RchiOBHm_Chr2g0128851

Sodium/calcium exchanger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015661
Physical Location & Seq
Forward (+)
44195654 .. 44199307
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1743 bp
ATGGCAAAGAAAATTGCGTTATTCCTTTATTTCCTATCCATCATGCTAGTTAATCTAACCAGCTTTGGCCAAAGTCGTTCTATTGCTACAAGTGACTTCAATTTAGTGTCTGATGGAGTAAACCACGCTACTGGGGATAAACCCAATTTTCTTTGGTTAAAGCATAGCTTGTTTGCATCCGATAGCTGTGATGAGACATATGGGTTCTTGCCATGCACCAACACAGTCCTTGGTAATATCTTCCTCATTATCGTCTATGGTTACTTGATGTTCCTTTCAGCCAAGTTATTGTCAGCTGGAAGTGAGATTCTGCTGCAAATTTTAGGACCTGGTATCATAGGGGGTCTCTTCTTGCCTCTGTTGAGTGCTGTACCTGATGCCACCATTATCCTTGCATCAGGACTTTCTGGGGACACGGCAACGGCACAAAGTCAGGTCTCCGTTGGAATGGGCTTGCTAGCTGGATCCAATGTAATGCTTCTCACAATCCTATGGGGGACCTGTCTTCTAGTTGGCAAATGTGACCTTGAAAATTCAGTTGCAGTTGATGAAAAAGATACAAAGCGTTTTAGCTTAACTGGATCTGGATCTGGAGTGAGCACAGATATTTGGACAAGCTATGCTGCATGGATCATGGTTATCTCCATTATTCCTTTTGTAATAGTCCAACTACCTCAAGTTTTCACTGCGACTTTGGATAGTAGCTTGCCAATTCTAAGTTCACTCATTGTTGCAATCTGTTTTGTGATCGCTTATAGCATCTATCAGATTTTTCAGCCTTGGATTCAGAAGAGAAGACTTGCATATGCAAAGCATAAGCATGTGATGTCAGAGATTCTAAAACAGTTGAAAACAAACGCGCTTGGAAAATTGCTTACAGATGATGGTGATCCTAACAAAGTTGTTATAGAGAAGTTATTCAGGACACTTGATCAGAACTCGGATGGTTATTTGAACACCACAGACTTAAGATCTTTGATAATAGGAATCCAATTTGATGATGACGTAGATATAAATATCGATGAAGCCATTTCTCAAGTGATGAGAGACTTTGACACAACTCGTGATTCGAAGATTGATGTTGATGAATTCTTCAAAGGAATTTCAAGGTGGCTTAACAAGGCTAAGCGTGCTGCAATGATGGACCGTGGAAAGGCTCCGCTATCAATGAAACTGTTAGATGATTTTGATAAGCAAACAAAGGAAGAACAAGATCGGTATGGGGATCAGATTGATGAGATTGTGGAGGATGTTAAAAATGCAAACTGGCATGCCTCAAAAGCAGTACTGATGTTGCTTCTGGGAACCATTGTTGCTGCTGTGGTTGCTGATCCCCTTGTTGATGCTGTTGACAACTTCTCAACAGCTACAAGTATTCCTACATTCTTCGTATCATTTGTTATTCTGCCTTTTGTTAGCTCCAGTGAGATTGTGTCCACTCTAATTTTTGTCAGCCGGAAGAAGCAGAGAACAGCATCATTGGCCTACTCTGCGATATATGGATCAGTAACAATGAGTAACATCCTCTCCCTGGCAGTGTTCTTGGCTCTTGTATACATTCGAAATCTGACATGGAGCTTCTCGTCTGAAGTTCTTGTCATTATAATTGTCTGCATTCTAATGGGAGTGGTTGCTGGTTTCCGCACCACCTTCCCTGTTTGGATGTCTTTTGGGGCTGTAGCGCTCTATCCACTTTCTCTGTTGCTGGTTTACATTCTTGAATATGTTTTTGGGTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

580

Amino Acids

63.75

Weight (kDa)

5.13

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 80 - 259 5.4e-07 Sodium/calcium exchanger protein
EF-hand_7 PF13499 302 - 368 3.9e-11 EF-hand domain pair
Na_Ca_ex PF01699 428 - 571 3.1e-11 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015661)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26990 FvH4_6g26990 FvH4_6g26990 FvH4_6g26990
malus_domestica MD17G1233800.v1.1
prunus_persica Prupe.3G125200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0128851
rosa_laevigata RLG00000019033
rosa_multiflora Rmu_sc0001643.1_g000033
rosa_roxburghii Rroxscaffold_2G00115170
rosa_rugosa Rorug02G0281800 Rorug02G0281800
rosa_samantha Rh2AG334300 Rh2CG321200 Rh2DG359000
rosa_wichuraiana Rw2G027040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1604
AccI GTMKAC 1 cut(s) 1554
AccII CGCG 1 cut(s) 860
AciI CCGC 2 cut(s) 1160, 1642
AclWI GGATC 9 cut(s) 459, 472, 589, 595, 638, 884, 1233, 1325, 1510
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 4 cut(s) 318, 532, 1088, 1101
AcuI CTGAAG 1 cut(s) 1608
AfaI GTAC 2 cut(s) 372, 1287
AfeI AGCGCT 1 cut(s) 1683
AfiI CCNNNNNNNGG 2 cut(s) 1153, 1531
AflII CTTAAG 1 cut(s) 967
AgsI TTSAA 7 cut(s) 100, 530, 850, 955, 1096, 1107, 1721
AjnI CCWGG 2 cut(s) 328, 1530
AjuI GAANNNNNNNTTGG 3 cut(s) 703, 735, 1713
Alw21I GWGCWC 1 cut(s) 602
Alw26I GTCTC 4 cut(s) 188, 350, 442, 1041
AlwI GGATC 9 cut(s) 459, 472, 589, 595, 638, 884, 1233, 1325, 1510
Aor51HI AGCGCT 1 cut(s) 1683
AoxI GGCC 2 cut(s) 67, 1482
ApeKI GCWGC 4 cut(s) 313, 623, 1133, 1316
ApoI RAATTY 4 cut(s) 318, 532, 1088, 1101
Asp700I GAANNNNTTC 1 cut(s) 917
AspLEI GCGC 2 cut(s) 862, 1684
AspS9I GGNCC 3 cut(s) 326, 498, 1144
AsuHPI GGTGA 1 cut(s) 899
AsuII TTCGAA 2 cut(s) 1070, 1561
AsuNHI GCTAGC 1 cut(s) 457
AvaII GGWCC 3 cut(s) 326, 498, 1144
BalI TGGCCA 1 cut(s) 69
BamHI GGATCC 1 cut(s) 464
BauI CACGAG 1 cut(s) 1062
BbsI GAAGAC 2 cut(s) 497, 802
Bbv12I GWGCWC 1 cut(s) 602
BbvI GCAGC 4 cut(s) 300, 610, 1120, 1303
BccI CCATC 5 cut(s) 47, 107, 878, 938, 1135
BceAI ACGGC 2 cut(s) 432, 438
BciT130I CCWGG 2 cut(s) 330, 1532
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 4 cut(s) 188, 350, 442, 1041
BfaI CTAG 3 cut(s) 47, 458, 509
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 1 cut(s) 1685
BfrI CTTAAG 1 cut(s) 967
BglII AGATCT 1 cut(s) 971
BisI GCNGC 4 cut(s) 314, 624, 1134, 1317
BlpI GCTNAGC 1 cut(s) 1125
BlsI GCNGC 4 cut(s) 315, 625, 1135, 1318
BmcAI AGTACT 1 cut(s) 1287
Bme1390I CCNGG 2 cut(s) 330, 1532
Bme18I GGWCC 3 cut(s) 326, 498, 1144
BmgT120I GGNCC 3 cut(s) 326, 498, 1144
BmiI GGNNCC 4 cut(s) 466, 499, 1158, 1306
BmrFI CCNGG 2 cut(s) 330, 1532
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 6 cut(s) 185, 367, 404, 768, 1333, 1484
BmtI GCTAGC 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 497, 802
BpmI CTGGAG 2 cut(s) 612, 1405
Bpu1102I GCTNAGC 1 cut(s) 1125
Bpu14I TTCGAA 2 cut(s) 1070, 1561
BpuEI CTTGAG 2 cut(s) 660, 1020
Bsa29I ATCGAT 1 cut(s) 1020
BsaBI GATNNNNATC 2 cut(s) 586, 888
BsaI GGTCTC 2 cut(s) 350, 442
BsaJI CCNNGG 4 cut(s) 229, 779, 1147, 1530
BsaXI ACNNNNNCTCC 2 cut(s) 1511, 1541
Bsc4I CCNNNNNNNGG 2 cut(s) 1153, 1531
Bse1I ACTGG 4 cut(s) 136, 583, 1271, 1422
Bse3DI GCAATG 1 cut(s) 1143
Bse8I GATNNNNATC 2 cut(s) 586, 888
BseBI CCWGG 2 cut(s) 330, 1532
BseCI ATCGAT 1 cut(s) 1020
BseDI CCNNGG 4 cut(s) 229, 779, 1147, 1530
BseGI GGATG 5 cut(s) 176, 949, 1255, 1521, 1668
BseJI GATNNNNATC 2 cut(s) 586, 888
BseLI CCNNNNNNNGG 2 cut(s) 1153, 1531
BseMI GCAATG 1 cut(s) 1143
BseNI ACTGG 4 cut(s) 136, 583, 1271, 1422
BseXI GCAGC 4 cut(s) 300, 610, 1120, 1303
Bsh1236I CGCG 1 cut(s) 860
BshFI GGCC 2 cut(s) 69, 1484
BshVI ATCGAT 1 cut(s) 1020
BsiHKAI GWGCWC 1 cut(s) 602
BsiSI CCGG 1 cut(s) 1456
BslFI GGGAC 2 cut(s) 425, 511
BslI CCNNNNNNNGG 2 cut(s) 1153, 1531
BsmAI GTCTC 4 cut(s) 188, 350, 442, 1041
BsmFI GGGAC 2 cut(s) 425, 511
BsmI GAATGC 1 cut(s) 1614
BsnI GGCC 2 cut(s) 69, 1484
Bso31I GGTCTC 2 cut(s) 350, 442
Bsp119I TTCGAA 2 cut(s) 1070, 1561
Bsp1286I GDGCHC 1 cut(s) 602
Bsp1720I GCTNAGC 1 cut(s) 1125
BspACI CCGC 2 cut(s) 1160, 1642
BspANI GGCC 2 cut(s) 69, 1484
BspDI ATCGAT 1 cut(s) 1020
BspFNI CGCG 1 cut(s) 860
BspLI GGNNCC 4 cut(s) 466, 499, 1158, 1306
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 9 cut(s) 459, 472, 589, 595, 638, 884, 1233, 1325, 1510
BspT104I TTCGAA 2 cut(s) 1070, 1561
BspTI CTTAAG 1 cut(s) 967
BspTNI GGTCTC 2 cut(s) 350, 442
BsrDI GCAATG 1 cut(s) 1143
BsrI ACTGG 4 cut(s) 136, 583, 1271, 1422
BssECI CCNNGG 4 cut(s) 229, 779, 1147, 1530
BssNAI GTATAC 1 cut(s) 1555
BssSI CACGAG 1 cut(s) 1062
BssT1I CCWWGG 2 cut(s) 229, 779
Bst1107I GTATAC 1 cut(s) 1555
Bst2BI CACGAG 1 cut(s) 1062
Bst2UI CCWGG 2 cut(s) 330, 1532
Bst4CI ACNGT 4 cut(s) 226, 846, 1148, 1176
Bst6I CTCTTC 2 cut(s) 353, 785
BstAFI CTTAAG 1 cut(s) 967
BstBI TTCGAA 2 cut(s) 1070, 1561
BstC8I GCNNGC 5 cut(s) 455, 459, 707, 1131, 1272
BstDEI CTNAG 2 cut(s) 716, 1125
BstDSI CCRYGG 1 cut(s) 1147
BstF5I GGATG 5 cut(s) 176, 949, 1255, 1521, 1668
BstFNI CGCG 1 cut(s) 860
BstH2I RGCGCY 1 cut(s) 1685
BstHHI GCGC 2 cut(s) 862, 1684
BstMAI GTCTC 4 cut(s) 188, 350, 442, 1041
BstMWI GCNNNNNNNGC 5 cut(s) 1130, 1280, 1325, 1481, 1490
BstNI CCWGG 2 cut(s) 330, 1532
BstNSI RCATGY 2 cut(s) 824, 1274
BstSCI CCNGG 2 cut(s) 328, 1530
BstSFI CTRYAG 1 cut(s) 1677
BstUI CGCG 1 cut(s) 860
BstV1I GCAGC 4 cut(s) 300, 610, 1120, 1303
BstV2I GAAGAC 2 cut(s) 497, 802
BstX2I RGATCY 4 cut(s) 464, 581, 587, 971
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 4 cut(s) 464, 581, 587, 971
BstZ17I GTATAC 1 cut(s) 1555
Bsu15I ATCGAT 1 cut(s) 1020
BsuRI GGCC 2 cut(s) 69, 1484
BsuTUI ATCGAT 1 cut(s) 1020
BtgI CCRYGG 1 cut(s) 1147
BtsCI GGATG 5 cut(s) 176, 949, 1255, 1521, 1668
BtsI GCAGTG 2 cut(s) 684, 1542
BtsIMutI CAGTG 3 cut(s) 684, 1429, 1542
Cac8I GCNNGC 5 cut(s) 455, 459, 707, 1131, 1272
CfoI GCGC 2 cut(s) 862, 1684
Cfr13I GGNCC 3 cut(s) 326, 498, 1144
ClaI ATCGAT 1 cut(s) 1020
CsiI ACCWGGT 1 cut(s) 328
Csp6I GTAC 2 cut(s) 371, 1286
CviAII CATG 7 cut(s) 43, 213, 627, 634, 821, 1271, 1572
CviQI GTAC 2 cut(s) 371, 1286
DdeI CTNAG 2 cut(s) 716, 1125
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 353, 785
EarI CTCTTC 2 cut(s) 353, 785
Eco130I CCWWGG 2 cut(s) 229, 779
Eco31I GGTCTC 2 cut(s) 350, 442
Eco47I GGWCC 3 cut(s) 326, 498, 1144
Eco47III AGCGCT 1 cut(s) 1683
Eco57I CTGAAG 1 cut(s) 1608
EcoO109I RGGNCCY 2 cut(s) 326, 498
EcoRI GAATTC 1 cut(s) 1088
EcoRII CCWGG 2 cut(s) 328, 1530
EcoT14I CCWWGG 2 cut(s) 229, 779
ErhI CCWWGG 2 cut(s) 229, 779
FaeI CATG 7 cut(s) 46, 216, 630, 637, 824, 1274, 1575
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 2 cut(s) 425, 511
FatI CATG 7 cut(s) 42, 212, 626, 633, 820, 1270, 1571
FauNDI CATATG 2 cut(s) 199, 805
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 1 cut(s) 1554
Fnu4HI GCNGC 4 cut(s) 314, 624, 1134, 1317
FokI GGATG 5 cut(s) 163, 956, 1262, 1508, 1675
Fsp4HI GCNGC 4 cut(s) 314, 624, 1134, 1317
FspBI CTAG 3 cut(s) 47, 458, 509
GlaI GCGC 2 cut(s) 861, 1683
GluI GCNGC 4 cut(s) 314, 624, 1134, 1317
GsuI CTGGAG 2 cut(s) 612, 1405
HaeII RGCGCY 1 cut(s) 1685
HaeIII GGCC 2 cut(s) 69, 1484
HapII CCGG 1 cut(s) 1456
HhaI GCGC 2 cut(s) 862, 1684
Hin1II CATG 7 cut(s) 46, 216, 630, 637, 824, 1274, 1575
Hin6I GCGC 2 cut(s) 860, 1682
HinP1I GCGC 2 cut(s) 860, 1682
HincII GTYRAC 1 cut(s) 1351
HindII GTYRAC 1 cut(s) 1351
HinfI GANTC 5 cut(s) 307, 784, 835, 987, 1067
HpaII CCGG 1 cut(s) 1456
HphI GGTGA 1 cut(s) 899
Hpy166II GTNNAC 6 cut(s) 121, 722, 1351, 1437, 1555, 1711
Hpy188III TCNNGA 6 cut(s) 399, 585, 591, 922, 1064, 1718
Hpy8I GTNNAC 6 cut(s) 121, 722, 1351, 1437, 1555, 1711
HpyAV CCTTC 1 cut(s) 1660
HpyCH4III ACNGT 4 cut(s) 226, 846, 1148, 1176
HpyCH4IV ACGT 1 cut(s) 1005
HpyF10VI GCNNNNNNNGC 5 cut(s) 1130, 1280, 1325, 1481, 1490
HpyF3I CTNAG 2 cut(s) 716, 1125
HpySE526I ACGT 1 cut(s) 1005
Hsp92II CATG 7 cut(s) 46, 216, 630, 637, 824, 1274, 1575
HspAI GCGC 2 cut(s) 860, 1682
Ksp22I TGATCA 1 cut(s) 931
LmnI GCTCC 3 cut(s) 1162, 1424, 1575
Lsp1109I GCAGC 4 cut(s) 300, 610, 1120, 1303
LweI GCATC 6 cut(s) 185, 367, 404, 768, 1333, 1484
MabI ACCWGGT 1 cut(s) 328
MaeI CTAG 3 cut(s) 47, 458, 509
MaeII ACGT 1 cut(s) 1005
MaeIII GTNAC 5 cut(s) 92, 260, 521, 1507, 1517
MflI RGATCY 4 cut(s) 464, 581, 587, 971
MhlI GDGCHC 1 cut(s) 602
MlsI TGGCCA 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 69
MmeI TCCRAC 2 cut(s) 424, 691
MnlI CCTC 6 cut(s) 254, 366, 684, 1240, 1285, 1535
Mox20I TGGCCA 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 917
MscI TGGCCA 1 cut(s) 69
MseI TTAA 6 cut(s) 51, 158, 575, 968, 1116, 1254
MslI CAYNNNNRTG 2 cut(s) 819, 1619
Msp20I TGGCCA 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 296
MspCI CTTAAG 1 cut(s) 967
MspI CCGG 1 cut(s) 1456
MspR9I CCNGG 2 cut(s) 330, 1532
Mva1269I GAATGC 1 cut(s) 1614
MvaI CCWGG 2 cut(s) 330, 1532
MvnI CGCG 1 cut(s) 860
MwoI GCNNNNNNNGC 5 cut(s) 1130, 1280, 1325, 1481, 1490
NdeI CATATG 2 cut(s) 199, 805
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 7 cut(s) 46, 216, 630, 637, 824, 1274, 1575
NlaIV GGNNCC 4 cut(s) 466, 499, 1158, 1306
NmuCI GTSAC 2 cut(s) 92, 521
NspI RCATGY 2 cut(s) 824, 1274
NspV TTCGAA 2 cut(s) 1070, 1561
PaeI GCATGC 1 cut(s) 1274
PctI GAATGC 1 cut(s) 1614
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 5 cut(s) 307, 784, 835, 987, 1067
PkrI GCNGC 4 cut(s) 315, 625, 1135, 1318
PpuMI RGGWCCY 2 cut(s) 326, 498
PsiI TTATAA 1 cut(s) 1604
Psp5II RGGWCCY 2 cut(s) 326, 498
Psp6I CCWGG 2 cut(s) 328, 1530
PspGI CCWGG 2 cut(s) 328, 1530
PspN4I GGNNCC 4 cut(s) 466, 499, 1158, 1306
PspPI GGNCC 3 cut(s) 326, 498, 1144
PspPPI RGGWCCY 2 cut(s) 326, 498
PsuI RGATCY 4 cut(s) 464, 581, 587, 971
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 2 cut(s) 372, 1287
RsaNI GTAC 2 cut(s) 371, 1286
RseI CAYNNNNRTG 2 cut(s) 819, 1619
SaqAI TTAA 6 cut(s) 51, 158, 575, 968, 1116, 1254
SatI GCNGC 4 cut(s) 314, 624, 1134, 1317
Sau96I GGNCC 3 cut(s) 326, 498, 1144
ScaI AGTACT 1 cut(s) 1287
ScrFI CCNGG 2 cut(s) 330, 1532
SduI GDGCHC 1 cut(s) 602
SexAI ACCWGGT 1 cut(s) 328
SfaNI GCATC 6 cut(s) 185, 367, 404, 768, 1333, 1484
SfcI CTRYAG 1 cut(s) 1677
SfuI TTCGAA 2 cut(s) 1070, 1561
SinI GGWCC 3 cut(s) 326, 498, 1144
SmiMI CAYNNNNRTG 2 cut(s) 819, 1619
SmlI CTYRAG 3 cut(s) 675, 967, 1035
SmoI CTYRAG 3 cut(s) 675, 967, 1035
SphI GCATGC 1 cut(s) 1274
SsiI CCGC 2 cut(s) 1160, 1642
SspMI CTAG 3 cut(s) 47, 458, 509
StyD4I CCNGG 2 cut(s) 328, 1530
StyI CCWWGG 2 cut(s) 229, 779
TaaI ACNGT 4 cut(s) 226, 846, 1148, 1176
TaiI ACGT 1 cut(s) 1008
TaqI TCGA 3 cut(s) 1020, 1070, 1561
TatI WGTACW 1 cut(s) 1285
TfiI GAWTC 5 cut(s) 307, 784, 835, 987, 1067
Tru1I TTAA 6 cut(s) 51, 158, 575, 968, 1116, 1254
Tru9I TTAA 6 cut(s) 51, 158, 575, 968, 1116, 1254
TscAI CASTG 3 cut(s) 691, 1429, 1542
TseFI GTSAC 2 cut(s) 92, 521
TseI GCWGC 4 cut(s) 313, 623, 1133, 1316
Tsp45I GTSAC 2 cut(s) 92, 521
TspDTI ATGAA 4 cut(s) 564, 1038, 1101, 1184
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 3 cut(s) 691, 1429, 1542
Vha464I CTTAAG 1 cut(s) 967
VpaK11BI GGWCC 3 cut(s) 326, 498, 1144
XapI RAATTY 4 cut(s) 318, 532, 1088, 1101
XceI RCATGY 2 cut(s) 824, 1274
XmiI GTMKAC 1 cut(s) 1554
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 3 cut(s) 47, 458, 509
ZrmI AGTACT 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.