pycom420g00980

Adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0009985
Physical Location & Seq
Reverse (-)
1291419 .. 1293701
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1446 bp
ATGCCCGGCCTCATCTCATGCCGCACCGAATTCGGACCCTCCCAACCCTTCAAGGGGGCCCGCATCACCGGTTCCCTCCACATGACCATCCAAACCGCTGTCCTCATTGAAACCCTCACCGCCCTCGGCGCCGAGGTCCGCTGGTGCTCCTGCAACATTTTCTCCACCCAGGACCACGCCGCCGCCGCCATCGCCCGCGATAGCGCCGCCGTCTTCGCCTGGAAGGGCGAGACCCTCCAGGAGTACTGGTGGTGCACCGAGCGCGCCCTCGACTGGGGCCCCGGTGGCGGACCCGATCTGATCGTCGACGACGGCGGTGACGCCACCCTCTTGATCCACGAGGGAGTCAAGGCTGAGGAGGAGTTCGAGAAGTCCGGTACCCTCCCTGACCCGTCTTCCACCGATAACCAGGAGTTCCAGCTGGTTCTGACCATCATCAGAGATGGCTTGAATACCGACCCCAAGAGGTACCACAAGATGAAGGACAGGTTGGTCGGAGTTTCGGAGGAGACCACCACCGGCGTCAAGAGGCTGTATCAGATGCAGGCTAGCAGTACTCTGTTGTTCCCCGCCATTAATGTCAATGACTCTGTTACAAAAAGCAAGTTTGACAACTTGTACGGATGCCGTCACTCTCTCCCTGATGGTTTGATGAGGGCCATTGATGTCATGATTGCCGGAAAGGTTTCCGTTGTTTGCGGATACGGAGATGTTGGAAAGGGTTGCGCTGCTGCCCTCAAGCAAGCAGGATCTCGCGTGATTGTGACTGAGATCGATCCAATCTGTGCCCTCCAGGCTCTTATGGAAGGCCTTCAGGTTCTTCCTCTTGAGGATGTTGTCTCCGAGGGTGATGAGGATGTTGTCTCCGTGGGTGATGAAGATGTTGTCTCCGTGGGTGATGAGGATGTTGTCTCCGAGGGTGATGAGGATGTTGTCTCCGAGGGTGATGAGGATGTTGTCTCCGAGGCTGATATCTTTGTTACCACCACCGGTAACAAGGACATCATCATGGTTGACCACATGAGGAAGATGAAGAACAATGCCATTGTTTGCAACATTGGTCACTTTGACAATGAGATTGACATGCACGGTCTTGAGACATACGCCGGAGTGAAGCGCATCACCATTAAGCCTCAAACTGACAGGTGGGTCTTCCCAGAGACCAACACTGGCATCATTGTGTTGGCTGAGGGCCTTCATCTTGGAAAGCTTGGAGCTAAGCTCACCAAACTCAGCAAGGAACAGGCTGACTACATCAGTGTGCCGGTTGAGGGTCCATACAAGCCAGCGCACTACAGGGTAATAATTTTGACACTTCTGGTTGGACGAAGATCCTCATCGGATCCTCTCCGGATCCTACTCCCTTCTGGTTTCTCCTTTTGCACCACTCTAGTGGTTCCCAAGGCGGACTGGACCGGTGTGCATGAGAAGAAAATGGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

482

Amino Acids

52.23

Weight (kDa)

4.81

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 1 - 433 8.3e-104 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 205 - 299 1.4e-41 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
AdoHcyase_NAD PF00670 317 - 399 1.9e-30 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 491, 1148
Acc65I GGTACC 2 cut(s) 377, 468
AccB1I GGYRCC 3 cut(s) 128, 377, 468
AccI GTMKAC 1 cut(s) 306
AccII CGCG 3 cut(s) 198, 264, 756
AccIII TCCGGA 1 cut(s) 1350
AclWI GGATC 8 cut(s) 328, 757, 770, 1326, 1337, 1348, 1350, 1361
AcsI RAATTY 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 797
AcyI GRCGYC 3 cut(s) 129, 321, 522
AfaI GTAC 5 cut(s) 245, 379, 470, 556, 620
AfiI CCNNNNNNNGG 6 cut(s) 53, 54, 273, 274, 287, 1271
AgeI ACCGGT 3 cut(s) 68, 989, 1415
AgsI TTSAA 3 cut(s) 52, 110, 451
AjnI CCWGG 5 cut(s) 168, 218, 237, 408, 792
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AleI CACNNNNGTG 1 cut(s) 1391
AluBI AGCT 4 cut(s) 421, 1210, 1217, 1222
AluI AGCT 4 cut(s) 421, 1210, 1217, 1222
Alw21I GWGCWC 2 cut(s) 149, 257
Alw44I GTGCAC 1 cut(s) 253
AlwI GGATC 8 cut(s) 328, 757, 770, 1326, 1337, 1348, 1350, 1361
Aor13HI TCCGGA 1 cut(s) 1350
AoxI GGCC 6 cut(s) 7, 57, 277, 657, 808, 1192
ApaI GGGCCC 2 cut(s) 61, 281
ApaLI GTGCAC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 728, 731
ApoI RAATTY 1 cut(s) 29
AseI ATTAAT 1 cut(s) 576
AsiGI ACCGGT 3 cut(s) 68, 989, 1415
Asp700I GAANNNNTTC 2 cut(s) 685, 810
Asp718I GGTACC 2 cut(s) 377, 468
AspLEI GCGC 7 cut(s) 131, 206, 264, 266, 728, 1119, 1291
AsuC2I CCSGG 2 cut(s) 6, 282
AsuNHI GCTAGC 1 cut(s) 548
AvaII GGWCC 6 cut(s) 35, 136, 172, 290, 1274, 1413
BaeGI GKGCMC 4 cut(s) 61, 257, 281, 790
BamHI GGATCC 2 cut(s) 1342, 1353
BanI GGYRCC 3 cut(s) 128, 377, 468
BanII GRGCYC 2 cut(s) 61, 281
BauI CACGAG 1 cut(s) 338
BbsI GAAGAC 3 cut(s) 205, 387, 1144
Bbv12I GWGCWC 2 cut(s) 149, 257
BbvCI CCTCAGC 2 cut(s) 354, 1188
BbvI GCAGC 2 cut(s) 715, 718
BccI CCATC 5 cut(s) 95, 197, 437, 440, 638
BceAI ACGGC 3 cut(s) 194, 328, 612
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 5 cut(s) 170, 220, 239, 410, 794
BciVI GTATCC 1 cut(s) 695
BcnI CCSGG 2 cut(s) 6, 282
BfaI CTAG 2 cut(s) 549, 1391
BfmI CTRYAG 1 cut(s) 1294
BfoI RGCGCY 2 cut(s) 132, 207
BfuI GTATCC 1 cut(s) 695
BglI GCCNNNNNGGC 2 cut(s) 285, 794
BisI GCNGC 7 cut(s) 22, 180, 183, 186, 207, 729, 732
BlpI GCTNAGC 1 cut(s) 1218
BlsI GCNGC 7 cut(s) 23, 181, 184, 187, 208, 730, 733
BmcAI AGTACT 2 cut(s) 245, 556
Bme1390I CCNGG 7 cut(s) 6, 170, 220, 239, 282, 410, 794
Bme18I GGWCC 6 cut(s) 35, 136, 172, 290, 1274, 1413
BmrFI CCNGG 7 cut(s) 6, 170, 220, 239, 282, 410, 794
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 5 cut(s) 72, 531, 614, 1128, 1182
BmtI GCTAGC 1 cut(s) 552
BmuI ACTGGG 1 cut(s) 283
BpiI GAAGAC 3 cut(s) 205, 387, 1144
BplI GAGNNNNNCTC 2 cut(s) 1206, 1238
BpmI CTGGAG 2 cut(s) 221, 776
Bpu10I CCTNAGC 2 cut(s) 354, 1188
Bpu1102I GCTNAGC 1 cut(s) 1218
BpuEI CTTGAG 3 cut(s) 722, 848, 1115
BpuMI CCSGG 2 cut(s) 6, 282
Bsa29I ATCGAT 1 cut(s) 774
BsaBI GATNNNNATC 1 cut(s) 1336
BsaHI GRCGYC 3 cut(s) 129, 321, 522
BsaI GGTCTC 3 cut(s) 224, 503, 1154
BsaWI WCCGGW 5 cut(s) 68, 374, 989, 1350, 1415
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 233, 263
Bsc4I CCNNNNNNNGG 6 cut(s) 53, 54, 273, 274, 287, 1271
Bse118I RCCGGY 5 cut(s) 68, 518, 989, 1264, 1415
Bse1I ACTGG 4 cut(s) 251, 278, 1174, 1415
Bse8I GATNNNNATC 1 cut(s) 1336
BseAI TCCGGA 1 cut(s) 1350
BseBI CCWGG 5 cut(s) 170, 220, 239, 410, 794
BseCI ATCGAT 1 cut(s) 774
BseGI GGATG 7 cut(s) 87, 629, 838, 862, 910, 934, 958
BseJI GATNNNNATC 1 cut(s) 1336
BseLI CCNNNNNNNGG 6 cut(s) 53, 54, 273, 274, 287, 1271
BseMII CTCAG 4 cut(s) 345, 759, 1179, 1246
BseNI ACTGG 4 cut(s) 251, 278, 1174, 1415
BsePI GCGCGC 1 cut(s) 262
BseRI GAGGAG 3 cut(s) 371, 374, 521
BseSI GKGCMC 4 cut(s) 61, 257, 281, 790
BseXI GCAGC 2 cut(s) 715, 718
Bsh1236I CGCG 3 cut(s) 198, 264, 756
BshFI GGCC 6 cut(s) 9, 59, 279, 659, 810, 1194
BshNI GGYRCC 3 cut(s) 128, 377, 468
BshTI ACCGGT 3 cut(s) 68, 989, 1415
BshVI ATCGAT 1 cut(s) 774
BsiHKAI GWGCWC 2 cut(s) 149, 257
BslI CCNNNNNNNGG 6 cut(s) 53, 54, 273, 274, 287, 1271
BsnI GGCC 6 cut(s) 9, 59, 279, 659, 810, 1194
Bso31I GGTCTC 3 cut(s) 224, 503, 1154
Bsp120I GGGCCC 2 cut(s) 57, 277
Bsp1286I GDGCHC 5 cut(s) 61, 149, 257, 281, 790
Bsp13I TCCGGA 1 cut(s) 1350
Bsp143I GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
Bsp1720I GCTNAGC 1 cut(s) 1218
BspANI GGCC 6 cut(s) 9, 59, 279, 659, 810, 1194
BspCNI CTCAG 4 cut(s) 346, 760, 1180, 1245
BspDI ATCGAT 1 cut(s) 774
BspEI TCCGGA 1 cut(s) 1350
BspFNI CGCG 3 cut(s) 198, 264, 756
BspHI TCATGA 1 cut(s) 669
BspOI GCTAGC 1 cut(s) 552
BspPI GGATC 8 cut(s) 328, 757, 770, 1326, 1337, 1348, 1350, 1361
BspT107I GGYRCC 3 cut(s) 128, 377, 468
BspTNI GGTCTC 3 cut(s) 224, 503, 1154
BsrFI RCCGGY 5 cut(s) 68, 518, 989, 1264, 1415
BsrI ACTGG 4 cut(s) 251, 278, 1174, 1415
BssAI RCCGGY 5 cut(s) 68, 518, 989, 1264, 1415
BssHII GCGCGC 1 cut(s) 262
BssMI GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
BssNI GRCGYC 3 cut(s) 129, 321, 522
BssSI CACGAG 1 cut(s) 338
BssT1I CCWWGG 1 cut(s) 1401
Bst2BI CACGAG 1 cut(s) 338
Bst2UI CCWGG 5 cut(s) 170, 220, 239, 410, 794
Bst4CI ACNGT 1 cut(s) 1091
BstACI GRCGYC 3 cut(s) 129, 321, 522
BstC8I GCNNGC 7 cut(s) 61, 196, 264, 546, 550, 744, 1287
BstDEI CTNAG 5 cut(s) 354, 768, 1188, 1218, 1232
BstDSI CCRYGG 2 cut(s) 867, 891
BstF5I GGATG 7 cut(s) 87, 629, 838, 862, 910, 934, 958
BstFNI CGCG 3 cut(s) 198, 264, 756
BstH2I RGCGCY 2 cut(s) 132, 207
BstHHI GCGC 7 cut(s) 131, 206, 264, 266, 728, 1119, 1291
BstKTI GATC 9 cut(s) 298, 303, 336, 752, 774, 778, 1334, 1345, 1356
BstMBI GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
BstMWI GCNNNNNNNGC 7 cut(s) 128, 185, 191, 215, 261, 285, 794
BstNI CCWGG 5 cut(s) 170, 220, 239, 410, 794
BstNSI RCATGY 1 cut(s) 1087
BstSCI CCNGG 7 cut(s) 4, 168, 218, 237, 280, 408, 792
BstSFI CTRYAG 1 cut(s) 1294
BstSLI GKGCMC 4 cut(s) 61, 257, 281, 790
BstUI CGCG 3 cut(s) 198, 264, 756
BstV1I GCAGC 2 cut(s) 715, 718
BstV2I GAAGAC 3 cut(s) 205, 387, 1144
BstX2I RGATCY 4 cut(s) 749, 1331, 1342, 1353
BstXI CCANNNNNNTGG 1 cut(s) 1393
BstYI RGATCY 4 cut(s) 749, 1331, 1342, 1353
Bsu15I ATCGAT 1 cut(s) 774
BsuI GTATCC 1 cut(s) 695
BsuRI GGCC 6 cut(s) 9, 59, 279, 659, 810, 1194
BsuTUI ATCGAT 1 cut(s) 774
BtgI CCRYGG 2 cut(s) 867, 891
BtgZI GCGATG 1 cut(s) 175
BtsCI GGATG 7 cut(s) 87, 629, 838, 862, 910, 934, 958
BtsIMutI CAGTG 2 cut(s) 1167, 1264
Cac8I GCNNGC 7 cut(s) 61, 196, 264, 546, 550, 744, 1287
CciI TCATGA 1 cut(s) 669
CfoI GCGC 7 cut(s) 131, 206, 264, 266, 728, 1119, 1291
Cfr10I RCCGGY 5 cut(s) 68, 518, 989, 1264, 1415
ClaI ATCGAT 1 cut(s) 774
CseI GACGC 2 cut(s) 329, 511
Csp6I GTAC 5 cut(s) 244, 378, 469, 555, 619
CspAI ACCGGT 3 cut(s) 68, 989, 1415
CviAII CATG 7 cut(s) 18, 82, 670, 1009, 1021, 1084, 1424
CviQI GTAC 5 cut(s) 244, 378, 469, 555, 619
DdeI CTNAG 5 cut(s) 354, 768, 1188, 1218, 1232
DinI GGCGCC 1 cut(s) 130
DpnI GATC 9 cut(s) 297, 302, 335, 751, 773, 777, 1333, 1344, 1355
DpnII GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
DrdI GACNNNNNNGTC 2 cut(s) 491, 1148
DseDI GACNNNNNNGTC 2 cut(s) 491, 1148
EciI GGCGGA 2 cut(s) 303, 1421
Eco130I CCWWGG 1 cut(s) 1401
Eco147I AGGCCT 1 cut(s) 810
Eco24I GRGCYC 2 cut(s) 61, 281
Eco31I GGTCTC 3 cut(s) 224, 503, 1154
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 6 cut(s) 35, 136, 172, 290, 1274, 1413
Eco57I CTGAAG 1 cut(s) 797
EcoO109I RGGNCCY 4 cut(s) 57, 277, 278, 1192
EcoRI GAATTC 1 cut(s) 29
EcoRII CCWGG 5 cut(s) 168, 218, 237, 408, 792
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 1 cut(s) 1401
EcoT38I GRGCYC 2 cut(s) 61, 281
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 1401
FaeI CATG 7 cut(s) 21, 85, 673, 1012, 1024, 1087, 1427
FatI CATG 7 cut(s) 17, 81, 669, 1008, 1020, 1083, 1423
FauI CCCGC 3 cut(s) 68, 203, 577
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 7 cut(s) 22, 180, 183, 186, 207, 729, 732
FokI GGATG 7 cut(s) 74, 636, 845, 869, 917, 941, 965
FriOI GRGCYC 2 cut(s) 61, 281
Fsp4HI GCNGC 7 cut(s) 22, 180, 183, 186, 207, 729, 732
FspBI CTAG 2 cut(s) 549, 1391
GlaI GCGC 7 cut(s) 130, 205, 263, 265, 727, 1118, 1290
GluI GCNGC 7 cut(s) 22, 180, 183, 186, 207, 729, 732
GsuI CTGGAG 2 cut(s) 221, 776
HaeII RGCGCY 2 cut(s) 132, 207
HaeIII GGCC 6 cut(s) 9, 59, 279, 659, 810, 1194
HgaI GACGC 2 cut(s) 329, 511
HhaI GCGC 7 cut(s) 131, 206, 264, 266, 728, 1119, 1291
Hin1I GRCGYC 3 cut(s) 129, 321, 522
Hin1II CATG 7 cut(s) 21, 85, 673, 1012, 1024, 1087, 1427
Hin6I GCGC 7 cut(s) 129, 204, 262, 264, 726, 1117, 1289
HinP1I GCGC 7 cut(s) 129, 204, 262, 264, 726, 1117, 1289
HincII GTYRAC 2 cut(s) 307, 1015
HindII GTYRAC 2 cut(s) 307, 1015
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 2 cut(s) 345, 587
Hpy166II GTNNAC 3 cut(s) 255, 307, 1015
Hpy188III TCNNGA 7 cut(s) 331, 367, 526, 670, 827, 1094, 1351
Hpy8I GTNNAC 3 cut(s) 255, 307, 1015
Hpy99I CGWCG 3 cut(s) 308, 311, 314
HpyAV CCTTC 7 cut(s) 58, 217, 475, 800, 821, 1205, 1374
HpyCH4III ACNGT 1 cut(s) 1091
HpyCH4V TGCA 7 cut(s) 153, 255, 544, 1053, 1087, 1383, 1423
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 185, 191, 215, 261, 285, 794
HpyF3I CTNAG 5 cut(s) 354, 768, 1188, 1218, 1232
Hsp92I GRCGYC 3 cut(s) 129, 321, 522
Hsp92II CATG 7 cut(s) 21, 85, 673, 1012, 1024, 1087, 1427
HspAI GCGC 7 cut(s) 129, 204, 262, 264, 726, 1117, 1289
KasI GGCGCC 1 cut(s) 128
Kpn2I TCCGGA 1 cut(s) 1350
KpnI GGTACC 2 cut(s) 381, 472
Kzo9I GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
LmnI GCTCC 2 cut(s) 152, 1214
Lsp1109I GCAGC 2 cut(s) 715, 718
LweI GCATC 5 cut(s) 72, 531, 614, 1128, 1182
MaeI CTAG 2 cut(s) 549, 1391
MaeIII GTNAC 7 cut(s) 317, 592, 629, 763, 979, 992, 1061
MalI GATC 9 cut(s) 297, 302, 335, 751, 773, 777, 1333, 1344, 1355
MboI GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
MboII GAAGA 9 cut(s) 205, 387, 812, 890, 1039, 1045, 1144, 1341, 1441
MflI RGATCY 4 cut(s) 749, 1331, 1342, 1353
MhlI GDGCHC 5 cut(s) 61, 149, 257, 281, 790
MluCI AATT 2 cut(s) 29, 1305
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 2 cut(s) 354, 581
MmeI TCCRAC 3 cut(s) 475, 694, 1303
MroI TCCGGA 1 cut(s) 1350
MroXI GAANNNNTTC 2 cut(s) 685, 810
MseI TTAA 2 cut(s) 576, 1128
MslI CAYNNNNRTG 4 cut(s) 1007, 1178, 1259, 1391
MspA1I CMGCKG 3 cut(s) 98, 141, 421
MspR9I CCNGG 7 cut(s) 6, 170, 220, 239, 282, 410, 794
MvaI CCWGG 5 cut(s) 170, 220, 239, 410, 794
MvnI CGCG 3 cut(s) 198, 264, 756
MwoI GCNNNNNNNGC 7 cut(s) 128, 185, 191, 215, 261, 285, 794
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 2 cut(s) 6, 282
NdeII GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
NheI GCTAGC 1 cut(s) 548
NlaIII CATG 7 cut(s) 21, 85, 673, 1012, 1024, 1087, 1427
NmeAIII GCCGAG 2 cut(s) 105, 157
NmuCI GTSAC 4 cut(s) 317, 629, 763, 1061
NspI RCATGY 1 cut(s) 1087
OliI CACNNNNGTG 1 cut(s) 1391
PagI TCATGA 1 cut(s) 669
PauI GCGCGC 1 cut(s) 262
PceI AGGCCT 1 cut(s) 810
PdmI GAANNNNTTC 2 cut(s) 685, 810
PfoI TCCNGGA 1 cut(s) 237
PinAI ACCGGT 3 cut(s) 68, 989, 1415
PkrI GCNGC 7 cut(s) 23, 181, 184, 187, 208, 730, 733
PleI GAGTC 2 cut(s) 353, 581
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 2 cut(s) 353, 581
PshBI ATTAAT 1 cut(s) 576
Psp6I CCWGG 5 cut(s) 168, 218, 237, 408, 792
PspGI CCWGG 5 cut(s) 168, 218, 237, 408, 792
PspOMI GGGCCC 2 cut(s) 57, 277
PsuI RGATCY 4 cut(s) 749, 1331, 1342, 1353
PteI GCGCGC 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 421
RsaI GTAC 5 cut(s) 245, 379, 470, 556, 620
RsaNI GTAC 5 cut(s) 244, 378, 469, 555, 619
RseI CAYNNNNRTG 4 cut(s) 1007, 1178, 1259, 1391
SalI GTCGAC 1 cut(s) 305
SaqAI TTAA 2 cut(s) 576, 1128
SatI GCNGC 7 cut(s) 22, 180, 183, 186, 207, 729, 732
Sau3AI GATC 9 cut(s) 295, 300, 333, 749, 771, 775, 1331, 1342, 1353
ScaI AGTACT 2 cut(s) 245, 556
SchI GAGTC 2 cut(s) 354, 581
ScrFI CCNGG 7 cut(s) 6, 170, 220, 239, 282, 410, 794
SduI GDGCHC 5 cut(s) 61, 149, 257, 281, 790
SfaNI GCATC 5 cut(s) 72, 531, 614, 1128, 1182
SfcI CTRYAG 1 cut(s) 1294
SfoI GGCGCC 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 518
SgrDI CGTCGACG 1 cut(s) 305
SinI GGWCC 6 cut(s) 35, 136, 172, 290, 1274, 1413
SmiMI CAYNNNNRTG 4 cut(s) 1007, 1178, 1259, 1391
SmlI CTYRAG 3 cut(s) 737, 827, 1094
SmoI CTYRAG 3 cut(s) 737, 827, 1094
Sse9I AATT 2 cut(s) 29, 1305
SseBI AGGCCT 1 cut(s) 810
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 2 cut(s) 549, 1391
StuI AGGCCT 1 cut(s) 810
StyD4I CCNGG 7 cut(s) 4, 168, 218, 237, 280, 408, 792
StyI CCWWGG 1 cut(s) 1401
TaaI ACNGT 1 cut(s) 1091
TaqI TCGA 4 cut(s) 270, 306, 366, 774
TasI AATT 2 cut(s) 29, 1305
TatI WGTACW 2 cut(s) 243, 554
TauI GCSGC 5 cut(s) 24, 182, 185, 188, 209
Tru1I TTAA 2 cut(s) 576, 1128
Tru9I TTAA 2 cut(s) 576, 1128
TscAI CASTG 2 cut(s) 1174, 1264
TseFI GTSAC 4 cut(s) 317, 629, 763, 1061
TseI GCWGC 2 cut(s) 728, 731
Tsp45I GTSAC 4 cut(s) 317, 629, 763, 1061
TspDTI ATGAA 4 cut(s) 494, 891, 1046, 1187
TspGWI ACGGA 5 cut(s) 636, 679, 720, 856, 880
TspRI CASTG 2 cut(s) 1174, 1264
VneI GTGCAC 1 cut(s) 253
VpaK11BI GGWCC 6 cut(s) 35, 136, 172, 290, 1274, 1413
VspI ATTAAT 1 cut(s) 576
XapI RAATTY 1 cut(s) 29
XceI RCATGY 1 cut(s) 1087
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 2 cut(s) 685, 810
XspI CTAG 2 cut(s) 549, 1391
ZrmI AGTACT 2 cut(s) 245, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.