Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0005867

Physical Location & Seq

Genomic Coordinates Forward (+)

4831075 .. 4834458

Gene Structure 3384 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom17g06660.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3384 bp

ATGGCATTTCACTTATCATCCGAAGAGGAAAAAACATCACAGGCGAAATTAATATTTTCTGCTCTTAGAGCAGTTGGTGCTCCAACTTCGGGTTTTAACAGTACACATTTGTCTTGCTGGGCTCTACTGGTTTCAAGGTTGATTTTAGTGCTTCGCCATATGATATTTTACCCACAGACTTGTCCTTCGTCCTTGCTTGTACCTCTGCGGTCCAAGTTGAGGGAGGCTCCGTACTCCAGTTCACAGCTTGGAGCGAACGATCATTTGTCATCTTGGGTCTCAGTGGTATTTAAGAATGTAATGAGCGCATCATTTGAAGAAGAGTCTGACATCAGTCCCTTGATTCATCAACTGATTGATACATCTGCCTTCCCTGCTTCACAGTCTCTAGATCATGTGGATGTTGACTCCTTATGCTTGAACTGGTACGAGATCTGTTCAACCATTTCGTCAATTCTGGGATTGTGGAAAGGCAAACAGGCTGCAGCGGTGGAGGATCTTATTGTTGAAAGATACATTTTCATACTTTGTTGGGATTTTCCAACTCTAGGTACTTCAAGGGAACGTCAGCTTCCTTCAAGTAGCAATCCACAGACTTTTGACGTTTCTGACATGGAAAACTTCTTTTTCTTCAGCCATTCAATTCTAGGTCATCAGGCTGCTGTTGATGCGAAAACTAATTCTCAAGTTGTAGTTCAGCTGCTTCAGCACTTATCTGAGCACACTGAGGAAGTAGGCTGGGGTTTCTTGAGAAATGCAAGATGGCTGTCTTTGGCACTCACCTTGCTTGATGTTGGGATCTCGAGGTATGGCATGAAGAATAAAGTCCCAGGAGTGGGTTCAGACTGGATAGAGAACATGTCCAAGGACAATGAGTATATTGCTGTTGCTGAAGGTCTGATTGCCTCTTTAATGAACGCTGATCAAGTTTCGATTTTGTTGAAGACATTTTCATCTTTGCTGAACAGTTATTTACTGGCTTATCAGAAAGCTTTTGTGGCTACCTTTGGTAATAGCCAGAAGGATGCTGATGGGTTTTCACCATTGTTGCTTTTTAAGCATTCAGGATTTGACAAATGCCTTCAGGATGAGCTAGGAAAGACTGGAACTTATTCGTTCCATCTGGAGTCTGTTATTGACCCCTTGGCTAAGTTTGACGCTATTATTGACAAAAGAGCTTCAGGAATTTTGTGTGGGGCCTCCTGGCAATGTATGCTGCATGGTTTCCCACTGAATCTTCAGACTTCCAGTGGTGTTCTCCTTTCTTGCATTCTTAGCATTAGAGGGATCATTTCCATTCTGGTTGGTCTGCTTAAAATGAAAGATATGATAGGAAATGTCTGCTTGGAGAATGAAGTGCTTCATCAAATTCTTGATATGGCAGTGACAATCAAGTCTGATAGGATTTTTGAAAGCATTCATGGGAAATGTGAAACCATATATGATAGCTTGAGTGTAGGTTTGGGAGGATCAGACTGTGCCAATTTAATTCTATTAGAGCACCTAGAAGGATTTTTAAGGGATATCAATGCCAGAGGAGTAAGTGACAGCACTATCCATGAGTGCATAATTACCAAAGCTATTGATACAATGGATAGCCTAAGGAAAGACCCTGCAACGGTTGATTATTTTAAATTCTATCTTGGTAAAGAAGATGTCCCCGAGCAGGTGAAGGAGCTATTTGGTGTGCAGCGTGGCAATCTACTGGTTCTAATTGACTCTTTACATAATTGTTACTCTGAAACAGTTAACATAAGGGTTCTTAACTTTTTTGTTGATCTGTTAACTGGAGAGCAGTTTCCTGATCTTAAACAGAAGATCCAAAACAAGTTTCTTGGCATGGACTTGATTTTGCTTTCCAAATGGTTGGAGAAGAGGCTGTTAGGTTGTGTAACAGAAGCTTCAGGTGGAGTTAATAGTTCAAAAGGAAGTTCCATCTCTCTGAGAGAGTCAACTATGAACTTTATTTTATGCATTGTATCGTCACCTCCTAATTTGAATTCAACAGAACTGCAGAGTCATATTTTTGAAGCTGTTCTGGTTTCCCTTGGACCTGCATTTTTGCAATTTGATATTCATGTTGCCAAATCATTTTTCCAATTTGTTGTTCAACTGTCTAAGGGGGATGCTTCAGTGAGATTGCTTTTAAAGAAGACCATAATGCTGATGGAAAAGTTATCTGGCAATGATTGCCTGCTACCAGGTCTGAAGTTTCTTTTTGGCTTTTTTGGGAGTGTCTTAAGTGATTGTGGGTCTGGCAAGAATACCTTAGAAAGGTCGTCCGGGAAGTCTCTGCCTGTAAATGCTCTTGGTGGTGTGGGACCTATGGCTTCAAGGCCAGTAGGATCGAGGAAAAACTCCGAGGCATTGGTCCTTTCTTCTAATCAAGAAGATGGTTCCATCGCTCTTGAATGTGATGCAACTTCTGTAGACGAGGATGAGGATGATGGAACTTCTGATGGTGAGGTGGCCAGCCTGGACAAGGATGATGAAGAGGATACTAACAGTGAAAGGGCCCTTGCTTCTAAAGTTTGCACATTTACTTCTAGTGGTAGCAATTTCATGGAACAACATTGGTACTTCTGTTATACATGTGATCTGACAGTGTCAAAAGGCTGTTGCTCTGTGTGTGCAAAAGTTTGTCATCGCGGTCATCGTGTTGTTTATTCTCGCTCTAGTCGTTTCTTTTGTGACTGTGGAGCTGGGGGTGTTAGAGGAAGCAACTGTCAGTGCTTAAAGCCACGCAAGTTCACTGGAAGTAGTAGCGCTCCCGTCCATAGTGCTAGTAATTTCCAGGCTCTATTACCCTTCACGGAGGATGGAGAGCAGCTTCCTGAAAGTGATTCAGATTTTGATGAGGATACTAGTACTGAGGTAGATAACTCATCCATCCCAAGAGAGCTTCAAGATGGGATCGAACCATTGCTTGAAGAACTTGATGTTGAAGGTCAGGTGCTTGAACTTTGCTCTTCATTGTTCCCATACATAACTTGTAAAAGAGACTCGAACTTGTCAAAAGATAATAAAATCGCTCTTGGTAAAGACAAAGTGCTGTCTTTTGGTGTTGATCTCTTGCAATTGAAGAAGGCATATAAAAGTGGGTCCTTAGACCTGAAGGTAAAGGCCGACTATTCAAATGCCAAAGAACTTAAATCACATTTAGCTAGTGGGTCTCTTGTGAAATCCTTGCTCAGTGTCAGTATTCGAGGGCGACTTGCTGTTGGAGAAGGTGATAAAGTTGCTATATTTGATGTTGTGCAGTTGATTGGACAGGCCACCATTGCACCAGTGACGGCAGACAAGACCAATGTTAAGCCTCTCTCTAAGAATGTTGTTCGGTTTGAAATCGTGCATCTTACTTTCAATCCAGTGGTAGAGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006810 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009826 GO:0009914 GO:0009926 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010101 GO:0010102 GO:0010229 GO:0010311 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0022414 GO:0022622 GO:0030054 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042221 GO:0043207 GO:0044424 GO:0044444 GO:0044464 GO:0048281 GO:0048364 GO:0048367 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055044 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko05165 ko05203 map05165 map05203

Pfam Domains

Protein Families

E3_UbLigase_R4 ZZ

Protein Analysis

1128

Amino Acids

123.47

Weight (kDa)

5.28

Isoelectric Point (pI)

40.21

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005867)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02260 AT3G02260 AT3G02260 AT3G02260
fragaria_vesca	FvH4_6g46320 FvH4_6g46320
malus_domestica	MD09G1075600.v1.1 MD17G1066300.v1.1
prunus_persica	Prupe.3G248400_v2.0.a1 Prupe.3G248400_v2.0.a1 Prupe.3G248400_v2.0.a1
pyrus_communis	pycom09g00280 pycom09g00290 pycom09g00300 pycom17g06650 pycom17g06660 pycom17g06670 pycom17g06680
rosa_chinensis	RchiOBHm_Chr2g0164791
rosa_laevigata	RLG00000021489
rosa_multiflora	Rmu_sc0000946.1_g000029
rosa_roxburghii	Rroxscaffold_2G00086300
rosa_rugosa	Rorug02G0510400
rosa_samantha	Rh2AG578500 Rh2BG589800 Rh2CG560300 Rh2DG600000
rosa_wichuraiana	Rw2G048030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1657
AasI	GACNNNNNNGTC	1 cut(s)	1393
Acc36I	ACCTGC	2 cut(s)	1657, 2062
AccI	GTMKAC	1 cut(s)	2430
AccII	CGCG	1 cut(s)	2649
AciI	CCGC	3 cut(s)	208, 488, 2649
AclWI	GGATC	7 cut(s)	504, 806, 1295, 1477, 1813, 2353, 2921
AcoI	YGGCCR	1 cut(s)	2469
AcsI	RAATTY	4 cut(s)	1185, 1368, 1634, 1999
AfaI	GTAC	7 cut(s)	103, 201, 233, 428, 553, 2579, 2869
AfeI	AGCGCT	1 cut(s)	2767
AfiI	CCNNNNNNNGG	9 cut(s)	89, 219, 548, 835, 836, 1618, 1666, 2274, 2482
AflII	CTTAAG	1 cut(s)	2239
AflIII	ACRYGT	2 cut(s)	858, 2591
AhlI	ACTAGT	1 cut(s)	2864
AjnI	CCWGG	5 cut(s)	829, 1202, 2200, 2475, 2793
AjuI	GAANNNNNNNTTGG	6 cut(s)	1815, 1847, 2078, 2091, 2110, 2123
Alw21I	GWGCWC	3 cut(s)	82, 723, 1503
Alw26I	GTCTC	5 cut(s)	283, 390, 2295, 2994, 3177
AlwI	GGATC	7 cut(s)	504, 806, 1295, 1477, 1813, 2353, 2921
Ama87I	CYCGRG	2 cut(s)	802, 1661
Aor51HI	AGCGCT	1 cut(s)	2767
AoxI	GGCC	6 cut(s)	1197, 2336, 2469, 2514, 3123, 3273
ApaI	GGGCCC	1 cut(s)	2518
ApeKI	GCWGC	7 cut(s)	482, 485, 659, 700, 1216, 1690, 2827
ApoI	RAATTY	4 cut(s)	1185, 1368, 1634, 1999
ArsI	GACNNNNNNTTYG	2 cut(s)	169, 201
AseI	ATTAAT	1 cut(s)	50
Asp700I	GAANNNNTTC	4 cut(s)	1111, 1359, 1416, 2034
AspLEI	GCGC	2 cut(s)	308, 2768
AspS9I	GGNCC	8 cut(s)	210, 1197, 2051, 2321, 2371, 2514, 2515, 3102
AsuC2I	CCSGG	1 cut(s)	2284
AsuHPI	GGTGA	6 cut(s)	772, 1032, 1681, 1977, 2474, 3242
AvaI	CYCGRG	2 cut(s)	802, 1661
AvaII	GGWCC	5 cut(s)	210, 2051, 2321, 2371, 3102
AxyI	CCTNAGG	1 cut(s)	1601
BaeGI	GKGCMC	1 cut(s)	2518
BaeI	ACNNNNGTAYC	2 cut(s)	2569, 2602
BalI	TGGCCA	1 cut(s)	2471
BanII	GRGCYC	2 cut(s)	124, 2518
BbsI	GAAGAC	2 cut(s)	950, 2159
Bbv12I	GWGCWC	3 cut(s)	82, 723, 1503
BbvI	GCAGC	7 cut(s)	469, 497, 646, 687, 1203, 1702, 2839
BceAI	ACGGC	1 cut(s)	3309
BciT130I	CCWGG	5 cut(s)	831, 1204, 2202, 2477, 2795
BciVI	GTATCC	2 cut(s)	2491, 2854
BclI	TGATCA	1 cut(s)	922
BcnI	CCSGG	1 cut(s)	2284
BcoDI	GTCTC	5 cut(s)	283, 390, 2295, 2994, 3177
BcuI	ACTAGT	1 cut(s)	2864
BfmI	CTRYAG	3 cut(s)	483, 2012, 2427
BfoI	RGCGCY	1 cut(s)	2769
BfrI	CTTAAG	1 cut(s)	2239
BfuAI	ACCTGC	2 cut(s)	1657, 2062
BfuI	GTATCC	2 cut(s)	2491, 2854
BglII	AGATCT	1 cut(s)	432
BisI	GCNGC	7 cut(s)	483, 486, 660, 701, 1217, 1691, 2828
BlsI	GCNGC	7 cut(s)	484, 487, 661, 702, 1218, 1692, 2829
BmcAI	AGTACT	1 cut(s)	2869
Bme1390I	CCNGG	6 cut(s)	831, 1204, 2202, 2284, 2477, 2795
Bme18I	GGWCC	5 cut(s)	210, 2051, 2321, 2371, 3102
BmeT110I	CYCGRG	2 cut(s)	802, 1661
BmgT120I	GGNCC	8 cut(s)	210, 1197, 2051, 2321, 2371, 2514, 2515, 3102
BmiI	GGNNCC	6 cut(s)	228, 1198, 2322, 2398, 2516, 3103
BmrFI	CCNGG	6 cut(s)	831, 1204, 2202, 2284, 2477, 2795
BmsI	GCATC	6 cut(s)	317, 658, 1015, 2116, 2407, 3361
BoxI	GACNNNNGTC	1 cut(s)	333
BpiI	GAAGAC	2 cut(s)	950, 2159
BplI	GAGNNNNNCTC	2 cut(s)	211, 243
BpmI	CTGGAG	3 cut(s)	220, 1145, 1809
BpuEI	CTTGAG	3 cut(s)	669, 769, 1471
BpuMI	CCSGG	1 cut(s)	2284
BsaI	GGTCTC	2 cut(s)	283, 3177
BsaJI	CCNNGG	5 cut(s)	829, 864, 1143, 2047, 2361
BsaXI	ACNNNNNCTCC	2 cut(s)	215, 245
Bsc4I	CCNNNNNNNGG	9 cut(s)	89, 219, 548, 835, 836, 1618, 1666, 2274, 2482
Bse21I	CCTNAGG	1 cut(s)	1601
Bse3DI	GCAATG	4 cut(s)	1214, 2191, 2921, 3279
BseBI	CCWGG	5 cut(s)	831, 1204, 2202, 2477, 2795
BseDI	CCNNGG	5 cut(s)	829, 864, 1143, 2047, 2361
BseLI	CCNNNNNNNGG	9 cut(s)	89, 219, 548, 835, 836, 1618, 1666, 2274, 2482
BseMI	GCAATG	4 cut(s)	1214, 2191, 2921, 3279
BseMII	CTCAG	6 cut(s)	294, 708, 717, 1934, 2862, 3205
BseRI	GAGGAG	1 cut(s)	1551
BseSI	GKGCMC	1 cut(s)	2518
BseXI	GCAGC	7 cut(s)	469, 497, 646, 687, 1203, 1702, 2839
BseYI	CCCAGC	3 cut(s)	117, 738, 2702
BsgI	GTGCAG	2 cut(s)	1709, 3278
Bsh1236I	CGCG	1 cut(s)	2649
BshFI	GGCC	6 cut(s)	1199, 2338, 2471, 2516, 3125, 3275
BsiHKAI	GWGCWC	3 cut(s)	82, 723, 1503
BsiHKCI	CYCGRG	2 cut(s)	802, 1661
BsiSI	CCGG	1 cut(s)	2283
BslFI	GGGAC	4 cut(s)	321, 812, 1643, 2334
BslI	CCNNNNNNNGG	9 cut(s)	89, 219, 548, 835, 836, 1618, 1666, 2274, 2482
BsmAI	GTCTC	5 cut(s)	283, 390, 2295, 2994, 3177
BsmFI	GGGAC	4 cut(s)	321, 812, 1643, 2334
BsmI	GAATGC	3 cut(s)	1060, 1269, 1416
BsnI	GGCC	6 cut(s)	1199, 2338, 2471, 2516, 3125, 3275
Bso31I	GGTCTC	2 cut(s)	283, 3177
BsoBI	CYCGRG	2 cut(s)	802, 1661
Bsp120I	GGGCCC	1 cut(s)	2514
Bsp1286I	GDGCHC	5 cut(s)	82, 124, 723, 1503, 2518
BspACI	CCGC	3 cut(s)	208, 488, 2649
BspANI	GGCC	6 cut(s)	1199, 2338, 2471, 2516, 3125, 3275
BspCNI	CTCAG	6 cut(s)	293, 709, 718, 1935, 2863, 3204
BspFNI	CGCG	1 cut(s)	2649
BspLI	GGNNCC	6 cut(s)	228, 1198, 2322, 2398, 2516, 3103
BspMAI	CTGCAG	2 cut(s)	487, 2016
BspMI	ACCTGC	2 cut(s)	1657, 2062
BspPI	GGATC	7 cut(s)	504, 806, 1295, 1477, 1813, 2353, 2921
BspQI	GCTCTTC	1 cut(s)	2974
BspTI	CTTAAG	1 cut(s)	2239
BspTNI	GGTCTC	2 cut(s)	283, 3177
BsrDI	GCAATG	4 cut(s)	1214, 2191, 2921, 3279
BssECI	CCNNGG	5 cut(s)	829, 864, 1143, 2047, 2361
BssT1I	CCWWGG	3 cut(s)	864, 1143, 2047
Bst2UI	CCWGG	5 cut(s)	831, 1204, 2202, 2477, 2795
Bst6I	CTCTTC	5 cut(s)	18, 315, 1868, 2487, 2974
BstAFI	CTTAAG	1 cut(s)	2239
BstAPI	GCANNNNNTGC	3 cut(s)	77, 1213, 2190
BstC8I	GCNNGC	2 cut(s)	2195, 2473
BstENI	CCTNNNNNAGG	2 cut(s)	2272, 2480
BstFNI	CGCG	1 cut(s)	2649
BstH2I	RGCGCY	1 cut(s)	2769
BstHHI	GCGC	2 cut(s)	308, 2768
BstMAI	GTCTC	5 cut(s)	283, 390, 2295, 2994, 3177
BstNI	CCWGG	5 cut(s)	831, 1204, 2202, 2477, 2795
BstNSI	RCATGY	2 cut(s)	862, 2595
BstPAI	GACNNNNGTC	1 cut(s)	333
BstSCI	CCNGG	6 cut(s)	829, 1202, 2200, 2282, 2475, 2793
BstSFI	CTRYAG	3 cut(s)	483, 2012, 2427
BstSLI	GKGCMC	1 cut(s)	2518
BstUI	CGCG	1 cut(s)	2649
BstV1I	GCAGC	7 cut(s)	469, 497, 646, 687, 1203, 1702, 2839
BstV2I	GAAGAC	2 cut(s)	950, 2159
BstX2I	RGATCY	4 cut(s)	432, 496, 798, 1818
BstXI	CCANNNNNNTGG	1 cut(s)	1867
BstYI	RGATCY	4 cut(s)	432, 496, 798, 1818
Bsu36I	CCTNAGG	1 cut(s)	1601
BsuI	GTATCC	2 cut(s)	2491, 2854
BsuRI	GGCC	6 cut(s)	1199, 2338, 2471, 2516, 3125, 3275
BtgZI	GCGATG	2 cut(s)	2386, 2630
BtsI	GCAGTG	1 cut(s)	1389
BveI	ACCTGC	2 cut(s)	1657, 2062
Cac8I	GCNNGC	2 cut(s)	2195, 2473
CfoI	GCGC	2 cut(s)	308, 2768
Cfr13I	GGNCC	8 cut(s)	210, 1197, 2051, 2321, 2371, 2514, 2515, 3102
CseI	GACGC	1 cut(s)	1166
CsiI	ACCWGGT	1 cut(s)	2200
Csp6I	GTAC	7 cut(s)	102, 200, 232, 427, 552, 2578, 2868
CviQI	GTAC	7 cut(s)	102, 200, 232, 427, 552, 2578, 2868
DraI	TTTAAA	2 cut(s)	1633, 2148
DrdI	GACNNNNNNGTC	1 cut(s)	1393
DseDI	GACNNNNNNGTC	1 cut(s)	1393
EaeI	YGGCCR	1 cut(s)	2469
Eam1104I	CTCTTC	5 cut(s)	18, 315, 1868, 2487, 2974
EarI	CTCTTC	5 cut(s)	18, 315, 1868, 2487, 2974
Eco130I	CCWWGG	3 cut(s)	864, 1143, 2047
Eco24I	GRGCYC	2 cut(s)	124, 2518
Eco31I	GGTCTC	2 cut(s)	283, 3177
Eco32I	GATATC	1 cut(s)	1525
Eco47I	GGWCC	5 cut(s)	210, 2051, 2321, 2371, 3102
Eco47III	AGCGCT	1 cut(s)	2767
Eco81I	CCTNAGG	1 cut(s)	1601
Eco88I	CYCGRG	2 cut(s)	802, 1661
EcoNI	CCTNNNNNAGG	2 cut(s)	2272, 2480
EcoO109I	RGGNCCY	5 cut(s)	1197, 2321, 2514, 2515, 3102
EcoRI	GAATTC	1 cut(s)	1999
EcoRII	CCWGG	5 cut(s)	829, 1202, 2200, 2475, 2793
EcoRV	GATATC	1 cut(s)	1525
EcoT14I	CCWWGG	3 cut(s)	864, 1143, 2047
EcoT22I	ATGCAT	1 cut(s)	1976
EcoT38I	GRGCYC	2 cut(s)	124, 2518
ErhI	CCWWGG	3 cut(s)	864, 1143, 2047
FalI	AAGNNNNNCTT	2 cut(s)	2252, 2284
FaqI	GGGAC	4 cut(s)	321, 812, 1643, 2334
FauNDI	CATATG	1 cut(s)	159
FbaI	TGATCA	1 cut(s)	922
FblI	GTMKAC	1 cut(s)	2430
Fnu4HI	GCNGC	7 cut(s)	483, 486, 660, 701, 1217, 1691, 2828
FriOI	GRGCYC	2 cut(s)	124, 2518
Fsp4HI	GCNGC	7 cut(s)	483, 486, 660, 701, 1217, 1691, 2828
GlaI	GCGC	2 cut(s)	307, 2767
GluI	GCNGC	7 cut(s)	483, 486, 660, 701, 1217, 1691, 2828
GsaI	CCCAGC	3 cut(s)	121, 742, 2706
GsuI	CTGGAG	3 cut(s)	220, 1145, 1809
HaeII	RGCGCY	1 cut(s)	2769
HaeIII	GGCC	6 cut(s)	1199, 2338, 2471, 2516, 3125, 3275
HapII	CCGG	1 cut(s)	2283
HgaI	GACGC	1 cut(s)	1166
HhaI	GCGC	2 cut(s)	308, 2768
Hin6I	GCGC	2 cut(s)	306, 2766
HinP1I	GCGC	2 cut(s)	306, 2766
HincII	GTYRAC	4 cut(s)	406, 1750, 1785, 1953
HindII	GTYRAC	4 cut(s)	406, 1750, 1785, 1953
HindIII	AAGCTT	2 cut(s)	990, 1899
HpaI	GTTAAC	2 cut(s)	1750, 1785
HpaII	CCGG	1 cut(s)	2283
HphI	GGTGA	6 cut(s)	772, 1032, 1681, 1977, 2474, 3242
Hpy166II	GTNNAC	8 cut(s)	104, 242, 406, 1750, 1785, 1953, 2431, 2751
Hpy8I	GTNNAC	8 cut(s)	104, 242, 406, 1750, 1785, 1953, 2431, 2751
HpyCH4IV	ACGT	2 cut(s)	565, 603
HpySE526I	ACGT	2 cut(s)	565, 603
HspAI	GCGC	2 cut(s)	306, 2766
Ksp22I	TGATCA	1 cut(s)	922
KspAI	GTTAAC	2 cut(s)	1750, 1785
LguI	GCTCTTC	1 cut(s)	2974
LmnI	GCTCC	6 cut(s)	85, 232, 251, 1675, 2699, 2773
Lsp1109I	GCAGC	7 cut(s)	469, 497, 646, 687, 1203, 1702, 2839
LweI	GCATC	6 cut(s)	317, 658, 1015, 2116, 2407, 3361
MabI	ACCWGGT	1 cut(s)	2200
MaeII	ACGT	2 cut(s)	565, 603
MaeIII	GTNAC	7 cut(s)	1384, 1544, 1733, 1891, 1983, 2690, 3289
MfeI	CAATTG	1 cut(s)	3077
MflI	RGATCY	4 cut(s)	432, 496, 798, 1818
MhlI	GDGCHC	5 cut(s)	82, 124, 723, 1503, 2518
MlsI	TGGCCA	1 cut(s)	2471
MluNI	TGGCCA	1 cut(s)	2471
MlyI	GAGTC	7 cut(s)	332, 401, 1136, 1712, 1958, 2026, 2996
MmeI	TCCRAC	4 cut(s)	107, 566, 1848, 3202
Mox20I	TGGCCA	1 cut(s)	2471
Mph1103I	ATGCAT	1 cut(s)	1976
MroXI	GAANNNNTTC	4 cut(s)	1111, 1359, 1416, 2034
MscI	TGGCCA	1 cut(s)	2471
MslI	CAYNNNNRTG	1 cut(s)	399
Msp20I	TGGCCA	1 cut(s)	2471
MspA1I	CMGCKG	2 cut(s)	488, 700
MspCI	CTTAAG	1 cut(s)	2239
MspI	CCGG	1 cut(s)	2283
MspR9I	CCNGG	6 cut(s)	831, 1204, 2202, 2284, 2477, 2795
MunI	CAATTG	1 cut(s)	3077
Mva1269I	GAATGC	3 cut(s)	1060, 1269, 1416
MvaI	CCWGG	5 cut(s)	831, 1204, 2202, 2477, 2795
MvnI	CGCG	1 cut(s)	2649
NciI	CCSGG	1 cut(s)	2284
NdeI	CATATG	1 cut(s)	159
NlaIV	GGNNCC	6 cut(s)	228, 1198, 2322, 2398, 2516, 3103
NmuCI	GTSAC	5 cut(s)	1384, 1544, 1983, 2690, 3289
NsiI	ATGCAT	1 cut(s)	1976
NspI	RCATGY	2 cut(s)	862, 2595
PaeR7I	CTCGAG	1 cut(s)	802
PaqCI	CACCTGC	1 cut(s)	1657
PciI	ACATGT	2 cut(s)	858, 2591
PciSI	GCTCTTC	1 cut(s)	2974
PcsI	WCGNNNNNNNCGW	2 cut(s)	2653, 2677
PctI	GAATGC	3 cut(s)	1060, 1269, 1416
PdmI	GAANNNNTTC	4 cut(s)	1111, 1359, 1416, 2034
PfeI	GAWTC	3 cut(s)	343, 1234, 2843
PflFI	GACNNNGTC	1 cut(s)	2605
PfoI	TCCNGGA	1 cut(s)	2282
PkrI	GCNGC	7 cut(s)	484, 487, 661, 702, 1218, 1692, 2829
PleI	GAGTC	7 cut(s)	331, 401, 1135, 1712, 1957, 2025, 2996
PpsI	GAGTC	7 cut(s)	331, 401, 1135, 1712, 1957, 2025, 2996
PpuMI	RGGWCCY	2 cut(s)	2321, 3102
PscI	ACATGT	2 cut(s)	858, 2591
PshAI	GACNNNNGTC	1 cut(s)	333
PshBI	ATTAAT	1 cut(s)	50
Psp5II	RGGWCCY	2 cut(s)	2321, 3102
Psp6I	CCWGG	5 cut(s)	829, 1202, 2200, 2475, 2793
PspFI	CCCAGC	3 cut(s)	117, 738, 2702
PspGI	CCWGG	5 cut(s)	829, 1202, 2200, 2475, 2793
PspN4I	GGNNCC	6 cut(s)	228, 1198, 2322, 2398, 2516, 3103
PspOMI	GGGCCC	1 cut(s)	2514
PspPI	GGNCC	8 cut(s)	210, 1197, 2051, 2321, 2371, 2514, 2515, 3102
PspPPI	RGGWCCY	2 cut(s)	2321, 3102
PstI	CTGCAG	2 cut(s)	487, 2016
PsuI	RGATCY	4 cut(s)	432, 496, 798, 1818
PsyI	GACNNNGTC	1 cut(s)	2605
PvuII	CAGCTG	1 cut(s)	700
RsaI	GTAC	7 cut(s)	103, 201, 233, 428, 553, 2579, 2869
RsaNI	GTAC	7 cut(s)	102, 200, 232, 427, 552, 2578, 2868
RseI	CAYNNNNRTG	1 cut(s)	399
SapI	GCTCTTC	1 cut(s)	2974
SatI	GCNGC	7 cut(s)	483, 486, 660, 701, 1217, 1691, 2828
Sau96I	GGNCC	8 cut(s)	210, 1197, 2051, 2321, 2371, 2514, 2515, 3102
ScaI	AGTACT	1 cut(s)	2869
SchI	GAGTC	7 cut(s)	332, 401, 1136, 1712, 1958, 2026, 2996
ScrFI	CCNGG	6 cut(s)	831, 1204, 2202, 2284, 2477, 2795
SduI	GDGCHC	5 cut(s)	82, 124, 723, 1503, 2518
SexAI	ACCWGGT	1 cut(s)	2200
SfaNI	GCATC	6 cut(s)	317, 658, 1015, 2116, 2407, 3361
SfcI	CTRYAG	3 cut(s)	483, 2012, 2427
Sfr274I	CTCGAG	1 cut(s)	802
SinI	GGWCC	5 cut(s)	210, 2051, 2321, 2371, 3102
SlaI	CTCGAG	1 cut(s)	802
SmiMI	CAYNNNNRTG	1 cut(s)	399
SmlI	CTYRAG	5 cut(s)	684, 748, 802, 1450, 2239
SmoI	CTYRAG	5 cut(s)	684, 748, 802, 1450, 2239
SpeI	ACTAGT	1 cut(s)	2864
SsiI	CCGC	3 cut(s)	208, 488, 2649
SspI	AATATT	1 cut(s)	54
StyD4I	CCNGG	6 cut(s)	829, 1202, 2200, 2282, 2475, 2793
StyI	CCWWGG	3 cut(s)	864, 1143, 2047
TaiI	ACGT	2 cut(s)	568, 606
TaqI	TCGA	6 cut(s)	803, 932, 2348, 2916, 3005, 3205
TatI	WGTACW	2 cut(s)	101, 2867
TfiI	GAWTC	3 cut(s)	343, 1234, 2843
TseFI	GTSAC	5 cut(s)	1384, 1544, 1983, 2690, 3289
TseI	GCWGC	7 cut(s)	482, 485, 659, 700, 1216, 1690, 2827
Tsp45I	GTSAC	5 cut(s)	1384, 1544, 1983, 2690, 3289
TspGWI	ACGGA	2 cut(s)	219, 2828
Tth111I	GACNNNGTC	1 cut(s)	2605
Vha464I	CTTAAG	1 cut(s)	2239
VpaK11BI	GGWCC	5 cut(s)	210, 2051, 2321, 2371, 3102
VspI	ATTAAT	1 cut(s)	50
XagI	CCTNNNNNAGG	2 cut(s)	2272, 2480
XapI	RAATTY	4 cut(s)	1185, 1368, 1634, 1999
XbaI	TCTAGA	1 cut(s)	388
XceI	RCATGY	2 cut(s)	862, 2595
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2164
XhoI	CTCGAG	1 cut(s)	802
XmiI	GTMKAC	1 cut(s)	2430
XmnI	GAANNNNTTC	4 cut(s)	1111, 1359, 1416, 2034
ZrmI	AGTACT	1 cut(s)	2869
Zsp2I	ATGCAT	1 cut(s)	1976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.