pycom215g00020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000215
OG0015353
Physical Location & Seq
Forward (+)
103746 .. 105179
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom215g00020.2

Sequence Viewer

Length: 579 bp
ATGGCGCTTTCAGTCTCTACCCAATCATCTGCGTTGAGATCGCTGTGGACAGCTGTGGGTCGGCGGCGTTCCTACGCCAAGGTGGCATCTTCTTCCTCTTATGCGGCGGAAGGGCGGAGGGAGAAAAAACAAGTAGAAGCAGAGGCAGAAGAAGGCGTCGACGTCGACGTCGGTGGGTTCGCAGTGAGCTCCGGAATCAGCAGACCCCTCTCTGAAATCCTCAAGCAACTCAACAAGAAAGTCCCCGATTCCCTCGTCAAAACCCGCTCTGAAAATGGCTTCACCTCCAAATACATCCCCTGGCACATTGTAAATCGGATTATGAACTTGCATGCTCCAGAATGGTCCGGTGAGGTTCGAAGCGTCACTTACTCTGCTGATGCCAAGTCTGTATCGGTTGTTTATCGCGTCACGCTCTACGGAACTGATGCTGAGATTTTTAGGGAGTCAATAGGCACTGCTTCAGTAGCTGACACAAGTTATGGGGATCCTGTACAGAAGGCAGAGGCAATGGCATTTCGTCGAGCTTGTGCACGCTTTGGTCTTGGACTTCATCTTTATCATGAGGACTTGTCGTAG

Protein Analysis

193

Amino Acids

21.03

Weight (kDa)

8.97

Isoelectric Point (pI)

56.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71310 AT1G71310 AT1G71310
fragaria_vesca FvH4_2g00170
malus_domestica MD05G1000800.v1.1
prunus_persica Prupe.8G001500_v2.0.a1
pyrus_communis pycom215g00020
rosa_chinensis RchiOBHm_Chr6g0249441
rosa_laevigata RLG00000015579
rosa_multiflora Rmu_ssc0000459.1_g000022
rosa_roxburghii Rroxscaffold_7G00217710
rosa_rugosa Rorug05G0492500
rosa_samantha Rh6AG002200 Rh6CG001400 Rh6DG001400
rosa_wichuraiana Rw6G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AatII GACGTC 2 cut(s) 165, 171
AccBSI CCGCTC 1 cut(s) 267
AccI GTMKAC 2 cut(s) 159, 165
AccII CGCG 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 191
AciI CCGC 5 cut(s) 64, 104, 107, 115, 265
AclWI GGATC 2 cut(s) 482, 495
AcuI CTGAAG 1 cut(s) 447
AcyI GRCGYC 3 cut(s) 156, 162, 168
AfaI GTAC 1 cut(s) 495
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 4 cut(s) 53, 189, 470, 527
AluI AGCT 4 cut(s) 53, 189, 470, 527
Alw21I GWGCWC 2 cut(s) 191, 535
Alw26I GTCTC 1 cut(s) 19
Alw44I GTGCAC 1 cut(s) 531
AlwI GGATC 2 cut(s) 482, 495
AlwNI CAGNNNCTG 1 cut(s) 470
Aor13HI TCCGGA 1 cut(s) 191
ApaLI GTGCAC 1 cut(s) 531
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 274, 362
AsuII TTCGAA 1 cut(s) 358
AvaII GGWCC 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 535
BamHI GGATCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 191
Bbv12I GWGCWC 2 cut(s) 191, 535
BciT130I CCWGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 19
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 83
BisI GCNGC 2 cut(s) 65, 105
BlsI GCNGC 2 cut(s) 66, 106
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 345
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 3 cut(s) 95, 370, 418
BpmI CTGGAG 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 358
BpuEI CTTGAG 1 cut(s) 206
BsaHI GRCGYC 3 cut(s) 156, 162, 168
BsaJI CCNNGG 2 cut(s) 78, 299
BsaWI WCCGGW 2 cut(s) 191, 347
Bse3DI GCAATG 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 191
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 2 cut(s) 78, 299
BseGI GGATG 1 cut(s) 294
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 535
Bsh1236I CGCG 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 191, 535
BsiSI CCGG 2 cut(s) 192, 348
BslFI GGGAC 1 cut(s) 227
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 358
Bsp1286I GDGCHC 2 cut(s) 191, 535
Bsp13I TCCGGA 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 493
Bsp143I GATC 2 cut(s) 38, 487
BspACI CCGC 5 cut(s) 64, 104, 107, 115, 265
BspCNI CTCAG 1 cut(s) 424
BspEI TCCGGA 1 cut(s) 191
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 1 cut(s) 489
BspPI GGATC 2 cut(s) 482, 495
BspT104I TTCGAA 1 cut(s) 358
BsrBI CCGCTC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 516
BsrGI TGTACA 1 cut(s) 493
BssECI CCNNGG 2 cut(s) 78, 299
BssMI GATC 2 cut(s) 38, 487
BssNI GRCGYC 3 cut(s) 156, 162, 168
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 301
BstACI GRCGYC 3 cut(s) 156, 162, 168
BstAUI TGTACA 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 333, 535
BstDEI CTNAG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 294
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 41, 490
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 2 cut(s) 38, 487
BstMWI GCNNNNNNNGC 2 cut(s) 83, 467
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 299
BstSLI GKGCMC 1 cut(s) 535
BstUI CGCG 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BtsCI GGATG 1 cut(s) 294
BtsI GCAGTG 2 cut(s) 189, 456
BtsIMutI CAGTG 2 cut(s) 189, 456
Cac8I GCNNGC 2 cut(s) 333, 535
CaiI CAGNNNCTG 1 cut(s) 470
CciI TCATGA 1 cut(s) 562
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 345
CseI GACGC 3 cut(s) 145, 352, 397
Csp6I GTAC 1 cut(s) 494
CviAII CATG 2 cut(s) 332, 563
CviJI RGCY 5 cut(s) 53, 189, 279, 470, 527
CviKI_1 RGCY 5 cut(s) 53, 189, 279, 470, 527
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 1 cut(s) 432
DpnI GATC 2 cut(s) 40, 489
DpnII GATC 2 cut(s) 38, 487
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EciI GGCGGA 2 cut(s) 122, 130
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 345
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 2 cut(s) 335, 566
FaiI YATR 5 cut(s) 102, 323, 333, 483, 564
FalI AAGNNNNNCTT 2 cut(s) 352, 384
FaqI GGGAC 1 cut(s) 227
FatI CATG 2 cut(s) 331, 562
FauI CCCGC 1 cut(s) 272
FblI GTMKAC 2 cut(s) 159, 165
Fnu4HI GCNGC 2 cut(s) 65, 105
FokI GGATG 1 cut(s) 281
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 65, 105
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 65, 105
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 2 cut(s) 192, 348
HgaI GACGC 3 cut(s) 145, 352, 397
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 3 cut(s) 156, 162, 168
Hin1II CATG 2 cut(s) 335, 566
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 160, 166
HindII GTYRAC 2 cut(s) 160, 166
HinfI GANTC 3 cut(s) 195, 248, 446
HpaII CCGG 2 cut(s) 192, 348
HphI GGTGA 2 cut(s) 274, 362
Hpy166II GTNNAC 4 cut(s) 48, 160, 166, 533
Hpy188I TCNGA 3 cut(s) 214, 271, 318
Hpy188III TCNNGA 3 cut(s) 192, 338, 563
Hpy8I GTNNAC 4 cut(s) 48, 160, 166, 533
Hpy99I CGWCG 6 cut(s) 161, 164, 167, 170, 173, 525
HpyAV CCTTC 3 cut(s) 104, 146, 493
HpyCH4IV ACGT 2 cut(s) 162, 168
HpyCH4V TGCA 2 cut(s) 331, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 467
HpyF3I CTNAG 1 cut(s) 432
HpySE526I ACGT 2 cut(s) 162, 168
Hsp92I GRCGYC 3 cut(s) 156, 162, 168
Hsp92II CATG 2 cut(s) 335, 566
HspAI GCGC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 191
Kzo9I GATC 2 cut(s) 38, 487
LmnI GCTCC 2 cut(s) 194, 340
LpnPI CCDG 6 cut(s) 205, 286, 313, 351, 361, 504
LweI GCATC 3 cut(s) 95, 370, 418
MaeII ACGT 2 cut(s) 162, 168
MaeIII GTNAC 2 cut(s) 364, 409
MalI GATC 2 cut(s) 40, 489
MbiI CCGCTC 1 cut(s) 267
MboI GATC 2 cut(s) 38, 487
MboII GAAGA 3 cut(s) 81, 84, 161
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 2 cut(s) 191, 535
MlyI GAGTC 1 cut(s) 455
MroI TCCGGA 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 192, 348
MspR9I CCNGG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 2 cut(s) 83, 467
NdeII GATC 2 cut(s) 38, 487
NlaIII CATG 2 cut(s) 335, 566
NlaIV GGNNCC 1 cut(s) 489
NmuCI GTSAC 2 cut(s) 364, 409
NspI RCATGY 1 cut(s) 335
NspV TTCGAA 1 cut(s) 358
PaeI GCATGC 1 cut(s) 335
PagI TCATGA 1 cut(s) 562
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 2 cut(s) 195, 248
PkrI GCNGC 2 cut(s) 66, 106
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
Psp124BI GAGCTC 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 1 cut(s) 345
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 1 cut(s) 487
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
SacI GAGCTC 1 cut(s) 191
SalI GTCGAC 2 cut(s) 158, 164
SatI GCNGC 2 cut(s) 65, 105
Sau3AI GATC 2 cut(s) 38, 487
Sau96I GGNCC 1 cut(s) 345
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 2 cut(s) 191, 535
SetI ASST 9 cut(s) 55, 84, 165, 171, 191, 287, 357, 472, 529
SfaNI GCATC 3 cut(s) 95, 370, 418
SfuI TTCGAA 1 cut(s) 358
SgrDI CGTCGACG 2 cut(s) 158, 164
SinI GGWCC 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
SphI GCATGC 1 cut(s) 335
SsiI CCGC 5 cut(s) 64, 104, 107, 115, 265
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 78
TaiI ACGT 2 cut(s) 165, 171
TaqI TCGA 4 cut(s) 159, 165, 358, 523
TatI WGTACW 1 cut(s) 493
TauI GCSGC 2 cut(s) 67, 107
TfiI GAWTC 2 cut(s) 195, 248
TscAI CASTG 2 cut(s) 189, 463
TseFI GTSAC 2 cut(s) 364, 409
Tsp45I GTSAC 2 cut(s) 364, 409
TspDTI ATGAA 2 cut(s) 338, 542
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 2 cut(s) 189, 463
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 1 cut(s) 345
XceI RCATGY 1 cut(s) 335
XmiI GTMKAC 2 cut(s) 159, 165
ZraI GACGTC 2 cut(s) 163, 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.