pycom17g26660

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0018495
Physical Location & Seq
Forward (+)
24957212 .. 24958495
1284 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGCAGGCTTGTGGTGGAGCTGCAGTGATGGGTTCTCTTCAACAACCCGCTTGGACCAAAGGAACAATTTTTTCAAACAAGGGGCTTACCGGCAGTGGATTTTCCCATCAACTAAACCTCAATTGTGTGAAGCCCGTTAGATCTTCTTACATAGAAGGAAGCTTGGTTACTGGAAGGCCATCCTCTTCTGTCACTGTACCTTTGCCTGAAATTGGTGGTAATGGAAGCAGTTTTGTAGACAACGGATTAAGTGAGGCTGACCCTGAGGTGCGTGCTATTATTGATATGGAGAAGCAGCGACAGTTCAAGAGTCTTGAGCTTATTGCCTCAGAAAATTTTACTTATCGGGCAGTGATGGAGGCAGTTGGCTCATGTCTAACGAACAAGTATTCAGAAGGTTTACCAGGTAAAAGGTACTATGGTGGCAATGAGCATATTGATGAGCTTGAAACACTTTGCCAAAAGAGGGCTCTTGATGCATTTCACTTAGATGGAAAGAAGTGGGGTGTTAATGTTCAACCATTATCTGGTTCCCCTGCTAATTTCGAGGTTTATACCGCAATTCTTAAACCTCATCGATCGTCTCATGGGTTTGGACTTGTCTCACGGAGGACATTTGTCTCATGGTTTCATGACTCCTAA

Protein Analysis

214

Amino Acids

23.15

Weight (kDa)

7.71

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 83 - 198 8.4e-53 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018495)

Species Orthologous Gene IDs
malus_domestica MD09G1269200.v1.1
pyrus_communis pycom17g26660
rosa_chinensis RchiOBHm_Chr1g0360671 RchiOBHm_Chr7g0223221
rosa_multiflora Rmu_co8421897.1_g000001
rosa_roxburghii Rroxscaffold_4G00301080 Rroxscaffold_7G00212560
rosa_samantha Rh3BG358300 Rh6AG220400
rosa_wichuraiana Rw6G019320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 527
AccI GTMKAC 1 cut(s) 237
AciI CCGC 2 cut(s) 48, 558
AcsI RAATTY 1 cut(s) 334
AfaI GTAC 2 cut(s) 198, 416
AfiI CCNNNNNNNGG 3 cut(s) 212, 466, 527
AgsI TTSAA 5 cut(s) 41, 75, 307, 449, 518
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 4 cut(s) 20, 162, 319, 445
AluI AGCT 4 cut(s) 20, 162, 319, 445
Alw26I GTCTC 3 cut(s) 588, 607, 625
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 2 cut(s) 20, 295
ApoI RAATTY 1 cut(s) 334
AspS9I GGNCC 1 cut(s) 54
AvaII GGWCC 1 cut(s) 54
AxyI CCTNAGG 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 406, 439
BanII GRGCYC 1 cut(s) 472
BarI GAAGNNNNNNTAC 2 cut(s) 151, 183
BbvI GCAGC 2 cut(s) 7, 307
BccI CCATC 5 cut(s) 22, 114, 187, 349, 485
BciT130I CCWGG 1 cut(s) 405
BcoDI GTCTC 3 cut(s) 588, 607, 625
BfmI CTRYAG 1 cut(s) 21
BglII AGATCT 1 cut(s) 140
BisI GCNGC 2 cut(s) 21, 296
BlsI GCNGC 2 cut(s) 22, 297
Bme1390I CCNGG 1 cut(s) 405
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 532
BmrFI CCNGG 1 cut(s) 405
BmsI GCATC 1 cut(s) 466
BoxI GACNNNNGTC 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 335
Bsa29I ATCGAT 1 cut(s) 577
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 466, 527
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 175
Bse21I CCTNAGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 405
BseCI ATCGAT 1 cut(s) 577
BseGI GGATG 1 cut(s) 179
BseLI CCNNNNNNNGG 3 cut(s) 212, 466, 527
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 2 cut(s) 255, 342
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 7, 307
Bsh1285I CGRYCG 1 cut(s) 581
BshFI GGCC 1 cut(s) 178
BshVI ATCGAT 1 cut(s) 577
BsiEI CGRYCG 1 cut(s) 581
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 3 cut(s) 212, 466, 527
BsmAI GTCTC 3 cut(s) 588, 607, 625
BsmBI CGTCTC 1 cut(s) 588
BsnI GGCC 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 472
Bsp143I GATC 2 cut(s) 140, 578
BspACI CCGC 2 cut(s) 48, 558
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 2 cut(s) 256, 341
BspDI ATCGAT 1 cut(s) 577
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 532
BspMAI CTGCAG 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 1 cut(s) 175
BssAI RCCGGY 1 cut(s) 89
BssMI GATC 2 cut(s) 140, 578
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 196, 303
Bst6I CTCTTC 2 cut(s) 42, 190
BstC8I GCNNGC 2 cut(s) 6, 273
BstDEI CTNAG 3 cut(s) 264, 328, 487
BstF5I GGATG 1 cut(s) 179
BstKTI GATC 2 cut(s) 143, 581
BstMAI GTCTC 3 cut(s) 588, 607, 625
BstMBI GATC 2 cut(s) 140, 578
BstMCI CGRYCG 1 cut(s) 581
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 1 cut(s) 405
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 403
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 7, 307
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
Bsu15I ATCGAT 1 cut(s) 577
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 1 cut(s) 178
BsuTUI ATCGAT 1 cut(s) 577
BtsCI GGATG 1 cut(s) 179
BtsI GCAGTG 3 cut(s) 30, 100, 357
BtsIMutI CAGTG 4 cut(s) 30, 100, 192, 357
Cac8I GCNNGC 2 cut(s) 6, 273
CciI TCATGA 1 cut(s) 631
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 577
CsiI ACCWGGT 1 cut(s) 403
Csp6I GTAC 2 cut(s) 197, 415
CviAII CATG 4 cut(s) 372, 587, 624, 632
CviQI GTAC 2 cut(s) 197, 415
DdeI CTNAG 3 cut(s) 264, 328, 487
DpnI GATC 2 cut(s) 142, 580
DpnII GATC 2 cut(s) 140, 578
Eam1104I CTCTTC 2 cut(s) 42, 190
EarI CTCTTC 2 cut(s) 42, 190
Eco24I GRGCYC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 403
EcoT22I ATGCAT 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 472
Esp3I CGTCTC 1 cut(s) 588
FaeI CATG 4 cut(s) 375, 590, 627, 635
FaiI YATR 9 cut(s) 152, 287, 373, 420, 435, 555, 588, 625, 633
FatI CATG 4 cut(s) 371, 586, 623, 631
FauI CCCGC 1 cut(s) 55
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 2 cut(s) 21, 296
FokI GGATG 1 cut(s) 166
FriOI GRGCYC 1 cut(s) 472
Fsp4HI GCNGC 2 cut(s) 21, 296
GluI GCNGC 2 cut(s) 21, 296
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 90
Hin1II CATG 4 cut(s) 375, 590, 627, 635
HindIII AAGCTT 1 cut(s) 160
HinfI GANTC 2 cut(s) 310, 635
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 2 cut(s) 238, 401
Hpy188I TCNGA 2 cut(s) 331, 394
Hpy188III TCNNGA 4 cut(s) 307, 314, 473, 632
Hpy8I GTNNAC 2 cut(s) 238, 401
HpyAV CCTTC 3 cut(s) 149, 168, 389
HpyCH4III ACNGT 2 cut(s) 196, 303
HpyCH4V TGCA 3 cut(s) 4, 23, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 3 cut(s) 264, 328, 487
Hsp92II CATG 4 cut(s) 375, 590, 627, 635
Kzo9I GATC 2 cut(s) 140, 578
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 8 cut(s) 103, 156, 219, 276, 390, 417, 513, 549
Lsp1109I GCAGC 2 cut(s) 7, 307
LweI GCATC 1 cut(s) 466
MabI ACCWGGT 1 cut(s) 403
MaeIII GTNAC 2 cut(s) 166, 190
MalI GATC 2 cut(s) 142, 580
MboI GATC 2 cut(s) 140, 578
MboII GAAGA 3 cut(s) 29, 135, 177
MfeI CAATTG 1 cut(s) 121
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 1 cut(s) 472
MluCI AATT 6 cut(s) 66, 121, 210, 334, 541, 561
MlyI GAGTC 2 cut(s) 319, 629
Mph1103I ATGCAT 1 cut(s) 481
MseI TTAA 3 cut(s) 248, 510, 567
MslI CAYNNNNRTG 2 cut(s) 438, 489
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 405
MunI CAATTG 1 cut(s) 121
MvaI CCWGG 1 cut(s) 405
MwoI GCNNNNNNNGC 1 cut(s) 476
NdeII GATC 2 cut(s) 140, 578
NlaIII CATG 4 cut(s) 375, 590, 627, 635
NlaIV GGNNCC 1 cut(s) 532
NmuCI GTSAC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 481
PagI TCATGA 1 cut(s) 631
PflMI CCANNNNNTGG 1 cut(s) 527
PkrI GCNGC 2 cut(s) 22, 297
Ple19I CGATCG 1 cut(s) 581
PleI GAGTC 2 cut(s) 318, 629
PpsI GAGTC 2 cut(s) 318, 629
PshAI GACNNNNGTC 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 532
PspPI GGNCC 1 cut(s) 54
PstI CTGCAG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 140
PvuI CGATCG 1 cut(s) 581
RsaI GTAC 2 cut(s) 198, 416
RsaNI GTAC 2 cut(s) 197, 415
RseI CAYNNNNRTG 2 cut(s) 438, 489
SaqAI TTAA 3 cut(s) 248, 510, 567
SatI GCNGC 2 cut(s) 21, 296
Sau3AI GATC 2 cut(s) 140, 578
Sau96I GGNCC 1 cut(s) 54
SchI GAGTC 2 cut(s) 319, 629
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 1 cut(s) 472
SexAI ACCWGGT 1 cut(s) 403
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 1 cut(s) 21
SinI GGWCC 1 cut(s) 54
SmiMI CAYNNNNRTG 2 cut(s) 438, 489
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 6 cut(s) 66, 121, 210, 334, 541, 561
SsiI CCGC 2 cut(s) 48, 558
StyD4I CCNGG 1 cut(s) 403
TaaI ACNGT 2 cut(s) 196, 303
TaqI TCGA 2 cut(s) 546, 577
TasI AATT 6 cut(s) 66, 121, 210, 334, 541, 561
Tru1I TTAA 3 cut(s) 248, 510, 567
Tru9I TTAA 3 cut(s) 248, 510, 567
TscAI CASTG 4 cut(s) 30, 100, 199, 357
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 2 cut(s) 20, 295
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 620
TspGWI ACGGA 2 cut(s) 258, 622
TspRI CASTG 4 cut(s) 30, 100, 199, 357
Van91I CCANNNNNTGG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 54
XapI RAATTY 1 cut(s) 334
XmiI GTMKAC 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.