RchiOBHm_Chr5g0037891

zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016791
Physical Location & Seq
Forward (+)
32532286 .. 32536036
3751 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1821 bp
ATGCAGATGGACAGCAGTATTTCACAAATTGAGGTAGACATGAGGATACCTCAACATGCAACACCATCTCAGCAAGTCTCATTGGAGAATTTGGGGTCAAATGTCCAGATTGCAAGCAATATTTCCAGAGCCAGTGATCTACTCATGGCAGTAACTTCAAGTGCTGAAATCAACAGAATGAAAATAAGTGAGTTAGCTTCGGAAGCAATGGAAGAGCTAACTAAATTGGCCCTAGCTCGAGAACCTCTATGGCAGTGTGACATAGAGAGCAATACTGAATTTCTTAGTGATATCGAGTATCTGAGGGAATTTGGACACATAAGTACGTCATTGATAGAAATCATTAGAATGGTTGAGGTGGGAGACTCTCAATCTGACCTGCCAAGATTCGGAAGCATTAACAATTCTGAATTTTCGATGGAAAGTGAATATAGGCCAATATCGACAACAAATCAACCTGGAAAACCAGTTAGTTCGGAATCTTCACGAGCTATTGACTATGTCAAAATGAAAGCAGTCAACATTGTTCAATTGCTAATGGATTTGCAATGGCTACTGGTGTTTCCTAATATTGTATCTAGAGCAAAAGTAGTGGGAGTATTATCATCAACAAGATTCGAAGGCAACTATGATGGAACTCTGCAAGTGATGACAGCTGAGTTTCATGCCCCAACACCACTTATTCCCGCTAGAGAAAGTTATTTTGCAAGGTATAGTAAACAGCTGGACAGCGGAATGTGGGTAGTTGTTGATGTATCCTTGGAAAAATTGTTTCAGCTTCCATCAACAAACTTTCGAAGACAACCATCAGGATGTTTAATTCAGGAAATGTCAAATGGATGCTCTAAGGTCATATGGGTGGAGCATGTGGAAGTGGATAATAGAATGGTTCACAATATGTTTAAGCCACTGGTCAGTTCTGGTTTTGCATTTAGCGCAAAGCGTTGGGTTTCCACTTTAGTTCGACAATGTGAATGGTTGGCAACTTTAAATTCTACAAGCCCTCCTACATCAGATTGTATGTTCGTATCGCGAGCTGGGAGAAAAAGCTTGTTGAAGCTTTCGGAGAGGATGATGAGAAGTTTTGTTGCTGATATTAGTGCTTCTACAGAAAACAAATGGAGGGCACTACCTGTATTTGGTGCCGAAGATGTAAAGGTCACAACTAAAACTAGCTTAAATGACCCTGGAAAGCCTCCAGGTACCACCTTATTATTTGCTACTTCTGTCCATCTTCCAGTCCCAAGAACTCAAATATACAATCTGCTTCGCAATGGTGAATCTCGAATCATGTGGGATATGATGTCGTATGAAAAAGATACAAGAGAAATTGCTTATATCGCAACTGGGGACCCTCCAGGAAATCGCGTTTCGATAATTGAAGTAGAAAATGCAGGAAAGGGCAGCGAGAGTGGTACTTCACAATCCTCACTTTCCCTTCCTCTAACTTTGCAGGGTGTAACGAAGGAGATTGAAGTTTTCTACTTCCAGGAGAGTTACACAGACTCAAATGGCTCGTACATAGTTTACGCACCGATTGACCAGTTCGCCATGTCTGCTCTCTTGGATGGCTCAAACCCAGACCATGTTGCTATTCTCGCATCCGGGTTTTCCATCCTTCCTGATAGACCAATGGCCAATGATGAATCTGGTGGAAGTCTGTTGACTCTTGCGTTGCACATCACCGAAGGAGGAGCAGCGGAGGAATATATCCCTCCTCAAACTTTTCAAAATATACTCAACATTATTGCTGGAACTGTGATTCAAATCACAGAAGTATTATCAAATGAGGGAGAAAATAGTGTTGAATCAGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

606

Amino Acids

67.06

Weight (kDa)

4.86

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 141 - 325 2.3e-32 START domain
PDF2_C PF25797 345 - 465 1.2e-33 PDF2-like, C-terminal domain
PDF2_C PF25797 487 - 594 7e-20 PDF2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016791)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g22020 FvH4_3g22021
malus_domestica MD11G1223900.v1.1
prunus_persica Prupe.4G205900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037891
rosa_laevigata RLG00000033820
rosa_multiflora Rmu_sc0005416.1_g000025
rosa_roxburghii Rroxscaffold_1G00043030
rosa_rugosa Rorug05G0168500
rosa_samantha Rh5AG257100 Rh5BG259700 Rh5CG292700 Rh5DG269600
rosa_wichuraiana Rw5G023850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 387
Acc65I GGTACC 1 cut(s) 1202
AccB1I GGYRCC 2 cut(s) 1142, 1202
AccI GTMKAC 1 cut(s) 36
AccII CGCG 2 cut(s) 1033, 1368
AciI CCGC 3 cut(s) 687, 732, 1700
AcoI YGGCCR 1 cut(s) 1635
AcsI RAATTY 5 cut(s) 88, 278, 308, 410, 991
AfaI GTAC 4 cut(s) 325, 1204, 1417, 1520
AfiI CCNNNNNNNGG 2 cut(s) 389, 1139
AgsI TTSAA 8 cut(s) 159, 530, 1057, 1382, 1475, 1730, 1766, 1808
AjnI CCWGG 5 cut(s) 457, 1186, 1198, 1357, 1488
Alw26I GTCTC 2 cut(s) 82, 357
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 3 cut(s) 228, 434, 1635
ApeKI GCWGC 2 cut(s) 1404, 1697
ApoI RAATTY 5 cut(s) 88, 278, 308, 410, 991
Asp718I GGTACC 1 cut(s) 1202
AspLEI GCGC 1 cut(s) 938
AspS9I GGNCC 2 cut(s) 229, 1351
AsuC2I CCSGG 1 cut(s) 1606
AsuHPI GGTGA 2 cut(s) 1289, 1675
AsuII TTCGAA 2 cut(s) 618, 796
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 1351
BaeGI GKGCMC 1 cut(s) 1129
BalI TGGCCA 1 cut(s) 1637
BanI GGYRCC 2 cut(s) 1142, 1202
BauI CACGAG 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 805
BbvI GCAGC 2 cut(s) 1416, 1709
BccI CCATC 8 cut(s) 73, 412, 626, 790, 814, 1239, 1562, 1622
BciT130I CCWGG 5 cut(s) 459, 1188, 1200, 1359, 1490
BciVI GTATCC 2 cut(s) 39, 766
BcnI CCSGG 1 cut(s) 1606
BcoDI GTCTC 2 cut(s) 82, 357
BfaI CTAG 4 cut(s) 233, 579, 690, 1173
BfmI CTRYAG 1 cut(s) 1107
BfuAI ACCTGC 1 cut(s) 387
BfuI GTATCC 2 cut(s) 39, 766
BisI GCNGC 2 cut(s) 1405, 1698
BlsI GCNGC 2 cut(s) 1406, 1699
Bme1390I CCNGG 6 cut(s) 459, 1188, 1200, 1359, 1490, 1606
Bme18I GGWCC 1 cut(s) 1351
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 2 cut(s) 229, 1351
BmiI GGNNCC 4 cut(s) 1144, 1204, 1352, 1353
BmrFI CCNGG 6 cut(s) 459, 1188, 1200, 1359, 1490, 1606
BmrI ACTGGG 1 cut(s) 1356
BmsI GCATC 2 cut(s) 830, 1610
BmuI ACTGGG 1 cut(s) 1356
BpiI GAAGAC 1 cut(s) 805
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpmI CTGGAG 2 cut(s) 1182, 1341
Bpu14I TTCGAA 2 cut(s) 618, 796
BpuMI CCSGG 1 cut(s) 1606
BsaBI GATNNNNATC 2 cut(s) 338, 1766
BsaJI CCNNGG 2 cut(s) 759, 1186
BsaXI ACNNNNNCTCC 2 cut(s) 988, 1018
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 1139
Bse1I ACTGG 7 cut(s) 132, 467, 561, 915, 1238, 1351, 1543
Bse3DI GCAATG 3 cut(s) 213, 554, 1279
Bse8I GATNNNNATC 2 cut(s) 338, 1766
BseBI CCWGG 5 cut(s) 459, 1188, 1200, 1359, 1490
BseDI CCNNGG 2 cut(s) 759, 1186
BseGI GGATG 6 cut(s) 818, 845, 1077, 1573, 1601, 1614
BseJI GATNNNNATC 2 cut(s) 338, 1766
BseLI CCNNNNNNNGG 2 cut(s) 389, 1139
BseMI GCAATG 3 cut(s) 213, 554, 1279
BseMII CTCAG 3 cut(s) 83, 293, 648
BseNI ACTGG 7 cut(s) 132, 467, 561, 915, 1238, 1351, 1543
BseRI GAGGAG 2 cut(s) 1707, 1707
BseSI GKGCMC 1 cut(s) 1129
BseXI GCAGC 2 cut(s) 1416, 1709
BseYI CCCAGC 1 cut(s) 1037
Bsh1236I CGCG 2 cut(s) 1033, 1368
BshFI GGCC 3 cut(s) 230, 436, 1637
BshNI GGYRCC 2 cut(s) 1142, 1202
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 1605
BslFI GGGAC 2 cut(s) 1226, 1364
BslI CCNNNNNNNGG 2 cut(s) 389, 1139
BsmAI GTCTC 2 cut(s) 82, 357
BsmFI GGGAC 2 cut(s) 1226, 1364
BsnI GGCC 3 cut(s) 230, 436, 1637
BsoBI CYCGRG 1 cut(s) 237
Bsp119I TTCGAA 2 cut(s) 618, 796
Bsp1286I GDGCHC 1 cut(s) 1129
Bsp143I GATC 1 cut(s) 136
Bsp68I TCGCGA 1 cut(s) 1033
BspACI CCGC 3 cut(s) 687, 732, 1700
BspANI GGCC 3 cut(s) 230, 436, 1637
BspCNI CTCAG 3 cut(s) 82, 294, 649
BspFNI CGCG 2 cut(s) 1033, 1368
BspLI GGNNCC 4 cut(s) 1144, 1204, 1352, 1353
BspMI ACCTGC 1 cut(s) 387
BspQI GCTCTTC 1 cut(s) 207
BspT104I TTCGAA 2 cut(s) 618, 796
BspT107I GGYRCC 2 cut(s) 1142, 1202
BsrDI GCAATG 3 cut(s) 213, 554, 1279
BsrI ACTGG 7 cut(s) 132, 467, 561, 915, 1238, 1351, 1543
BssECI CCNNGG 2 cut(s) 759, 1186
BssMI GATC 1 cut(s) 136
BssSI CACGAG 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 759
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 5 cut(s) 459, 1188, 1200, 1359, 1490
Bst4CI ACNGT 1 cut(s) 1759
Bst6I CTCTTC 1 cut(s) 207
BstBI TTCGAA 2 cut(s) 618, 796
BstC8I GCNNGC 2 cut(s) 115, 1035
BstDEI CTNAG 5 cut(s) 69, 284, 302, 657, 846
BstF5I GGATG 6 cut(s) 818, 845, 1077, 1573, 1601, 1614
BstFNI CGCG 2 cut(s) 1033, 1368
BstHHI GCGC 1 cut(s) 938
BstKTI GATC 1 cut(s) 139
BstMAI GTCTC 2 cut(s) 82, 357
BstMBI GATC 1 cut(s) 136
BstMWI GCNNNNNNNGC 5 cut(s) 203, 935, 1340, 1556, 1598
BstNI CCWGG 5 cut(s) 459, 1188, 1200, 1359, 1490
BstNSI RCATGY 2 cut(s) 59, 869
BstSCI CCNGG 6 cut(s) 457, 1186, 1198, 1357, 1488, 1604
BstSFI CTRYAG 1 cut(s) 1107
BstSLI GKGCMC 1 cut(s) 1129
BstUI CGCG 2 cut(s) 1033, 1368
BstV1I GCAGC 2 cut(s) 1416, 1709
BstV2I GAAGAC 1 cut(s) 805
BsuI GTATCC 2 cut(s) 39, 766
BsuRI GGCC 3 cut(s) 230, 436, 1637
BtsCI GGATG 6 cut(s) 818, 845, 1077, 1573, 1601, 1614
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 3 cut(s) 139, 260, 908
BtuMI TCGCGA 1 cut(s) 1033
BveI ACCTGC 1 cut(s) 387
Cac8I GCNNGC 2 cut(s) 115, 1035
CfoI GCGC 1 cut(s) 938
Cfr13I GGNCC 2 cut(s) 229, 1351
Csp6I GTAC 4 cut(s) 324, 1203, 1416, 1519
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 8 cut(s) 40, 56, 145, 665, 866, 1291, 1552, 1586
CviQI GTAC 4 cut(s) 324, 1203, 1416, 1519
DdeI CTNAG 5 cut(s) 69, 284, 302, 657, 846
DpnI GATC 1 cut(s) 138
DpnII GATC 1 cut(s) 136
DraI TTTAAA 1 cut(s) 990
EaeI YGGCCR 1 cut(s) 1635
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 759
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 1351
Eco88I CYCGRG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 1351
EcoRII CCWGG 5 cut(s) 457, 1186, 1198, 1357, 1488
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 759
ErhI CCWWGG 1 cut(s) 759
FaeI CATG 8 cut(s) 43, 59, 148, 668, 869, 1294, 1555, 1589
FaqI GGGAC 2 cut(s) 1226, 1364
FatI CATG 8 cut(s) 39, 55, 144, 664, 865, 1290, 1551, 1585
FauI CCCGC 1 cut(s) 694
FauNDI CATATG 1 cut(s) 854
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 1405, 1698
FokI GGATG 6 cut(s) 825, 852, 1084, 1580, 1588, 1601
Fsp4HI GCNGC 2 cut(s) 1405, 1698
FspBI CTAG 4 cut(s) 233, 579, 690, 1173
GlaI GCGC 1 cut(s) 937
GluI GCNGC 2 cut(s) 1405, 1698
GsaI CCCAGC 1 cut(s) 1041
GsuI CTGGAG 2 cut(s) 1182, 1341
HaeIII GGCC 3 cut(s) 230, 436, 1637
HapII CCGG 1 cut(s) 1605
HhaI GCGC 1 cut(s) 938
Hin1II CATG 8 cut(s) 43, 59, 148, 668, 869, 1294, 1555, 1589
Hin6I GCGC 1 cut(s) 936
HinP1I GCGC 1 cut(s) 936
HincII GTYRAC 2 cut(s) 520, 1665
HindII GTYRAC 2 cut(s) 520, 1665
HindIII AAGCTT 2 cut(s) 1048, 1058
HpaII CCGG 1 cut(s) 1605
HphI GGTGA 2 cut(s) 1289, 1675
Hpy166II GTNNAC 6 cut(s) 37, 520, 719, 892, 1528, 1665
Hpy188I TCNGA 9 cut(s) 202, 303, 376, 392, 409, 478, 1015, 1066, 1813
Hpy8I GTNNAC 6 cut(s) 37, 520, 719, 892, 1528, 1665
HpyAV CCTTC 5 cut(s) 614, 1448, 1459, 1628, 1682
HpyCH4III ACNGT 1 cut(s) 1759
HpyCH4IV ACGT 1 cut(s) 326
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 935, 1340, 1556, 1598
HpyF3I CTNAG 5 cut(s) 69, 284, 302, 657, 846
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 8 cut(s) 43, 59, 148, 668, 869, 1294, 1555, 1589
HspAI GCGC 1 cut(s) 936
KflI GGGWCCC 1 cut(s) 1351
KpnI GGTACC 1 cut(s) 1206
Kzo9I GATC 1 cut(s) 136
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 862, 1694
Lsp1109I GCAGC 2 cut(s) 1416, 1709
LweI GCATC 2 cut(s) 830, 1610
MaeI CTAG 4 cut(s) 233, 579, 690, 1173
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 5 cut(s) 151, 257, 1159, 1459, 1496
MalI GATC 1 cut(s) 138
MboI GATC 1 cut(s) 136
MboII GAAGA 5 cut(s) 224, 474, 810, 1160, 1226
MfeI CAATTG 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 1129
MlsI TGGCCA 1 cut(s) 1637
MluNI TGGCCA 1 cut(s) 1637
MlyI GAGTC 3 cut(s) 359, 1499, 1660
Mox20I TGGCCA 1 cut(s) 1637
MscI TGGCCA 1 cut(s) 1637
MseI TTAA 5 cut(s) 399, 818, 903, 989, 1178
MslI CAYNNNNRTG 3 cut(s) 347, 811, 857
Msp20I TGGCCA 1 cut(s) 1637
MspA1I CMGCKG 4 cut(s) 656, 724, 732, 1700
MspI CCGG 1 cut(s) 1605
MspR9I CCNGG 6 cut(s) 459, 1188, 1200, 1359, 1490, 1606
MunI CAATTG 1 cut(s) 530
MvaI CCWGG 5 cut(s) 459, 1188, 1200, 1359, 1490
MvnI CGCG 2 cut(s) 1033, 1368
MwoI GCNNNNNNNGC 5 cut(s) 203, 935, 1340, 1556, 1598
NciI CCSGG 1 cut(s) 1606
NdeI CATATG 1 cut(s) 854
NdeII GATC 1 cut(s) 136
NlaIII CATG 8 cut(s) 43, 59, 148, 668, 869, 1294, 1555, 1589
NlaIV GGNNCC 4 cut(s) 1144, 1204, 1352, 1353
NmuCI GTSAC 2 cut(s) 257, 1159
NruI TCGCGA 1 cut(s) 1033
NspI RCATGY 2 cut(s) 59, 869
NspV TTCGAA 2 cut(s) 618, 796
PaeR7I CTCGAG 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 207
PfeI GAWTC 8 cut(s) 387, 479, 615, 1280, 1287, 1646, 1762, 1808
PflFI GACNNNGTC 1 cut(s) 500
PfoI TCCNGGA 2 cut(s) 1357, 1488
PkrI GCNGC 2 cut(s) 1406, 1699
PleI GAGTC 3 cut(s) 359, 1499, 1660
PpsI GAGTC 3 cut(s) 359, 1499, 1660
PpuMI RGGWCCY 1 cut(s) 1351
Psp5II RGGWCCY 1 cut(s) 1351
Psp6I CCWGG 5 cut(s) 457, 1186, 1198, 1357, 1488
PspFI CCCAGC 1 cut(s) 1037
PspGI CCWGG 5 cut(s) 457, 1186, 1198, 1357, 1488
PspN4I GGNNCC 4 cut(s) 1144, 1204, 1352, 1353
PspPI GGNCC 2 cut(s) 229, 1351
PspPPI RGGWCCY 1 cut(s) 1351
PsyI GACNNNGTC 1 cut(s) 500
PvuII CAGCTG 2 cut(s) 656, 724
RruI TCGCGA 1 cut(s) 1033
RsaI GTAC 4 cut(s) 325, 1204, 1417, 1520
RsaNI GTAC 4 cut(s) 324, 1203, 1416, 1519
RseI CAYNNNNRTG 3 cut(s) 347, 811, 857
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 5 cut(s) 399, 818, 903, 989, 1178
SatI GCNGC 2 cut(s) 1405, 1698
Sau3AI GATC 1 cut(s) 136
Sau96I GGNCC 2 cut(s) 229, 1351
SchI GAGTC 3 cut(s) 359, 1499, 1660
ScrFI CCNGG 6 cut(s) 459, 1188, 1200, 1359, 1490, 1606
SduI GDGCHC 1 cut(s) 1129
SfaNI GCATC 2 cut(s) 830, 1610
SfcI CTRYAG 1 cut(s) 1107
Sfr274I CTCGAG 1 cut(s) 237
SfuI TTCGAA 2 cut(s) 618, 796
SinI GGWCC 1 cut(s) 1351
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 3 cut(s) 347, 811, 857
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SsiI CCGC 3 cut(s) 687, 732, 1700
SspI AATATT 2 cut(s) 121, 571
SspMI CTAG 4 cut(s) 233, 579, 690, 1173
StyD4I CCNGG 6 cut(s) 457, 1186, 1198, 1357, 1488, 1604
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 1 cut(s) 1759
TaiI ACGT 1 cut(s) 329
TaqI TCGA 9 cut(s) 238, 294, 416, 443, 618, 796, 964, 1285, 1373
TfiI GAWTC 8 cut(s) 387, 479, 615, 1280, 1287, 1646, 1762, 1808
Tru1I TTAA 5 cut(s) 399, 818, 903, 989, 1178
Tru9I TTAA 5 cut(s) 399, 818, 903, 989, 1178
TscAI CASTG 3 cut(s) 139, 260, 915
TseFI GTSAC 2 cut(s) 257, 1159
TseI GCWGC 2 cut(s) 1404, 1697
Tsp45I GTSAC 2 cut(s) 257, 1159
TspDTI ATGAA 5 cut(s) 194, 524, 653, 1326, 1659
TspRI CASTG 3 cut(s) 139, 260, 915
Tth111I GACNNNGTC 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 1351
XapI RAATTY 5 cut(s) 88, 278, 308, 410, 991
XbaI TCTAGA 1 cut(s) 578
XceI RCATGY 2 cut(s) 59, 869
XhoI CTCGAG 1 cut(s) 237
XmiI GTMKAC 1 cut(s) 36
XspI CTAG 4 cut(s) 233, 579, 690, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.