Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0005867

Physical Location & Seq

Genomic Coordinates Forward (+)

4843937 .. 4846709

Gene Structure 2773 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGCATTGCTGTAAAATAAGAGAGAACAGACGAGCTTTGCTGAGGTTAGGAGCTTTAGGTCTACTCCTTGAAACGGCAAGACATGCCTTTTCTGTTGATGCCATGGAGCCAGCTGAAGGCATTCTTCTCATTGTTGAGAGTCTGACTCTGGAAGCTAATGAAAGTGATAACATTAGCATCACACAAAGTGCTCTTACTGTCACTAGTGAAGAGACCGGTGAACAGGCCAAGAAAATAGTCCTCATGTTTCTGGAGAGATTATCTCATCCTTTGGGCCTTAAGAAATCAAACAAACAGCAGAGGAACAATGAGATGGTTGCAAGAATCTTGCCCTACTTGACATATGGTGAACCTGCTGCAATGGAAGCGCTTATCCAACACTTCAGCCCACCCTTGCAGGACTGGAGGGAATACGATCGGCTGCAAAGAGAACATGAAGCTGACCCAAAAGATGAAAGCATCGCCCAGCAAGCTGCCAAGCAGAGGTTCACGTTGGAAAATTTTGTAAGGGTATCTGAGTCTCTTAAAACAAGTTCCTGCGGAGATAGACTGAAAGACATTATTCTTGAGAGGGGCATTACTGGCGTTGCAGTTAGACATCTGAGGGATAGTTTTTCAGCTGCGGGACAGGCTGGCTTTAAATCTAGTGCAGAATGGGCAATGGGCTTGACACTGCCATCTGTCCCACTGATATTGTCTATGCTGAGAGGCTTGTCGACAGGACATTTGGCCACTCAAAAGTGTATTGATGAGGGAGGGATTTTACCTTTACTTCATGCTTTGGAAGGGGTCCCGGGGGAAAACGAGATAGGCGCGAGGGCTGAGAACTTATTGGACACCCTTTCAAATAAGGAGGGAAAAGGAGATGGCTTTTTGGAGGAGAAGGTGCGGAGGTTGAGACATGCCACCAGGGATGAAATGAGGCGTAGAGCTCTAAGGAGAAGAGAGGAATTGCTTCTGGGTCTTGGAATGCGACAAGAGCTAGCTTCACATGGCGGTGAGCGTATTGTTGTTTCACGACCATTACTTGAAGGTTTAGAAGACGTGGAGGAAGAGGAGGAGGATGGCTTAGCATGCATGGTTTGCCGAGAGGGTTACAGTCTGAGGCCTACTGACCTACTTGGTGTTTACTCTTATAGCAAGCGAGTGAATTTAGGTGCAGGGACTTCTGGAAGCGCACGTGGGGAGTGTGTTTATACAACTGTTAGTTATTTCAACATTATTCATTTCCAGTGCCATCAGGAAGCCAAGAGAGCAGATGCTGCTCTGAAAAATCCAAAGAAGGAGTGGGAAGGAGCAACTCTCAGAAATAATGAATCTCTTTGCAATTCTTTGTTCCCTGTGAGAGGTCCGTCTGTTCCTTTAGCGCAGTATATTCGCTATGTTGACCAGTACTGGGACAACCTCAATGCTCTTGGACGTGCTGATGGAAGCCGCCTTCGGCTGTTGACTTATGACATTGTTCTGATGCTAGCTCGTTTTGCGACCGGGGCATCATTTAGTGCAGAATGCAGAGGTGGTGGAAGGGAAAGCAACTCCAGATTTCTTCCTTTCATGATCCAAATGGCACGTCACCTTCTCGATCAGGGAAGCTCATCTCAGCGTCATACAATGGCCAAGTCTCTGACAGCATATTTGTCATCGTCTGCATCAGATTCTAGGACTTCTACTCCTGAGAAACAACCGTCTATGGGATCTGAAGAAACTGTCCAGTTTATGATGGTGAATTCACTTCTTTCAGAATCTCATGAATCCTGGTTGCAACACCGCCGTGCCTTCTTGCAGCGTGGAATATACCATGCCTACATGCAGCACACTCATGGTCGGTCAGCAGGTCGCACATCATCCAGTTCATCTCCTGTAGTCAATATAGAGTCTGGTAATACAAGCCAAAGTCCTAGTGCAGAGATTGGGGGTGCCGATGAGCTTTTGTCCGTTATTCGACCAATGCTTGTCTACACTGGCTTGATTGAGCAGTTGCAACGTTTCTTCAAGGTCAAAAAACCAGCAAATGCGGCACCGGCCCCAACAGAAGGTACATCGGGAGTATCTGAAGGGGATGACAGTGGAAGCTTGGAAGTTTGGGAGGTAGTGATGAAAGAGCGGCTTTTGAACGTAAAGGAGATGGTGGATTTCTCGAAGGAAATGCTATCATGGCTTGACGAGATGAGTTCCGCGAGCGATTTGCAGGAGGCATTTGACATAATCGGTGTTTTAGCGGATGTGCTGTCTGGTGGCATTACCAACTGTGAGGACTTTGTACGTGCAGCAATCGATGCGGGGCGAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006810 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009826 GO:0009914 GO:0009926 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010101 GO:0010102 GO:0010229 GO:0010311 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0022414 GO:0022622 GO:0030054 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042221 GO:0043207 GO:0044424 GO:0044444 GO:0044464 GO:0048281 GO:0048364 GO:0048367 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055044 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko05165 ko05203 map05165 map05203

Pfam Domains

Protein Families

E3_UbLigase_R4 ZZ

Protein Analysis

764

Amino Acids

84.4

Weight (kDa)

5.85

Isoelectric Point (pI)

50.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E3_UbLigase_R4	PF13764	1 - 737	2.4e-240	E3 ubiquitin-protein ligase UBR4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005867)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02260 AT3G02260 AT3G02260 AT3G02260
fragaria_vesca	FvH4_6g46320 FvH4_6g46320
malus_domestica	MD09G1075600.v1.1 MD17G1066300.v1.1
prunus_persica	Prupe.3G248400_v2.0.a1 Prupe.3G248400_v2.0.a1 Prupe.3G248400_v2.0.a1
pyrus_communis	pycom09g00280 pycom09g00290 pycom09g00300 pycom17g06650 pycom17g06660 pycom17g06670 pycom17g06680
rosa_chinensis	RchiOBHm_Chr2g0164791
rosa_laevigata	RLG00000021489
rosa_multiflora	Rmu_sc0000946.1_g000029
rosa_roxburghii	Rroxscaffold_2G00086300
rosa_rugosa	Rorug02G0510400
rosa_samantha	Rh2AG578500 Rh2BG589800 Rh2CG560300 Rh2DG600000
rosa_wichuraiana	Rw2G048030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	361, 1823
AccB1I	GGYRCC	2 cut(s)	1916, 2017
AccBSI	CCGCTC	1 cut(s)	2104
AccI	GTMKAC	3 cut(s)	61, 716, 1956
AccII	CGCG	2 cut(s)	815, 2177
AclI	AACGTT	1 cut(s)	1984
AclWI	GGATC	2 cut(s)	1552, 1702
AcoI	YGGCCR	2 cut(s)	729, 1614
AcsI	RAATTY	3 cut(s)	499, 1150, 1726
AcuI	CTGAAG	4 cut(s)	135, 367, 1719, 2073
AcvI	CACGTG	1 cut(s)	1181
AdeI	CACNNNGTG	1 cut(s)	188
AfaI	GTAC	3 cut(s)	1394, 2038, 2262
AfeI	AGCGCT	1 cut(s)	369
AfiI	CCNNNNNNNGG	7 cut(s)	73, 116, 483, 1346, 1396, 1691, 2033
AflII	CTTAAG	1 cut(s)	278
AgeI	ACCGGT	1 cut(s)	215
AgsI	TTSAA	6 cut(s)	71, 846, 1031, 1216, 1993, 2113
AhlI	ACTAGT	1 cut(s)	203
AjiI	CACGTC	3 cut(s)	1045, 1421, 1571
AjnI	CCWGG	2 cut(s)	908, 1754
AjuI	GAANNNNNNNTTGG	2 cut(s)	470, 502
AleI	CACNNNNGTG	1 cut(s)	1770
Alw21I	GWGCWC	2 cut(s)	193, 934
Alw26I	GTCTC	4 cut(s)	206, 525, 892, 1626
AlwI	GGATC	2 cut(s)	1552, 1702
AlwNI	CAGNNNCTG	1 cut(s)	1262
Ama87I	CYCGRG	1 cut(s)	793
Aor51HI	AGCGCT	1 cut(s)	369
AoxI	GGCC	6 cut(s)	225, 274, 729, 1106, 1614, 2022
ApeKI	GCWGC	8 cut(s)	356, 421, 473, 620, 1262, 1783, 1810, 2267
ApoI	RAATTY	3 cut(s)	499, 1150, 1726
ArsI	GACNNNNNNTTYG	6 cut(s)	709, 741, 1555, 1587, 1618, 1650
AsiGI	ACCGGT	1 cut(s)	215
Asp700I	GAANNNNTTC	2 cut(s)	120, 954
AspLEI	GCGC	4 cut(s)	370, 815, 1178, 1369
AspS9I	GGNCC	4 cut(s)	274, 790, 1349, 2023
AsuC2I	CCSGG	3 cut(s)	794, 795, 1489
AsuHPI	GGTGA	5 cut(s)	230, 359, 1010, 1565, 1735
AsuNHI	GCTAGC	2 cut(s)	982, 1471
AvaI	CYCGRG	1 cut(s)	793
AvaII	GGWCC	2 cut(s)	790, 1349
BalI	TGGCCA	2 cut(s)	731, 1616
BanI	GGYRCC	2 cut(s)	1916, 2017
BanII	GRGCYC	1 cut(s)	934
BbrPI	CACGTG	1 cut(s)	1181
BbsI	GAAGAC	1 cut(s)	1047
Bbv12I	GWGCWC	2 cut(s)	193, 934
BbvCI	CCTCAGC	1 cut(s)	41
BbvI	GCAGC	8 cut(s)	343, 408, 460, 607, 1249, 1795, 1822, 2279
BccI	CCATC	8 cut(s)	307, 685, 860, 1058, 1245, 1421, 1714, 2119
BceAI	ACGGC	2 cut(s)	90, 1755
BcgI	CGANNNNNNTGC	6 cut(s)	1358, 1392, 2128, 2162, 2167, 2201
BciT130I	CCWGG	2 cut(s)	910, 1756
BcnI	CCSGG	3 cut(s)	794, 795, 1489
BcoDI	GTCTC	4 cut(s)	206, 525, 892, 1626
BcuI	ACTAGT	1 cut(s)	203
BfaI	CTAG	6 cut(s)	204, 645, 983, 1472, 1659, 1899
BfmI	CTRYAG	1 cut(s)	1860
BfoI	RGCGCY	1 cut(s)	371
BfrI	CTTAAG	1 cut(s)	278
BfuAI	ACCTGC	2 cut(s)	361, 1823
BlpI	GCTNAGC	1 cut(s)	1069
BmcAI	AGTACT	1 cut(s)	1394
Bme1390I	CCNGG	5 cut(s)	794, 795, 910, 1489, 1756
Bme18I	GGWCC	2 cut(s)	790, 1349
BmeT110I	CYCGRG	1 cut(s)	793
BmgBI	CACGTC	3 cut(s)	1045, 1421, 1571
BmgT120I	GGNCC	4 cut(s)	274, 790, 1349, 2023
BmiI	GGNNCC	6 cut(s)	108, 791, 792, 1918, 2019, 2025
BmrFI	CCNGG	5 cut(s)	794, 795, 910, 1489, 1756
BmrI	ACTGGG	1 cut(s)	1405
BmsI	GCATC	8 cut(s)	88, 186, 468, 1249, 1458, 1502, 1658, 2266
BmtI	GCTAGC	2 cut(s)	986, 1475
BmuI	ACTGGG	1 cut(s)	1405
BpiI	GAAGAC	1 cut(s)	1047
BplI	GAGNNNNNCTC	6 cut(s)	130, 162, 247, 279, 1287, 1319
BpmI	CTGGAG	3 cut(s)	272, 424, 1522
Bpu10I	CCTNAGC	1 cut(s)	41
Bpu1102I	GCTNAGC	1 cut(s)	1069
BpuEI	CTTGAG	1 cut(s)	587
BpuMI	CCSGG	3 cut(s)	794, 795, 1489
Bsa29I	ATCGAT	1 cut(s)	2274
BsaAI	YACGTR	2 cut(s)	1181, 2264
BsaI	GGTCTC	1 cut(s)	206
BsaJI	CCNNGG	5 cut(s)	102, 793, 794, 909, 1488
BsaWI	WCCGGW	1 cut(s)	215
BsaXI	ACNNNNNCTCC	2 cut(s)	1654, 1684
Bsc4I	CCNNNNNNNGG	7 cut(s)	73, 116, 483, 1346, 1396, 1691, 2033
Bse118I	RCCGGY	2 cut(s)	215, 2020
Bse1I	ACTGG	8 cut(s)	407, 586, 1231, 1390, 1400, 1711, 1848, 1966
Bse3DI	GCAATG	3 cut(s)	4, 366, 666
BseBI	CCWGG	2 cut(s)	910, 1756
BseCI	ATCGAT	1 cut(s)	2274
BseDI	CCNNGG	5 cut(s)	102, 793, 794, 909, 1488
BseGI	GGATG	6 cut(s)	265, 919, 1069, 1844, 2065, 2227
BseLI	CCNNNNNNNGG	7 cut(s)	73, 116, 483, 1346, 1396, 1691, 2033
BseMI	GCAATG	3 cut(s)	4, 366, 666
BseMII	CTCAG	9 cut(s)	32, 507, 593, 695, 813, 1094, 1318, 1613, 1665
BseNI	ACTGG	8 cut(s)	407, 586, 1231, 1390, 1400, 1711, 1848, 1966
BseRI	GAGGAG	3 cut(s)	893, 1070, 1073
BseXI	GCAGC	8 cut(s)	343, 408, 460, 607, 1249, 1795, 1822, 2279
BseYI	CCCAGC	1 cut(s)	465
BsgI	GTGCAG	5 cut(s)	669, 1179, 1524, 1923, 2286
Bsh1236I	CGCG	2 cut(s)	815, 2177
Bsh1285I	CGRYCG	2 cut(s)	418, 1488
BshFI	GGCC	6 cut(s)	227, 276, 731, 1108, 1616, 2024
BshNI	GGYRCC	2 cut(s)	1916, 2017
BshTI	ACCGGT	1 cut(s)	215
BshVI	ATCGAT	1 cut(s)	2274
BsiEI	CGRYCG	2 cut(s)	418, 1488
BsiHKAI	GWGCWC	2 cut(s)	193, 934
BsiHKCI	CYCGRG	1 cut(s)	793
BsiSI	CCGG	4 cut(s)	216, 794, 1488, 2021
BslFI	GGGAC	5 cut(s)	639, 668, 776, 1177, 1412
BslI	CCNNNNNNNGG	7 cut(s)	73, 116, 483, 1346, 1396, 1691, 2033
BsmAI	GTCTC	4 cut(s)	206, 525, 892, 1626
BsmFI	GGGAC	5 cut(s)	639, 668, 776, 1177, 1412
BsmI	GAATGC	3 cut(s)	120, 975, 1514
BsnI	GGCC	6 cut(s)	227, 276, 731, 1108, 1616, 2024
Bso31I	GGTCTC	1 cut(s)	206
BsoBI	CYCGRG	1 cut(s)	793
Bsp1286I	GDGCHC	2 cut(s)	193, 934
Bsp143I	GATC	4 cut(s)	415, 1557, 1582, 1694
Bsp1720I	GCTNAGC	1 cut(s)	1069
Bsp19I	CCATGG	1 cut(s)	102
BspANI	GGCC	6 cut(s)	227, 276, 731, 1108, 1616, 2024
BspCNI	CTCAG	9 cut(s)	33, 508, 594, 696, 814, 1095, 1317, 1612, 1666
BspDI	ATCGAT	1 cut(s)	2274
BspFNI	CGCG	2 cut(s)	815, 2177
BspHI	TCATGA	2 cut(s)	1554, 1747
BspLI	GGNNCC	6 cut(s)	108, 791, 792, 1918, 2019, 2025
BspMI	ACCTGC	2 cut(s)	361, 1823
BspOI	GCTAGC	2 cut(s)	986, 1475
BspPI	GGATC	2 cut(s)	1552, 1702
BspT107I	GGYRCC	2 cut(s)	1916, 2017
BspTI	CTTAAG	1 cut(s)	278
BspTNI	GGTCTC	1 cut(s)	206
BsrBI	CCGCTC	1 cut(s)	2104
BsrDI	GCAATG	3 cut(s)	4, 366, 666
BsrFI	RCCGGY	2 cut(s)	215, 2020
BsrI	ACTGG	8 cut(s)	407, 586, 1231, 1390, 1400, 1711, 1848, 1966
BssAI	RCCGGY	2 cut(s)	215, 2020
BssECI	CCNNGG	5 cut(s)	102, 793, 794, 909, 1488
BssMI	GATC	4 cut(s)	415, 1557, 1582, 1694
BssT1I	CCWWGG	1 cut(s)	102
Bst2UI	CCWGG	2 cut(s)	910, 1756
Bst4CI	ACNGT	7 cut(s)	199, 1100, 1204, 1686, 1708, 2066, 2249
Bst6I	CTCTTC	3 cut(s)	204, 937, 1047
BstAFI	CTTAAG	1 cut(s)	278
BstAPI	GCANNNNNTGC	4 cut(s)	83, 1083, 1262, 2276
BstBAI	YACGTR	2 cut(s)	1181, 2264
BstC8I	GCNNGC	8 cut(s)	111, 471, 634, 984, 1075, 1142, 1473, 2179
BstDSI	CCRYGG	1 cut(s)	102
BstENI	CCTNNNNNAGG	1 cut(s)	1344
BstF5I	GGATG	6 cut(s)	265, 919, 1069, 1844, 2065, 2227
BstFNI	CGCG	2 cut(s)	815, 2177
BstH2I	RGCGCY	1 cut(s)	371
BstHHI	GCGC	4 cut(s)	370, 815, 1178, 1369
BstKTI	GATC	4 cut(s)	418, 1560, 1585, 1697
BstMAI	GTCTC	4 cut(s)	206, 525, 892, 1626
BstMBI	GATC	4 cut(s)	415, 1557, 1582, 1694
BstMCI	CGRYCG	2 cut(s)	418, 1488
BstNI	CCWGG	2 cut(s)	910, 1756
BstNSI	RCATGY	4 cut(s)	86, 905, 1077, 1810
BstSCI	CCNGG	5 cut(s)	792, 793, 908, 1487, 1754
BstSFI	CTRYAG	1 cut(s)	1860
BstUI	CGCG	2 cut(s)	815, 2177
BstV1I	GCAGC	8 cut(s)	343, 408, 460, 607, 1249, 1795, 1822, 2279
BstV2I	GAAGAC	1 cut(s)	1047
BstX2I	RGATCY	1 cut(s)	1694
BstYI	RGATCY	1 cut(s)	1694
Bsu15I	ATCGAT	1 cut(s)	2274
BsuRI	GGCC	6 cut(s)	227, 276, 731, 1108, 1616, 2024
BsuTUI	ATCGAT	1 cut(s)	2274
BtgI	CCRYGG	1 cut(s)	102
BtgZI	GCGATG	1 cut(s)	445
BtrI	CACGTC	3 cut(s)	1045, 1421, 1571
BtsCI	GGATG	6 cut(s)	265, 919, 1069, 1844, 2065, 2227
BtsI	GCAGTG	1 cut(s)	671
BtsIMutI	CAGTG	5 cut(s)	671, 686, 1238, 1959, 2071
BveI	ACCTGC	2 cut(s)	361, 1823
Cac8I	GCNNGC	8 cut(s)	111, 471, 634, 984, 1075, 1142, 1473, 2179
CaiI	CAGNNNCTG	1 cut(s)	1262
CciI	TCATGA	2 cut(s)	1554, 1747
CfoI	GCGC	4 cut(s)	370, 815, 1178, 1369
Cfr10I	RCCGGY	2 cut(s)	215, 2020
Cfr13I	GGNCC	4 cut(s)	274, 790, 1349, 2023
Cfr9I	CCCGGG	1 cut(s)	793
ClaI	ATCGAT	1 cut(s)	2274
CseI	GACGC	1 cut(s)	1592
Csp6I	GTAC	3 cut(s)	1393, 2037, 2261
CspAI	ACCGGT	1 cut(s)	215
CspCI	CAANNNNNGTGG	2 cut(s)	675, 710
CviQI	GTAC	3 cut(s)	1393, 2037, 2261
DpnI	GATC	4 cut(s)	417, 1559, 1584, 1696
DpnII	GATC	4 cut(s)	415, 1557, 1582, 1694
DraI	TTTAAA	1 cut(s)	640
DraIII	CACNNNGTG	1 cut(s)	188
EaeI	YGGCCR	2 cut(s)	729, 1614
Eam1104I	CTCTTC	3 cut(s)	204, 937, 1047
EarI	CTCTTC	3 cut(s)	204, 937, 1047
Ecl136II	GAGCTC	1 cut(s)	932
Eco130I	CCWWGG	1 cut(s)	102
Eco147I	AGGCCT	1 cut(s)	1108
Eco24I	GRGCYC	1 cut(s)	934
Eco31I	GGTCTC	1 cut(s)	206
Eco47I	GGWCC	2 cut(s)	790, 1349
Eco47III	AGCGCT	1 cut(s)	369
Eco53kI	GAGCTC	1 cut(s)	932
Eco57I	CTGAAG	4 cut(s)	135, 367, 1719, 2073
Eco72I	CACGTG	1 cut(s)	1181
Eco88I	CYCGRG	1 cut(s)	793
EcoICRI	GAGCTC	1 cut(s)	932
EcoNI	CCTNNNNNAGG	1 cut(s)	1344
EcoO109I	RGGNCCY	1 cut(s)	790
EcoRI	GAATTC	1 cut(s)	1726
EcoRII	CCWGG	2 cut(s)	908, 1754
EcoT14I	CCWWGG	1 cut(s)	102
EcoT22I	ATGCAT	2 cut(s)	6, 1079
EcoT38I	GRGCYC	1 cut(s)	934
ErhI	CCWWGG	1 cut(s)	102
FalI	AAGNNNNNCTT	4 cut(s)	108, 140, 2091, 2123
FaqI	GGGAC	5 cut(s)	639, 668, 776, 1177, 1412
FauI	CCCGC	2 cut(s)	616, 2272
FauNDI	CATATG	1 cut(s)	343
FblI	GTMKAC	3 cut(s)	61, 716, 1956
FokI	GGATG	6 cut(s)	252, 926, 1076, 1831, 2072, 2234
FriOI	GRGCYC	1 cut(s)	934
FspBI	CTAG	6 cut(s)	204, 645, 983, 1472, 1659, 1899
GlaI	GCGC	4 cut(s)	369, 814, 1177, 1368
GsaI	CCCAGC	1 cut(s)	469
GsuI	CTGGAG	3 cut(s)	272, 424, 1522
HaeII	RGCGCY	1 cut(s)	371
HaeIII	GGCC	6 cut(s)	227, 276, 731, 1108, 1616, 2024
HapII	CCGG	4 cut(s)	216, 794, 1488, 2021
HgaI	GACGC	1 cut(s)	1592
HhaI	GCGC	4 cut(s)	370, 815, 1178, 1369
Hin6I	GCGC	4 cut(s)	368, 813, 1176, 1367
HinP1I	GCGC	4 cut(s)	368, 813, 1176, 1367
HincII	GTYRAC	3 cut(s)	717, 1387, 1449
HindII	GTYRAC	3 cut(s)	717, 1387, 1449
HindIII	AAGCTT	1 cut(s)	2071
HinfI	GANTC	9 cut(s)	139, 145, 324, 518, 1316, 1655, 1742, 1751, 1874
HpaII	CCGG	4 cut(s)	216, 794, 1488, 2021
HphI	GGTGA	5 cut(s)	230, 359, 1010, 1565, 1735
Hpy166II	GTNNAC	9 cut(s)	62, 221, 350, 489, 717, 1129, 1387, 1449, 1957
Hpy8I	GTNNAC	9 cut(s)	62, 221, 350, 489, 717, 1129, 1387, 1449, 1957
HpyCH4III	ACNGT	7 cut(s)	199, 1100, 1204, 1686, 1708, 2066, 2249
HpyCH4IV	ACGT	8 cut(s)	491, 1044, 1180, 1420, 1570, 1984, 2115, 2263
HpySE526I	ACGT	8 cut(s)	491, 1044, 1180, 1420, 1570, 1984, 2115, 2263
HspAI	GCGC	4 cut(s)	368, 813, 1176, 1367
KflI	GGGWCCC	1 cut(s)	790
Kzo9I	GATC	4 cut(s)	415, 1557, 1582, 1694
LmnI	GCTCC	3 cut(s)	50, 106, 1295
Lsp1109I	GCAGC	8 cut(s)	343, 408, 460, 607, 1249, 1795, 1822, 2279
LweI	GCATC	8 cut(s)	88, 186, 468, 1249, 1458, 1502, 1658, 2266
MaeI	CTAG	6 cut(s)	204, 645, 983, 1472, 1659, 1899
MaeII	ACGT	8 cut(s)	491, 1044, 1180, 1420, 1570, 1984, 2115, 2263
MaeIII	GTNAC	3 cut(s)	199, 1094, 1571
MalI	GATC	4 cut(s)	417, 1559, 1584, 1696
MbiI	CCGCTC	1 cut(s)	2104
MboI	GATC	4 cut(s)	415, 1557, 1582, 1694
MboII	GAAGA	8 cut(s)	116, 221, 954, 1052, 1064, 1538, 1712, 1981
MflI	RGATCY	1 cut(s)	1694
MhlI	GDGCHC	2 cut(s)	193, 934
MlsI	TGGCCA	2 cut(s)	731, 1616
MluCI	AATT	5 cut(s)	499, 950, 1150, 1327, 1726
MluNI	TGGCCA	2 cut(s)	731, 1616
MlyI	GAGTC	4 cut(s)	139, 148, 527, 1883
MmeI	TCCRAC	2 cut(s)	400, 474
Mox20I	TGGCCA	2 cut(s)	731, 1616
Mph1103I	ATGCAT	2 cut(s)	6, 1079
MroXI	GAANNNNTTC	2 cut(s)	120, 954
MscI	TGGCCA	2 cut(s)	731, 1616
MseI	TTAA	3 cut(s)	279, 525, 639
MslI	CAYNNNNRTG	3 cut(s)	996, 1770, 1818
Msp20I	TGGCCA	2 cut(s)	731, 1616
MspA1I	CMGCKG	2 cut(s)	113, 620
MspCI	CTTAAG	1 cut(s)	278
MspI	CCGG	4 cut(s)	216, 794, 1488, 2021
MspR9I	CCNGG	5 cut(s)	794, 795, 910, 1489, 1756
Mva1269I	GAATGC	3 cut(s)	120, 975, 1514
MvaI	CCWGG	2 cut(s)	910, 1756
MvnI	CGCG	2 cut(s)	815, 2177
NciI	CCSGG	3 cut(s)	794, 795, 1489
NcoI	CCATGG	1 cut(s)	102
NdeI	CATATG	1 cut(s)	343
NdeII	GATC	4 cut(s)	415, 1557, 1582, 1694
NheI	GCTAGC	2 cut(s)	982, 1471
NlaIV	GGNNCC	6 cut(s)	108, 791, 792, 1918, 2019, 2025
NmeAIII	GCCGAG	1 cut(s)	1112
NmuCI	GTSAC	2 cut(s)	199, 1571
NsiI	ATGCAT	2 cut(s)	6, 1079
NspI	RCATGY	4 cut(s)	86, 905, 1077, 1810
OliI	CACNNNNGTG	1 cut(s)	1770
PaeI	GCATGC	1 cut(s)	1077
PagI	TCATGA	2 cut(s)	1554, 1747
PceI	AGGCCT	1 cut(s)	1108
PctI	GAATGC	3 cut(s)	120, 975, 1514
PdmI	GAANNNNTTC	2 cut(s)	120, 954
PfeI	GAWTC	5 cut(s)	324, 1316, 1655, 1742, 1751
PinAI	ACCGGT	1 cut(s)	215
Ple19I	CGATCG	1 cut(s)	418
PleI	GAGTC	4 cut(s)	139, 147, 526, 1882
PmaCI	CACGTG	1 cut(s)	1181
PmlI	CACGTG	1 cut(s)	1181
PpsI	GAGTC	4 cut(s)	139, 147, 526, 1882
Ppu21I	YACGTR	2 cut(s)	1181, 2264
PpuMI	RGGWCCY	1 cut(s)	790
Psp124BI	GAGCTC	1 cut(s)	934
Psp1406I	AACGTT	1 cut(s)	1984
Psp5II	RGGWCCY	1 cut(s)	790
Psp6I	CCWGG	2 cut(s)	908, 1754
PspCI	CACGTG	1 cut(s)	1181
PspFI	CCCAGC	1 cut(s)	465
PspGI	CCWGG	2 cut(s)	908, 1754
PspN4I	GGNNCC	6 cut(s)	108, 791, 792, 1918, 2019, 2025
PspPI	GGNCC	4 cut(s)	274, 790, 1349, 2023
PspPPI	RGGWCCY	1 cut(s)	790
PstNI	CAGNNNCTG	1 cut(s)	1262
PsuI	RGATCY	1 cut(s)	1694
PvuI	CGATCG	1 cut(s)	418
PvuII	CAGCTG	2 cut(s)	113, 620
RsaI	GTAC	3 cut(s)	1394, 2038, 2262
RsaNI	GTAC	3 cut(s)	1393, 2037, 2261
RseI	CAYNNNNRTG	3 cut(s)	996, 1770, 1818
SacI	GAGCTC	1 cut(s)	934
SalI	GTCGAC	1 cut(s)	715
SaqAI	TTAA	3 cut(s)	279, 525, 639
Sau3AI	GATC	4 cut(s)	415, 1557, 1582, 1694
Sau96I	GGNCC	4 cut(s)	274, 790, 1349, 2023
ScaI	AGTACT	1 cut(s)	1394
SchI	GAGTC	4 cut(s)	139, 148, 527, 1883
ScrFI	CCNGG	5 cut(s)	794, 795, 910, 1489, 1756
SduI	GDGCHC	2 cut(s)	193, 934
SfaNI	GCATC	8 cut(s)	88, 186, 468, 1249, 1458, 1502, 1658, 2266
SfcI	CTRYAG	1 cut(s)	1860
SinI	GGWCC	2 cut(s)	790, 1349
SmaI	CCCGGG	1 cut(s)	795
SmiMI	CAYNNNNRTG	3 cut(s)	996, 1770, 1818
SmlI	CTYRAG	2 cut(s)	278, 566
SmoI	CTYRAG	2 cut(s)	278, 566
SpeI	ACTAGT	1 cut(s)	203
SphI	GCATGC	1 cut(s)	1077
Sse9I	AATT	5 cut(s)	499, 950, 1150, 1327, 1726
SseBI	AGGCCT	1 cut(s)	1108
SspMI	CTAG	6 cut(s)	204, 645, 983, 1472, 1659, 1899
SstI	GAGCTC	1 cut(s)	934
StuI	AGGCCT	1 cut(s)	1108
StyD4I	CCNGG	5 cut(s)	792, 793, 908, 1487, 1754
StyI	CCWWGG	1 cut(s)	102
TaaI	ACNGT	7 cut(s)	199, 1100, 1204, 1686, 1708, 2066, 2249
TaiI	ACGT	8 cut(s)	494, 1047, 1183, 1423, 1573, 1987, 2118, 2266
TaqI	TCGA	5 cut(s)	716, 1581, 1942, 2138, 2274
TaqII	GACCGA	1 cut(s)	1815
TasI	AATT	5 cut(s)	499, 950, 1150, 1327, 1726
TatI	WGTACW	1 cut(s)	1392
TauI	GCSGC	3 cut(s)	1437, 2018, 2107
TfiI	GAWTC	5 cut(s)	324, 1316, 1655, 1742, 1751
Tru1I	TTAA	3 cut(s)	279, 525, 639
Tru9I	TTAA	3 cut(s)	279, 525, 639
TscAI	CASTG	5 cut(s)	678, 693, 1238, 1966, 2071
TseFI	GTSAC	2 cut(s)	199, 1571
TseI	GCWGC	8 cut(s)	356, 421, 473, 620, 1262, 1783, 1810, 2267
Tsp45I	GTSAC	2 cut(s)	199, 1571
TspGWI	ACGGA	2 cut(s)	1341, 1924
TspMI	CCCGGG	1 cut(s)	793
TspRI	CASTG	5 cut(s)	678, 693, 1238, 1966, 2071
Vha464I	CTTAAG	1 cut(s)	278
VpaK11BI	GGWCC	2 cut(s)	790, 1349
XagI	CCTNNNNNAGG	1 cut(s)	1344
XapI	RAATTY	3 cut(s)	499, 1150, 1726
XceI	RCATGY	4 cut(s)	86, 905, 1077, 1810
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1284
XmaI	CCCGGG	1 cut(s)	793
XmiI	GTMKAC	3 cut(s)	61, 716, 1956
XmnI	GAANNNNTTC	2 cut(s)	120, 954
XspI	CTAG	6 cut(s)	204, 645, 983, 1472, 1659, 1899
ZrmI	AGTACT	1 cut(s)	1394
Zsp2I	ATGCAT	2 cut(s)	6, 1079

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.