Rh6CG000500

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0016005
Physical Location & Seq
Reverse (-)
179689 .. 193142
13454 bp
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UTR
Exon/CDS
Intron
Rh6CG000500.1

Sequence Viewer

Length: 420 bp
ATGGCATCACTGTGCGTTAATAATAGCTTTATATTAAACACCCAAACTCTACACAAACGCTCCTTTCTGCCTGCAACAACCAACAAAAGAAGAAGATCAGTGACCATAGCAGCAGAGTCATCTTCTTCAATGGCTACTGAAAAATTGGGTATCAAGATTGAAAAGAATCCTCCCGAGTCCACGCTCACCCAGCTCGGCGTCAGGAATTGGCCCAAGTGGAGTTGCCCTCCAAGCAAGTTTCCATGGACGTACAGTGACAAAGAGACTTGCTATCTGTTGGAAGGAAAGGTGACGGTTACTCCTGCTGGATCAAAAGAGTCGGTTGAGATTGGTGGTGGTGACTTGGTGGAATTTCCTAAAGGAATGAGCTGCACGTGGGATGTGTCAGTGGGGGTAGACAAGCACTATAACTTCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

139

Amino Acids

15.33

Weight (kDa)

8.93

Isoelectric Point (pI)

62.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 63 - 136 8.4e-29 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016005)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10300
fragaria_vesca FvH4_2g00100 FvH4_2g00100
malus_domestica MD00G1089100.v1.1
prunus_persica Prupe.8G000800_v2.0.a1
pyrus_communis pycom215g00110
rosa_chinensis RchiOBHm_Chr6g0249351
rosa_laevigata RLG00000015589
rosa_roxburghii Rroxscaffold_7G00217820
rosa_rugosa Rorug05G0491000.1
rosa_samantha Rh6AG001400 Rh6BG008800 Rh6CG000500 Rh6DG000400
rosa_wichuraiana Rw6G000200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 396
AclWI GGATC 1 cut(s) 316
AcsI RAATTY 1 cut(s) 350
AcvI CACGTG 1 cut(s) 375
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 251
AgsI TTSAA 2 cut(s) 129, 161
AluBI AGCT 3 cut(s) 27, 193, 369
AluI AGCT 3 cut(s) 27, 193, 369
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 2 cut(s) 110, 369
ApoI RAATTY 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 178, 301, 350
AvaI CYCGRG 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 375
BbvI GCAGC 2 cut(s) 122, 356
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 1 cut(s) 418
BisI GCNGC 2 cut(s) 111, 370
BlsI GCNGC 2 cut(s) 112, 371
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 210
BmsI GCATC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 211, 243
BsaAI YACGTR 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 242
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 283, 313
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 385
BseXI GCAGC 2 cut(s) 122, 356
BseYI CCCAGC 1 cut(s) 189
BsgI GTGCAG 1 cut(s) 355
BshFI GGCC 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 173
BsmAI GTCTC 1 cut(s) 257
BsnI GGCC 1 cut(s) 211
BsoBI CYCGRG 1 cut(s) 173
Bsp143I GATC 2 cut(s) 95, 308
Bsp19I CCATGG 1 cut(s) 242
BspANI GGCC 1 cut(s) 211
BspPI GGATC 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 2 cut(s) 95, 308
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 242
Bst4CI ACNGT 3 cut(s) 12, 254, 295
BstACI GRCGYC 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 72
BstDSI CCRYGG 1 cut(s) 242
BstF5I GGATG 1 cut(s) 385
BstKTI GATC 2 cut(s) 98, 311
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 2 cut(s) 95, 308
BstMWI GCNNNNNNNGC 2 cut(s) 190, 231
BstV1I GCAGC 2 cut(s) 122, 356
BsuRI GGCC 1 cut(s) 211
BtgI CCRYGG 1 cut(s) 242
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 4 cut(s) 8, 105, 259, 393
Cac8I GCNNGC 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 210
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 243
CviJI RGCY 6 cut(s) 27, 134, 193, 211, 369, 417
CviKI_1 RGCY 6 cut(s) 27, 134, 193, 211, 369, 417
CviQI GTAC 1 cut(s) 250
DpnI GATC 2 cut(s) 97, 310
DpnII GATC 2 cut(s) 95, 308
Eco130I CCWWGG 1 cut(s) 242
Eco72I CACGTG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 242
FaeI CATG 1 cut(s) 246
FaiI YATR 4 cut(s) 32, 107, 244, 408
FatI CATG 1 cut(s) 242
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 2 cut(s) 111, 370
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 2 cut(s) 111, 370
FspBI CTAG 1 cut(s) 418
GluI GCNGC 2 cut(s) 111, 370
GsaI CCCAGC 1 cut(s) 193
HaeIII GGCC 1 cut(s) 211
HgaI GACGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 1 cut(s) 246
HinfI GANTC 4 cut(s) 116, 166, 176, 317
HphI GGTGA 3 cut(s) 178, 301, 350
Hpy166II GTNNAC 2 cut(s) 180, 397
Hpy188III TCNNGA 3 cut(s) 154, 173, 202
Hpy8I GTNNAC 2 cut(s) 180, 397
HpyAV CCTTC 1 cut(s) 275
HpyCH4III ACNGT 3 cut(s) 12, 254, 295
HpyCH4IV ACGT 2 cut(s) 248, 374
HpyCH4V TGCA 2 cut(s) 74, 372
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 231
HpySE526I ACGT 2 cut(s) 248, 374
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 1 cut(s) 246
Kzo9I GATC 2 cut(s) 95, 308
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 5 cut(s) 84, 187, 203, 291, 315
Lsp1109I GCAGC 2 cut(s) 122, 356
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 418
MaeII ACGT 2 cut(s) 248, 374
MaeIII GTNAC 5 cut(s) 100, 254, 289, 295, 338
MalI GATC 2 cut(s) 97, 310
MboI GATC 2 cut(s) 95, 308
MboII GAAGA 4 cut(s) 102, 105, 114, 117
MluCI AATT 3 cut(s) 143, 205, 350
MlyI GAGTC 3 cut(s) 125, 185, 326
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 2 cut(s) 180, 237
MseI TTAA 2 cut(s) 18, 35
MslI CAYNNNNRTG 1 cut(s) 10
MwoI GCNNNNNNNGC 2 cut(s) 190, 231
NcoI CCATGG 1 cut(s) 242
NdeII GATC 2 cut(s) 95, 308
NlaIII CATG 1 cut(s) 246
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 4 cut(s) 100, 254, 289, 338
PfeI GAWTC 1 cut(s) 166
PkrI GCNGC 2 cut(s) 112, 371
PleI GAGTC 3 cut(s) 124, 184, 325
PmaCI CACGTG 1 cut(s) 375
PmlI CACGTG 1 cut(s) 375
PpsI GAGTC 3 cut(s) 124, 184, 325
Ppu21I YACGTR 1 cut(s) 375
PspCI CACGTG 1 cut(s) 375
PspFI CCCAGC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 210
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 10
SaqAI TTAA 2 cut(s) 18, 35
SatI GCNGC 2 cut(s) 111, 370
Sau3AI GATC 2 cut(s) 95, 308
Sau96I GGNCC 1 cut(s) 210
SchI GAGTC 3 cut(s) 125, 185, 326
SetI ASST 6 cut(s) 29, 195, 251, 291, 371, 377
SfaNI GCATC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 10
Sse9I AATT 3 cut(s) 143, 205, 350
SspMI CTAG 1 cut(s) 418
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 3 cut(s) 12, 254, 295
TaiI ACGT 2 cut(s) 251, 377
TasI AATT 3 cut(s) 143, 205, 350
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 2 cut(s) 18, 35
Tru9I TTAA 2 cut(s) 18, 35
TscAI CASTG 4 cut(s) 15, 105, 259, 393
TseFI GTSAC 4 cut(s) 100, 254, 289, 338
TseI GCWGC 2 cut(s) 110, 369
Tsp45I GTSAC 4 cut(s) 100, 254, 289, 338
TspRI CASTG 4 cut(s) 15, 105, 259, 393
XapI RAATTY 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 396
XspI CTAG 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.