Rorug02G0496900

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006592
Physical Location & Seq
Reverse (-)
62934686 .. 62938293
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0496900.1

Sequence Viewer

Length: 720 bp
ATGGCGGCAAAGATTTTAACGTCTCCTTTTGTAGGAATTGCCGTTTTTGTCATCTTGCTCGCTCTCTTCGCCTTTTACTATAAGCCACTCTCAAAACAGAGATTGTTCACTGTTGAAGAATTGGCTTTGTACAATGGGACTGATCAAAGTTTGCCAATCTTGTTAGGAATTCTCGGATCGGTTTTCGATGTGACGAAAGGAAAGTCTCATTACGGTGAGGGTGGGGGTTATAATCATTTTTCTGGAAGAGATGCTTCACGAGCATTTGTTTCTGGAAACTTTACAGGAGATGGGCTCACAGACTCGTTAAGTGGTTTATCCAGCCAACAGGTGAAGAGTATTGTTGAATGGAGGGATTTCTACTTTAGAACTTACACATTTGTTGGTAAGCTAGTAGGTCGGTACTATGATCCCAAAGGAAATCCTACGAAATATTTGAAGGGAGTTGAAGCAAAGGCTGCCAGAGGTGCACAGCTATTGAAGAAACAGGAGATTGAAGAAGCAAAGCAACCTGGTTGCAATTCAAGGTGGAGTCAGGATGAGGGCGGAGAGGTTTGGTGTGATGTGGGTGTCCCACGATTAGTTCAGAGGCCACTAGAAATTGCTTTGACTGGGAAAATGAGCAAGCGATGTGCGTGCTTTAAAGAGGATCAACTGAGCCAACCAGGGTTGGAAGTCTACGAAGGATGTGATTATCTTGCCAAGACCTGCCGAGTTTAA

Protein Analysis

239

Amino Acids

26.44

Weight (kDa)

8.79

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 37 - 97 1.9e-07 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 678
AciI CCGC 2 cut(s) 5, 546
AclWI GGATC 3 cut(s) 184, 404, 657
AcsI RAATTY 1 cut(s) 168
AfaI GTAC 2 cut(s) 131, 404
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 7 cut(s) 116, 347, 439, 449, 481, 497, 525
AjnI CCWGG 2 cut(s) 511, 664
AluBI AGCT 2 cut(s) 391, 475
AluI AGCT 2 cut(s) 391, 475
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 2 cut(s) 27, 210
Alw44I GTGCAC 1 cut(s) 468
AlwI GGATC 3 cut(s) 184, 404, 657
AoxI GGCC 1 cut(s) 590
ApaLI GTGCAC 1 cut(s) 468
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 1 cut(s) 168
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AsuHPI GGTGA 2 cut(s) 227, 343
BaeGI GKGCMC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 1 cut(s) 445
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 618, 652
BciT130I CCWGG 2 cut(s) 513, 666
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 27, 210
BfaI CTAG 2 cut(s) 392, 596
BfuAI ACCTGC 1 cut(s) 716
BisI GCNGC 2 cut(s) 6, 459
BlsI GCNGC 2 cut(s) 7, 460
Bme1390I CCNGG 2 cut(s) 513, 666
BmrFI CCNGG 2 cut(s) 513, 666
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 621
BplI GAGNNNNNCTC 2 cut(s) 279, 311
BsaJI CCNNGG 1 cut(s) 665
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 616
BseBI CCWGG 2 cut(s) 513, 666
BseDI CCNNGG 1 cut(s) 665
BseGI GGATG 2 cut(s) 544, 692
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 1 cut(s) 616
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 1 cut(s) 445
BshFI GGCC 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 472
BslFI GGGAC 2 cut(s) 151, 557
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 2 cut(s) 27, 210
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 151, 557
BsnI GGCC 1 cut(s) 592
Bsp1286I GDGCHC 2 cut(s) 297, 472
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 4 cut(s) 142, 176, 409, 649
BspACI CCGC 2 cut(s) 5, 546
BspANI GGCC 1 cut(s) 592
BspCNI CTCAG 1 cut(s) 648
BspMI ACCTGC 1 cut(s) 716
BspPI GGATC 3 cut(s) 184, 404, 657
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 1 cut(s) 616
BssECI CCNNGG 1 cut(s) 665
BssMI GATC 4 cut(s) 142, 176, 409, 649
BssSI CACGAG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 2 cut(s) 513, 666
Bst4CI ACNGT 2 cut(s) 112, 215
Bst6I CTCTTC 3 cut(s) 71, 241, 329
BstAPI GCANNNNNTGC 1 cut(s) 458
BstAUI TGTACA 1 cut(s) 129
BstC8I GCNNGC 3 cut(s) 60, 626, 637
BstDEI CTNAG 1 cut(s) 656
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 544, 692
BstKTI GATC 4 cut(s) 145, 179, 412, 652
BstMAI GTCTC 2 cut(s) 27, 210
BstMBI GATC 4 cut(s) 142, 176, 409, 649
BstMWI GCNNNNNNNGC 4 cut(s) 68, 260, 458, 467
BstNI CCWGG 2 cut(s) 513, 666
BstSCI CCNGG 2 cut(s) 511, 664
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 1 cut(s) 445
BsuRI GGCC 1 cut(s) 592
BtgZI GCGATG 1 cut(s) 643
BtsCI GGATG 2 cut(s) 544, 692
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 3 cut(s) 60, 626, 637
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 2 cut(s) 130, 403
CviJI RGCY 9 cut(s) 85, 125, 295, 324, 391, 458, 475, 592, 660
CviKI_1 RGCY 9 cut(s) 85, 125, 295, 324, 391, 458, 475, 592, 660
CviQI GTAC 2 cut(s) 130, 403
DdeI CTNAG 1 cut(s) 656
DpnI GATC 4 cut(s) 144, 178, 411, 651
DpnII GATC 4 cut(s) 142, 176, 409, 649
DraI TTTAAA 1 cut(s) 643
Eam1104I CTCTTC 3 cut(s) 71, 241, 329
EarI CTCTTC 3 cut(s) 71, 241, 329
EciI GGCGGA 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 297
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 2 cut(s) 511, 664
EcoT38I GRGCYC 1 cut(s) 297
Esp3I CGTCTC 1 cut(s) 27
FaiI YATR 3 cut(s) 81, 231, 408
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 2 cut(s) 151, 557
FbaI TGATCA 1 cut(s) 142
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 2 cut(s) 6, 459
FokI GGATG 2 cut(s) 551, 699
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 6, 459
FspBI CTAG 2 cut(s) 392, 596
GluI GCNGC 2 cut(s) 6, 459
HaeIII GGCC 1 cut(s) 592
HinfI GANTC 2 cut(s) 302, 532
HphI GGTGA 2 cut(s) 227, 343
Hpy166II GTNNAC 3 cut(s) 108, 470, 679
Hpy188I TCNGA 2 cut(s) 176, 588
Hpy188III TCNNGA 4 cut(s) 243, 258, 273, 536
Hpy8I GTNNAC 3 cut(s) 108, 470, 679
HpyAV CCTTC 2 cut(s) 433, 677
HpyCH4III ACNGT 2 cut(s) 112, 215
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 2 cut(s) 470, 519
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 260, 458, 467
HpyF3I CTNAG 1 cut(s) 656
HpySE526I ACGT 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 142, 176, 409, 649
Lsp1109I GCAGC 1 cut(s) 445
LweI GCATC 1 cut(s) 241
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 2 cut(s) 392, 596
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 190
MalI GATC 4 cut(s) 144, 178, 411, 651
MboI GATC 4 cut(s) 142, 176, 409, 649
MboII GAAGA 6 cut(s) 58, 128, 258, 346, 493, 509
MhlI GDGCHC 2 cut(s) 297, 472
MluCI AATT 5 cut(s) 36, 119, 168, 520, 600
MlyI GAGTC 2 cut(s) 296, 541
MmeI TCCRAC 1 cut(s) 651
MnlI CCTC 7 cut(s) 211, 345, 458, 535, 544, 582, 640
MseI TTAA 4 cut(s) 17, 308, 642, 718
MslI CAYNNNNRTG 1 cut(s) 213
MspR9I CCNGG 2 cut(s) 513, 666
MvaI CCWGG 2 cut(s) 513, 666
MwoI GCNNNNNNNGC 4 cut(s) 68, 260, 458, 467
NdeII GATC 4 cut(s) 142, 176, 409, 649
NmuCI GTSAC 1 cut(s) 190
PkrI GCNGC 2 cut(s) 7, 460
PleI GAGTC 2 cut(s) 296, 540
PpsI GAGTC 2 cut(s) 296, 540
PsiI TTATAA 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 511, 664
PspGI CCWGG 2 cut(s) 511, 664
RsaI GTAC 2 cut(s) 131, 404
RsaNI GTAC 2 cut(s) 130, 403
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 4 cut(s) 17, 308, 642, 718
SatI GCNGC 2 cut(s) 6, 459
Sau3AI GATC 4 cut(s) 142, 176, 409, 649
SchI GAGTC 2 cut(s) 296, 541
ScrFI CCNGG 2 cut(s) 513, 666
SduI GDGCHC 2 cut(s) 297, 472
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 5 cut(s) 36, 119, 168, 520, 600
SsiI CCGC 2 cut(s) 5, 546
SspI AATATT 1 cut(s) 434
SspMI CTAG 2 cut(s) 392, 596
StyD4I CCNGG 2 cut(s) 511, 664
TaaI ACNGT 2 cut(s) 112, 215
TaiI ACGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 186
TasI AATT 5 cut(s) 36, 119, 168, 520, 600
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 4 cut(s) 17, 308, 642, 718
Tru9I TTAA 4 cut(s) 17, 308, 642, 718
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 190
TspRI CASTG 1 cut(s) 115
VneI GTGCAC 1 cut(s) 468
XagI CCTNNNNNAGG 1 cut(s) 30
XapI RAATTY 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 678
XspI CTAG 2 cut(s) 392, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.