RchiOBHm_Chr5g0033981

Dynein light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014694
Physical Location & Seq
Forward (+)
27878552 .. 27879283
732 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGGAAAAATCCGATCAAAAACAGAAGCAGCGCCACGAGCAAAACATATTGTTACCTCAACAACAACAACATAAGCAAGGCTACTCCGATCATGTCTGTCGAGATAGAGTTCAGGATCAACCTCAGAGCAAAGTACAAGCAGGTCTAATTAGAGAAGTAGACCAGGAGGTGAAGTTAGCTGCCACCGCGATCAGCCTCAACGTACGGTTGAGATCATCGTCCCACATGCCGCACCACATGCAAGACCGTGCTCTCCGCCGCACCAGATCACTCATCGACTCAGCCGCCGCCGCAGCAAAAGCACTTTCCTCCAAACCACGGCCAAACCCAACTCACATTGCTCGAGCTCTCAAAAAGGAGTTTGACTCGTTGTACGGACCGGCGTGGCACTGCGTGGTGGGAAAGAGTTTCGGGTCGTTCGTGACTCACTCGCCGGGTGGTTTCCTCTATTTTTCTATCGACGACTCGCTGTCGGTGCTGCTCTTTAAGACGGAGGTTCAGTTGGTCACCGAACAACAACCACGGAAACCGGCGAATTGA

Protein Analysis

179

Amino Acids

20.21

Weight (kDa)

9.8

Isoelectric Point (pI)

59.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynein_light PF01221 73 - 164 1.5e-25 Dynein light chain type 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014694)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20170
malus_domestica MD03G1226300.v1.1 MD11G1244800.v1.1
prunus_persica Prupe.4G183100_v2.0.a1
pyrus_communis pycom03g17400
rosa_chinensis RchiOBHm_Chr5g0033981
rosa_laevigata RLG00000033516
rosa_multiflora Rmu_co8482145.1_g000001 Rmu_sc0012341.1_g000008 Rmu_sc0014753.1_g000004
rosa_roxburghii Rroxscaffold_1G00046250
rosa_rugosa Rorug05G0143900
rosa_samantha Rh5AG234800 Rh5BG235600 Rh5CG265400 Rh5DG242000
rosa_wichuraiana Rw5G021480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 131
AccI GTMKAC 1 cut(s) 159
AccII CGCG 1 cut(s) 188
AciI CCGC 7 cut(s) 186, 230, 256, 259, 285, 288, 291
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 320
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 3 cut(s) 135, 204, 374
AfiI CCNNNNNNNGG 1 cut(s) 318
AhdI GACNNNNNGTC 1 cut(s) 469
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 2 cut(s) 179, 347
AluI AGCT 2 cut(s) 179, 347
Alw21I GWGCWC 2 cut(s) 253, 349
AlwI GGATC 1 cut(s) 123
Ama87I CYCGRG 1 cut(s) 342
AoxI GGCC 1 cut(s) 320
ApeKI GCWGC 4 cut(s) 28, 179, 293, 478
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 377
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 2 cut(s) 181, 499
AvaI CYCGRG 1 cut(s) 342
AvaII GGWCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 349
BauI CACGAG 1 cut(s) 35
Bbv12I GWGCWC 2 cut(s) 253, 349
BbvI GCAGC 4 cut(s) 40, 166, 305, 465
BceAI ACGGC 1 cut(s) 335
BciT130I CCWGG 1 cut(s) 164
BcnI CCSGG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 34
BfuAI ACCTGC 1 cut(s) 131
BisI GCNGC 9 cut(s) 29, 180, 230, 259, 285, 288, 291, 294, 479
BlsI GCNGC 9 cut(s) 30, 181, 231, 260, 286, 289, 292, 295, 480
Bme1390I CCNGG 2 cut(s) 164, 435
Bme18I GGWCC 1 cut(s) 377
BmeRI GACNNNNNGTC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 342
BmgT120I GGNCC 1 cut(s) 377
BmrFI CCNGG 2 cut(s) 164, 435
BplI GAGNNNNNCTC 2 cut(s) 350, 382
BpuMI CCSGG 1 cut(s) 435
BsaJI CCNNGG 2 cut(s) 317, 521
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 485, 515
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse118I RCCGGY 2 cut(s) 379, 529
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 317, 521
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 137, 294
BseXI GCAGC 4 cut(s) 40, 166, 305, 465
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 1 cut(s) 322
BsiHKAI GWGCWC 2 cut(s) 253, 349
BsiHKCI CYCGRG 1 cut(s) 342
BsiSI CCGG 3 cut(s) 380, 434, 530
BsiWI CGTACG 1 cut(s) 202
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 342
Bsp1286I GDGCHC 2 cut(s) 253, 349
Bsp143I GATC 6 cut(s) 13, 88, 115, 189, 212, 266
BspACI CCGC 7 cut(s) 186, 230, 256, 259, 285, 288, 291
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 2 cut(s) 136, 293
BspFNI CGCG 1 cut(s) 188
BspMI ACCTGC 1 cut(s) 131
BspPI GGATC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 2 cut(s) 379, 529
BssAI RCCGGY 2 cut(s) 379, 529
BssECI CCNNGG 2 cut(s) 317, 521
BssMI GATC 6 cut(s) 13, 88, 115, 189, 212, 266
BssSI CACGAG 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 207, 248
BstAPI GCANNNNNTGC 1 cut(s) 238
BstDEI CTNAG 2 cut(s) 123, 280
BstDSI CCRYGG 2 cut(s) 317, 521
BstEII GGTNACC 1 cut(s) 505
BstFNI CGCG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 6 cut(s) 16, 91, 118, 192, 215, 269
BstMBI GATC 6 cut(s) 13, 88, 115, 189, 212, 266
BstMWI GCNNNNNNNGC 7 cut(s) 37, 185, 238, 290, 293, 299, 475
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 2 cut(s) 229, 241
BstPI GGTNACC 1 cut(s) 505
BstSCI CCNGG 2 cut(s) 162, 433
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 4 cut(s) 40, 166, 305, 465
BsuRI GGCC 1 cut(s) 322
BtgI CCRYGG 2 cut(s) 317, 521
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
BveI ACCTGC 1 cut(s) 131
CfoI GCGC 1 cut(s) 33
Cfr10I RCCGGY 2 cut(s) 379, 529
Cfr13I GGNCC 1 cut(s) 377
CpoI CGGWCCG 1 cut(s) 377
Csp6I GTAC 3 cut(s) 134, 203, 373
CspI CGGWCCG 1 cut(s) 377
CviAII CATG 3 cut(s) 92, 226, 238
CviJI RGCY 6 cut(s) 81, 179, 195, 284, 322, 347
CviKI_1 RGCY 6 cut(s) 81, 179, 195, 284, 322, 347
CviQI GTAC 3 cut(s) 134, 203, 373
DdeI CTNAG 2 cut(s) 123, 280
DpnI GATC 6 cut(s) 15, 90, 117, 191, 214, 268
DpnII GATC 6 cut(s) 13, 88, 115, 189, 212, 266
DraIII CACNNNGTG 1 cut(s) 394
DriI GACNNNNNGTC 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 320
Eam1105I GACNNNNNGTC 1 cut(s) 469
EciI GGCGGA 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 347
Eco88I CYCGRG 1 cut(s) 342
Eco91I GGTNACC 1 cut(s) 505
EcoICRI GAGCTC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 505
EcoRII CCWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 349
FaeI CATG 3 cut(s) 95, 229, 241
FaiI YATR 5 cut(s) 47, 72, 93, 227, 239
FaqI GGGAC 1 cut(s) 205
FatI CATG 3 cut(s) 91, 225, 237
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 9 cut(s) 29, 180, 230, 259, 285, 288, 291, 294, 479
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 9 cut(s) 29, 180, 230, 259, 285, 288, 291, 294, 479
GlaI GCGC 1 cut(s) 32
GluI GCNGC 9 cut(s) 29, 180, 230, 259, 285, 288, 291, 294, 479
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 1 cut(s) 322
HapII CCGG 3 cut(s) 380, 434, 530
HhaI GCGC 1 cut(s) 33
Hin1II CATG 3 cut(s) 95, 229, 241
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HinfI GANTC 4 cut(s) 278, 365, 424, 464
HpaII CCGG 3 cut(s) 380, 434, 530
HphI GGTGA 2 cut(s) 181, 499
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 3 cut(s) 13, 88, 126
Hpy188III TCNNGA 3 cut(s) 101, 113, 421
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 464
HpyCH4III ACNGT 2 cut(s) 207, 248
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 241
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 185, 238, 290, 293, 299, 475
HpyF3I CTNAG 2 cut(s) 123, 280
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 3 cut(s) 95, 229, 241
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 13, 88, 115, 189, 212, 266
LpnPI CCDG 7 cut(s) 98, 126, 149, 176, 277, 393, 447
Lsp1109I GCAGC 4 cut(s) 40, 166, 305, 465
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 3 cut(s) 51, 421, 505
MalI GATC 6 cut(s) 15, 90, 117, 191, 214, 268
MboI GATC 6 cut(s) 13, 88, 115, 189, 212, 266
MhlI GDGCHC 2 cut(s) 253, 349
MluCI AATT 2 cut(s) 147, 535
MlyI GAGTC 4 cut(s) 272, 359, 418, 458
MnlI CCTC 7 cut(s) 66, 132, 160, 206, 319, 455, 487
MseI TTAA 1 cut(s) 486
MspI CCGG 3 cut(s) 380, 434, 530
MspR9I CCNGG 2 cut(s) 164, 435
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 7 cut(s) 37, 185, 238, 290, 293, 299, 475
NciI CCSGG 1 cut(s) 435
NdeII GATC 6 cut(s) 13, 88, 115, 189, 212, 266
NlaIII CATG 3 cut(s) 95, 229, 241
NmuCI GTSAC 2 cut(s) 421, 505
NspI RCATGY 2 cut(s) 229, 241
PaeR7I CTCGAG 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 417
Pfl23II CGTACG 1 cut(s) 202
PkrI GCNGC 9 cut(s) 30, 181, 231, 260, 286, 289, 292, 295, 480
PleI GAGTC 4 cut(s) 272, 359, 418, 458
PpsI GAGTC 4 cut(s) 272, 359, 418, 458
Psp124BI GAGCTC 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 162
PspEI GGTNACC 1 cut(s) 505
PspGI CCWGG 1 cut(s) 162
PspLI CGTACG 1 cut(s) 202
PspPI GGNCC 1 cut(s) 377
PspXI VCTCGAGB 1 cut(s) 342
RsaI GTAC 3 cut(s) 135, 204, 374
RsaNI GTAC 3 cut(s) 134, 203, 373
Rsr2I CGGWCCG 1 cut(s) 377
RsrII CGGWCCG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 9 cut(s) 29, 180, 230, 259, 285, 288, 291, 294, 479
Sau3AI GATC 6 cut(s) 13, 88, 115, 189, 212, 266
Sau96I GGNCC 1 cut(s) 377
SchI GAGTC 4 cut(s) 272, 359, 418, 458
ScrFI CCNGG 2 cut(s) 164, 435
SduI GDGCHC 2 cut(s) 253, 349
SetI ASST 8 cut(s) 58, 124, 145, 171, 181, 204, 349, 498
Sfr274I CTCGAG 1 cut(s) 342
SinI GGWCC 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 342
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 2 cut(s) 147, 535
SsiI CCGC 7 cut(s) 186, 230, 256, 259, 285, 288, 291
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 2 cut(s) 162, 433
TaaI ACNGT 2 cut(s) 207, 248
TaiI ACGT 1 cut(s) 204
TaqI TCGA 4 cut(s) 100, 276, 343, 459
TasI AATT 2 cut(s) 147, 535
TatI WGTACW 1 cut(s) 133
TauI GCSGC 5 cut(s) 232, 261, 287, 290, 293
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TscAI CASTG 1 cut(s) 395
TseFI GTSAC 2 cut(s) 421, 505
TseI GCWGC 4 cut(s) 28, 179, 293, 478
Tsp45I GTSAC 2 cut(s) 421, 505
TspGWI ACGGA 3 cut(s) 390, 506, 538
TspRI CASTG 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 377
XceI RCATGY 2 cut(s) 229, 241
XhoI CTCGAG 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.