RchiOBHm_Chr5g0011711

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018812
Physical Location & Seq
Reverse (-)
7774305 .. 7776584
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGTTCATGCCTGTAAGCTGCTCAAACTGCAACACATATCTGCAGTTACCTACAGGTTCTCAGATTGTATGCTGCTCCAGATGCATATTGCGCTTTGCCTACTCAAGATGTCCTCCTCCTCCTCCTCCTCCTCGCCGGTCATACTCCTTCTCCTCTTCCTCCTACAACAACCGCCGTCCTTCCCTGTCATATGCCTTCTCCTTTTTCTCGTACAACAACCGCCCTCCTCCGCCTTCTCGGTTGCCTTGCCAACGCAAGCGAGCCGTGATATGTGGCATAAATTATAAGGGAATGGGGAAGAACATGGAGCTCGAGGGCTGCATTAATGATGCGATTGCAATGCAGAATTTGCTAGTCAGGGAGTTCAAATTCCCTGAGTCTTCCATTCGCATGCTCACTGAAAAAGGAGGTCATCAGCCTCCTACCAAAATAAATTTAACGATAGCACTGCGTTGGCTTGTGCAAGATTGTCAAGCAGGGGATTCGTTGGTGTTTTATTTTGCCGGTCATGGTGATCAACAGCCCAACAACACAGGAGACGAGGTGGACGAACTAGACGAGATACTTTTGCCCTTAGACTACGAGACTAAGGGAGTGATCGTTGATGATGAGATCAATGAAATGATTGTCAGACCTATTCCGGCCGGTGTCAAGCTCCATGCTATTGTGGATGCTTGCCACAGTGGTACTGTTCTTGATTTGCCATTTCTCTGCAAAATAGACAAAAGCGGCCAGAGGTGGTTCTGGAAGGACCAAAGACGACCTCAAAAAGCTGGTGTTTGGAAAGGAACGAAAGGTGGAGAGGTCATTTCCTTTAGTGGATGTAATGATCACCAGTGTGCTGTAGAGAAAACAATCCCACGGGGTGCCATGACTTGGGCTTTCGTCGATGCCATTGATCGTGGGTGTGGAAATACATATGGCAACTTGTTAATTGCAATGCGATCTACCGTCCGTAAGGATTGTATTGATACGGATTCCGCCATACAAGAACCACAACTAACTTCAAGTGAAGTATTTGATGTGTTCACAAGGCCTTTCTCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010941 GO:0043067 GO:0043069 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14 zf-LSD1

Protein Analysis

349

Amino Acids

39.09

Weight (kDa)

8.1

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 85 - 342 3.3e-32 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018812)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07290
rosa_chinensis RchiOBHm_Chr5g0011711
rosa_laevigata RLG00000031857
rosa_multiflora Rmu_co8248297.1_g000001 Rmu_sc0007176.1_g000004
rosa_roxburghii Rroxscaffold_1G00064810
rosa_rugosa Rorug04G0458600
rosa_samantha Rh5DG085800
rosa_wichuraiana Rw5G007870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 876
AciI CCGC 5 cut(s) 172, 220, 230, 729, 981
AcoI YGGCCR 2 cut(s) 642, 730
AcsI RAATTY 3 cut(s) 346, 368, 433
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 2 cut(s) 212, 688
AfiI CCNNNNNNNGG 1 cut(s) 876
AgsI TTSAA 2 cut(s) 367, 1008
AleI CACNNNNGTG 1 cut(s) 837
AluBI AGCT 4 cut(s) 18, 310, 655, 773
AluI AGCT 4 cut(s) 18, 310, 655, 773
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 2 cut(s) 531, 578
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 3 cut(s) 642, 730, 1034
ApeKI GCWGC 3 cut(s) 18, 72, 318
ApoI RAATTY 3 cut(s) 346, 368, 433
AseI ATTAAT 1 cut(s) 324
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 751
AsuHPI GGTGA 2 cut(s) 524, 824
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 751
BanI GGYRCC 1 cut(s) 866
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 3 cut(s) 5, 59, 305
BceAI ACGGC 2 cut(s) 159, 248
BcgI CGANNNNNNTGC 6 cut(s) 322, 356, 430, 464, 465, 499
BclI TGATCA 2 cut(s) 514, 829
BcoDI GTCTC 2 cut(s) 531, 578
BfaI CTAG 2 cut(s) 353, 554
BfmI CTRYAG 4 cut(s) 41, 51, 843, 1046
BisI GCNGC 4 cut(s) 19, 73, 319, 730
BlsI GCNGC 4 cut(s) 20, 74, 320, 731
Bme18I GGWCC 1 cut(s) 751
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 1 cut(s) 868
BmsI GCATC 4 cut(s) 71, 319, 661, 880
BpiI GAAGAC 1 cut(s) 372
BpmI CTGGAG 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 860
BsaXI ACNNNNNCTCC 6 cut(s) 134, 164, 528, 558, 585, 615
Bsc4I CCNNNNNNNGG 1 cut(s) 876
Bse118I RCCGGY 3 cut(s) 135, 503, 644
Bse1I ACTGG 1 cut(s) 835
Bse3DI GCAATG 2 cut(s) 345, 945
BseDI CCNNGG 1 cut(s) 860
BseGI GGATG 2 cut(s) 676, 827
BseLI CCNNNNNNNGG 1 cut(s) 876
BseMI GCAATG 2 cut(s) 345, 945
BseMII CTCAG 2 cut(s) 74, 366
BseNI ACTGG 1 cut(s) 835
BseRI GAGGAG 8 cut(s) 105, 108, 111, 114, 117, 120, 142, 216
BseX3I CGGCCG 1 cut(s) 642
BseXI GCAGC 3 cut(s) 5, 59, 305
Bsh1285I CGRYCG 1 cut(s) 645
BshFI GGCC 3 cut(s) 644, 732, 1036
BshNI GGYRCC 1 cut(s) 866
BsiEI CGRYCG 1 cut(s) 645
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 4 cut(s) 136, 504, 641, 645
BslI CCNNNNNNNGG 1 cut(s) 876
BsmAI GTCTC 2 cut(s) 531, 578
BsmBI CGTCTC 1 cut(s) 531
BsnI GGCC 3 cut(s) 644, 732, 1036
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 6 cut(s) 514, 597, 612, 829, 898, 944
BspACI CCGC 5 cut(s) 172, 220, 230, 729, 981
BspANI GGCC 3 cut(s) 644, 732, 1036
BspCNI CTCAG 2 cut(s) 73, 367
BspLI GGNNCC 1 cut(s) 868
BspMAI CTGCAG 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 866
BsrDI GCAATG 2 cut(s) 345, 945
BsrFI RCCGGY 3 cut(s) 135, 503, 644
BsrI ACTGG 1 cut(s) 835
BssAI RCCGGY 3 cut(s) 135, 503, 644
BssECI CCNNGG 1 cut(s) 860
BssMI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
Bst4CI ACNGT 3 cut(s) 683, 691, 952
Bst6I CTCTTC 1 cut(s) 160
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 4 cut(s) 257, 261, 392, 676
BstDEI CTNAG 4 cut(s) 60, 375, 574, 588
BstDSI CCRYGG 1 cut(s) 860
BstF5I GGATG 2 cut(s) 676, 827
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 6 cut(s) 517, 600, 615, 832, 901, 947
BstMAI GTCTC 2 cut(s) 531, 578
BstMBI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
BstMCI CGRYCG 1 cut(s) 645
BstMWI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 349
BstNSI RCATGY 1 cut(s) 394
BstSFI CTRYAG 4 cut(s) 41, 51, 843, 1046
BstV1I GCAGC 3 cut(s) 5, 59, 305
BstV2I GAAGAC 1 cut(s) 372
BstZI CGGCCG 1 cut(s) 642
BsuRI GGCC 3 cut(s) 644, 732, 1036
BtgI CCRYGG 1 cut(s) 860
BtsCI GGATG 2 cut(s) 676, 827
BtsI GCAGTG 1 cut(s) 446
BtsIMutI CAGTG 4 cut(s) 396, 446, 688, 842
Cac8I GCNNGC 4 cut(s) 257, 261, 392, 676
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 3 cut(s) 135, 503, 644
Cfr13I GGNCC 1 cut(s) 751
Csp6I GTAC 2 cut(s) 211, 687
CviAII CATG 6 cut(s) 7, 304, 391, 509, 659, 871
CviQI GTAC 2 cut(s) 211, 687
DdeI CTNAG 4 cut(s) 60, 375, 574, 588
DpnI GATC 6 cut(s) 516, 599, 614, 831, 900, 946
DpnII GATC 6 cut(s) 514, 597, 612, 829, 898, 944
DraIII CACNNNGTG 1 cut(s) 866
EaeI YGGCCR 2 cut(s) 642, 730
EagI CGGCCG 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
EciI GGCGGA 2 cut(s) 219, 970
Ecl136II GAGCTC 1 cut(s) 310
EclXI CGGCCG 1 cut(s) 642
Eco147I AGGCCT 1 cut(s) 1036
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 751
Eco52I CGGCCG 1 cut(s) 642
Eco53kI GAGCTC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 312
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 6 cut(s) 10, 307, 394, 512, 662, 874
FatI CATG 6 cut(s) 6, 303, 390, 508, 658, 870
FauNDI CATATG 2 cut(s) 190, 919
FbaI TGATCA 2 cut(s) 514, 829
Fnu4HI GCNGC 4 cut(s) 19, 73, 319, 730
FokI GGATG 2 cut(s) 683, 834
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 4 cut(s) 19, 73, 319, 730
FspBI CTAG 2 cut(s) 353, 554
GlaI GCGC 1 cut(s) 92
GluI GCNGC 4 cut(s) 19, 73, 319, 730
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 3 cut(s) 644, 732, 1036
HapII CCGG 4 cut(s) 136, 504, 641, 645
HhaI GCGC 1 cut(s) 93
Hin1II CATG 6 cut(s) 10, 307, 394, 512, 662, 874
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 3 cut(s) 377, 482, 977
HpaII CCGG 4 cut(s) 136, 504, 641, 645
HphI GGTGA 2 cut(s) 524, 824
Hpy166II GTNNAC 2 cut(s) 547, 1029
Hpy188I TCNGA 2 cut(s) 63, 632
Hpy188III TCNNGA 4 cut(s) 78, 105, 695, 745
Hpy8I GTNNAC 2 cut(s) 547, 1029
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 5 cut(s) 157, 189, 205, 243, 742
HpyCH4III ACNGT 3 cut(s) 683, 691, 952
HpyCH4V TGCA 9 cut(s) 30, 43, 84, 321, 338, 343, 463, 714, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 349
HpyF3I CTNAG 4 cut(s) 60, 375, 574, 588
Hsp92II CATG 6 cut(s) 10, 307, 394, 512, 662, 874
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 2 cut(s) 514, 829
Kzo9I GATC 6 cut(s) 514, 597, 612, 829, 898, 944
LmnI GCTCC 3 cut(s) 80, 307, 660
Lsp1109I GCAGC 3 cut(s) 5, 59, 305
LweI GCATC 4 cut(s) 71, 319, 661, 880
MaeI CTAG 2 cut(s) 353, 554
MaeIII GTNAC 1 cut(s) 45
MalI GATC 6 cut(s) 516, 599, 614, 831, 900, 946
MboI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
MboII GAAGA 3 cut(s) 147, 310, 372
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 5 cut(s) 280, 346, 368, 433, 933
MlyI GAGTC 1 cut(s) 386
Mph1103I ATGCAT 1 cut(s) 86
MseI TTAA 3 cut(s) 324, 437, 932
MslI CAYNNNNRTG 2 cut(s) 389, 837
MspI CCGG 4 cut(s) 136, 504, 641, 645
MwoI GCNNNNNNNGC 4 cut(s) 27, 81, 90, 349
NdeI CATATG 2 cut(s) 190, 919
NdeII GATC 6 cut(s) 514, 597, 612, 829, 898, 944
NlaIII CATG 6 cut(s) 10, 307, 394, 512, 662, 874
NlaIV GGNNCC 1 cut(s) 868
NsiI ATGCAT 1 cut(s) 86
NspI RCATGY 1 cut(s) 394
OliI CACNNNNGTG 1 cut(s) 837
PaeI GCATGC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 311
PceI AGGCCT 1 cut(s) 1036
PcsI WCGNNNNNNNCGW 2 cut(s) 546, 555
PfeI GAWTC 2 cut(s) 482, 977
PflMI CCANNNNNTGG 1 cut(s) 876
PkrI GCNGC 4 cut(s) 20, 74, 320, 731
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PshBI ATTAAT 1 cut(s) 324
PsiI TTATAA 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 868
PspPI GGNCC 1 cut(s) 751
PspXI VCTCGAGB 1 cut(s) 311
PstI CTGCAG 1 cut(s) 45
RsaI GTAC 2 cut(s) 212, 688
RsaNI GTAC 2 cut(s) 211, 687
RseI CAYNNNNRTG 2 cut(s) 389, 837
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 3 cut(s) 324, 437, 932
SatI GCNGC 4 cut(s) 19, 73, 319, 730
Sau3AI GATC 6 cut(s) 514, 597, 612, 829, 898, 944
Sau96I GGNCC 1 cut(s) 751
SchI GAGTC 1 cut(s) 386
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 4 cut(s) 71, 319, 661, 880
SfcI CTRYAG 4 cut(s) 41, 51, 843, 1046
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 1 cut(s) 751
SlaI CTCGAG 1 cut(s) 311
SmiMI CAYNNNNRTG 2 cut(s) 389, 837
SmlI CTYRAG 2 cut(s) 103, 311
SmoI CTYRAG 2 cut(s) 103, 311
SphI GCATGC 1 cut(s) 394
Sse9I AATT 5 cut(s) 280, 346, 368, 433, 933
SseBI AGGCCT 1 cut(s) 1036
SsiI CCGC 5 cut(s) 172, 220, 230, 729, 981
SspMI CTAG 2 cut(s) 353, 554
SstI GAGCTC 1 cut(s) 312
StuI AGGCCT 1 cut(s) 1036
TaaI ACNGT 3 cut(s) 683, 691, 952
TaqI TCGA 2 cut(s) 312, 888
TasI AATT 5 cut(s) 280, 346, 368, 433, 933
TauI GCSGC 1 cut(s) 732
TfiI GAWTC 2 cut(s) 482, 977
Tru1I TTAA 3 cut(s) 324, 437, 932
Tru9I TTAA 3 cut(s) 324, 437, 932
TscAI CASTG 4 cut(s) 403, 453, 688, 842
TseI GCWGC 3 cut(s) 18, 72, 318
TspDTI ATGAA 1 cut(s) 633
TspGWI ACGGA 2 cut(s) 944, 989
TspRI CASTG 4 cut(s) 403, 453, 688, 842
Van91I CCANNNNNTGG 1 cut(s) 876
VpaK11BI GGWCC 1 cut(s) 751
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 3 cut(s) 346, 368, 433
XceI RCATGY 1 cut(s) 394
XhoI CTCGAG 1 cut(s) 311
XspI CTAG 2 cut(s) 353, 554
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.