RchiOBHm_Chr4g0384761

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015256
Physical Location & Seq
Reverse (-)
96846 .. 98893
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1824 bp
ATGAAATCAATATACACAAGCTACAGAAACAGGAGTATGCATCATGTGATCAGGAGTCGGTCCGCGTCTTCTGGCCTTTGCTGCATCAAAATCAAAGACTTGGTCTCTAAAGGATTATATGATCAAACCCTTCAACTTTACAAAGACGAGCTCCATTCTTATGGCCTCCTCCACGCAAACACCTCCATCCTTCCTTCAATCATCAAAGCATGCTCCTCCCACCATGGGCATGGGCTTCAACTTCACTGCATGGCTCTCAAGTCAGGCTCCAACTCAGACTCCGTTGTATCCAATTCTCTCATCTCCATGTATGCCAAACTCGCACTTGTTGGAGCAGCACGCCGGGTGTTTGATGAAATGCCTGAAAGAGACACCATCACCTGGAATTCCATGATAAATTGCTACATACAAAATGGGCATCTTGCAAAAGCACTTGATGTGCTTAAACAAATGTATTTGAATGGTTTTACACTGAAAGCGGAGTTGATTGCCAGCATCATATCAGTTTGTGCAAGGACAAGAAAGTTTAGACTAGGGAGACAGATTCATGGCCTCCTTGTTGTCCATGACGAGATTGAAGAGTCGGTGTTCTTGTCAACGGCTCTGCTTGATTTGTATATGAGGTGTCACGAAACTTTGATGGCCTTCCATGTTTTCTGTCACATGCAAGTGAAAAATGAAGTCTCTTGGACTGCCATGATTTCGGGATGCATTGCTAATCGCAGCTATGGGATGGCAATGCATTGCTTCCGGGAAATGCAGGTTGAAGGATTGAAACCCAATCGAGTCACTATGCTTGCCATTTTACCAGCTTGTGCCGAGTTGGGAGACATAGGACATGGGAAGGAAATTCATGCCTACACTTTTCGTCATGGGTTTGAATCAGATCACCATTGCTCAGCAGCTCTCATACACATGTACTGCAAGCGCGGGAAAGAATTGCGTCCTGCCAAGCTAATTTTCGACAAGGTATCTCTTAAAGATGTTGTTATGTGGAGTTTGATGATTGGGAGTTATTCTCAATGTGGGGATTTCGTGAAAGCTTTGCAACTTTTTAGTCAAATGCGAGCTCAAGGAATTGATCCAAACTCTGTAACGTTACTGGCCCTCATTTCCGCCTGCACCACACTTTCTTCTCTACAGATCGGATGTGGAATCCATAGCTATGTCTTGAAATCTGGGTTAAATTCTGACATTTTCATTGGGAATGCACTTATCAATATGTATGCAAAGTGTGGTTGTCTCACGGATTCACATGAAATTTTCAAGGAGATGCCAAGTATAGATTCTGTCTCCTGGAGCACAATGATTGAGAGCTATGGCCTTCATGGTCGTGGTAATGAAGCTTTGAAACTCTTTCTTGAGATGCAAGATAGAGGGATAGAGCCTGATGCGGTCGCTTTCCTCTCAGTTTTATCTGCTTGTAACCATGCTGGCCTGGTGGAAGAAGGCAAGAAGATCTTTAAACTTATGAGAGAAGATAGTAAAATAACTGTATCTTTAGAGCATTATGCTTGCTATATTAATCTTCTTGGAAAGTTTGGAAAGCTCGAAGATGCTTGTGAGATTGTACGATCAATGCCCATGAAACCAAGTAGTAGAATTTGGAGTTCTTTGGTTACAGCTTGCAAGGTTCACGGAAGGTTAGAAATAGCAGAATCCTTGGCGCATCATCTTGTTAGAAAGGAGCCTCAGAACGCTGCCAACTATGCCTTACTGAGCATGGTTCATGCTGAAGCTGGTAACTGGCTTGGCGTGGAGGAGGTGAGGAGAGTCATGAGAGTACACAGATTAAGGAAATGCTACGGATTCAGCAGAATTTAG

Protein Analysis

607

Amino Acids

67.92

Weight (kDa)

8.74

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 123 - 156 7.5e-07 PPR repeat family
PPR PF01535 126 - 155 4.2e-07 PPR repeat
PPR_2 PF13041 226 - 273 1.1e-08 PPR repeat family
PPR_2 PF13041 327 - 374 1.1e-10 PPR repeat family
PPR PF01535 330 - 360 1.3e-06 PPR repeat
PPR_2 PF13041 429 - 475 1.3e-10 PPR repeat family
PPR PF01535 431 - 461 5.7e-08 PPR repeat
E_motif PF20431 546 - 607 2.4e-09 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31070 AT4G31070
fragaria_vesca FvH4_4g00140
malus_domestica MD04G1001600.v1.1
prunus_persica Prupe.1G001600_v2.0.a1
pyrus_communis pycom04g00220
rosa_chinensis RchiOBHm_Chr4g0384761
rosa_laevigata RLG00000010274
rosa_roxburghii Rroxscaffold_5G00332820
rosa_rugosa Rorug03G0297500
rosa_samantha Rh4AG001000 Rh4BG000900 Rh4CG000800 Rh4DG000900
rosa_wichuraiana Rw0G021980 Rw4G000790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 751
AccB7I CCANNNNNTGG 1 cut(s) 381
AccII CGCG 2 cut(s) 65, 930
AciI CCGC 5 cut(s) 63, 479, 930, 1116, 1394
AclI AACGTT 1 cut(s) 1097
AclWI GGATC 1 cut(s) 1076
AcsI RAATTY 6 cut(s) 385, 849, 1186, 1260, 1602, 1818
AcuI CTGAAG 1 cut(s) 1755
AfaI GTAC 3 cut(s) 920, 1572, 1785
AfiI CCNNNNNNNGG 2 cut(s) 225, 381
AflIII ACRYGT 1 cut(s) 915
AjnI CCWGG 3 cut(s) 380, 1295, 1437
AjuI GAANNNNNNNTTGG 4 cut(s) 944, 976, 1270, 1302
Alw21I GWGCWC 3 cut(s) 153, 1072, 1304
Alw26I GTCTC 7 cut(s) 109, 363, 532, 688, 822, 1247, 1297
AlwI GGATC 1 cut(s) 1076
AoxI GGCC 7 cut(s) 73, 163, 550, 642, 1104, 1321, 1435
ApeKI GCWGC 5 cut(s) 81, 335, 723, 902, 1700
ApoI RAATTY 6 cut(s) 385, 849, 1186, 1260, 1602, 1818
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AseI ATTAAT 1 cut(s) 1524
AspLEI GCGC 2 cut(s) 930, 1669
AspS9I GGNCC 2 cut(s) 60, 1105
AsuC2I CCSGG 2 cut(s) 344, 752
AsuHPI GGTGA 3 cut(s) 370, 881, 1777
AvaII GGWCC 1 cut(s) 60
BanII GRGCYC 2 cut(s) 153, 1072
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 3 cut(s) 153, 1072, 1304
BbvI GCAGC 5 cut(s) 68, 347, 735, 914, 1687
BccI CCATC 4 cut(s) 194, 383, 634, 727
BceAI ACGGC 1 cut(s) 615
BciT130I CCWGG 3 cut(s) 382, 1297, 1439
BciVI GTATCC 1 cut(s) 298
BclI TGATCA 2 cut(s) 48, 121
BcnI CCSGG 2 cut(s) 344, 752
BcoDI GTCTC 7 cut(s) 109, 363, 532, 688, 822, 1247, 1297
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 2 cut(s) 22, 1139
BfuAI ACCTGC 1 cut(s) 751
BfuI GTATCC 1 cut(s) 298
BglII AGATCT 1 cut(s) 1458
BisI GCNGC 5 cut(s) 82, 336, 724, 903, 1701
BlpI GCTNAGC 1 cut(s) 898
BlsI GCNGC 5 cut(s) 83, 337, 725, 904, 1702
Bme1390I CCNGG 5 cut(s) 344, 382, 752, 1297, 1439
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 60, 1105
BmiI GGNNCC 2 cut(s) 268, 1689
BmrFI CCNGG 5 cut(s) 344, 382, 752, 1297, 1439
BpiI GAAGAC 1 cut(s) 60
BplI GAGNNNNNCTC 2 cut(s) 1003, 1035
BpmI CTGGAG 1 cut(s) 1318
Bpu1102I GCTNAGC 1 cut(s) 898
BpuEI CTTGAG 3 cut(s) 242, 1056, 1382
BpuMI CCSGG 2 cut(s) 344, 752
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 223, 1662
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 381
Bse1I ACTGG 2 cut(s) 1107, 1751
Bse3DI GCAATG 4 cut(s) 711, 742, 744, 892
BseBI CCWGG 3 cut(s) 382, 1297, 1439
BseDI CCNNGG 2 cut(s) 223, 1662
BseGI GGATG 4 cut(s) 186, 713, 738, 1154
BseLI CCNNNNNNNGG 2 cut(s) 225, 381
BseMI GCAATG 4 cut(s) 711, 742, 744, 892
BseMII CTCAG 5 cut(s) 288, 912, 1422, 1706, 1709
BseNI ACTGG 2 cut(s) 1107, 1751
BseRI GAGGAG 4 cut(s) 158, 205, 1775, 1783
BseXI GCAGC 5 cut(s) 68, 347, 735, 914, 1687
BsgI GTGCAG 1 cut(s) 1105
Bsh1236I CGCG 2 cut(s) 65, 930
Bsh1285I CGRYCG 1 cut(s) 1398
BshFI GGCC 7 cut(s) 75, 165, 552, 644, 1106, 1323, 1437
BsiEI CGRYCG 1 cut(s) 1398
BsiHKAI GWGCWC 3 cut(s) 153, 1072, 1304
BsiSI CCGG 2 cut(s) 343, 751
BslI CCNNNNNNNGG 2 cut(s) 225, 381
BsmAI GTCTC 7 cut(s) 109, 363, 532, 688, 822, 1247, 1297
BsmI GAATGC 1 cut(s) 1213
BsnI GGCC 7 cut(s) 75, 165, 552, 644, 1106, 1323, 1437
Bso31I GGTCTC 1 cut(s) 109
Bsp1286I GDGCHC 3 cut(s) 153, 1072, 1304
Bsp143I GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
Bsp1720I GCTNAGC 1 cut(s) 898
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 5 cut(s) 63, 479, 930, 1116, 1394
BspANI GGCC 7 cut(s) 75, 165, 552, 644, 1106, 1323, 1437
BspCNI CTCAG 5 cut(s) 287, 911, 1421, 1705, 1710
BspFNI CGCG 2 cut(s) 65, 930
BspHI TCATGA 1 cut(s) 1776
BspLI GGNNCC 2 cut(s) 268, 1689
BspMI ACCTGC 1 cut(s) 751
BspPI GGATC 1 cut(s) 1076
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 4 cut(s) 711, 742, 744, 892
BsrI ACTGG 2 cut(s) 1107, 1751
BssECI CCNNGG 2 cut(s) 223, 1662
BssMI GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
BssT1I CCWWGG 2 cut(s) 223, 1662
Bst2UI CCWGG 3 cut(s) 382, 1297, 1439
Bst4CI ACNGT 1 cut(s) 1495
Bst6I CTCTTC 1 cut(s) 573
BstDEI CTNAG 5 cut(s) 274, 898, 1408, 1692, 1718
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 4 cut(s) 186, 713, 738, 1154
BstFNI CGCG 2 cut(s) 65, 930
BstHHI GCGC 2 cut(s) 930, 1669
BstKTI GATC 7 cut(s) 51, 124, 889, 1084, 1146, 1461, 1577
BstMAI GTCTC 7 cut(s) 109, 363, 532, 688, 822, 1247, 1297
BstMBI GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
BstMCI CGRYCG 1 cut(s) 1398
BstMWI GCNNNNNNNGC 3 cut(s) 81, 320, 1709
BstNI CCWGG 3 cut(s) 382, 1297, 1439
BstNSI RCATGY 3 cut(s) 213, 667, 919
BstSCI CCNGG 5 cut(s) 342, 380, 750, 1295, 1437
BstSFI CTRYAG 2 cut(s) 22, 1139
BstUI CGCG 2 cut(s) 65, 930
BstV1I GCAGC 5 cut(s) 68, 347, 735, 914, 1687
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 1458
BstXI CCANNNNNNTGG 2 cut(s) 161, 230
BstYI RGATCY 1 cut(s) 1458
BsuI GTATCC 1 cut(s) 298
BsuRI GGCC 7 cut(s) 75, 165, 552, 644, 1106, 1323, 1437
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 4 cut(s) 186, 713, 738, 1154
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 2 cut(s) 244, 470
BveI ACCTGC 1 cut(s) 751
CciI TCATGA 1 cut(s) 1776
CfoI GCGC 2 cut(s) 930, 1669
Cfr13I GGNCC 2 cut(s) 60, 1105
CpoI CGGWCCG 1 cut(s) 60
CseI GACGC 2 cut(s) 54, 932
Csp6I GTAC 3 cut(s) 919, 1571, 1784
CspI CGGWCCG 1 cut(s) 60
CviQI GTAC 3 cut(s) 919, 1571, 1784
DdeI CTNAG 5 cut(s) 274, 898, 1408, 1692, 1718
DpnI GATC 7 cut(s) 50, 123, 888, 1083, 1145, 1460, 1576
DpnII GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
DraI TTTAAA 1 cut(s) 1465
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
EciI GGCGGA 1 cut(s) 1105
Ecl136II GAGCTC 2 cut(s) 151, 1070
Eco130I CCWWGG 2 cut(s) 223, 1662
Eco24I GRGCYC 2 cut(s) 153, 1072
Eco31I GGTCTC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 60
Eco53kI GAGCTC 2 cut(s) 151, 1070
Eco57I CTGAAG 1 cut(s) 1755
EcoICRI GAGCTC 2 cut(s) 151, 1070
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 380, 1295, 1437
EcoT14I CCWWGG 2 cut(s) 223, 1662
EcoT22I ATGCAT 3 cut(s) 42, 713, 744
EcoT38I GRGCYC 2 cut(s) 153, 1072
ErhI CCWWGG 2 cut(s) 223, 1662
FalI AAGNNNNNCTT 2 cut(s) 1445, 1477
FauI CCCGC 1 cut(s) 923
FbaI TGATCA 2 cut(s) 48, 121
Fnu4HI GCNGC 5 cut(s) 82, 336, 724, 903, 1701
FokI GGATG 4 cut(s) 173, 720, 745, 1161
FriOI GRGCYC 2 cut(s) 153, 1072
Fsp4HI GCNGC 5 cut(s) 82, 336, 724, 903, 1701
FspBI CTAG 1 cut(s) 533
GlaI GCGC 2 cut(s) 929, 1668
GluI GCNGC 5 cut(s) 82, 336, 724, 903, 1701
GsuI CTGGAG 1 cut(s) 1318
HaeIII GGCC 7 cut(s) 75, 165, 552, 644, 1106, 1323, 1437
HapII CCGG 2 cut(s) 343, 751
HgaI GACGC 2 cut(s) 54, 932
HhaI GCGC 2 cut(s) 930, 1669
Hin6I GCGC 2 cut(s) 928, 1667
HinP1I GCGC 2 cut(s) 928, 1667
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HindIII AAGCTT 2 cut(s) 1041, 1344
HpaII CCGG 2 cut(s) 343, 751
HphI GGTGA 3 cut(s) 370, 881, 1777
Hpy166II GTNNAC 3 cut(s) 597, 1636, 1786
Hpy188I TCNGA 5 cut(s) 277, 886, 1148, 1192, 1695
Hpy188III TCNNGA 7 cut(s) 52, 629, 705, 1036, 1171, 1361, 1777
Hpy8I GTNNAC 3 cut(s) 597, 1636, 1786
HpyAV CCTTC 9 cut(s) 140, 200, 204, 655, 761, 838, 1334, 1442, 1635
HpyCH4III ACNGT 1 cut(s) 1495
HpyCH4IV ACGT 1 cut(s) 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 320, 1709
HpyF3I CTNAG 5 cut(s) 274, 898, 1408, 1692, 1718
HpySE526I ACGT 1 cut(s) 1097
HspAI GCGC 2 cut(s) 928, 1667
Ksp22I TGATCA 2 cut(s) 48, 121
Kzo9I GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
LmnI GCTCC 6 cut(s) 156, 218, 272, 332, 1299, 1687
Lsp1109I GCAGC 5 cut(s) 68, 347, 735, 914, 1687
MaeI CTAG 1 cut(s) 533
MaeII ACGT 1 cut(s) 1097
MaeIII GTNAC 8 cut(s) 626, 659, 787, 1093, 1098, 1424, 1618, 1742
MalI GATC 7 cut(s) 50, 123, 888, 1083, 1145, 1460, 1576
MboI GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
MboII GAAGA 8 cut(s) 60, 590, 1125, 1457, 1468, 1490, 1520, 1565
MflI RGATCY 1 cut(s) 1458
MhlI GDGCHC 3 cut(s) 153, 1072, 1304
MlyI GAGTC 5 cut(s) 64, 272, 590, 795, 1782
MmeI TCCRAC 2 cut(s) 294, 310
Mph1103I ATGCAT 3 cut(s) 42, 713, 744
MseI TTAA 6 cut(s) 444, 978, 1184, 1464, 1524, 1793
MslI CAYNNNNRTG 7 cut(s) 159, 228, 305, 668, 914, 1164, 1332
MspI CCGG 2 cut(s) 343, 751
MspR9I CCNGG 5 cut(s) 344, 382, 752, 1297, 1439
Mva1269I GAATGC 1 cut(s) 1213
MvaI CCWGG 3 cut(s) 382, 1297, 1439
MvnI CGCG 2 cut(s) 65, 930
MwoI GCNNNNNNNGC 3 cut(s) 81, 320, 1709
NciI CCSGG 2 cut(s) 344, 752
NcoI CCATGG 1 cut(s) 223
NdeII GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
NlaIV GGNNCC 2 cut(s) 268, 1689
NmeAIII GCCGAG 1 cut(s) 844
NmuCI GTSAC 3 cut(s) 626, 659, 787
NsiI ATGCAT 3 cut(s) 42, 713, 744
NspI RCATGY 3 cut(s) 213, 667, 919
PaeI GCATGC 1 cut(s) 213
PagI TCATGA 1 cut(s) 1776
PciI ACATGT 1 cut(s) 915
PctI GAATGC 1 cut(s) 1213
PfeI GAWTC 7 cut(s) 544, 881, 1155, 1250, 1286, 1658, 1809
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 381
PfoI TCCNGGA 2 cut(s) 750, 1295
PkrI GCNGC 5 cut(s) 83, 337, 725, 904, 1702
PleI GAGTC 5 cut(s) 63, 272, 589, 794, 1781
PpsI GAGTC 5 cut(s) 63, 272, 589, 794, 1781
PscI ACATGT 1 cut(s) 915
PshBI ATTAAT 1 cut(s) 1524
Psp124BI GAGCTC 2 cut(s) 153, 1072
Psp1406I AACGTT 1 cut(s) 1097
Psp6I CCWGG 3 cut(s) 380, 1295, 1437
PspGI CCWGG 3 cut(s) 380, 1295, 1437
PspN4I GGNNCC 2 cut(s) 268, 1689
PspPI GGNCC 2 cut(s) 60, 1105
PsuI RGATCY 1 cut(s) 1458
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 3 cut(s) 920, 1572, 1785
RsaNI GTAC 3 cut(s) 919, 1571, 1784
RseI CAYNNNNRTG 7 cut(s) 159, 228, 305, 668, 914, 1164, 1332
Rsr2I CGGWCCG 1 cut(s) 60
RsrII CGGWCCG 1 cut(s) 60
SacI GAGCTC 2 cut(s) 153, 1072
SaqAI TTAA 6 cut(s) 444, 978, 1184, 1464, 1524, 1793
SatI GCNGC 5 cut(s) 82, 336, 724, 903, 1701
Sau3AI GATC 7 cut(s) 48, 121, 886, 1081, 1143, 1458, 1574
Sau96I GGNCC 2 cut(s) 60, 1105
SchI GAGTC 5 cut(s) 64, 272, 590, 795, 1782
ScrFI CCNGG 5 cut(s) 344, 382, 752, 1297, 1439
SduI GDGCHC 3 cut(s) 153, 1072, 1304
SfcI CTRYAG 2 cut(s) 22, 1139
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 7 cut(s) 159, 228, 305, 668, 914, 1164, 1332
SmlI CTYRAG 3 cut(s) 257, 1071, 1361
SmoI CTYRAG 3 cut(s) 257, 1071, 1361
SphI GCATGC 1 cut(s) 213
SsiI CCGC 5 cut(s) 63, 479, 930, 1116, 1394
SspMI CTAG 1 cut(s) 533
SstI GAGCTC 2 cut(s) 153, 1072
StyD4I CCNGG 5 cut(s) 342, 380, 750, 1295, 1437
StyI CCWWGG 2 cut(s) 223, 1662
TaaI ACNGT 1 cut(s) 1495
TaiI ACGT 1 cut(s) 1100
TaqI TCGA 3 cut(s) 784, 963, 1551
TaqII GACCGA 1 cut(s) 48
TatI WGTACW 2 cut(s) 918, 1783
TfiI GAWTC 7 cut(s) 544, 881, 1155, 1250, 1286, 1658, 1809
Tru1I TTAA 6 cut(s) 444, 978, 1184, 1464, 1524, 1793
Tru9I TTAA 6 cut(s) 444, 978, 1184, 1464, 1524, 1793
TscAI CASTG 2 cut(s) 251, 477
TseFI GTSAC 3 cut(s) 626, 659, 787
TseI GCWGC 5 cut(s) 81, 335, 723, 902, 1700
Tsp45I GTSAC 3 cut(s) 626, 659, 787
TspGWI ACGGA 4 cut(s) 271, 1262, 1653, 1821
TspRI CASTG 2 cut(s) 251, 477
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 60
VspI ATTAAT 1 cut(s) 1524
XapI RAATTY 6 cut(s) 385, 849, 1186, 1260, 1602, 1818
XceI RCATGY 3 cut(s) 213, 667, 919
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XspI CTAG 1 cut(s) 533
Zsp2I ATGCAT 3 cut(s) 42, 713, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.