RchiOBHm_Chr3g0482831

Regulator of nonsense transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007975
Physical Location & Seq
Reverse (-)
28889617 .. 28900442
10826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3576 bp
ATGGCAGTCAATACGAGTAGTGAGATGGATCACAATGAGGAGGAATCCGGTGGTGGTGCCGGAGCTGAACCTCATGGGAAGCAAGATGATGAGGAAGCCGTTGCTCGGCTTGAGGAAATGAAGAAATCAATCGAGTCAAAGATGGCTCTCCGTCAAAGCAATTTGAACCCCGAGAGGCCTGACTCGGGATTTCTGAGGACATTGGATTCCAGCATTAAACGCAACACAGCAGTTATTAAAAAACTGAAGCAGATAAATGAAGAGCAGCGGGAAGGGCTGATGGATGACTTGAGAAGTGTCAACCTTAGCAAATTTGTCAGTGAAGCTGTTACTGCTATTTGTGACGCCAAACTTAGATCGTCGGACATACAAGCAGCAGTTCAGATCTGCTCTCTGCTTCATCAAAGATACAAAGACTTCTCCCCGACCCTAGTTCAAGGTCTTTTGAAAGTCTTCTTTCCTGGAAAGTCTGGGGATGACCCAGATGCAGATAGGAACCTGAGAGCCATGAAGAAGCGTAGCACTTTGAAACTCCTTTTGGAACTTTACTTTGTTGGAGTTATAGAAGATGGTGGTATTTTTGTGAATATTATTAAAGATCTTACCAGTGGAGAACACTTAAAGGACCGGGATACTACTCAGACAAATTTGACTCTTCTTGCTAGTTTTGCTCGGCAGGGAAGAATGTTTCTTGGTCTTCCACTCTCTGGGCCAGAAATACATGAAGAGTTTTTTAAGGGCCTTAATATCACACCAGATCAAAAGAAGTTCTTCAAGAAGGCATTTCAAGCATATTATGAAGCTGCAGCTGAACTGCTTCAGTCTGAGCATACTTCACTTCGCCAAATGGAGCATGAAAATGCAAGAATCCTTAATGCTAAAGGAGAGCTCAGTGATGATAGTGCCTCTTCATATGAAAAGCTGCGGAAATCTTATGACCATTTGTACCGCAATGTCTCAGCTTTAGCAGAAGCACTTGATATGCAGCCTCCAGTAATGCCAGAGGATGGTCACACAACTAGGGTTACTAGCGGAGAGGATGCTTCATCTCCAGCTGCTGGAAAAGATTCTTCTGTACTTGAAGCCATATGGGACGATGAAGATACTAGGGCTTTCTATGAATGTTTGCCAGATCTTAGAGCATTTGTTCCAGCTGTGTTACTGGGAGAAGCAGAGTCTAAAGTCAATGAGCAATCTGCAAAGACCCAAGAGCAACCAACTGAACCTGCGCCTGAATTAGACCAGAGTCAACAGGCTACAGAAGATGCTGCGGAATCATCTGCAGATGTTGGTGCTTTACAAGAAGGAAAAATCAGAGAGAAAGGAAAGGAGAAGGAAGAAAAAGATAAAGACAAGATTAAAGATGCAGACAAAGAGAAGGGTGACAAGAAGGGAGAGAATGAAAAGGAGAAACTAAAAAGTATCGAAGGGACGAATTTGGATGCTTTACTGCAGAGGCTCCCTGGTTGTGTGAGCCGTGACCTCATTGACCAATTGACTGTAGAGTTTTGTTACCTAAATTCTAAAGCAAATCGGAAAAAACTTGTGAGGTCAGTGTTTAATGTCCCCAGGACATCGTTGGAGTTACTGCCGTACTACTCACGCATGGTTGCCACTCTGTCGACTTGTATGAAGGATGTATCTTCCATGCTCCTGGCTATGTTGGAGGAGGAGTTCAATTTCTTAATAAATAAAAAGGATCAAATGAACATTGAAACAAAGATTAGGAATATTAGATTTATTGGAGAACTCTGCAAGTTCAGAATTGCACCAGCTGGTCTTGTGTTTAGTTGTTTAAAGGCTTGTCTAGATGATTTTTCTCATCATAATATTGATGTTGCTTGCAATCTTCTTGAGACTTGTGGCCGTTTTCTGTATCGCTCTCCTGAAACTACTGTGCGGATGGCTAACATGTTGGAAATATTAATGCGCTTAAAGAATGTAAAAAATTTGGATCCTCGGCACAGCACCCTTGTTGAAAATGCTTATTACCTGTGCAAACCGCCTGAGAGATCCGCACGAGTTGCTAAAGTCCGTCCTCCATTGCATCAGTATATAAGAAAGTTGCTATTCTCAGATCTTGATAAATCTACCCTTGAGCATGTGCTGAGGCAATTGCGTAAACTACCATGGGGTGAATGTGAGCCATATCTTTTGAAATGCTTTATGAAGGTTCACAAAGGGAAGTACGGTCAGATCCATTTGATTGCTTCTCTCACTGCTGGCCTGAGTCGCTATCATGATGAATTTGCTGTCTCTGTTGTTGACGAGGTTTTAGAGGAGATCCGGCTTGGCCTTGAATTAAATGAGTACGGAATGCAGCAAAGACGAATTGCCCATATGCGATTCCTAGGAGAGTTATACAACTATGAACATGTAGATTCTTCTGTTATATTTGAGACTCTATACTTGATTCTCATCTTTGGCCATGGCACAACAGAGCAAGATGTATTAGATCCACCAGAAGATTGTTTCCGCATAAGGATGGTTATTACTCTTCTCGAAACTTGTGGACATTACTTTGACCGAGGCTCTTCTAAGAGGAAACTTGATCGCTTCTTGATGCACTTTCAGAGATATATACTAAGCAAAGGTGTACTACCACTAGATGTTGAGTTTGACTTACAGGACTTATTTGCAGAATTGCGTCCCAATATGACTCGATACTCATCCCTTGAAGAAGTTAATGCAGCTCTGGTAGAGCTTGAGGAGCACTCGGAGAAGGCCAATAATGAGAAGCACTCAGACACTGAAAAGTCTTCAAGGAGGACAACCTCCAATAAAATGTCAGTGAATGAACAGGGTGTTGCGAATGGTACAGAGGGAAATGGGGCGGTGCATGAAGACCATAGAGATAGTGACAGTGATTCAGGAAGTGGCACTGTTGATCCAGACAGACATGAAGAAGAATTGGATGAAGAGAATCATGATGATGGATCAGAGAGTGAAGAGGAGGATGATGATGGGGGTGGACCTGCTTCTGACGAGGAGGATGAAGTCCATGTCAGACAGAAGGTTGCAGAGGTGGATCCTCAGGAAGAAGCAGATTTTGAGCTGGATCTCAAGGCTGTTATGCAGGAAAGCATGGAGCAACGAAGGCAAGAGCTGCGCGGTCGGCCTACATTGAATATGATGATACCAATGAATGTGTTTGAGGGGTCCATCAAGGACCATCATGGACGTGTTGGAGGGGACAGTGGTGATGAGGGGGATGAGGAGTCTGGAGGAAGCAAGGATGTCCAAGTCAAAGTTCTCGTGAAGCGTGGTAACAAGCAACAAACAAAACAGATGTACATCCCTCGTGATTGCTCCCTTATACAGAGCACAAAACAGAAAGAAGCGGCAGAGCTTGAAGAGAAACAAGACATCAAGAGGCTAGTCTTGGAATATAATGATAGAGAAGAGGAGGAACTTAATGGATTAGGGAACCAGACTATGAACTATGCGCAGAGTGGTGGCAATAGATTTGCTGGTCGTGGCAGCACTTGGGAAGGAACTAGCAGCAGAGGTGGTGGACCACGTCGATATCACAGTAGTTATTCTGGTGCTGGAGTTTATTACAGCAGGAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0031503 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034655 GO:0035145 GO:0035690 GO:0035770 GO:0036464 GO:0042162 GO:0042221 GO:0042493 GO:0042742 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046677 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071426 GO:0071427 GO:0071446 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
MIF4G Upf2

Protein Analysis

1191

Amino Acids

134.62

Weight (kDa)

5.24

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 73 - 268 1.1e-07 MIF4G domain
MIF4G PF02854 483 - 669 1.1e-40 MIF4G domain
MIF4G PF02854 685 - 880 2.2e-44 MIF4G domain
Upf2 PF04050 995 - 1129 1.2e-33 Up-frameshift suppressor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3447
Acc36I ACCTGC 2 cut(s) 1234, 2983
AccB1I GGYRCC 1 cut(s) 56
AccB7I CCANNNNNTGG 3 cut(s) 707, 1007, 1058
AccI GTMKAC 1 cut(s) 1622
AccII CGCG 1 cut(s) 3111
AcoI YGGCCR 2 cut(s) 1864, 2425
AcsI RAATTY 6 cut(s) 311, 646, 1435, 1519, 1948, 2246
AcuI CTGAAG 2 cut(s) 266, 803
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 8 cut(s) 947, 1077, 1595, 2189, 2312, 2598, 2818, 3293
AfiI CCNNNNNNNGG 5 cut(s) 105, 185, 707, 1007, 1058
AflIII ACRYGT 3 cut(s) 1911, 2374, 3181
AjiI CACGTC 2 cut(s) 3182, 3521
AjnI CCWGG 4 cut(s) 460, 1462, 1568, 1653
AjuI GAANNNNNNNTTGG 4 cut(s) 521, 553, 3234, 3266
AloI GAACNNNNNNTCC 2 cut(s) 1658, 1690
Alw21I GWGCWC 3 cut(s) 891, 2715, 3326
Alw26I GTCTC 4 cut(s) 961, 1850, 2260, 2393
AlwNI CAGNNNCTG 2 cut(s) 1058, 2750
Ama87I CYCGRG 2 cut(s) 170, 184
AoxI GGCC 9 cut(s) 176, 710, 739, 1864, 2224, 2293, 2425, 2724, 3116
ApoI RAATTY 6 cut(s) 311, 646, 1435, 1519, 1948, 2246
AseI ATTAAT 1 cut(s) 1925
Asp700I GAANNNNTTC 4 cut(s) 452, 770, 816, 1066
AspA2I CCTAGG 1 cut(s) 2350
AspLEI GCGC 4 cut(s) 1231, 1932, 3111, 3448
AspS9I GGNCC 7 cut(s) 625, 710, 739, 2972, 3159, 3169, 3515
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 3 cut(s) 1394, 2147, 3212
AvaI CYCGRG 2 cut(s) 170, 184
AvaII GGWCC 5 cut(s) 625, 2972, 3159, 3169, 3515
AvrII CCTAGG 1 cut(s) 2350
AxyI CCTNAGG 1 cut(s) 3033
BalI TGGCCA 1 cut(s) 2427
BamHI GGATCC 2 cut(s) 1954, 3028
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 891
BauI CACGAG 3 cut(s) 2019, 3254, 3300
BbsI GAAGAC 4 cut(s) 445, 689, 2751, 2850
Bbv12I GWGCWC 3 cut(s) 891, 2715, 3326
BbvCI CCTCAGC 1 cut(s) 2108
BceAI ACGGC 4 cut(s) 83, 1461, 1576, 1851
BciT130I CCWGG 4 cut(s) 462, 1464, 1570, 1655
BciVI GTATCC 1 cut(s) 625
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 4 cut(s) 961, 1850, 2260, 2393
BfmI CTRYAG 5 cut(s) 804, 1257, 1281, 1451, 1500
BfuAI ACCTGC 2 cut(s) 1234, 2983
BfuI GTATCC 1 cut(s) 625
BglII AGATCT 4 cut(s) 384, 598, 1132, 2077
BlnI CCTAGG 1 cut(s) 2350
Bme1390I CCNGG 5 cut(s) 462, 629, 1464, 1570, 1655
Bme18I GGWCC 5 cut(s) 625, 2972, 3159, 3169, 3515
BmeT110I CYCGRG 2 cut(s) 170, 184
BmgBI CACGTC 2 cut(s) 3182, 3521
BmgT120I GGNCC 7 cut(s) 625, 710, 739, 2972, 3159, 3169, 3515
BmiI GGNNCC 7 cut(s) 58, 497, 1460, 1956, 3030, 3160, 3428
BmrFI CCNGG 5 cut(s) 462, 629, 1464, 1570, 1655
BmrI ACTGGG 1 cut(s) 1172
BmsI GCATC 7 cut(s) 475, 1030, 1255, 1354, 1432, 2056, 2553
BmuI ACTGGG 1 cut(s) 1172
BoxI GACNNNNGTC 1 cut(s) 1245
BpiI GAAGAC 4 cut(s) 445, 689, 2751, 2850
BplI GAGNNNNNCTC 4 cut(s) 2699, 2731, 2726, 2758
BpmI CTGGAG 4 cut(s) 975, 1035, 3243, 3570
Bpu10I CCTNAGC 2 cut(s) 305, 2108
BpuEI CTTGAG 6 cut(s) 131, 310, 1874, 2117, 2726, 3047
BpuMI CCSGG 1 cut(s) 629
BsaBI GATNNNNATC 3 cut(s) 2417, 2668, 3293
BsaHI GRCGYC 1 cut(s) 345
BsaJI CCNNGG 7 cut(s) 1462, 1568, 1958, 2129, 2350, 2428, 2527
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 1658, 1688
Bsc4I CCNNNNNNNGG 5 cut(s) 105, 185, 707, 1007, 1058
Bse1I ACTGG 3 cut(s) 606, 992, 1167
Bse21I CCTNAGG 1 cut(s) 3033
Bse3DI GCAATG 2 cut(s) 958, 2042
Bse8I GATNNNNATC 3 cut(s) 2417, 2668, 3293
BseBI CCWGG 4 cut(s) 462, 1464, 1570, 1655
BseDI CCNNGG 7 cut(s) 1462, 1568, 1958, 2129, 2350, 2428, 2527
BseJI GATNNNNATC 3 cut(s) 2417, 2668, 3293
BseLI CCNNNNNNNGG 5 cut(s) 105, 185, 707, 1007, 1058
BseMI GCAATG 2 cut(s) 958, 2042
BseNI ACTGG 3 cut(s) 606, 992, 1167
BseRI GAGGAG 9 cut(s) 53, 1682, 1685, 2294, 2723, 2966, 3002, 3230, 3419
Bsh1236I CGCG 1 cut(s) 3111
Bsh1285I CGRYCG 1 cut(s) 3115
BshFI GGCC 9 cut(s) 178, 712, 741, 1866, 2226, 2295, 2427, 2726, 3118
BshNI GGYRCC 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 3115
BsiHKAI GWGCWC 3 cut(s) 891, 2715, 3326
BsiHKCI CYCGRG 2 cut(s) 170, 184
BsiSI CCGG 4 cut(s) 48, 60, 628, 2287
BslFI GGGAC 5 cut(s) 1106, 1444, 1550, 2634, 3206
BslI CCNNNNNNNGG 5 cut(s) 105, 185, 707, 1007, 1058
BsmAI GTCTC 4 cut(s) 961, 1850, 2260, 2393
BsmFI GGGAC 5 cut(s) 1106, 1444, 1550, 2634, 3206
BsmI GAATGC 1 cut(s) 2322
BsnI GGCC 9 cut(s) 178, 712, 741, 1866, 2226, 2295, 2427, 2726, 3118
BsoBI CYCGRG 2 cut(s) 170, 184
Bsp1286I GDGCHC 3 cut(s) 891, 2715, 3326
Bsp1407I TGTACA 1 cut(s) 3291
Bsp19I CCATGG 2 cut(s) 2129, 2428
BspANI GGCC 9 cut(s) 178, 712, 741, 1866, 2226, 2295, 2427, 2726, 3118
BspFNI CGCG 1 cut(s) 3111
BspHI TCATGA 2 cut(s) 2239, 2926
BspLI GGNNCC 7 cut(s) 58, 497, 1460, 1956, 3030, 3160, 3428
BspMAI CTGCAG 3 cut(s) 808, 1285, 1455
BspMI ACCTGC 2 cut(s) 1234, 2983
BspQI GCTCTTC 2 cut(s) 255, 2539
BspT107I GGYRCC 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 958, 2042
BsrGI TGTACA 1 cut(s) 3291
BsrI ACTGG 3 cut(s) 606, 992, 1167
BssECI CCNNGG 7 cut(s) 1462, 1568, 1958, 2129, 2350, 2428, 2527
BssNI GRCGYC 1 cut(s) 345
BssSI CACGAG 3 cut(s) 2019, 3254, 3300
BssT1I CCWWGG 3 cut(s) 2129, 2350, 2428
Bst2BI CACGAG 3 cut(s) 2019, 3254, 3300
Bst2UI CCWGG 4 cut(s) 462, 1464, 1570, 1655
Bst4CI ACNGT 7 cut(s) 1501, 1897, 2192, 2864, 2884, 3197, 3533
BstACI GRCGYC 1 cut(s) 345
BstAPI GCANNNNNTGC 2 cut(s) 2024, 3106
BstAUI TGTACA 1 cut(s) 3291
BstC8I GCNNGC 2 cut(s) 1843, 2224
BstDSI CCRYGG 2 cut(s) 2129, 2428
BstFNI CGCG 1 cut(s) 3111
BstHHI GCGC 4 cut(s) 1231, 1932, 3111, 3448
BstMAI GTCTC 4 cut(s) 961, 1850, 2260, 2393
BstMCI CGRYCG 1 cut(s) 3115
BstNI CCWGG 4 cut(s) 462, 1464, 1570, 1655
BstNSI RCATGY 3 cut(s) 1915, 2105, 2378
BstPAI GACNNNNGTC 1 cut(s) 1245
BstSCI CCNGG 5 cut(s) 460, 627, 1462, 1568, 1653
BstSFI CTRYAG 5 cut(s) 804, 1257, 1281, 1451, 1500
BstUI CGCG 1 cut(s) 3111
BstV2I GAAGAC 4 cut(s) 445, 689, 2751, 2850
BstXI CCANNNNNNTGG 1 cut(s) 1654
Bsu36I CCTNAGG 1 cut(s) 3033
BsuI GTATCC 1 cut(s) 625
BsuRI GGCC 9 cut(s) 178, 712, 741, 1866, 2226, 2295, 2427, 2726, 3118
BtgI CCRYGG 2 cut(s) 2129, 2428
BtrI CACGTC 2 cut(s) 3182, 3521
BtsI GCAGTG 1 cut(s) 2217
BveI ACCTGC 2 cut(s) 1234, 2983
Cac8I GCNNGC 2 cut(s) 1843, 2224
CaiI CAGNNNCTG 2 cut(s) 1058, 2750
CciI TCATGA 2 cut(s) 2239, 2926
CfoI GCGC 4 cut(s) 1231, 1932, 3111, 3448
Cfr13I GGNCC 7 cut(s) 625, 710, 739, 2972, 3159, 3169, 3515
CseI GACGC 2 cut(s) 353, 2636
Csp6I GTAC 8 cut(s) 946, 1076, 1594, 2188, 2311, 2597, 2817, 3292
CviQI GTAC 8 cut(s) 946, 1076, 1594, 2188, 2311, 2597, 2817, 3292
DraI TTTAAA 1 cut(s) 1797
EaeI YGGCCR 2 cut(s) 1864, 2425
Ecl136II GAGCTC 1 cut(s) 889
Eco130I CCWWGG 3 cut(s) 2129, 2350, 2428
Eco147I AGGCCT 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 891
Eco32I GATATC 1 cut(s) 3527
Eco47I GGWCC 5 cut(s) 625, 2972, 3159, 3169, 3515
Eco53kI GAGCTC 1 cut(s) 889
Eco57I CTGAAG 2 cut(s) 266, 803
Eco81I CCTNAGG 1 cut(s) 3033
Eco88I CYCGRG 2 cut(s) 170, 184
EcoICRI GAGCTC 1 cut(s) 889
EcoO109I RGGNCCY 1 cut(s) 739
EcoRII CCWGG 4 cut(s) 460, 1462, 1568, 1653
EcoRV GATATC 1 cut(s) 3527
EcoT14I CCWWGG 3 cut(s) 2129, 2350, 2428
EcoT38I GRGCYC 1 cut(s) 891
ErhI CCWWGG 3 cut(s) 2129, 2350, 2428
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 5 cut(s) 1106, 1444, 1550, 2634, 3206
FauI CCCGC 1 cut(s) 261
FauNDI CATATG 3 cut(s) 913, 1088, 2340
FblI GTMKAC 1 cut(s) 1622
FriOI GRGCYC 1 cut(s) 891
FspI TGCGCA 1 cut(s) 3447
GlaI GCGC 4 cut(s) 1230, 1931, 3110, 3447
GsuI CTGGAG 4 cut(s) 975, 1035, 3243, 3570
HaeIII GGCC 9 cut(s) 178, 712, 741, 1866, 2226, 2295, 2427, 2726, 3118
HapII CCGG 4 cut(s) 48, 60, 628, 2287
HgaI GACGC 2 cut(s) 353, 2636
HhaI GCGC 4 cut(s) 1231, 1932, 3111, 3448
Hin1I GRCGYC 1 cut(s) 345
Hin6I GCGC 4 cut(s) 1229, 1930, 3109, 3446
HinP1I GCGC 4 cut(s) 1229, 1930, 3109, 3446
HincII GTYRAC 4 cut(s) 301, 1250, 1623, 2266
HindII GTYRAC 4 cut(s) 301, 1250, 1623, 2266
HpaII CCGG 4 cut(s) 48, 60, 628, 2287
HphI GGTGA 3 cut(s) 1394, 2147, 3212
Hpy99I CGWCG 2 cut(s) 364, 3525
HpyCH4III ACNGT 7 cut(s) 1501, 1897, 2192, 2864, 2884, 3197, 3533
HpyCH4IV ACGT 2 cut(s) 3181, 3520
HpySE526I ACGT 2 cut(s) 3181, 3520
Hsp92I GRCGYC 1 cut(s) 345
HspAI GCGC 4 cut(s) 1229, 1930, 3109, 3446
LguI GCTCTTC 2 cut(s) 255, 2539
LmnI GCTCC 7 cut(s) 62, 850, 1464, 1656, 2710, 3088, 3314
LweI GCATC 7 cut(s) 475, 1030, 1255, 1354, 1432, 2056, 2553
MaeII ACGT 2 cut(s) 3181, 3520
MfeI CAATTG 2 cut(s) 1493, 2114
MhlI GDGCHC 3 cut(s) 891, 2715, 3326
MlsI TGGCCA 1 cut(s) 2427
MluNI TGGCCA 1 cut(s) 2427
MlyI GAGTC 9 cut(s) 143, 176, 646, 1184, 1255, 2239, 2395, 2653, 3227
MmeI TCCRAC 6 cut(s) 342, 535, 1560, 1644, 1896, 3166
Mox20I TGGCCA 1 cut(s) 2427
MroXI GAANNNNTTC 4 cut(s) 452, 770, 816, 1066
MscI TGGCCA 1 cut(s) 2427
MslI CAYNNNNRTG 4 cut(s) 858, 2483, 2931, 3180
Msp20I TGGCCA 1 cut(s) 2427
MspA1I CMGCKG 5 cut(s) 268, 809, 1055, 1154, 1775
MspI CCGG 4 cut(s) 48, 60, 628, 2287
MspR9I CCNGG 5 cut(s) 462, 629, 1464, 1570, 1655
MunI CAATTG 2 cut(s) 1493, 2114
Mva1269I GAATGC 1 cut(s) 2322
MvaI CCWGG 4 cut(s) 462, 1464, 1570, 1655
MvnI CGCG 1 cut(s) 3111
NciI CCSGG 1 cut(s) 629
NcoI CCATGG 2 cut(s) 2129, 2428
NdeI CATATG 3 cut(s) 913, 1088, 2340
NlaIV GGNNCC 7 cut(s) 58, 497, 1460, 1956, 3030, 3160, 3428
NmeAIII GCCGAG 3 cut(s) 85, 652, 1939
NmuCI GTSAC 5 cut(s) 341, 1010, 1382, 1478, 2858
NsbI TGCGCA 1 cut(s) 3447
NspI RCATGY 3 cut(s) 1915, 2105, 2378
PagI TCATGA 2 cut(s) 2239, 2926
PceI AGGCCT 1 cut(s) 178
PciI ACATGT 2 cut(s) 1911, 2374
PciSI GCTCTTC 2 cut(s) 255, 2539
PctI GAATGC 1 cut(s) 2322
PdmI GAANNNNTTC 4 cut(s) 452, 770, 816, 1066
PflFI GACNNNGTC 1 cut(s) 3519
PflMI CCANNNNNTGG 3 cut(s) 707, 1007, 1058
PfoI TCCNGGA 1 cut(s) 460
PleI GAGTC 9 cut(s) 142, 176, 646, 1183, 1254, 2238, 2395, 2653, 3226
PpsI GAGTC 9 cut(s) 142, 176, 646, 1183, 1254, 2238, 2395, 2653, 3226
PscI ACATGT 2 cut(s) 1911, 2374
PshAI GACNNNNGTC 1 cut(s) 1245
PshBI ATTAAT 1 cut(s) 1925
Psp124BI GAGCTC 1 cut(s) 891
Psp6I CCWGG 4 cut(s) 460, 1462, 1568, 1653
PspGI CCWGG 4 cut(s) 460, 1462, 1568, 1653
PspN4I GGNNCC 7 cut(s) 58, 497, 1460, 1956, 3030, 3160, 3428
PspPI GGNCC 7 cut(s) 625, 710, 739, 2972, 3159, 3169, 3515
PstI CTGCAG 3 cut(s) 808, 1285, 1455
PstNI CAGNNNCTG 2 cut(s) 1058, 2750
PsyI GACNNNGTC 1 cut(s) 3519
PvuII CAGCTG 4 cut(s) 809, 1055, 1154, 1775
RsaI GTAC 8 cut(s) 947, 1077, 1595, 2189, 2312, 2598, 2818, 3293
RsaNI GTAC 8 cut(s) 946, 1076, 1594, 2188, 2311, 2597, 2817, 3292
RseI CAYNNNNRTG 4 cut(s) 858, 2483, 2931, 3180
SacI GAGCTC 1 cut(s) 891
SalI GTCGAC 1 cut(s) 1621
SapI GCTCTTC 2 cut(s) 255, 2539
Sau96I GGNCC 7 cut(s) 625, 710, 739, 2972, 3159, 3169, 3515
SchI GAGTC 9 cut(s) 143, 176, 646, 1184, 1255, 2239, 2395, 2653, 3227
ScrFI CCNGG 5 cut(s) 462, 629, 1464, 1570, 1655
SduI GDGCHC 3 cut(s) 891, 2715, 3326
SfaNI GCATC 7 cut(s) 475, 1030, 1255, 1354, 1432, 2056, 2553
SfcI CTRYAG 5 cut(s) 804, 1257, 1281, 1451, 1500
SinI GGWCC 5 cut(s) 625, 2972, 3159, 3169, 3515
SmiMI CAYNNNNRTG 4 cut(s) 858, 2483, 2931, 3180
SmlI CTYRAG 6 cut(s) 110, 289, 1853, 2096, 2705, 3062
SmoI CTYRAG 6 cut(s) 110, 289, 1853, 2096, 2705, 3062
SseBI AGGCCT 1 cut(s) 178
SspI AATATT 4 cut(s) 589, 1732, 1831, 1923
SstI GAGCTC 1 cut(s) 891
StuI AGGCCT 1 cut(s) 178
StyD4I CCNGG 5 cut(s) 460, 627, 1462, 1568, 1653
StyI CCWWGG 3 cut(s) 2129, 2350, 2428
TaaI ACNGT 7 cut(s) 1501, 1897, 2192, 2864, 2884, 3197, 3533
TaiI ACGT 2 cut(s) 3184, 3523
TaqI TCGA 6 cut(s) 132, 1425, 1622, 2502, 2662, 3523
TaqII GACCGA 1 cut(s) 2541
TatI WGTACW 3 cut(s) 1075, 2596, 3291
TauI GCSGC 1 cut(s) 3344
TseFI GTSAC 5 cut(s) 341, 1010, 1382, 1478, 2858
Tsp45I GTSAC 5 cut(s) 341, 1010, 1382, 1478, 2858
TspGWI ACGGA 3 cut(s) 140, 2024, 2328
Tth111I GACNNNGTC 1 cut(s) 3519
Van91I CCANNNNNTGG 3 cut(s) 707, 1007, 1058
VpaK11BI GGWCC 5 cut(s) 625, 2972, 3159, 3169, 3515
VspI ATTAAT 1 cut(s) 1925
XapI RAATTY 6 cut(s) 311, 646, 1435, 1519, 1948, 2246
XbaI TCTAGA 1 cut(s) 1807
XceI RCATGY 3 cut(s) 1915, 2105, 2378
XcmI CCANNNNNNNNNTGG 1 cut(s) 1576
XmaJI CCTAGG 1 cut(s) 2350
XmiI GTMKAC 1 cut(s) 1622
XmnI GAANNNNTTC 4 cut(s) 452, 770, 816, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.