pycom17g21690

Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0014585
Physical Location & Seq
Reverse (-)
20153266 .. 20155263
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
ATGGGGAGAAAGTGGATTCCAATGGACGTGGCCACAGCTGGTGTGGTGGCGGCAATGCATTTGCTTAGCCTTTTTGCACCTTTTCAATTCAATTGGGGAGCTTTTTGGGTGGCAGTGGCACTTTATGTTGTGACTGGTCTATTTGGAATCACTCTTTCTTTTCATAGGAATCTCTCGCACAAGAGTTTCAAGCTCCCCAGGTTTCTTGAGTACTTATTTGCTTATTGTGGATCCCAAGCACTTCAGGGGAGCCCAATTGATTGGGTGAGTGTGCACAGGTACCACCACCAGTTCTGTGATTCTGAGAAAGACCCACATAGCCCAATTGAAGGTTTCTGGTACAGTCACATGAGTTGGCTCTTTGATACCGAATCTGTGAATGAAAGGTGTGGGGGATCAAATAATGCTGGGGATCTAGAGAAGCAGCCCTTCTATCAGCTCCTGCATAAAACTTACATTGCTCACCCTATTGCTCTTGGACTTGTACTATATGCAATGGGAGGATTCCCATTCCTTGTGTGGGGAATGGGTGTGAGGACTATATGGGTATACCACATTACTTGGCTTGTTAACTCAGCTTGCCACGTTTGGGGAAACAGAGCATGGAATACCGGTGACTTGTCTAGGAACAATTGGTGGGTGGCAATACTAGCGTTTGGAGAGGGTTGGCACAACAATCACCATGCATTTGAGTACTCGGCTAGGCATGGCCTGAAATGGTGGCAGCTTGACATGACATGGTATGTTGTAAGATTTCTCCAAGCTATTGGATTGGCAACTGATGTGAAGCTACCAACTGAGGCCCACAAACAAAGGATGGCTTTGAACTAA

Protein Analysis

277

Amino Acids

31.85

Weight (kDa)

8.33

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 35 - 251 1.2e-17 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014585)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15850
fragaria_vesca FvH4_6g29730
malus_domestica MD17G1212000.v1.1
prunus_persica Prupe.3G087200_v2.0.a1 Prupe.3G087200_v2.0.a1
pyrus_communis pycom17g21690
rosa_chinensis RchiOBHm_Chr2g0135421
rosa_laevigata RLG00000019462
rosa_multiflora Rmu_ssc0000187.1_g000002
rosa_roxburghii Rroxscaffold_2G00109260
rosa_rugosa Rorug02G0324700
rosa_samantha Rh2AG370200 Rh2BG381900 Rh2CG360500 Rh2CG360600 Rh2DG398200
rosa_wichuraiana Rw2G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 279
AccI GTMKAC 1 cut(s) 549
AciI CCGC 1 cut(s) 50
AclWI GGATC 4 cut(s) 225, 238, 403, 420
AcoI YGGCCR 1 cut(s) 30
AcuI CTGAAG 1 cut(s) 227
AfaI GTAC 5 cut(s) 212, 281, 341, 486, 695
AfiI CCNNNNNNNGG 3 cut(s) 329, 520, 589
AgeI ACCGGT 1 cut(s) 611
AgsI TTSAA 5 cut(s) 86, 91, 190, 329, 826
AjiI CACGTC 1 cut(s) 28
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 8 cut(s) 38, 101, 193, 439, 578, 727, 764, 790
AluI AGCT 8 cut(s) 38, 101, 193, 439, 578, 727, 764, 790
Alw21I GWGCWC 1 cut(s) 276
Alw44I GTGCAC 1 cut(s) 272
AlwI GGATC 4 cut(s) 225, 238, 403, 420
AlwNI CAGNNNCTG 1 cut(s) 442
AoxI GGCC 3 cut(s) 30, 709, 801
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 424, 724
AsiGI ACCGGT 1 cut(s) 611
Asp718I GGTACC 1 cut(s) 279
AspS9I GGNCC 1 cut(s) 802
AsuHPI GGTGA 4 cut(s) 277, 455, 626, 671
BaeGI GKGCMC 1 cut(s) 276
BalI TGGCCA 1 cut(s) 32
BamHI GGATCC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 279
BanII GRGCYC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 2 cut(s) 436, 736
BccI CCATC 1 cut(s) 811
BciT130I CCWGG 1 cut(s) 199
BfaI CTAG 4 cut(s) 416, 624, 650, 702
BisI GCNGC 3 cut(s) 51, 425, 725
BlpI GCTNAGC 1 cut(s) 65
BlsI GCNGC 3 cut(s) 52, 426, 726
BmcAI AGTACT 2 cut(s) 212, 695
Bme1390I CCNGG 1 cut(s) 199
BmgBI CACGTC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 802
BmiI GGNNCC 3 cut(s) 232, 251, 281
BmrFI CCNGG 1 cut(s) 199
Bpu1102I GCTNAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 197
BsaWI WCCGGW 1 cut(s) 611
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 520, 589
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 2 cut(s) 139, 289
Bse3DI GCAATG 3 cut(s) 60, 456, 501
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 197
BseGI GGATG 1 cut(s) 822
BseLI CCNNNNNNNGG 3 cut(s) 329, 520, 589
BseMI GCAATG 3 cut(s) 60, 456, 501
BseMII CTCAG 3 cut(s) 294, 588, 789
BseNI ACTGG 2 cut(s) 139, 289
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 436, 736
BseYI CCCAGC 1 cut(s) 407
BshFI GGCC 3 cut(s) 32, 711, 803
BshNI GGYRCC 1 cut(s) 279
BshTI ACCGGT 1 cut(s) 611
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 1 cut(s) 612
BslI CCNNNNNNNGG 3 cut(s) 329, 520, 589
BsnI GGCC 3 cut(s) 32, 711, 803
Bsp1286I GDGCHC 2 cut(s) 254, 276
Bsp143I GATC 3 cut(s) 230, 395, 412
Bsp1720I GCTNAGC 1 cut(s) 65
BspACI CCGC 1 cut(s) 50
BspANI GGCC 3 cut(s) 32, 711, 803
BspCNI CTCAG 3 cut(s) 295, 587, 790
BspLI GGNNCC 3 cut(s) 232, 251, 281
BspPI GGATC 4 cut(s) 225, 238, 403, 420
BspT107I GGYRCC 1 cut(s) 279
BsrDI GCAATG 3 cut(s) 60, 456, 501
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 2 cut(s) 139, 289
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 1 cut(s) 197
BssMI GATC 3 cut(s) 230, 395, 412
BssNAI GTATAC 1 cut(s) 550
Bst1107I GTATAC 1 cut(s) 550
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 580
BstDEI CTNAG 4 cut(s) 65, 303, 574, 798
BstF5I GGATG 1 cut(s) 822
BstKTI GATC 3 cut(s) 233, 398, 415
BstMBI GATC 3 cut(s) 230, 395, 412
BstMWI GCNNNNNNNGC 1 cut(s) 650
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 2 cut(s) 436, 736
BstX2I RGATCY 2 cut(s) 230, 412
BstXI CCANNNNNNTGG 2 cut(s) 261, 767
BstYI RGATCY 2 cut(s) 230, 412
BstZ17I GTATAC 1 cut(s) 550
BsuRI GGCC 3 cut(s) 32, 711, 803
BtrI CACGTC 1 cut(s) 28
BtsCI GGATG 1 cut(s) 822
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 580
CaiI CAGNNNCTG 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 1 cut(s) 802
Csp6I GTAC 5 cut(s) 211, 280, 340, 485, 694
CspAI ACCGGT 1 cut(s) 611
CspCI CAANNNNNGTGG 4 cut(s) 9, 44, 542, 577
CviAII CATG 6 cut(s) 349, 603, 683, 707, 733, 738
CviQI GTAC 5 cut(s) 211, 280, 340, 485, 694
DdeI CTNAG 4 cut(s) 65, 303, 574, 798
DpnI GATC 3 cut(s) 232, 397, 414
DpnII GATC 3 cut(s) 230, 395, 412
EaeI YGGCCR 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 197
EcoT22I ATGCAT 2 cut(s) 60, 688
EcoT38I GRGCYC 1 cut(s) 254
FaeI CATG 6 cut(s) 352, 606, 686, 710, 736, 741
FalI AAGNNNNNCTT 3 cut(s) 413, 445, 805
FatI CATG 6 cut(s) 348, 602, 682, 706, 732, 737
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 3 cut(s) 51, 425, 725
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 3 cut(s) 51, 425, 725
FspBI CTAG 4 cut(s) 416, 624, 650, 702
GluI GCNGC 3 cut(s) 51, 425, 725
GsaI CCCAGC 1 cut(s) 411
HaeIII GGCC 3 cut(s) 32, 711, 803
HapII CCGG 1 cut(s) 612
Hin1II CATG 6 cut(s) 352, 606, 686, 710, 736, 741
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 6 cut(s) 16, 147, 169, 299, 371, 504
HpaI GTTAAC 1 cut(s) 571
HpaII CCGG 1 cut(s) 612
HphI GGTGA 4 cut(s) 277, 455, 626, 671
Hpy166II GTNNAC 3 cut(s) 274, 550, 571
Hpy188I TCNGA 1 cut(s) 304
Hpy188III TCNNGA 2 cut(s) 206, 416
Hpy8I GTNNAC 3 cut(s) 274, 550, 571
HpyAV CCTTC 2 cut(s) 323, 439
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4IV ACGT 2 cut(s) 27, 585
HpyCH4V TGCA 6 cut(s) 58, 77, 274, 445, 494, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 650
HpyF3I CTNAG 4 cut(s) 65, 303, 574, 798
HpySE526I ACGT 2 cut(s) 27, 585
Hsp92II CATG 6 cut(s) 352, 606, 686, 710, 736, 741
KpnI GGTACC 1 cut(s) 283
KspAI GTTAAC 1 cut(s) 571
Kzo9I GATC 3 cut(s) 230, 395, 412
LmnI GCTCC 4 cut(s) 98, 198, 249, 444
Lsp1109I GCAGC 2 cut(s) 436, 736
MaeI CTAG 4 cut(s) 416, 624, 650, 702
MaeII ACGT 2 cut(s) 27, 585
MaeIII GTNAC 3 cut(s) 130, 344, 614
MalI GATC 3 cut(s) 232, 397, 414
MboI GATC 3 cut(s) 230, 395, 412
MfeI CAATTG 4 cut(s) 91, 255, 324, 631
MflI RGATCY 2 cut(s) 230, 412
MhlI GDGCHC 2 cut(s) 254, 276
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 5 cut(s) 86, 91, 255, 324, 631
MluNI TGGCCA 1 cut(s) 32
MnlI CCTC 4 cut(s) 494, 528, 655, 793
Mox20I TGGCCA 1 cut(s) 32
Mph1103I ATGCAT 2 cut(s) 60, 688
MscI TGGCCA 1 cut(s) 32
MseI TTAA 1 cut(s) 570
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 1 cut(s) 612
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 4 cut(s) 91, 255, 324, 631
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 1 cut(s) 650
NdeII GATC 3 cut(s) 230, 395, 412
NlaIII CATG 6 cut(s) 352, 606, 686, 710, 736, 741
NlaIV GGNNCC 3 cut(s) 232, 251, 281
NmeAIII GCCGAG 1 cut(s) 677
NmuCI GTSAC 3 cut(s) 130, 344, 614
NsiI ATGCAT 2 cut(s) 60, 688
PfeI GAWTC 6 cut(s) 16, 147, 169, 299, 371, 504
PinAI ACCGGT 1 cut(s) 611
PkrI GCNGC 3 cut(s) 52, 426, 726
Psp6I CCWGG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 3 cut(s) 232, 251, 281
PspPI GGNCC 1 cut(s) 802
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 2 cut(s) 230, 412
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 5 cut(s) 212, 281, 341, 486, 695
RsaNI GTAC 5 cut(s) 211, 280, 340, 485, 694
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 3 cut(s) 51, 425, 725
Sau3AI GATC 3 cut(s) 230, 395, 412
Sau96I GGNCC 1 cut(s) 802
ScaI AGTACT 2 cut(s) 212, 695
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 2 cut(s) 254, 276
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 5 cut(s) 86, 91, 255, 324, 631
SsiI CCGC 1 cut(s) 50
SspMI CTAG 4 cut(s) 416, 624, 650, 702
StyD4I CCNGG 1 cut(s) 197
TaaI ACNGT 1 cut(s) 344
TaiI ACGT 2 cut(s) 30, 588
TasI AATT 5 cut(s) 86, 91, 255, 324, 631
TatI WGTACW 3 cut(s) 210, 484, 693
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 6 cut(s) 16, 147, 169, 299, 371, 504
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 3 cut(s) 130, 344, 614
TseI GCWGC 2 cut(s) 424, 724
Tsp45I GTSAC 3 cut(s) 130, 344, 614
TspDTI ATGAA 2 cut(s) 152, 396
TspRI CASTG 1 cut(s) 120
VneI GTGCAC 1 cut(s) 272
XbaI TCTAGA 1 cut(s) 415
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 516
XmiI GTMKAC 1 cut(s) 549
XspI CTAG 4 cut(s) 416, 624, 650, 702
ZrmI AGTACT 2 cut(s) 212, 695
Zsp2I ATGCAT 2 cut(s) 60, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.