pycom17g13170

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008876
Physical Location & Seq
Forward (+)
10848614 .. 10854621
6008 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2409 bp
ATGGGGAGCGAGACGAAGAATGGAGTGGGGGTAGCGGCGGCGCAGTTTCCGCCGACGACGAGGAAGATTATACAGAGCCTGAAGGAGATTGTTAACTGTCCGGAGCCTGAGATCTACTCGGTTCTTAAAGAGTGCAATATGGACCCGAGCGACGCCGTTCAGCGCCTGCTCTCTTTAGATACTTTTCATGAAGTCAGGAGCAAACGTGAAAGAAGGAAAGAGATAAAGGAGACACAGGACACCAAGTCGCGAGTTCACAATGCAGGTTCAAATCGTGGTGCTAGAGGTATTAGCGAGCGTACTGGTGGATGGTGTGGATCAACTCAAACCAGTTCCAATGAGCTTGGTAAAGCAGTGTATAAGGGACAGAATGGGTTTGTTGCTCCTTCACCTCATACTTCAGCTTCTCACTTTACAGGAGGAACCACAAGCCAGCAACCTTCATCTCAGCATGAATCTTTTAGTACTTCCATAAGGCCAGGTGATGCAGTCTCTGCATCTCTACAACCTTCTGCGGGAAATCAATCTACTTATGGAACCAACTTGGGGAATCTTTCAATGGCCGACATTGTTAAGATGCGTAGACAACCTAGCATGGGCTTACGTATTTCATCTGATACATCTTCACATCAGGATGCATTTTCAACATATTCATGTAATAGCCACGTGGAATCTTCCCAAACAAAGATGTACCAATATATGCACTCTCATAATCCTCGTGTGTCAGAGATGTTACATAAAACTGGCGATAGTGCTGGACAGAATGATTTCCATGATGAATGGCATGTGATTGAGCAGCCAACAGCTGCAAATAGGTCATCAATTTTAAATGCAAATCAATCTAAGTTTTATATCAATGAATCCAACGTGCCAAGGGATTACCAGTCACATAAGGTCCAAGAATCAGAGGGGAATATTAGTAGCGAAAGTGTTAGCTCAGATTGCAATGCTTATGCTTTTGCTTCCAGTAGACAGAAGATGGCGGTTACTTCCTGTGGAAAATCATATTGTGTTGATGACTTGTCTTCCAATTCTAGTTCCTACGAATCTCATAGGTCTGCATATGAGAATGGAGAAGCAGCTACTGTGAGAATGCAACAACTGAATCTAAGAAAGGATGAAGATCGAGATAATTGCGCAGTAGTCCTTCCAAATAATTTGCAAGAATTGGCTGCCGATTGTTCGCATTTGAGTTTTGGCACCTTTAGACCGGCTCAAAGTTCTGCATTATCGAGCAAGCCATCTAATTCCTTGAAAAATGACTTGGGAGGATCTTCTGTAGGAGTAGATGTTTTCTCAGCCGCTCGCTTGGATACAAGAAATTCAGGGTATAAGCACGACGAATTTCGTGGATCCTTATATGATACTAGAAGAGCAGACATAGGTTATGATTTGCCTGTACCTTCACAGCGAGAACCAATAAAACAGGATATCCATGAAGCAACCCATGGATATAAAGACATTACTACCTCATCTCTACATAATTTTAATTCTGAGAAGATTGAAAAAGCAACTTCTGGATTGCCTTTTGCAAAGGCAGATCCCGAATTCAGAAATGTACCTAAAAATGATATGCCATATTCAGATTCTGTACGAAGTGACCTATTGGAGTCATTCCAACAGTTTTTGGTGAAGCCACCTGTGCCTTCAAGATATAGCAGTGCAGTTTCTTCTGTAACCAACCCAACAATTTCCATGTCTGACGCTCTGAGACCAGGCACCGTACCTTTGTCTGAGGCATGTTCTGTACTTCCTCAGCGTCATACATCCCGTTCATACGCTCAACCTACCCTTTCTTACAAACAGTTAGCAAACAGTATGGTCTATAAACCTTCCATGCCACAGGCTCAGAACTACTCGCCCGTGCCACCAAATTTGCAGCAAGAGTATGTAGGTGGTAGTGTATTCCATCAGTCAGGGGCTAGCATGGAATACAACTTCCCACAGTATAGTGGAGCCTCAGTAAGCAGATTGCCTACATCTGCTGCTGCTGCTGTCACTGGTCATGTAAGTTTTGGGGCCTCTAATAACACTCACGGGAGTTTATTGCATAATGCATCTGCCACTCCTGGCAGCATACCCGACTATGATGATGTATTCCTCCCCCAGTATAAAGATGGAAACCATTTAGCCACTCTTTATCAGAGTTCAAGAACATTGCCTTCCGTTCCAGACAGTGCATTTAATAACTTACTTGGGCATAATCAACAGCATGCAGGATATCGACAAGGGCGGGCTGGACAGCTGCCCACCAAGTTGCAGAATTATCGGGGACACCCCGATTTCTATTTTTCCCAAAAGGACGTAACACAAGAACATCAGCGACAACGCCTCGATGATTTAGGCTTAGGAGGATTCCAAGACCTGTCACCTCAGCAGCTGCACCAGATTTGGCAGCGGACCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

803

Amino Acids

88.07

Weight (kDa)

6.88

Isoelectric Point (pI)

56.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 17 - 74 2.3e-28 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1138
Acc36I ACCTGC 1 cut(s) 254
AccB1I GGYRCC 2 cut(s) 1199, 1718
AccBSI CCGCTC 1 cut(s) 1304
AccI GTMKAC 2 cut(s) 583, 970
AccII CGCG 1 cut(s) 250
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 8 cut(s) 35, 38, 50, 515, 983, 1302, 2233, 2398
AclWI GGATC 5 cut(s) 325, 1279, 1347, 1360, 1535
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 4 cut(s) 1321, 1343, 1547, 1873
AcuI CTGAAG 2 cut(s) 101, 384
AcvI CACGTG 1 cut(s) 667
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 8 cut(s) 301, 466, 692, 1401, 1560, 1593, 1725, 1749
AfiI CCNNNNNNNGG 3 cut(s) 515, 546, 596
AgsI TTSAA 7 cut(s) 270, 558, 645, 1255, 1505, 1650, 2151
AhdI GACNNNNNGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 478, 1714, 2068
AjuI GAANNNNNNNTTGG 2 cut(s) 1247, 1279
AluBI AGCT 7 cut(s) 343, 404, 806, 936, 1082, 2245, 2380
AluI AGCT 7 cut(s) 343, 404, 806, 936, 1082, 2245, 2380
Alw26I GTCTC 4 cut(s) 5, 224, 496, 1705
AlwI GGATC 5 cut(s) 325, 1279, 1347, 1360, 1535
AlwNI CAGNNNCTG 6 cut(s) 79, 166, 494, 1085, 1589, 2380
Ama87I CYCGRG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 3 cut(s) 476, 561, 2019
ApoI RAATTY 4 cut(s) 1321, 1343, 1547, 1873
Asp700I GAANNNNTTC 1 cut(s) 767
AspLEI GCGC 3 cut(s) 43, 165, 1139
AspS9I GGNCC 4 cut(s) 142, 895, 2019, 2400
AsuHPI GGTGA 4 cut(s) 381, 494, 1642, 2361
AsuNHI GCTAGC 1 cut(s) 1922
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 3 cut(s) 142, 895, 2400
BamHI GGATCC 1 cut(s) 1352
BanI GGYRCC 2 cut(s) 1199, 1718
BauI CACGAG 1 cut(s) 717
BbrPI CACGTG 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 1017
BbvCI CCTCAGC 2 cut(s) 1755, 2373
BccI CCATC 5 cut(s) 303, 973, 1249, 1917, 2111
BceAI ACGGC 1 cut(s) 140
BcgI CGANNNNNNTGC 4 cut(s) 1116, 1150, 2249, 2283
BciT130I CCWGG 3 cut(s) 480, 1716, 2070
BciVI GTATCC 1 cut(s) 1306
BcoDI GTCTC 4 cut(s) 5, 224, 496, 1705
BfaI CTAG 5 cut(s) 282, 591, 1035, 1368, 1923
BfmI CTRYAG 1 cut(s) 1278
BfoI RGCGCY 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 254
BfuI GTATCC 1 cut(s) 1306
BglII AGATCT 1 cut(s) 111
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 3 cut(s) 480, 1716, 2070
Bme18I GGWCC 3 cut(s) 142, 895, 2400
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 4 cut(s) 142, 895, 2019, 2400
BmiI GGNNCC 9 cut(s) 105, 144, 424, 538, 1201, 1354, 1720, 1957, 2020
BmrFI CCNGG 3 cut(s) 480, 1716, 2070
BmrI ACTGGG 1 cut(s) 2101
BmsI GCATC 5 cut(s) 475, 506, 567, 625, 2066
BmtI GCTAGC 1 cut(s) 1926
BmuI ACTGGG 1 cut(s) 2101
BpiI GAAGAC 1 cut(s) 1017
BplI GAGNNNNNCTC 2 cut(s) 101, 133
Bpu10I CCTNAGC 3 cut(s) 1755, 2347, 2373
BsaAI YACGTR 2 cut(s) 605, 667
BsaBI GATNNNNATC 1 cut(s) 1122
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 1705
BsaJI CCNNGG 2 cut(s) 872, 1447
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 8 cut(s) 15, 45, 190, 220, 1275, 1305, 1947, 1977
Bsc4I CCNNNNNNNGG 3 cut(s) 515, 546, 596
Bse118I RCCGGY 1 cut(s) 1210
Bse1I ACTGG 7 cut(s) 307, 330, 748, 883, 966, 2005, 2107
Bse3DI GCAATG 2 cut(s) 952, 2156
Bse8I GATNNNNATC 1 cut(s) 1122
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 3 cut(s) 480, 1716, 2070
BseDI CCNNGG 2 cut(s) 872, 1447
BseGI GGATG 4 cut(s) 314, 640, 1123, 1766
BseJI GATNNNNATC 1 cut(s) 1122
BseLI CCNNNNNNNGG 3 cut(s) 515, 546, 596
BseMI GCAATG 2 cut(s) 952, 2156
BseNI ACTGG 7 cut(s) 307, 330, 748, 883, 966, 2005, 2107
BsgI GTGCAG 2 cut(s) 1683, 2366
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 3 cut(s) 478, 563, 2021
BshNI GGYRCC 2 cut(s) 1199, 1718
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 2 cut(s) 101, 1211
BslFI GGGAC 2 cut(s) 378, 2286
BslI CCNNNNNNNGG 3 cut(s) 515, 546, 596
BsmAI GTCTC 4 cut(s) 5, 224, 496, 1705
BsmBI CGTCTC 1 cut(s) 5
BsmFI GGGAC 2 cut(s) 378, 2286
BsmI GAATGC 1 cut(s) 1098
BsnI GGCC 3 cut(s) 478, 563, 2021
Bso31I GGTCTC 1 cut(s) 1705
BsoBI CYCGRG 1 cut(s) 145
Bsp13I TCCGGA 1 cut(s) 100
Bsp143I GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
Bsp19I CCATGG 1 cut(s) 1447
Bsp68I TCGCGA 1 cut(s) 250
BspACI CCGC 8 cut(s) 35, 38, 50, 515, 983, 1302, 2233, 2398
BspANI GGCC 3 cut(s) 478, 563, 2021
BspEI TCCGGA 1 cut(s) 100
BspFNI CGCG 1 cut(s) 250
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 9 cut(s) 105, 144, 424, 538, 1201, 1354, 1720, 1957, 2020
BspMI ACCTGC 1 cut(s) 254
BspOI GCTAGC 1 cut(s) 1926
BspPI GGATC 5 cut(s) 325, 1279, 1347, 1360, 1535
BspQI GCTCTTC 1 cut(s) 1366
BspT107I GGYRCC 2 cut(s) 1199, 1718
BspTNI GGTCTC 1 cut(s) 1705
BsrBI CCGCTC 1 cut(s) 1304
BsrDI GCAATG 2 cut(s) 952, 2156
BsrFI RCCGGY 1 cut(s) 1210
BsrI ACTGG 7 cut(s) 307, 330, 748, 883, 966, 2005, 2107
BssAI RCCGGY 1 cut(s) 1210
BssECI CCNNGG 2 cut(s) 872, 1447
BssMI GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 717
BssT1I CCWWGG 2 cut(s) 872, 1447
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 3 cut(s) 480, 1716, 2070
Bst4CI ACNGT 8 cut(s) 98, 1087, 1623, 1723, 1806, 1817, 1947, 2177
Bst6I CTCTTC 1 cut(s) 1366
BstACI GRCGYC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 2 cut(s) 605, 667
BstC8I GCNNGC 8 cut(s) 167, 296, 434, 1238, 1306, 1924, 2214, 2235
BstDSI CCRYGG 1 cut(s) 1447
BstF5I GGATG 4 cut(s) 314, 640, 1123, 1766
BstFNI CGCG 1 cut(s) 250
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 3 cut(s) 43, 165, 1139
BstKTI GATC 6 cut(s) 114, 320, 1126, 1274, 1355, 1543
BstMAI GTCTC 4 cut(s) 5, 224, 496, 1705
BstMBI GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
BstMWI GCNNNNNNNGC 5 cut(s) 49, 494, 942, 1642, 1991
BstNI CCWGG 3 cut(s) 480, 1716, 2070
BstNSI RCATGY 3 cut(s) 788, 1743, 2216
BstSCI CCNGG 3 cut(s) 478, 1714, 2068
BstSFI CTRYAG 1 cut(s) 1278
BstSNI TACGTA 1 cut(s) 605
BstUI CGCG 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 4 cut(s) 111, 1271, 1352, 1540
BstYI RGATCY 4 cut(s) 111, 1271, 1352, 1540
BsuI GTATCC 1 cut(s) 1306
BsuRI GGCC 3 cut(s) 478, 563, 2021
BtgI CCRYGG 1 cut(s) 1447
BtsCI GGATG 4 cut(s) 314, 640, 1123, 1766
BtsI GCAGTG 2 cut(s) 360, 1666
BtsIMutI CAGTG 4 cut(s) 360, 1666, 1998, 2182
BtuMI TCGCGA 1 cut(s) 250
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 8 cut(s) 167, 296, 434, 1238, 1306, 1924, 2214, 2235
CaiI CAGNNNCTG 6 cut(s) 79, 166, 494, 1085, 1589, 2380
CciI TCATGA 1 cut(s) 187
CfoI GCGC 3 cut(s) 43, 165, 1139
Cfr10I RCCGGY 1 cut(s) 1210
Cfr13I GGNCC 4 cut(s) 142, 895, 2019, 2400
CseI GACGC 3 cut(s) 161, 1712, 1748
Csp6I GTAC 8 cut(s) 300, 465, 691, 1400, 1559, 1592, 1724, 1748
CspCI CAANNNNNGTGG 2 cut(s) 1857, 1892
CviQI GTAC 8 cut(s) 300, 465, 691, 1400, 1559, 1592, 1724, 1748
DpnI GATC 6 cut(s) 113, 319, 1125, 1273, 1354, 1542
DpnII GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
DraI TTTAAA 1 cut(s) 828
DriI GACNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 1366
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 1366
EciI GGCGGA 1 cut(s) 39
Eco105I TACGTA 1 cut(s) 605
Eco130I CCWWGG 2 cut(s) 872, 1447
Eco31I GGTCTC 1 cut(s) 1705
Eco32I GATATC 2 cut(s) 1432, 2222
Eco47I GGWCC 3 cut(s) 142, 895, 2400
Eco57I CTGAAG 2 cut(s) 101, 384
Eco72I CACGTG 1 cut(s) 667
Eco88I CYCGRG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 2019
EcoRI GAATTC 1 cut(s) 1547
EcoRII CCWGG 3 cut(s) 478, 1714, 2068
EcoRV GATATC 2 cut(s) 1432, 2222
EcoT14I CCWWGG 2 cut(s) 872, 1447
EcoT22I ATGCAT 2 cut(s) 640, 2059
ErhI CCWWGG 2 cut(s) 872, 1447
Esp3I CGTCTC 1 cut(s) 5
FaqI GGGAC 2 cut(s) 378, 2286
FauI CCCGC 2 cut(s) 508, 2226
FauNDI CATATG 1 cut(s) 1063
FblI GTMKAC 2 cut(s) 583, 970
FokI GGATG 4 cut(s) 321, 647, 1130, 1753
FspBI CTAG 5 cut(s) 282, 591, 1035, 1368, 1923
FspI TGCGCA 1 cut(s) 1138
GlaI GCGC 3 cut(s) 42, 164, 1138
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 3 cut(s) 478, 563, 2021
HapII CCGG 2 cut(s) 101, 1211
HgaI GACGC 3 cut(s) 161, 1712, 1748
HhaI GCGC 3 cut(s) 43, 165, 1139
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 3 cut(s) 41, 163, 1137
HinP1I GCGC 3 cut(s) 41, 163, 1137
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 2 cut(s) 101, 1211
HphI GGTGA 4 cut(s) 381, 494, 1642, 2361
Hpy166II GTNNAC 4 cut(s) 94, 256, 584, 971
Hpy8I GTNNAC 4 cut(s) 94, 256, 584, 971
Hpy99I CGWCG 4 cut(s) 58, 61, 155, 1343
HpyCH4III ACNGT 8 cut(s) 98, 1087, 1623, 1723, 1806, 1817, 1947, 2177
HpyCH4IV ACGT 5 cut(s) 205, 604, 666, 867, 2304
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 494, 942, 1642, 1991
HpySE526I ACGT 5 cut(s) 205, 604, 666, 867, 2304
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 3 cut(s) 41, 163, 1137
Kpn2I TCCGGA 1 cut(s) 100
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
LguI GCTCTTC 1 cut(s) 1366
LmnI GCTCC 5 cut(s) 6, 103, 198, 388, 1955
LweI GCATC 5 cut(s) 475, 506, 567, 625, 2066
MaeI CTAG 5 cut(s) 282, 591, 1035, 1368, 1923
MaeII ACGT 5 cut(s) 205, 604, 666, 867, 2304
MaeIII GTNAC 8 cut(s) 732, 885, 985, 1598, 1675, 1996, 2305, 2367
MalI GATC 6 cut(s) 113, 319, 1125, 1273, 1354, 1542
MbiI CCGCTC 1 cut(s) 1304
MboI GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
MflI RGATCY 4 cut(s) 111, 1271, 1352, 1540
MlyI GAGTC 1 cut(s) 1619
MmeI TCCRAC 2 cut(s) 888, 1642
Mph1103I ATGCAT 2 cut(s) 640, 2059
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 767
MseI TTAA 6 cut(s) 93, 126, 573, 827, 1488, 2184
MslI CAYNNNNRTG 2 cut(s) 633, 652
MspA1I CMGCKG 4 cut(s) 806, 2245, 2380, 2398
MspI CCGG 2 cut(s) 101, 1211
MspR9I CCNGG 3 cut(s) 480, 1716, 2070
Mva1269I GAATGC 1 cut(s) 1098
MvaI CCWGG 3 cut(s) 480, 1716, 2070
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 5 cut(s) 49, 494, 942, 1642, 1991
NcoI CCATGG 1 cut(s) 1447
NdeI CATATG 1 cut(s) 1063
NdeII GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
NheI GCTAGC 1 cut(s) 1922
NlaIV GGNNCC 9 cut(s) 105, 144, 424, 538, 1201, 1354, 1720, 1957, 2020
NmuCI GTSAC 4 cut(s) 885, 1598, 1996, 2367
NruI TCGCGA 1 cut(s) 250
NsbI TGCGCA 1 cut(s) 1138
NsiI ATGCAT 2 cut(s) 640, 2059
NspI RCATGY 3 cut(s) 788, 1743, 2216
PaeI GCATGC 1 cut(s) 2216
PagI TCATGA 1 cut(s) 187
PciSI GCTCTTC 1 cut(s) 1366
PctI GAATGC 1 cut(s) 1098
PdmI GAANNNNTTC 1 cut(s) 767
PfeI GAWTC 9 cut(s) 455, 550, 671, 860, 902, 1046, 1105, 1586, 2355
PleI GAGTC 1 cut(s) 1618
PmaCI CACGTG 1 cut(s) 667
PmlI CACGTG 1 cut(s) 667
PpsI GAGTC 1 cut(s) 1618
Ppu21I YACGTR 2 cut(s) 605, 667
Psp6I CCWGG 3 cut(s) 478, 1714, 2068
PspCI CACGTG 1 cut(s) 667
PspGI CCWGG 3 cut(s) 478, 1714, 2068
PspN4I GGNNCC 9 cut(s) 105, 144, 424, 538, 1201, 1354, 1720, 1957, 2020
PspPI GGNCC 4 cut(s) 142, 895, 2019, 2400
PstNI CAGNNNCTG 6 cut(s) 79, 166, 494, 1085, 1589, 2380
PsuI RGATCY 4 cut(s) 111, 1271, 1352, 1540
PvuII CAGCTG 3 cut(s) 806, 2245, 2380
RruI TCGCGA 1 cut(s) 250
RsaI GTAC 8 cut(s) 301, 466, 692, 1401, 1560, 1593, 1725, 1749
RsaNI GTAC 8 cut(s) 300, 465, 691, 1400, 1559, 1592, 1724, 1748
RseI CAYNNNNRTG 2 cut(s) 633, 652
SapI GCTCTTC 1 cut(s) 1366
SaqAI TTAA 6 cut(s) 93, 126, 573, 827, 1488, 2184
Sau3AI GATC 6 cut(s) 111, 317, 1123, 1271, 1352, 1540
Sau96I GGNCC 4 cut(s) 142, 895, 2019, 2400
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 1 cut(s) 1619
ScrFI CCNGG 3 cut(s) 480, 1716, 2070
SfaNI GCATC 5 cut(s) 475, 506, 567, 625, 2066
SfcI CTRYAG 1 cut(s) 1278
SinI GGWCC 3 cut(s) 142, 895, 2400
SmiMI CAYNNNNRTG 2 cut(s) 633, 652
SnaBI TACGTA 1 cut(s) 605
SphI GCATGC 1 cut(s) 2216
SsiI CCGC 8 cut(s) 35, 38, 50, 515, 983, 1302, 2233, 2398
SspI AATATT 1 cut(s) 916
SspMI CTAG 5 cut(s) 282, 591, 1035, 1368, 1923
StyD4I CCNGG 3 cut(s) 478, 1714, 2068
StyI CCWWGG 2 cut(s) 872, 1447
TaaI ACNGT 8 cut(s) 98, 1087, 1623, 1723, 1806, 1817, 1947, 2177
TaiI ACGT 5 cut(s) 208, 607, 669, 870, 2307
TaqI TCGA 4 cut(s) 1126, 1232, 2224, 2334
TatI WGTACW 2 cut(s) 464, 1747
TauI GCSGC 3 cut(s) 38, 41, 1304
TfiI GAWTC 9 cut(s) 455, 550, 671, 860, 902, 1046, 1105, 1586, 2355
Tru1I TTAA 6 cut(s) 93, 126, 573, 827, 1488, 2184
Tru9I TTAA 6 cut(s) 93, 126, 573, 827, 1488, 2184
TscAI CASTG 4 cut(s) 360, 1666, 2005, 2182
TseFI GTSAC 4 cut(s) 885, 1598, 1996, 2367
Tsp45I GTSAC 4 cut(s) 885, 1598, 1996, 2367
TspGWI ACGGA 1 cut(s) 2155
TspRI CASTG 4 cut(s) 360, 1666, 2005, 2182
VpaK11BI GGWCC 3 cut(s) 142, 895, 2400
XapI RAATTY 4 cut(s) 1321, 1343, 1547, 1873
XceI RCATGY 3 cut(s) 788, 1743, 2216
XcmI CCANNNNNNNNNTGG 1 cut(s) 2114
XmiI GTMKAC 2 cut(s) 583, 970
XmnI GAANNNNTTC 1 cut(s) 767
XspI CTAG 5 cut(s) 282, 591, 1035, 1368, 1923
ZrmI AGTACT 1 cut(s) 466
Zsp2I ATGCAT 2 cut(s) 640, 2059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.