RchiOBHm_Chr7g0210311

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0018716
Physical Location & Seq
Forward (+)
27946981 .. 27948215
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1122 bp
ATGAGAATACACAGCAAATTCCTCTCAAATTCAGTTTCTCTACAACATGCCAACCACGAACCAACAATCCATCAACGAAGAAATTACAAGCTGTGGTTTCGAATAGCAACCTATATATTCTTTGCCCTCTCCGGTCAATCAACAGCAACACTTCTGGGAAGATTCTACTTCGAGAAAGGTGGAAAGAGCAAATGGGTGGGGGCTCTGGTGCAACTCGCAGGATTTCCAATCCTCCTTCCTTTCTATTTCTTCGTATCCTCCAAAATTAGTCGCACTCCAAGTACTACTGTTGCTGCTGCTTGTCCTATCCAATATTCAAAACCCACGTCTTTGTTAACAACCCTAGCATTGGTTTATGTTACCCTAGGGCTACTCGTAGCAGGAATCTGCTTCTTGACTTCATTTGGGCTTCAATACCTTCCTGTCTCTACTTTCTCACTCATTTGTGCTTCTCAATTGGGATTCACAGCTCTCTTTTCCTTTTTCCTAAACTCCCAAAAGTTCACTCCATTTGTGATTAATTCCATTGTGCTACTCACCATTTCCTCCATTCTCCTTGTGTTCAATACCGACTCATCATCAGTATTAGTCTCTAAAGCTAAGTACAAAGTCGGATTCATATGCACCATTGGCGCTGCTGCTGGGAACGGATTAGCACTTGCCTTAACCCAGTTCGCCTTCACGAAAATAATCAAGAAGCAAACATTTAGAGCAGTGGTGGATTTGATAATCTATGAGTCACTAATAGCAACATGTGCTACTTTGGTCGGACTTTTTGCTAGTGGAGAGTGGAAGGGCTTAAATCTAGAGATGGATGAGTTTCAAACAGGAAAGGGATCTTATGTGTTGACTCTAGCTTGGACAATGATTTCTTGGCAAGTTTTTGCTATTGGTGCAGTGGGATTGATATTTGAGGTTTCTTCCCTCTTCTGCAATGTTATAATTGCTTTGGGTTTGCCCGTGGTTCAAGTTTTGGCTGTGATTGTTTTCCATGACAGGATGCATGGTTTGAAGATTGTGGCAATAGTTTTGGCCCTATGGGGTTTCATTTCATATGTCTATCAGCAGTATCTTGATGACCATTGCAAATCATTCGAAAGTACTGAAAATGAAAATGCATGA

Protein Analysis

373

Amino Acids

41.08

Weight (kDa)

9.24

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 35 - 360 7.1e-116 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0018716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18190
rosa_chinensis RchiOBHm_Chr7g0210311
rosa_laevigata RLG00000003050
rosa_multiflora Rmu_sc0004503.1_g000004
rosa_roxburghii Rroxscaffold_3G00248440
rosa_samantha Rh7AG253500 Rh7CG270400 Rh7DG261100
rosa_wichuraiana Rw7G021610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 941
AclWI GGATC 1 cut(s) 844
AcsI RAATTY 2 cut(s) 17, 28
AfaI GTAC 3 cut(s) 283, 605, 1102
AfiI CCNNNNNNNGG 1 cut(s) 349
AflIII ACRYGT 1 cut(s) 752
AgsI TTSAA 6 cut(s) 318, 413, 565, 824, 968, 1012
AjiI CACGTC 1 cut(s) 327
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
AluBI AGCT 4 cut(s) 91, 470, 599, 857
AluI AGCT 4 cut(s) 91, 470, 599, 857
Alw26I GTCTC 2 cut(s) 430, 595
AlwI GGATC 1 cut(s) 844
AoxI GGCC 1 cut(s) 1032
ApeKI GCWGC 4 cut(s) 293, 296, 635, 638
ApoI RAATTY 2 cut(s) 17, 28
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AseI ATTAAT 1 cut(s) 519
AspA2I CCTAGG 1 cut(s) 364
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 1 cut(s) 1033
AsuHPI GGTGA 1 cut(s) 529
AsuII TTCGAA 2 cut(s) 100, 1095
AvrII CCTAGG 1 cut(s) 364
BanII GRGCYC 1 cut(s) 205
BbvI GCAGC 4 cut(s) 280, 283, 622, 625
BccI CCATC 2 cut(s) 78, 805
BcgI CGANNNNNNTGC 2 cut(s) 1075, 1109
BciVI GTATCC 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 430, 595
BfaI CTAG 5 cut(s) 344, 365, 780, 806, 854
BfoI RGCGCY 1 cut(s) 636
BfuI GTATCC 1 cut(s) 265
BisI GCNGC 4 cut(s) 294, 297, 636, 639
BlnI CCTAGG 1 cut(s) 364
BlsI GCNGC 4 cut(s) 295, 298, 637, 640
BmcAI AGTACT 2 cut(s) 283, 1102
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 1033
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 1 cut(s) 990
BmuI ACTGGG 1 cut(s) 664
Bpu14I TTCGAA 2 cut(s) 100, 1095
BsaJI CCNNGG 2 cut(s) 364, 960
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 670
Bse3DI GCAATG 2 cut(s) 940, 1081
BseDI CCNNGG 2 cut(s) 364, 960
BseGI GGATG 2 cut(s) 820, 1005
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMI GCAATG 2 cut(s) 940, 1081
BseNI ACTGG 1 cut(s) 670
BseXI GCAGC 4 cut(s) 280, 283, 622, 625
BseYI CCCAGC 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 915
BshFI GGCC 1 cut(s) 1034
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 349
BsmAI GTCTC 2 cut(s) 430, 595
BsnI GGCC 1 cut(s) 1034
Bsp119I TTCGAA 2 cut(s) 100, 1095
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 1 cut(s) 836
BspANI GGCC 1 cut(s) 1034
BspPI GGATC 1 cut(s) 844
BspT104I TTCGAA 2 cut(s) 100, 1095
BsrDI GCAATG 2 cut(s) 940, 1081
BsrI ACTGG 1 cut(s) 670
BssECI CCNNGG 2 cut(s) 364, 960
BssMI GATC 1 cut(s) 836
BssT1I CCWWGG 1 cut(s) 364
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 932
BstAPI GCANNNNNTGC 1 cut(s) 755
BstBI TTCGAA 2 cut(s) 100, 1095
BstDEI CTNAG 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 2 cut(s) 820, 1005
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 1 cut(s) 839
BstMAI GTCTC 2 cut(s) 430, 595
BstMBI GATC 1 cut(s) 836
BstMWI GCNNNNNNNGC 3 cut(s) 630, 755, 893
BstNSI RCATGY 2 cut(s) 50, 756
BstV1I GCAGC 4 cut(s) 280, 283, 622, 625
BstX2I RGATCY 1 cut(s) 836
BstYI RGATCY 1 cut(s) 836
BsuI GTATCC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 1034
BtgI CCRYGG 1 cut(s) 960
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 2 cut(s) 820, 1005
BtsI GCAGTG 2 cut(s) 720, 903
BtsIMutI CAGTG 2 cut(s) 720, 903
CfoI GCGC 1 cut(s) 635
Cfr13I GGNCC 1 cut(s) 1033
Csp6I GTAC 3 cut(s) 282, 604, 1101
CviAII CATG 5 cut(s) 47, 753, 992, 1004, 1119
CviQI GTAC 3 cut(s) 282, 604, 1101
DdeI CTNAG 1 cut(s) 600
DpnI GATC 1 cut(s) 838
DpnII GATC 1 cut(s) 836
Eam1104I CTCTTC 1 cut(s) 932
EarI CTCTTC 1 cut(s) 932
Eco130I CCWWGG 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 364
EcoT22I ATGCAT 2 cut(s) 1005, 1120
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 5 cut(s) 50, 756, 995, 1007, 1122
FatI CATG 5 cut(s) 46, 752, 991, 1003, 1118
FauNDI CATATG 2 cut(s) 620, 1054
Fnu4HI GCNGC 4 cut(s) 294, 297, 636, 639
FokI GGATG 2 cut(s) 827, 1012
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 4 cut(s) 294, 297, 636, 639
FspBI CTAG 5 cut(s) 344, 365, 780, 806, 854
GlaI GCGC 1 cut(s) 634
GluI GCNGC 4 cut(s) 294, 297, 636, 639
GsaI CCCAGC 1 cut(s) 645
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 1 cut(s) 1034
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 635
Hin1II CATG 5 cut(s) 50, 756, 995, 1007, 1122
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 2 cut(s) 336, 849
HindII GTYRAC 2 cut(s) 336, 849
HinfI GANTC 7 cut(s) 162, 384, 462, 572, 615, 737, 850
HpaI GTTAAC 1 cut(s) 336
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 3 cut(s) 336, 504, 849
Hpy188I TCNGA 2 cut(s) 614, 770
Hpy188III TCNNGA 6 cut(s) 172, 394, 682, 694, 806, 1073
Hpy8I GTNNAC 3 cut(s) 336, 504, 849
HpyAV CCTTC 4 cut(s) 245, 428, 688, 787
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 7 cut(s) 211, 624, 896, 933, 1003, 1086, 1118
HpyF10VI GCNNNNNNNGC 3 cut(s) 630, 755, 893
HpyF3I CTNAG 1 cut(s) 600
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 5 cut(s) 50, 756, 995, 1007, 1122
HspAI GCGC 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 336
Kzo9I GATC 1 cut(s) 836
Lsp1109I GCAGC 4 cut(s) 280, 283, 622, 625
LweI GCATC 1 cut(s) 990
MaeI CTAG 5 cut(s) 344, 365, 780, 806, 854
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 2 cut(s) 358, 738
MalI GATC 1 cut(s) 838
MboI GATC 1 cut(s) 836
MboII GAAGA 6 cut(s) 90, 171, 241, 912, 919, 1024
MfeI CAATTG 1 cut(s) 455
MflI RGATCY 1 cut(s) 836
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 7 cut(s) 17, 28, 82, 264, 455, 520, 942
MlyI GAGTC 3 cut(s) 566, 746, 844
MmeI TCCRAC 2 cut(s) 592, 748
MnlI CCTC 7 cut(s) 32, 137, 242, 268, 556, 907, 935
Mph1103I ATGCAT 2 cut(s) 1005, 1120
MseI TTAA 4 cut(s) 335, 519, 665, 800
MspI CCGG 1 cut(s) 132
MunI CAATTG 1 cut(s) 455
MwoI GCNNNNNNNGC 3 cut(s) 630, 755, 893
NdeI CATATG 2 cut(s) 620, 1054
NdeII GATC 1 cut(s) 836
NlaIII CATG 5 cut(s) 50, 756, 995, 1007, 1122
NmuCI GTSAC 1 cut(s) 738
NsiI ATGCAT 2 cut(s) 1005, 1120
NspI RCATGY 2 cut(s) 50, 756
NspV TTCGAA 2 cut(s) 100, 1095
PciI ACATGT 1 cut(s) 752
PfeI GAWTC 4 cut(s) 162, 384, 462, 615
PkrI GCNGC 4 cut(s) 295, 298, 637, 640
PleI GAGTC 3 cut(s) 566, 745, 844
PpsI GAGTC 3 cut(s) 566, 745, 844
PscI ACATGT 1 cut(s) 752
PshBI ATTAAT 1 cut(s) 519
PsiI TTATAA 1 cut(s) 941
PspFI CCCAGC 1 cut(s) 641
PspPI GGNCC 1 cut(s) 1033
PsuI RGATCY 1 cut(s) 836
RsaI GTAC 3 cut(s) 283, 605, 1102
RsaNI GTAC 3 cut(s) 282, 604, 1101
SaqAI TTAA 4 cut(s) 335, 519, 665, 800
SatI GCNGC 4 cut(s) 294, 297, 636, 639
Sau3AI GATC 1 cut(s) 836
Sau96I GGNCC 1 cut(s) 1033
ScaI AGTACT 2 cut(s) 283, 1102
SchI GAGTC 3 cut(s) 566, 746, 844
SduI GDGCHC 1 cut(s) 205
SetI ASST 9 cut(s) 93, 113, 181, 329, 420, 472, 601, 859, 918
SfaNI GCATC 1 cut(s) 990
SfuI TTCGAA 2 cut(s) 100, 1095
Sse9I AATT 7 cut(s) 17, 28, 82, 264, 455, 520, 942
SspI AATATT 1 cut(s) 314
SspMI CTAG 5 cut(s) 344, 365, 780, 806, 854
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 329
TaqI TCGA 3 cut(s) 100, 171, 1095
TasI AATT 7 cut(s) 17, 28, 82, 264, 455, 520, 942
TatI WGTACW 3 cut(s) 281, 603, 1100
TfiI GAWTC 4 cut(s) 162, 384, 462, 615
Tru1I TTAA 4 cut(s) 335, 519, 665, 800
Tru9I TTAA 4 cut(s) 335, 519, 665, 800
TscAI CASTG 2 cut(s) 720, 903
TseFI GTSAC 1 cut(s) 738
TseI GCWGC 4 cut(s) 293, 296, 635, 638
Tsp45I GTSAC 1 cut(s) 738
TspDTI ATGAA 4 cut(s) 390, 607, 1036, 1041
TspGWI ACGGA 1 cut(s) 663
TspRI CASTG 2 cut(s) 720, 903
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 2 cut(s) 17, 28
XbaI TCTAGA 1 cut(s) 805
XceI RCATGY 2 cut(s) 50, 756
XmaJI CCTAGG 1 cut(s) 364
XspI CTAG 5 cut(s) 344, 365, 780, 806, 854
ZrmI AGTACT 2 cut(s) 283, 1102
Zsp2I ATGCAT 2 cut(s) 1005, 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.