pycom17g22520

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0016889
Physical Location & Seq
Reverse (-)
20915183 .. 20915987
805 bp
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UTR
Exon/CDS
Intron
pycom17g22520.1

Sequence Viewer

Length: 588 bp
ATGGCTAGATCCCAGCAGCGGTATCGAGGCGTCCGACAGAGGCATTGGGGCTCTTGGGTCTCCGAGATTCGCCACCCTCTACTGAAGACACGAATTTGGCTAGGCACATTCGAAACTGCGGAGGACGCTGCACGAGCATACGATGAAGCAGCAAGGCTCATGTGTGGCCTAAAAGCGCGCACAAATTTCCCATACAATCCTAACGAGCCCATGTTGCATTCATCCACGCTTCTCTCAGCAGCTTTGATGGCAAAGCTGAACAAATGCAACATGGCCTCTCTCCAAATGGCCAAAACTACCAAACCTGCAGTGCCAAAGGAGGAGCCCCAAGAACAAGTACACATGCCACTCCCTTCTACCACCTGGATTACCGGAAAAGGGGGCGAGAAGGTCAGGGATTATAGCGGAGCAGAACAATGGGTAGATCATGGGAACTGGGTTAGTGTTGATCTGCAGGGGGTTCCTCATGATCATCATCATCAGCAGCAGGAGTTTATTAAGCCTCTTCAGGATGATCATATTGAGCAGATGATTGAGGAGCTTCTTGATTACGGCTACATTGAGATTTGTCCTGGTGTTTCTGCATAG

Protein Analysis

196

Amino Acids

22.27

Weight (kDa)

6.36

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 7 - 56 2.2e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016889)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28880
malus_domestica MD17G1220600.v1.1
prunus_persica Prupe.3G096000_v2.0.a1
pyrus_communis pycom17g22520
rosa_chinensis RchiOBHm_Chr2g0133451
rosa_laevigata RLG00000019335
rosa_multiflora Rmu_sc0006007.1_g000007
rosa_roxburghii Rroxscaffold_2G00111120
rosa_rugosa Rorug02G0311800
rosa_samantha Rh2AG364400 Rh2BG370100 Rh2CG347600 Rh2DG387000
rosa_wichuraiana Rw2G029680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccII CGCG 1 cut(s) 178
AciI CCGC 3 cut(s) 19, 119, 405
AclWI GGATC 1 cut(s) 3
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 2 cut(s) 93, 184
AcuI CTGAAG 2 cut(s) 104, 491
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 2 cut(s) 18, 378
AjnI CCWGG 2 cut(s) 362, 571
AluBI AGCT 3 cut(s) 242, 256, 541
AluI AGCT 3 cut(s) 242, 256, 541
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 1 cut(s) 3
AoxI GGCC 3 cut(s) 166, 273, 288
ApeKI GCWGC 5 cut(s) 16, 128, 149, 239, 484
ApoI RAATTY 2 cut(s) 93, 184
AspLEI GCGC 2 cut(s) 178, 180
AsuII TTCGAA 1 cut(s) 111
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 3 cut(s) 53, 210, 327
BauI CACGAG 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 92
BbvI GCAGC 5 cut(s) 28, 115, 161, 251, 496
BccI CCATC 1 cut(s) 241
BceAI ACGGC 1 cut(s) 568
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 364, 573
BclI TGATCA 2 cut(s) 469, 514
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 2 cut(s) 6, 101
BfmI CTRYAG 2 cut(s) 306, 452
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 5 cut(s) 17, 129, 150, 240, 485
BlsI GCNGC 5 cut(s) 18, 130, 151, 241, 486
Bme1390I CCNGG 2 cut(s) 364, 573
BmiI GGNNCC 2 cut(s) 324, 462
BmrFI CCNGG 2 cut(s) 364, 573
BmrI ACTGGG 1 cut(s) 445
BmuI ACTGGG 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 92
Bpu14I TTCGAA 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 64
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 378
Bse1I ACTGG 1 cut(s) 440
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 2 cut(s) 364, 573
BseGI GGATG 2 cut(s) 221, 517
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 18, 378
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 440
BsePI GCGCGC 1 cut(s) 176
BseRI GAGGAG 2 cut(s) 335, 551
BseXI GCAGC 5 cut(s) 28, 115, 161, 251, 496
BseYI CCCAGC 1 cut(s) 12
BsgI GTGCAG 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 3 cut(s) 168, 275, 290
BsiSI CCGG 1 cut(s) 372
BslI CCNNNNNNNGG 2 cut(s) 18, 378
BsmAI GTCTC 1 cut(s) 64
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 3 cut(s) 168, 275, 290
Bso31I GGTCTC 1 cut(s) 64
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 53, 210, 327
Bsp143I GATC 5 cut(s) 8, 424, 448, 469, 514
BspACI CCGC 3 cut(s) 19, 119, 405
BspANI GGCC 3 cut(s) 168, 275, 290
BspCNI CTCAG 1 cut(s) 248
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 324, 462
BspMAI CTGCAG 2 cut(s) 310, 456
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 1 cut(s) 3
BspT104I TTCGAA 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 64
BsrI ACTGG 1 cut(s) 440
BssHII GCGCGC 1 cut(s) 176
BssMI GATC 5 cut(s) 8, 424, 448, 469, 514
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 132
Bst2UI CCWGG 2 cut(s) 364, 573
Bst6I CTCTTC 1 cut(s) 510
BstACI GRCGYC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 1 cut(s) 235
BstF5I GGATG 2 cut(s) 221, 517
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 2 cut(s) 178, 180
BstKTI GATC 5 cut(s) 11, 427, 451, 472, 517
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 5 cut(s) 8, 424, 448, 469, 514
BstMWI GCNNNNNNNGC 4 cut(s) 125, 134, 214, 248
BstNI CCWGG 2 cut(s) 364, 573
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 362, 571
BstSFI CTRYAG 2 cut(s) 306, 452
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 5 cut(s) 28, 115, 161, 251, 496
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 3 cut(s) 168, 275, 290
BtsCI GGATG 2 cut(s) 221, 517
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 315
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 1 cut(s) 178
CciI TCATGA 1 cut(s) 466
CfoI GCGC 2 cut(s) 178, 180
CseI GACGC 2 cut(s) 19, 134
Csp6I GTAC 1 cut(s) 338
CviAII CATG 6 cut(s) 160, 211, 271, 343, 428, 467
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 1 cut(s) 235
DpnI GATC 5 cut(s) 10, 426, 450, 471, 516
DpnII GATC 5 cut(s) 8, 424, 448, 469, 514
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco24I GRGCYC 3 cut(s) 53, 210, 327
Eco31I GGTCTC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 104, 491
EcoRII CCWGG 2 cut(s) 362, 571
EcoT38I GRGCYC 3 cut(s) 53, 210, 327
FaeI CATG 6 cut(s) 163, 214, 274, 346, 431, 470
FatI CATG 6 cut(s) 159, 210, 270, 342, 427, 466
FbaI TGATCA 2 cut(s) 469, 514
Fnu4HI GCNGC 5 cut(s) 17, 129, 150, 240, 485
FokI GGATG 2 cut(s) 208, 524
FriOI GRGCYC 3 cut(s) 53, 210, 327
Fsp4HI GCNGC 5 cut(s) 17, 129, 150, 240, 485
FspBI CTAG 2 cut(s) 6, 101
GlaI GCGC 2 cut(s) 177, 179
GluI GCNGC 5 cut(s) 17, 129, 150, 240, 485
GsaI CCCAGC 1 cut(s) 16
HaeIII GGCC 3 cut(s) 168, 275, 290
HapII CCGG 1 cut(s) 372
HgaI GACGC 2 cut(s) 19, 134
HhaI GCGC 2 cut(s) 178, 180
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 6 cut(s) 163, 214, 274, 346, 431, 470
Hin6I GCGC 2 cut(s) 176, 178
HinP1I GCGC 2 cut(s) 176, 178
HinfI GANTC 1 cut(s) 67
HpaII CCGG 1 cut(s) 372
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 35, 64
Hpy188III TCNNGA 3 cut(s) 467, 509, 545
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 2 cut(s) 363, 382
HpyCH4V TGCA 6 cut(s) 131, 217, 267, 308, 454, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 134, 214, 248
HpyF3I CTNAG 1 cut(s) 235
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 6 cut(s) 163, 214, 274, 346, 431, 470
HspAI GCGC 2 cut(s) 176, 178
Ksp22I TGATCA 2 cut(s) 469, 514
Kzo9I GATC 5 cut(s) 8, 424, 448, 469, 514
LmnI GCTCC 3 cut(s) 322, 407, 538
Lsp1109I GCAGC 5 cut(s) 28, 115, 161, 251, 496
MaeI CTAG 2 cut(s) 6, 101
MalI GATC 5 cut(s) 10, 426, 450, 471, 516
MboI GATC 5 cut(s) 8, 424, 448, 469, 514
MboII GAAGA 2 cut(s) 97, 497
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 3 cut(s) 53, 210, 327
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 2 cut(s) 93, 184
MluNI TGGCCA 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 9 cut(s) 20, 33, 87, 115, 286, 313, 474, 513, 529
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 1 cut(s) 498
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 364, 573
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 2 cut(s) 364, 573
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 4 cut(s) 125, 134, 214, 248
NdeII GATC 5 cut(s) 8, 424, 448, 469, 514
NlaIII CATG 6 cut(s) 163, 214, 274, 346, 431, 470
NlaIV GGNNCC 2 cut(s) 324, 462
NspI RCATGY 1 cut(s) 346
NspV TTCGAA 1 cut(s) 111
PagI TCATGA 1 cut(s) 466
PauI GCGCGC 1 cut(s) 176
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PctI GAATGC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 5 cut(s) 18, 130, 151, 241, 486
Psp6I CCWGG 2 cut(s) 362, 571
PspFI CCCAGC 1 cut(s) 12
PspGI CCWGG 2 cut(s) 362, 571
PspN4I GGNNCC 2 cut(s) 324, 462
PstI CTGCAG 2 cut(s) 310, 456
PsuI RGATCY 1 cut(s) 8
PteI GCGCGC 1 cut(s) 176
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 5 cut(s) 17, 129, 150, 240, 485
Sau3AI GATC 5 cut(s) 8, 424, 448, 469, 514
ScrFI CCNGG 2 cut(s) 364, 573
SduI GDGCHC 3 cut(s) 53, 210, 327
SetI ASST 6 cut(s) 244, 258, 307, 365, 393, 543
SfcI CTRYAG 2 cut(s) 306, 452
SfuI TTCGAA 1 cut(s) 111
Sse9I AATT 2 cut(s) 93, 184
SsiI CCGC 3 cut(s) 19, 119, 405
SspMI CTAG 2 cut(s) 6, 101
StyD4I CCNGG 2 cut(s) 362, 571
TaqI TCGA 2 cut(s) 25, 111
TasI AATT 2 cut(s) 93, 184
TatI WGTACW 1 cut(s) 337
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 1 cut(s) 315
TseI GCWGC 5 cut(s) 16, 128, 149, 239, 484
TspDTI ATGAA 2 cut(s) 159, 210
TspRI CASTG 1 cut(s) 315
XapI RAATTY 2 cut(s) 93, 184
XceI RCATGY 1 cut(s) 346
XspI CTAG 2 cut(s) 6, 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.