pycom17g11990
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007799
Physical Location & Seq
Reverse (-)
9775567 .. 9780209
4643 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGATCGATCGGGACTTCAGGAGCACGATAACTCGTGAAAAGTTTGAAGAGCTCTGCGAAGATCTGTGGGAGAAGTCGCTAATACCTCTTAAGGAAGTGCTCAAGCATTCTGGTTTAAAGGTAGATGAGATATATGCAGTGGAACTGATAGGAGGAGCTACTAGGGTGCCAAAGTTGCAGGCTAAGCTTCAGGAATATCTTGGAAGGAAAGAGTTGGATAGACATCTGGATGCTGATGAAGCTACAGTTCTTGGTGCAGCATTATATGCTGCTAATTTAAGTGATGGAATCAAACTGAACCGTAAGTTAGGGATGATTGATGGTTCTACCTATGGGTTTGTGCTTGAGTTAGATGGCCCCGATCTTCAGAAAGAAGATAGCACTAGGCAGACACTTGTGCAACGGATGAAGAAACTCCCCAGCAAGATGTTCAGGTCCTTTATCCAGAGCAAAGATTTTGAAGTGACACTAGCATACGAGAGTGAAGACCTTTTACCCCCTGGTGCCACCTCTCCTGTATTTGGTCAGTACTCTGTGTCCAGTTTGACAGAGACAAGTGAGAAGTATGCATCACGAAATTTGTCTTCACCCATTAAAGCAAGTCTGCATTTCTCTTTGAGTAGAAGTGGTGTTTTGTCGTTCGATCGTGCAGATGCTTTTATTGAAGTGTCAGAATGGGTAGAAGTTCCTAAGAAGAATCTGACTACTCAGGAGAATTCAACTAATGTTGCACCCAACATATCAACTGAAACTGGTGCTCAGAACACATCAAAAGAAAGCAATGGCAATACTGATGATGGTGGAAATAGTAACAGTTCCAACTCTACAGTAGAAACAGACATCGTTATTGAAAAAAAGCTGAAAAAGCGGACCTTTAGGATTCCACTGAAGGTGCAAGAGTGGCTTTACACCGATGGTGAAGATGCTACTGCTTCAGAGTTTCAGGAACGCCTAGATGTGCTAAAAGCTATTGGTGATCCAATATTCTTCAGATTCAAAGAGCTCACTGCTCGGCCAGAAGCAGTGGAACACGCTCGAAAGTACCTTGTTGAAGTGCAACAGGTTGCGAGTGATGCTGACAAGTTGAAGAAGTGGTTGGACGAGAAGGAGGATGCGCAGAAAAAGACTCCCGCACATAGCAAACCGGCATTCACATCGGATGAAGTGTTCGGGAAGGTTGCTAGAGTCAACAGAATCCCCAAGCCAAAGCCTAAAATTGAGAAACCCACAAGCAATGAAACCGAGAGCAGCGGAGAAAAAGCTAAGGATTCTGACTCGAGTTCTGATAATTCTTCACAAGATGACCAGAAAGCCGGAGACTCAGATGACTCAGCAAATGAAAAAGTTGAGTCTGAGGGTCACGATGAGCTGTGA

Protein Analysis

458

Amino Acids

50.96

Weight (kDa)

5.08

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 3 - 329 5.5e-29 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1116
AccB1I GGYRCC 2 cut(s) 166, 503
AciI CCGC 3 cut(s) 868, 1131, 1251
AclWI GGATC 1 cut(s) 971
AcoI YGGCCR 1 cut(s) 1013
AcsI RAATTY 2 cut(s) 577, 715
AcuI CTGAAG 5 cut(s) 173, 350, 908, 918, 973
AfaI GTAC 2 cut(s) 530, 1043
AfiI CCNNNNNNNGG 1 cut(s) 521
AflII CTTAAG 1 cut(s) 89
AgsI TTSAA 8 cut(s) 47, 461, 665, 720, 851, 997, 1052, 1087
AjnI CCWGG 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 1079, 1111
AloI GAACNNNNNNTCC 2 cut(s) 1151, 1183
AluBI AGCT 9 cut(s) 52, 158, 187, 242, 859, 968, 1003, 1262, 1369
AluI AGCT 9 cut(s) 52, 158, 187, 242, 859, 968, 1003, 1262, 1369
Alw21I GWGCWC 5 cut(s) 26, 54, 102, 760, 1005
Alw26I GTCTC 2 cut(s) 545, 1311
AlwI GGATC 1 cut(s) 971
Ama87I CYCGRG 1 cut(s) 1276
AoxI GGCC 2 cut(s) 355, 1013
ApeKI GCWGC 3 cut(s) 257, 269, 1248
ApoI RAATTY 2 cut(s) 577, 715
AspLEI GCGC 1 cut(s) 1117
AspS9I GGNCC 3 cut(s) 356, 435, 870
AsuHPI GGTGA 3 cut(s) 579, 929, 986
AvaI CYCGRG 1 cut(s) 1276
AvaII GGWCC 2 cut(s) 435, 870
BanI GGYRCC 2 cut(s) 166, 503
BanII GRGCYC 2 cut(s) 54, 1005
BarI GAAGNNNNNNTAC 2 cut(s) 477, 509
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 492, 576
Bbv12I GWGCWC 5 cut(s) 26, 54, 102, 760, 1005
BbvI GCAGC 3 cut(s) 256, 269, 1260
BccI CCATC 5 cut(s) 278, 314, 347, 791, 908
BcgI CGANNNNNNTGC 2 cut(s) 1137, 1171
BciT130I CCWGG 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 545, 1311
BfaI CTAG 5 cut(s) 162, 384, 470, 953, 1182
BfmI CTRYAG 2 cut(s) 243, 825
BfrI CTTAAG 1 cut(s) 89
BglII AGATCT 1 cut(s) 61
BisI GCNGC 3 cut(s) 258, 270, 1249
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 3 cut(s) 259, 271, 1250
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 1 cut(s) 501
Bme18I GGWCC 2 cut(s) 435, 870
BmeT110I CYCGRG 1 cut(s) 1276
BmgT120I GGNCC 3 cut(s) 356, 435, 870
BmiI GGNNCC 3 cut(s) 168, 358, 505
BmrFI CCNGG 1 cut(s) 501
BmsI GCATC 6 cut(s) 220, 578, 643, 913, 1063, 1102
BpiI GAAGAC 2 cut(s) 492, 576
Bpu10I CCTNAGC 1 cut(s) 1263
Bpu1102I GCTNAGC 1 cut(s) 183
BpuEI CTTGAG 2 cut(s) 86, 365
Bsa29I ATCGAT 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 499
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse118I RCCGGY 1 cut(s) 1144
Bse1I ACTGG 2 cut(s) 540, 757
Bse3DI GCAATG 2 cut(s) 787, 1240
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 1 cut(s) 501
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 499
BseGI GGATG 5 cut(s) 235, 318, 411, 1117, 1165
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMI GCAATG 2 cut(s) 787, 1240
BseMII CTCAG 5 cut(s) 722, 773, 1335, 1344, 1344
BseNI ACTGG 2 cut(s) 540, 757
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 3 cut(s) 256, 269, 1260
BseYI CCCAGC 1 cut(s) 419
BsgI GTGCAG 2 cut(s) 276, 669
Bsh1285I CGRYCG 2 cut(s) 10, 646
BshFI GGCC 2 cut(s) 357, 1015
BshNI GGYRCC 2 cut(s) 166, 503
BshVI ATCGAT 1 cut(s) 6
BsiEI CGRYCG 2 cut(s) 10, 646
BsiHKAI GWGCWC 5 cut(s) 26, 54, 102, 760, 1005
BsiHKCI CYCGRG 1 cut(s) 1276
BsiSI CCGG 2 cut(s) 1145, 1314
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 2 cut(s) 545, 1311
BsmFI GGGAC 1 cut(s) 26
BsmI GAATGC 2 cut(s) 106, 1148
BsnI GGCC 2 cut(s) 357, 1015
BsoBI CYCGRG 1 cut(s) 1276
Bsp1286I GDGCHC 5 cut(s) 26, 54, 102, 760, 1005
Bsp143I GATC 6 cut(s) 3, 7, 61, 361, 643, 976
Bsp1720I GCTNAGC 1 cut(s) 183
BspACI CCGC 3 cut(s) 868, 1131, 1251
BspANI GGCC 2 cut(s) 357, 1015
BspCNI CTCAG 5 cut(s) 721, 772, 1334, 1343, 1345
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 3 cut(s) 168, 358, 505
BspPI GGATC 1 cut(s) 971
BspQI GCTCTTC 1 cut(s) 42
BspT107I GGYRCC 2 cut(s) 166, 503
BspTI CTTAAG 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 787, 1240
BsrFI RCCGGY 1 cut(s) 1144
BsrI ACTGG 2 cut(s) 540, 757
BssAI RCCGGY 1 cut(s) 1144
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 6 cut(s) 3, 7, 61, 361, 643, 976
BssSI CACGAG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 4 cut(s) 247, 302, 815, 829
Bst6I CTCTTC 1 cut(s) 42
BstAFI CTTAAG 1 cut(s) 89
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 8 cut(s) 183, 690, 708, 759, 1263, 1321, 1330, 1353
BstF5I GGATG 5 cut(s) 235, 318, 411, 1117, 1165
BstHHI GCGC 1 cut(s) 1117
BstKTI GATC 6 cut(s) 6, 10, 64, 364, 646, 979
BstMAI GTCTC 2 cut(s) 545, 1311
BstMBI GATC 6 cut(s) 3, 7, 61, 361, 643, 976
BstMCI CGRYCG 2 cut(s) 10, 646
BstMWI GCNNNNNNNGC 7 cut(s) 175, 184, 239, 266, 865, 901, 1073
BstNI CCWGG 1 cut(s) 501
BstSCI CCNGG 1 cut(s) 499
BstSFI CTRYAG 2 cut(s) 243, 825
BstV1I GCAGC 3 cut(s) 256, 269, 1260
BstV2I GAAGAC 2 cut(s) 492, 576
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 2 cut(s) 357, 1015
BsuTUI ATCGAT 1 cut(s) 6
BtsCI GGATG 5 cut(s) 235, 318, 411, 1117, 1165
BtsI GCAGTG 3 cut(s) 144, 1005, 1029
BtsIMutI CAGTG 4 cut(s) 144, 884, 1005, 1029
Cac8I GCNNGC 1 cut(s) 180
CfoI GCGC 1 cut(s) 1117
Cfr10I RCCGGY 1 cut(s) 1144
Cfr13I GGNCC 3 cut(s) 356, 435, 870
ClaI ATCGAT 1 cut(s) 6
Csp6I GTAC 2 cut(s) 529, 1042
CviQI GTAC 2 cut(s) 529, 1042
DdeI CTNAG 8 cut(s) 183, 690, 708, 759, 1263, 1321, 1330, 1353
DpnI GATC 6 cut(s) 5, 9, 63, 363, 645, 978
DpnII GATC 6 cut(s) 3, 7, 61, 361, 643, 976
DraI TTTAAA 1 cut(s) 117
EaeI YGGCCR 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 2 cut(s) 52, 1003
Eco24I GRGCYC 2 cut(s) 54, 1005
Eco47I GGWCC 2 cut(s) 435, 870
Eco53kI GAGCTC 2 cut(s) 52, 1003
Eco57I CTGAAG 5 cut(s) 173, 350, 908, 918, 973
Eco88I CYCGRG 1 cut(s) 1276
EcoICRI GAGCTC 2 cut(s) 52, 1003
EcoO109I RGGNCCY 1 cut(s) 435
EcoRI GAATTC 1 cut(s) 715
EcoRII CCWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 571
EcoT38I GRGCYC 2 cut(s) 54, 1005
FaiI YATR 9 cut(s) 133, 135, 265, 267, 333, 475, 567, 740, 1137
FalI AAGNNNNNCTT 4 cut(s) 857, 889, 888, 920
FaqI GGGAC 1 cut(s) 26
FauI CCCGC 1 cut(s) 1138
Fnu4HI GCNGC 3 cut(s) 258, 270, 1249
FokI GGATG 5 cut(s) 242, 325, 418, 1124, 1172
FriOI GRGCYC 2 cut(s) 54, 1005
Fsp4HI GCNGC 3 cut(s) 258, 270, 1249
FspBI CTAG 5 cut(s) 162, 384, 470, 953, 1182
FspI TGCGCA 1 cut(s) 1116
GlaI GCGC 1 cut(s) 1116
GluI GCNGC 3 cut(s) 258, 270, 1249
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 2 cut(s) 357, 1015
HapII CCGG 2 cut(s) 1145, 1314
HhaI GCGC 1 cut(s) 1117
Hin6I GCGC 1 cut(s) 1115
HinP1I GCGC 1 cut(s) 1115
HincII GTYRAC 1 cut(s) 1189
HindII GTYRAC 1 cut(s) 1189
HindIII AAGCTT 1 cut(s) 185
HpaII CCGG 2 cut(s) 1145, 1314
HphI GGTGA 3 cut(s) 579, 929, 986
Hpy166II GTNNAC 1 cut(s) 1189
Hpy8I GTNNAC 1 cut(s) 1189
HpyAV CCTTC 4 cut(s) 198, 883, 1099, 1168
HpyCH4III ACNGT 4 cut(s) 247, 302, 815, 829
HpyF10VI GCNNNNNNNGC 7 cut(s) 175, 184, 239, 266, 865, 901, 1073
HpyF3I CTNAG 8 cut(s) 183, 690, 708, 759, 1263, 1321, 1330, 1353
HspAI GCGC 1 cut(s) 1115
Kzo9I GATC 6 cut(s) 3, 7, 61, 361, 643, 976
LguI GCTCTTC 1 cut(s) 42
LmnI GCTCC 2 cut(s) 21, 155
Lsp1109I GCAGC 3 cut(s) 256, 269, 1260
LweI GCATC 6 cut(s) 220, 578, 643, 913, 1063, 1102
MaeI CTAG 5 cut(s) 162, 384, 470, 953, 1182
MaeIII GTNAC 3 cut(s) 463, 809, 1358
MalI GATC 6 cut(s) 5, 9, 63, 363, 645, 978
MboI GATC 6 cut(s) 3, 7, 61, 361, 643, 976
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 5 cut(s) 26, 54, 102, 760, 1005
MluCI AATT 5 cut(s) 274, 577, 715, 1215, 1288
MlyI GAGTC 6 cut(s) 1120, 1194, 1268, 1313, 1322, 1358
MmeI TCCRAC 3 cut(s) 195, 843, 1077
MnlI CCTC 5 cut(s) 96, 146, 520, 1102, 1348
Mph1103I ATGCAT 1 cut(s) 571
MseI TTAA 4 cut(s) 90, 116, 278, 594
MslI CAYNNNNRTG 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 1251
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 2 cut(s) 1145, 1314
MspR9I CCNGG 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 106, 1148
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 7 cut(s) 175, 184, 239, 266, 865, 901, 1073
NdeII GATC 6 cut(s) 3, 7, 61, 361, 643, 976
NlaIV GGNNCC 3 cut(s) 168, 358, 505
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 2 cut(s) 463, 1358
NsbI TGCGCA 1 cut(s) 1116
NsiI ATGCAT 1 cut(s) 571
PaeR7I CTCGAG 1 cut(s) 1276
PciSI GCTCTTC 1 cut(s) 42
PctI GAATGC 2 cut(s) 106, 1148
PfeI GAWTC 6 cut(s) 288, 697, 880, 993, 1194, 1268
PkrI GCNGC 3 cut(s) 259, 271, 1250
Ple19I CGATCG 2 cut(s) 10, 646
PleI GAGTC 6 cut(s) 1120, 1193, 1268, 1313, 1322, 1357
PpsI GAGTC 6 cut(s) 1120, 1193, 1268, 1313, 1322, 1357
PpuMI RGGWCCY 1 cut(s) 435
Psp124BI GAGCTC 2 cut(s) 54, 1005
Psp5II RGGWCCY 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 3 cut(s) 168, 358, 505
PspPI GGNCC 3 cut(s) 356, 435, 870
PspPPI RGGWCCY 1 cut(s) 435
PspXI VCTCGAGB 1 cut(s) 1276
PsuI RGATCY 1 cut(s) 61
PvuI CGATCG 2 cut(s) 10, 646
RsaI GTAC 2 cut(s) 530, 1043
RsaNI GTAC 2 cut(s) 529, 1042
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 2 cut(s) 54, 1005
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 4 cut(s) 90, 116, 278, 594
SatI GCNGC 3 cut(s) 258, 270, 1249
Sau3AI GATC 6 cut(s) 3, 7, 61, 361, 643, 976
Sau96I GGNCC 3 cut(s) 356, 435, 870
ScaI AGTACT 1 cut(s) 530
SchI GAGTC 6 cut(s) 1120, 1194, 1268, 1313, 1322, 1358
ScrFI CCNGG 1 cut(s) 501
SduI GDGCHC 5 cut(s) 26, 54, 102, 760, 1005
SfaNI GCATC 6 cut(s) 220, 578, 643, 913, 1063, 1102
SfcI CTRYAG 2 cut(s) 243, 825
Sfr274I CTCGAG 1 cut(s) 1276
SinI GGWCC 2 cut(s) 435, 870
SlaI CTCGAG 1 cut(s) 1276
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 4 cut(s) 89, 101, 344, 1276
SmoI CTYRAG 4 cut(s) 89, 101, 344, 1276
Sse9I AATT 5 cut(s) 274, 577, 715, 1215, 1288
SsiI CCGC 3 cut(s) 868, 1131, 1251
SspI AATATT 1 cut(s) 984
SspMI CTAG 5 cut(s) 162, 384, 470, 953, 1182
SstI GAGCTC 2 cut(s) 54, 1005
StyD4I CCNGG 1 cut(s) 499
TaaI ACNGT 4 cut(s) 247, 302, 815, 829
TaqI TCGA 4 cut(s) 6, 642, 1036, 1277
TasI AATT 5 cut(s) 274, 577, 715, 1215, 1288
TatI WGTACW 1 cut(s) 528
TfiI GAWTC 6 cut(s) 288, 697, 880, 993, 1194, 1268
Tru1I TTAA 4 cut(s) 90, 116, 278, 594
Tru9I TTAA 4 cut(s) 90, 116, 278, 594
TscAI CASTG 4 cut(s) 144, 891, 1012, 1029
TseFI GTSAC 2 cut(s) 463, 1358
TseI GCWGC 3 cut(s) 257, 269, 1248
Tsp45I GTSAC 2 cut(s) 463, 1358
TspDTI ATGAA 5 cut(s) 252, 422, 1176, 1251, 1353
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 4 cut(s) 144, 891, 1012, 1029
Vha464I CTTAAG 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 435, 870
XapI RAATTY 2 cut(s) 577, 715
XhoI CTCGAG 1 cut(s) 1276
XspI CTAG 5 cut(s) 162, 384, 470, 953, 1182
ZrmI AGTACT 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.