pycom215g00110

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000215
OG0016005
Physical Location & Seq
Forward (+)
233261 .. 234945
1685 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom215g00110.2

Sequence Viewer

Length: 447 bp
ATGGCGTCATTAAGTGTTAATGGTGCTTTATTAAGCATCGAAAACAAACGCTACACATGTTATTGTCCCAGGAGCGAGTGGTGGAGGAGCAGCAGCAGCAGCTCCAGACGATATTCAGTTACGCCAATAATAAGGTGCTCAGCTGATACTGATAGTATGGCTACTCAGAAATTGGGTATCAAAGTCGACAGGAATCCTCCCGAGTCCAGGCTCACCCAACTGGGCGTCAGAAATTGGCCCAAGTGGGGTTGCCCTCCAAGCAAGTTTCCATGGACATACAGTAACAAAGAGACTTGCTATCTGCTACAAGGCAAAGTGAAGGTTACCCCTGATGGATCCAATGAGTCGGTTGAGATTGGTGCTGGTGATTTGGTGGAATTTCCAAAGGGAATGAGCTGCACTTGGGATGTATCAGTGGCTGTAGACAAGCACTATAACTTCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

149

Amino Acids

16.61

Weight (kDa)

8.93

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016005)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10300
fragaria_vesca FvH4_2g00100 FvH4_2g00100
malus_domestica MD00G1089100.v1.1
prunus_persica Prupe.8G000800_v2.0.a1
pyrus_communis pycom215g00110
rosa_chinensis RchiOBHm_Chr6g0249351
rosa_laevigata RLG00000015589
rosa_roxburghii Rroxscaffold_7G00217820
rosa_rugosa Rorug05G0491000.1
rosa_samantha Rh6AG001400 Rh6BG008800 Rh6CG000500 Rh6DG000400
rosa_wichuraiana Rw6G000200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 186, 423
AclWI GGATC 2 cut(s) 330, 343
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 424
AcyI GRCGYC 2 cut(s) 5, 225
AfiI CCNNNNNNNGG 2 cut(s) 207, 245
AflIII ACRYGT 1 cut(s) 56
AjnI CCWGG 2 cut(s) 68, 206
AluBI AGCT 4 cut(s) 102, 143, 396, 444
AluI AGCT 4 cut(s) 102, 143, 396, 444
Alw21I GWGCWC 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 2 cut(s) 330, 343
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 1 cut(s) 236
ApeKI GCWGC 5 cut(s) 90, 93, 96, 99, 396
ApoI RAATTY 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 237
AsuHPI GGTGA 2 cut(s) 205, 377
AvaI CYCGRG 1 cut(s) 200
BamHI GGATCC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 5 cut(s) 102, 105, 108, 111, 383
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 2 cut(s) 70, 208
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 1 cut(s) 445
BfmI CTRYAG 1 cut(s) 420
BisI GCNGC 5 cut(s) 91, 94, 97, 100, 397
BlpI GCTNAGC 1 cut(s) 139
BlsI GCNGC 5 cut(s) 92, 95, 98, 101, 398
Bme1390I CCNGG 2 cut(s) 70, 208
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 237
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 2 cut(s) 70, 208
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 45
BmuI ACTGGG 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 88
Bpu1102I GCTNAGC 1 cut(s) 139
BsaHI GRCGYC 2 cut(s) 5, 225
BsaJI CCNNGG 2 cut(s) 68, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 245
Bse1I ACTGG 1 cut(s) 225
BseBI CCWGG 2 cut(s) 70, 208
BseDI CCNNGG 2 cut(s) 68, 269
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 2 cut(s) 207, 245
BseMII CTCAG 2 cut(s) 153, 179
BseNI ACTGG 1 cut(s) 225
BseRI GAGGAG 1 cut(s) 100
BseXI GCAGC 5 cut(s) 102, 105, 108, 111, 383
BsgI GTGCAG 1 cut(s) 382
BshFI GGCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 140
BsiHKCI CYCGRG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 207, 245
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 51
BsnI GGCC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 140
Bsp143I GATC 1 cut(s) 335
Bsp1720I GCTNAGC 1 cut(s) 139
Bsp19I CCATGG 1 cut(s) 269
BspANI GGCC 1 cut(s) 238
BspCNI CTCAG 2 cut(s) 152, 178
BspLI GGNNCC 1 cut(s) 337
BspPI GGATC 2 cut(s) 330, 343
BsrI ACTGG 1 cut(s) 225
BssECI CCNNGG 2 cut(s) 68, 269
BssMI GATC 1 cut(s) 335
BssNI GRCGYC 2 cut(s) 5, 225
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 70, 208
Bst4CI ACNGT 1 cut(s) 281
BstACI GRCGYC 2 cut(s) 5, 225
BstDEI CTNAG 2 cut(s) 139, 165
BstDSI CCRYGG 1 cut(s) 269
BstEII GGTNACC 1 cut(s) 322
BstF5I GGATG 1 cut(s) 412
BstKTI GATC 1 cut(s) 338
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 1 cut(s) 335
BstMWI GCNNNNNNNGC 3 cut(s) 96, 99, 258
BstNI CCWGG 2 cut(s) 70, 208
BstNSI RCATGY 1 cut(s) 60
BstPI GGTNACC 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 68, 206
BstSFI CTRYAG 1 cut(s) 420
BstV1I GCAGC 5 cut(s) 102, 105, 108, 111, 383
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuRI GGCC 1 cut(s) 238
BtgI CCRYGG 1 cut(s) 269
BtsCI GGATG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 420
CaiI CAGNNNCTG 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 237
CseI GACGC 1 cut(s) 214
CviAII CATG 2 cut(s) 57, 270
CviJI RGCY 8 cut(s) 102, 143, 161, 211, 238, 396, 419, 444
CviKI_1 RGCY 8 cut(s) 102, 143, 161, 211, 238, 396, 419, 444
DdeI CTNAG 2 cut(s) 139, 165
DpnI GATC 1 cut(s) 337
DpnII GATC 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 424
Eco88I CYCGRG 1 cut(s) 200
Eco91I GGTNACC 1 cut(s) 322
EcoO65I GGTNACC 1 cut(s) 322
EcoRII CCWGG 2 cut(s) 68, 206
EcoT14I CCWWGG 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 2 cut(s) 60, 273
FaiI YATR 5 cut(s) 58, 158, 271, 277, 435
FaqI GGGAC 1 cut(s) 51
FatI CATG 2 cut(s) 56, 269
FblI GTMKAC 2 cut(s) 186, 423
Fnu4HI GCNGC 5 cut(s) 91, 94, 97, 100, 397
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 5 cut(s) 91, 94, 97, 100, 397
FspBI CTAG 1 cut(s) 445
GluI GCNGC 5 cut(s) 91, 94, 97, 100, 397
GsuI CTGGAG 1 cut(s) 88
HaeIII GGCC 1 cut(s) 238
HgaI GACGC 1 cut(s) 214
Hin1I GRCGYC 2 cut(s) 5, 225
Hin1II CATG 2 cut(s) 60, 273
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 3 cut(s) 193, 203, 344
HphI GGTGA 2 cut(s) 205, 377
Hpy166II GTNNAC 2 cut(s) 187, 424
Hpy188I TCNGA 2 cut(s) 168, 230
Hpy188III TCNNGA 2 cut(s) 105, 200
Hpy8I GTNNAC 2 cut(s) 187, 424
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 1 cut(s) 399
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 99, 258
HpyF3I CTNAG 2 cut(s) 139, 165
Hsp92I GRCGYC 2 cut(s) 5, 225
Hsp92II CATG 2 cut(s) 60, 273
Kzo9I GATC 1 cut(s) 335
LmnI GCTCC 3 cut(s) 72, 87, 107
LpnPI CCDG 9 cut(s) 55, 82, 118, 175, 193, 206, 220, 342, 348
Lsp1109I GCAGC 5 cut(s) 102, 105, 108, 111, 383
LweI GCATC 1 cut(s) 45
MaeI CTAG 1 cut(s) 445
MaeIII GTNAC 3 cut(s) 118, 281, 322
MalI GATC 1 cut(s) 337
MboI GATC 1 cut(s) 335
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 3 cut(s) 170, 232, 377
MlyI GAGTC 2 cut(s) 212, 353
MnlI CCTC 3 cut(s) 78, 207, 264
MseI TTAA 3 cut(s) 11, 18, 32
MspA1I CMGCKG 1 cut(s) 143
MspR9I CCNGG 2 cut(s) 70, 208
MvaI CCWGG 2 cut(s) 70, 208
MwoI GCNNNNNNNGC 3 cut(s) 96, 99, 258
NcoI CCATGG 1 cut(s) 269
NdeII GATC 1 cut(s) 335
NlaIII CATG 2 cut(s) 60, 273
NlaIV GGNNCC 1 cut(s) 337
NspI RCATGY 1 cut(s) 60
PciI ACATGT 1 cut(s) 56
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 5 cut(s) 92, 95, 98, 101, 398
PleI GAGTC 2 cut(s) 211, 352
PpsI GAGTC 2 cut(s) 211, 352
PscI ACATGT 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 68, 206
PspEI GGTNACC 1 cut(s) 322
PspGI CCWGG 2 cut(s) 68, 206
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 335
PvuII CAGCTG 1 cut(s) 143
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 3 cut(s) 11, 18, 32
SatI GCNGC 5 cut(s) 91, 94, 97, 100, 397
Sau3AI GATC 1 cut(s) 335
Sau96I GGNCC 1 cut(s) 237
SchI GAGTC 2 cut(s) 212, 353
ScrFI CCNGG 2 cut(s) 70, 208
SduI GDGCHC 1 cut(s) 140
SetI ASST 6 cut(s) 104, 137, 145, 324, 398, 446
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 1 cut(s) 420
Sse9I AATT 3 cut(s) 170, 232, 377
SspMI CTAG 1 cut(s) 445
StyD4I CCNGG 2 cut(s) 68, 206
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 2 cut(s) 39, 186
TasI AATT 3 cut(s) 170, 232, 377
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 3 cut(s) 11, 18, 32
Tru9I TTAA 3 cut(s) 11, 18, 32
TscAI CASTG 1 cut(s) 420
TseI GCWGC 5 cut(s) 90, 93, 96, 99, 396
TspRI CASTG 1 cut(s) 420
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 60
XmiI GTMKAC 2 cut(s) 186, 423
XspI CTAG 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.