pycom420g00960

Adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0009985
Physical Location & Seq
Reverse (-)
1283070 .. 1285291
2222 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1839 bp
ATGCCACAGTACACAATCGTTCACCAACCCACACTCGACCACGTGTCACGTAGCTCGGACCCTTATCTGCAATCGATGAACGATCATCCAAAGAGAACCACGACCCACAAGGGCTGGGTCCCGCATCATCTTCCGTCTACTACGCCAAATTTCGTTTCGCTCTCATTTACCAGATCCACCAACTCCTCCCACCACGAAATCACTACCGTCCATCTCACGCTCCCTCCCTATTTATACCCCCTCACTCTCCCTCCCTATTTGTCCCCCTCACTCTCTTGCTCCACCCTCGCTTTAATTCCTCCCTCCCAAAAACAAAACCCTACTTTGGGCAGTGTGAGCGAGATTACCACCGGTCTCCACCGCCAACCCCACCCACCAACAATGGCTCTCTCCGTCGAGAAAAGTAGCAGCGGCCGCGAGTACAAGGTCAAGGACATGTCCCAGGCCGACTTCGGCCGCCTCGAGATCGAGCTCGCTGAGGTTGAAATGCCTGGCCTCATCTCATGCCGCACCGAATTCGGACCCTCCCAACCCTTCAAGGGGGCCCGCATCACCGGTTCCCTCCACATGACCATCCAAACCGCTGTCCTCATTGAAACCCTCACCGCCCTCGGCGCCGAGGTCCGCTGGTGCTCCTGCAACATTTTCTCCACCCAGGACCACGCCGCCGCCGCCATCGCCCGCGATAGCGCCGCCGTCTTCGCCTGGAAGGGCGAGACCCTCCAGGAGTACTGGTGGTGCACCGAGCGCGCCCTCGACTGGGGCCCCGGTGGCGGACCCGATCTGATCGTCGACGACGGCGGTGATGCCACCCTCTTGATCCACGAGGGAGTCAAGGCTGAGGAGGAGTTCGAGAAGTCCGGTACCCTCCCTGACCCGTCTTCCACCGATAACCAGGAGTTCCAGCTGGTTCTGACCATCATCAGAGATGGCTTGAAGACCGACCCCAAGAGGTACCACAAGATGAAGGACAGGTTGGTCGGAGTTTCGGAGGAGACCACCACCGGCGTCAAGAGGTTGTATCAGATGCAGGCTAGCAGTACTCTGTTGTTCCCCGCCATTAATGTCAATGACTCTGTTACAAAAAGCAAGTTTGACAACTTGTACGGATGCCGTCACTCTCTCCCTGATGGTTTGATGAGGGCCACTGATGTCATGATTGCCGGAAAGGTTTCCGTTGTTTGCGGATACGGAGATGTTGGAAAGGGTTGCGCTGCTGCCCTCAAGCAAGCAGGATCTCGTGTGATTGTTACTGAGATCGATCCAATCTGTGCCCTCCAGGCTCTTATGGAAGGCCTTCAGGTTCTTCCTCTTGAGGATGTTGTCTCCGAGGCGGATATCTTTGTTACCACCACCGGTAACAAGGACATCATCATGGTTGACCACATGAGGAAGATGAAGAACAATGCCATTGTTTGCAACATTGGTCACTTTGACAATGAGATTGACATGCACGGTCTTGAGACATACGCCGGAGTGAAGCGCATCACCATTAAGCCTCAAACTGACAGGTGGGTCTTCCCAGAGACCAACACTGGCATCATTGTGTTGGCTGAGGGCCGTCTCATGAACTTGGGATGTGCCACTGGACACCCCAGCTTTGTGATGTCCTGCTCTTTCACCAACCAAGTGATTGCTCAGCTTGAGTTGTGGAACGAAAGGAAGTCTGGCAAGTACGAGAAGAAGGTGTATGTCTTGCCTAAGCACCTTGACGAGAAGGTTGCTGCTCTTCATCTTGGAAAGCTTGGAGCTAAGCTCACCAAACTCAGCAAGGAACAGGCTGACTACATCAGTGTGCCGGTTGAGGGTCCATACAAGCCAGCGCACTACAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

613

Amino Acids

67.48

Weight (kDa)

6.29

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 140 - 611 2.7e-145 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 367 - 530 1.2e-82 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
2-Hacid_dh_C PF02826 386 - 476 1.4e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 977, 1514
Acc65I GGTACC 2 cut(s) 863, 954
AccB1I GGYRCC 3 cut(s) 614, 863, 954
AccI GTMKAC 2 cut(s) 137, 792
AccII CGCG 3 cut(s) 417, 684, 750
AclWI GGATC 4 cut(s) 168, 814, 1243, 1256
AcoI YGGCCR 2 cut(s) 412, 454
AcsI RAATTY 2 cut(s) 148, 515
AcuI CTGAAG 1 cut(s) 1283
AcvI CACGTG 1 cut(s) 43
AcyI GRCGYC 2 cut(s) 615, 1008
AfaI GTAC 9 cut(s) 11, 422, 731, 865, 956, 1042, 1106, 1676, 1835
AfiI CCNNNNNNNGG 8 cut(s) 325, 326, 539, 540, 759, 760, 773, 1805
AflIII ACRYGT 2 cut(s) 42, 435
AgeI ACCGGT 3 cut(s) 350, 554, 1355
AgsI TTSAA 4 cut(s) 485, 538, 596, 937
AhdI GACNNNNNGTC 1 cut(s) 43
AjnI CCWGG 7 cut(s) 441, 490, 654, 704, 723, 894, 1278
AjuI GAANNNNNNNTTGG 4 cut(s) 139, 171, 959, 991
AluBI AGCT 8 cut(s) 54, 472, 907, 1599, 1642, 1744, 1751, 1756
AluI AGCT 8 cut(s) 54, 472, 907, 1599, 1642, 1744, 1751, 1756
Alw21I GWGCWC 3 cut(s) 474, 635, 743
Alw26I GTCTC 7 cut(s) 359, 710, 989, 1330, 1457, 1520, 1568
Alw44I GTGCAC 1 cut(s) 739
AlwI GGATC 4 cut(s) 168, 814, 1243, 1256
AlwNI CAGNNNCTG 1 cut(s) 1836
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 9 cut(s) 412, 444, 454, 493, 543, 763, 1143, 1294, 1558
ApaI GGGCCC 2 cut(s) 547, 767
ApaLI GTGCAC 1 cut(s) 739
ApeKI GCWGC 4 cut(s) 408, 1214, 1217, 1724
ApoI RAATTY 2 cut(s) 148, 515
AseI ATTAAT 1 cut(s) 1062
AsiGI ACCGGT 3 cut(s) 350, 554, 1355
Asp700I GAANNNNTTC 2 cut(s) 1171, 1296
Asp718I GGTACC 2 cut(s) 863, 954
AspLEI GCGC 7 cut(s) 617, 692, 750, 752, 1214, 1485, 1825
AsuC2I CCSGG 1 cut(s) 768
AsuHPI GGTGA 7 cut(s) 14, 544, 595, 815, 1480, 1612, 1750
AsuNHI GCTAGC 1 cut(s) 1034
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 7 cut(s) 58, 118, 521, 622, 658, 776, 1808
BaeGI GKGCMC 4 cut(s) 547, 743, 767, 1276
BanI GGYRCC 3 cut(s) 614, 863, 954
BanII GRGCYC 3 cut(s) 474, 547, 767
BauI CACGAG 2 cut(s) 824, 1239
BbrPI CACGTG 1 cut(s) 43
BbsI GAAGAC 4 cut(s) 691, 873, 944, 1510
Bbv12I GWGCWC 3 cut(s) 474, 635, 743
BbvCI CCTCAGC 3 cut(s) 477, 840, 1554
BbvI GCAGC 4 cut(s) 420, 1201, 1204, 1711
BccI CCATC 6 cut(s) 219, 581, 683, 923, 926, 1124
BceAI ACGGC 4 cut(s) 680, 814, 1098, 1545
BcgI CGANNNNNNTGC 4 cut(s) 499, 533, 1703, 1737
BciT130I CCWGG 7 cut(s) 443, 492, 656, 706, 725, 896, 1280
BciVI GTATCC 1 cut(s) 1181
BcnI CCSGG 1 cut(s) 768
BcoDI GTCTC 7 cut(s) 359, 710, 989, 1330, 1457, 1520, 1568
BfaI CTAG 1 cut(s) 1035
BfmI CTRYAG 1 cut(s) 1828
BfoI RGCGCY 2 cut(s) 618, 693
BfuI GTATCC 1 cut(s) 1181
BglI GCCNNNNNGGC 2 cut(s) 771, 1280
BlpI GCTNAGC 2 cut(s) 1638, 1752
BmcAI AGTACT 2 cut(s) 731, 1042
Bme1390I CCNGG 8 cut(s) 443, 492, 656, 706, 725, 768, 896, 1280
Bme18I GGWCC 7 cut(s) 58, 118, 521, 622, 658, 776, 1808
BmeRI GACNNNNNGTC 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 461
BmrFI CCNGG 8 cut(s) 443, 492, 656, 706, 725, 768, 896, 1280
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 7 cut(s) 133, 558, 796, 1017, 1100, 1494, 1548
BmtI GCTAGC 1 cut(s) 1038
BmuI ACTGGG 1 cut(s) 769
BpiI GAAGAC 4 cut(s) 691, 873, 944, 1510
BplI GAGNNNNNCTC 2 cut(s) 1740, 1772
BpmI CTGGAG 2 cut(s) 707, 1262
Bpu10I CCTNAGC 4 cut(s) 477, 840, 1554, 1701
Bpu1102I GCTNAGC 2 cut(s) 1638, 1752
BpuEI CTTGAG 4 cut(s) 1208, 1334, 1481, 1664
BpuMI CCSGG 1 cut(s) 768
Bsa29I ATCGAT 2 cut(s) 74, 1260
BsaAI YACGTR 2 cut(s) 43, 50
BsaHI GRCGYC 2 cut(s) 615, 1008
BsaI GGTCTC 4 cut(s) 359, 710, 989, 1520
BsaJI CCNNGG 6 cut(s) 441, 610, 618, 654, 766, 1329
BsaWI WCCGGW 4 cut(s) 350, 554, 860, 1355
BsaXI ACNNNNNCTCC 8 cut(s) 208, 235, 238, 265, 632, 662, 719, 749
Bsc4I CCNNNNNNNGG 8 cut(s) 325, 326, 539, 540, 759, 760, 773, 1805
Bse118I RCCGGY 5 cut(s) 350, 554, 1004, 1355, 1798
Bse1I ACTGG 4 cut(s) 737, 764, 1540, 1591
BseBI CCWGG 7 cut(s) 443, 492, 656, 706, 725, 896, 1280
BseCI ATCGAT 2 cut(s) 74, 1260
BseDI CCNNGG 6 cut(s) 441, 610, 618, 654, 766, 1329
BseGI GGATG 5 cut(s) 85, 573, 1115, 1324, 1583
BseLI CCNNNNNNNGG 8 cut(s) 325, 326, 539, 540, 759, 760, 773, 1805
BseMII CTCAG 6 cut(s) 468, 831, 1245, 1545, 1652, 1780
BseNI ACTGG 4 cut(s) 737, 764, 1540, 1591
BsePI GCGCGC 1 cut(s) 748
BseRI GAGGAG 4 cut(s) 175, 857, 860, 1007
BseSI GKGCMC 4 cut(s) 547, 743, 767, 1276
BseX3I CGGCCG 2 cut(s) 412, 454
BseXI GCAGC 4 cut(s) 420, 1201, 1204, 1711
BseYI CCCAGC 2 cut(s) 114, 1595
Bsh1236I CGCG 3 cut(s) 417, 684, 750
Bsh1285I CGRYCG 2 cut(s) 415, 457
BshFI GGCC 9 cut(s) 414, 446, 456, 495, 545, 765, 1145, 1296, 1560
BshNI GGYRCC 3 cut(s) 614, 863, 954
BshTI ACCGGT 3 cut(s) 350, 554, 1355
BshVI ATCGAT 2 cut(s) 74, 1260
BsiEI CGRYCG 2 cut(s) 415, 457
BsiHKAI GWGCWC 3 cut(s) 474, 635, 743
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 9 cut(s) 351, 555, 768, 861, 1005, 1164, 1356, 1473, 1799
BslFI GGGAC 3 cut(s) 104, 247, 424
BslI CCNNNNNNNGG 8 cut(s) 325, 326, 539, 540, 759, 760, 773, 1805
BsmAI GTCTC 7 cut(s) 359, 710, 989, 1330, 1457, 1520, 1568
BsmBI CGTCTC 1 cut(s) 1568
BsmFI GGGAC 3 cut(s) 104, 247, 424
BsnI GGCC 9 cut(s) 414, 446, 456, 495, 545, 765, 1145, 1296, 1560
Bso31I GGTCTC 4 cut(s) 359, 710, 989, 1520
BsoBI CYCGRG 1 cut(s) 461
Bsp120I GGGCCC 2 cut(s) 543, 763
Bsp1286I GDGCHC 6 cut(s) 474, 547, 635, 743, 767, 1276
Bsp143I GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
Bsp1720I GCTNAGC 2 cut(s) 1638, 1752
BspANI GGCC 9 cut(s) 414, 446, 456, 495, 545, 765, 1145, 1296, 1560
BspCNI CTCAG 6 cut(s) 469, 832, 1246, 1546, 1651, 1779
BspDI ATCGAT 2 cut(s) 74, 1260
BspFNI CGCG 3 cut(s) 417, 684, 750
BspHI TCATGA 2 cut(s) 1155, 1566
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 4 cut(s) 168, 814, 1243, 1256
BspQI GCTCTTC 1 cut(s) 1734
BspT107I GGYRCC 3 cut(s) 614, 863, 954
BspTNI GGTCTC 4 cut(s) 359, 710, 989, 1520
BsrFI RCCGGY 5 cut(s) 350, 554, 1004, 1355, 1798
BsrI ACTGG 4 cut(s) 737, 764, 1540, 1591
BssAI RCCGGY 5 cut(s) 350, 554, 1004, 1355, 1798
BssECI CCNNGG 6 cut(s) 441, 610, 618, 654, 766, 1329
BssHII GCGCGC 1 cut(s) 748
BssMI GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
BssNI GRCGYC 2 cut(s) 615, 1008
BssSI CACGAG 2 cut(s) 824, 1239
Bst2BI CACGAG 2 cut(s) 824, 1239
Bst2UI CCWGG 7 cut(s) 443, 492, 656, 706, 725, 896, 1280
Bst4CI ACNGT 3 cut(s) 9, 208, 1457
Bst6I CTCTTC 1 cut(s) 1734
BstACI GRCGYC 2 cut(s) 615, 1008
BstBAI YACGTR 2 cut(s) 43, 50
BstC8I GCNNGC 8 cut(s) 474, 547, 682, 750, 1032, 1036, 1230, 1821
BstDEI CTNAG 8 cut(s) 477, 840, 1254, 1554, 1638, 1701, 1752, 1766
BstF5I GGATG 5 cut(s) 85, 573, 1115, 1324, 1583
BstFNI CGCG 3 cut(s) 417, 684, 750
BstH2I RGCGCY 2 cut(s) 618, 693
BstHHI GCGC 7 cut(s) 617, 692, 750, 752, 1214, 1485, 1825
BstKTI GATC 9 cut(s) 85, 176, 468, 784, 789, 822, 1238, 1260, 1264
BstMAI GTCTC 7 cut(s) 359, 710, 989, 1330, 1457, 1520, 1568
BstMBI GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
BstMCI CGRYCG 2 cut(s) 415, 457
BstMWI GCNNNNNNNGC 9 cut(s) 336, 414, 614, 671, 677, 701, 747, 771, 1280
BstNI CCWGG 7 cut(s) 443, 492, 656, 706, 725, 896, 1280
BstNSI RCATGY 2 cut(s) 439, 1453
BstSCI CCNGG 8 cut(s) 441, 490, 654, 704, 723, 766, 894, 1278
BstSFI CTRYAG 1 cut(s) 1828
BstSLI GKGCMC 4 cut(s) 547, 743, 767, 1276
BstUI CGCG 3 cut(s) 417, 684, 750
BstV1I GCAGC 4 cut(s) 420, 1201, 1204, 1711
BstV2I GAAGAC 4 cut(s) 691, 873, 944, 1510
BstX2I RGATCY 2 cut(s) 173, 1235
BstYI RGATCY 2 cut(s) 173, 1235
BstZI CGGCCG 2 cut(s) 412, 454
Bsu15I ATCGAT 2 cut(s) 74, 1260
BsuI GTATCC 1 cut(s) 1181
BsuRI GGCC 9 cut(s) 414, 446, 456, 495, 545, 765, 1145, 1296, 1560
BsuTUI ATCGAT 2 cut(s) 74, 1260
BtgZI GCGATG 1 cut(s) 661
BtsCI GGATG 5 cut(s) 85, 573, 1115, 1324, 1583
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 5 cut(s) 337, 1146, 1533, 1584, 1798
Cac8I GCNNGC 8 cut(s) 474, 547, 682, 750, 1032, 1036, 1230, 1821
CaiI CAGNNNCTG 1 cut(s) 1836
CciI TCATGA 2 cut(s) 1155, 1566
CciNI GCGGCCGC 1 cut(s) 412
CfoI GCGC 7 cut(s) 617, 692, 750, 752, 1214, 1485, 1825
Cfr10I RCCGGY 5 cut(s) 350, 554, 1004, 1355, 1798
ClaI ATCGAT 2 cut(s) 74, 1260
CseI GACGC 1 cut(s) 997
Csp6I GTAC 9 cut(s) 10, 421, 730, 864, 955, 1041, 1105, 1675, 1834
CspAI ACCGGT 3 cut(s) 350, 554, 1355
CviAII CATG 8 cut(s) 436, 504, 568, 1156, 1375, 1387, 1450, 1567
CviQI GTAC 9 cut(s) 10, 421, 730, 864, 955, 1041, 1105, 1675, 1834
DdeI CTNAG 8 cut(s) 477, 840, 1254, 1554, 1638, 1701, 1752, 1766
DinI GGCGCC 1 cut(s) 616
DpnI GATC 9 cut(s) 84, 175, 467, 783, 788, 821, 1237, 1259, 1263
DpnII GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
DrdI GACNNNNNNGTC 2 cut(s) 977, 1514
DriI GACNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 2 cut(s) 977, 1514
EaeI YGGCCR 2 cut(s) 412, 454
EagI CGGCCG 2 cut(s) 412, 454
Eam1104I CTCTTC 1 cut(s) 1734
Eam1105I GACNNNNNGTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 1734
EciI GGCGGA 2 cut(s) 789, 1349
Ecl136II GAGCTC 1 cut(s) 472
EclXI CGGCCG 2 cut(s) 412, 454
Eco147I AGGCCT 1 cut(s) 1296
Eco24I GRGCYC 3 cut(s) 474, 547, 767
Eco31I GGTCTC 4 cut(s) 359, 710, 989, 1520
Eco32I GATATC 1 cut(s) 1339
Eco47I GGWCC 7 cut(s) 58, 118, 521, 622, 658, 776, 1808
Eco52I CGGCCG 2 cut(s) 412, 454
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 1283
Eco72I CACGTG 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 4 cut(s) 118, 543, 763, 764
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 7 cut(s) 441, 490, 654, 704, 723, 894, 1278
EcoRV GATATC 1 cut(s) 1339
EcoT38I GRGCYC 3 cut(s) 474, 547, 767
EgeI GGCGCC 1 cut(s) 616
EheI GGCGCC 1 cut(s) 616
Esp3I CGTCTC 1 cut(s) 1568
FaeI CATG 8 cut(s) 439, 507, 571, 1159, 1378, 1390, 1453, 1570
FaqI GGGAC 3 cut(s) 104, 247, 424
FatI CATG 8 cut(s) 435, 503, 567, 1155, 1374, 1386, 1449, 1566
FauI CCCGC 4 cut(s) 129, 554, 689, 1063
FblI GTMKAC 2 cut(s) 137, 792
FokI GGATG 5 cut(s) 72, 560, 1122, 1331, 1590
FriOI GRGCYC 3 cut(s) 474, 547, 767
FspBI CTAG 1 cut(s) 1035
GlaI GCGC 7 cut(s) 616, 691, 749, 751, 1213, 1484, 1824
GsaI CCCAGC 2 cut(s) 118, 1599
GsuI CTGGAG 2 cut(s) 707, 1262
HaeII RGCGCY 2 cut(s) 618, 693
HaeIII GGCC 9 cut(s) 414, 446, 456, 495, 545, 765, 1145, 1296, 1560
HapII CCGG 9 cut(s) 351, 555, 768, 861, 1005, 1164, 1356, 1473, 1799
HgaI GACGC 1 cut(s) 997
HhaI GCGC 7 cut(s) 617, 692, 750, 752, 1214, 1485, 1825
Hin1I GRCGYC 2 cut(s) 615, 1008
Hin1II CATG 8 cut(s) 439, 507, 571, 1159, 1378, 1390, 1453, 1570
Hin6I GCGC 7 cut(s) 615, 690, 748, 750, 1212, 1483, 1823
HinP1I GCGC 7 cut(s) 615, 690, 748, 750, 1212, 1483, 1823
HincII GTYRAC 2 cut(s) 793, 1381
HindII GTYRAC 2 cut(s) 793, 1381
HindIII AAGCTT 1 cut(s) 1742
HinfI GANTC 2 cut(s) 831, 1073
HpaII CCGG 9 cut(s) 351, 555, 768, 861, 1005, 1164, 1356, 1473, 1799
HphI GGTGA 7 cut(s) 14, 544, 595, 815, 1480, 1612, 1750
Hpy166II GTNNAC 6 cut(s) 12, 22, 138, 741, 793, 1381
Hpy188I TCNGA 9 cut(s) 58, 521, 786, 915, 926, 983, 991, 1026, 1330
Hpy188III TCNNGA 9 cut(s) 397, 463, 817, 853, 1012, 1156, 1313, 1460, 1567
Hpy8I GTNNAC 6 cut(s) 12, 22, 138, 741, 793, 1381
Hpy99I CGWCG 4 cut(s) 398, 794, 797, 800
HpyAV CCTTC 7 cut(s) 544, 703, 961, 1286, 1307, 1678, 1711
HpyCH4III ACNGT 3 cut(s) 9, 208, 1457
HpyCH4IV ACGT 2 cut(s) 42, 49
HpyCH4V TGCA 6 cut(s) 70, 639, 741, 1030, 1419, 1453
HpyF10VI GCNNNNNNNGC 9 cut(s) 336, 414, 614, 671, 677, 701, 747, 771, 1280
HpyF3I CTNAG 8 cut(s) 477, 840, 1254, 1554, 1638, 1701, 1752, 1766
HpySE526I ACGT 2 cut(s) 42, 49
Hsp92I GRCGYC 2 cut(s) 615, 1008
Hsp92II CATG 8 cut(s) 439, 507, 571, 1159, 1378, 1390, 1453, 1570
HspAI GCGC 7 cut(s) 615, 690, 748, 750, 1212, 1483, 1823
KasI GGCGCC 1 cut(s) 614
KflI GGGWCCC 1 cut(s) 118
KpnI GGTACC 2 cut(s) 867, 958
Kzo9I GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
LguI GCTCTTC 1 cut(s) 1734
LmnI GCTCC 4 cut(s) 225, 284, 638, 1748
Lsp1109I GCAGC 4 cut(s) 420, 1201, 1204, 1711
LweI GCATC 7 cut(s) 133, 558, 796, 1017, 1100, 1494, 1548
MaeI CTAG 1 cut(s) 1035
MaeII ACGT 2 cut(s) 42, 49
MaeIII GTNAC 7 cut(s) 45, 1078, 1115, 1249, 1345, 1358, 1427
MalI GATC 9 cut(s) 84, 175, 467, 783, 788, 821, 1237, 1259, 1263
MboI GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
MflI RGATCY 2 cut(s) 173, 1235
MhlI GDGCHC 6 cut(s) 474, 547, 635, 743, 767, 1276
MluCI AATT 3 cut(s) 148, 294, 515
Mly113I GGCGCC 1 cut(s) 615
MlyI GAGTC 2 cut(s) 840, 1067
MmeI TCCRAC 2 cut(s) 961, 1180
MroXI GAANNNNTTC 2 cut(s) 1171, 1296
MseI TTAA 3 cut(s) 293, 1062, 1494
MslI CAYNNNNRTG 3 cut(s) 1373, 1544, 1793
MspA1I CMGCKG 4 cut(s) 411, 584, 627, 907
MspI CCGG 9 cut(s) 351, 555, 768, 861, 1005, 1164, 1356, 1473, 1799
MspR9I CCNGG 8 cut(s) 443, 492, 656, 706, 725, 768, 896, 1280
MvaI CCWGG 7 cut(s) 443, 492, 656, 706, 725, 896, 1280
MvnI CGCG 3 cut(s) 417, 684, 750
MwoI GCNNNNNNNGC 9 cut(s) 336, 414, 614, 671, 677, 701, 747, 771, 1280
NarI GGCGCC 1 cut(s) 615
NciI CCSGG 1 cut(s) 768
NdeII GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
NheI GCTAGC 1 cut(s) 1034
NlaIII CATG 8 cut(s) 439, 507, 571, 1159, 1378, 1390, 1453, 1570
NmeAIII GCCGAG 2 cut(s) 591, 643
NmuCI GTSAC 3 cut(s) 45, 1115, 1427
NotI GCGGCCGC 1 cut(s) 412
NspI RCATGY 2 cut(s) 439, 1453
PaeR7I CTCGAG 1 cut(s) 461
PagI TCATGA 2 cut(s) 1155, 1566
PauI GCGCGC 1 cut(s) 748
PceI AGGCCT 1 cut(s) 1296
PciI ACATGT 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 1734
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PdmI GAANNNNTTC 2 cut(s) 1171, 1296
PfoI TCCNGGA 1 cut(s) 723
PinAI ACCGGT 3 cut(s) 350, 554, 1355
PleI GAGTC 2 cut(s) 839, 1067
PluTI GGCGCC 1 cut(s) 618
PmaCI CACGTG 1 cut(s) 43
PmlI CACGTG 1 cut(s) 43
PpsI GAGTC 2 cut(s) 839, 1067
Ppu21I YACGTR 2 cut(s) 43, 50
PpuMI RGGWCCY 1 cut(s) 118
PscI ACATGT 1 cut(s) 435
PshBI ATTAAT 1 cut(s) 1062
Psp124BI GAGCTC 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 118
Psp6I CCWGG 7 cut(s) 441, 490, 654, 704, 723, 894, 1278
PspCI CACGTG 1 cut(s) 43
PspFI CCCAGC 2 cut(s) 114, 1595
PspGI CCWGG 7 cut(s) 441, 490, 654, 704, 723, 894, 1278
PspOMI GGGCCC 2 cut(s) 543, 763
PspPPI RGGWCCY 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 1836
PsuI RGATCY 2 cut(s) 173, 1235
PteI GCGCGC 1 cut(s) 748
PvuII CAGCTG 1 cut(s) 907
RsaI GTAC 9 cut(s) 11, 422, 731, 865, 956, 1042, 1106, 1676, 1835
RsaNI GTAC 9 cut(s) 10, 421, 730, 864, 955, 1041, 1105, 1675, 1834
RseI CAYNNNNRTG 3 cut(s) 1373, 1544, 1793
SacI GAGCTC 1 cut(s) 474
SalI GTCGAC 1 cut(s) 791
SapI GCTCTTC 1 cut(s) 1734
SaqAI TTAA 3 cut(s) 293, 1062, 1494
Sau3AI GATC 9 cut(s) 82, 173, 465, 781, 786, 819, 1235, 1257, 1261
ScaI AGTACT 2 cut(s) 731, 1042
SchI GAGTC 2 cut(s) 840, 1067
ScrFI CCNGG 8 cut(s) 443, 492, 656, 706, 725, 768, 896, 1280
SduI GDGCHC 6 cut(s) 474, 547, 635, 743, 767, 1276
SfaNI GCATC 7 cut(s) 133, 558, 796, 1017, 1100, 1494, 1548
SfcI CTRYAG 1 cut(s) 1828
SfoI GGCGCC 1 cut(s) 616
Sfr274I CTCGAG 1 cut(s) 461
SgrAI CRCCGGYG 1 cut(s) 1004
SgrDI CGTCGACG 1 cut(s) 791
SinI GGWCC 7 cut(s) 58, 118, 521, 622, 658, 776, 1808
SlaI CTCGAG 1 cut(s) 461
SmiMI CAYNNNNRTG 3 cut(s) 1373, 1544, 1793
SmlI CTYRAG 5 cut(s) 461, 1223, 1313, 1460, 1643
SmoI CTYRAG 5 cut(s) 461, 1223, 1313, 1460, 1643
Sse9I AATT 3 cut(s) 148, 294, 515
SseBI AGGCCT 1 cut(s) 1296
SspDI GGCGCC 1 cut(s) 614
SspMI CTAG 1 cut(s) 1035
SstI GAGCTC 1 cut(s) 474
StuI AGGCCT 1 cut(s) 1296
StyD4I CCNGG 8 cut(s) 441, 490, 654, 704, 723, 766, 894, 1278
TaaI ACNGT 3 cut(s) 9, 208, 1457
TaiI ACGT 2 cut(s) 45, 52
TaqI TCGA 9 cut(s) 36, 74, 396, 462, 468, 756, 792, 852, 1260
TaqII GACCGA 1 cut(s) 956
TasI AATT 3 cut(s) 148, 294, 515
TatI WGTACW 4 cut(s) 9, 420, 729, 1040
TauI GCSGC 8 cut(s) 414, 417, 459, 510, 668, 671, 674, 695
Tru1I TTAA 3 cut(s) 293, 1062, 1494
Tru9I TTAA 3 cut(s) 293, 1062, 1494
TscAI CASTG 5 cut(s) 337, 1153, 1540, 1591, 1798
TseFI GTSAC 3 cut(s) 45, 1115, 1427
TseI GCWGC 4 cut(s) 408, 1214, 1217, 1724
Tsp45I GTSAC 3 cut(s) 45, 1115, 1427
TspDTI ATGAA 5 cut(s) 92, 980, 1412, 1583, 1721
TspGWI ACGGA 5 cut(s) 123, 382, 1122, 1165, 1206
TspRI CASTG 5 cut(s) 337, 1153, 1540, 1591, 1798
VneI GTGCAC 1 cut(s) 739
VpaK11BI GGWCC 7 cut(s) 58, 118, 521, 622, 658, 776, 1808
VspI ATTAAT 1 cut(s) 1062
XapI RAATTY 2 cut(s) 148, 515
XceI RCATGY 2 cut(s) 439, 1453
XhoI CTCGAG 1 cut(s) 461
XmiI GTMKAC 2 cut(s) 137, 792
XmnI GAANNNNTTC 2 cut(s) 1171, 1296
XspI CTAG 1 cut(s) 1035
ZrmI AGTACT 2 cut(s) 731, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.