RchiOBHm_Chr5g0014071

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018392
Physical Location & Seq
Forward (+)
9562724 .. 9563736
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 930 bp
ATGGTTCTGCTCAGATTGCAGCTCTACAAGCTTACCGGGAGTGGTTGGATGGATGGAGACGCCTATGACGACGTTCCAGTTGCAAGAGATGTTACTTATGAAGAATCTCCCATCTGGACAGAGTCTCACTTTTGGATCAGCCTCTCATCTCTCAAGCACTTGCCGAAAAGGAAGAAGCACACTGAGCTTTTTACAGAGGTTCTAGAGGATTGGAGTGTGCCAAAACCTCAGCAGCCAGCTATTATTGCACAATTGTTTCAAGTGGTTGATGGAGCTTCCCCCAAGTTGTCCATCATTGTTGCCGTGCGCAGCCTCATTCTGCGGTTGCGCATTGTGAGTAGCTTTGGTTATGATGCAACGGAGCTCAATCCTACAGCTGAATCATCCACATTCGTTGAGGAGGGTTTGGAGAAAGTGGGCCTTGAAAACTCGGGAGCTACAGAACACAAGAAAACCTCTTCATGTGTTTTGTGCATGGAGGGCCTTTTCGAATCTGACAACGGTGCTCAACAACTTACTCGCTTGCCCTGCTCGCACTCTTATCATGGACGCTGCTTACTTCAGTGGCCATCAGAGATAAGTAGCTCGTGTCCCTTGTGCGAAAAGCCAAAGTCGAAATTTCATGCTTGTCGGCAATTTTGGGTTCGGATGGTGAGGAAGCACGTGGGTACTGTGATATCTGCTCTGATTTTTTGCCAATTTTTCAAGCCTACTCTTCAAGTCCAGCCTACTCTTCAAGTCCAGCCTTTAGAGACCTCCCCTGCCCGTCCTAAAAGCTGGAAGCAGAGTCTGCTTGAAATGGATGAAATGATGACCATGACGGCAACCAAATTGGTAAACGATTTCCAATCCGGCAAACTATTGAAGCAAATTAAGAAAGAATGGTGGAATTCAAGAAGGAATGCGAAACCAAATGTAGTCAGACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

309

Amino Acids

35.19

Weight (kDa)

8.83

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 154 - 200 5.3e-09 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018392)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05601
rosa_chinensis RchiOBHm_Chr5g0014071
rosa_laevigata RLG00000032079
rosa_multiflora Rmu_sc0007767.1_g000009
rosa_rugosa Rorug05G0015300 Rorug05G0015400
rosa_samantha Rh5AG109400 Rh5BG106200 Rh5DG104900
rosa_wichuraiana Rw5G009550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 308, 329
AciI CCGC 1 cut(s) 322
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 566
AcsI RAATTY 2 cut(s) 617, 889
AcuI CTGAAG 1 cut(s) 545
AcvI CACGTG 1 cut(s) 664
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 670
AgsI TTSAA 8 cut(s) 260, 425, 706, 719, 737, 797, 865, 894
Alw21I GWGCWC 2 cut(s) 366, 508
Alw26I GTCTC 3 cut(s) 51, 129, 746
AlwI GGATC 1 cut(s) 143
AlwNI CAGNNNCTG 1 cut(s) 790
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 3 cut(s) 418, 481, 566
ApeKI GCWGC 4 cut(s) 19, 232, 309, 552
ApoI RAATTY 2 cut(s) 617, 889
AspLEI GCGC 2 cut(s) 309, 330
AspS9I GGNCC 2 cut(s) 418, 481
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 664
AsuII TTCGAA 1 cut(s) 489
AvaI CYCGRG 1 cut(s) 430
BalI TGGCCA 1 cut(s) 568
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 586
BbrPI CACGTG 1 cut(s) 664
Bbv12I GWGCWC 2 cut(s) 366, 508
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 4 cut(s) 31, 244, 321, 539
BccI CCATC 7 cut(s) 43, 47, 119, 263, 299, 577, 643
BceAI ACGGC 2 cut(s) 287, 837
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 3 cut(s) 51, 129, 746
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 2 cut(s) 372, 438
BisI GCNGC 4 cut(s) 20, 233, 310, 553
BlsI GCNGC 4 cut(s) 21, 234, 311, 554
Bme1390I CCNGG 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 430
BmgT120I GGNCC 2 cut(s) 418, 481
BmrFI CCNGG 1 cut(s) 37
BmsI GCATC 1 cut(s) 343
Bpu10I CCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 37
BsaAI YACGTR 1 cut(s) 664
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 746
Bse1I ACTGG 1 cut(s) 77
BseGI GGATG 5 cut(s) 54, 58, 383, 654, 808
BseMII CTCAG 3 cut(s) 25, 174, 242
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 4 cut(s) 31, 244, 321, 539
BshFI GGCC 3 cut(s) 420, 483, 568
BsiHKAI GWGCWC 2 cut(s) 366, 508
BsiHKCI CYCGRG 1 cut(s) 430
BsiSI CCGG 2 cut(s) 36, 852
BslFI GGGAC 1 cut(s) 576
BsmAI GTCTC 3 cut(s) 51, 129, 746
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 3 cut(s) 420, 483, 568
Bso31I GGTCTC 1 cut(s) 746
BsoBI CYCGRG 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 489
Bsp1286I GDGCHC 2 cut(s) 366, 508
Bsp143I GATC 1 cut(s) 135
BspACI CCGC 1 cut(s) 322
BspANI GGCC 3 cut(s) 420, 483, 568
BspCNI CTCAG 3 cut(s) 24, 175, 241
BspPI GGATC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 489
BspTNI GGTCTC 1 cut(s) 746
BsrI ACTGG 1 cut(s) 77
BssMI GATC 1 cut(s) 135
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 586
Bst2BI CACGAG 1 cut(s) 586
Bst4CI ACNGT 2 cut(s) 503, 673
Bst6I CTCTTC 3 cut(s) 463, 720, 738
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 790
BstBAI YACGTR 1 cut(s) 664
BstBI TTCGAA 1 cut(s) 489
BstC8I GCNNGC 3 cut(s) 237, 524, 533
BstDEI CTNAG 3 cut(s) 11, 183, 228
BstF5I GGATG 5 cut(s) 54, 58, 383, 654, 808
BstHHI GCGC 2 cut(s) 309, 330
BstKTI GATC 1 cut(s) 138
BstMAI GTCTC 3 cut(s) 51, 129, 746
BstMBI GATC 1 cut(s) 135
BstMWI GCNNNNNNNGC 8 cut(s) 16, 28, 184, 245, 480, 528, 532, 790
BstSCI CCNGG 1 cut(s) 35
BstSFI CTRYAG 2 cut(s) 372, 438
BstV1I GCAGC 4 cut(s) 31, 244, 321, 539
BsuRI GGCC 3 cut(s) 420, 483, 568
BtsCI GGATG 5 cut(s) 54, 58, 383, 654, 808
BtsIMutI CAGTG 2 cut(s) 180, 569
Cac8I GCNNGC 3 cut(s) 237, 524, 533
CaiI CAGNNNCTG 1 cut(s) 790
CfoI GCGC 2 cut(s) 309, 330
Cfr13I GGNCC 2 cut(s) 418, 481
CseI GACGC 2 cut(s) 68, 558
Csp6I GTAC 1 cut(s) 669
CspCI CAANNNNNGTGG 2 cut(s) 376, 411
CviAII CATG 5 cut(s) 462, 475, 545, 623, 817
CviQI GTAC 1 cut(s) 669
DdeI CTNAG 3 cut(s) 11, 183, 228
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
EaeI YGGCCR 1 cut(s) 566
Eam1104I CTCTTC 3 cut(s) 463, 720, 738
EarI CTCTTC 3 cut(s) 463, 720, 738
Ecl136II GAGCTC 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 746
Eco32I GATATC 1 cut(s) 678
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 545
Eco72I CACGTG 1 cut(s) 664
Eco88I CYCGRG 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 481
EcoRI GAATTC 1 cut(s) 889
EcoRV GATATC 1 cut(s) 678
EcoT38I GRGCYC 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 5 cut(s) 465, 478, 548, 626, 820
FaiI YATR 8 cut(s) 66, 99, 351, 463, 476, 546, 624, 818
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 1 cut(s) 576
FatI CATG 5 cut(s) 461, 474, 544, 622, 816
Fnu4HI GCNGC 4 cut(s) 20, 233, 310, 553
FokI GGATG 5 cut(s) 61, 65, 370, 661, 815
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 20, 233, 310, 553
FspBI CTAG 1 cut(s) 203
FspI TGCGCA 2 cut(s) 308, 329
GlaI GCGC 2 cut(s) 308, 329
GluI GCNGC 4 cut(s) 20, 233, 310, 553
HaeIII GGCC 3 cut(s) 420, 483, 568
HapII CCGG 2 cut(s) 36, 852
HgaI GACGC 2 cut(s) 68, 558
HhaI GCGC 2 cut(s) 309, 330
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 5 cut(s) 465, 478, 548, 626, 820
Hin6I GCGC 2 cut(s) 307, 328
HinP1I GCGC 2 cut(s) 307, 328
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 5 cut(s) 104, 122, 380, 491, 787
HpaII CCGG 2 cut(s) 36, 852
HphI GGTGA 1 cut(s) 664
Hpy166II GTNNAC 1 cut(s) 838
Hpy188I TCNGA 6 cut(s) 14, 496, 574, 648, 687, 923
Hpy188III TCNNGA 4 cut(s) 115, 203, 432, 894
Hpy8I GTNNAC 1 cut(s) 838
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 891
HpyCH4III ACNGT 2 cut(s) 503, 673
HpyCH4IV ACGT 2 cut(s) 72, 663
HpyCH4V TGCA 5 cut(s) 19, 83, 248, 356, 474
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 28, 184, 245, 480, 528, 532, 790
HpyF3I CTNAG 3 cut(s) 11, 183, 228
HpySE526I ACGT 2 cut(s) 72, 663
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 5 cut(s) 465, 478, 548, 626, 820
HspAI GCGC 2 cut(s) 307, 328
Kzo9I GATC 1 cut(s) 135
LmnI GCTCC 3 cut(s) 272, 361, 434
Lsp1109I GCAGC 4 cut(s) 31, 244, 321, 539
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 72, 663
MaeIII GTNAC 1 cut(s) 91
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 5 cut(s) 113, 184, 450, 707, 725
MfeI CAATTG 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 366, 508
MlsI TGGCCA 1 cut(s) 568
MluCI AATT 7 cut(s) 251, 617, 635, 698, 830, 870, 889
MluNI TGGCCA 1 cut(s) 568
MlyI GAGTC 2 cut(s) 131, 796
MmeI TCCRAC 1 cut(s) 26
Mox20I TGGCCA 1 cut(s) 568
MscI TGGCCA 1 cut(s) 568
MseI TTAA 1 cut(s) 873
Msp20I TGGCCA 1 cut(s) 568
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 2 cut(s) 36, 852
MspR9I CCNGG 1 cut(s) 37
MunI CAATTG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 907
MwoI GCNNNNNNNGC 8 cut(s) 16, 28, 184, 245, 480, 528, 532, 790
NciI CCSGG 1 cut(s) 37
NdeII GATC 1 cut(s) 135
NlaIII CATG 5 cut(s) 465, 478, 548, 626, 820
NsbI TGCGCA 2 cut(s) 308, 329
NspV TTCGAA 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 907
PfeI GAWTC 3 cut(s) 104, 380, 491
PflFI GACNNNGTC 1 cut(s) 121
PkrI GCNGC 4 cut(s) 21, 234, 311, 554
PleI GAGTC 2 cut(s) 130, 795
PmaCI CACGTG 1 cut(s) 664
PmlI CACGTG 1 cut(s) 664
PpsI GAGTC 2 cut(s) 130, 795
Ppu21I YACGTR 1 cut(s) 664
Psp124BI GAGCTC 1 cut(s) 366
PspCI CACGTG 1 cut(s) 664
PspPI GGNCC 2 cut(s) 418, 481
PstNI CAGNNNCTG 1 cut(s) 790
PsyI GACNNNGTC 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 670
RsaNI GTAC 1 cut(s) 669
SacI GAGCTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 4 cut(s) 20, 233, 310, 553
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 2 cut(s) 418, 481
SchI GAGTC 2 cut(s) 131, 796
ScrFI CCNGG 1 cut(s) 37
SduI GDGCHC 2 cut(s) 366, 508
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 2 cut(s) 372, 438
SfuI TTCGAA 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 7 cut(s) 251, 617, 635, 698, 830, 870, 889
SsiI CCGC 1 cut(s) 322
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 35
TaaI ACNGT 2 cut(s) 503, 673
TaiI ACGT 2 cut(s) 75, 666
TaqI TCGA 2 cut(s) 489, 614
TasI AATT 7 cut(s) 251, 617, 635, 698, 830, 870, 889
TfiI GAWTC 3 cut(s) 104, 380, 491
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 2 cut(s) 187, 569
TseI GCWGC 4 cut(s) 19, 232, 309, 552
TspDTI ATGAA 4 cut(s) 114, 450, 611, 819
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 2 cut(s) 187, 569
Tth111I GACNNNGTC 1 cut(s) 121
XapI RAATTY 2 cut(s) 617, 889
XbaI TCTAGA 1 cut(s) 202
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.