RchiOBHm_Chr5g0033621

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003383
Physical Location & Seq
Forward (+)
27508218 .. 27514508
6291 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1686 bp
ATGACTGGAGGGTCATTAGGTCTACGTACTGGAAGCTATGGATCGCTACAACTACAGCAGATACAGAATGGTTTTCCACCTATTCTGCAGACAACCCCTCTTCCTCTTGCGCGAAAGCCTTCCAAGGTGCTTCTTGCAAGTAGGGAGAAGGAAAGGGTTCTGCCCTTTGTCTGTAGATTTTTGGGCCGAAAAAAGGTCATAATGCTTCTTTTGGTTGTCCTTGCTCTTTTGGTTTTCGTATCCAATTCCTTCAATGTAAATAAAGGAATGGAAGATAAACATGACATTAAAATTACTTCTACACAGAATACCATGAAAGGAAAAGATGCAAATAGAGCCCTACTTACTCCTCCTCCCCATCGTCATACTGAGTCATTTACTGCTGTTCATGGAAATTCCCCTCCATTGGCCCATCAATGTGAAAATTTTGCATTTCCTCCTCCTCCTCCTTCATCTGGTCGAAAACGAAGTGGACCTCGCCCGTGTCCAGTATGTTATCTCCCTGTGGAGCAGGCTATAGCTAGTATGCCAAGTTCTTCTTCAGAATCACCTGTACTACATAATTTGACATATGTTTATGATGAAAATCCCACTAGAACTGAACGACATGGAGGTTCTGAATTTGGTGGATTTCCAACTCTGAGACAGAGAAATGATTCATATGATATAAAAGAATCCATGACGGTCCACTGTGGATTTGTGAAAGGCAATAAACCTGGTGACCAGTCTGGATTTGATGTTGATGAAGCTGACCTTATGGAGTTGGAGACGTCCCATGAGATAATTGTTGCATCAGCTGTCTTTGGAAACTATGACATAATACAGCAGCCAACAAACATTAGTGGGTTTTCGAGGAAAAATGTTCCATTCTACATGTTTATTGATGAAGAGACTGAAGCTTATATGAAAAATTCCAGTGTCCTGGGAAACAGTAGGAAAGTAGGATTATGGAGAGTTATTGTTGTCCGTAATGTTCCTTACACTGATGCAAGACGCAATGGAAAGATTCCAAAGCTTCTATTGCACAGGCTTTTCCCCAATGTCAGATATTCTATATGGATTGATGGAAAGCTTCAGCTTGTTGTGGATCCTTATCAGGTCCTTGAGAGGTTCTTGTGGCGTCAGAATGCAAATTTTGCAATCTCAAAGCACTATAGACGGTATGATGTGTTTGAAGAGGCTGAAGCTAACAGAGCTGCTGGGAAGTACGATAATTCCTCCATTAATGAGCAGGTTGACTTCTATATCAAGGAGGGTTTACAACCTTATTCTGAGGCTAAGCTTCCAATAACGAGTGATGTTCCTGAAGGTTGTGTGATCATAAAGGAACACATTCCCATTACAAATTTATTCACCTGTAATTGGTTCAATGAAGTTGATCGTTTTACCTCCAGGGATCAGTTAAGCTTTTCCACAGTGAGGGATAAAATCATGGGAAAAGTTGATTGGAGCATTAATATGTTTTTGGATTGTGAAAGGCGCAACTTTGTAATTCAGGCATACCACAGAGAGTTGCTGGAGCAGATGCCTCCTCCCCGTCCTCCGAGGGTTGTGATTCGTCGTACACCAGCTTTGCCTCATGTTATCATATCTGTAAAGACTCCACCTGGGAAGAAGCGTGGACGAGGGGATAAGAAGCGCCATCGGAAGGTTGTTGCTGTTAGTAGAGACAACATACAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

561

Amino Acids

63.75

Weight (kDa)

9.49

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 189 - 502 2.3e-147 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AatII GACGTC 1 cut(s) 773
Acc36I ACCTGC 1 cut(s) 1222
AccI GTMKAC 1 cut(s) 22
AccII CGCG 1 cut(s) 112
AclWI GGATC 4 cut(s) 49, 1082, 1095, 1404
AcsI RAATTY 6 cut(s) 394, 424, 620, 910, 1132, 1345
AcuI CTGAAG 5 cut(s) 525, 915, 1058, 1203, 1326
AcyI GRCGYC 2 cut(s) 770, 1120
AfaI GTAC 4 cut(s) 28, 555, 1208, 1564
AfiI CCNNNNNNNGG 6 cut(s) 193, 406, 455, 1362, 1419, 1648
AflIII ACRYGT 1 cut(s) 873
AgsI TTSAA 3 cut(s) 253, 1175, 1369
AjnI CCWGG 4 cut(s) 715, 921, 1391, 1606
AjuI GAANNNNNNNTTGG 4 cut(s) 523, 555, 1429, 1461
Alw26I GTCTC 4 cut(s) 637, 761, 884, 1662
AlwI GGATC 4 cut(s) 49, 1082, 1095, 1404
AoxI GGCC 2 cut(s) 184, 408
ApeKI GCWGC 2 cut(s) 826, 1196
ApoI RAATTY 6 cut(s) 394, 424, 620, 910, 1132, 1345
AseI ATTAAT 2 cut(s) 1224, 1455
Asp700I GAANNNNTTC 4 cut(s) 118, 156, 655, 1332
AspLEI GCGC 3 cut(s) 112, 1482, 1641
AspS9I GGNCC 5 cut(s) 184, 409, 473, 685, 1099
AsuHPI GGTGA 3 cut(s) 540, 731, 1345
AvaII GGWCC 3 cut(s) 473, 685, 1099
BamHI GGATCC 1 cut(s) 1087
BanII GRGCYC 1 cut(s) 340
BbvI GCAGC 2 cut(s) 838, 1183
BccI CCATC 4 cut(s) 366, 420, 1058, 1650
BciT130I CCWGG 4 cut(s) 717, 923, 1393, 1608
BciVI GTATCC 1 cut(s) 250
BclI TGATCA 1 cut(s) 1317
BcoDI GTCTC 4 cut(s) 637, 761, 884, 1662
BfaI CTAG 2 cut(s) 522, 594
BfmI CTRYAG 5 cut(s) 53, 86, 172, 516, 1153
BfoI RGCGCY 1 cut(s) 1642
BfuAI ACCTGC 1 cut(s) 1222
BfuI GTATCC 1 cut(s) 250
BisI GCNGC 2 cut(s) 827, 1197
BlpI GCTNAGC 1 cut(s) 1278
BlsI GCNGC 2 cut(s) 828, 1198
Bme1390I CCNGG 4 cut(s) 717, 923, 1393, 1608
Bme18I GGWCC 3 cut(s) 473, 685, 1099
BmgT120I GGNCC 5 cut(s) 184, 409, 473, 685, 1099
BmiI GGNNCC 1 cut(s) 1089
BmrFI CCNGG 4 cut(s) 717, 923, 1393, 1608
BmsI GCATC 4 cut(s) 316, 800, 976, 1515
BpmI CTGGAG 3 cut(s) 27, 1375, 1538
Bpu1102I GCTNAGC 1 cut(s) 1278
BpuEI CTTGAG 1 cut(s) 1124
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 2 cut(s) 585, 1092
BsaHI GRCGYC 2 cut(s) 770, 1120
BsaJI CCNNGG 5 cut(s) 123, 922, 1392, 1544, 1607
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 6 cut(s) 193, 406, 455, 1362, 1419, 1648
Bse1I ACTGG 5 cut(s) 10, 34, 488, 724, 915
Bse3DI GCAATG 1 cut(s) 1003
Bse8I GATNNNNATC 2 cut(s) 585, 1092
BseBI CCWGG 4 cut(s) 717, 923, 1393, 1608
BseDI CCNNGG 5 cut(s) 123, 922, 1392, 1544, 1607
BseJI GATNNNNATC 2 cut(s) 585, 1092
BseLI CCNNNNNNNGG 6 cut(s) 193, 406, 455, 1362, 1419, 1648
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 3 cut(s) 360, 632, 1263
BseNI ACTGG 5 cut(s) 10, 34, 488, 724, 915
BseRI GAGGAG 6 cut(s) 339, 342, 429, 432, 435, 1521
BseXI GCAGC 2 cut(s) 838, 1183
BseYI CCCAGC 1 cut(s) 1199
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 186, 410
BslFI GGGAC 1 cut(s) 757
BslI CCNNNNNNNGG 6 cut(s) 193, 406, 455, 1362, 1419, 1648
BsmAI GTCTC 4 cut(s) 637, 761, 884, 1662
BsmBI CGTCTC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 757
BsmI GAATGC 1 cut(s) 1132
BsnI GGCC 2 cut(s) 186, 410
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
Bsp1720I GCTNAGC 1 cut(s) 1278
BspANI GGCC 2 cut(s) 186, 410
BspCNI CTCAG 3 cut(s) 361, 633, 1264
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 1089
BspMAI CTGCAG 1 cut(s) 90
BspMI ACCTGC 1 cut(s) 1222
BspPI GGATC 4 cut(s) 49, 1082, 1095, 1404
BsrDI GCAATG 1 cut(s) 1003
BsrI ACTGG 5 cut(s) 10, 34, 488, 724, 915
BssECI CCNNGG 5 cut(s) 123, 922, 1392, 1544, 1607
BssMI GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
BssNI GRCGYC 2 cut(s) 770, 1120
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 4 cut(s) 717, 923, 1393, 1608
Bst4CI ACNGT 5 cut(s) 685, 692, 932, 1161, 1417
Bst6I CTCTTC 3 cut(s) 105, 882, 1170
BstACI GRCGYC 2 cut(s) 770, 1120
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 4 cut(s) 369, 641, 1272, 1278
BstEII GGTNACC 1 cut(s) 719
BstFNI CGCG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 1642
BstHHI GCGC 3 cut(s) 112, 1482, 1641
BstKTI GATC 5 cut(s) 44, 1090, 1320, 1381, 1399
BstMAI GTCTC 4 cut(s) 637, 761, 884, 1662
BstMBI GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
BstMWI GCNNNNNNNGC 4 cut(s) 335, 1021, 1136, 1193
BstNI CCWGG 4 cut(s) 717, 923, 1393, 1608
BstNSI RCATGY 1 cut(s) 877
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 4 cut(s) 715, 921, 1391, 1606
BstSFI CTRYAG 5 cut(s) 53, 86, 172, 516, 1153
BstSNI TACGTA 1 cut(s) 26
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 838, 1183
BstX2I RGATCY 1 cut(s) 1087
BstXI CCANNNNNNTGG 1 cut(s) 922
BstYI RGATCY 1 cut(s) 1087
BsuI GTATCC 1 cut(s) 250
BsuRI GGCC 2 cut(s) 186, 410
BtsIMutI CAGTG 4 cut(s) 688, 922, 981, 1422
BveI ACCTGC 1 cut(s) 1222
Cac8I GCNNGC 1 cut(s) 513
CfoI GCGC 3 cut(s) 112, 1482, 1641
Cfr13I GGNCC 5 cut(s) 184, 409, 473, 685, 1099
CseI GACGC 2 cut(s) 1002, 1109
CsiI ACCWGGT 1 cut(s) 715
Csp6I GTAC 4 cut(s) 27, 554, 1207, 1563
CviAII CATG 9 cut(s) 281, 313, 389, 608, 679, 776, 874, 1432, 1580
CviQI GTAC 4 cut(s) 27, 554, 1207, 1563
DdeI CTNAG 4 cut(s) 369, 641, 1272, 1278
DpnI GATC 5 cut(s) 43, 1089, 1319, 1380, 1398
DpnII GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 3 cut(s) 105, 882, 1170
EarI CTCTTC 3 cut(s) 105, 882, 1170
Eco105I TACGTA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 340
Eco47I GGWCC 3 cut(s) 473, 685, 1099
Eco57I CTGAAG 5 cut(s) 525, 915, 1058, 1203, 1326
Eco91I GGTNACC 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 1099
EcoO65I GGTNACC 1 cut(s) 719
EcoRII CCWGG 4 cut(s) 715, 921, 1391, 1606
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 761
FaeI CATG 9 cut(s) 284, 316, 392, 611, 682, 779, 877, 1435, 1583
FalI AAGNNNNNCTT 4 cut(s) 523, 555, 738, 770
FaqI GGGAC 1 cut(s) 757
FatI CATG 9 cut(s) 280, 312, 388, 607, 678, 775, 873, 1431, 1579
FauNDI CATATG 2 cut(s) 571, 661
FbaI TGATCA 1 cut(s) 1317
FblI GTMKAC 1 cut(s) 22
Fnu4HI GCNGC 2 cut(s) 827, 1197
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 2 cut(s) 827, 1197
FspBI CTAG 2 cut(s) 522, 594
GlaI GCGC 3 cut(s) 111, 1481, 1640
GluI GCNGC 2 cut(s) 827, 1197
GsaI CCCAGC 1 cut(s) 1203
GsuI CTGGAG 3 cut(s) 27, 1375, 1538
HaeII RGCGCY 1 cut(s) 1642
HaeIII GGCC 2 cut(s) 186, 410
HgaI GACGC 2 cut(s) 1002, 1109
HhaI GCGC 3 cut(s) 112, 1482, 1641
Hin1I GRCGYC 2 cut(s) 770, 1120
Hin1II CATG 9 cut(s) 284, 316, 392, 611, 682, 779, 877, 1435, 1583
Hin6I GCGC 3 cut(s) 110, 1480, 1639
HinP1I GCGC 3 cut(s) 110, 1480, 1639
HincII GTYRAC 1 cut(s) 1237
HindII GTYRAC 1 cut(s) 1237
HindIII AAGCTT 5 cut(s) 897, 1013, 1070, 1280, 1405
HinfI GANTC 7 cut(s) 371, 545, 656, 674, 1006, 1555, 1600
HphI GGTGA 3 cut(s) 540, 731, 1345
Hpy166II GTNNAC 7 cut(s) 23, 473, 688, 1237, 1259, 1565, 1622
Hpy188I TCNGA 8 cut(s) 544, 619, 642, 1046, 1125, 1273, 1545, 1647
Hpy188III TCNNGA 2 cut(s) 729, 1304
Hpy8I GTNNAC 7 cut(s) 23, 473, 688, 1237, 1259, 1565, 1622
Hpy99I CGWCG 1 cut(s) 1563
HpyAV CCTTC 6 cut(s) 129, 142, 259, 459, 1301, 1642
HpyCH4III ACNGT 5 cut(s) 685, 692, 932, 1161, 1417
HpyCH4IV ACGT 2 cut(s) 25, 770
HpyCH4V TGCA 9 cut(s) 88, 137, 329, 431, 791, 989, 1024, 1130, 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 1021, 1136, 1193
HpyF3I CTNAG 4 cut(s) 369, 641, 1272, 1278
HpySE526I ACGT 2 cut(s) 25, 770
Hsp92I GRCGYC 2 cut(s) 770, 1120
Hsp92II CATG 9 cut(s) 284, 316, 392, 611, 682, 779, 877, 1435, 1583
HspAI GCGC 3 cut(s) 110, 1480, 1639
Ksp22I TGATCA 1 cut(s) 1317
Kzo9I GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
LmnI GCTCC 3 cut(s) 508, 1449, 1519
Lsp1109I GCAGC 2 cut(s) 838, 1183
LweI GCATC 4 cut(s) 316, 800, 976, 1515
MabI ACCWGGT 1 cut(s) 715
MaeI CTAG 2 cut(s) 522, 594
MaeII ACGT 2 cut(s) 25, 770
MaeIII GTNAC 1 cut(s) 719
MalI GATC 5 cut(s) 43, 1089, 1319, 1380, 1398
MboI GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
MboII GAAGA 7 cut(s) 92, 284, 528, 531, 899, 1187, 1624
MflI RGATCY 1 cut(s) 1087
MhlI GDGCHC 1 cut(s) 340
MlyI GAGTC 2 cut(s) 380, 1594
MmeI TCCRAC 2 cut(s) 659, 744
MroXI GAANNNNTTC 4 cut(s) 118, 156, 655, 1332
MseI TTAA 4 cut(s) 288, 1224, 1403, 1455
MslI CAYNNNNRTG 2 cut(s) 417, 1457
MspA1I CMGCKG 1 cut(s) 797
MspR9I CCNGG 4 cut(s) 717, 923, 1393, 1608
Mva1269I GAATGC 1 cut(s) 1132
MvaI CCWGG 4 cut(s) 717, 923, 1393, 1608
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 4 cut(s) 335, 1021, 1136, 1193
NdeI CATATG 2 cut(s) 571, 661
NdeII GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
NlaIII CATG 9 cut(s) 284, 316, 392, 611, 682, 779, 877, 1435, 1583
NlaIV GGNNCC 1 cut(s) 1089
NmuCI GTSAC 1 cut(s) 719
NspI RCATGY 1 cut(s) 877
PciI ACATGT 1 cut(s) 873
PctI GAATGC 1 cut(s) 1132
PdmI GAANNNNTTC 4 cut(s) 118, 156, 655, 1332
PfeI GAWTC 5 cut(s) 545, 656, 674, 1006, 1555
PkrI GCNGC 2 cut(s) 828, 1198
PleI GAGTC 2 cut(s) 379, 1594
PpsI GAGTC 2 cut(s) 379, 1594
Ppu21I YACGTR 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 1099
PscI ACATGT 1 cut(s) 873
PshBI ATTAAT 2 cut(s) 1224, 1455
Psp5II RGGWCCY 1 cut(s) 1099
Psp6I CCWGG 4 cut(s) 715, 921, 1391, 1606
PspEI GGTNACC 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 1199
PspGI CCWGG 4 cut(s) 715, 921, 1391, 1606
PspN4I GGNNCC 1 cut(s) 1089
PspPI GGNCC 5 cut(s) 184, 409, 473, 685, 1099
PspPPI RGGWCCY 1 cut(s) 1099
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
PstI CTGCAG 1 cut(s) 90
PsuI RGATCY 1 cut(s) 1087
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 4 cut(s) 28, 555, 1208, 1564
RsaNI GTAC 4 cut(s) 27, 554, 1207, 1563
RseI CAYNNNNRTG 2 cut(s) 417, 1457
SaqAI TTAA 4 cut(s) 288, 1224, 1403, 1455
SatI GCNGC 2 cut(s) 827, 1197
Sau3AI GATC 5 cut(s) 41, 1087, 1317, 1378, 1396
Sau96I GGNCC 5 cut(s) 184, 409, 473, 685, 1099
SchI GAGTC 2 cut(s) 380, 1594
ScrFI CCNGG 4 cut(s) 717, 923, 1393, 1608
SduI GDGCHC 1 cut(s) 340
SexAI ACCWGGT 1 cut(s) 715
SfaNI GCATC 4 cut(s) 316, 800, 976, 1515
SfcI CTRYAG 5 cut(s) 53, 86, 172, 516, 1153
SinI GGWCC 3 cut(s) 473, 685, 1099
SmiMI CAYNNNNRTG 2 cut(s) 417, 1457
SmlI CTYRAG 1 cut(s) 1103
SmoI CTYRAG 1 cut(s) 1103
SnaBI TACGTA 1 cut(s) 26
SspMI CTAG 2 cut(s) 522, 594
StyD4I CCNGG 4 cut(s) 715, 921, 1391, 1606
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 5 cut(s) 685, 692, 932, 1161, 1417
TaiI ACGT 2 cut(s) 28, 773
TaqI TCGA 2 cut(s) 460, 851
TatI WGTACW 1 cut(s) 553
TfiI GAWTC 5 cut(s) 545, 656, 674, 1006, 1555
Tru1I TTAA 4 cut(s) 288, 1224, 1403, 1455
Tru9I TTAA 4 cut(s) 288, 1224, 1403, 1455
TscAI CASTG 4 cut(s) 695, 922, 988, 1422
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 2 cut(s) 826, 1196
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 9 cut(s) 329, 377, 441, 597, 648, 759, 900, 920, 1386
TspGWI ACGGA 1 cut(s) 956
TspRI CASTG 4 cut(s) 695, 922, 988, 1422
VpaK11BI GGWCC 3 cut(s) 473, 685, 1099
VspI ATTAAT 2 cut(s) 1224, 1455
XapI RAATTY 6 cut(s) 394, 424, 620, 910, 1132, 1345
XceI RCATGY 1 cut(s) 877
XmiI GTMKAC 1 cut(s) 22
XmnI GAANNNNTTC 4 cut(s) 118, 156, 655, 1332
XspI CTAG 2 cut(s) 522, 594
ZraI GACGTC 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.