Rh7DG261100

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0018716
Physical Location & Seq
Forward (+)
27522016 .. 27537758
15743 bp
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UTR
Exon/CDS
Intron
Rh7DG261100.1

Sequence Viewer

Length: 624 bp
ATGGCAGAAGCTCAGCAAGAAGTTCAGCTTGGCATAACTCAAGATAACAAAGAAGGAAACTCAACTGAGCTAACAAGTGCCAACCACGAACCAACAATCCATCATCGAAGAAATTACAAGCTGTGGTTTCGAATAGCAACCTATTTATTCTTTGCCCTCTCCGGCCAATCAACAGCAACACTTCTGGGAAGATTCTACTTCGAGAAAGTGGGATTGATATTTGAGGTTTCTTCCCTCTTCTGCAATGTTATAATTGCTTTGGGTTTGCCCGTGGTTCAAGTTTTGGCTGTGATTGTTTTCCATGACAGGATGCATGGTTTGAAGATTGTGGCAATAGTTTTGGCCCTCTGGGGTTTCATTTCATATGTCTATCAGCAGTATCTTGATGACCATTGCAAATCATTCGAAAGGTTCATGGATATTCGAAACAAGCTGACCTATTCAGAAGGTATCCCGTTTCGCAGTCACTCTCAGTTCGTCACCGTGCCTCCAGCGTCGGGCCTCCTTGAGCGGCGCTCCTCCGCTTCGCTGTGCCACCACGACTGTCTCTCTATCTGCGCGACTCTGCCTCAGTCTCCCTCTGTTCCACAGCGCCACGATGACGGACTAATGGTGCTCTTTTAG

Protein Analysis

207

Amino Acids

23.48

Weight (kDa)

6.78

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 70 - 130 1.5e-22 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18190
rosa_chinensis RchiOBHm_Chr7g0210311
rosa_laevigata RLG00000003050
rosa_multiflora Rmu_sc0004503.1_g000004
rosa_roxburghii Rroxscaffold_3G00248440
rosa_samantha Rh7AG253500 Rh7CG270400 Rh7DG261100
rosa_wichuraiana Rw7G021610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AccBSI CCGCTC 1 cut(s) 511
AccII CGCG 1 cut(s) 560
AciI CCGC 2 cut(s) 511, 522
AcoI YGGCCR 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 2 cut(s) 278, 322
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
AluBI AGCT 5 cut(s) 11, 28, 70, 121, 433
AluI AGCT 5 cut(s) 11, 28, 70, 121, 433
Alw21I GWGCWC 1 cut(s) 618
Alw26I GTCTC 2 cut(s) 551, 579
AoxI GGCC 3 cut(s) 163, 342, 499
AspLEI GCGC 3 cut(s) 516, 560, 594
AspS9I GGNCC 2 cut(s) 343, 499
AsuHPI GGTGA 1 cut(s) 472
AsuII TTCGAA 3 cut(s) 130, 405, 424
Bbv12I GWGCWC 1 cut(s) 618
BccI CCATC 1 cut(s) 108
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciVI GTATCC 1 cut(s) 461
BcoDI GTCTC 2 cut(s) 551, 579
BfoI RGCGCY 2 cut(s) 517, 595
BfuI GTATCC 1 cut(s) 461
BisI GCNGC 1 cut(s) 512
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 513
BmgT120I GGNCC 2 cut(s) 343, 499
BmsI GCATC 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpmI CTGGAG 1 cut(s) 474
Bpu1102I GCTNAGC 1 cut(s) 12
Bpu14I TTCGAA 3 cut(s) 130, 405, 424
BpuEI CTTGAG 2 cut(s) 24, 527
BsaJI CCNNGG 1 cut(s) 270
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse3DI GCAATG 2 cut(s) 250, 391
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMI GCAATG 2 cut(s) 250, 391
BseMII CTCAG 4 cut(s) 26, 57, 485, 584
BseRI GAGGAG 1 cut(s) 508
Bsh1236I CGCG 1 cut(s) 560
BshFI GGCC 3 cut(s) 165, 344, 501
BsiHKAI GWGCWC 1 cut(s) 618
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 497
BsmAI GTCTC 2 cut(s) 551, 579
BsnI GGCC 3 cut(s) 165, 344, 501
Bsp119I TTCGAA 3 cut(s) 130, 405, 424
Bsp1286I GDGCHC 1 cut(s) 618
Bsp1720I GCTNAGC 1 cut(s) 12
BspACI CCGC 2 cut(s) 511, 522
BspANI GGCC 3 cut(s) 165, 344, 501
BspCNI CTCAG 4 cut(s) 25, 58, 484, 583
BspFNI CGCG 1 cut(s) 560
BspT104I TTCGAA 3 cut(s) 130, 405, 424
BsrBI CCGCTC 1 cut(s) 511
BsrDI GCAATG 2 cut(s) 250, 391
BssECI CCNNGG 1 cut(s) 270
Bst4CI ACNGT 2 cut(s) 484, 545
Bst6I CTCTTC 1 cut(s) 242
BstBI TTCGAA 3 cut(s) 130, 405, 424
BstDEI CTNAG 4 cut(s) 12, 66, 471, 570
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 1 cut(s) 315
BstFNI CGCG 1 cut(s) 560
BstH2I RGCGCY 2 cut(s) 517, 595
BstHHI GCGC 3 cut(s) 516, 560, 594
BstMAI GTCTC 2 cut(s) 551, 579
BstUI CGCG 1 cut(s) 560
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 3 cut(s) 165, 344, 501
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 1 cut(s) 315
CfoI GCGC 3 cut(s) 516, 560, 594
Cfr13I GGNCC 2 cut(s) 343, 499
CseI GACGC 1 cut(s) 483
CviAII CATG 3 cut(s) 302, 314, 415
CviJI RGCY 9 cut(s) 11, 28, 70, 121, 165, 287, 344, 433, 501
CviKI_1 RGCY 9 cut(s) 11, 28, 70, 121, 165, 287, 344, 433, 501
DdeI CTNAG 4 cut(s) 12, 66, 471, 570
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 315
FaeI CATG 3 cut(s) 305, 317, 418
FaiI YATR 7 cut(s) 35, 251, 303, 315, 364, 366, 416
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FatI CATG 3 cut(s) 301, 313, 414
FauNDI CATATG 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 512
FokI GGATG 1 cut(s) 322
Fsp4HI GCNGC 1 cut(s) 512
GlaI GCGC 3 cut(s) 515, 559, 593
GluI GCNGC 1 cut(s) 512
GsuI CTGGAG 1 cut(s) 474
HaeII RGCGCY 2 cut(s) 517, 595
HaeIII GGCC 3 cut(s) 165, 344, 501
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 483
HhaI GCGC 3 cut(s) 516, 560, 594
Hin1II CATG 3 cut(s) 305, 317, 418
Hin6I GCGC 3 cut(s) 514, 558, 592
HinP1I GCGC 3 cut(s) 514, 558, 592
HinfI GANTC 2 cut(s) 192, 562
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 472
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 3 cut(s) 41, 202, 383
Hpy99I CGWCG 1 cut(s) 499
HpyAV CCTTC 2 cut(s) 47, 440
HpyCH4III ACNGT 2 cut(s) 484, 545
HpyCH4V TGCA 3 cut(s) 243, 313, 396
HpyF3I CTNAG 4 cut(s) 12, 66, 471, 570
Hsp92II CATG 3 cut(s) 305, 317, 418
HspAI GCGC 3 cut(s) 514, 558, 592
LmnI GCTCC 1 cut(s) 521
LpnPI CCDG 5 cut(s) 170, 175, 292, 334, 504
LweI GCATC 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 464, 478
MbiI CCGCTC 1 cut(s) 511
MboII GAAGA 5 cut(s) 120, 201, 222, 229, 334
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 2 cut(s) 112, 252
MlyI GAGTC 1 cut(s) 556
MnlI CCTC 9 cut(s) 167, 217, 245, 356, 498, 512, 529, 579, 589
Mph1103I ATGCAT 1 cut(s) 315
MspI CCGG 1 cut(s) 162
MvnI CGCG 1 cut(s) 560
NdeI CATATG 1 cut(s) 364
NlaIII CATG 3 cut(s) 305, 317, 418
NmuCI GTSAC 2 cut(s) 464, 478
NsiI ATGCAT 1 cut(s) 315
NspV TTCGAA 3 cut(s) 130, 405, 424
PfeI GAWTC 1 cut(s) 192
PkrI GCNGC 1 cut(s) 513
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
PsiI TTATAA 1 cut(s) 251
PspPI GGNCC 2 cut(s) 343, 499
SatI GCNGC 1 cut(s) 512
Sau96I GGNCC 2 cut(s) 343, 499
SchI GAGTC 1 cut(s) 556
SduI GDGCHC 1 cut(s) 618
SfaNI GCATC 1 cut(s) 300
SfuI TTCGAA 3 cut(s) 130, 405, 424
SmlI CTYRAG 2 cut(s) 39, 506
SmoI CTYRAG 2 cut(s) 39, 506
Sse9I AATT 2 cut(s) 112, 252
SsiI CCGC 2 cut(s) 511, 522
TaaI ACNGT 2 cut(s) 484, 545
TaqI TCGA 5 cut(s) 106, 130, 201, 405, 424
TasI AATT 2 cut(s) 112, 252
TauI GCSGC 1 cut(s) 514
TfiI GAWTC 1 cut(s) 192
TseFI GTSAC 2 cut(s) 464, 478
Tsp45I GTSAC 2 cut(s) 464, 478
TspDTI ATGAA 3 cut(s) 346, 351, 403
TspGWI ACGGA 1 cut(s) 618
Zsp2I ATGCAT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.