pycom17g24200

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0017722
Physical Location & Seq
Forward (+)
22513678 .. 22516466
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGGAGGTTTGGGAAGAGGAACTGGAGCCAGTGAGCCCTACTGGCCACTATTTCAACAGCTCTGTTTTATCGGTATCAATTCTTGCTATTTTGGAAAATGAATTTCCAATTGATGACTCTCAATTTTTGTCATTGCTCGAGAATGTGTTCCTGCCCATCAACCCACGCTTCTCCTCTATAATGATTGAAAAAAATGGAAAGAAACAATGGAAAAAGGTTGAAGTGAAGCTTGAAGACCACGTTAAAACTCCAATCTTCCCTTCCAATTTGTCGAATGAATCTTATGACGAGTATTTTGATGACTATATCTCAAGCATAGCTACTAAAAGATTTCCACAAGACAAACCACTGTGGGAAGTTCATGTTATAAAGTACCCAAATAGCAATGCAGCTGGTAATGTAATATTCAAGTTGCACCATGCATTAGGTGATGGCTACTCTCTCATGGGCGCTCTTCTCTCCTGTCTACAAAGGGCTGACAATCCTTCTCTTCCACTAACTTTTCCTTCACTGCTGCCATCAGAAACGAAGAGTGAAAATGTTATGAACAAAACTATCTCATCCTTGTTGAGCACCATATCAGATTTTTGGTGGGGCATTTCAAAGAGCTTTAGGGGGGAAGATGATCCATCACCAATAAAATCCTCGAAAAATGGACTTGAGTTCGCGCCAATTACAATATCAACGATTATGATCTCCCTTGATCGAATCAGATTAATTAAGAGCAAACTCGGAGTGGTAAACGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

251

Amino Acids

28.21

Weight (kDa)

5.19

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 67 - 186 7.7e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AccI GTMKAC 1 cut(s) 466
AccII CGCG 1 cut(s) 668
AclWI GGATC 1 cut(s) 620
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 1 cut(s) 101
AfaI GTAC 1 cut(s) 374
AgsI TTSAA 6 cut(s) 55, 188, 221, 233, 409, 603
AjuI GAANNNNNNNTTGG 2 cut(s) 244, 276
AluBI AGCT 5 cut(s) 60, 229, 320, 392, 609
AluI AGCT 5 cut(s) 60, 229, 320, 392, 609
Alw21I GWGCWC 1 cut(s) 575
AlwI GGATC 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 2 cut(s) 389, 514
ApoI RAATTY 1 cut(s) 101
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AseI ATTAAT 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 146
AspLEI GCGC 2 cut(s) 452, 670
AsuHPI GGTGA 2 cut(s) 440, 624
AvaI CYCGRG 1 cut(s) 137
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 2 cut(s) 401, 501
BccI CCATC 4 cut(s) 164, 425, 526, 637
BfoI RGCGCY 1 cut(s) 453
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 2 cut(s) 390, 515
BlsI GCNGC 2 cut(s) 391, 516
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 27
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 2 cut(s) 295, 680
BsaBI GATNNNNATC 1 cut(s) 692
Bse1I ACTGG 3 cut(s) 27, 29, 46
Bse3DI GCAATG 2 cut(s) 131, 391
Bse8I GATNNNNATC 1 cut(s) 692
BseGI GGATG 1 cut(s) 560
BseJI GATNNNNATC 1 cut(s) 692
BseMI GCAATG 2 cut(s) 131, 391
BseNI ACTGG 3 cut(s) 27, 29, 46
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 2 cut(s) 401, 501
Bsh1236I CGCG 1 cut(s) 668
BshFI GGCC 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 575
BsiHKCI CYCGRG 1 cut(s) 137
BsnI GGCC 1 cut(s) 45
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 38, 575
Bsp143I GATC 4 cut(s) 625, 693, 703, 745
BspANI GGCC 1 cut(s) 45
BspFNI CGCG 1 cut(s) 668
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 1 cut(s) 459
BsrDI GCAATG 2 cut(s) 131, 391
BsrI ACTGG 3 cut(s) 27, 29, 46
BssMI GATC 4 cut(s) 625, 693, 703, 745
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 4 cut(s) 9, 459, 495, 524
BstF5I GGATG 1 cut(s) 560
BstFNI CGCG 1 cut(s) 668
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 452, 670
BstKTI GATC 4 cut(s) 628, 696, 706, 748
BstMBI GATC 4 cut(s) 625, 693, 703, 745
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 2 cut(s) 401, 501
BstV2I GAAGAC 1 cut(s) 240
BsuRI GGCC 1 cut(s) 45
BtsCI GGATG 1 cut(s) 560
BtsI GCAGTG 1 cut(s) 509
BtsIMutI CAGTG 3 cut(s) 36, 347, 509
CfoI GCGC 2 cut(s) 452, 670
Csp6I GTAC 1 cut(s) 373
CviAII CATG 3 cut(s) 362, 419, 445
CviQI GTAC 1 cut(s) 373
DpnI GATC 4 cut(s) 627, 695, 705, 747
DpnII GATC 4 cut(s) 625, 693, 703, 745
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 4 cut(s) 9, 459, 495, 524
EarI CTCTTC 4 cut(s) 9, 459, 495, 524
Eco24I GRGCYC 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 38
FaeI CATG 3 cut(s) 365, 422, 448
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FatI CATG 3 cut(s) 361, 418, 444
FblI GTMKAC 1 cut(s) 466
Fnu4HI GCNGC 2 cut(s) 390, 515
FokI GGATG 1 cut(s) 547
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 2 cut(s) 390, 515
GlaI GCGC 2 cut(s) 451, 669
GluI GCNGC 2 cut(s) 390, 515
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 1 cut(s) 45
HhaI GCGC 2 cut(s) 452, 670
Hin1II CATG 3 cut(s) 365, 422, 448
Hin6I GCGC 2 cut(s) 450, 668
HinP1I GCGC 2 cut(s) 450, 668
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 3 cut(s) 116, 278, 708
HphI GGTGA 2 cut(s) 440, 624
Hpy166II GTNNAC 2 cut(s) 467, 742
Hpy188I TCNGA 4 cut(s) 523, 583, 713, 734
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 2 cut(s) 467, 742
HpyAV CCTTC 3 cut(s) 270, 495, 516
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 389, 415, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 3 cut(s) 365, 422, 448
HspAI GCGC 2 cut(s) 450, 668
Kzo9I GATC 4 cut(s) 625, 693, 703, 745
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 6 cut(s) 8, 27, 42, 164, 378, 475
Lsp1109I GCAGC 2 cut(s) 401, 501
MaeII ACGT 1 cut(s) 240
MalI GATC 4 cut(s) 627, 695, 705, 747
MboI GATC 4 cut(s) 625, 693, 703, 745
MboII GAAGA 7 cut(s) 26, 245, 247, 446, 482, 541, 632
MfeI CAATTG 1 cut(s) 108
MhlI GDGCHC 2 cut(s) 38, 575
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 7 cut(s) 78, 101, 108, 122, 265, 672, 717
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 3 cut(s) 10, 184, 655
Mox20I TGGCCA 1 cut(s) 45
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 146
MscI TGGCCA 1 cut(s) 45
MseI TTAA 3 cut(s) 243, 716, 720
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 392
MunI CAATTG 1 cut(s) 108
MvnI CGCG 1 cut(s) 668
MwoI GCNNNNNNNGC 1 cut(s) 42
NdeII GATC 4 cut(s) 625, 693, 703, 745
NlaIII CATG 3 cut(s) 365, 422, 448
NlaIV GGNNCC 1 cut(s) 27
NsiI ATGCAT 1 cut(s) 424
PacI TTAATTAA 1 cut(s) 720
PaeR7I CTCGAG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 459
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 2 cut(s) 278, 708
PkrI GCNGC 2 cut(s) 391, 516
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 716
PsiI TTATAA 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 27
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 243, 716, 720
SatI GCNGC 2 cut(s) 390, 515
Sau3AI GATC 4 cut(s) 625, 693, 703, 745
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 2 cut(s) 38, 575
SetI ASST 9 cut(s) 9, 62, 219, 231, 243, 322, 394, 430, 611
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 3 cut(s) 137, 310, 659
SmoI CTYRAG 3 cut(s) 137, 310, 659
Sse9I AATT 7 cut(s) 78, 101, 108, 122, 265, 672, 717
SspI AATATT 1 cut(s) 405
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 1 cut(s) 243
TaqI TCGA 4 cut(s) 138, 272, 647, 706
TasI AATT 7 cut(s) 78, 101, 108, 122, 265, 672, 717
TfiI GAWTC 2 cut(s) 278, 708
Tru1I TTAA 3 cut(s) 243, 716, 720
Tru9I TTAA 3 cut(s) 243, 716, 720
TscAI CASTG 3 cut(s) 36, 354, 516
TseI GCWGC 2 cut(s) 389, 514
TspDTI ATGAA 4 cut(s) 114, 291, 350, 560
TspRI CASTG 3 cut(s) 36, 354, 516
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 1 cut(s) 101
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.