Rroxscaffold_3G00248440

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0018716
Physical Location & Seq
Reverse (-)
41746911 .. 41749810
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248440.1

Sequence Viewer

Length: 1197 bp
ATGGCAGAGGCTCAGCAAGAAGTTCAACTTGGCATAAGTATTGAGTTGTCATTGGCTCACTTAATTTCCATTTGGGTTCCAGCTCAAGATAACAAAGAAGGAAACTCAACTGAGCTAACAAGTGCCAACCACGAACCAACAATCCATCATCGAAGAAATTACAAGCTGTGGTTTCGAATAGCAACCTATATATTCTTTGCCCTCTCCGGCCTATCAACAGGAACACTTCTGGGAAGATTCTACTTCGAGAAAGGTGGAAAGAGCAAATGGGTGGGGGCTCTGGTGCAACTCGCAGGATTTCCAATCCTCTTTCCTTTCTATTTCTTCGTATCCTCCAAAATTAGTCCCACTCCAAGTACTACTGCTGCTGCTGCTTGTCCTATCCAATATTCAAAACCCACGTCTTCGTTAACAACCCTAGCATTGGTTTATGTTACCCTAGGGCTACTCATAGCAGGAATCTGCTTCTTGACTTCATTTGGGCTTCAATACCTTCCTGTCTCTACGTTCTCACTCATTTGTGCTTCTCAATTGGGATTCACAGCTCTCTTTTCCTTTTTCCTAAACTCCCAAAAGTTCACTCCATTTGTGATTAATTCCATTGTGCTACTCACCATTTCCTCCATTCTCCTTGTGTTCAATACCGACTCATCATCAGTATTAGTCTCTAAAGCTAAGTACGAAGTCGGATTCATATGCACCATTGGCGCTGCTGCTGGGAACGGATTAGCACTTGCCTTAACCCAGTTCGCCTTCACGAAAATAATCAAGAAGCAAACATTTAGAGCAGTGGTGGATTTGATAATCTATGAGTCACTAGTAGCAACATGTGCTACTTTGGTCGGACTTTTTGCTAGTGGAGAGTGGAAGGGATTAAATCTAGAGATGGATGAGTTTCAAACAGGAAAGGGATCTTATGTGTTGACTCTAGCTTGGACAATGATTTCTTGGCAAGTTTTTATTATTGGTGCAGTGGGATTGATATTTGAGGTTTCTTCCCTCTTCAGCAATGTTATAATTGCTTTGGGTTTGCCCGTGGTTCAAGTTTTGGCTGTGATTGTTTTCCATGACAGGATGCATGGTTTGAAGATTGTGGCAATAGTTTTGGCGCTCTGGGGTTTCATTTCATATGTCTATCAGCACTATCTTGATGACCATTGCAAATCATTCGAAAGTACTGAAAATGAAAATGCATGA

Protein Analysis

398

Amino Acids

43.59

Weight (kDa)

7.07

Isoelectric Point (pI)

28.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 60 - 385 1.3e-115 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18190
rosa_chinensis RchiOBHm_Chr7g0210311
rosa_laevigata RLG00000003050
rosa_multiflora Rmu_sc0004503.1_g000004
rosa_roxburghii Rroxscaffold_3G00248440
rosa_samantha Rh7AG253500 Rh7CG270400 Rh7DG261100
rosa_wichuraiana Rw7G021610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AclWI GGATC 1 cut(s) 919
AcuI CTGAAG 1 cut(s) 988
AfaI GTAC 3 cut(s) 358, 680, 1177
AfiI CCNNNNNNNGG 1 cut(s) 424
AflIII ACRYGT 1 cut(s) 827
AgsI TTSAA 7 cut(s) 26, 393, 488, 640, 899, 1043, 1087
AhlI ACTAGT 1 cut(s) 817
AjiI CACGTC 1 cut(s) 402
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AloI GAACNNNNNNTCC 2 cut(s) 126, 158
AluBI AGCT 6 cut(s) 83, 115, 166, 545, 674, 932
AluI AGCT 6 cut(s) 83, 115, 166, 545, 674, 932
Alw26I GTCTC 2 cut(s) 505, 670
AlwI GGATC 1 cut(s) 919
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 5 cut(s) 365, 368, 371, 710, 713
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AseI ATTAAT 1 cut(s) 594
AspA2I CCTAGG 1 cut(s) 439
AspLEI GCGC 2 cut(s) 710, 1111
AsuHPI GGTGA 1 cut(s) 604
AsuII TTCGAA 2 cut(s) 175, 1170
AvrII CCTAGG 1 cut(s) 439
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 396
BbvI GCAGC 5 cut(s) 352, 355, 358, 697, 700
BccI CCATC 2 cut(s) 153, 880
BcgI CGANNNNNNTGC 2 cut(s) 1150, 1184
BciVI GTATCC 1 cut(s) 340
BcoDI GTCTC 2 cut(s) 505, 670
BcuI ACTAGT 1 cut(s) 817
BfaI CTAG 6 cut(s) 419, 440, 818, 855, 881, 929
BfoI RGCGCY 2 cut(s) 711, 1112
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 5 cut(s) 366, 369, 372, 711, 714
BlnI CCTAGG 1 cut(s) 439
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 5 cut(s) 367, 370, 373, 712, 715
BmcAI AGTACT 2 cut(s) 358, 1177
BmgBI CACGTC 1 cut(s) 402
BmiI GGNNCC 1 cut(s) 78
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 1 cut(s) 1065
BmuI ACTGGG 1 cut(s) 739
BpiI GAAGAC 1 cut(s) 396
Bpu1102I GCTNAGC 1 cut(s) 12
Bpu14I TTCGAA 2 cut(s) 175, 1170
BpuEI CTTGAG 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 439, 1035
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 745
Bse3DI GCAATG 2 cut(s) 1015, 1156
BseDI CCNNGG 2 cut(s) 439, 1035
BseGI GGATG 2 cut(s) 895, 1080
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMI GCAATG 2 cut(s) 1015, 1156
BseMII CTCAG 2 cut(s) 26, 102
BseNI ACTGG 1 cut(s) 745
BseXI GCAGC 5 cut(s) 352, 355, 358, 697, 700
BseYI CCCAGC 1 cut(s) 716
BsgI GTGCAG 1 cut(s) 990
BshFI GGCC 1 cut(s) 210
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 424
BsmAI GTCTC 2 cut(s) 505, 670
BsmFI GGGAC 1 cut(s) 330
BsnI GGCC 1 cut(s) 210
Bsp119I TTCGAA 2 cut(s) 175, 1170
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 1 cut(s) 911
Bsp1720I GCTNAGC 1 cut(s) 12
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 2 cut(s) 25, 103
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 1 cut(s) 919
BspT104I TTCGAA 2 cut(s) 175, 1170
BsrDI GCAATG 2 cut(s) 1015, 1156
BsrI ACTGG 1 cut(s) 745
BssECI CCNNGG 2 cut(s) 439, 1035
BssMI GATC 1 cut(s) 911
BssT1I CCWWGG 1 cut(s) 439
Bst6I CTCTTC 1 cut(s) 1007
BstAPI GCANNNNNTGC 1 cut(s) 830
BstBI TTCGAA 2 cut(s) 175, 1170
BstDEI CTNAG 3 cut(s) 12, 111, 675
BstDSI CCRYGG 1 cut(s) 1035
BstF5I GGATG 2 cut(s) 895, 1080
BstH2I RGCGCY 2 cut(s) 711, 1112
BstHHI GCGC 2 cut(s) 710, 1111
BstKTI GATC 1 cut(s) 914
BstMAI GTCTC 2 cut(s) 505, 670
BstMBI GATC 1 cut(s) 911
BstMWI GCNNNNNNNGC 3 cut(s) 371, 705, 830
BstNSI RCATGY 1 cut(s) 831
BstV1I GCAGC 5 cut(s) 352, 355, 358, 697, 700
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 1 cut(s) 911
BstYI RGATCY 1 cut(s) 911
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 1035
BtrI CACGTC 1 cut(s) 402
BtsCI GGATG 2 cut(s) 895, 1080
BtsI GCAGTG 2 cut(s) 795, 978
BtsIMutI CAGTG 2 cut(s) 795, 978
CfoI GCGC 2 cut(s) 710, 1111
Csp6I GTAC 3 cut(s) 357, 679, 1176
CviAII CATG 4 cut(s) 828, 1067, 1079, 1194
CviQI GTAC 3 cut(s) 357, 679, 1176
DdeI CTNAG 3 cut(s) 12, 111, 675
DpnI GATC 1 cut(s) 913
DpnII GATC 1 cut(s) 911
Eam1104I CTCTTC 1 cut(s) 1007
EarI CTCTTC 1 cut(s) 1007
Eco130I CCWWGG 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 988
EcoT14I CCWWGG 1 cut(s) 439
EcoT22I ATGCAT 2 cut(s) 1080, 1195
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 439
FaeI CATG 4 cut(s) 831, 1070, 1082, 1197
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 1 cut(s) 330
FatI CATG 4 cut(s) 827, 1066, 1078, 1193
FauNDI CATATG 2 cut(s) 695, 1129
Fnu4HI GCNGC 5 cut(s) 366, 369, 372, 711, 714
FokI GGATG 2 cut(s) 902, 1087
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 5 cut(s) 366, 369, 372, 711, 714
FspBI CTAG 6 cut(s) 419, 440, 818, 855, 881, 929
GlaI GCGC 2 cut(s) 709, 1110
GluI GCNGC 5 cut(s) 366, 369, 372, 711, 714
GsaI CCCAGC 1 cut(s) 720
HaeII RGCGCY 2 cut(s) 711, 1112
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 207
HhaI GCGC 2 cut(s) 710, 1111
Hin1II CATG 4 cut(s) 831, 1070, 1082, 1197
Hin6I GCGC 2 cut(s) 708, 1109
HinP1I GCGC 2 cut(s) 708, 1109
HincII GTYRAC 2 cut(s) 411, 924
HindII GTYRAC 2 cut(s) 411, 924
HinfI GANTC 7 cut(s) 237, 459, 537, 647, 690, 812, 925
HpaI GTTAAC 1 cut(s) 411
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 3 cut(s) 411, 579, 924
Hpy188I TCNGA 2 cut(s) 689, 845
Hpy188III TCNNGA 7 cut(s) 86, 247, 469, 757, 769, 881, 1148
Hpy8I GTNNAC 3 cut(s) 411, 579, 924
HpyAV CCTTC 4 cut(s) 92, 503, 763, 862
HpyCH4IV ACGT 2 cut(s) 401, 506
HpyCH4V TGCA 6 cut(s) 286, 699, 971, 1078, 1161, 1193
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 705, 830
HpyF3I CTNAG 3 cut(s) 12, 111, 675
HpySE526I ACGT 2 cut(s) 401, 506
Hsp92II CATG 4 cut(s) 831, 1070, 1082, 1197
HspAI GCGC 2 cut(s) 708, 1109
KspAI GTTAAC 1 cut(s) 411
Kzo9I GATC 1 cut(s) 911
Lsp1109I GCAGC 5 cut(s) 352, 355, 358, 697, 700
LweI GCATC 1 cut(s) 1065
MaeI CTAG 6 cut(s) 419, 440, 818, 855, 881, 929
MaeII ACGT 2 cut(s) 401, 506
MaeIII GTNAC 2 cut(s) 433, 813
MalI GATC 1 cut(s) 913
MboI GATC 1 cut(s) 911
MboII GAAGA 7 cut(s) 165, 246, 316, 396, 987, 994, 1099
MfeI CAATTG 1 cut(s) 530
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 6 cut(s) 63, 157, 339, 530, 595, 1017
MlyI GAGTC 3 cut(s) 641, 821, 919
MmeI TCCRAC 2 cut(s) 667, 823
MnlI CCTC 6 cut(s) 212, 317, 343, 631, 982, 1010
Mph1103I ATGCAT 2 cut(s) 1080, 1195
MseI TTAA 5 cut(s) 62, 410, 594, 740, 875
MspI CCGG 1 cut(s) 207
MunI CAATTG 1 cut(s) 530
MwoI GCNNNNNNNGC 3 cut(s) 371, 705, 830
NdeI CATATG 2 cut(s) 695, 1129
NdeII GATC 1 cut(s) 911
NlaIII CATG 4 cut(s) 831, 1070, 1082, 1197
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 813
NsiI ATGCAT 2 cut(s) 1080, 1195
NspI RCATGY 1 cut(s) 831
NspV TTCGAA 2 cut(s) 175, 1170
PciI ACATGT 1 cut(s) 827
PfeI GAWTC 4 cut(s) 237, 459, 537, 690
PkrI GCNGC 5 cut(s) 367, 370, 373, 712, 715
PleI GAGTC 3 cut(s) 641, 820, 919
PpsI GAGTC 3 cut(s) 641, 820, 919
PscI ACATGT 1 cut(s) 827
PshBI ATTAAT 1 cut(s) 594
PsiI TTATAA 1 cut(s) 1016
PspFI CCCAGC 1 cut(s) 716
PspN4I GGNNCC 1 cut(s) 78
PsuI RGATCY 1 cut(s) 911
RsaI GTAC 3 cut(s) 358, 680, 1177
RsaNI GTAC 3 cut(s) 357, 679, 1176
SaqAI TTAA 5 cut(s) 62, 410, 594, 740, 875
SatI GCNGC 5 cut(s) 366, 369, 372, 711, 714
Sau3AI GATC 1 cut(s) 911
ScaI AGTACT 2 cut(s) 358, 1177
SchI GAGTC 3 cut(s) 641, 821, 919
SduI GDGCHC 1 cut(s) 280
SfaNI GCATC 1 cut(s) 1065
SfuI TTCGAA 2 cut(s) 175, 1170
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SpeI ACTAGT 1 cut(s) 817
Sse9I AATT 6 cut(s) 63, 157, 339, 530, 595, 1017
SspI AATATT 1 cut(s) 389
SspMI CTAG 6 cut(s) 419, 440, 818, 855, 881, 929
StyI CCWWGG 1 cut(s) 439
TaiI ACGT 2 cut(s) 404, 509
TaqI TCGA 4 cut(s) 151, 175, 246, 1170
TasI AATT 6 cut(s) 63, 157, 339, 530, 595, 1017
TatI WGTACW 2 cut(s) 356, 1175
TfiI GAWTC 4 cut(s) 237, 459, 537, 690
Tru1I TTAA 5 cut(s) 62, 410, 594, 740, 875
Tru9I TTAA 5 cut(s) 62, 410, 594, 740, 875
TscAI CASTG 2 cut(s) 795, 978
TseFI GTSAC 1 cut(s) 813
TseI GCWGC 5 cut(s) 365, 368, 371, 710, 713
Tsp45I GTSAC 1 cut(s) 813
TspDTI ATGAA 4 cut(s) 465, 682, 1111, 1116
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 2 cut(s) 795, 978
VspI ATTAAT 1 cut(s) 594
XbaI TCTAGA 1 cut(s) 880
XceI RCATGY 1 cut(s) 831
XmaJI CCTAGG 1 cut(s) 439
XspI CTAG 6 cut(s) 419, 440, 818, 855, 881, 929
ZrmI AGTACT 2 cut(s) 358, 1177
Zsp2I ATGCAT 2 cut(s) 1080, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.