pycom17g23730

GTP-binding protein TypA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005874
Physical Location & Seq
Forward (+)
22010095 .. 22019177
9083 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2241 bp
ATGGTGGTCGGGCAAGGTCCCCGGACTTCCATGCAGTTTTTCTTTTGGGTAGCTGTTCACTGTTGGATCTCGCCATACATCACCGTTGGACTTCCAGTTCGAACTTTCACCGTGGTGGAACATCATGGCCCATGTAAAATCTGGTTTAGTCTTAAAGCAACGTTAAACCTTAAACCCTGGCAATTGCACGGGAACTACTCAGCCATTTCTGTCTCGCTGTTAGAAATGGCGGGGCCTTTGCTTCGCTCTCTCTGGTCTTCCACGCGCAACTCCTTCTCTTCATCCTCCGCGTCCACCACTCTCAAACCCTATGCATTAACCCCACGCGCATCTCCACTCGCACGCGCCTTCTCGGCAGCCACGGCGGCCTCCAGTGCCACTTCACCGAACAGCTCCCTGGATCCGAGCCGCCTCCGCAACGTGGCGGTAATAGCCCATGTCGACCACGGCAAGACGACGCTCATGGACCGCCTGCTCCGCCAGTGCGGCGCCGACATACCCCACGAACGAGCCATGGATTCCATCAGCCTCGAGCGCGAGCGCGGGATTACTATCGCCTCCAAGGTTACATCAATTCCGTGGAAGGAGAATGAGTTAAACATGGTTGATACGCCTGGACATGCCGATTTTGGTGGTGAAGTGGAGCGGGTTGTTGGTATGGTTGAGGGGGCGATTTTAGTTGTTGATGCCGGAGAAGGTCCGCTTGCACAGACCAAGTTTGTACTTGCTAAAGCCTTAAAATACGGGCTGCGCCCTATTCTTCTTCTAAACAAAGTAGATCGTCCTGCAGTTTCTGAGGAGATGTGTAATGAAGTTGAGAGTTTGGTATTTGATCTTTTTGCGAATCTTGGTGCTACAGAGGAGCAGCTAGATTTTCCTGTGCTATATGCTTCTGCTAAAGAAGGATGGGCTTCGACCACTTTCACCAAAGATCCTCCTGCTGATGCAAGAAACATGTCACGGCTGCTTGATGCCATTATAAGTCATGTTCCTCCACCAACAGCCAATCTCAATGCACCTTTTCAAATGCTGGTTTCTATGATGGAACGAGACTTCTACCTTGGACGAATATTGACCGGACGAGTTTCTTCTGGCAGCATTCGCATTGGTGACAGAGTTCATGGGCTTCGTCACAAAGATTCTGGAGTTGAGAAAATTGAAGAAGGGAAGGTTGTGAAGCTGATGAAAAAGAAGGGAACACATATGGTCGTAATTGAATGTGCTGGAGCTGGTGACATAGTGTCAATGGCGGGTTTAACAAGCCCATCTATTGGCCATACAGTTGCAAGCGTTGAGACTATGGCTGCATTGCCCACCGTTGTGTTGGATCCACCAACTATTTCCATGACTTTTGGTGTCAATGACTCTCCCTTAGCTGGTCGTGACGGTACCCATCTCACTGGAGGAAAAATTGGCGATCGACTATCAGCTGAAGCAGAAACAAATCTTGCCATTAATGTTCTCCCAGGCTTATCAGAATCATTTGAAGTTCAGGGGAGGGGAGAACTTCAGCTGGGTATTTTAATTGAGAATATGAGGCGGGAAGGCTTTGAGCTGTCTGTCTCACCGCCCAAAGTCATGTACAAAACAGAGAGTGGTCATAAACTTGAGCCAATTGAAGAAGTTACTATTGAGGTAAATGAAGAGCATGTGGGGTTAGTTATGCAAGCCTTATCTAATAGGCGAGCTGAGGTTGTTGAGATGGGTCCTGTTCCAGGAAGCGCCGACCGGACTAGATTGTCATTGACATGTCCATCAAGGGGCCTGGTTGGTTACAGAAGCGTATTTAGCAGTGACACACGTGGAACTGGATTTATGCACCGTGCTTTCTTAAAATATGAAAAATATCGAGGTCCCCTTGGAGATGTCAGGAAAGGAGTGTTGGTGTCAATGGGCTATGGTACAACCACAGCGTATGCACTAATGAGTTTAGAACCTCGCGGAACTCTTTTTGTCACTCCTGGGATGGAGACATATGATGGCATGATTGTTGGCGAGCACTCGAGGGACACAGATCTAGATGTCAACCCAGTTAGAAATAAGGAACTTACAAATGTGCGTGCTGCTAGCAAGGATGAGAATGTGAAACTATCTCCTCCTCGCCTTATGACTCTTGAAGAAGCAATAGGGTATGTAGCCTCTGATGAACTGATTGAGGTTACACCAAAGACAATACGGTTAAGGAAGAAGTATCTGGATGTTAACAAGCGTAAAAGTATGAGTAAAAGGCAAAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

747

Amino Acids

81.38

Weight (kDa)

7.7

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 137 - 332 6.9e-53 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 340 - 430 3.7e-07 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 355 - 428 6.4e-08 Elongation factor Tu domain 2
EFG_C PF00679 536 - 619 1.4e-19 Elongation factor G C-terminus
BipA_C PF21018 625 - 733 4.8e-45 TypA/BipA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 980
AasI GACNNNNNNGTC 1 cut(s) 1738
Acc65I GGTACC 1 cut(s) 1388
AccB1I GGYRCC 2 cut(s) 488, 1388
AccB7I CCANNNNNTGG 1 cut(s) 1271
AccBSI CCGCTC 1 cut(s) 646
AccI GTMKAC 1 cut(s) 441
AccII CGCG 7 cut(s) 265, 290, 327, 345, 537, 543, 1941
AclI AACGTT 1 cut(s) 161
AclWI GGATC 6 cut(s) 74, 395, 408, 926, 1322, 1335
AcoI YGGCCR 1 cut(s) 1273
AcuI CTGAAG 2 cut(s) 1452, 1493
AcvI CACGTG 1 cut(s) 1802
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 4 cut(s) 723, 1390, 1583, 1903
AfiI CCNNNNNNNGG 5 cut(s) 421, 1271, 1376, 1715, 1760
AflIII ACRYGT 3 cut(s) 954, 1748, 1799
AgsI TTSAA 6 cut(s) 1025, 1160, 1217, 1487, 1619, 2117
AhdI GACNNNNNGTC 1 cut(s) 1240
AjnI CCWGG 7 cut(s) 176, 396, 613, 1465, 1714, 1764, 1960
AjuI GAANNNNNNNTTGG 2 cut(s) 1865, 1897
AleI CACNNNNGTG 2 cut(s) 113, 1319
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
Alw21I GWGCWC 1 cut(s) 2001
Alw26I GTCTC 5 cut(s) 217, 1044, 1289, 1567, 1964
AlwI GGATC 6 cut(s) 74, 395, 408, 926, 1322, 1335
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 2 cut(s) 530, 2002
AoxI GGCC 5 cut(s) 127, 233, 366, 1273, 1762
ApeKI GCWGC 7 cut(s) 356, 748, 865, 964, 1095, 1304, 2063
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AseI ATTAAT 1 cut(s) 1455
Asp718I GGTACC 1 cut(s) 1388
AspLEI GCGC 8 cut(s) 267, 329, 347, 491, 537, 543, 753, 1724
AspS9I GGNCC 8 cut(s) 17, 128, 233, 466, 698, 1706, 1762, 1853
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 8 cut(s) 73, 100, 375, 647, 916, 1121, 1244, 1557
AsuII TTCGAA 1 cut(s) 100
AsuNHI GCTAGC 1 cut(s) 2066
AvaI CYCGRG 2 cut(s) 530, 2002
AvaII GGWCC 5 cut(s) 17, 466, 698, 1706, 1853
BalI TGGCCA 1 cut(s) 1275
BamHI GGATCC 2 cut(s) 400, 1327
BanI GGYRCC 2 cut(s) 488, 1388
BbrPI CACGTG 1 cut(s) 1802
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 2001
BbvCI CCTCAGC 1 cut(s) 1689
BbvI GCAGC 7 cut(s) 368, 735, 877, 951, 1107, 1291, 2050
BccI CCATC 9 cut(s) 530, 900, 1036, 1273, 1401, 1696, 1762, 1960, 1973
BceAI ACGGC 3 cut(s) 378, 463, 977
BciT130I CCWGG 7 cut(s) 178, 398, 615, 1467, 1716, 1766, 1962
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 5 cut(s) 217, 1044, 1289, 1567, 1964
BfaI CTAG 4 cut(s) 869, 1734, 2018, 2067
BfmI CTRYAG 2 cut(s) 786, 855
BfoI RGCGCY 2 cut(s) 492, 1725
BglI GCCNNNNNGGC 3 cut(s) 353, 365, 486
BglII AGATCT 1 cut(s) 2014
Bme1390I CCNGG 8 cut(s) 22, 178, 398, 615, 1467, 1716, 1766, 1962
Bme18I GGWCC 5 cut(s) 17, 466, 698, 1706, 1853
BmeRI GACNNNNNGTC 1 cut(s) 1240
BmeT110I CYCGRG 2 cut(s) 530, 2002
BmgT120I GGNCC 8 cut(s) 17, 128, 233, 466, 698, 1706, 1762, 1853
BmiI GGNNCC 9 cut(s) 19, 234, 402, 490, 1329, 1390, 1707, 1763, 1855
BmrFI CCNGG 8 cut(s) 22, 178, 398, 615, 1467, 1716, 1766, 1962
BmrI ACTGGG 1 cut(s) 2024
BmsI GCATC 4 cut(s) 338, 676, 934, 961
BmtI GCTAGC 1 cut(s) 2070
BmuI ACTGGG 1 cut(s) 2024
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 4 cut(s) 355, 1164, 1245, 1422
Bpu10I CCTNAGC 2 cut(s) 1372, 1689
Bpu14I TTCGAA 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 1628
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 1802
BsaBI GATNNNNATC 1 cut(s) 551
BsaHI GRCGYC 1 cut(s) 489
BsaWI WCCGGW 2 cut(s) 1076, 1728
BsaXI ACNNNNNCTCC 4 cut(s) 578, 608, 1869, 1899
Bsc4I CCNNNNNNNGG 5 cut(s) 421, 1271, 1376, 1715, 1760
Bse1I ACTGG 6 cut(s) 95, 372, 481, 1405, 1813, 2030
Bse3DI GCAATG 1 cut(s) 1307
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 7 cut(s) 178, 398, 615, 1467, 1716, 1766, 1962
BseGI GGATG 5 cut(s) 281, 911, 1971, 2080, 2203
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 5 cut(s) 421, 1271, 1376, 1715, 1760
BseMI GCAATG 1 cut(s) 1307
BseMII CTCAG 3 cut(s) 213, 786, 1680
BseNI ACTGG 6 cut(s) 95, 372, 481, 1405, 1813, 2030
BseRI GAGGAG 4 cut(s) 812, 875, 2085, 2088
BseXI GCAGC 7 cut(s) 368, 735, 877, 951, 1107, 1291, 2050
BseYI CCCAGC 1 cut(s) 1513
Bsh1236I CGCG 7 cut(s) 265, 290, 327, 345, 537, 543, 1941
Bsh1285I CGRYCG 2 cut(s) 1420, 1729
BshFI GGCC 5 cut(s) 129, 235, 368, 1275, 1764
BshNI GGYRCC 2 cut(s) 488, 1388
BsiEI CGRYCG 2 cut(s) 1420, 1729
BsiHKAI GWGCWC 1 cut(s) 2001
BsiHKCI CYCGRG 2 cut(s) 530, 2002
BsiSI CCGG 4 cut(s) 22, 690, 1077, 1729
BslFI GGGAC 3 cut(s) 3, 1839, 2021
BslI CCNNNNNNNGG 5 cut(s) 421, 1271, 1376, 1715, 1760
BsmAI GTCTC 5 cut(s) 217, 1044, 1289, 1567, 1964
BsmFI GGGAC 3 cut(s) 3, 1839, 2021
BsmI GAATGC 1 cut(s) 1098
BsnI GGCC 5 cut(s) 129, 235, 368, 1275, 1764
BsoBI CYCGRG 2 cut(s) 530, 2002
Bsp119I TTCGAA 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 2001
Bsp1407I TGTACA 1 cut(s) 1581
Bsp143I GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
Bsp19I CCATGG 1 cut(s) 513
BspANI GGCC 5 cut(s) 129, 235, 368, 1275, 1764
BspCNI CTCAG 3 cut(s) 212, 787, 1681
BspFNI CGCG 7 cut(s) 265, 290, 327, 345, 537, 543, 1941
BspLI GGNNCC 9 cut(s) 19, 234, 402, 490, 1329, 1390, 1707, 1763, 1855
BspMAI CTGCAG 1 cut(s) 790
BspOI GCTAGC 1 cut(s) 2070
BspPI GGATC 6 cut(s) 74, 395, 408, 926, 1322, 1335
BspQI GCTCTTC 1 cut(s) 1638
BspT104I TTCGAA 1 cut(s) 100
BspT107I GGYRCC 2 cut(s) 488, 1388
BsrBI CCGCTC 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 1307
BsrGI TGTACA 1 cut(s) 1581
BsrI ACTGG 6 cut(s) 95, 372, 481, 1405, 1813, 2030
BssMI GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
BssNI GRCGYC 1 cut(s) 489
BssT1I CCWWGG 4 cut(s) 513, 561, 1060, 1858
Bst2UI CCWGG 7 cut(s) 178, 398, 615, 1467, 1716, 1766, 1962
Bst4CI ACNGT 8 cut(s) 62, 85, 112, 1282, 1318, 1388, 1823, 2178
Bst6I CTCTTC 2 cut(s) 283, 1638
BstACI GRCGYC 1 cut(s) 489
BstAUI TGTACA 1 cut(s) 1581
BstBAI YACGTR 1 cut(s) 1802
BstBI TTCGAA 1 cut(s) 100
BstDEI CTNAG 4 cut(s) 199, 795, 1372, 1689
BstDSI CCRYGG 5 cut(s) 111, 360, 445, 513, 578
BstENI CCTNNNNNAGG 1 cut(s) 1713
BstF5I GGATG 5 cut(s) 281, 911, 1971, 2080, 2203
BstFNI CGCG 7 cut(s) 265, 290, 327, 345, 537, 543, 1941
BstH2I RGCGCY 2 cut(s) 492, 1725
BstHHI GCGC 8 cut(s) 267, 329, 347, 491, 537, 543, 753, 1724
BstKTI GATC 8 cut(s) 69, 403, 781, 835, 934, 1330, 1420, 2017
BstMAI GTCTC 5 cut(s) 217, 1044, 1289, 1567, 1964
BstMBI GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
BstMCI CGRYCG 2 cut(s) 1420, 1729
BstNI CCWGG 7 cut(s) 178, 398, 615, 1467, 1716, 1766, 1962
BstNSI RCATGY 4 cut(s) 623, 958, 1652, 1752
BstSCI CCNGG 8 cut(s) 20, 176, 396, 613, 1465, 1714, 1764, 1960
BstSFI CTRYAG 2 cut(s) 786, 855
BstUI CGCG 7 cut(s) 265, 290, 327, 345, 537, 543, 1941
BstV1I GCAGC 7 cut(s) 368, 735, 877, 951, 1107, 1291, 2050
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 5 cut(s) 66, 400, 931, 1327, 2014
BstXI CCANNNNNNTGG 1 cut(s) 1400
BstYI RGATCY 5 cut(s) 66, 400, 931, 1327, 2014
BsuRI GGCC 5 cut(s) 129, 235, 368, 1275, 1764
BtgI CCRYGG 5 cut(s) 111, 360, 445, 513, 578
BtsCI GGATG 5 cut(s) 281, 911, 1971, 2080, 2203
BtsI GCAGTG 1 cut(s) 1798
BtsIMutI CAGTG 5 cut(s) 58, 379, 488, 1398, 1798
CaiI CAGNNNCTG 1 cut(s) 794
CfoI GCGC 8 cut(s) 267, 329, 347, 491, 537, 543, 753, 1724
Cfr13I GGNCC 8 cut(s) 17, 128, 233, 466, 698, 1706, 1762, 1853
CseI GACGC 2 cut(s) 279, 466
Csp6I GTAC 4 cut(s) 722, 1389, 1582, 1902
CviQI GTAC 4 cut(s) 722, 1389, 1582, 1902
DdeI CTNAG 4 cut(s) 199, 795, 1372, 1689
DinI GGCGCC 1 cut(s) 490
DpnI GATC 8 cut(s) 68, 402, 780, 834, 933, 1329, 1419, 2016
DpnII GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
DrdI GACNNNNNNGTC 1 cut(s) 1738
DriI GACNNNNNGTC 1 cut(s) 1240
DseDI GACNNNNNNGTC 1 cut(s) 1738
EaeI YGGCCR 1 cut(s) 1273
Eam1104I CTCTTC 2 cut(s) 283, 1638
Eam1105I GACNNNNNGTC 1 cut(s) 1240
EarI CTCTTC 2 cut(s) 283, 1638
EciI GGCGGA 1 cut(s) 467
Eco130I CCWWGG 4 cut(s) 513, 561, 1060, 1858
Eco47I GGWCC 5 cut(s) 17, 466, 698, 1706, 1853
Eco57I CTGAAG 2 cut(s) 1452, 1493
Eco72I CACGTG 1 cut(s) 1802
Eco88I CYCGRG 2 cut(s) 530, 2002
EcoNI CCTNNNNNAGG 1 cut(s) 1713
EcoO109I RGGNCCY 5 cut(s) 17, 233, 1706, 1762, 1853
EcoRII CCWGG 7 cut(s) 176, 396, 613, 1465, 1714, 1764, 1960
EcoT14I CCWWGG 4 cut(s) 513, 561, 1060, 1858
EcoT22I ATGCAT 1 cut(s) 316
EgeI GGCGCC 1 cut(s) 490
EheI GGCGCC 1 cut(s) 490
ErhI CCWWGG 4 cut(s) 513, 561, 1060, 1858
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FaqI GGGAC 3 cut(s) 3, 1839, 2021
FauI CCCGC 5 cut(s) 223, 536, 639, 1243, 1533
FauNDI CATATG 2 cut(s) 1203, 1975
FblI GTMKAC 1 cut(s) 441
FokI GGATG 5 cut(s) 268, 918, 1978, 2087, 2210
FspBI CTAG 4 cut(s) 869, 1734, 2018, 2067
GlaI GCGC 8 cut(s) 266, 328, 346, 490, 536, 542, 752, 1723
GsaI CCCAGC 1 cut(s) 1517
GsuI CTGGAG 4 cut(s) 355, 1164, 1245, 1422
HaeII RGCGCY 2 cut(s) 492, 1725
HaeIII GGCC 5 cut(s) 129, 235, 368, 1275, 1764
HapII CCGG 4 cut(s) 22, 690, 1077, 1729
HgaI GACGC 2 cut(s) 279, 466
HhaI GCGC 8 cut(s) 267, 329, 347, 491, 537, 543, 753, 1724
Hin1I GRCGYC 1 cut(s) 489
Hin6I GCGC 8 cut(s) 265, 327, 345, 489, 535, 541, 751, 1722
HinP1I GCGC 8 cut(s) 265, 327, 345, 489, 535, 541, 751, 1722
HincII GTYRAC 3 cut(s) 442, 2026, 2203
HindII GTYRAC 3 cut(s) 442, 2026, 2203
HinfI GANTC 6 cut(s) 518, 844, 1139, 1364, 1478, 2110
HpaI GTTAAC 1 cut(s) 2203
HpaII CCGG 4 cut(s) 22, 690, 1077, 1729
HphI GGTGA 8 cut(s) 73, 100, 375, 647, 916, 1121, 1244, 1557
Hpy166II GTNNAC 5 cut(s) 58, 294, 442, 2026, 2203
Hpy188I TCNGA 4 cut(s) 405, 796, 1477, 2143
Hpy188III TCNNGA 6 cut(s) 1143, 1382, 1870, 2018, 2114, 2195
Hpy8I GTNNAC 5 cut(s) 58, 294, 442, 2026, 2203
Hpy99I CGWCG 1 cut(s) 460
HpyAV CCTTC 9 cut(s) 283, 358, 577, 689, 896, 1157, 1162, 1186, 1538
HpyCH4III ACNGT 8 cut(s) 62, 85, 112, 1282, 1318, 1388, 1823, 2178
HpyCH4IV ACGT 3 cut(s) 161, 420, 1801
HpyF3I CTNAG 4 cut(s) 199, 795, 1372, 1689
HpySE526I ACGT 3 cut(s) 161, 420, 1801
Hsp92I GRCGYC 1 cut(s) 489
HspAI GCGC 8 cut(s) 265, 327, 345, 489, 535, 541, 751, 1722
KasI GGCGCC 1 cut(s) 488
KpnI GGTACC 1 cut(s) 1392
KspAI GTTAAC 1 cut(s) 2203
Kzo9I GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
LguI GCTCTTC 1 cut(s) 1638
LmnI GCTCC 5 cut(s) 398, 480, 643, 862, 1226
Lsp1109I GCAGC 7 cut(s) 368, 735, 877, 951, 1107, 1291, 2050
LweI GCATC 4 cut(s) 338, 676, 934, 961
MaeI CTAG 4 cut(s) 869, 1734, 2018, 2067
MaeII ACGT 3 cut(s) 161, 420, 1801
MalI GATC 8 cut(s) 68, 402, 780, 834, 933, 1329, 1419, 2016
MbiI CCGCTC 1 cut(s) 646
MboI GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
MfeI CAATTG 2 cut(s) 182, 1614
MflI RGATCY 5 cut(s) 66, 400, 931, 1327, 2014
MhlI GDGCHC 1 cut(s) 2001
MlsI TGGCCA 1 cut(s) 1275
MluCI AATT 7 cut(s) 182, 573, 1155, 1212, 1410, 1524, 1614
MluNI TGGCCA 1 cut(s) 1275
Mly113I GGCGCC 1 cut(s) 489
MlyI GAGTC 2 cut(s) 1358, 2104
MmeI TCCRAC 3 cut(s) 44, 67, 1305
Mox20I TGGCCA 1 cut(s) 1275
Mph1103I ATGCAT 1 cut(s) 316
MscI TGGCCA 1 cut(s) 1275
MslI CAYNNNNRTG 3 cut(s) 113, 1319, 1747
Msp20I TGGCCA 1 cut(s) 1275
MspA1I CMGCKG 2 cut(s) 1430, 1513
MspI CCGG 4 cut(s) 22, 690, 1077, 1729
MspR9I CCNGG 8 cut(s) 22, 178, 398, 615, 1467, 1716, 1766, 1962
MunI CAATTG 2 cut(s) 182, 1614
Mva1269I GAATGC 1 cut(s) 1098
MvaI CCWGG 7 cut(s) 178, 398, 615, 1467, 1716, 1766, 1962
MvnI CGCG 7 cut(s) 265, 290, 327, 345, 537, 543, 1941
NarI GGCGCC 1 cut(s) 489
NciI CCSGG 1 cut(s) 22
NcoI CCATGG 1 cut(s) 513
NdeI CATATG 2 cut(s) 1203, 1975
NdeII GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
NheI GCTAGC 1 cut(s) 2066
NlaIV GGNNCC 9 cut(s) 19, 234, 402, 490, 1329, 1390, 1707, 1763, 1855
NmeAIII GCCGAG 1 cut(s) 332
NmuCI GTSAC 7 cut(s) 957, 1109, 1130, 1232, 1382, 1793, 1954
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 4 cut(s) 623, 958, 1652, 1752
NspV TTCGAA 1 cut(s) 100
OliI CACNNNNGTG 2 cut(s) 113, 1319
PaeR7I CTCGAG 2 cut(s) 530, 2002
PciI ACATGT 2 cut(s) 954, 1748
PciSI GCTCTTC 1 cut(s) 1638
PctI GAATGC 1 cut(s) 1098
PfeI GAWTC 4 cut(s) 518, 844, 1139, 1478
PflMI CCANNNNNTGG 1 cut(s) 1271
PfoI TCCNGGA 1 cut(s) 1714
Ple19I CGATCG 1 cut(s) 1420
PleI GAGTC 2 cut(s) 1358, 2104
PluTI GGCGCC 1 cut(s) 492
PmaCI CACGTG 1 cut(s) 1802
PmlI CACGTG 1 cut(s) 1802
PpsI GAGTC 2 cut(s) 1358, 2104
Ppu21I YACGTR 1 cut(s) 1802
PpuMI RGGWCCY 3 cut(s) 17, 1706, 1853
PscI ACATGT 2 cut(s) 954, 1748
PshBI ATTAAT 1 cut(s) 1455
PsiI TTATAA 1 cut(s) 980
Psp1406I AACGTT 1 cut(s) 161
Psp5II RGGWCCY 3 cut(s) 17, 1706, 1853
Psp6I CCWGG 7 cut(s) 176, 396, 613, 1465, 1714, 1764, 1960
PspCI CACGTG 1 cut(s) 1802
PspFI CCCAGC 1 cut(s) 1513
PspGI CCWGG 7 cut(s) 176, 396, 613, 1465, 1714, 1764, 1960
PspN4I GGNNCC 9 cut(s) 19, 234, 402, 490, 1329, 1390, 1707, 1763, 1855
PspPI GGNCC 8 cut(s) 17, 128, 233, 466, 698, 1706, 1762, 1853
PspPPI RGGWCCY 3 cut(s) 17, 1706, 1853
PspXI VCTCGAGB 2 cut(s) 530, 2002
PstI CTGCAG 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 5 cut(s) 66, 400, 931, 1327, 2014
PvuI CGATCG 1 cut(s) 1420
PvuII CAGCTG 2 cut(s) 1430, 1513
RsaI GTAC 4 cut(s) 723, 1390, 1583, 1903
RsaNI GTAC 4 cut(s) 722, 1389, 1582, 1902
RseI CAYNNNNRTG 3 cut(s) 113, 1319, 1747
SalI GTCGAC 1 cut(s) 440
SapI GCTCTTC 1 cut(s) 1638
Sau3AI GATC 8 cut(s) 66, 400, 778, 832, 931, 1327, 1417, 2014
Sau96I GGNCC 8 cut(s) 17, 128, 233, 466, 698, 1706, 1762, 1853
SchI GAGTC 2 cut(s) 1358, 2104
ScrFI CCNGG 8 cut(s) 22, 178, 398, 615, 1467, 1716, 1766, 1962
SduI GDGCHC 1 cut(s) 2001
SfaNI GCATC 4 cut(s) 338, 676, 934, 961
SfcI CTRYAG 2 cut(s) 786, 855
SfoI GGCGCC 1 cut(s) 490
Sfr274I CTCGAG 2 cut(s) 530, 2002
SfuI TTCGAA 1 cut(s) 100
SinI GGWCC 5 cut(s) 17, 466, 698, 1706, 1853
SlaI CTCGAG 2 cut(s) 530, 2002
SmiMI CAYNNNNRTG 3 cut(s) 113, 1319, 1747
SmlI CTYRAG 3 cut(s) 530, 1607, 2002
SmoI CTYRAG 3 cut(s) 530, 1607, 2002
Sse9I AATT 7 cut(s) 182, 573, 1155, 1212, 1410, 1524, 1614
SspDI GGCGCC 1 cut(s) 488
SspI AATATT 1 cut(s) 1071
SspMI CTAG 4 cut(s) 869, 1734, 2018, 2067
StyD4I CCNGG 8 cut(s) 20, 176, 396, 613, 1465, 1714, 1764, 1960
StyI CCWWGG 4 cut(s) 513, 561, 1060, 1858
TaaI ACNGT 8 cut(s) 62, 85, 112, 1282, 1318, 1388, 1823, 2178
TaiI ACGT 3 cut(s) 164, 423, 1804
TaqI TCGA 7 cut(s) 100, 441, 531, 914, 1420, 1849, 2003
TasI AATT 7 cut(s) 182, 573, 1155, 1212, 1410, 1524, 1614
TatI WGTACW 2 cut(s) 721, 1581
TauI GCSGC 3 cut(s) 368, 411, 489
TfiI GAWTC 4 cut(s) 518, 844, 1139, 1478
TscAI CASTG 5 cut(s) 65, 379, 488, 1405, 1798
TseFI GTSAC 7 cut(s) 957, 1109, 1130, 1232, 1382, 1793, 1954
TseI GCWGC 7 cut(s) 356, 748, 865, 964, 1095, 1304, 2063
Tsp45I GTSAC 7 cut(s) 957, 1109, 1130, 1232, 1382, 1793, 1954
TspDTI ATGAA 7 cut(s) 270, 825, 1109, 1199, 1656, 1854, 2160
TspGWI ACGGA 1 cut(s) 567
TspRI CASTG 5 cut(s) 65, 379, 488, 1405, 1798
Van91I CCANNNNNTGG 1 cut(s) 1271
VpaK11BI GGWCC 5 cut(s) 17, 466, 698, 1706, 1853
VspI ATTAAT 1 cut(s) 1455
XagI CCTNNNNNAGG 1 cut(s) 1713
XbaI TCTAGA 1 cut(s) 2017
XceI RCATGY 4 cut(s) 623, 958, 1652, 1752
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 1321
XhoI CTCGAG 2 cut(s) 530, 2002
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 4 cut(s) 869, 1734, 2018, 2067
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.