368, 701, 1023, 1031
Hpy8I GTNNAC 5 cut(s) 222, 282, 469, 737, 1030
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 3 cut(s) 34, 195, 648
HpyCH4III ACNGT 4 cut(s) 375, 680, 924, 935
HpyCH4V TGCA 6 cut(s) 142, 196, 222, 352, 431, 947
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 644
HpyF3I CTNAG 2 cut(s) 156, 690
Hsp92II CATG 6 cut(s) 256, 316, 356, 403, 529, 955
HspAI GCGC 1 cut(s) 1007
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 3 cut(s) 706, 784, 972
LmnI GCTCC 4 cut(s) 77, 445, 487, 964
LweI GCATC 1 cut(s) 129
MaeI CTAG 2 cut(s) 599, 726
MaeIII GTNAC 2 cut(s) 308, 337
MalI GATC 3 cut(s) 708, 786, 974
MbiI CCGCTC 1 cut(s) 72
MboI GATC 3 cut(s) 706, 784, 972
MboII GAAGA 3 cut(s) 584, 896, 1036
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 224, 276
MlsI TGGCCA 1 cut(s) 753
MluCI AATT 3 cut(s) 103, 207, 230
MluNI TGGCCA 1 cut(s) 753
MmeI TCCRAC 1 cut(s) 357
Mox20I TGGCCA 1 cut(s) 753
MscI TGGCCA 1 cut(s) 753
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 933
Msp20I TGGCCA 1 cut(s) 753
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 3 cut(s) 422, 518, 908
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 3 cut(s) 422, 518, 908
MwoI GCNNNNNNNGC 2 cut(s) 422, 644
NdeI CATATG 1 cut(s) 304
NdeII GATC 3 cut(s) 706, 784, 972
NlaIII CATG 6 cut(s) 256, 316, 356, 403, 529, 955
NlaIV GGNNCC 3 cut(s) 166, 346, 607
NmuCI GTSAC 1 cut(s) 308
NspI RCATGY 2 cut(s) 256, 403
NspV TTCGAA 1 cut(s) 12
PaeR7I CTCGAG 1 cut(s) 699
PflMI CCANNNNNTGG 2 cut(s) 394, 865
PkrI GCNGC 1 cut(s) 91
Ple19I CGATCG 1 cut(s) 709
Psp6I CCWGG 3 cut(s) 420, 516, 906
PspGI CCWGG 3 cut(s) 420, 516, 906
PspN4I GGNNCC 3 cut(s) 166, 346, 607
PspPI GGNCC 1 cut(s) 164
PsrI GAACNNNNNNTAC 4 cut(s) 539, 571, 679, 711
PstNI CAGNNNCTG 2 cut(s) 373, 865
PvuI CGATCG 1 cut(s) 709
RsaI GTAC 4 cut(s) 5, 44, 161, 722
RsaNI GTAC 4 cut(s) 4, 43, 160, 721
RseI CAYNNNNRTG 1 cut(s) 933
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 3 cut(s) 706, 784, 972
Sau96I GGNCC 1 cut(s) 164
ScrFI CCNGG 3 cut(s) 422, 518, 908
SduI GDGCHC 2 cut(s) 224, 276
SfaNI GCATC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 699
SfuI TTCGAA 1 cut(s) 12
SlaI CTCGAG 1 cut(s) 699
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 1 cut(s) 699
SmoI CTYRAG 1 cut(s) 699
Sse9I AATT 3 cut(s) 103, 207, 230
SsiI CCGC 7 cut(s) 39, 61, 70, 87, 90, 425, 442
SspI AATATT 1 cut(s) 667
SspMI CTAG 2 cut(s) 599, 726
StyD4I CCNGG 3 cut(s) 420, 516, 906
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 4 cut(s) 375, 680, 924, 935
TaqI TCGA 4 cut(s) 12, 126, 700, 827
TasI AATT 3 cut(s) 103, 207, 230
TatI WGTACW 1 cut(s) 720
TauI GCSGC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 119, 160
TseFI GTSAC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 3 cut(s) 542, 897, 1036
TspRI CASTG 2 cut(s) 119, 160
Van91I CCANNNNNTGG 2 cut(s) 394, 865
VneI GTGCAC 1 cut(s) 220
XceI RCATGY 2 cut(s) 256, 403
XhoI CTCGAG 1 cut(s) 699
XspI CTAG 2 cut(s) 599, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.