pycom17g07860

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006592
Physical Location & Seq
Forward (+)
5844676 .. 5847289
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1512 bp
ATGGCTGGTGGTTTGGATGTGTGGGAGTCTTTGGTATTTGGATATTCATTTGCTCGGTTGACCATTTTTGGCAAATTTCAGAATTTCATGTGCGATCTGGTTGCTGAGGAAATGGGGGGTTGTGTTTCGACTAGTAGTCAGAGTACTCGTAGCAGCAACAGCAACAGAGAGGCAGTGACACCCCCATGCTTGGGGATTCAATTTAGCGGTCGAAAGAGAACGAAGAAGACATTCTCTGACCATGCATATACTCTTCAGAATTTACACACCTTGCCCAACCGCATTTTTACCAACGGAAAGAGCCAGACTTCTTGCATATTCACACAGCAGGGCCGCAAAGGCATAAACCAAGATGCCATGCTTGTGTGGGAAGATTTCATGTCCGATGATGCGATATTCTGTGGTGTTTTTGACGGCCATGGTCCACACGGCCATCTTGTTGCTCGCAAAGTAAGGGATGCGTTGCCAATGAAGCTGCTATCCTTCTTGTATTCATACCAAGGGTCCAGTAAAACGTGTTTCAAAGGGAACCTAAAGAAGTCAGATTGTGGAGATACTGAGAAGGATGGTTTGGACGAGGAAAAATTGAATTTATCATGGAGAGACGCTTTCCTTAAGTCATACAAAGCCATGGACAAGGAGCTGAGGTCTCATCCTAATTTGGACTGCTTCTGCAGTGGTAGCACTGCTGTCACTCTAGTCAAACAGGGGTCAAATCTTTTCATGGGTTATATCGGGGATTCCCGAGCAATCCTGGGATCAAAGGACAGTAATGATTCCATGGTGGCAATCCAGTTAACTGTTGACTTAAAGCCTGATCTGCCAAGGGAAGCTGAAAGAATTAAACGGTGTAAGGGTAGGGTGTTTGCTTTGCATGATGAGCCTGAAGTGTCTCGAGTATGGTTGCCTTTTGATGATGCCCCTGGTTTAGCTATGGCTCGAGCCTTCGGCGATTTCTGTTTAAAGGAGTATGGAGTGATCTCAATTCCTGAGTTCTCACACCGAATACTTACTGACAGAGACCAGTTCATTGTTCTTGCTTCTGACGGGGTTTGGGATGTCTTAAGCAATGAAGAGGTTGTTGAGATAGTCTCCTCAGCCCCAACCCGGGCATCAGCAGCAAGGACTGTGGTTGACTCAGCCGCTCGCGAATGGAAACTCAAATACCCGACTTCAAAGATGGATGACTGTGCAGTCGTTTGCTTGTTTTTGGATGGGAAAATGGACTCGGAATCTGATTATGAGGAACAAGGCTTTTCATCCGCAACCCTTCAAAGCAATCACTCCGGCAATGCTGTTGAATCAGATGATGGCCAGAAGTCGGAGCCATCTTTGCAGAGGAATTTCACTGTCAGGTCATCTGATGAAAGTGATACTCATGGTAGACTACCGGTTGAGATTGAAGGGAATGAAGAAACAGTGGCAGCTGAAGAGAACTGGTTGGGTTTGGAAGGCGTGACACGCGTGAATTCCCTCGTTCAACTTCCTAGATTTTCCGAAGAAAGGCCTTAG

Protein Analysis

504

Amino Acids

55.83

Weight (kDa)

5.22

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 190 - 381 3.9e-37 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1193
AccBSI CCGCTC 1 cut(s) 1145
AccI GTMKAC 1 cut(s) 1384
AccII CGCG 2 cut(s) 1149, 1464
AciI CCGC 5 cut(s) 207, 280, 334, 1143, 1263
AclWI GGATC 1 cut(s) 766
AcoI YGGCCR 3 cut(s) 415, 430, 1312
AcsI RAATTY 6 cut(s) 74, 82, 259, 589, 1342, 1468
AcuI CTGAAG 3 cut(s) 239, 906, 1449
AfaI GTAC 1 cut(s) 145
AfiI CCNNNNNNNGG 6 cut(s) 190, 191, 1107, 1108, 1321, 1503
AflII CTTAAG 2 cut(s) 614, 1063
AflIII ACRYGT 2 cut(s) 515, 1462
AgeI ACCGGT 1 cut(s) 1390
AgsI TTSAA 8 cut(s) 200, 523, 589, 1176, 1274, 1301, 1403, 1481
AhdI GACNNNNNGTC 1 cut(s) 135
AhlI ACTAGT 1 cut(s) 131
AjnI CCWGG 2 cut(s) 753, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 5 cut(s) 475, 643, 833, 932, 1427
AluI AGCT 5 cut(s) 475, 643, 833, 932, 1427
Alw26I GTCTC 5 cut(s) 597, 654, 897, 1014, 1096
AlwI GGATC 1 cut(s) 766
Ama87I CYCGRG 4 cut(s) 744, 894, 939, 1107
AoxI GGCC 5 cut(s) 331, 415, 430, 1312, 1505
ApeKI GCWGC 4 cut(s) 153, 475, 1118, 1424
ApoI RAATTY 6 cut(s) 74, 82, 259, 589, 1342, 1468
AsiGI ACCGGT 1 cut(s) 1390
Asp700I GAANNNNTTC 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 331, 422, 504
AsuC2I CCSGG 2 cut(s) 1108, 1109
AvaI CYCGRG 4 cut(s) 744, 894, 939, 1107
AvaII GGWCC 2 cut(s) 422, 504
BalI TGGCCA 1 cut(s) 1314
BbsI GAAGAC 1 cut(s) 233
BbvCI CCTCAGC 3 cut(s) 105, 644, 1096
BbvI GCAGC 4 cut(s) 165, 462, 1130, 1436
BccI CCATC 6 cut(s) 441, 560, 1174, 1208, 1304, 1336
BceAI ACGGC 2 cut(s) 430, 445
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 2 cut(s) 755, 924
BcnI CCSGG 2 cut(s) 1108, 1109
BcoDI GTCTC 5 cut(s) 597, 654, 897, 1014, 1096
BcuI ACTAGT 1 cut(s) 131
BfaI CTAG 3 cut(s) 132, 698, 1488
BfmI CTRYAG 1 cut(s) 673
BfrI CTTAAG 2 cut(s) 614, 1063
BglI GCCNNNNNGGC 1 cut(s) 339
BisI GCNGC 6 cut(s) 154, 334, 476, 1119, 1143, 1425
BlsI GCNGC 6 cut(s) 155, 335, 477, 1120, 1144, 1426
BmcAI AGTACT 1 cut(s) 145
Bme1390I CCNGG 4 cut(s) 755, 924, 1108, 1109
Bme18I GGWCC 2 cut(s) 422, 504
BmeRI GACNNNNNGTC 1 cut(s) 135
BmeT110I CYCGRG 4 cut(s) 744, 894, 939, 1107
BmgT120I GGNCC 3 cut(s) 331, 422, 504
BmiI GGNNCC 3 cut(s) 505, 530, 1326
BmrFI CCNGG 4 cut(s) 755, 924, 1108, 1109
BmsI GCATC 5 cut(s) 343, 379, 448, 907, 1121
BpiI GAAGAC 1 cut(s) 233
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
Bpu10I CCTNAGC 3 cut(s) 105, 644, 1096
BpuMI CCSGG 2 cut(s) 1108, 1109
BsaI GGTCTC 2 cut(s) 654, 1014
BsaJI CCNNGG 8 cut(s) 418, 499, 630, 754, 780, 824, 922, 1107
BsaWI WCCGGW 1 cut(s) 1390
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 191, 1107, 1108, 1321, 1503
Bse118I RCCGGY 1 cut(s) 1390
Bse1I ACTGG 4 cut(s) 507, 793, 1024, 1442
Bse3DI GCAATG 2 cut(s) 1075, 1297
BseBI CCWGG 2 cut(s) 755, 924
BseDI CCNNGG 8 cut(s) 418, 499, 630, 754, 780, 824, 922, 1107
BseGI GGATG 8 cut(s) 22, 463, 571, 652, 1063, 1189, 1219, 1259
BseLI CCNNNNNNNGG 6 cut(s) 190, 191, 1107, 1108, 1321, 1503
BseMI GCAATG 2 cut(s) 1075, 1297
BseMII CTCAG 6 cut(s) 96, 549, 635, 981, 1110, 1152
BseNI ACTGG 4 cut(s) 507, 793, 1024, 1442
BseRI GAGGAG 1 cut(s) 1084
BseXI GCAGC 4 cut(s) 165, 462, 1130, 1436
BsgI GTGCAG 1 cut(s) 1212
Bsh1236I CGCG 2 cut(s) 1149, 1464
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 5 cut(s) 333, 417, 432, 1314, 1507
BshTI ACCGGT 1 cut(s) 1390
BsiEI CGRYCG 1 cut(s) 211
BsiHKCI CYCGRG 4 cut(s) 744, 894, 939, 1107
BsiSI CCGG 3 cut(s) 1108, 1287, 1391
BslI CCNNNNNNNGG 6 cut(s) 190, 191, 1107, 1108, 1321, 1503
BsmAI GTCTC 5 cut(s) 597, 654, 897, 1014, 1096
BsmBI CGTCTC 1 cut(s) 597
BsnI GGCC 5 cut(s) 333, 417, 432, 1314, 1507
Bso31I GGTCTC 2 cut(s) 654, 1014
BsoBI CYCGRG 4 cut(s) 744, 894, 939, 1107
Bsp143I GATC 4 cut(s) 94, 758, 817, 978
Bsp19I CCATGG 3 cut(s) 418, 630, 780
Bsp68I TCGCGA 1 cut(s) 1149
BspACI CCGC 5 cut(s) 207, 280, 334, 1143, 1263
BspANI GGCC 5 cut(s) 333, 417, 432, 1314, 1507
BspCNI CTCAG 6 cut(s) 97, 550, 636, 982, 1109, 1151
BspFNI CGCG 2 cut(s) 1149, 1464
BspLI GGNNCC 3 cut(s) 505, 530, 1326
BspMAI CTGCAG 1 cut(s) 677
BspPI GGATC 1 cut(s) 766
BspTI CTTAAG 2 cut(s) 614, 1063
BspTNI GGTCTC 2 cut(s) 654, 1014
BsrBI CCGCTC 1 cut(s) 1145
BsrDI GCAATG 2 cut(s) 1075, 1297
BsrFI RCCGGY 1 cut(s) 1390
BsrI ACTGG 4 cut(s) 507, 793, 1024, 1442
BssAI RCCGGY 1 cut(s) 1390
BssECI CCNNGG 8 cut(s) 418, 499, 630, 754, 780, 824, 922, 1107
BssMI GATC 4 cut(s) 94, 758, 817, 978
BssT1I CCWWGG 5 cut(s) 418, 499, 630, 780, 824
Bst2UI CCWGG 2 cut(s) 755, 924
Bst4CI ACNGT 7 cut(s) 770, 802, 849, 1129, 1190, 1351, 1420
Bst6I CTCTTC 3 cut(s) 258, 1068, 1425
BstAFI CTTAAG 2 cut(s) 614, 1063
BstC8I GCNNGC 2 cut(s) 445, 1147
BstDEI CTNAG 7 cut(s) 105, 558, 644, 990, 1096, 1138, 1509
BstDSI CCRYGG 3 cut(s) 418, 630, 780
BstF5I GGATG 8 cut(s) 22, 463, 571, 652, 1063, 1189, 1219, 1259
BstFNI CGCG 2 cut(s) 1149, 1464
BstKTI GATC 4 cut(s) 97, 761, 820, 981
BstMAI GTCTC 5 cut(s) 597, 654, 897, 1014, 1096
BstMBI GATC 4 cut(s) 94, 758, 817, 978
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 9 cut(s) 159, 339, 472, 681, 820, 880, 1118, 1333, 1461
BstNI CCWGG 2 cut(s) 755, 924
BstSCI CCNGG 4 cut(s) 753, 922, 1106, 1107
BstSFI CTRYAG 1 cut(s) 673
BstUI CGCG 2 cut(s) 1149, 1464
BstV1I GCAGC 4 cut(s) 165, 462, 1130, 1436
BstV2I GAAGAC 1 cut(s) 233
BsuRI GGCC 5 cut(s) 333, 417, 432, 1314, 1507
BtgI CCRYGG 3 cut(s) 418, 630, 780
BtsCI GGATG 8 cut(s) 22, 463, 571, 652, 1063, 1189, 1219, 1259
BtsI GCAGTG 3 cut(s) 180, 682, 684
BtsIMutI CAGTG 5 cut(s) 180, 682, 684, 1347, 1425
BtuMI TCGCGA 1 cut(s) 1149
Cac8I GCNNGC 2 cut(s) 445, 1147
Cfr10I RCCGGY 1 cut(s) 1390
Cfr13I GGNCC 3 cut(s) 331, 422, 504
Cfr9I CCCGGG 1 cut(s) 1107
CseI GACGC 1 cut(s) 614
Csp6I GTAC 1 cut(s) 144
CspAI ACCGGT 1 cut(s) 1390
CspCI CAANNNNNGTGG 2 cut(s) 1110, 1145
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 7 cut(s) 105, 558, 644, 990, 1096, 1138, 1509
DpnI GATC 4 cut(s) 96, 760, 819, 980
DpnII GATC 4 cut(s) 94, 758, 817, 978
DraI TTTAAA 1 cut(s) 963
DrdI GACNNNNNNGTC 1 cut(s) 1193
DriI GACNNNNNGTC 1 cut(s) 135
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 3 cut(s) 415, 430, 1312
Eam1104I CTCTTC 3 cut(s) 258, 1068, 1425
Eam1105I GACNNNNNGTC 1 cut(s) 135
EarI CTCTTC 3 cut(s) 258, 1068, 1425
Eco130I CCWWGG 5 cut(s) 418, 499, 630, 780, 824
Eco147I AGGCCT 1 cut(s) 1507
Eco31I GGTCTC 2 cut(s) 654, 1014
Eco47I GGWCC 2 cut(s) 422, 504
Eco57I CTGAAG 3 cut(s) 239, 906, 1449
Eco88I CYCGRG 4 cut(s) 744, 894, 939, 1107
EcoRI GAATTC 1 cut(s) 1468
EcoRII CCWGG 2 cut(s) 753, 922
EcoT14I CCWWGG 5 cut(s) 418, 499, 630, 780, 824
EcoT22I ATGCAT 1 cut(s) 247
ErhI CCWWGG 5 cut(s) 418, 499, 630, 780, 824
Esp3I CGTCTC 1 cut(s) 597
FblI GTMKAC 1 cut(s) 1384
Fnu4HI GCNGC 6 cut(s) 154, 334, 476, 1119, 1143, 1425
FokI GGATG 8 cut(s) 29, 470, 578, 639, 1070, 1196, 1226, 1246
Fsp4HI GCNGC 6 cut(s) 154, 334, 476, 1119, 1143, 1425
FspBI CTAG 3 cut(s) 132, 698, 1488
GluI GCNGC 6 cut(s) 154, 334, 476, 1119, 1143, 1425
HaeIII GGCC 5 cut(s) 333, 417, 432, 1314, 1507
HapII CCGG 3 cut(s) 1108, 1287, 1391
HgaI GACGC 1 cut(s) 614
HincII GTYRAC 4 cut(s) 60, 798, 805, 1135
HindII GTYRAC 4 cut(s) 60, 798, 805, 1135
HinfI GANTC 8 cut(s) 26, 196, 740, 776, 1136, 1226, 1232, 1301
HpaI GTTAAC 1 cut(s) 798
HpaII CCGG 3 cut(s) 1108, 1287, 1391
Hpy166II GTNNAC 6 cut(s) 60, 425, 798, 805, 1135, 1385
Hpy188III TCNNGA 4 cut(s) 744, 894, 989, 1148
Hpy8I GTNNAC 6 cut(s) 60, 425, 798, 805, 1135, 1385
HpyAV CCTTC 6 cut(s) 493, 556, 955, 1280, 1397, 1445
HpyCH4III ACNGT 7 cut(s) 770, 802, 849, 1129, 1190, 1351, 1420
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 6 cut(s) 245, 315, 675, 874, 1193, 1336
HpyF10VI GCNNNNNNNGC 9 cut(s) 159, 339, 472, 681, 820, 880, 1118, 1333, 1461
HpyF3I CTNAG 7 cut(s) 105, 558, 644, 990, 1096, 1138, 1509
HpySE526I ACGT 1 cut(s) 515
KspAI GTTAAC 1 cut(s) 798
Kzo9I GATC 4 cut(s) 94, 758, 817, 978
LmnI GCTCC 2 cut(s) 640, 1324
Lsp1109I GCAGC 4 cut(s) 165, 462, 1130, 1436
LweI GCATC 5 cut(s) 343, 379, 448, 907, 1121
MaeI CTAG 3 cut(s) 132, 698, 1488
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 3 cut(s) 175, 691, 1456
MalI GATC 4 cut(s) 96, 760, 819, 980
MbiI CCGCTC 1 cut(s) 1145
MboI GATC 4 cut(s) 94, 758, 817, 978
MboII GAAGA 8 cut(s) 235, 238, 245, 383, 1085, 1424, 1442, 1511
MlsI TGGCCA 1 cut(s) 1314
MluI ACGCGT 1 cut(s) 1462
MluNI TGGCCA 1 cut(s) 1314
MlyI GAGTC 3 cut(s) 35, 1130, 1220
MmeI TCCRAC 1 cut(s) 1302
MnlI CCTC 9 cut(s) 100, 163, 571, 639, 1069, 1105, 1237, 1332, 1484
Mox20I TGGCCA 1 cut(s) 1314
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 230
MscI TGGCCA 1 cut(s) 1314
MseI TTAA 6 cut(s) 615, 797, 809, 843, 962, 1064
MslI CAYNNNNRTG 2 cut(s) 184, 362
Msp20I TGGCCA 1 cut(s) 1314
MspA1I CMGCKG 1 cut(s) 1427
MspCI CTTAAG 2 cut(s) 614, 1063
MspI CCGG 3 cut(s) 1108, 1287, 1391
MspR9I CCNGG 4 cut(s) 755, 924, 1108, 1109
MvaI CCWGG 2 cut(s) 755, 924
MvnI CGCG 2 cut(s) 1149, 1464
MwoI GCNNNNNNNGC 9 cut(s) 159, 339, 472, 681, 820, 880, 1118, 1333, 1461
NciI CCSGG 2 cut(s) 1108, 1109
NcoI CCATGG 3 cut(s) 418, 630, 780
NdeII GATC 4 cut(s) 94, 758, 817, 978
NlaIV GGNNCC 3 cut(s) 505, 530, 1326
NmuCI GTSAC 3 cut(s) 175, 691, 1456
NruI TCGCGA 1 cut(s) 1149
NsiI ATGCAT 1 cut(s) 247
PaeR7I CTCGAG 2 cut(s) 894, 939
PceI AGGCCT 1 cut(s) 1507
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 5 cut(s) 196, 740, 776, 1232, 1301
PinAI ACCGGT 1 cut(s) 1390
PkrI GCNGC 6 cut(s) 155, 335, 477, 1120, 1144, 1426
PleI GAGTC 3 cut(s) 34, 1130, 1220
PpsI GAGTC 3 cut(s) 34, 1130, 1220
Psp6I CCWGG 2 cut(s) 753, 922
PspGI CCWGG 2 cut(s) 753, 922
PspN4I GGNNCC 3 cut(s) 505, 530, 1326
PspPI GGNCC 3 cut(s) 331, 422, 504
PspXI VCTCGAGB 1 cut(s) 939
PstI CTGCAG 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 1427
RruI TCGCGA 1 cut(s) 1149
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
RseI CAYNNNNRTG 2 cut(s) 184, 362
SaqAI TTAA 6 cut(s) 615, 797, 809, 843, 962, 1064
SatI GCNGC 6 cut(s) 154, 334, 476, 1119, 1143, 1425
Sau3AI GATC 4 cut(s) 94, 758, 817, 978
Sau96I GGNCC 3 cut(s) 331, 422, 504
ScaI AGTACT 1 cut(s) 145
SchI GAGTC 3 cut(s) 35, 1130, 1220
ScrFI CCNGG 4 cut(s) 755, 924, 1108, 1109
SfaNI GCATC 5 cut(s) 343, 379, 448, 907, 1121
SfcI CTRYAG 1 cut(s) 673
Sfr274I CTCGAG 2 cut(s) 894, 939
SinI GGWCC 2 cut(s) 422, 504
SlaI CTCGAG 2 cut(s) 894, 939
SmaI CCCGGG 1 cut(s) 1109
SmiMI CAYNNNNRTG 2 cut(s) 184, 362
SmlI CTYRAG 4 cut(s) 614, 894, 939, 1063
SmoI CTYRAG 4 cut(s) 614, 894, 939, 1063
SpeI ACTAGT 1 cut(s) 131
SseBI AGGCCT 1 cut(s) 1507
SsiI CCGC 5 cut(s) 207, 280, 334, 1143, 1263
SspMI CTAG 3 cut(s) 132, 698, 1488
StuI AGGCCT 1 cut(s) 1507
StyD4I CCNGG 4 cut(s) 753, 922, 1106, 1107
StyI CCWWGG 5 cut(s) 418, 499, 630, 780, 824
TaaI ACNGT 7 cut(s) 770, 802, 849, 1129, 1190, 1351, 1420
TaiI ACGT 1 cut(s) 518
TaqI TCGA 4 cut(s) 128, 211, 895, 940
TatI WGTACW 1 cut(s) 143
TauI GCSGC 2 cut(s) 336, 1145
TfiI GAWTC 5 cut(s) 196, 740, 776, 1232, 1301
Tru1I TTAA 6 cut(s) 615, 797, 809, 843, 962, 1064
Tru9I TTAA 6 cut(s) 615, 797, 809, 843, 962, 1064
TscAI CASTG 5 cut(s) 180, 682, 691, 1354, 1425
TseFI GTSAC 3 cut(s) 175, 691, 1456
TseI GCWGC 4 cut(s) 153, 475, 1118, 1424
Tsp45I GTSAC 3 cut(s) 175, 691, 1456
TspGWI ACGGA 1 cut(s) 309
TspMI CCCGGG 1 cut(s) 1107
TspRI CASTG 5 cut(s) 180, 682, 691, 1354, 1425
Vha464I CTTAAG 2 cut(s) 614, 1063
VpaK11BI GGWCC 2 cut(s) 422, 504
XapI RAATTY 6 cut(s) 74, 82, 259, 589, 1342, 1468
XhoI CTCGAG 2 cut(s) 894, 939
XmaI CCCGGG 1 cut(s) 1107
XmiI GTMKAC 1 cut(s) 1384
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 3 cut(s) 132, 698, 1488
ZrmI AGTACT 1 cut(s) 145
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.