Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0006592

Physical Location & Seq

Genomic Coordinates Forward (+)

62949969 .. 62959530

Gene Structure 9562 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0497300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGACAAGTCCAGCGCTCTCGAGTACATCAACCAGATGTTCCCCACAGAGGCATCGTTATCCGGCGTCGAGCCGCTAATGCAGAAGATTCACAGTGAAATTCGGCGCGTTGATGCTGGAATTTTAGCTGCCGTTCGTCAACAGAGCAATTCAGGGACCAAAGCCAAGGAGGATCTTGCTGCCGCTACGCGAGCCGTGGAGGAACTCATGTATAAGATCCGGGAAATCAAAACAAAGGCTGAGCAGAGTGAGACAATGGTTCAGGAGATATGTCGTGACATTAAGAAGTTGGATTTTGCTAAGAAGCATATAACAACCACCATTACTGCTCTTCATCGTCTTACCATGCTCGTCTCTGCTGTTGAACAGCTTCAAGTGATGGCTTCCAAACGACAATATAAAGAGGCGGCTGCACAGTTGGAGGCTGTGAACCAATTATGTAGCCATTTTGAAGCCTATAGGGATGTTCCAAAAATCACAGAGCTCAGGGAGAAATTTAAGAATATCAAACAAATATTAAAGTCACACGTGTTTTCCGATTTCTCAAGCTTAGGTACTGGAAAGGAGTCGGAAGAATCTAATTTACTCCAGCAGCTATCTGAGGCTTGCTTAGTTGTTGATGCATTAGAGGCATCTGTGAGGGAAGAATTAGTAAATAACTTTTGCAGCAGGGAGCTTACTTCTTATGAGCAAATATTTGAAGGAGCTGAACTAGCTAAGTTGGATAAGACTGAACGAAGATATGCATGGATCAAGCGCCGCATGAGAACGAATGAAGAAATCTGGAAAATCTTTCCTTCTTCATGGCATGTTTCTTACCGACTCTGCATTCAGTTCTGCAAAAAAACAAGGAAGCAGCTAGAAGATATCCTTAATAATCAGAAAGAAAAGCCAGATGTTGGGACATTGCTGATGGCACTACAGCGAACTCTTGAATTTGAGGATGAATTGGCAGAAAAATTTGGAGGTGGTACTCGAGGCAGGGAGGTTGGAAATGAGATTGAAGAAATTGGTAGAGGAGAAAACACTACTCAAAGTGCTTCAGATATTCGAAAGAAATATGAAAAGAAGCTTGCTGCCCATCAAGGAAATGCCACAGAGGAAAAGGATAAAGAGTTATCTGTGCCTGGAGCTGGGTTCAACTTCCGTGGAATCATCTCATCTTGCTTCGAGCCTCACTTGACTGTTTACACAGAACTAGAAGAGAAGACACTAATGGAGAATTTGGAAAAACTTGTTCAGGAGGAGACATGGGATGTTGAGGAGGGAAGTCAGAATAATGTTTTGTCAAGTAGCATGCAGTTGTTTCTTATAATCAAGAGGAGCTTAAAACGGTGCAGTGCTTTAACAAAGAATCAGACATTATTTAACTTGTTCAAGGTGTTTCAAAGGATTCTCAAAGCATATGCAACAAAGCTTTTTATAAGATTACCTAAAGGCGGTACAGGAATTGTTGCAGCAGCAACTGGTATGGATGGACAGATAAAGACATCAGACAGAGATGAGAGGGTGATTTGTTACATTGTCAATTCAGCGGAATATTGCACCAACACGTCTGGTGAACTCGCTGAGAGTGTGTCCAAAATAATTGATTCTCAATTCGCAGATGGGGTGGATATGTCGGAAGTGCAGGATGAATTTTCAGCAGTTATAACAAGAGCATTGGTAACCTTGGTGCATGGCCTAGAAACCAAGTTTGACAATGAAATGGCTGCAATGACACGAGTTCCATGGGGTACACTTGAAAGTGTTGGTGACCAATCAGAGTATGTTAATGGCATAAATATGATCCTTGCTAGTAGTATACCTATACTTGGAAGCCTTCTTTCACCGATCTACTTCCAATTCTTCTTGGACAAGTTGGCATCATCTCTTGGTCCACGTTTCTATGCTAACATTTTCAAGTGCAAGCAAATATCAGAGACTGGAGCTCAACAGATGCTATTGGATACTCAAGCTGTGAAGACTATTCTTTTGGATATCCCATCACTTGGTCACCAGACGTCACGTGCTGCAAGCTATGCAAAATTTGTAAGCCGTGAGATGAGCAAAGCTGAAGCACTTCTAAAGGTCATACTGTCCCCAATTGATTCTGTCGCAGATACATATCGTGCATTGCTACCAGAGGGAACACCTATGGAGTTTCAGCGAATTCTTGAGCTCAAGGGTCTTAAAAAGGCAGATCAGCAGAGTATACTTGAAGACTTCAACAAGCATGGGCCGGGAATTACTCAACCTTCTACCCCACTGCCACCAGTTGCCACACCTGTTCCAACTGCCCCGACAGTCTCTCTGATACAAATTCCCACTTCAGGTGGATTCCTTGCACCCAGAGAAGATGTACTAACCAGAGCAGCTGCACTTGGACGTGGTGCAGCCACAACAGGGTTCAAGAGGTTTCTAGCTTTAACAGAAGCTGCAAAGGACCGGAAAGATGGGCCTTTCAGGAAGCTCTTCAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C

Protein Analysis

821

Amino Acids

91.77

Weight (kDa)

6.51

Isoelectric Point (pI)

38.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps53_N	PF04100	3 - 416	5.4e-143	Vps53, N-terminal
VPS53_C	PF16854	646 - 724	9.5e-14	Vacuolar protein sorting-associated protein 53 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006592)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03415 AT4G03415 AT4G03415
fragaria_vesca	FvH4_6g45040 FvH4_6g45040 FvH4_6g45040 FvH4_6g45040
malus_domestica	MD17G1079800.v1.1
prunus_persica	Prupe.3G235000_v2.0.a1
pyrus_communis	pycom09g01530 pycom17g07860
rosa_chinensis	RchiOBHm_Chr2g0162371
rosa_laevigata	RLG00000021340
rosa_multiflora	Rmu_sc0004020.1_g000009
rosa_roxburghii	Rroxscaffold_2G00087980
rosa_rugosa	Rorug02G0496900 Rorug02G0497000 Rorug02G0497100 Rorug02G0497200 Rorug02G0497300 Rorug02G0497400 Rorug02G0497400
rosa_samantha	Rh2AG563100 Rh2CG546100 Rh2DG586000
rosa_wichuraiana	Rw2G046610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1313, 1424, 1652
AatII	GACGTC	1 cut(s)	2006
AccB7I	CCANNNNNTGG	2 cut(s)	899, 1991
AccI	GTMKAC	2 cut(s)	1804, 2194
AccII	CGCG	2 cut(s)	108, 190
AciI	CCGC	6 cut(s)	74, 183, 407, 760, 1440, 1535
AclWI	GGATC	4 cut(s)	180, 211, 758, 1783
AcuI	CTGAAG	3 cut(s)	1026, 2076, 2295
AcvI	CACGTG	2 cut(s)	529, 2009
AcyI	GRCGYC	2 cut(s)	66, 2003
AfaI	GTAC	6 cut(s)	26, 556, 973, 1444, 1738, 2343
AfeI	AGCGCT	1 cut(s)	16
AfiI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2312, 2385
AflIII	ACRYGT	3 cut(s)	526, 528, 1551
AjiI	CACGTC	2 cut(s)	1554, 2369
AjnI	CCWGG	1 cut(s)	1126
Alw21I	GWGCWC	3 cut(s)	486, 1933, 2163
Alw26I	GTCTC	5 cut(s)	245, 358, 1241, 1916, 2293
AlwI	GGATC	4 cut(s)	180, 211, 758, 1783
AlwNI	CAGNNNCTG	3 cut(s)	1466, 1925, 2417
Ama87I	CYCGRG	2 cut(s)	20, 975
Aor51HI	AGCGCT	1 cut(s)	16
AoxI	GGCC	3 cut(s)	1681, 2219, 2438
ArsI	GACNNNNNNTTYG	2 cut(s)	1957, 1989
Asp700I	GAANNNNTTC	3 cut(s)	369, 2061, 2453
AspLEI	GCGC	3 cut(s)	17, 108, 759
AspS9I	GGNCC	5 cut(s)	156, 1877, 2219, 2425, 2438
AsuC2I	CCSGG	2 cut(s)	221, 2223
AsuHPI	GGTGA	5 cut(s)	1522, 1571, 1766, 1821, 1988
AsuII	TTCGAA	1 cut(s)	1051
AvaI	CYCGRG	2 cut(s)	20, 975
AvaII	GGWCC	3 cut(s)	156, 1877, 2425
BanII	GRGCYC	3 cut(s)	486, 1933, 2163
BauI	CACGAG	1 cut(s)	1722
BbrPI	CACGTG	2 cut(s)	529, 2009
BbsI	GAAGAC	3 cut(s)	1214, 1970, 2208
Bbv12I	GWGCWC	3 cut(s)	486, 1933, 2163
BccI	CCATC	7 cut(s)	373, 907, 1089, 1469, 1601, 1993, 2429
BceAI	ACGGC	3 cut(s)	116, 179, 2022
BciT130I	CCWGG	1 cut(s)	1128
BciVI	GTATCC	1 cut(s)	1942
BcnI	CCSGG	2 cut(s)	221, 2223
BcoDI	GTCTC	5 cut(s)	245, 358, 1241, 1916, 2293
BfaI	CTAG	6 cut(s)	713, 860, 1199, 1685, 1797, 2402
BfmI	CTRYAG	2 cut(s)	457, 920
BfoI	RGCGCY	2 cut(s)	18, 760
BfuI	GTATCC	1 cut(s)	1942
BlpI	GCTNAGC	1 cut(s)	240
Bme1390I	CCNGG	3 cut(s)	221, 1128, 2223
Bme18I	GGWCC	3 cut(s)	156, 1877, 2425
BmeT110I	CYCGRG	2 cut(s)	20, 975
BmgBI	CACGTC	2 cut(s)	1554, 2369
BmgT120I	GGNCC	5 cut(s)	156, 1877, 2219, 2425, 2438
BmiI	GGNNCC	1 cut(s)	157
BmrFI	CCNGG	3 cut(s)	221, 1128, 2223
BmsI	GCATC	6 cut(s)	62, 103, 610, 641, 1874, 1929
BpiI	GAAGAC	3 cut(s)	1214, 1970, 2208
BpmI	CTGGAG	3 cut(s)	572, 1149, 1947
Bpu10I	CCTNAGC	2 cut(s)	485, 550
Bpu1102I	GCTNAGC	1 cut(s)	240
Bpu14I	TTCGAA	1 cut(s)	1051
BpuEI	CTTGAG	4 cut(s)	529, 1938, 2147, 2177
BpuMI	CCSGG	2 cut(s)	221, 2223
BsaAI	YACGTR	2 cut(s)	529, 2009
BsaBI	GATNNNNATC	1 cut(s)	2106
BsaHI	GRCGYC	2 cut(s)	66, 2003
BsaJI	CCNNGG	5 cut(s)	165, 195, 1147, 1671, 1730
BsaWI	WCCGGW	1 cut(s)	2427
Bsc4I	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2312, 2385
Bse1I	ACTGG	4 cut(s)	562, 1471, 1930, 2255
Bse3DI	GCAATG	3 cut(s)	905, 1722, 2114
Bse8I	GATNNNNATC	1 cut(s)	2106
BseBI	CCWGG	1 cut(s)	1128
BseDI	CCNNGG	5 cut(s)	165, 195, 1147, 1671, 1730
BseGI	GGATG	5 cut(s)	469, 949, 1261, 1480, 1639
BseJI	GATNNNNATC	1 cut(s)	2106
BseLI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2312, 2385
BseMI	GCAATG	3 cut(s)	905, 1722, 2114
BseMII	CTCAG	4 cut(s)	231, 499, 591, 1560
BseNI	ACTGG	4 cut(s)	562, 1471, 1930, 2255
BseRI	GAGGAG	4 cut(s)	1032, 1259, 1277, 1336
BseYI	CCCAGC	1 cut(s)	1133
BsgI	GTGCAG	5 cut(s)	396, 1357, 1649, 2343, 2394
Bsh1236I	CGCG	2 cut(s)	108, 190
BshFI	GGCC	3 cut(s)	1683, 2221, 2440
BsiHKAI	GWGCWC	3 cut(s)	486, 1933, 2163
BsiHKCI	CYCGRG	2 cut(s)	20, 975
BsiSI	CCGG	4 cut(s)	63, 220, 2222, 2428
BslFI	GGGAC	3 cut(s)	169, 916, 2065
BslI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2312, 2385
BsmAI	GTCTC	5 cut(s)	245, 358, 1241, 1916, 2293
BsmBI	CGTCTC	1 cut(s)	358
BsmFI	GGGAC	3 cut(s)	169, 916, 2065
BsmI	GAATGC	1 cut(s)	828
BsnI	GGCC	3 cut(s)	1683, 2221, 2440
BsoBI	CYCGRG	2 cut(s)	20, 975
Bsp119I	TTCGAA	1 cut(s)	1051
Bsp1286I	GDGCHC	3 cut(s)	486, 1933, 2163
Bsp143I	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
Bsp1720I	GCTNAGC	1 cut(s)	240
Bsp19I	CCATGG	1 cut(s)	1730
BspACI	CCGC	6 cut(s)	74, 183, 407, 760, 1440, 1535
BspANI	GGCC	3 cut(s)	1683, 2221, 2440
BspCNI	CTCAG	4 cut(s)	232, 498, 592, 1561
BspFNI	CGCG	2 cut(s)	108, 190
BspLI	GGNNCC	1 cut(s)	157
BspPI	GGATC	4 cut(s)	180, 211, 758, 1783
BspQI	GCTCTTC	2 cut(s)	336, 2459
BspT104I	TTCGAA	1 cut(s)	1051
BsrDI	GCAATG	3 cut(s)	905, 1722, 2114
BsrI	ACTGG	4 cut(s)	562, 1471, 1930, 2255
BssECI	CCNNGG	5 cut(s)	165, 195, 1147, 1671, 1730
BssMI	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
BssNAI	GTATAC	2 cut(s)	1805, 2195
BssNI	GRCGYC	2 cut(s)	66, 2003
BssSI	CACGAG	1 cut(s)	1722
BssT1I	CCWWGG	3 cut(s)	165, 1671, 1730
Bst1107I	GTATAC	2 cut(s)	1805, 2195
Bst2BI	CACGAG	1 cut(s)	1722
Bst2UI	CCWGG	1 cut(s)	1128
Bst4CI	ACNGT	6 cut(s)	95, 417, 1186, 1335, 2079, 2287
Bst6I	CTCTTC	3 cut(s)	336, 1197, 2459
BstACI	GRCGYC	2 cut(s)	66, 2003
BstAPI	GCANNNNNTGC	1 cut(s)	2021
BstBAI	YACGTR	2 cut(s)	529, 2009
BstBI	TTCGAA	1 cut(s)	1051
BstC8I	GCNNGC	6 cut(s)	192, 607, 1074, 1298, 1910, 2017
BstDEI	CTNAG	8 cut(s)	240, 300, 485, 550, 600, 610, 717, 1569
BstDSI	CCRYGG	3 cut(s)	195, 1147, 1730
BstEII	GGTNACC	3 cut(s)	1666, 1754, 1994
BstF5I	GGATG	5 cut(s)	469, 949, 1261, 1480, 1639
BstFNI	CGCG	2 cut(s)	108, 190
BstH2I	RGCGCY	2 cut(s)	18, 760
BstHHI	GCGC	3 cut(s)	17, 108, 759
BstKTI	GATC	6 cut(s)	175, 219, 753, 1791, 1836, 2185
BstMAI	GTCTC	5 cut(s)	245, 358, 1241, 1916, 2293
BstMBI	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
BstMWI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2021
BstNI	CCWGG	1 cut(s)	1128
BstNSI	RCATGY	2 cut(s)	812, 1300
BstPI	GGTNACC	3 cut(s)	1666, 1754, 1994
BstSCI	CCNGG	3 cut(s)	219, 1126, 2221
BstSFI	CTRYAG	2 cut(s)	457, 920
BstUI	CGCG	2 cut(s)	108, 190
BstV2I	GAAGAC	3 cut(s)	1214, 1970, 2208
BstX2I	RGATCY	2 cut(s)	172, 216
BstYI	RGATCY	2 cut(s)	172, 216
BstZ17I	GTATAC	2 cut(s)	1805, 2195
BsuI	GTATCC	1 cut(s)	1942
BsuRI	GGCC	3 cut(s)	1683, 2221, 2440
BtgI	CCRYGG	3 cut(s)	195, 1147, 1730
BtrI	CACGTC	2 cut(s)	1554, 2369
BtsCI	GGATG	5 cut(s)	469, 949, 1261, 1480, 1639
BtsI	GCAGTG	2 cut(s)	1345, 2246
BtsIMutI	CAGTG	3 cut(s)	100, 1345, 2246
Cac8I	GCNNGC	6 cut(s)	192, 607, 1074, 1298, 1910, 2017
CaiI	CAGNNNCTG	3 cut(s)	1466, 1925, 2417
CfoI	GCGC	3 cut(s)	17, 108, 759
Cfr13I	GGNCC	5 cut(s)	156, 1877, 2219, 2425, 2438
CseI	GACGC	1 cut(s)	55
Csp6I	GTAC	6 cut(s)	25, 555, 972, 1443, 1737, 2342
CspCI	CAANNNNNGTGG	2 cut(s)	1129, 1164
CviQI	GTAC	6 cut(s)	25, 555, 972, 1443, 1737, 2342
DdeI	CTNAG	8 cut(s)	240, 300, 485, 550, 600, 610, 717, 1569
DpnI	GATC	6 cut(s)	174, 218, 752, 1790, 1835, 2184
DpnII	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
Eam1104I	CTCTTC	3 cut(s)	336, 1197, 2459
EarI	CTCTTC	3 cut(s)	336, 1197, 2459
Ecl136II	GAGCTC	3 cut(s)	484, 1931, 2161
Eco130I	CCWWGG	3 cut(s)	165, 1671, 1730
Eco24I	GRGCYC	3 cut(s)	486, 1933, 2163
Eco32I	GATATC	2 cut(s)	868, 1981
Eco47I	GGWCC	3 cut(s)	156, 1877, 2425
Eco47III	AGCGCT	1 cut(s)	16
Eco53kI	GAGCTC	3 cut(s)	484, 1931, 2161
Eco57I	CTGAAG	3 cut(s)	1026, 2076, 2295
Eco72I	CACGTG	2 cut(s)	529, 2009
Eco88I	CYCGRG	2 cut(s)	20, 975
Eco91I	GGTNACC	3 cut(s)	1666, 1754, 1994
EcoICRI	GAGCTC	3 cut(s)	484, 1931, 2161
EcoO65I	GGTNACC	3 cut(s)	1666, 1754, 1994
EcoRI	GAATTC	1 cut(s)	2151
EcoRII	CCWGG	1 cut(s)	1126
EcoRV	GATATC	2 cut(s)	868, 1981
EcoT14I	CCWWGG	3 cut(s)	165, 1671, 1730
EcoT22I	ATGCAT	2 cut(s)	625, 748
EcoT38I	GRGCYC	3 cut(s)	486, 1933, 2163
ErhI	CCWWGG	3 cut(s)	165, 1671, 1730
Esp3I	CGTCTC	1 cut(s)	358
FalI	AAGNNNNNCTT	4 cut(s)	855, 887, 1310, 1342
FaqI	GGGAC	3 cut(s)	169, 916, 2065
FauNDI	CATATG	1 cut(s)	1405
FblI	GTMKAC	2 cut(s)	1804, 2194
FokI	GGATG	5 cut(s)	476, 956, 1268, 1487, 1646
FriOI	GRGCYC	3 cut(s)	486, 1933, 2163
FspBI	CTAG	6 cut(s)	713, 860, 1199, 1685, 1797, 2402
GlaI	GCGC	3 cut(s)	16, 107, 758
GsaI	CCCAGC	1 cut(s)	1137
GsuI	CTGGAG	3 cut(s)	572, 1149, 1947
HaeII	RGCGCY	2 cut(s)	18, 760
HaeIII	GGCC	3 cut(s)	1683, 2221, 2440
HapII	CCGG	4 cut(s)	63, 220, 2222, 2428
HgaI	GACGC	1 cut(s)	55
HhaI	GCGC	3 cut(s)	17, 108, 759
Hin1I	GRCGYC	2 cut(s)	66, 2003
Hin6I	GCGC	3 cut(s)	15, 106, 757
HinP1I	GCGC	3 cut(s)	15, 106, 757
HincII	GTYRAC	1 cut(s)	140
HindII	GTYRAC	1 cut(s)	140
HindIII	AAGCTT	3 cut(s)	547, 1070, 1415
HpaII	CCGG	4 cut(s)	63, 220, 2222, 2428
HphI	GGTGA	5 cut(s)	1522, 1571, 1766, 1821, 1988
Hpy166II	GTNNAC	8 cut(s)	140, 430, 1189, 1562, 1739, 1805, 1880, 2195
Hpy8I	GTNNAC	8 cut(s)	140, 430, 1189, 1562, 1739, 1805, 1880, 2195
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	4 cut(s)	695, 807, 1832, 2247
HpyCH4III	ACNGT	6 cut(s)	95, 417, 1186, 1335, 2079, 2287
HpyCH4IV	ACGT	6 cut(s)	528, 1553, 1882, 2003, 2008, 2368
HpyF10VI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2021
HpyF3I	CTNAG	8 cut(s)	240, 300, 485, 550, 600, 610, 717, 1569
HpySE526I	ACGT	6 cut(s)	528, 1553, 1882, 2003, 2008, 2368
Hsp92I	GRCGYC	2 cut(s)	66, 2003
HspAI	GCGC	3 cut(s)	15, 106, 757
Kzo9I	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
LguI	GCTCTTC	2 cut(s)	336, 2459
LmnI	GCTCC	5 cut(s)	673, 704, 1130, 1323, 1928
LweI	GCATC	6 cut(s)	62, 103, 610, 641, 1874, 1929
MaeI	CTAG	6 cut(s)	713, 860, 1199, 1685, 1797, 2402
MaeII	ACGT	6 cut(s)	528, 1553, 1882, 2003, 2008, 2368
MaeIII	GTNAC	7 cut(s)	275, 522, 1517, 1666, 1754, 1994, 2004
MalI	GATC	6 cut(s)	174, 218, 752, 1790, 1835, 2184
MboI	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
MfeI	CAATTG	1 cut(s)	2085
MflI	RGATCY	2 cut(s)	172, 216
MhlI	GDGCHC	3 cut(s)	486, 1933, 2163
MlyI	GAGTC	2 cut(s)	575, 816
MmeI	TCCRAC	7 cut(s)	270, 399, 549, 702, 970, 1602, 2297
Mph1103I	ATGCAT	2 cut(s)	625, 748
MroXI	GAANNNNTTC	3 cut(s)	369, 2061, 2453
MslI	CAYNNNNRTG	1 cut(s)	1784
MspA1I	CMGCKG	2 cut(s)	1535, 2357
MspI	CCGG	4 cut(s)	63, 220, 2222, 2428
MspR9I	CCNGG	3 cut(s)	221, 1128, 2223
MunI	CAATTG	1 cut(s)	2085
Mva1269I	GAATGC	1 cut(s)	828
MvaI	CCWGG	1 cut(s)	1128
MvnI	CGCG	2 cut(s)	108, 190
MwoI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2021
NciI	CCSGG	2 cut(s)	221, 2223
NcoI	CCATGG	1 cut(s)	1730
NdeI	CATATG	1 cut(s)	1405
NdeII	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
NlaIV	GGNNCC	1 cut(s)	157
NmuCI	GTSAC	5 cut(s)	275, 522, 1754, 1994, 2004
NsiI	ATGCAT	2 cut(s)	625, 748
NspI	RCATGY	2 cut(s)	812, 1300
NspV	TTCGAA	1 cut(s)	1051
PaeI	GCATGC	1 cut(s)	1300
PaeR7I	CTCGAG	2 cut(s)	20, 975
PciSI	GCTCTTC	2 cut(s)	336, 2459
PcsI	WCGNNNNNNNCGW	1 cut(s)	534
PctI	GAATGC	1 cut(s)	828
PdmI	GAANNNNTTC	3 cut(s)	369, 2061, 2453
PfeI	GAWTC	8 cut(s)	88, 575, 1152, 1354, 1393, 1592, 2090, 2319
PflMI	CCANNNNNTGG	2 cut(s)	899, 1991
PfoI	TCCNGGA	1 cut(s)	219
PleI	GAGTC	2 cut(s)	574, 816
PmaCI	CACGTG	2 cut(s)	529, 2009
PmlI	CACGTG	2 cut(s)	529, 2009
PpsI	GAGTC	2 cut(s)	574, 816
Ppu21I	YACGTR	2 cut(s)	529, 2009
PsiI	TTATAA	3 cut(s)	1313, 1424, 1652
Psp124BI	GAGCTC	3 cut(s)	486, 1933, 2163
Psp6I	CCWGG	1 cut(s)	1126
PspCI	CACGTG	2 cut(s)	529, 2009
PspEI	GGTNACC	3 cut(s)	1666, 1754, 1994
PspFI	CCCAGC	1 cut(s)	1133
PspGI	CCWGG	1 cut(s)	1126
PspN4I	GGNNCC	1 cut(s)	157
PspPI	GGNCC	5 cut(s)	156, 1877, 2219, 2425, 2438
PspXI	VCTCGAGB	1 cut(s)	975
PstNI	CAGNNNCTG	3 cut(s)	1466, 1925, 2417
PsuI	RGATCY	2 cut(s)	172, 216
PvuII	CAGCTG	1 cut(s)	2357
RsaI	GTAC	6 cut(s)	26, 556, 973, 1444, 1738, 2343
RsaNI	GTAC	6 cut(s)	25, 555, 972, 1443, 1737, 2342
RseI	CAYNNNNRTG	1 cut(s)	1784
SacI	GAGCTC	3 cut(s)	486, 1933, 2163
SapI	GCTCTTC	2 cut(s)	336, 2459
Sau3AI	GATC	6 cut(s)	172, 216, 750, 1788, 1833, 2182
Sau96I	GGNCC	5 cut(s)	156, 1877, 2219, 2425, 2438
SchI	GAGTC	2 cut(s)	575, 816
ScrFI	CCNGG	3 cut(s)	221, 1128, 2223
SduI	GDGCHC	3 cut(s)	486, 1933, 2163
SfaNI	GCATC	6 cut(s)	62, 103, 610, 641, 1874, 1929
SfcI	CTRYAG	2 cut(s)	457, 920
Sfr274I	CTCGAG	2 cut(s)	20, 975
SfuI	TTCGAA	1 cut(s)	1051
SinI	GGWCC	3 cut(s)	156, 1877, 2425
SlaI	CTCGAG	2 cut(s)	20, 975
SmiMI	CAYNNNNRTG	1 cut(s)	1784
SmlI	CTYRAG	6 cut(s)	20, 544, 975, 1953, 2156, 2162
SmoI	CTYRAG	6 cut(s)	20, 544, 975, 1953, 2156, 2162
SphI	GCATGC	1 cut(s)	1300
SsiI	CCGC	6 cut(s)	74, 183, 407, 760, 1440, 1535
SspI	AATATT	3 cut(s)	516, 696, 1541
SspMI	CTAG	6 cut(s)	713, 860, 1199, 1685, 1797, 2402
SstI	GAGCTC	3 cut(s)	486, 1933, 2163
StyD4I	CCNGG	3 cut(s)	219, 1126, 2221
StyI	CCWWGG	3 cut(s)	165, 1671, 1730
TaaI	ACNGT	6 cut(s)	95, 417, 1186, 1335, 2079, 2287
TaiI	ACGT	6 cut(s)	531, 1556, 1885, 2006, 2011, 2371
TaqI	TCGA	5 cut(s)	21, 69, 976, 1051, 1170
TatI	WGTACW	2 cut(s)	24, 2341
TauI	GCSGC	4 cut(s)	76, 185, 410, 762
TfiI	GAWTC	8 cut(s)	88, 575, 1152, 1354, 1393, 1592, 2090, 2319
TscAI	CASTG	3 cut(s)	100, 1345, 2253
TseFI	GTSAC	5 cut(s)	275, 522, 1754, 1994, 2004
Tsp45I	GTSAC	5 cut(s)	275, 522, 1754, 1994, 2004
TspDTI	ATGAA	7 cut(s)	323, 789, 792, 960, 1077, 1650, 1719
TspGWI	ACGGA	1 cut(s)	1136
TspRI	CASTG	3 cut(s)	100, 1345, 2253
Van91I	CCANNNNNTGG	2 cut(s)	899, 1991
VpaK11BI	GGWCC	3 cut(s)	156, 1877, 2425
XceI	RCATGY	2 cut(s)	812, 1300
XhoI	CTCGAG	2 cut(s)	20, 975
XmiI	GTMKAC	2 cut(s)	1804, 2194
XmnI	GAANNNNTTC	3 cut(s)	369, 2061, 2453
XspI	CTAG	6 cut(s)	713, 860, 1199, 1685, 1797, 2402
ZraI	GACGTC	1 cut(s)	2004
Zsp2I	ATGCAT	2 cut(s)	625, 748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.