RchiOBHm_Chr7g0220591

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0016581
Physical Location & Seq
Reverse (-)
39728441 .. 39729694
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
ATGGCGTGTGAAGCCGGCGGGGGTGGGCAAGAGAACGCGTCGTCGGGTTTGGGATCGCCGAAGAAGAACAGGGATAGGATCAAGTTGCTGTGCAGCTATGGCGGGAAAATCCTACCTAGACCTTCCGACGGCATCCTAAAGTACGTTGGTGGCGAGACTCGTGTCCTTGCCTGGTCCCGCGACGCAACTTTTTCTGAATTGATGAACAAATTGAGTGCAGTAGTTGATGGGGACATGGTGTTGAAGTACCAGTTAATCCCTGAAGATCTGGATGCCTTGGTCTCTGTTCGAAGCGACGAAGATGTAAAGCACATGCTGGACGAATATGATAGACAAGAAAACCAAGGCATGAGTCCTAGGCTAAGGACCTTCTTGTTTCCATCCAAGCCAATCATGATAGAAAACAGCAGCCACTCATTGGAGCTAGAGCAGCGTTACATTGACGCCATCAATGGCATAGTCCGTTCATCGAAACAACAGCCACCCTCAATCATAGGCAGCCCTCACATCGCCGGCACCAGCAGCATTGGGAATTGCAGCAGCCCCATAGCCAGCTACAGCCCCCTTGCTAGCTTTTCCTCTGCTTGTTCTTCCCCCAAGTCAGGATCGCCCGAACACCATTTTATGGTGGTACAAGAAGCTAACCAAATATTACAAGCCAGTAGATTTTCGCTTCCTCCTGTTATGCATAAAGTGCAGAGCTCTCCCAGCCTTTCTGGTAATCTTAATCTTCAACAATACCACAACCTAAACTTTGCTCACCGTCACCACCAGGGTGGTGGTGGTGGATACCATTACCAACAACCCCAAAGTGGCCACTACCAAATCTCTAGAAGCCTATCAAGAAACTCGCACCCCCACAACCACAGTAGAGGTGGTCGAGGCAGTCCCACCCCGCCCCATATGTCTCCCAGGGGAAGATCCGAGTCAGGGAGCGTGAGGAGCTTGATGGGTCAGTCTCGTCATAATTCGGGTTGCAGTAGCCCGAGACAACAAAACCAAGGACCAAATGCAGGAGAAGGCAACGATTATTGTGATGATCCATATGGCTACAACAGGGTTGATAGAGCTGAGAGTCTTCCAACAAGTCCTCGACAATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

367

Amino Acids

40.0

Weight (kDa)

8.78

Isoelectric Point (pI)

75.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 46 - 125 1.5e-13 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49920
fragaria_vesca FvH4_5g28590
malus_domestica MD15G1436100.v1.1
prunus_persica Prupe.1G579900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220591
rosa_laevigata RLG00000002215
rosa_multiflora Rmu_ssc0000189.1_g000028
rosa_roxburghii Rroxscaffold_3G00239280
rosa_rugosa Rorug07G0195200
rosa_samantha Rh7AG336200 Rh7BG327300 Rh7CG353900 Rh7DG332500
rosa_wichuraiana Rw7G028680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 515
AccB7I CCANNNNNTGG 2 cut(s) 418, 625
AccII CGCG 2 cut(s) 38, 180
AciI CCGC 4 cut(s) 18, 102, 178, 896
AclWI GGATC 5 cut(s) 61, 86, 613, 915, 1034
AcoI YGGCCR 1 cut(s) 814
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 444
AfaI GTAC 3 cut(s) 143, 248, 633
AfiI CCNNNNNNNGG 7 cut(s) 128, 418, 602, 625, 812, 930, 1013
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 2 cut(s) 244, 734
AjnI CCWGG 3 cut(s) 170, 771, 911
AleI CACNNNNGTG 1 cut(s) 774
AluBI AGCT 8 cut(s) 96, 424, 555, 573, 641, 702, 945, 1070
AluI AGCT 8 cut(s) 96, 424, 555, 573, 641, 702, 945, 1070
Alw21I GWGCWC 1 cut(s) 704
Alw26I GTCTC 5 cut(s) 149, 286, 912, 963, 982
AlwI GGATC 5 cut(s) 61, 86, 613, 915, 1034
Ama87I CYCGRG 1 cut(s) 985
AoxI GGCC 1 cut(s) 814
ApeKI GCWGC 7 cut(s) 93, 408, 430, 498, 522, 537, 540
AspA2I CCTAGG 1 cut(s) 356
AspS9I GGNCC 3 cut(s) 174, 366, 1004
AsuHPI GGTGA 2 cut(s) 752, 758
AsuII TTCGAA 1 cut(s) 289
AsuNHI GCTAGC 1 cut(s) 569
AvaI CYCGRG 1 cut(s) 985
AvaII GGWCC 3 cut(s) 174, 366, 1004
AvrII CCTAGG 1 cut(s) 356
BalI TGGCCA 1 cut(s) 816
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 704
BauI CACGAG 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 1070
Bbv12I GWGCWC 1 cut(s) 704
BbvI GCAGC 7 cut(s) 105, 420, 442, 510, 534, 549, 552
BccI CCATC 4 cut(s) 221, 388, 455, 943
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 3 cut(s) 172, 773, 913
BciVI GTATCC 1 cut(s) 782
BcoDI GTCTC 5 cut(s) 149, 286, 912, 963, 982
BfaI CTAG 5 cut(s) 117, 357, 425, 570, 831
BfmI CTRYAG 1 cut(s) 556
BfuI GTATCC 1 cut(s) 782
BglII AGATCT 1 cut(s) 265
BisI GCNGC 7 cut(s) 94, 409, 431, 499, 523, 538, 541
BlnI CCTAGG 1 cut(s) 356
BlsI GCNGC 7 cut(s) 95, 410, 432, 500, 524, 539, 542
Bme1390I CCNGG 3 cut(s) 172, 773, 913
Bme18I GGWCC 3 cut(s) 174, 366, 1004
BmeT110I CYCGRG 1 cut(s) 985
BmgT120I GGNCC 3 cut(s) 174, 366, 1004
BmiI GGNNCC 2 cut(s) 176, 517
BmrFI CCNGG 3 cut(s) 172, 773, 913
BmsI GCATC 2 cut(s) 141, 262
BmtI GCTAGC 1 cut(s) 573
BoxI GACNNNNGTC 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 1070
Bpu10I CCTNAGC 1 cut(s) 362
Bpu14I TTCGAA 1 cut(s) 289
BsaHI GRCGYC 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 7 cut(s) 276, 343, 356, 772, 911, 912, 1000
Bsc4I CCNNNNNNNGG 7 cut(s) 128, 418, 602, 625, 812, 930, 1013
Bse118I RCCGGY 2 cut(s) 14, 512
Bse1I ACTGG 2 cut(s) 250, 660
BseBI CCWGG 3 cut(s) 172, 773, 913
BseDI CCNNGG 7 cut(s) 276, 343, 356, 772, 911, 912, 1000
BseGI GGATG 3 cut(s) 132, 277, 380
BseLI CCNNNNNNNGG 7 cut(s) 128, 418, 602, 625, 812, 930, 1013
BseMII CTCAG 1 cut(s) 1062
BseNI ACTGG 2 cut(s) 250, 660
BseRI GAGGAG 1 cut(s) 955
BseXI GCAGC 7 cut(s) 105, 420, 442, 510, 534, 549, 552
BseYI CCCAGC 1 cut(s) 707
BsgI GTGCAG 3 cut(s) 112, 237, 716
Bsh1236I CGCG 2 cut(s) 38, 180
BshFI GGCC 1 cut(s) 816
BshNI GGYRCC 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 704
BsiHKCI CYCGRG 1 cut(s) 985
BsiSI CCGG 2 cut(s) 15, 513
BslFI GGGAC 3 cut(s) 160, 245, 873
BslI CCNNNNNNNGG 7 cut(s) 128, 418, 602, 625, 812, 930, 1013
BsmAI GTCTC 5 cut(s) 149, 286, 912, 963, 982
BsmFI GGGAC 3 cut(s) 160, 245, 873
BsnI GGCC 1 cut(s) 816
Bso31I GGTCTC 1 cut(s) 286
BsoBI CYCGRG 1 cut(s) 985
Bsp119I TTCGAA 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 704
Bsp143I GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
BspACI CCGC 4 cut(s) 18, 102, 178, 896
BspANI GGCC 1 cut(s) 816
BspCNI CTCAG 1 cut(s) 1063
BspFNI CGCG 2 cut(s) 38, 180
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 176, 517
BspOI GCTAGC 1 cut(s) 573
BspPI GGATC 5 cut(s) 61, 86, 613, 915, 1034
BspT104I TTCGAA 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 286
BsrFI RCCGGY 2 cut(s) 14, 512
BsrI ACTGG 2 cut(s) 250, 660
BssAI RCCGGY 2 cut(s) 14, 512
BssECI CCNNGG 7 cut(s) 276, 343, 356, 772, 911, 912, 1000
BssMI GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
BssNI GRCGYC 1 cut(s) 444
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 4 cut(s) 276, 343, 356, 1000
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 3 cut(s) 172, 773, 913
Bst4CI ACNGT 2 cut(s) 764, 869
BstACI GRCGYC 1 cut(s) 444
BstAPI GCANNNNNTGC 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 289
BstC8I GCNNGC 4 cut(s) 16, 514, 553, 571
BstDEI CTNAG 2 cut(s) 362, 1071
BstF5I GGATG 3 cut(s) 132, 277, 380
BstFNI CGCG 2 cut(s) 38, 180
BstKTI GATC 6 cut(s) 56, 81, 268, 608, 923, 1042
BstMAI GTCTC 5 cut(s) 149, 286, 912, 963, 982
BstMBI GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
BstMWI GCNNNNNNNGC 7 cut(s) 11, 99, 430, 522, 694, 708, 942
BstNI CCWGG 3 cut(s) 172, 773, 913
BstNSI RCATGY 1 cut(s) 316
BstPAI GACNNNNGTC 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 170, 771, 911
BstSFI CTRYAG 1 cut(s) 556
BstUI CGCG 2 cut(s) 38, 180
BstV1I GCAGC 7 cut(s) 105, 420, 442, 510, 534, 549, 552
BstV2I GAAGAC 1 cut(s) 1070
BstX2I RGATCY 2 cut(s) 265, 920
BstXI CCANNNNNNTGG 2 cut(s) 776, 779
BstYI RGATCY 2 cut(s) 265, 920
BsuI GTATCC 1 cut(s) 782
BsuRI GGCC 1 cut(s) 816
BtgZI GCGATG 1 cut(s) 493
BtsCI GGATG 3 cut(s) 132, 277, 380
Cac8I GCNNGC 4 cut(s) 16, 514, 553, 571
CciI TCATGA 1 cut(s) 393
Cfr10I RCCGGY 2 cut(s) 14, 512
Cfr13I GGNCC 3 cut(s) 174, 366, 1004
CseI GACGC 3 cut(s) 27, 191, 452
Csp6I GTAC 3 cut(s) 142, 247, 632
CviAII CATG 4 cut(s) 235, 313, 349, 394
CviQI GTAC 3 cut(s) 142, 247, 632
DdeI CTNAG 2 cut(s) 362, 1071
DpnI GATC 6 cut(s) 55, 80, 267, 607, 922, 1041
DpnII GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
EaeI YGGCCR 1 cut(s) 814
Ecl136II GAGCTC 1 cut(s) 702
Eco130I CCWWGG 4 cut(s) 276, 343, 356, 1000
Eco24I GRGCYC 1 cut(s) 704
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 3 cut(s) 174, 366, 1004
Eco53kI GAGCTC 1 cut(s) 702
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 985
EcoICRI GAGCTC 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 366
EcoRII CCWGG 3 cut(s) 170, 771, 911
EcoT14I CCWWGG 4 cut(s) 276, 343, 356, 1000
EcoT22I ATGCAT 1 cut(s) 690
EcoT38I GRGCYC 1 cut(s) 704
ErhI CCWWGG 4 cut(s) 276, 343, 356, 1000
FaeI CATG 4 cut(s) 238, 316, 352, 397
FaqI GGGAC 3 cut(s) 160, 245, 873
FatI CATG 4 cut(s) 234, 312, 348, 393
FauI CCCGC 4 cut(s) 11, 95, 185, 903
FauNDI CATATG 2 cut(s) 903, 1045
Fnu4HI GCNGC 7 cut(s) 94, 409, 431, 499, 523, 538, 541
FokI GGATG 3 cut(s) 119, 284, 367
FriOI GRGCYC 1 cut(s) 704
Fsp4HI GCNGC 7 cut(s) 94, 409, 431, 499, 523, 538, 541
FspBI CTAG 5 cut(s) 117, 357, 425, 570, 831
GluI GCNGC 7 cut(s) 94, 409, 431, 499, 523, 538, 541
GsaI CCCAGC 1 cut(s) 711
HaeIII GGCC 1 cut(s) 816
HapII CCGG 2 cut(s) 15, 513
HgaI GACGC 3 cut(s) 27, 191, 452
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 4 cut(s) 238, 316, 352, 397
HinfI GANTC 4 cut(s) 157, 352, 926, 1075
HpaII CCGG 2 cut(s) 15, 513
HphI GGTGA 2 cut(s) 752, 758
Hpy188I TCNGA 3 cut(s) 127, 196, 925
Hpy188III TCNNGA 5 cut(s) 269, 394, 603, 831, 843
Hpy99I CGWCG 5 cut(s) 43, 46, 131, 185, 299
HpyAV CCTTC 3 cut(s) 132, 379, 1013
HpyCH4III ACNGT 2 cut(s) 764, 869
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 7 cut(s) 93, 218, 537, 688, 697, 978, 1013
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 99, 430, 522, 694, 708, 942
HpyF3I CTNAG 2 cut(s) 362, 1071
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 4 cut(s) 238, 316, 352, 397
KroI GCCGGC 2 cut(s) 14, 512
KroNI GCCGGC 2 cut(s) 16, 514
Kzo9I GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
LmnI GCTCC 3 cut(s) 421, 933, 942
Lsp1109I GCAGC 7 cut(s) 105, 420, 442, 510, 534, 549, 552
LweI GCATC 2 cut(s) 141, 262
MaeI CTAG 5 cut(s) 117, 357, 425, 570, 831
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 434, 764
MalI GATC 6 cut(s) 55, 80, 267, 607, 922, 1041
MboI GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
MboII GAAGA 8 cut(s) 73, 76, 275, 311, 582, 722, 930, 1070
MflI RGATCY 2 cut(s) 265, 920
MhlI GDGCHC 1 cut(s) 704
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 4 cut(s) 197, 209, 532, 967
MluI ACGCGT 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 4 cut(s) 151, 361, 935, 1084
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 496, 513, 589, 687, 866, 875, 933, 1101
Mox20I TGGCCA 1 cut(s) 816
Mph1103I ATGCAT 1 cut(s) 690
MroNI GCCGGC 2 cut(s) 14, 512
MscI TGGCCA 1 cut(s) 816
MseI TTAA 2 cut(s) 254, 726
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 816
MspI CCGG 2 cut(s) 15, 513
MspR9I CCNGG 3 cut(s) 172, 773, 913
MvaI CCWGG 3 cut(s) 172, 773, 913
MvnI CGCG 2 cut(s) 38, 180
MwoI GCNNNNNNNGC 7 cut(s) 11, 99, 430, 522, 694, 708, 942
NaeI GCCGGC 2 cut(s) 16, 514
NdeI CATATG 2 cut(s) 903, 1045
NdeII GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
NgoMIV GCCGGC 2 cut(s) 14, 512
NheI GCTAGC 1 cut(s) 569
NlaIII CATG 4 cut(s) 238, 316, 352, 397
NlaIV GGNNCC 2 cut(s) 176, 517
NmuCI GTSAC 1 cut(s) 764
NsiI ATGCAT 1 cut(s) 690
NspI RCATGY 1 cut(s) 316
NspV TTCGAA 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 774
PagI TCATGA 1 cut(s) 393
PasI CCCWGGG 1 cut(s) 912
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdiI GCCGGC 2 cut(s) 16, 514
PflMI CCANNNNNTGG 2 cut(s) 418, 625
PkrI GCNGC 7 cut(s) 95, 410, 432, 500, 524, 539, 542
PleI GAGTC 4 cut(s) 151, 360, 934, 1083
PpsI GAGTC 4 cut(s) 151, 360, 934, 1083
PpuMI RGGWCCY 1 cut(s) 366
PshAI GACNNNNGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 704
Psp5II RGGWCCY 1 cut(s) 366
Psp6I CCWGG 3 cut(s) 170, 771, 911
PspFI CCCAGC 1 cut(s) 707
PspGI CCWGG 3 cut(s) 170, 771, 911
PspN4I GGNNCC 2 cut(s) 176, 517
PspPI GGNCC 3 cut(s) 174, 366, 1004
PspPPI RGGWCCY 1 cut(s) 366
PsuI RGATCY 2 cut(s) 265, 920
RsaI GTAC 3 cut(s) 143, 248, 633
RsaNI GTAC 3 cut(s) 142, 247, 632
RseI CAYNNNNRTG 1 cut(s) 774
SacI GAGCTC 1 cut(s) 704
SaqAI TTAA 2 cut(s) 254, 726
SatI GCNGC 7 cut(s) 94, 409, 431, 499, 523, 538, 541
Sau3AI GATC 6 cut(s) 53, 78, 265, 605, 920, 1039
Sau96I GGNCC 3 cut(s) 174, 366, 1004
SchI GAGTC 4 cut(s) 151, 361, 935, 1084
ScrFI CCNGG 3 cut(s) 172, 773, 913
SduI GDGCHC 1 cut(s) 704
SfaNI GCATC 2 cut(s) 141, 262
SfcI CTRYAG 1 cut(s) 556
SfuI TTCGAA 1 cut(s) 289
SinI GGWCC 3 cut(s) 174, 366, 1004
SmiMI CAYNNNNRTG 1 cut(s) 774
Sse9I AATT 4 cut(s) 197, 209, 532, 967
SsiI CCGC 4 cut(s) 18, 102, 178, 896
SspI AATATT 1 cut(s) 651
SspMI CTAG 5 cut(s) 117, 357, 425, 570, 831
SstI GAGCTC 1 cut(s) 704
StyD4I CCNGG 3 cut(s) 170, 771, 911
StyI CCWWGG 4 cut(s) 276, 343, 356, 1000
TaaI ACNGT 2 cut(s) 764, 869
TaiI ACGT 1 cut(s) 147
TaqI TCGA 4 cut(s) 289, 470, 880, 1093
TasI AATT 4 cut(s) 197, 209, 532, 967
Tru1I TTAA 2 cut(s) 254, 726
Tru9I TTAA 2 cut(s) 254, 726
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 7 cut(s) 93, 408, 430, 498, 522, 537, 540
Tsp45I GTSAC 1 cut(s) 764
TspDTI ATGAA 2 cut(s) 218, 456
TspGWI ACGGA 1 cut(s) 452
Van91I CCANNNNNTGG 2 cut(s) 418, 625
VpaK11BI GGWCC 3 cut(s) 174, 366, 1004
XbaI TCTAGA 1 cut(s) 830
XceI RCATGY 1 cut(s) 316
XcmI CCANNNNNNNNNTGG 3 cut(s) 776, 779, 872
XmaJI CCTAGG 1 cut(s) 356
XspI CTAG 5 cut(s) 117, 357, 425, 570, 831
Zsp2I ATGCAT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.