NatA auxiliary
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G80410 AT1G80410 |
| fragaria_vesca | FvH4_6g30900 FvH4_6g30900 |
| malus_domestica | MD09G1215200.v1.1 MD17G1197900.v1.1 |
| prunus_persica | Prupe.3G074300_v2.0.a1 |
| pyrus_communis | pycom09g13200 pycom17g20280 |
| rosa_chinensis | RchiOBHm_Chr2g0137391 |
| rosa_laevigata | RLG00000019656 |
| rosa_multiflora | Rmu_ssc0000312.1_g000014 |
| rosa_roxburghii | Rroxscaffold_2G00107080 |
| rosa_rugosa | Rorug02G0342200 |
| rosa_samantha | Rh2AG390300 Rh2BG397700 Rh2CG377600 Rh2DG413000 |
| rosa_wichuraiana | Rw2G031980 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 675 |
| Acc16I | TGCGCA | 1 cut(s) | 876 |
| Acc36I | ACCTGC | 1 cut(s) | 1933 |
| AccB1I | GGYRCC | 1 cut(s) | 2395 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 125, 2152 |
| AccIII | TCCGGA | 1 cut(s) | 198 |
| AciI | CCGC | 8 cut(s) | 172, 373, 1504, 1688, 1776, 1922, 2312, 2492 |
| AclI | AACGTT | 1 cut(s) | 2515 |
| AclWI | GGATC | 8 cut(s) | 314, 1115, 1128, 1338, 1907, 1913, 1946, 1959 |
| AcsI | RAATTY | 7 cut(s) | 472, 498, 1535, 1643, 2138, 2269, 2656 |
| AcuI | CTGAAG | 4 cut(s) | 107, 201, 230, 1973 |
| AcyI | GRCGYC | 1 cut(s) | 99 |
| AfaI | GTAC | 7 cut(s) | 863, 1416, 1867, 1976, 2528, 2574, 2614 |
| AfeI | AGCGCT | 1 cut(s) | 2412 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 25, 125, 982, 1036, 1108, 1140, 1141, 2152 |
| AflII | CTTAAG | 1 cut(s) | 1603 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1092 |
| AjnI | CCWGG | 2 cut(s) | 1030, 1102 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2234, 2266, 2463, 2495 |
| Alw21I | GWGCWC | 2 cut(s) | 625, 1227 |
| Alw26I | GTCTC | 2 cut(s) | 389, 2009 |
| AlwI | GGATC | 8 cut(s) | 314, 1115, 1128, 1338, 1907, 1913, 1946, 1959 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2092 |
| Aor13HI | TCCGGA | 1 cut(s) | 198 |
| Aor51HI | AGCGCT | 1 cut(s) | 2412 |
| AoxI | GGCC | 7 cut(s) | 27, 784, 968, 1143, 1215, 2417, 2674 |
| ApoI | RAATTY | 7 cut(s) | 472, 498, 1535, 1643, 2138, 2269, 2656 |
| AseI | ATTAAT | 1 cut(s) | 2369 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 339, 2475 |
| AspA2I | CCTAGG | 1 cut(s) | 715 |
| AspLEI | GCGC | 3 cut(s) | 877, 2220, 2413 |
| AspS9I | GGNCC | 4 cut(s) | 169, 1143, 2417, 2683 |
| AvaII | GGWCC | 2 cut(s) | 169, 2683 |
| AvrII | CCTAGG | 1 cut(s) | 715 |
| BaeGI | GKGCMC | 1 cut(s) | 2400 |
| BamHI | GGATCC | 2 cut(s) | 1120, 1951 |
| BanI | GGYRCC | 1 cut(s) | 2395 |
| BanII | GRGCYC | 1 cut(s) | 625 |
| BbsI | GAAGAC | 1 cut(s) | 245 |
| Bbv12I | GWGCWC | 2 cut(s) | 625, 1227 |
| BbvCI | CCTCAGC | 1 cut(s) | 1796 |
| BccI | CCATC | 5 cut(s) | 471, 524, 1424, 2170, 2499 |
| BceAI | ACGGC | 1 cut(s) | 2555 |
| BciT130I | CCWGG | 2 cut(s) | 1032, 1104 |
| BclI | TGATCA | 4 cut(s) | 1024, 1384, 1435, 2226 |
| BcoDI | GTCTC | 2 cut(s) | 389, 2009 |
| BfaI | CTAG | 3 cut(s) | 524, 716, 1320 |
| BfmI | CTRYAG | 4 cut(s) | 298, 359, 951, 1376 |
| BfoI | RGCGCY | 2 cut(s) | 2221, 2414 |
| BfrI | CTTAAG | 1 cut(s) | 1603 |
| BfuAI | ACCTGC | 1 cut(s) | 1933 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2075 |
| BglII | AGATCT | 1 cut(s) | 1006 |
| BlnI | CCTAGG | 1 cut(s) | 715 |
| BlpI | GCTNAGC | 2 cut(s) | 1161, 2214 |
| BmcAI | AGTACT | 2 cut(s) | 1976, 2528 |
| Bme1390I | CCNGG | 2 cut(s) | 1032, 1104 |
| Bme18I | GGWCC | 2 cut(s) | 169, 2683 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1092 |
| BmgT120I | GGNCC | 4 cut(s) | 169, 1143, 2417, 2683 |
| BmiI | GGNNCC | 5 cut(s) | 7, 1122, 1392, 1953, 2397 |
| BmrFI | CCNGG | 2 cut(s) | 1032, 1104 |
| BmrI | ACTGGG | 1 cut(s) | 817 |
| BmsI | GCATC | 3 cut(s) | 561, 844, 1694 |
| BmuI | ACTGGG | 1 cut(s) | 817 |
| BpiI | GAAGAC | 1 cut(s) | 245 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 29 |
| BpmI | CTGGAG | 1 cut(s) | 525 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1796 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1161, 2214 |
| BpuEI | CTTGAG | 2 cut(s) | 647, 1085 |
| Bsa29I | ATCGAT | 2 cut(s) | 772, 1654 |
| BsaBI | GATNNNNATC | 3 cut(s) | 534, 1434, 1917 |
| BsaHI | GRCGYC | 1 cut(s) | 99 |
| BsaJI | CCNNGG | 7 cut(s) | 124, 715, 1030, 1102, 1103, 1507, 2010 |
| BsaWI | WCCGGW | 2 cut(s) | 198, 1487 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1922, 1952 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 25, 125, 982, 1036, 1108, 1140, 1141, 2152 |
| Bse1I | ACTGG | 4 cut(s) | 823, 1096, 1311, 1863 |
| Bse3DI | GCAATG | 1 cut(s) | 2640 |
| Bse8I | GATNNNNATC | 3 cut(s) | 534, 1434, 1917 |
| BseAI | TCCGGA | 1 cut(s) | 198 |
| BseBI | CCWGG | 2 cut(s) | 1032, 1104 |
| BseCI | ATCGAT | 2 cut(s) | 772, 1654 |
| BseDI | CCNNGG | 7 cut(s) | 124, 715, 1030, 1102, 1103, 1507, 2010 |
| BseGI | GGATG | 2 cut(s) | 859, 1742 |
| BseJI | GATNNNNATC | 3 cut(s) | 534, 1434, 1917 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 25, 125, 982, 1036, 1108, 1140, 1141, 2152 |
| BseMI | GCAATG | 1 cut(s) | 2640 |
| BseMII | CTCAG | 9 cut(s) | 302, 1017, 1175, 1787, 1995, 2084, 2205, 2346, 2514 |
| BseNI | ACTGG | 4 cut(s) | 823, 1096, 1311, 1863 |
| BseRI | GAGGAG | 1 cut(s) | 1005 |
| BseSI | GKGCMC | 1 cut(s) | 2400 |
| BsgI | GTGCAG | 2 cut(s) | 1287, 2652 |
| BshFI | GGCC | 7 cut(s) | 29, 786, 970, 1145, 1217, 2419, 2676 |
| BshNI | GGYRCC | 1 cut(s) | 2395 |
| BshVI | ATCGAT | 2 cut(s) | 772, 1654 |
| BsiHKAI | GWGCWC | 2 cut(s) | 625, 1227 |
| BsiSI | CCGG | 2 cut(s) | 199, 1488 |
| BsiWI | CGTACG | 1 cut(s) | 2612 |
| BslFI | GGGAC | 1 cut(s) | 96 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 25, 125, 982, 1036, 1108, 1140, 1141, 2152 |
| BsmAI | GTCTC | 2 cut(s) | 389, 2009 |
| BsmBI | CGTCTC | 1 cut(s) | 2009 |
| BsmFI | GGGAC | 1 cut(s) | 96 |
| BsmI | GAATGC | 3 cut(s) | 823, 1660, 2666 |
| BsnI | GGCC | 7 cut(s) | 29, 786, 970, 1145, 1217, 2419, 2676 |
| Bsp1286I | GDGCHC | 3 cut(s) | 625, 1227, 2400 |
| Bsp13I | TCCGGA | 1 cut(s) | 198 |
| Bsp1407I | TGTACA | 1 cut(s) | 861 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1161, 2214 |
| Bsp19I | CCATGG | 1 cut(s) | 124 |
| BspACI | CCGC | 8 cut(s) | 172, 373, 1504, 1688, 1776, 1922, 2312, 2492 |
| BspANI | GGCC | 7 cut(s) | 29, 786, 970, 1145, 1217, 2419, 2676 |
| BspCNI | CTCAG | 9 cut(s) | 303, 1016, 1174, 1788, 1996, 2085, 2206, 2347, 2515 |
| BspDI | ATCGAT | 2 cut(s) | 772, 1654 |
| BspEI | TCCGGA | 1 cut(s) | 198 |
| BspHI | TCATGA | 2 cut(s) | 343, 1453 |
| BspLI | GGNNCC | 5 cut(s) | 7, 1122, 1392, 1953, 2397 |
| BspMAI | CTGCAG | 2 cut(s) | 955, 1380 |
| BspMI | ACCTGC | 1 cut(s) | 1933 |
| BspPI | GGATC | 8 cut(s) | 314, 1115, 1128, 1338, 1907, 1913, 1946, 1959 |
| BspT107I | GGYRCC | 1 cut(s) | 2395 |
| BspTI | CTTAAG | 1 cut(s) | 1603 |
| BsrDI | GCAATG | 1 cut(s) | 2640 |
| BsrGI | TGTACA | 1 cut(s) | 861 |
| BsrI | ACTGG | 4 cut(s) | 823, 1096, 1311, 1863 |
| BssECI | CCNNGG | 7 cut(s) | 124, 715, 1030, 1102, 1103, 1507, 2010 |
| BssNI | GRCGYC | 1 cut(s) | 99 |
| BssT1I | CCWWGG | 4 cut(s) | 124, 715, 1507, 2010 |
| Bst2UI | CCWGG | 2 cut(s) | 1032, 1104 |
| Bst4CI | ACNGT | 8 cut(s) | 887, 1077, 1237, 1361, 1419, 2455, 2612, 2683 |
| Bst6I | CTCTTC | 2 cut(s) | 41, 2370 |
| BstACI | GRCGYC | 1 cut(s) | 99 |
| BstAFI | CTTAAG | 1 cut(s) | 1603 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 818 |
| BstAUI | TGTACA | 1 cut(s) | 861 |
| BstC8I | GCNNGC | 9 cut(s) | 239, 788, 1278, 1293, 1380, 1623, 1776, 2067, 2166 |
| BstDSI | CCRYGG | 1 cut(s) | 124 |
| BstEII | GGTNACC | 2 cut(s) | 710, 2483 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 980 |
| BstF5I | GGATG | 2 cut(s) | 859, 1742 |
| BstH2I | RGCGCY | 2 cut(s) | 2221, 2414 |
| BstHHI | GCGC | 3 cut(s) | 877, 2220, 2413 |
| BstMAI | GTCTC | 2 cut(s) | 389, 2009 |
| BstNI | CCWGG | 2 cut(s) | 1032, 1104 |
| BstNSI | RCATGY | 4 cut(s) | 245, 1280, 1471, 1670 |
| BstPI | GGTNACC | 2 cut(s) | 710, 2483 |
| BstSCI | CCNGG | 2 cut(s) | 1030, 1102 |
| BstSFI | CTRYAG | 4 cut(s) | 298, 359, 951, 1376 |
| BstSLI | GKGCMC | 1 cut(s) | 2400 |
| BstV2I | GAAGAC | 1 cut(s) | 245 |
| BstX2I | RGATCY | 5 cut(s) | 319, 1006, 1120, 1330, 1951 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2392 |
| BstYI | RGATCY | 5 cut(s) | 319, 1006, 1120, 1330, 1951 |
| Bsu15I | ATCGAT | 2 cut(s) | 772, 1654 |
| BsuRI | GGCC | 7 cut(s) | 29, 786, 970, 1145, 1217, 2419, 2676 |
| BsuTUI | ATCGAT | 2 cut(s) | 772, 1654 |
| BtgI | CCRYGG | 1 cut(s) | 124 |
| BtgZI | GCGATG | 2 cut(s) | 416, 2108 |
| BtsCI | GGATG | 2 cut(s) | 859, 1742 |
| BtsI | GCAGTG | 1 cut(s) | 1469 |
| BtsIMutI | CAGTG | 2 cut(s) | 1304, 1469 |
| BveI | ACCTGC | 1 cut(s) | 1933 |
| Cac8I | GCNNGC | 9 cut(s) | 239, 788, 1278, 1293, 1380, 1623, 1776, 2067, 2166 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2092 |
| CciI | TCATGA | 2 cut(s) | 343, 1453 |
| CfoI | GCGC | 3 cut(s) | 877, 2220, 2413 |
| Cfr13I | GGNCC | 4 cut(s) | 169, 1143, 2417, 2683 |
| ClaI | ATCGAT | 2 cut(s) | 772, 1654 |
| CseI | GACGC | 3 cut(s) | 107, 1624, 2026 |
| Csp6I | GTAC | 7 cut(s) | 862, 1415, 1866, 1975, 2527, 2573, 2613 |
| CviQI | GTAC | 7 cut(s) | 862, 1415, 1866, 1975, 2527, 2573, 2613 |
| DraI | TTTAAA | 2 cut(s) | 471, 486 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1092 |
| Eam1104I | CTCTTC | 2 cut(s) | 41, 2370 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1092 |
| EarI | CTCTTC | 2 cut(s) | 41, 2370 |
| EciI | GGCGGA | 1 cut(s) | 1493 |
| Ecl136II | GAGCTC | 1 cut(s) | 623 |
| Eco130I | CCWWGG | 4 cut(s) | 124, 715, 1507, 2010 |
| Eco147I | AGGCCT | 3 cut(s) | 786, 970, 2676 |
| Eco24I | GRGCYC | 1 cut(s) | 625 |
| Eco47I | GGWCC | 2 cut(s) | 169, 2683 |
| Eco47III | AGCGCT | 1 cut(s) | 2412 |
| Eco53kI | GAGCTC | 1 cut(s) | 623 |
| Eco57I | CTGAAG | 4 cut(s) | 107, 201, 230, 1973 |
| Eco91I | GGTNACC | 2 cut(s) | 710, 2483 |
| EcoICRI | GAGCTC | 1 cut(s) | 623 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 980 |
| EcoO65I | GGTNACC | 2 cut(s) | 710, 2483 |
| EcoRII | CCWGG | 2 cut(s) | 1030, 1102 |
| EcoT14I | CCWWGG | 4 cut(s) | 124, 715, 1507, 2010 |
| EcoT22I | ATGCAT | 2 cut(s) | 1672, 2304 |
| EcoT38I | GRGCYC | 1 cut(s) | 625 |
| ErhI | CCWWGG | 4 cut(s) | 124, 715, 1507, 2010 |
| Esp3I | CGTCTC | 1 cut(s) | 2009 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1500, 1532, 2258, 2290 |
| FaqI | GGGAC | 1 cut(s) | 96 |
| FauI | CCCGC | 2 cut(s) | 366, 1783 |
| FauNDI | CATATG | 3 cut(s) | 58, 187, 511 |
| FbaI | TGATCA | 4 cut(s) | 1024, 1384, 1435, 2226 |
| FokI | GGATG | 2 cut(s) | 866, 1729 |
| FriOI | GRGCYC | 1 cut(s) | 625 |
| FspBI | CTAG | 3 cut(s) | 524, 716, 1320 |
| FspI | TGCGCA | 1 cut(s) | 876 |
| GlaI | GCGC | 3 cut(s) | 876, 2219, 2412 |
| GsuI | CTGGAG | 1 cut(s) | 525 |
| HaeII | RGCGCY | 2 cut(s) | 2221, 2414 |
| HaeIII | GGCC | 7 cut(s) | 29, 786, 970, 1145, 1217, 2419, 2676 |
| HapII | CCGG | 2 cut(s) | 199, 1488 |
| HgaI | GACGC | 3 cut(s) | 107, 1624, 2026 |
| HhaI | GCGC | 3 cut(s) | 877, 2220, 2413 |
| Hin1I | GRCGYC | 1 cut(s) | 99 |
| Hin6I | GCGC | 3 cut(s) | 875, 2218, 2411 |
| HinP1I | GCGC | 3 cut(s) | 875, 2218, 2411 |
| HincII | GTYRAC | 3 cut(s) | 153, 2382, 2458 |
| HindII | GTYRAC | 3 cut(s) | 153, 2382, 2458 |
| HindIII | AAGCTT | 1 cut(s) | 704 |
| HpaI | GTTAAC | 1 cut(s) | 153 |
| HpaII | CCGG | 2 cut(s) | 199, 1488 |
| Hpy166II | GTNNAC | 5 cut(s) | 153, 1422, 2382, 2433, 2458 |
| Hpy8I | GTNNAC | 5 cut(s) | 153, 1422, 2382, 2433, 2458 |
| HpyCH4III | ACNGT | 8 cut(s) | 887, 1077, 1237, 1361, 1419, 2455, 2612, 2683 |
| HpyCH4IV | ACGT | 1 cut(s) | 2515 |
| HpySE526I | ACGT | 1 cut(s) | 2515 |
| Hsp92I | GRCGYC | 1 cut(s) | 99 |
| HspAI | GCGC | 3 cut(s) | 875, 2218, 2411 |
| Kpn2I | TCCGGA | 1 cut(s) | 198 |
| Ksp22I | TGATCA | 4 cut(s) | 1024, 1384, 1435, 2226 |
| KspAI | GTTAAC | 1 cut(s) | 153 |
| LmnI | GCTCC | 4 cut(s) | 5, 2132, 2173, 2623 |
| LweI | GCATC | 3 cut(s) | 561, 844, 1694 |
| MaeI | CTAG | 3 cut(s) | 524, 716, 1320 |
| MaeII | ACGT | 1 cut(s) | 2515 |
| MaeIII | GTNAC | 5 cut(s) | 351, 710, 932, 1355, 2483 |
| MfeI | CAATTG | 1 cut(s) | 2234 |
| MflI | RGATCY | 5 cut(s) | 319, 1006, 1120, 1330, 1951 |
| MhlI | GDGCHC | 3 cut(s) | 625, 1227, 2400 |
| MlyI | GAGTC | 5 cut(s) | 656, 1270, 2015, 2111, 2387 |
| MmeI | TCCRAC | 2 cut(s) | 1050, 1256 |
| Mph1103I | ATGCAT | 2 cut(s) | 1672, 2304 |
| MroI | TCCGGA | 1 cut(s) | 198 |
| MroXI | GAANNNNTTC | 2 cut(s) | 339, 2475 |
| MslI | CAYNNNNRTG | 3 cut(s) | 246, 1281, 1665 |
| MspA1I | CMGCKG | 1 cut(s) | 1688 |
| MspCI | CTTAAG | 1 cut(s) | 1603 |
| MspI | CCGG | 2 cut(s) | 199, 1488 |
| MspR9I | CCNGG | 2 cut(s) | 1032, 1104 |
| MunI | CAATTG | 1 cut(s) | 2234 |
| Mva1269I | GAATGC | 3 cut(s) | 823, 1660, 2666 |
| MvaI | CCWGG | 2 cut(s) | 1032, 1104 |
| NcoI | CCATGG | 1 cut(s) | 124 |
| NdeI | CATATG | 3 cut(s) | 58, 187, 511 |
| NlaIV | GGNNCC | 5 cut(s) | 7, 1122, 1392, 1953, 2397 |
| NmuCI | GTSAC | 5 cut(s) | 351, 710, 932, 1355, 2483 |
| NsbI | TGCGCA | 1 cut(s) | 876 |
| NsiI | ATGCAT | 2 cut(s) | 1672, 2304 |
| NspI | RCATGY | 4 cut(s) | 245, 1280, 1471, 1670 |
| PaeI | GCATGC | 1 cut(s) | 1280 |
| PagI | TCATGA | 2 cut(s) | 343, 1453 |
| PasI | CCCWGGG | 1 cut(s) | 1103 |
| PceI | AGGCCT | 3 cut(s) | 786, 970, 2676 |
| PctI | GAATGC | 3 cut(s) | 823, 1660, 2666 |
| PdmI | GAANNNNTTC | 2 cut(s) | 339, 2475 |
| PfeI | GAWTC | 6 cut(s) | 757, 1724, 1859, 2291, 2339, 2636 |
| Pfl23II | CGTACG | 1 cut(s) | 2612 |
| PflFI | GACNNNGTC | 1 cut(s) | 1179 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 125, 2152 |
| PleI | GAGTC | 5 cut(s) | 655, 1269, 2014, 2111, 2386 |
| PpsI | GAGTC | 5 cut(s) | 655, 1269, 2014, 2111, 2386 |
| PshBI | ATTAAT | 1 cut(s) | 2369 |
| PsiI | TTATAA | 1 cut(s) | 675 |
| Psp124BI | GAGCTC | 1 cut(s) | 625 |
| Psp1406I | AACGTT | 1 cut(s) | 2515 |
| Psp6I | CCWGG | 2 cut(s) | 1030, 1102 |
| PspEI | GGTNACC | 2 cut(s) | 710, 2483 |
| PspGI | CCWGG | 2 cut(s) | 1030, 1102 |
| PspLI | CGTACG | 1 cut(s) | 2612 |
| PspN4I | GGNNCC | 5 cut(s) | 7, 1122, 1392, 1953, 2397 |
| PspPI | GGNCC | 4 cut(s) | 169, 1143, 2417, 2683 |
| PstI | CTGCAG | 2 cut(s) | 955, 1380 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2092 |
| PsuI | RGATCY | 5 cut(s) | 319, 1006, 1120, 1330, 1951 |
| PsyI | GACNNNGTC | 1 cut(s) | 1179 |
| RsaI | GTAC | 7 cut(s) | 863, 1416, 1867, 1976, 2528, 2574, 2614 |
| RsaNI | GTAC | 7 cut(s) | 862, 1415, 1866, 1975, 2527, 2573, 2613 |
| RseI | CAYNNNNRTG | 3 cut(s) | 246, 1281, 1665 |
| SacI | GAGCTC | 1 cut(s) | 625 |
| Sau96I | GGNCC | 4 cut(s) | 169, 1143, 2417, 2683 |
| ScaI | AGTACT | 2 cut(s) | 1976, 2528 |
| SchI | GAGTC | 5 cut(s) | 656, 1270, 2015, 2111, 2387 |
| ScrFI | CCNGG | 2 cut(s) | 1032, 1104 |
| SduI | GDGCHC | 3 cut(s) | 625, 1227, 2400 |
| SfaNI | GCATC | 3 cut(s) | 561, 844, 1694 |
| SfcI | CTRYAG | 4 cut(s) | 298, 359, 951, 1376 |
| SinI | GGWCC | 2 cut(s) | 169, 2683 |
| SmiI | ATTTAAAT | 1 cut(s) | 471 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 246, 1281, 1665 |
| SmlI | CTYRAG | 3 cut(s) | 626, 1064, 1603 |
| SmoI | CTYRAG | 3 cut(s) | 626, 1064, 1603 |
| SphI | GCATGC | 1 cut(s) | 1280 |
| SseBI | AGGCCT | 3 cut(s) | 786, 970, 2676 |
| SsiI | CCGC | 8 cut(s) | 172, 373, 1504, 1688, 1776, 1922, 2312, 2492 |
| SspI | AATATT | 1 cut(s) | 331 |
| SspMI | CTAG | 3 cut(s) | 524, 716, 1320 |
| SstI | GAGCTC | 1 cut(s) | 625 |
| StuI | AGGCCT | 3 cut(s) | 786, 970, 2676 |
| StyD4I | CCNGG | 2 cut(s) | 1030, 1102 |
| StyI | CCWWGG | 4 cut(s) | 124, 715, 1507, 2010 |
| SwaI | ATTTAAAT | 1 cut(s) | 471 |
| TaaI | ACNGT | 8 cut(s) | 887, 1077, 1237, 1361, 1419, 2455, 2612, 2683 |
| TaiI | ACGT | 1 cut(s) | 2518 |
| TaqI | TCGA | 8 cut(s) | 772, 943, 1096, 1130, 1202, 1632, 1654, 1911 |
| TaqII | GACCGA | 1 cut(s) | 252 |
| TatI | WGTACW | 5 cut(s) | 861, 1414, 1865, 1974, 2526 |
| TauI | GCSGC | 1 cut(s) | 2495 |
| TfiI | GAWTC | 6 cut(s) | 757, 1724, 1859, 2291, 2339, 2636 |
| TscAI | CASTG | 2 cut(s) | 1311, 1469 |
| TseFI | GTSAC | 5 cut(s) | 351, 710, 932, 1355, 2483 |
| Tsp45I | GTSAC | 5 cut(s) | 351, 710, 932, 1355, 2483 |
| TspGWI | ACGGA | 2 cut(s) | 2613, 2630 |
| TspRI | CASTG | 2 cut(s) | 1311, 1469 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1179 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 125, 2152 |
| Vha464I | CTTAAG | 1 cut(s) | 1603 |
| VpaK11BI | GGWCC | 2 cut(s) | 169, 2683 |
| VspI | ATTAAT | 1 cut(s) | 2369 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 980 |
| XapI | RAATTY | 7 cut(s) | 472, 498, 1535, 1643, 2138, 2269, 2656 |
| XbaI | TCTAGA | 1 cut(s) | 523 |
| XceI | RCATGY | 4 cut(s) | 245, 1280, 1471, 1670 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1514 |
| XmaJI | CCTAGG | 1 cut(s) | 715 |
| XmnI | GAANNNNTTC | 2 cut(s) | 339, 2475 |
| XspI | CTAG | 3 cut(s) | 524, 716, 1320 |
| ZrmI | AGTACT | 2 cut(s) | 1976, 2528 |
| Zsp2I | ATGCAT | 2 cut(s) | 1672, 2304 |