RchiOBHm_Chr2g0130091

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011362
Physical Location & Seq
Forward (+)
46016127 .. 46022815
6689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2607 bp
ATGGCTTTGGTCGATGTCACCAAATCTTGCCTCGATTCAATTAGCCAGATATCGGAGCACATTGAAGGTTCTGTTCTTTACCTAGATGCTGGATCTACAGAGAGTTTCCAGTACATTGGAGCGTTTCCTCTCTTACTCAACCATGGAGTTCGTGCCATTTGCAGTTTGGAAAATATGTCTTCTCTTGATGCGGTAGTTGATTGGAATGCAAATTCTGATCCTGATAGGAAAGTCGTGGTGGTAACAACTCGTTTGCTAAGTGATGCACATCGATATATATTACGTTGTCTTAGCACTCATCCAGCTGTCCATTGTTGTACTATATTCACATCTATCTCAGAGATAGCACATTCAGCATATCCTGATTCACCTCTGGGACCAGATGCATTTCATGAATATGAATCCTTGCTTGTCCAAGATTATGAGGAACTTGTCAAGAAAGGTGTAAAGAAACCCTTACAGCCTGGTGTTAGCAACTTCAAGGATAATATAGATTTAGGAGATGAAGGACGGTCACAACTTGCTCCGAGTGAAGAGGACATATCTCACCCCCAAGCTAGTTCAACTGCAAGAGATTCCTATGGAGAGAATCTGATTGCTGATACAGAAGAAGTAGGGCAAAATTTGGTTGTTTCTGTGCGTCACTTCCCAATGATTATCTGTCCTTTTTCACCAAGAGTCTTTGTTTTACCTCCAGAGGGATCAGTTGCTGAAGCATACTTATCAGCTAAACATGAGGATGCCCTTAGTCCTGGATTACCCTCCTTAAGTACTGGGTTGCTTTCTGATGGTGATGATATTCCTCCTGGGGCAACTCTTACAGCAAATTTTCTTTACCATTTTGCGGCCAAGTTGGACCTGAAGATGGAGATATTTTCACTTGGTGATCTCTCAAAAACTGTGGGGAAGATGTTTACAGACATGTCAAGTCTTTATGATGTTGGTCGCCGTAAACGATCAGCAGGGCTATTACTCATTGACCGTACACTAGATCTTCTTACTCCATGCTGTCATGGAGATTCACTTGTTGACCGTGTGTTTTCATCCTTGCCTCGTAGGGAAAATACAGCATCCTATACTCAAATAAAAACCTCAAAAAGCCAACTAAAACAGGGCCCTTCAAACCTGGAACGTGCTTCTCTTGATGTTCAGATACCACTTGCTAAAATTCTAAGTGAAGAAGATTGCAAGATAGAGAACTTTCGGCTATTAGAAAGTATTGAAGCTTTTCTATGTGGGTGGGATTCTGGAAACTCTGCTTCTCAAGTTTTAGATCTGAGCAATCTCAAGAACAAAATTCATGATGAGAAGCTTCCCCAGTTAGAGAATGAGCTTCTAAGAGGGTCTCTTGTGTCCACTGAAAGTTTTCGAGGAACACCATATTTGGAAACTCTACTAGATAGGAAAACCAAAGATGGGACCATACTAGTAAAGAAGTGGCTTCAAGAAGCTCTTCGTCGGGAAAATATCTCCGTGAATGTGAAATCCCGTCCTGGTTTTGCTACAAAGTCAGAGTTGCAAGCTATGACAAAAGCACTGGCCAAATCCCAGTCATCTCTACTGAAAAACAAAGGAATTATTCAGTTGGCAGCGGCTGTAGTGGCTGCACTTGATGAATCTCTTTCATCCAGATGGGAAGCATTTAGCAATGCAGAGAAAATATTAAGTGTAAGTGCTGAAGACACAAGCCAGAGTCTTGCTGTGCAAATTGGTGATTTTATTAATAAGAGTGCTTTGTTGGGATTGCATGGACAGAAGAATGGAAAATTTGGGGTCTCCCAAGGGGTACTTTCTTTTCAGGATACTTTGTATCTTATGATTTCTGGATATATATTAGCTGGTGAGAATTTCCCAACAGCTGGGAATGATGGCCCCTTTTCTTGGCAAGAGGAGCAACTATTGAAAGATTCTATTGTTGAAGCAATTCTTGAAAACCCTTCAATAGCAAAACTGAAGTTTCTTCATGGCCTAATGGAAGAGCTTGAGACCAACTTAAGCAGGATCAAATCAGAGGAAAGCAAGGGGACATCCTCAGATCAAATAGATATTGATGATCTTGATGACGATCAGTGGGGTAAATGGGGTGATGAAGATGTTGATGATACAAATAACAGTAAAGAGAAAGTGTATGATGACATGCAGCTGAAGTTGGAGTTGCGTGATAGGGTCGATAACCTTTTCAAATTCCTTCACAAGTTATCCAGTTTGAAGAGTCGAAACATACCATTGAAGGATGGGGCTTTAGATTCGGACAATAATTTCAGTGGAGATCCATATGCTAGTAGAGGATTACTTTATAAGCTTCTAACTCGGGTATTGAGCAAGAATGATGTTCCTGGTTTGGAGTATCACTCTTCTACTGTGGGGCAACTATTTAAAAGTGGCTTCAGAAGATTTGGCCTTGGGCAGGCAAAACCAAGTTTAGCCGATCAAAATGTCATCCTTGTATTTGTTATTGGGGGCATTAATGGTGTGGAGGTTCGTGAAGCTCAAGAGGCATTATCTGAAAGTGGAAGACCGGATATAGAAATGATTCTTGGTGGAACAACTCTCTTAACTCCTGACGACATGCTTGACTTGCTACTGGGGAAATCTAGTTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006508 GO:0006624 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016485 GO:0019538 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec1

Protein Analysis

868

Amino Acids

95.39

Weight (kDa)

4.97

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 496 - 859 8.9e-09 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42700 AT2G42700 AT2G42700
fragaria_vesca FvH4_6g27600 FvH4_6g27600
malus_domestica MD11G1004500.v1.1
prunus_persica Prupe.3G115700_v2.0.a1
pyrus_communis pycom09g15450
rosa_chinensis RchiOBHm_Chr2g0130091
rosa_laevigata RLG00000019126
rosa_multiflora Rmu_co7958197.1_g000001 Rmu_co8394299.1_g000001 Rmu_sc0010460.1_g000011
rosa_roxburghii Rroxscaffold_2G00114090
rosa_rugosa Rorug02G0291000
rosa_samantha Rh2AG342800 Rh2BG350500 Rh2CG329900 Rh2DG369000
rosa_wichuraiana Rw2G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2298
AccB7I CCANNNNNTGG 1 cut(s) 1859
AciI CCGC 3 cut(s) 191, 845, 1592
AclWI GGATC 5 cut(s) 100, 212, 709, 2009, 2264
AcoI YGGCCR 2 cut(s) 846, 1539
AcsI RAATTY 8 cut(s) 211, 622, 826, 1167, 1296, 1766, 1846, 2183
AcuI CTGAAG 6 cut(s) 732, 881, 1698, 1973, 2165, 2371
AdeI CACNNNGTG 1 cut(s) 884
AfaI GTAC 5 cut(s) 113, 319, 772, 985, 1788
AfiI CCNNNNNNNGG 8 cut(s) 52, 698, 844, 865, 1416, 1859, 1881, 2407
AflII CTTAAG 2 cut(s) 766, 1993
AflIII ACRYGT 1 cut(s) 921
AhlI ACTAGT 1 cut(s) 1426
AjnI CCWGG 6 cut(s) 463, 751, 805, 1125, 1492, 2335
AjuI GAANNNNNNNTTGG 2 cut(s) 2520, 2552
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 3 cut(s) 1350, 1780, 1979
AlwI GGATC 5 cut(s) 100, 212, 709, 2009, 2264
AlwNI CAGNNNCTG 2 cut(s) 710, 1595
Ama87I CYCGRG 1 cut(s) 2310
AoxI GGCC 6 cut(s) 846, 1114, 1539, 1870, 1966, 2398
ApaI GGGCCC 1 cut(s) 1118
ApeKI GCWGC 3 cut(s) 1589, 1604, 2140
ApoI RAATTY 8 cut(s) 211, 622, 826, 1167, 1296, 1766, 1846, 2183
AseI ATTAAT 2 cut(s) 1722, 2466
Asp700I GAANNNNTTC 5 cut(s) 1227, 1365, 1452, 1923, 2532
AspS9I GGNCC 6 cut(s) 377, 856, 1114, 1115, 1419, 1871
AsuHPI GGTGA 9 cut(s) 10, 360, 539, 663, 803, 896, 1724, 1853, 2096
AvaI CYCGRG 1 cut(s) 2310
AvaII GGWCC 3 cut(s) 377, 856, 1419
BaeGI GKGCMC 1 cut(s) 1118
BalI TGGCCA 1 cut(s) 1541
BanII GRGCYC 1 cut(s) 1118
BbsI GAAGAC 3 cut(s) 171, 1686, 2521
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 1591, 1601, 2152
BccI CCATC 6 cut(s) 782, 859, 1409, 1626, 1862, 2228
BceAI ACGGC 1 cut(s) 933
BciT130I CCWGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
BciVI GTATCC 1 cut(s) 1795
BcoDI GTCTC 3 cut(s) 1350, 1780, 1979
BcuI ACTAGT 1 cut(s) 1426
BfaI CTAG 7 cut(s) 83, 558, 989, 1397, 1427, 2280, 2595
BfmI CTRYAG 2 cut(s) 96, 1596
BfrI CTTAAG 2 cut(s) 766, 1993
BfuI GTATCC 1 cut(s) 1795
BglII AGATCT 2 cut(s) 991, 1273
BisI GCNGC 5 cut(s) 846, 1590, 1593, 1605, 2141
BlsI GCNGC 5 cut(s) 847, 1591, 1594, 1606, 2142
BmcAI AGTACT 1 cut(s) 772
Bme1390I CCNGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
Bme18I GGWCC 3 cut(s) 377, 856, 1419
BmeT110I CYCGRG 1 cut(s) 2310
BmgT120I GGNCC 6 cut(s) 377, 856, 1114, 1115, 1419, 1871
BmiI GGNNCC 4 cut(s) 378, 1116, 1420, 1873
BmrFI CCNGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
BmrI ACTGGG 4 cut(s) 783, 1312, 1543, 2594
BmsI GCATC 6 cut(s) 76, 178, 253, 373, 730, 1079
BmuI ACTGGG 4 cut(s) 783, 1312, 1543, 2594
BpiI GAAGAC 3 cut(s) 171, 1686, 2521
BplI GAGNNNNNCTC 2 cut(s) 2336, 2368
BpmI CTGGAG 1 cut(s) 678
BpuEI CTTGAG 4 cut(s) 1248, 1271, 2003, 2475
Bsa29I ATCGAT 1 cut(s) 271
BsaBI GATNNNNATC 2 cut(s) 267, 2064
BsaI GGTCTC 3 cut(s) 1350, 1780, 1979
BsaJI CCNNGG 4 cut(s) 142, 806, 1780, 2401
BsaWI WCCGGW 1 cut(s) 2518
Bsc4I CCNNNNNNNGG 8 cut(s) 52, 698, 844, 865, 1416, 1859, 1881, 2407
Bse1I ACTGG 7 cut(s) 109, 778, 1318, 1542, 1549, 2202, 2589
Bse3DI GCAATG 1 cut(s) 1654
Bse8I GATNNNNATC 2 cut(s) 267, 2064
BseBI CCWGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
BseCI ATCGAT 1 cut(s) 271
BseDI CCNNGG 4 cut(s) 142, 806, 1780, 2401
BseGI GGATG 8 cut(s) 298, 745, 1043, 1070, 1625, 2027, 2239, 2439
BseJI GATNNNNATC 2 cut(s) 267, 2064
BseLI CCNNNNNNNGG 8 cut(s) 52, 698, 844, 865, 1416, 1859, 1881, 2407
BseMI GCAATG 1 cut(s) 1654
BseMII CTCAG 3 cut(s) 351, 1268, 2046
BseNI ACTGG 7 cut(s) 109, 778, 1318, 1542, 1549, 2202, 2589
BseRI GAGGAG 1 cut(s) 1904
BseSI GKGCMC 1 cut(s) 1118
BseXI GCAGC 3 cut(s) 1591, 1601, 2152
BseYI CCCAGC 1 cut(s) 1859
BsgI GTGCAG 1 cut(s) 1590
BshFI GGCC 6 cut(s) 848, 1116, 1541, 1872, 1968, 2400
BshVI ATCGAT 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 2310
BsiSI CCGG 1 cut(s) 2519
BslFI GGGAC 3 cut(s) 390, 1432, 2038
BslI CCNNNNNNNGG 8 cut(s) 52, 698, 844, 865, 1416, 1859, 1881, 2407
BsmAI GTCTC 3 cut(s) 1350, 1780, 1979
BsmFI GGGAC 3 cut(s) 390, 1432, 2038
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 6 cut(s) 848, 1116, 1541, 1872, 1968, 2400
Bso31I GGTCTC 3 cut(s) 1350, 1780, 1979
BsoBI CYCGRG 1 cut(s) 2310
Bsp120I GGGCCC 1 cut(s) 1114
Bsp1286I GDGCHC 2 cut(s) 60, 1118
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 3 cut(s) 191, 845, 1592
BspANI GGCC 6 cut(s) 848, 1116, 1541, 1872, 1968, 2400
BspCNI CTCAG 3 cut(s) 350, 1269, 2045
BspDI ATCGAT 1 cut(s) 271
BspHI TCATGA 2 cut(s) 391, 1300
BspLI GGNNCC 4 cut(s) 378, 1116, 1420, 1873
BspPI GGATC 5 cut(s) 100, 212, 709, 2009, 2264
BspQI GCTCTTC 2 cut(s) 1458, 1971
BspTI CTTAAG 2 cut(s) 766, 1993
BspTNI GGTCTC 3 cut(s) 1350, 1780, 1979
BsrDI GCAATG 1 cut(s) 1654
BsrI ACTGG 7 cut(s) 109, 778, 1318, 1542, 1549, 2202, 2589
BssECI CCNNGG 4 cut(s) 142, 806, 1780, 2401
BssT1I CCWWGG 3 cut(s) 142, 1780, 2401
Bst2UI CCWGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
Bst4CI ACNGT 6 cut(s) 513, 901, 983, 1034, 2114, 2362
Bst6I CTCTTC 5 cut(s) 528, 1458, 1971, 2204, 2359
BstAFI CTTAAG 2 cut(s) 766, 1993
BstC8I GCNNGC 2 cut(s) 1521, 2409
BstDEI CTNAG 8 cut(s) 257, 290, 337, 746, 1172, 1277, 1337, 2032
BstDSI CCRYGG 1 cut(s) 142
BstENI CCTNNNNNAGG 1 cut(s) 2405
BstF5I GGATG 8 cut(s) 298, 745, 1043, 1070, 1625, 2027, 2239, 2439
BstMAI GTCTC 3 cut(s) 1350, 1780, 1979
BstMWI GCNNNNNNNGC 5 cut(s) 353, 1601, 1891, 2495, 2578
BstNI CCWGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
BstNSI RCATGY 3 cut(s) 925, 2140, 2572
BstSCI CCNGG 6 cut(s) 463, 751, 805, 1125, 1492, 2335
BstSFI CTRYAG 2 cut(s) 96, 1596
BstSLI GKGCMC 1 cut(s) 1118
BstV1I GCAGC 3 cut(s) 1591, 1601, 2152
BstV2I GAAGAC 3 cut(s) 171, 1686, 2521
BstX2I RGATCY 4 cut(s) 92, 991, 1273, 2269
BstXI CCANNNNNNTGG 1 cut(s) 116
BstYI RGATCY 4 cut(s) 92, 991, 1273, 2269
Bsu15I ATCGAT 1 cut(s) 271
BsuI GTATCC 1 cut(s) 1795
BsuRI GGCC 6 cut(s) 848, 1116, 1541, 1872, 1968, 2400
BsuTUI ATCGAT 1 cut(s) 271
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 8 cut(s) 298, 745, 1043, 1070, 1625, 2027, 2239, 2439
BtsIMutI CAGTG 4 cut(s) 1356, 1535, 2075, 2269
Cac8I GCNNGC 2 cut(s) 1521, 2409
CaiI CAGNNNCTG 2 cut(s) 710, 1595
CciI TCATGA 2 cut(s) 391, 1300
Cfr13I GGNCC 6 cut(s) 377, 856, 1114, 1115, 1419, 1871
ClaI ATCGAT 1 cut(s) 271
CseI GACGC 1 cut(s) 629
Csp6I GTAC 5 cut(s) 112, 318, 771, 984, 1787
CspCI CAANNNNNGTGG 2 cut(s) 882, 917
CviQI GTAC 5 cut(s) 112, 318, 771, 984, 1787
DdeI CTNAG 8 cut(s) 257, 290, 337, 746, 1172, 1277, 1337, 2032
DraI TTTAAA 1 cut(s) 2377
DraIII CACNNNGTG 1 cut(s) 884
EaeI YGGCCR 2 cut(s) 846, 1539
Eam1104I CTCTTC 5 cut(s) 528, 1458, 1971, 2204, 2359
EarI CTCTTC 5 cut(s) 528, 1458, 1971, 2204, 2359
Eco130I CCWWGG 3 cut(s) 142, 1780, 2401
Eco24I GRGCYC 1 cut(s) 1118
Eco31I GGTCTC 3 cut(s) 1350, 1780, 1979
Eco32I GATATC 1 cut(s) 51
Eco47I GGWCC 3 cut(s) 377, 856, 1419
Eco57I CTGAAG 6 cut(s) 732, 881, 1698, 1973, 2165, 2371
Eco88I CYCGRG 1 cut(s) 2310
EcoNI CCTNNNNNAGG 1 cut(s) 2405
EcoO109I RGGNCCY 2 cut(s) 1114, 1115
EcoRII CCWGG 6 cut(s) 463, 751, 805, 1125, 1492, 2335
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 3 cut(s) 142, 1780, 2401
EcoT22I ATGCAT 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 1118
ErhI CCWWGG 3 cut(s) 142, 1780, 2401
FalI AAGNNNNNCTT 8 cut(s) 440, 472, 1437, 1469, 1773, 1805, 1911, 1943
FaqI GGGAC 3 cut(s) 390, 1432, 2038
FauNDI CATATG 1 cut(s) 2275
Fnu4HI GCNGC 5 cut(s) 846, 1590, 1593, 1605, 2141
FokI GGATG 8 cut(s) 285, 752, 1030, 1057, 1612, 2014, 2246, 2426
FriOI GRGCYC 1 cut(s) 1118
Fsp4HI GCNGC 5 cut(s) 846, 1590, 1593, 1605, 2141
FspBI CTAG 7 cut(s) 83, 558, 989, 1397, 1427, 2280, 2595
GluI GCNGC 5 cut(s) 846, 1590, 1593, 1605, 2141
GsaI CCCAGC 1 cut(s) 1863
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 6 cut(s) 848, 1116, 1541, 1872, 1968, 2400
HapII CCGG 1 cut(s) 2519
HgaI GACGC 1 cut(s) 629
HincII GTYRAC 1 cut(s) 1030
HindII GTYRAC 1 cut(s) 1030
HindIII AAGCTT 3 cut(s) 1224, 1310, 2300
HpaII CCGG 1 cut(s) 2519
HphI GGTGA 9 cut(s) 10, 360, 539, 663, 803, 896, 1724, 1853, 2096
Hpy166II GTNNAC 5 cut(s) 915, 953, 986, 1030, 1356
Hpy8I GTNNAC 5 cut(s) 915, 953, 986, 1030, 1356
Hpy99I CGWCG 1 cut(s) 1461
HpyAV CCTTC 6 cut(s) 59, 500, 1128, 1947, 2198, 2224
HpyCH4III ACNGT 6 cut(s) 513, 901, 983, 1034, 2114, 2362
HpyCH4IV ACGT 2 cut(s) 283, 1132
HpyF10VI GCNNNNNNNGC 5 cut(s) 353, 1601, 1891, 2495, 2578
HpyF3I CTNAG 8 cut(s) 257, 290, 337, 746, 1172, 1277, 1337, 2032
HpySE526I ACGT 2 cut(s) 283, 1132
LguI GCTCTTC 2 cut(s) 1458, 1971
LmnI GCTCC 4 cut(s) 55, 119, 529, 1891
Lsp1109I GCAGC 3 cut(s) 1591, 1601, 2152
LweI GCATC 6 cut(s) 76, 178, 253, 373, 730, 1079
MaeI CTAG 7 cut(s) 83, 558, 989, 1397, 1427, 2280, 2595
MaeII ACGT 2 cut(s) 283, 1132
MaeIII GTNAC 4 cut(s) 16, 241, 513, 641
MflI RGATCY 4 cut(s) 92, 991, 1273, 2269
MhlI GDGCHC 2 cut(s) 60, 1118
MlsI TGGCCA 1 cut(s) 1541
MluNI TGGCCA 1 cut(s) 1541
MlyI GAGTC 3 cut(s) 687, 1702, 2221
MmeI TCCRAC 2 cut(s) 834, 2130
Mox20I TGGCCA 1 cut(s) 1541
Mph1103I ATGCAT 1 cut(s) 388
MroXI GAANNNNTTC 5 cut(s) 1227, 1365, 1452, 1923, 2532
MscI TGGCCA 1 cut(s) 1541
MseI TTAA 7 cut(s) 767, 1664, 1722, 1994, 2376, 2466, 2555
MslI CAYNNNNRTG 3 cut(s) 396, 738, 1630
Msp20I TGGCCA 1 cut(s) 1541
MspA1I CMGCKG 4 cut(s) 305, 1592, 1859, 2143
MspCI CTTAAG 2 cut(s) 766, 1993
MspI CCGG 1 cut(s) 2519
MspR9I CCNGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
MwoI GCNNNNNNNGC 5 cut(s) 353, 1601, 1891, 2495, 2578
NcoI CCATGG 1 cut(s) 142
NdeI CATATG 1 cut(s) 2275
NlaIV GGNNCC 4 cut(s) 378, 1116, 1420, 1873
NmuCI GTSAC 3 cut(s) 16, 513, 641
NsiI ATGCAT 1 cut(s) 388
NspI RCATGY 3 cut(s) 925, 2140, 2572
PagI TCATGA 2 cut(s) 391, 1300
PciI ACATGT 1 cut(s) 921
PciSI GCTCTTC 2 cut(s) 1458, 1971
PcsI WCGNNNNNNNCGW 1 cut(s) 952
PctI GAATGC 1 cut(s) 211
PdmI GAANNNNTTC 5 cut(s) 1227, 1365, 1452, 1923, 2532
PflMI CCANNNNNTGG 1 cut(s) 1859
PfoI TCCNGGA 1 cut(s) 751
PkrI GCNGC 5 cut(s) 847, 1591, 1594, 1606, 2142
PleI GAGTC 3 cut(s) 686, 1701, 2220
PpsI GAGTC 3 cut(s) 686, 1701, 2220
PscI ACATGT 1 cut(s) 921
PshBI ATTAAT 2 cut(s) 1722, 2466
PsiI TTATAA 1 cut(s) 2298
Psp6I CCWGG 6 cut(s) 463, 751, 805, 1125, 1492, 2335
PspFI CCCAGC 1 cut(s) 1859
PspGI CCWGG 6 cut(s) 463, 751, 805, 1125, 1492, 2335
PspN4I GGNNCC 4 cut(s) 378, 1116, 1420, 1873
PspOMI GGGCCC 1 cut(s) 1114
PspPI GGNCC 6 cut(s) 377, 856, 1114, 1115, 1419, 1871
PstNI CAGNNNCTG 2 cut(s) 710, 1595
PsuI RGATCY 4 cut(s) 92, 991, 1273, 2269
PvuII CAGCTG 3 cut(s) 305, 1859, 2143
RsaI GTAC 5 cut(s) 113, 319, 772, 985, 1788
RsaNI GTAC 5 cut(s) 112, 318, 771, 984, 1787
RseI CAYNNNNRTG 3 cut(s) 396, 738, 1630
SapI GCTCTTC 2 cut(s) 1458, 1971
SaqAI TTAA 7 cut(s) 767, 1664, 1722, 1994, 2376, 2466, 2555
SatI GCNGC 5 cut(s) 846, 1590, 1593, 1605, 2141
Sau96I GGNCC 6 cut(s) 377, 856, 1114, 1115, 1419, 1871
ScaI AGTACT 1 cut(s) 772
SchI GAGTC 3 cut(s) 687, 1702, 2221
ScrFI CCNGG 6 cut(s) 465, 753, 807, 1127, 1494, 2337
SduI GDGCHC 2 cut(s) 60, 1118
SfaNI GCATC 6 cut(s) 76, 178, 253, 373, 730, 1079
SfcI CTRYAG 2 cut(s) 96, 1596
SinI GGWCC 3 cut(s) 377, 856, 1419
SmiMI CAYNNNNRTG 3 cut(s) 396, 738, 1630
SmlI CTYRAG 6 cut(s) 766, 1263, 1286, 1982, 1993, 2490
SmoI CTYRAG 6 cut(s) 766, 1263, 1286, 1982, 1993, 2490
SpeI ACTAGT 1 cut(s) 1426
SsiI CCGC 3 cut(s) 191, 845, 1592
SspI AATATT 1 cut(s) 1662
SspMI CTAG 7 cut(s) 83, 558, 989, 1397, 1427, 2280, 2595
StyD4I CCNGG 6 cut(s) 463, 751, 805, 1125, 1492, 2335
StyI CCWWGG 3 cut(s) 142, 1780, 2401
TaaI ACNGT 6 cut(s) 513, 901, 983, 1034, 2114, 2362
TaiI ACGT 2 cut(s) 286, 1135
TaqI TCGA 6 cut(s) 12, 33, 271, 1369, 2169, 2215
TatI WGTACW 3 cut(s) 111, 317, 770
TauI GCSGC 2 cut(s) 848, 1595
Tru1I TTAA 7 cut(s) 767, 1664, 1722, 1994, 2376, 2466, 2555
Tru9I TTAA 7 cut(s) 767, 1664, 1722, 1994, 2376, 2466, 2555
TscAI CASTG 4 cut(s) 1363, 1542, 2075, 2269
TseFI GTSAC 3 cut(s) 16, 513, 641
TseI GCWGC 3 cut(s) 1589, 1604, 2140
Tsp45I GTSAC 3 cut(s) 16, 513, 641
TspGWI ACGGA 1 cut(s) 1462
TspRI CASTG 4 cut(s) 1363, 1542, 2075, 2269
Van91I CCANNNNNTGG 1 cut(s) 1859
Vha464I CTTAAG 2 cut(s) 766, 1993
VpaK11BI GGWCC 3 cut(s) 377, 856, 1419
VspI ATTAAT 2 cut(s) 1722, 2466
XagI CCTNNNNNAGG 1 cut(s) 2405
XapI RAATTY 8 cut(s) 211, 622, 826, 1167, 1296, 1766, 1846, 2183
XceI RCATGY 3 cut(s) 925, 2140, 2572
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XmnI GAANNNNTTC 5 cut(s) 1227, 1365, 1452, 1923, 2532
XspI CTAG 7 cut(s) 83, 558, 989, 1397, 1427, 2280, 2595
ZrmI AGTACT 1 cut(s) 772
Zsp2I ATGCAT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.