RchiOBHm_Chr2g0162371

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006592
Physical Location & Seq
Reverse (-)
78077345 .. 78081929
4585 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53065

Sequence Viewer

Length: 1413 bp
ATGGGGGGCTGTGTTTCGACTGGTAATAAAAGGACTTGCAGTAGCAGGAACAATGGAGAGACGCTTTCTCCTTCATGCTTGGGGATTGGATTTTGTGCGAAAAAGAGGCCAAAGACATTCTCGGACCGTGTATATACCCTGCAGAGTTTACCTTCCATACCCAACCGCATTTTTGCCAATGGAAAGAGCAGAGCGTCTTGCATATTCACGCAGCAGGGCCGTAAAGGCATAAACCAGGATGCCATGGTTGTGTGGGAAGACTTCATGTCAGAAGATGCGATATTTTGTGGTGTGTTTGATGGCCACGGTCCACACGGTCATCTTGTTGCTCGCAAAGTAAGGGATGCATTGCCATTAAAGCTGCTATCCTTCTTGAATTCTTCTCAATCAAGGTCAAATGTGTCGAGTAAAACATGTTTCAGAGGGAATGTAAAGAAACCAGATGGTGGAGAAACTGAGAAGGATGCTTCACCTGAGGAGAAATTAAATCTAGAATGGAGAGAAGCTTTCATGAAGTCATACAAGTCCATGGACAAGGAACTTAGGTCTCATCCAAATGTAGATTGCTTCTGTAGTGGTAGCACTGCTGTCACTCTAGTCAAACAGGGGTCAAATCTTTTCATGGGTTATATTGGGGATTCCCGAGCAATCCTTGGATCAAAAGACAGTAGCGATTCCATGATAGCAATCCAGTTAACTGTTGATCTAAAGCCTGATCTGCCAAGGGAAGCTGAAAGGATTAAACGATGTAAGGGTAGGGTGTTTGCTTTGCAAGATGAGCCTGAAGTACCTCGAGTGTGGTTGCCTTTTGATGATGCTCCTGGTTTAGCAATGGCTCGAGCCTTTGGGGATTTCTGTTTGAAGGAGTATGGAGTGATCTCCATTCCTGAATTTGCACACCGGATGCTTACAGAGCGAGACCAGTTCATTGTCCTTGCCTCAGATGGGGTTTGGGATGTCTTGAGCAATGAAGAGGTTGTTGAGATAATTTCCTCAGCTCCAAGCCGCTCAGCAGCTTCAAGATATGTGGTAGATGCAGCTGCTCGTGAATGGAAACTTAAATACCCAACGTCAAAGATGGACGATTGTGCAGTTGTTTGCTTGTTTTTAGATGGGAAAATGGATTCAGAATCTGATTACGAGGAACAAGGCTTTTCATCTGCAACCCTCCAGAGCAATCACTCCGGCAATGCAGTTGAATCCGATGATGGCCAGAAGTCTGAGCCCTCTTTGCAGAGGAATTGCACTGTAAGATCATCTGATGAAAATGATACTTATGGACGACTACCTGTTGAGAATGAAGGGAATGTCGAACCAATGTCAACTGAAGACCAGAATTGGTTAGGTTTGGAAGGTGTCACGCGTGTGAACTCCCTGGTTCAGCTTCCAAGATTTTCTGAGGAAAGGCCATAA

Protein Analysis

470

Amino Acids

52.01

Weight (kDa)

5.45

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 160 - 346 1.4e-35 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 446
AccBSI CCGCTC 1 cut(s) 1008
AccII CGCG 1 cut(s) 1363
AciI CCGC 2 cut(s) 166, 1006
AclWI GGATC 1 cut(s) 664
AcoI YGGCCR 2 cut(s) 301, 1210
AcsI RAATTY 2 cut(s) 376, 890
AcuI CTGAAG 2 cut(s) 804, 1347
AfaI GTAC 1 cut(s) 789
AfiI CCNNNNNNNGG 2 cut(s) 446, 945
AflIII ACRYGT 2 cut(s) 413, 1361
AgsI TTSAA 4 cut(s) 376, 862, 1020, 1199
AhdI GACNNNNNGTC 1 cut(s) 265
AjnI CCWGG 3 cut(s) 234, 820, 1374
AleI CACNNNNGTG 1 cut(s) 1364
AluBI AGCT 7 cut(s) 361, 506, 731, 998, 1016, 1040, 1384
AluI AGCT 7 cut(s) 361, 506, 731, 998, 1016, 1040, 1384
Alw26I GTCTC 3 cut(s) 53, 552, 912
AlwI GGATC 1 cut(s) 664
AlwNI CAGNNNCTG 1 cut(s) 1133
Ama87I CYCGRG 3 cut(s) 642, 792, 837
AoxI GGCC 5 cut(s) 107, 217, 301, 1210, 1406
ApeKI GCWGC 5 cut(s) 211, 361, 1013, 1037, 1040
ApoI RAATTY 2 cut(s) 376, 890
AspS9I GGNCC 3 cut(s) 124, 217, 308
AsuHPI GGTGA 1 cut(s) 462
AvaI CYCGRG 3 cut(s) 642, 792, 837
AvaII GGWCC 2 cut(s) 124, 308
AxyI CCTNAGG 1 cut(s) 474
BalI TGGCCA 2 cut(s) 303, 1212
BanII GRGCYC 1 cut(s) 1227
BauI CACGAG 1 cut(s) 1044
BbsI GAAGAC 2 cut(s) 264, 1335
BbvCI CCTCAGC 1 cut(s) 994
BbvI GCAGC 5 cut(s) 223, 348, 1025, 1027, 1049
BccI CCATC 6 cut(s) 293, 437, 938, 1072, 1106, 1202
BceAI ACGGC 1 cut(s) 204
BciT130I CCWGG 3 cut(s) 236, 822, 1376
BcoDI GTCTC 3 cut(s) 53, 552, 912
BfaI CTAG 2 cut(s) 491, 596
BfmI CTRYAG 2 cut(s) 140, 571
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 6 cut(s) 212, 362, 1006, 1014, 1038, 1041
BlpI GCTNAGC 1 cut(s) 1009
BlsI GCNGC 6 cut(s) 213, 363, 1007, 1015, 1039, 1042
Bme1390I CCNGG 3 cut(s) 236, 822, 1376
Bme18I GGWCC 2 cut(s) 124, 308
BmeRI GACNNNNNGTC 1 cut(s) 265
BmeT110I CYCGRG 3 cut(s) 642, 792, 837
BmgT120I GGNCC 3 cut(s) 124, 217, 308
BmrFI CCNGG 3 cut(s) 236, 822, 1376
BmsI GCATC 7 cut(s) 229, 265, 334, 454, 805, 894, 1024
BpiI GAAGAC 2 cut(s) 264, 1335
BpmI CTGGAG 1 cut(s) 1154
Bpu10I CCTNAGC 1 cut(s) 994
Bpu1102I GCTNAGC 1 cut(s) 1009
BpuEI CTTGAG 1 cut(s) 982
BsaBI GATNNNNATC 1 cut(s) 686
BsaI GGTCTC 2 cut(s) 552, 912
BsaJI CCNNGG 6 cut(s) 243, 304, 528, 652, 722, 1374
BsaWI WCCGGW 1 cut(s) 900
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 945
Bse1I ACTGG 3 cut(s) 25, 691, 922
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 4 cut(s) 347, 837, 973, 1195
Bse8I GATNNNNATC 1 cut(s) 686
BseBI CCWGG 3 cut(s) 236, 822, 1376
BseDI CCNNGG 6 cut(s) 243, 304, 528, 652, 722, 1374
BseGI GGATG 6 cut(s) 244, 349, 469, 550, 909, 961
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 2 cut(s) 446, 945
BseMI GCAATG 4 cut(s) 347, 837, 973, 1195
BseMII CTCAG 7 cut(s) 447, 465, 954, 1008, 1023, 1212, 1389
BseNI ACTGG 3 cut(s) 25, 691, 922
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 5 cut(s) 223, 348, 1025, 1027, 1049
BsgI GTGCAG 1 cut(s) 1110
Bsh1236I CGCG 1 cut(s) 1363
BshFI GGCC 5 cut(s) 109, 219, 303, 1212, 1408
BsiHKCI CYCGRG 3 cut(s) 642, 792, 837
BsiSI CCGG 2 cut(s) 901, 1185
BslI CCNNNNNNNGG 2 cut(s) 446, 945
BsmAI GTCTC 3 cut(s) 53, 552, 912
BsmBI CGTCTC 1 cut(s) 53
BsnI GGCC 5 cut(s) 109, 219, 303, 1212, 1408
Bso31I GGTCTC 2 cut(s) 552, 912
BsoBI CYCGRG 3 cut(s) 642, 792, 837
Bsp1286I GDGCHC 1 cut(s) 1227
Bsp143I GATC 5 cut(s) 656, 703, 715, 876, 1253
Bsp1720I GCTNAGC 1 cut(s) 1009
Bsp19I CCATGG 2 cut(s) 243, 528
BspACI CCGC 2 cut(s) 166, 1006
BspANI GGCC 5 cut(s) 109, 219, 303, 1212, 1408
BspCNI CTCAG 7 cut(s) 448, 466, 953, 1007, 1022, 1213, 1390
BspFNI CGCG 1 cut(s) 1363
BspHI TCATGA 1 cut(s) 510
BspMAI CTGCAG 1 cut(s) 144
BspPI GGATC 1 cut(s) 664
BspTNI GGTCTC 2 cut(s) 552, 912
BsrBI CCGCTC 1 cut(s) 1008
BsrDI GCAATG 4 cut(s) 347, 837, 973, 1195
BsrI ACTGG 3 cut(s) 25, 691, 922
BssECI CCNNGG 6 cut(s) 243, 304, 528, 652, 722, 1374
BssMI GATC 5 cut(s) 656, 703, 715, 876, 1253
BssSI CACGAG 1 cut(s) 1044
BssT1I CCWWGG 4 cut(s) 243, 528, 652, 722
Bst2BI CACGAG 1 cut(s) 1044
Bst2UI CCWGG 3 cut(s) 236, 822, 1376
Bst4CI ACNGT 6 cut(s) 128, 308, 317, 668, 700, 1249
Bst6I CTCTTC 1 cut(s) 966
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 8 cut(s) 456, 474, 542, 940, 994, 1009, 1221, 1398
BstDSI CCRYGG 3 cut(s) 243, 304, 528
BstF5I GGATG 6 cut(s) 244, 349, 469, 550, 909, 961
BstFNI CGCG 1 cut(s) 1363
BstKTI GATC 5 cut(s) 659, 706, 718, 879, 1256
BstMAI GTCTC 3 cut(s) 53, 552, 912
BstMBI GATC 5 cut(s) 656, 703, 715, 876, 1253
BstMWI GCNNNNNNNGC 6 cut(s) 225, 358, 718, 778, 913, 1231
BstNI CCWGG 3 cut(s) 236, 822, 1376
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 3 cut(s) 234, 820, 1374
BstSFI CTRYAG 2 cut(s) 140, 571
BstUI CGCG 1 cut(s) 1363
BstV1I GCAGC 5 cut(s) 223, 348, 1025, 1027, 1049
BstV2I GAAGAC 2 cut(s) 264, 1335
Bsu36I CCTNAGG 1 cut(s) 474
BsuRI GGCC 5 cut(s) 109, 219, 303, 1212, 1408
BtgI CCRYGG 3 cut(s) 243, 304, 528
BtsCI GGATG 6 cut(s) 244, 349, 469, 550, 909, 961
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 582, 1245
Cac8I GCNNGC 1 cut(s) 331
CaiI CAGNNNCTG 1 cut(s) 1133
CciI TCATGA 1 cut(s) 510
Cfr13I GGNCC 3 cut(s) 124, 217, 308
CpoI CGGWCCG 1 cut(s) 124
CseI GACGC 2 cut(s) 70, 183
Csp6I GTAC 1 cut(s) 788
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CspI CGGWCCG 1 cut(s) 124
CviAII CATG 8 cut(s) 75, 244, 265, 414, 511, 529, 622, 679
CviQI GTAC 1 cut(s) 788
DdeI CTNAG 8 cut(s) 456, 474, 542, 940, 994, 1009, 1221, 1398
DpnI GATC 5 cut(s) 658, 705, 717, 878, 1255
DpnII GATC 5 cut(s) 656, 703, 715, 876, 1253
DriI GACNNNNNGTC 1 cut(s) 265
EaeI YGGCCR 2 cut(s) 301, 1210
Eam1104I CTCTTC 1 cut(s) 966
Eam1105I GACNNNNNGTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 966
Eco130I CCWWGG 4 cut(s) 243, 528, 652, 722
Eco24I GRGCYC 1 cut(s) 1227
Eco31I GGTCTC 2 cut(s) 552, 912
Eco47I GGWCC 2 cut(s) 124, 308
Eco57I CTGAAG 2 cut(s) 804, 1347
Eco81I CCTNAGG 1 cut(s) 474
Eco88I CYCGRG 3 cut(s) 642, 792, 837
EcoRI GAATTC 1 cut(s) 376
EcoRII CCWGG 3 cut(s) 234, 820, 1374
EcoT14I CCWWGG 4 cut(s) 243, 528, 652, 722
EcoT22I ATGCAT 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 1227
ErhI CCWWGG 4 cut(s) 243, 528, 652, 722
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 8 cut(s) 78, 247, 268, 417, 514, 532, 625, 682
FatI CATG 8 cut(s) 74, 243, 264, 413, 510, 528, 621, 678
Fnu4HI GCNGC 6 cut(s) 212, 362, 1006, 1014, 1038, 1041
FokI GGATG 6 cut(s) 251, 356, 476, 537, 916, 968
FriOI GRGCYC 1 cut(s) 1227
Fsp4HI GCNGC 6 cut(s) 212, 362, 1006, 1014, 1038, 1041
FspBI CTAG 2 cut(s) 491, 596
GluI GCNGC 6 cut(s) 212, 362, 1006, 1014, 1038, 1041
GsuI CTGGAG 1 cut(s) 1154
HaeIII GGCC 5 cut(s) 109, 219, 303, 1212, 1408
HapII CCGG 2 cut(s) 901, 1185
HgaI GACGC 2 cut(s) 70, 183
Hin1II CATG 8 cut(s) 78, 247, 268, 417, 514, 532, 625, 682
HincII GTYRAC 2 cut(s) 696, 1323
HindII GTYRAC 2 cut(s) 696, 1323
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 5 cut(s) 638, 674, 1124, 1130, 1199
HpaI GTTAAC 1 cut(s) 696
HpaII CCGG 2 cut(s) 901, 1185
HphI GGTGA 1 cut(s) 462
Hpy166II GTNNAC 5 cut(s) 149, 311, 696, 1323, 1369
Hpy188III TCNNGA 9 cut(s) 373, 491, 511, 642, 887, 961, 1020, 1046, 1171
Hpy8I GTNNAC 5 cut(s) 149, 311, 696, 1323, 1369
HpyAV CCTTC 7 cut(s) 81, 162, 379, 454, 856, 1295, 1346
HpyCH4III ACNGT 6 cut(s) 128, 308, 317, 668, 700, 1249
HpyCH4IV ACGT 1 cut(s) 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 225, 358, 718, 778, 913, 1231
HpyF3I CTNAG 8 cut(s) 456, 474, 542, 940, 994, 1009, 1221, 1398
HpySE526I ACGT 1 cut(s) 1070
Hsp92II CATG 8 cut(s) 78, 247, 268, 417, 514, 532, 625, 682
KspAI GTTAAC 1 cut(s) 696
Kzo9I GATC 5 cut(s) 656, 703, 715, 876, 1253
LmnI GCTCC 2 cut(s) 823, 1003
Lsp1109I GCAGC 5 cut(s) 223, 348, 1025, 1027, 1049
LweI GCATC 7 cut(s) 229, 265, 334, 454, 805, 894, 1024
MaeI CTAG 2 cut(s) 491, 596
MaeII ACGT 1 cut(s) 1070
MaeIII GTNAC 2 cut(s) 589, 1357
MalI GATC 5 cut(s) 658, 705, 717, 878, 1255
MbiI CCGCTC 1 cut(s) 1008
MboI GATC 5 cut(s) 656, 703, 715, 876, 1253
MboII GAAGA 5 cut(s) 269, 284, 372, 983, 1340
MhlI GDGCHC 1 cut(s) 1227
MlsI TGGCCA 2 cut(s) 303, 1212
MluCI AATT 6 cut(s) 376, 482, 890, 987, 1240, 1336
MluI ACGCGT 1 cut(s) 1361
MluNI TGGCCA 2 cut(s) 303, 1212
Mox20I TGGCCA 2 cut(s) 303, 1212
Mph1103I ATGCAT 1 cut(s) 349
MscI TGGCCA 2 cut(s) 303, 1212
MseI TTAA 5 cut(s) 356, 485, 695, 741, 1059
MslI CAYNNNNRTG 3 cut(s) 248, 555, 1364
Msp20I TGGCCA 2 cut(s) 303, 1212
MspA1I CMGCKG 1 cut(s) 1040
MspI CCGG 2 cut(s) 901, 1185
MspR9I CCNGG 3 cut(s) 236, 822, 1376
MvaI CCWGG 3 cut(s) 236, 822, 1376
MvnI CGCG 1 cut(s) 1363
MwoI GCNNNNNNNGC 6 cut(s) 225, 358, 718, 778, 913, 1231
NcoI CCATGG 2 cut(s) 243, 528
NdeII GATC 5 cut(s) 656, 703, 715, 876, 1253
NlaIII CATG 8 cut(s) 78, 247, 268, 417, 514, 532, 625, 682
NmuCI GTSAC 2 cut(s) 589, 1357
NsiI ATGCAT 1 cut(s) 349
NspI RCATGY 1 cut(s) 417
OliI CACNNNNGTG 1 cut(s) 1364
PaeR7I CTCGAG 2 cut(s) 792, 837
PagI TCATGA 1 cut(s) 510
PciI ACATGT 1 cut(s) 413
PfeI GAWTC 5 cut(s) 638, 674, 1124, 1130, 1199
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 6 cut(s) 213, 363, 1007, 1015, 1039, 1042
PscI ACATGT 1 cut(s) 413
Psp6I CCWGG 3 cut(s) 234, 820, 1374
PspGI CCWGG 3 cut(s) 234, 820, 1374
PspPI GGNCC 3 cut(s) 124, 217, 308
PspXI VCTCGAGB 2 cut(s) 792, 837
PstI CTGCAG 1 cut(s) 144
PstNI CAGNNNCTG 1 cut(s) 1133
PvuII CAGCTG 1 cut(s) 1040
RsaI GTAC 1 cut(s) 789
RsaNI GTAC 1 cut(s) 788
RseI CAYNNNNRTG 3 cut(s) 248, 555, 1364
Rsr2I CGGWCCG 1 cut(s) 124
RsrII CGGWCCG 1 cut(s) 124
SaqAI TTAA 5 cut(s) 356, 485, 695, 741, 1059
SatI GCNGC 6 cut(s) 212, 362, 1006, 1014, 1038, 1041
Sau3AI GATC 5 cut(s) 656, 703, 715, 876, 1253
Sau96I GGNCC 3 cut(s) 124, 217, 308
ScrFI CCNGG 3 cut(s) 236, 822, 1376
SduI GDGCHC 1 cut(s) 1227
SfaNI GCATC 7 cut(s) 229, 265, 334, 454, 805, 894, 1024
SfcI CTRYAG 2 cut(s) 140, 571
Sfr274I CTCGAG 2 cut(s) 792, 837
SinI GGWCC 2 cut(s) 124, 308
SlaI CTCGAG 2 cut(s) 792, 837
SmiMI CAYNNNNRTG 3 cut(s) 248, 555, 1364
SmlI CTYRAG 3 cut(s) 792, 837, 961
SmoI CTYRAG 3 cut(s) 792, 837, 961
Sse9I AATT 6 cut(s) 376, 482, 890, 987, 1240, 1336
SsiI CCGC 2 cut(s) 166, 1006
SspMI CTAG 2 cut(s) 491, 596
StyD4I CCNGG 3 cut(s) 234, 820, 1374
StyI CCWWGG 4 cut(s) 243, 528, 652, 722
TaaI ACNGT 6 cut(s) 128, 308, 317, 668, 700, 1249
TaiI ACGT 1 cut(s) 1073
TaqI TCGA 5 cut(s) 17, 404, 793, 838, 1311
TasI AATT 6 cut(s) 376, 482, 890, 987, 1240, 1336
TauI GCSGC 1 cut(s) 1008
TfiI GAWTC 5 cut(s) 638, 674, 1124, 1130, 1199
Tru1I TTAA 5 cut(s) 356, 485, 695, 741, 1059
Tru9I TTAA 5 cut(s) 356, 485, 695, 741, 1059
TscAI CASTG 2 cut(s) 589, 1252
TseFI GTSAC 2 cut(s) 589, 1357
TseI GCWGC 5 cut(s) 211, 361, 1013, 1037, 1040
Tsp45I GTSAC 2 cut(s) 589, 1357
TspRI CASTG 2 cut(s) 589, 1252
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 2 cut(s) 124, 308
XapI RAATTY 2 cut(s) 376, 890
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 1 cut(s) 417
XhoI CTCGAG 2 cut(s) 792, 837
XspI CTAG 2 cut(s) 491, 596
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.