pycom17g15570

V-type proton ATPase catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008079
Physical Location & Seq
Forward (+)
13613332 .. 13618305
4974 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1872 bp
ATGCCGGCGGTGTACGGTGCCCGATTGACTACATTTGAGGACTCCGAGAAGGAGAGCGAGTATGGCTACGTCCGCAAGGTCTCTGGACCAGTCGTGGTTGCCGATGGCATGGCTGGAGCTGCTATGTATGAACTAGTTCGTGTTGGACATGATAATTTGATCGGTGAAATTATTCGGTTAGAAGGAGATTCTGCCACAATACAAGTTTATGAGGAAACAGCTGGGTTGATGGTGATCGATCCGGTTCTACGTACTCGCAAGCCTTTATCAGTGGAGTTGGGACCTGGGATATTGGGAAATATTTTTGATGGAATCCAGAGGCCCTTGAAAACTATTGCAAAGATATCTGGTGATGTCTATATCCCACGTGGTGTATCTGTCCCAGCTCTTGACAAAGATATACTATGGGAGTTTCAGCCAAAAAAAATAGGTGAAGGTGATCATCTTACCGGTGGAGACTTGTATGCTACTGTCTTCGAAAATAGTCTGATGGAGCACCGAGTTGCACTTCCTCCTGATGCTATGGGAAAAGTTACTTATGTCGCACCACCTGGTCAATATTCATTGAAGGATACTGTATTAGAGCTTGAGTTTCAAGGACAAAAAAAGAAATTTACTATGCTTCAGACTTGGCCTGTACGTACACCTAGGCCTGTTGCAGCAAAGGTTGCTGCTGATACCCCTCTACTCACTGGGCAGCGCGTTCTTGATGCCCTTTTCCCCTCTGTTCTTGGTGGGACTTGTGCCATTCCTGGGGCTTTTGGTTGTGGAAAAACGGTTATTAGTCAAGCTCTTTCAAAGTACTCGAACTCAGACACTGTGGTTTATGTTGGTTGTGGAGAACGTGGAAATGAAATGGCAGAGGTGCTTATGGATTTCCCTCAATTGACAATGACTTTGCCTGATGGTCGTGAAGAATCTGTCATGAAGCGCACAACACTTGTGGCCAACACTTCTAACATGCCTGTGGCTGCTCGTGAAGCTTCAATCTACACTGGAATTACTATTGCTGAATACTTCAGAGATATGGGCTACAATGTCAGCATGATGGCAGATTCAACATCTCGATGGGCCGAGGCATTGCGTGAGATTTCTGGGCGGCTGGCTGAAATGCCTGCAGATAGTGGATACCCTGCTTATTTGGCAGCACGTTTAGCATCATTTTACGAACGGGCGGGTAAAGTAAAATGTCTTGGTGGGCCAGAGCGTAATGGCAGTGTGACAATTGTTGGTGCTGTTTCTCCTCCAGGAGGAGATTTCTCAGACCCTGTGACATCTGCAACACTTAGCATTGTTCAGGTTTTCTGGGGTCTGGACAAAAAACTTGCTCAGAGGAAGCATTTCCCCTCTGTGAACTGGCTTATTTCCTACTCAAAGTACTCAACAGCATTAGAGTCTTTCTATGATCAATTTGATCCTGATTTTATCAACATCAGGACAAAGGCACGTGAGGTTTTGCAGCGAGAAGATGATCTCAATGAAATAGTCCAACTTGTAGGAAAGGATGCTTTGGCTGAAGGAGATAAGATCACCTTAGAGACTGCAAAGCTCTTGAGGGAGGATTATCTTGCACAGAACGCATTTACTCCATATGACAAGTTCTGCCCATTCTACAAGTCCGTCTGGATGATGCGGAATATCATCCATTACTTTAATCTGGCCAATCAGGCTGTAGAGAGAGCCGCTGGCATGGATGGTCAAAAGATAAGTTACAGCCTAATCAAGCACCGTTTGGGAGATCTCTTTTACCGCCTAGTGTCTCAAAAGTTTGAGGATCCGGCTGAAGGGGAAGAAGCTCTTGTGGCAAAATTTAAGAAGCTACACGAGGACCTCACAAGTGGTTTCCGTGCTCTTGAGGATGAAACCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0002020 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0006996 GO:0007030 GO:0007275 GO:0008150 GO:0008324 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009555 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010256 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019899 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0034220 GO:0036442 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0046961 GO:0048046 GO:0048229 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071840 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N ATP-synt_ab_Xtn

Protein Analysis

624

Amino Acids

68.75

Weight (kDa)

5.37

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 25 - 85 5.3e-14 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab_Xtn PF16886 127 - 214 9.9e-29 ATPsynthase alpha/beta subunit barrel-sandwich domain
ATP-synt_ab PF00006 232 - 458 9e-75 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_VA_C PF22919 466 - 559 3.4e-28 C-terminal domain of V and A type ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 17
AccII CGCG 1 cut(s) 702
AciI CCGC 7 cut(s) 8, 73, 1099, 1175, 1633, 1683, 1750
AclWI GGATC 4 cut(s) 233, 1409, 1769, 1782
AcoI YGGCCR 2 cut(s) 945, 1659
AcsI RAATTY 2 cut(s) 611, 1808
AcuI CTGAAG 4 cut(s) 608, 1003, 1536, 1803
AcvI CACGTG 2 cut(s) 368, 1448
AdeI CACNNNGTG 1 cut(s) 371
AfaI GTAC 6 cut(s) 14, 253, 639, 643, 803, 1379
AfiI CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 6 cut(s) 328, 568, 596, 798, 987, 1059
AhlI ACTAGT 1 cut(s) 133
AjnI CCWGG 4 cut(s) 283, 550, 751, 1246
AluBI AGCT 9 cut(s) 119, 221, 386, 586, 791, 983, 1549, 1796, 1819
AluI AGCT 9 cut(s) 119, 221, 386, 586, 791, 983, 1549, 1796, 1819
Alw21I GWGCWC 2 cut(s) 498, 1852
Alw26I GTCTC 4 cut(s) 85, 450, 1532, 1764
AlwI GGATC 4 cut(s) 233, 1409, 1769, 1782
AlwNI CAGNNNCTG 2 cut(s) 818, 1268
AoxI GGCC 7 cut(s) 320, 632, 650, 945, 1071, 1199, 1659
ApeKI GCWGC 7 cut(s) 119, 659, 671, 697, 971, 1145, 1459
ApoI RAATTY 2 cut(s) 611, 1808
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AsiGI ACCGGT 1 cut(s) 449
Asp700I GAANNNNTTC 3 cut(s) 135, 171, 1340
AspA2I CCTAGG 1 cut(s) 647
AspLEI GCGC 2 cut(s) 702, 933
AspS9I GGNCC 6 cut(s) 86, 281, 321, 1071, 1199, 1828
AsuC2I CCSGG 1 cut(s) 1867
AsuHPI GGTGA 6 cut(s) 176, 244, 362, 443, 449, 1522
AsuII TTCGAA 1 cut(s) 477
AvaII GGWCC 3 cut(s) 86, 281, 1828
AvrII CCTAGG 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 22
BalI TGGCCA 2 cut(s) 947, 1661
BamHI GGATCC 1 cut(s) 1774
BanI GGYRCC 1 cut(s) 17
BauI CACGAG 2 cut(s) 975, 1823
BbrPI CACGTG 2 cut(s) 368, 1448
BbsI GAAGAC 1 cut(s) 466
Bbv12I GWGCWC 2 cut(s) 498, 1852
BbvI GCAGC 7 cut(s) 106, 658, 671, 709, 958, 1157, 1471
BccI CCATC 8 cut(s) 98, 223, 302, 484, 899, 1042, 1062, 1688
BciT130I CCWGG 4 cut(s) 285, 552, 753, 1248
BciVI GTATCC 2 cut(s) 565, 1121
BclI TGATCA 2 cut(s) 439, 1405
BcnI CCSGG 1 cut(s) 1867
BcoDI GTCTC 4 cut(s) 85, 450, 1532, 1764
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 3 cut(s) 134, 648, 1754
BfmI CTRYAG 2 cut(s) 1116, 1671
BfuI GTATCC 2 cut(s) 565, 1121
BglI GCCNNNNNGGC 1 cut(s) 1667
BglII AGATCT 1 cut(s) 1738
BisI GCNGC 9 cut(s) 120, 660, 672, 698, 972, 1100, 1146, 1460, 1683
BlnI CCTAGG 1 cut(s) 647
BlsI GCNGC 9 cut(s) 121, 661, 673, 699, 973, 1101, 1147, 1461, 1684
BmcAI AGTACT 2 cut(s) 803, 1379
Bme1390I CCNGG 5 cut(s) 285, 552, 753, 1248, 1867
Bme18I GGWCC 3 cut(s) 86, 281, 1828
BmgT120I GGNCC 6 cut(s) 86, 281, 321, 1071, 1199, 1828
BmiI GGNNCC 3 cut(s) 19, 282, 1776
BmrFI CCNGG 5 cut(s) 285, 552, 753, 1248, 1867
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 5 cut(s) 508, 700, 1166, 1495, 1620
BmuI ACTGGG 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 2 cut(s) 135, 1230
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 2 cut(s) 608, 1573
BpuMI CCSGG 1 cut(s) 1867
Bsa29I ATCGAT 1 cut(s) 237
BsaAI YACGTR 4 cut(s) 251, 368, 641, 1448
BsaBI GATNNNNATC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 4 cut(s) 284, 647, 752, 1074
BsaWI WCCGGW 2 cut(s) 241, 449
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
Bse118I RCCGGY 2 cut(s) 4, 449
Bse1I ACTGG 4 cut(s) 89, 697, 1000, 1361
Bse3DI GCAATG 1 cut(s) 1079
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 4 cut(s) 285, 552, 753, 1248
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 4 cut(s) 284, 647, 752, 1074
BseGI GGATG 5 cut(s) 1510, 1632, 1641, 1699, 1864
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 3 cut(s) 825, 1275, 1343
BseNI ACTGG 4 cut(s) 89, 697, 1000, 1361
BseRI GAGGAG 2 cut(s) 1233, 1266
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 7 cut(s) 106, 658, 671, 709, 958, 1157, 1471
BseYI CCCAGC 2 cut(s) 221, 382
Bsh1236I CGCG 1 cut(s) 702
BshFI GGCC 7 cut(s) 322, 634, 652, 947, 1073, 1201, 1661
BshNI GGYRCC 1 cut(s) 17
BshTI ACCGGT 1 cut(s) 449
BshVI ATCGAT 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 498, 1852
BsiSI CCGG 5 cut(s) 5, 242, 450, 1778, 1867
BslFI GGGAC 3 cut(s) 294, 365, 751
BslI CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
BsmAI GTCTC 4 cut(s) 85, 450, 1532, 1764
BsmFI GGGAC 3 cut(s) 294, 365, 751
BsnI GGCC 7 cut(s) 322, 634, 652, 947, 1073, 1201, 1661
Bso31I GGTCTC 1 cut(s) 85
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 3 cut(s) 22, 498, 1852
BspACI CCGC 7 cut(s) 8, 73, 1099, 1175, 1633, 1683, 1750
BspANI GGCC 7 cut(s) 322, 634, 652, 947, 1073, 1201, 1661
BspCNI CTCAG 3 cut(s) 824, 1274, 1342
BspDI ATCGAT 1 cut(s) 237
BspFNI CGCG 1 cut(s) 702
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 3 cut(s) 19, 282, 1776
BspMAI CTGCAG 1 cut(s) 1120
BspPI GGATC 4 cut(s) 233, 1409, 1769, 1782
BspT104I TTCGAA 1 cut(s) 477
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 2 cut(s) 4, 449
BsrI ACTGG 4 cut(s) 89, 697, 1000, 1361
BssAI RCCGGY 2 cut(s) 4, 449
BssECI CCNNGG 4 cut(s) 284, 647, 752, 1074
BssSI CACGAG 2 cut(s) 975, 1823
BssT1I CCWWGG 1 cut(s) 647
Bst2BI CACGAG 2 cut(s) 975, 1823
Bst2UI CCWGG 4 cut(s) 285, 552, 753, 1248
Bst4CI ACNGT 6 cut(s) 17, 472, 577, 778, 820, 1730
BstAPI GCANNNNNTGC 1 cut(s) 668
BstBAI YACGTR 4 cut(s) 251, 368, 641, 1448
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 5 cut(s) 6, 260, 1104, 1116, 1687
BstDEI CTNAG 5 cut(s) 811, 1261, 1286, 1329, 1534
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 5 cut(s) 1510, 1632, 1641, 1699, 1864
BstFNI CGCG 1 cut(s) 702
BstHHI GCGC 2 cut(s) 702, 933
BstMAI GTCTC 4 cut(s) 85, 450, 1532, 1764
BstNI CCWGG 4 cut(s) 285, 552, 753, 1248
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 5 cut(s) 283, 550, 751, 1246, 1865
BstSFI CTRYAG 2 cut(s) 1116, 1671
BstSLI GKGCMC 1 cut(s) 22
BstSNI TACGTA 2 cut(s) 251, 641
BstUI CGCG 1 cut(s) 702
BstV1I GCAGC 7 cut(s) 106, 658, 671, 709, 958, 1157, 1471
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 2 cut(s) 1738, 1774
BstYI RGATCY 2 cut(s) 1738, 1774
Bsu15I ATCGAT 1 cut(s) 237
BsuI GTATCC 2 cut(s) 565, 1121
BsuRI GGCC 7 cut(s) 322, 634, 652, 947, 1073, 1201, 1661
BsuTUI ATCGAT 1 cut(s) 237
BtsCI GGATG 5 cut(s) 1510, 1632, 1641, 1699, 1864
BtsI GCAGTG 1 cut(s) 1222
BtsIMutI CAGTG 5 cut(s) 276, 690, 816, 993, 1222
Cac8I GCNNGC 5 cut(s) 6, 260, 1104, 1116, 1687
CaiI CAGNNNCTG 2 cut(s) 818, 1268
CciI TCATGA 1 cut(s) 924
CfoI GCGC 2 cut(s) 702, 933
Cfr10I RCCGGY 2 cut(s) 4, 449
Cfr13I GGNCC 6 cut(s) 86, 281, 321, 1071, 1199, 1828
ClaI ATCGAT 1 cut(s) 237
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 6 cut(s) 13, 252, 638, 642, 802, 1378
CspAI ACCGGT 1 cut(s) 449
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CviAII CATG 6 cut(s) 109, 149, 925, 961, 1045, 1690
CviQI GTAC 6 cut(s) 13, 252, 638, 642, 802, 1378
DdeI CTNAG 5 cut(s) 811, 1261, 1286, 1329, 1534
DraIII CACNNNGTG 1 cut(s) 371
EaeI YGGCCR 2 cut(s) 945, 1659
Eco105I TACGTA 2 cut(s) 251, 641
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 85
Eco32I GATATC 1 cut(s) 345
Eco47I GGWCC 3 cut(s) 86, 281, 1828
Eco57I CTGAAG 4 cut(s) 608, 1003, 1536, 1803
Eco72I CACGTG 2 cut(s) 368, 1448
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoO109I RGGNCCY 3 cut(s) 281, 321, 1828
EcoRII CCWGG 4 cut(s) 283, 550, 751, 1246
EcoRV GATATC 1 cut(s) 345
EcoT14I CCWWGG 1 cut(s) 647
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 6 cut(s) 112, 152, 928, 964, 1048, 1693
FalI AAGNNNNNCTT 4 cut(s) 1517, 1549, 1782, 1814
FaqI GGGAC 3 cut(s) 294, 365, 751
FatI CATG 6 cut(s) 108, 148, 924, 960, 1044, 1689
FauI CCCGC 1 cut(s) 1168
FauNDI CATATG 1 cut(s) 1591
FbaI TGATCA 2 cut(s) 439, 1405
Fnu4HI GCNGC 9 cut(s) 120, 660, 672, 698, 972, 1100, 1146, 1460, 1683
FokI GGATG 4 cut(s) 1517, 1628, 1639, 1706
Fsp4HI GCNGC 9 cut(s) 120, 660, 672, 698, 972, 1100, 1146, 1460, 1683
FspBI CTAG 3 cut(s) 134, 648, 1754
GlaI GCGC 2 cut(s) 701, 932
GluI GCNGC 9 cut(s) 120, 660, 672, 698, 972, 1100, 1146, 1460, 1683
GsaI CCCAGC 2 cut(s) 225, 386
GsuI CTGGAG 2 cut(s) 135, 1230
HaeIII GGCC 7 cut(s) 322, 634, 652, 947, 1073, 1201, 1661
HapII CCGG 5 cut(s) 5, 242, 450, 1778, 1867
HhaI GCGC 2 cut(s) 702, 933
Hin1II CATG 6 cut(s) 112, 152, 928, 964, 1048, 1693
Hin6I GCGC 2 cut(s) 700, 931
HinP1I GCGC 2 cut(s) 700, 931
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 6 cut(s) 41, 188, 312, 917, 1055, 1394
HpaII CCGG 5 cut(s) 5, 242, 450, 1778, 1867
HphI GGTGA 6 cut(s) 176, 244, 362, 443, 449, 1522
Hpy166II GTNNAC 3 cut(s) 13, 644, 1354
Hpy188I TCNGA 7 cut(s) 46, 489, 627, 814, 1022, 1264, 1332
Hpy8I GTNNAC 3 cut(s) 13, 644, 1354
HpyAV CCTTC 6 cut(s) 43, 176, 428, 562, 1511, 1778
HpyCH4III ACNGT 6 cut(s) 17, 472, 577, 778, 820, 1730
HpyCH4IV ACGT 7 cut(s) 69, 250, 367, 640, 844, 1150, 1447
HpyCH4V TGCA 8 cut(s) 338, 506, 659, 1118, 1280, 1459, 1544, 1571
HpyF3I CTNAG 5 cut(s) 811, 1261, 1286, 1329, 1534
HpySE526I ACGT 7 cut(s) 69, 250, 367, 640, 844, 1150, 1447
Hsp92II CATG 6 cut(s) 112, 152, 928, 964, 1048, 1693
HspAI GCGC 2 cut(s) 700, 931
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 439, 1405
LmnI GCTCC 2 cut(s) 116, 493
Lsp1109I GCAGC 7 cut(s) 106, 658, 671, 709, 958, 1157, 1471
LweI GCATC 5 cut(s) 508, 700, 1166, 1495, 1620
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 3 cut(s) 134, 648, 1754
MaeII ACGT 7 cut(s) 69, 250, 367, 640, 844, 1150, 1447
MaeIII GTNAC 4 cut(s) 532, 1219, 1270, 1709
MboII GAAGA 4 cut(s) 466, 926, 1478, 1802
MfeI CAATTG 2 cut(s) 884, 1224
MflI RGATCY 2 cut(s) 1738, 1774
MhlI GDGCHC 3 cut(s) 22, 498, 1852
MlsI TGGCCA 2 cut(s) 947, 1661
MluCI AATT 8 cut(s) 154, 168, 611, 884, 999, 1224, 1409, 1808
MluNI TGGCCA 2 cut(s) 947, 1661
MlyI GAGTC 2 cut(s) 35, 1403
MmeI TCCRAC 2 cut(s) 124, 1513
Mox20I TGGCCA 2 cut(s) 947, 1661
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 3 cut(s) 135, 171, 1340
MscI TGGCCA 2 cut(s) 947, 1661
MseI TTAA 2 cut(s) 1653, 1812
MslI CAYNNNNRTG 2 cut(s) 965, 1066
Msp20I TGGCCA 2 cut(s) 947, 1661
MspA1I CMGCKG 2 cut(s) 221, 1685
MspI CCGG 5 cut(s) 5, 242, 450, 1778, 1867
MspR9I CCNGG 5 cut(s) 285, 552, 753, 1248, 1867
MunI CAATTG 2 cut(s) 884, 1224
MvaI CCWGG 4 cut(s) 285, 552, 753, 1248
MvnI CGCG 1 cut(s) 702
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 1867
NdeI CATATG 1 cut(s) 1591
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 112, 152, 928, 964, 1048, 1693
NlaIV GGNNCC 3 cut(s) 19, 282, 1776
NmeAIII GCCGAG 1 cut(s) 1099
NmuCI GTSAC 2 cut(s) 1219, 1270
NspI RCATGY 1 cut(s) 964
NspV TTCGAA 1 cut(s) 477
PagI TCATGA 1 cut(s) 924
PceI AGGCCT 1 cut(s) 652
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 3 cut(s) 135, 171, 1340
PfeI GAWTC 4 cut(s) 188, 312, 917, 1055
PfoI TCCNGGA 1 cut(s) 1246
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 9 cut(s) 121, 661, 673, 699, 973, 1101, 1147, 1461, 1684
PleI GAGTC 2 cut(s) 35, 1402
PmaCI CACGTG 2 cut(s) 368, 1448
PmlI CACGTG 2 cut(s) 368, 1448
PpsI GAGTC 2 cut(s) 35, 1402
Ppu21I YACGTR 4 cut(s) 251, 368, 641, 1448
PpuMI RGGWCCY 2 cut(s) 281, 1828
Psp5II RGGWCCY 2 cut(s) 281, 1828
Psp6I CCWGG 4 cut(s) 283, 550, 751, 1246
PspCI CACGTG 2 cut(s) 368, 1448
PspFI CCCAGC 2 cut(s) 221, 382
PspGI CCWGG 4 cut(s) 283, 550, 751, 1246
PspN4I GGNNCC 3 cut(s) 19, 282, 1776
PspPI GGNCC 6 cut(s) 86, 281, 321, 1071, 1199, 1828
PspPPI RGGWCCY 2 cut(s) 281, 1828
PstI CTGCAG 1 cut(s) 1120
PstNI CAGNNNCTG 2 cut(s) 818, 1268
PsuI RGATCY 2 cut(s) 1738, 1774
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 6 cut(s) 14, 253, 639, 643, 803, 1379
RsaNI GTAC 6 cut(s) 13, 252, 638, 642, 802, 1378
RseI CAYNNNNRTG 2 cut(s) 965, 1066
SaqAI TTAA 2 cut(s) 1653, 1812
SatI GCNGC 9 cut(s) 120, 660, 672, 698, 972, 1100, 1146, 1460, 1683
Sau96I GGNCC 6 cut(s) 86, 281, 321, 1071, 1199, 1828
ScaI AGTACT 2 cut(s) 803, 1379
SchI GAGTC 2 cut(s) 35, 1403
ScrFI CCNGG 5 cut(s) 285, 552, 753, 1248, 1867
SduI GDGCHC 3 cut(s) 22, 498, 1852
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 5 cut(s) 508, 700, 1166, 1495, 1620
SfcI CTRYAG 2 cut(s) 1116, 1671
SfuI TTCGAA 1 cut(s) 477
SinI GGWCC 3 cut(s) 86, 281, 1828
SmiMI CAYNNNNRTG 2 cut(s) 965, 1066
SmlI CTYRAG 3 cut(s) 587, 1552, 1853
SmoI CTYRAG 3 cut(s) 587, 1552, 1853
SnaBI TACGTA 2 cut(s) 251, 641
SpeI ACTAGT 1 cut(s) 133
Sse9I AATT 8 cut(s) 154, 168, 611, 884, 999, 1224, 1409, 1808
SseBI AGGCCT 1 cut(s) 652
SsiI CCGC 7 cut(s) 8, 73, 1099, 1175, 1633, 1683, 1750
SspI AATATT 2 cut(s) 301, 560
SspMI CTAG 3 cut(s) 134, 648, 1754
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 5 cut(s) 283, 550, 751, 1246, 1865
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 6 cut(s) 17, 472, 577, 778, 820, 1730
TaiI ACGT 7 cut(s) 72, 253, 370, 643, 847, 1153, 1450
TaqI TCGA 4 cut(s) 237, 477, 806, 1066
TasI AATT 8 cut(s) 154, 168, 611, 884, 999, 1224, 1409, 1808
TatI WGTACW 2 cut(s) 801, 1377
TauI GCSGC 2 cut(s) 1102, 1685
TfiI GAWTC 4 cut(s) 188, 312, 917, 1055
Tru1I TTAA 2 cut(s) 1653, 1812
Tru9I TTAA 2 cut(s) 1653, 1812
TscAI CASTG 5 cut(s) 276, 697, 823, 1000, 1222
TseFI GTSAC 2 cut(s) 1219, 1270
TseI GCWGC 7 cut(s) 119, 659, 671, 697, 971, 1145, 1459
Tsp45I GTSAC 2 cut(s) 1219, 1270
TspDTI ATGAA 5 cut(s) 144, 552, 867, 941, 1494
TspGWI ACGGA 2 cut(s) 1609, 1835
TspRI CASTG 5 cut(s) 276, 697, 823, 1000, 1222
VpaK11BI GGWCC 3 cut(s) 86, 281, 1828
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 2 cut(s) 611, 1808
XceI RCATGY 1 cut(s) 964
XmaJI CCTAGG 1 cut(s) 647
XmnI GAANNNNTTC 3 cut(s) 135, 171, 1340
XspI CTAG 3 cut(s) 134, 648, 1754
ZrmI AGTACT 2 cut(s) 803, 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.