RchiOBHm_Chr2g0147671

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017066
Physical Location & Seq
Reverse (-)
65256806 .. 65260144
3339 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2328 bp
ATGTCTCTCTCTATCTCCATCCCTATCTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTAGCTTCAGCTTCTTCAGATTCCAAGACCTTCATAGTTCAAGTCCAACCTGATTCCAAGCCCTCAGTTTTCCCAACCCATAAACACTGGTATGAGTCTTCACTGTCCTCATTATCTTCTGAACCCACACCGTTGATACATACTTACAACACAGTCTTCCATGGCTTCTCTGCCAAGCTCTCTCCTTCCCAAGCCCAAAAGCTCCAGTCCTTGCCTCATGTTTTGGCTCTCATTCCCGAGCAAGTGCGTCGCCTCCACACCACTCGCTCGCCGGAGTTCCTCGGCCTCCGGTCCACTGACACTGCTGGGCTTCTCAAAGAATCGGACTTTGGTTCGGATCTCGTGATCGGAGTTATTGATACCGGAGTTTGGCCCGAGAGGCAGAGCTTCAACGACCACGACCTTAGTCCGGTTCCGTCCAAATGGAAGGGCCAGTGCGTCGCCGGAGAGAACTTTCTGGCTAGTTCGTGTAACCGCAAGCTCATCGGAGCGAGGTACTTCTGCGGCGGGTACGAGTCCACCAACGGCAAGATGAACCAGACTACGGAGTTTCGATCTCCCAGAGACACTGACGGCCACGGCACTCACACCGCCTCGATCGCCGCCGGCCGTTACGTCTTTCCGGCGTCTACTCTCGGCTATGCTAAAGGCGTCGCCGCCGGCATGGCTCCCAAGGCCCGGCTAGCAGCCTACAAGGTCTGCTGGAACGCCGGCTGTTACGACTCTGACATTCTCGCCGCCTTCGACTCCGCCGTCGCCGACGGATGCGACGTCGTCTCCCTCAGCGTCGGCGGGGTCGTTGTCCCCTACTACCTTGACGCCATTGCCATCGGAGCCTTTGGAGCCTCAGACGCCGGAGTATTCGTCTCGGCCTCGGCTGGAAATGGCGGCCCCGGTGGGCTCACGGTGACCAATGTAGCTCCATGGGTCACCACCGTCGGCGCCGGAACAATCGACCGTGACTTCCCTGCTGATGTTAAGCTTGGCAACGGCAGAATCATCCCCGGCATGAGTGTTTATGGCGGGCCGGGTCTCCCCCCGGGTCGGATGTTCCCGTTAGTTTACGCCGGCAGCGAAGGCGGTGATGGGTACTCCTCTTCTCTATGCCTAGAAGGTTCTCTTGACCAAAGGCTGGTGAAGAACAAGATAGTTGTTTGCGACAGAGGAATCAATTCAAGAGCTGCAAAAGGTGAGGTGGTGAAGAAAGCCGGAGGAGTTGGCATGATTCTTGCAAATGGTGTCTTCGACGGTGAAGGACTAGTAGCCGACTGCCACGTCTTACCTGCCACCGCCGTCGCTGCTTCCACCGGTGACGAAATAAGGCAGTACATAACAGCTGCGTCCAAGTCCAAATCACCACCAACAGCTACCATTTTGTTCAAGGGTACTCGAATCCGTGTCAAACCAGCACCGGTAGTTGCCTCATTTTCGGCCCGTGGCCCCAACCCGGAAGCGCCGGAAATACTGAAGCCGGATGTGATAGCACCTGGACTGAACATACTTGCAGCTTGGCCGGATAAAGTGGGGCCATCAGGCATTCCTTCGGATAAGAGAACCACCGAGTTCAACATTCTTTCGGGTACGTCAATGGCTTGCCCACACGTTTCAGGTCTGGCCGCCTTGTTGAAGGCGGCCCACCCGGAATGGAGTCCGGCTGCTATTAGATCGGCTCTAATGACGACAGCCTACACCGTCGACAATAGAGGCGAGACGATGCTTGACGAGTCTAGTGGGAATACTTCGACAGTTATGGACTTTGGGGCTGGCCATGTCCATCCGCAAAAGGCAATGGACCCTGGATTGGTTTACGACATAAGTTCATCCGATTATGTCGATTTCTTGTGTAATTCCAATTACACGACTAAGAACATCCAGGTGATCACTAGGAAAGTAGCAAATTGCAATGGGGCAAAGAGGGCAGGCCATTCGGGGAATCTGAATTACCCATCATTGTCTGTGGTTTTCCAGCAATACGGAAAGCGAAAGATGTCTACGCATTTTATTCGGACGGTCACAAATGTTGGGGACCCGAAATCGGTTTATCAGGTGACGATCAGGCCACCCCGGGGAATGTCGGTGACAGTGAAGCCGGAGAAGCTTGCGTTTAGGAGGGTGGGGCAGAAATTGAGCTTCCTTGTGAGGGTTCAAGCTAGGGAAGTCAAGCTTTCTCCTGGAAGTAGTAGCATGAAGAGTGGGTCTATACTGTGGTCAGATGGGAAACACACTGTGACTAGTCCTTTGGTTGTGACAATGCAACAACCTCTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

775

Amino Acids

82.24

Weight (kDa)

9.07

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 29 - 105 5.4e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 130 - 597 1e-48 Subtilase family
PA PF02225 371 - 459 2e-11 PA domain
fn3_6 PF17766 664 - 768 5.3e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14240
fragaria_vesca FvH4_6g35680
malus_domestica MD17G1152800.v1.1
prunus_persica Prupe.3G032200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0147671
rosa_laevigata RLG00000020269
rosa_multiflora Rmu_sc0000308.1_g000011
rosa_roxburghii Rroxscaffold_2G00099400
rosa_rugosa Rorug02G0400700
rosa_samantha Rh2AG458100 Rh2BG470800 Rh2CG445500
rosa_wichuraiana Rw2G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 809, 1331
AatII GACGTC 1 cut(s) 831
Acc36I ACCTGC 1 cut(s) 1348
AccB1I GGYRCC 1 cut(s) 998
AccB7I CCANNNNNTGG 1 cut(s) 1189
AccI GTMKAC 3 cut(s) 686, 1752, 2048
AclWI GGATC 1 cut(s) 402
AcoI YGGCCR 5 cut(s) 631, 664, 1568, 1671, 1822
AcuI CTGAAG 3 cut(s) 48, 57, 1544
AcyI GRCGYC 6 cut(s) 683, 708, 828, 876, 909, 999
AdeI CACNNNGTG 1 cut(s) 2284
AfaI GTAC 6 cut(s) 554, 569, 1148, 1385, 1444, 1639
AflIII ACRYGT 1 cut(s) 1657
AgeI ACCGGT 2 cut(s) 1364, 1468
AgsI TTSAA 7 cut(s) 98, 448, 1233, 1438, 1624, 1684, 2204
AhlI ACTAGT 2 cut(s) 1315, 2288
AjiI CACGTC 1 cut(s) 1333
AjnI CCWGG 4 cut(s) 1543, 1852, 1929, 2227
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
Alw26I GTCTC 6 cut(s) 9, 615, 838, 928, 1094, 1760
AlwI GGATC 1 cut(s) 402
Ama87I CYCGRG 4 cut(s) 293, 431, 1096, 2121
ApeKI GCWGC 7 cut(s) 743, 1128, 1238, 1355, 1394, 1562, 1712
ArsI GACNNNNNNTTYG 2 cut(s) 2278, 2310
AsiGI ACCGGT 2 cut(s) 1364, 1468
Asp700I GAANNNNTTC 1 cut(s) 1228
AspLEI GCGC 2 cut(s) 1001, 1513
AsuNHI GCTAGC 1 cut(s) 739
AvaI CYCGRG 4 cut(s) 293, 431, 1096, 2121
AvaII GGWCC 3 cut(s) 348, 1849, 2083
BaeI ACNNNNGTAYC 2 cut(s) 408, 441
BalI TGGCCA 1 cut(s) 1824
BanI GGYRCC 1 cut(s) 998
BanII GRGCYC 1 cut(s) 960
BauI CACGAG 1 cut(s) 398
BbsI GAAGAC 3 cut(s) 147, 205, 1291
BbvCI CCTCAGC 1 cut(s) 839
BbvI GCAGC 7 cut(s) 755, 1140, 1225, 1342, 1381, 1574, 1699
BccI CCATC 7 cut(s) 26, 893, 1136, 1594, 1839, 2011, 2264
BceAI ACGGC 7 cut(s) 598, 646, 651, 652, 794, 1063, 1334
BcgI CGANNNNNNTGC 8 cut(s) 287, 321, 523, 557, 1965, 1999, 2042, 2076
BciT130I CCWGG 4 cut(s) 1545, 1854, 1931, 2229
BclI TGATCA 1 cut(s) 1935
BcoDI GTCTC 6 cut(s) 9, 615, 838, 928, 1094, 1760
BcuI ACTAGT 2 cut(s) 1315, 2288
BfaI CTAG 9 cut(s) 519, 740, 1166, 1316, 1785, 1941, 2208, 2289, 2321
BfoI RGCGCY 2 cut(s) 1002, 1514
BfuAI ACCTGC 1 cut(s) 1348
BglI GCCNNNNNGGC 2 cut(s) 436, 722
Bme18I GGWCC 3 cut(s) 348, 1849, 2083
BmeT110I CYCGRG 4 cut(s) 293, 431, 1096, 2121
BmgBI CACGTC 1 cut(s) 1333
BmsI GCATC 2 cut(s) 812, 1761
BmtI GCTAGC 1 cut(s) 743
BoxI GACNNNNGTC 1 cut(s) 462
BpiI GAAGAC 3 cut(s) 147, 205, 1291
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 839
BsaHI GRCGYC 6 cut(s) 683, 708, 828, 876, 909, 999
BsaI GGTCTC 1 cut(s) 1094
BsaWI WCCGGW 5 cut(s) 345, 419, 466, 1364, 1468
BsaXI ACNNNNNCTCC 4 cut(s) 818, 848, 906, 936
Bse118I RCCGGY 6 cut(s) 662, 716, 767, 1124, 1364, 1468
Bse1I ACTGG 3 cut(s) 149, 262, 490
Bse3DI GCAATG 3 cut(s) 879, 1851, 1966
BseBI CCWGG 4 cut(s) 1545, 1854, 1931, 2229
BseGI GGATG 8 cut(s) 18, 827, 1056, 1110, 1537, 1831, 1877, 1926
BseMI GCAATG 3 cut(s) 879, 1851, 1966
BseMII CTCAG 3 cut(s) 135, 853, 918
BseNI ACTGG 3 cut(s) 149, 262, 490
BseRI GAGGAG 2 cut(s) 1141, 1284
BseX3I CGGCCG 1 cut(s) 664
BseXI GCAGC 7 cut(s) 755, 1140, 1225, 1342, 1381, 1574, 1699
BseYI CCCAGC 1 cut(s) 362
Bsh1285I CGRYCG 3 cut(s) 657, 667, 1015
BshNI GGYRCC 1 cut(s) 998
BshTI ACCGGT 2 cut(s) 1364, 1468
BsiEI CGRYCG 3 cut(s) 657, 667, 1015
BsiHKCI CYCGRG 4 cut(s) 293, 431, 1096, 2121
BslFI GGGAC 2 cut(s) 845, 2096
BsmAI GTCTC 6 cut(s) 9, 615, 838, 928, 1094, 1760
BsmBI CGTCTC 3 cut(s) 838, 928, 1760
BsmFI GGGAC 2 cut(s) 845, 2096
BsmI GAATGC 1 cut(s) 1593
Bso31I GGTCTC 1 cut(s) 1094
BsoBI CYCGRG 4 cut(s) 293, 431, 1096, 2121
Bsp1286I GDGCHC 1 cut(s) 960
Bsp143I GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
Bsp19I CCATGG 2 cut(s) 217, 980
BspCNI CTCAG 3 cut(s) 134, 852, 917
BspMI ACCTGC 1 cut(s) 1348
BspOI GCTAGC 1 cut(s) 743
BspPI GGATC 1 cut(s) 402
BspT107I GGYRCC 1 cut(s) 998
BspTNI GGTCTC 1 cut(s) 1094
BsrDI GCAATG 3 cut(s) 879, 1851, 1966
BsrFI RCCGGY 6 cut(s) 662, 716, 767, 1124, 1364, 1468
BsrI ACTGG 3 cut(s) 149, 262, 490
BssAI RCCGGY 6 cut(s) 662, 716, 767, 1124, 1364, 1468
BssMI GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
BssNI GRCGYC 6 cut(s) 683, 708, 828, 876, 909, 999
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 3 cut(s) 217, 729, 980
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 4 cut(s) 1545, 1854, 1931, 2229
Bst6I CTCTTC 3 cut(s) 38, 1159, 2240
BstACI GRCGYC 6 cut(s) 683, 708, 828, 876, 909, 999
BstDEI CTNAG 6 cut(s) 58, 121, 461, 839, 904, 1920
BstDSI CCRYGG 4 cut(s) 217, 634, 980, 1492
BstEII GGTNACC 2 cut(s) 964, 985
BstF5I GGATG 8 cut(s) 18, 827, 1056, 1110, 1537, 1831, 1877, 1926
BstH2I RGCGCY 2 cut(s) 1002, 1514
BstHHI GCGC 2 cut(s) 1001, 1513
BstKTI GATC 7 cut(s) 397, 405, 614, 657, 1724, 1938, 2112
BstMAI GTCTC 6 cut(s) 9, 615, 838, 928, 1094, 1760
BstMBI GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
BstMCI CGRYCG 3 cut(s) 657, 667, 1015
BstNI CCWGG 4 cut(s) 1545, 1854, 1931, 2229
BstPAI GACNNNNGTC 1 cut(s) 462
BstPI GGTNACC 2 cut(s) 964, 985
BstV1I GCAGC 7 cut(s) 755, 1140, 1225, 1342, 1381, 1574, 1699
BstV2I GAAGAC 3 cut(s) 147, 205, 1291
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BstZI CGGCCG 1 cut(s) 664
BtgI CCRYGG 4 cut(s) 217, 634, 980, 1492
BtrI CACGTC 1 cut(s) 1333
BtsCI GGATG 8 cut(s) 18, 827, 1056, 1110, 1537, 1831, 1877, 1926
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 8 cut(s) 142, 158, 351, 357, 497, 624, 2145, 2280
BveI ACCTGC 1 cut(s) 1348
CfoI GCGC 2 cut(s) 1001, 1513
Cfr10I RCCGGY 6 cut(s) 662, 716, 767, 1124, 1364, 1468
Cfr9I CCCGGG 2 cut(s) 1096, 2121
CseI GACGC 8 cut(s) 293, 484, 672, 697, 832, 884, 917, 1386
Csp6I GTAC 6 cut(s) 553, 568, 1147, 1384, 1443, 1638
CspAI ACCGGT 2 cut(s) 1364, 1468
CviAII CATG 8 cut(s) 218, 275, 721, 981, 1066, 1279, 1826, 2242
CviQI GTAC 6 cut(s) 553, 568, 1147, 1384, 1443, 1638
DdeI CTNAG 6 cut(s) 58, 121, 461, 839, 904, 1920
DinI GGCGCC 1 cut(s) 1000
DpnI GATC 7 cut(s) 396, 404, 613, 656, 1723, 1937, 2111
DpnII GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
DraIII CACNNNGTG 1 cut(s) 2284
DrdI GACNNNNNNGTC 2 cut(s) 809, 1331
DseDI GACNNNNNNGTC 2 cut(s) 809, 1331
EaeI YGGCCR 5 cut(s) 631, 664, 1568, 1671, 1822
EagI CGGCCG 1 cut(s) 664
Eam1104I CTCTTC 3 cut(s) 38, 1159, 2240
EarI CTCTTC 3 cut(s) 38, 1159, 2240
EciI GGCGGA 1 cut(s) 796
EclXI CGGCCG 1 cut(s) 664
Eco130I CCWWGG 3 cut(s) 217, 729, 980
Eco24I GRGCYC 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 1094
Eco47I GGWCC 3 cut(s) 348, 1849, 2083
Eco52I CGGCCG 1 cut(s) 664
Eco57I CTGAAG 3 cut(s) 48, 57, 1544
Eco88I CYCGRG 4 cut(s) 293, 431, 1096, 2121
Eco91I GGTNACC 2 cut(s) 964, 985
EcoO109I RGGNCCY 1 cut(s) 2083
EcoO65I GGTNACC 2 cut(s) 964, 985
EcoRII CCWGG 4 cut(s) 1543, 1852, 1929, 2227
EcoT14I CCWWGG 3 cut(s) 217, 729, 980
EcoT38I GRGCYC 1 cut(s) 960
EgeI GGCGCC 1 cut(s) 1000
EheI GGCGCC 1 cut(s) 1000
ErhI CCWWGG 3 cut(s) 217, 729, 980
Esp3I CGTCTC 3 cut(s) 838, 928, 1760
FaeI CATG 8 cut(s) 221, 278, 724, 984, 1069, 1282, 1829, 2245
FalI AAGNNNNNCTT 4 cut(s) 1161, 1193, 2205, 2237
FaqI GGGAC 2 cut(s) 845, 2096
FatI CATG 8 cut(s) 217, 274, 720, 980, 1065, 1278, 1825, 2241
FauI CCCGC 3 cut(s) 557, 842, 1073
FbaI TGATCA 1 cut(s) 1935
FblI GTMKAC 3 cut(s) 686, 1752, 2048
FokI GGATG 8 cut(s) 5, 834, 1043, 1117, 1544, 1818, 1864, 1913
FriOI GRGCYC 1 cut(s) 960
FspBI CTAG 9 cut(s) 519, 740, 1166, 1316, 1785, 1941, 2208, 2289, 2321
GlaI GCGC 2 cut(s) 1000, 1512
GsaI CCCAGC 1 cut(s) 366
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 2 cut(s) 1002, 1514
HgaI GACGC 8 cut(s) 293, 484, 672, 697, 832, 884, 917, 1386
HhaI GCGC 2 cut(s) 1001, 1513
Hin1I GRCGYC 6 cut(s) 683, 708, 828, 876, 909, 999
Hin1II CATG 8 cut(s) 221, 278, 724, 984, 1069, 1282, 1829, 2245
Hin6I GCGC 2 cut(s) 999, 1511
HinP1I GCGC 2 cut(s) 999, 1511
HincII GTYRAC 1 cut(s) 1753
HindII GTYRAC 1 cut(s) 1753
HindIII AAGCTT 3 cut(s) 1037, 2153, 2219
Hpy166II GTNNAC 7 cut(s) 351, 576, 687, 1120, 1753, 1864, 2049
Hpy188III TCNNGA 4 cut(s) 293, 400, 1178, 1233
Hpy8I GTNNAC 7 cut(s) 351, 576, 687, 1120, 1753, 1864, 2049
HpyAV CCTTC 9 cut(s) 97, 252, 478, 808, 1127, 1163, 1304, 1608, 1678
HpyCH4IV ACGT 5 cut(s) 672, 828, 1332, 1640, 1659
HpyCH4V TGCA 5 cut(s) 1241, 1289, 1562, 1959, 2311
HpyF3I CTNAG 6 cut(s) 58, 121, 461, 839, 904, 1920
HpySE526I ACGT 5 cut(s) 672, 828, 1332, 1640, 1659
Hsp92I GRCGYC 6 cut(s) 683, 708, 828, 876, 909, 999
Hsp92II CATG 8 cut(s) 221, 278, 724, 984, 1069, 1282, 1829, 2245
HspAI GCGC 2 cut(s) 999, 1511
KasI GGCGCC 1 cut(s) 998
KflI GGGWCCC 1 cut(s) 2083
KroI GCCGGC 4 cut(s) 662, 716, 767, 1124
KroNI GCCGGC 4 cut(s) 664, 718, 769, 1126
Ksp22I TGATCA 1 cut(s) 1935
Kzo9I GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
LmnI GCTCC 6 cut(s) 264, 545, 730, 890, 899, 982
Lsp1109I GCAGC 7 cut(s) 755, 1140, 1225, 1342, 1381, 1574, 1699
LweI GCATC 2 cut(s) 812, 1761
MaeI CTAG 9 cut(s) 519, 740, 1166, 1316, 1785, 1941, 2208, 2289, 2321
MaeII ACGT 5 cut(s) 672, 828, 1332, 1640, 1659
MalI GATC 7 cut(s) 396, 404, 613, 656, 1723, 1937, 2111
MboI GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 1 cut(s) 960
MlsI TGGCCA 1 cut(s) 1824
MluCI AATT 6 cut(s) 1228, 1903, 1909, 1954, 1996, 2180
MluNI TGGCCA 1 cut(s) 1824
Mly113I GGCGCC 1 cut(s) 999
MlyI GAGTC 6 cut(s) 161, 581, 773, 797, 1714, 1790
MmeI TCCRAC 2 cut(s) 127, 1082
Mox20I TGGCCA 1 cut(s) 1824
MroNI GCCGGC 4 cut(s) 662, 716, 767, 1124
MroXI GAANNNNTTC 1 cut(s) 1228
MscI TGGCCA 1 cut(s) 1824
MseI TTAA 1 cut(s) 1035
MslI CAYNNNNRTG 2 cut(s) 147, 1931
Msp20I TGGCCA 1 cut(s) 1824
MspA1I CMGCKG 1 cut(s) 1394
Mva1269I GAATGC 1 cut(s) 1593
MvaI CCWGG 4 cut(s) 1545, 1854, 1931, 2229
NaeI GCCGGC 4 cut(s) 664, 718, 769, 1126
NarI GGCGCC 1 cut(s) 999
NcoI CCATGG 2 cut(s) 217, 980
NdeII GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
NgoMIV GCCGGC 4 cut(s) 662, 716, 767, 1124
NheI GCTAGC 1 cut(s) 739
NlaIII CATG 8 cut(s) 221, 278, 724, 984, 1069, 1282, 1829, 2245
NmeAIII GCCGAG 4 cut(s) 318, 672, 905, 911
NmuCI GTSAC 9 cut(s) 964, 985, 1016, 1367, 2068, 2104, 2134, 2284, 2302
PcsI WCGNNNNNNNCGW 5 cut(s) 798, 807, 825, 852, 1128
PctI GAATGC 1 cut(s) 1593
PdiI GCCGGC 4 cut(s) 664, 718, 769, 1126
PdmI GAANNNNTTC 1 cut(s) 1228
PfeI GAWTC 8 cut(s) 77, 110, 377, 1053, 1224, 1282, 1449, 1990
PflFI GACNNNGTC 1 cut(s) 830
PflMI CCANNNNNTGG 1 cut(s) 1189
PfoI TCCNGGA 1 cut(s) 2227
PinAI ACCGGT 2 cut(s) 1364, 1468
Ple19I CGATCG 1 cut(s) 657
PleI GAGTC 6 cut(s) 160, 580, 773, 797, 1713, 1789
PluTI GGCGCC 1 cut(s) 1002
PpsI GAGTC 6 cut(s) 160, 580, 773, 797, 1713, 1789
PpuMI RGGWCCY 1 cut(s) 2083
PshAI GACNNNNGTC 1 cut(s) 462
Psp5II RGGWCCY 1 cut(s) 2083
Psp6I CCWGG 4 cut(s) 1543, 1852, 1929, 2227
PspEI GGTNACC 2 cut(s) 964, 985
PspFI CCCAGC 1 cut(s) 362
PspGI CCWGG 4 cut(s) 1543, 1852, 1929, 2227
PspPPI RGGWCCY 1 cut(s) 2083
PsuI RGATCY 1 cut(s) 394
PsyI GACNNNGTC 1 cut(s) 830
PvuI CGATCG 1 cut(s) 657
PvuII CAGCTG 1 cut(s) 1394
RsaI GTAC 6 cut(s) 554, 569, 1148, 1385, 1444, 1639
RsaNI GTAC 6 cut(s) 553, 568, 1147, 1384, 1443, 1638
RseI CAYNNNNRTG 2 cut(s) 147, 1931
SalI GTCGAC 1 cut(s) 1751
SaqAI TTAA 1 cut(s) 1035
Sau3AI GATC 7 cut(s) 394, 402, 611, 654, 1721, 1935, 2109
SchI GAGTC 6 cut(s) 161, 581, 773, 797, 1714, 1790
SduI GDGCHC 1 cut(s) 960
SfaNI GCATC 2 cut(s) 812, 1761
SfoI GGCGCC 1 cut(s) 1000
SgrAI CRCCGGYG 1 cut(s) 1364
SinI GGWCC 3 cut(s) 348, 1849, 2083
SmaI CCCGGG 2 cut(s) 1098, 2123
SmiMI CAYNNNNRTG 2 cut(s) 147, 1931
SpeI ACTAGT 2 cut(s) 1315, 2288
Sse9I AATT 6 cut(s) 1228, 1903, 1909, 1954, 1996, 2180
SspDI GGCGCC 1 cut(s) 998
SspMI CTAG 9 cut(s) 519, 740, 1166, 1316, 1785, 1941, 2208, 2289, 2321
StyI CCWWGG 3 cut(s) 217, 729, 980
TaiI ACGT 5 cut(s) 675, 831, 1335, 1643, 1662
TaqI TCGA 9 cut(s) 610, 653, 801, 1011, 1302, 1447, 1752, 1799, 1890
TasI AATT 6 cut(s) 1228, 1903, 1909, 1954, 1996, 2180
TatI WGTACW 1 cut(s) 1383
TauI GCSGC 7 cut(s) 564, 662, 716, 797, 948, 1676, 1691
TfiI GAWTC 8 cut(s) 77, 110, 377, 1053, 1224, 1282, 1449, 1990
Tru1I TTAA 1 cut(s) 1035
Tru9I TTAA 1 cut(s) 1035
TscAI CASTG 8 cut(s) 149, 165, 358, 364, 497, 631, 2145, 2287
TseFI GTSAC 9 cut(s) 964, 985, 1016, 1367, 2068, 2104, 2134, 2284, 2302
TseI GCWGC 7 cut(s) 743, 1128, 1238, 1355, 1394, 1562, 1712
Tsp45I GTSAC 9 cut(s) 964, 985, 1016, 1367, 2068, 2104, 2134, 2284, 2302
TspDTI ATGAA 4 cut(s) 79, 605, 1866, 2258
TspGWI ACGGA 5 cut(s) 462, 617, 834, 1442, 2046
TspMI CCCGGG 2 cut(s) 1096, 2121
TspRI CASTG 8 cut(s) 149, 165, 358, 364, 497, 631, 2145, 2287
Tth111I GACNNNGTC 1 cut(s) 830
Van91I CCANNNNNTGG 1 cut(s) 1189
VpaK11BI GGWCC 3 cut(s) 348, 1849, 2083
XmaI CCCGGG 2 cut(s) 1096, 2121
XmiI GTMKAC 3 cut(s) 686, 1752, 2048
XmnI GAANNNNTTC 1 cut(s) 1228
XspI CTAG 9 cut(s) 519, 740, 1166, 1316, 1785, 1941, 2208, 2289, 2321
ZraI GACGTC 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.