Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0015780

Physical Location & Seq

Genomic Coordinates Reverse (-)

65484066 .. 65487542

Gene Structure 3477 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGAGTTCCTTTAAGGGCTACGGCAAAGTCGACGAGCTCGAAGAGCAAGCCTTCCGTCTCAAAACCCGCAAGCGCTTGCTCATCGTCACCGTCTCCGTCATTGTCCTCTTGGCCGTCATCATCGGCGCCGTCACCGGCACAGTCATCCACAACCGCAACAGCTCCTCTTCGCCGTCGCCGAACACCGAACCCGCCACCCAGTTGACTCCCTCCGCTTCCTTAAAAGCCGTCTGCGGCGTCACCCAGTACCCCAACTCGTGCTTCTCGAGCATCTCTTCTCTCAAAAACTCCAACACCACCGACCCGGAAGCGCTGTTCAAGCTGTCCCTGCATGTCGCCATTAACGAGGCCTCGAAGCTCAAAGACTTGCCTTCAACAATAAAGATCGGAAATGATAGCAAACTCGAAAGCGCTATTAAGGTCTGCGAGACCGTTTTCGATGATGCTGTGGATAAGCTTAACGATTCGCTCTCTGCAATGGATGTCAGTTCCGGCGAGAAGCTGTTGTCCGAGGCGAAGATCAATGACCTGAAGACGTGGATCAGCGCCACCATGACTGACCAAGAGACCTGCCTGGACTCTCTTGAAGACATCAATTCGACTTACGTTCAGGAATTCAGGGATGCTATGCAGAACTCCACAGAATACGCCAGCAACAGCCTGGCCATTGTGGCGAAAATTCTGGGGTTATTGTCCAATTTGAACATTCCGGTTCACCGGAGATTGCTCGGGTACGGAGGAGAAAGATCCTCTGTACCCGAGTTCCCGGGCTGGGTCAGCCCGGGAGACAGGAGGCTGCTGCAGGAGCAGAACCTGACCGCGCACGTGGTTGTGGCCAAGGATGGGACTGGGGATTTTAAGACCATTCAGGAGGCTGTGAATGCTGTGCCCAAGAAGAACTTGACCAGGTTTGTGATATATGTGAAGCAAGGGACGTACGAAGAAAATGTGATCATGGACAAGAGCACATGGAATGTGATGATGTATGGAGATGGCAAGACCAAGACCATTGTTTCTGGGAAGTTGAACTATGTGGATGGGACTGCAACTTTCAACACAGCCACTTTTGCTGTAGCAGGAAGAGGGTTCATTGCGAAAGATATAGGGTTCATTAACACTGCTGGAGCTGCAAAGCACCAAGCGGTGGCATTCCGATCTGGTTCCGACCAATCTGTGTTCTACAGATGCTCATTTGATGCATTCCAGGACACTCTATATGCTCACTCAAATCGACAATTCTATCGTGACTGTGACATCACTGGTACCATTGACTTCATGTTTGGCAATGCAGCTGTGGTGTTCCAGAACTGTAACATCAAGCCGAGGCAACCAATGCCAAACCAGTTCAACACAATCACTGCTCAGGGAAAGAAGGACCCCAATCAAAACACTGGCATTTCAATTCAGAAGTGCACAATCTCTCCACTCGAAAGCAACATGACGGCTCCAACATATCTTGGTAGACCTTGGAAGAACTTTTCCACCACGGTTTTCTTAGAATCCAATATTGATTCGTTCTTGAACCCATCGGGGTGGACAGAATGGATTCCCAATGTTGATCCACCTAACACCATATTTTACGCAGAATACCAAAATAGTGGACCAGGATCGAGTGTGGATAAAAGGGTTACATGGGCTGGTTACAGGCCCTCTCTCACAGATACTGAAGCAGCCAAGTTCACAGTTGAGTCCTTCATCCAAGGTAATGAATGGTTGCCTCAGACTAATGTGAATTTTGATTCATCCCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0030054 GO:0030234 GO:0030599 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0051722 GO:0051723 GO:0052689 GO:0055044 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

583

Amino Acids

64.05

Weight (kDa)

5.84

Isoelectric Point (pI)

32.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	73 - 223	2e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	275 - 569	7e-141	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53840 AT3G14300
fragaria_vesca	FvH4_6g35820
malus_domestica	MD09G1172900.v1.1 MD17G1154400.v1.1
rosa_chinensis	RchiOBHm_Chr2g0147861
rosa_laevigata	RLG00000020286
rosa_multiflora	Rmu_sc0000308.1_g000054
rosa_roxburghii	Rroxscaffold_2G00099260
rosa_rugosa	Rorug02G0402500
rosa_samantha	Rh2AG459400 Rh2BG472800 Rh2CG446800 Rh2DG481700
rosa_wichuraiana	Rw2G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	578
Acc65I	GGTACC	1 cut(s)	1262
AccB1I	GGYRCC	2 cut(s)	125, 1262
AccB7I	CCANNNNNTGG	1 cut(s)	1144
AccI	GTMKAC	2 cut(s)	30, 1462
AccII	CGCG	1 cut(s)	821
AciI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 819, 1142
AclWI	GGATC	4 cut(s)	548, 741, 1553, 1615
AcoI	YGGCCR	3 cut(s)	111, 663, 834
AcsI	RAATTY	3 cut(s)	614, 678, 1732
AcuI	CTGAAG	2 cut(s)	551, 1686
AcvI	CACGTG	1 cut(s)	826
AcyI	GRCGYC	2 cut(s)	126, 237
AfaI	GTAC	5 cut(s)	248, 734, 756, 938, 1264
AfeI	AGCGCT	3 cut(s)	74, 312, 412
AfiI	CCNNNNNNNGG	3 cut(s)	772, 843, 1144
AgsI	TTSAA	9 cut(s)	319, 375, 587, 703, 1027, 1054, 1348, 1401, 1522
AjiI	CACGTC	1 cut(s)	537
AjnI	CCWGG	5 cut(s)	573, 660, 905, 1203, 1603
AjuI	GAANNNNNNNTTGG	2 cut(s)	245, 277
AloI	GAACNNNNNNTCC	2 cut(s)	299, 331
AluBI	AGCT	8 cut(s)	37, 162, 322, 358, 457, 502, 1127, 1292
AluI	AGCT	8 cut(s)	37, 162, 322, 358, 457, 502, 1127, 1292
Alw21I	GWGCWC	3 cut(s)	39, 968, 1415
Alw26I	GTCTC	5 cut(s)	62, 97, 422, 560, 780
Alw44I	GTGCAC	1 cut(s)	1411
AlwI	GGATC	4 cut(s)	548, 741, 1553, 1615
AlwNI	CAGNNNCTG	2 cut(s)	814, 1664
Ama87I	CYCGRG	5 cut(s)	265, 728, 758, 766, 781
Aor51HI	AGCGCT	3 cut(s)	74, 312, 412
AoxI	GGCC	5 cut(s)	111, 348, 663, 834, 1646
ApaLI	GTGCAC	1 cut(s)	1411
ApeKI	GCWGC	5 cut(s)	796, 799, 1127, 1289, 1670
ApoI	RAATTY	3 cut(s)	614, 678, 1732
ArsI	GACNNNNNNTTYG	2 cut(s)	1262, 1294
Asp700I	GAANNNNTTC	1 cut(s)	1545
Asp718I	GGTACC	1 cut(s)	1262
AspLEI	GCGC	6 cut(s)	75, 128, 313, 413, 548, 823
AspS9I	GGNCC	3 cut(s)	1375, 1601, 1647
AsuC2I	CCSGG	5 cut(s)	305, 767, 768, 782, 783
AsuHPI	GGTGA	4 cut(s)	79, 124, 232, 707
AvaI	CYCGRG	5 cut(s)	265, 728, 758, 766, 781
AvaII	GGWCC	2 cut(s)	1375, 1601
BaeGI	GKGCMC	2 cut(s)	891, 1415
BalI	TGGCCA	2 cut(s)	665, 836
BanI	GGYRCC	2 cut(s)	125, 1262
BanII	GRGCYC	1 cut(s)	39
BauI	CACGAG	1 cut(s)	256
BbrPI	CACGTG	1 cut(s)	826
BbsI	GAAGAC	2 cut(s)	539, 594
Bbv12I	GWGCWC	3 cut(s)	39, 968, 1415
BbvI	GCAGC	5 cut(s)	783, 786, 1114, 1301, 1682
BccI	CCATC	4 cut(s)	836, 986, 1031, 1534
BceAI	ACGGC	6 cut(s)	37, 98, 113, 157, 212, 1458
BciT130I	CCWGG	5 cut(s)	575, 662, 907, 1205, 1605
BclI	TGATCA	1 cut(s)	951
BcnI	CCSGG	5 cut(s)	305, 767, 768, 782, 783
BcoDI	GTCTC	5 cut(s)	62, 97, 422, 560, 780
BfmI	CTRYAG	3 cut(s)	800, 1071, 1180
BfoI	RGCGCY	5 cut(s)	76, 129, 314, 414, 549
BfuAI	ACCTGC	1 cut(s)	578
BglI	GCCNNNNNGGC	1 cut(s)	671
BisI	GCNGC	6 cut(s)	235, 797, 800, 1128, 1290, 1671
BlsI	GCNGC	6 cut(s)	236, 798, 801, 1129, 1291, 1672
Bme18I	GGWCC	2 cut(s)	1375, 1601
BmeT110I	CYCGRG	5 cut(s)	265, 728, 758, 766, 781
BmgBI	CACGTC	1 cut(s)	537
BmgT120I	GGNCC	3 cut(s)	1375, 1601, 1647
BmiI	GGNNCC	5 cut(s)	127, 1162, 1264, 1377, 1446
BmrI	ACTGGG	3 cut(s)	193, 238, 858
BmsI	GCATC	5 cut(s)	279, 433, 613, 1175, 1186
BmuI	ACTGGG	3 cut(s)	193, 238, 858
BpiI	GAAGAC	2 cut(s)	539, 594
BpmI	CTGGAG	1 cut(s)	1143
Bpu10I	CCTNAGC	1 cut(s)	1362
BpuMI	CCSGG	5 cut(s)	305, 767, 768, 782, 783
BsaAI	YACGTR	1 cut(s)	826
BsaHI	GRCGYC	2 cut(s)	126, 237
BsaI	GGTCTC	2 cut(s)	422, 560
BsaJI	CCNNGG	8 cut(s)	510, 766, 781, 837, 1322, 1466, 1485, 1699
BsaWI	WCCGGW	2 cut(s)	709, 717
BsaXI	ACNNNNNCTCC	2 cut(s)	1405, 1435
Bsc4I	CCNNNNNNNGG	3 cut(s)	772, 843, 1144
Bse118I	RCCGGY	1 cut(s)	134
Bse1I	ACTGG	6 cut(s)	199, 244, 853, 1264, 1342, 1396
Bse3DI	GCAATG	3 cut(s)	483, 1089, 1291
BseBI	CCWGG	5 cut(s)	575, 662, 907, 1205, 1605
BseDI	CCNNGG	8 cut(s)	510, 766, 781, 837, 1322, 1466, 1485, 1699
BseGI	GGATG	7 cut(s)	144, 487, 628, 847, 1042, 1695, 1742
BseLI	CCNNNNNNNGG	3 cut(s)	772, 843, 1144
BseMI	GCAATG	3 cut(s)	483, 1089, 1291
BseMII	CTCAG	2 cut(s)	1376, 1733
BseNI	ACTGG	6 cut(s)	199, 244, 853, 1264, 1342, 1396
BseRI	GAGGAG	2 cut(s)	154, 753
BseSI	GKGCMC	2 cut(s)	891, 1415
BseXI	GCAGC	5 cut(s)	783, 786, 1114, 1301, 1682
BseYI	CCCAGC	1 cut(s)	771
Bsh1236I	CGCG	1 cut(s)	821
BshFI	GGCC	5 cut(s)	113, 350, 665, 836, 1648
BshNI	GGYRCC	2 cut(s)	125, 1262
BsiHKAI	GWGCWC	3 cut(s)	39, 968, 1415
BsiHKCI	CYCGRG	5 cut(s)	265, 728, 758, 766, 781
BsiSI	CCGG	7 cut(s)	135, 305, 492, 710, 718, 767, 782
BsiWI	CGTACG	1 cut(s)	936
BslFI	GGGAC	4 cut(s)	310, 859, 946, 1054
BslI	CCNNNNNNNGG	3 cut(s)	772, 843, 1144
BsmAI	GTCTC	5 cut(s)	62, 97, 422, 560, 780
BsmBI	CGTCTC	2 cut(s)	62, 97
BsmFI	GGGAC	4 cut(s)	310, 859, 946, 1054
BsmI	GAATGC	3 cut(s)	886, 1148, 1199
BsnI	GGCC	5 cut(s)	113, 350, 665, 836, 1648
Bso31I	GGTCTC	2 cut(s)	422, 560
BsoBI	CYCGRG	5 cut(s)	265, 728, 758, 766, 781
Bsp1286I	GDGCHC	4 cut(s)	39, 891, 968, 1415
Bsp143I	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
BspACI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 819, 1142
BspANI	GGCC	5 cut(s)	113, 350, 665, 836, 1648
BspCNI	CTCAG	2 cut(s)	1375, 1732
BspFNI	CGCG	1 cut(s)	821
BspLI	GGNNCC	5 cut(s)	127, 1162, 1264, 1377, 1446
BspMAI	CTGCAG	1 cut(s)	804
BspMI	ACCTGC	1 cut(s)	578
BspPI	GGATC	4 cut(s)	548, 741, 1553, 1615
BspQI	GCTCTTC	1 cut(s)	36
BspT107I	GGYRCC	2 cut(s)	125, 1262
BspTNI	GGTCTC	2 cut(s)	422, 560
BsrDI	GCAATG	3 cut(s)	483, 1089, 1291
BsrFI	RCCGGY	1 cut(s)	134
BsrI	ACTGG	6 cut(s)	199, 244, 853, 1264, 1342, 1396
BssAI	RCCGGY	1 cut(s)	134
BssECI	CCNNGG	8 cut(s)	510, 766, 781, 837, 1322, 1466, 1485, 1699
BssMI	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
BssNI	GRCGYC	2 cut(s)	126, 237
BssSI	CACGAG	1 cut(s)	256
BssT1I	CCWWGG	3 cut(s)	837, 1466, 1699
Bst2BI	CACGAG	1 cut(s)	256
Bst2UI	CCWGG	5 cut(s)	575, 662, 907, 1205, 1605
Bst4CI	ACNGT	7 cut(s)	91, 142, 433, 1250, 1310, 1489, 1684
Bst6I	CTCTTC	4 cut(s)	36, 172, 280, 1075
BstACI	GRCGYC	2 cut(s)	126, 237
BstAPI	GCANNNNNTGC	1 cut(s)	1333
BstBAI	YACGTR	1 cut(s)	826
BstC8I	GCNNGC	4 cut(s)	48, 71, 77, 652
BstDEI	CTNAG	3 cut(s)	1362, 1495, 1719
BstDSI	CCRYGG	1 cut(s)	1485
BstF5I	GGATG	7 cut(s)	144, 487, 628, 847, 1042, 1695, 1742
BstFNI	CGCG	1 cut(s)	821
BstH2I	RGCGCY	5 cut(s)	76, 129, 314, 414, 549
BstHHI	GCGC	6 cut(s)	75, 128, 313, 413, 548, 823
BstKTI	GATC	8 cut(s)	387, 522, 543, 749, 954, 1157, 1561, 1610
BstMAI	GTCTC	5 cut(s)	62, 97, 422, 560, 780
BstMBI	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
BstNI	CCWGG	5 cut(s)	575, 662, 907, 1205, 1605
BstNSI	RCATGY	1 cut(s)	335
BstSFI	CTRYAG	3 cut(s)	800, 1071, 1180
BstSLI	GKGCMC	2 cut(s)	891, 1415
BstUI	CGCG	1 cut(s)	821
BstV1I	GCAGC	5 cut(s)	783, 786, 1114, 1301, 1682
BstV2I	GAAGAC	2 cut(s)	539, 594
BstX2I	RGATCY	1 cut(s)	746
BstXI	CCANNNNNNTGG	2 cut(s)	1533, 1598
BstYI	RGATCY	1 cut(s)	746
BsuRI	GGCC	5 cut(s)	113, 350, 665, 836, 1648
BtgI	CCRYGG	1 cut(s)	1485
BtrI	CACGTC	1 cut(s)	537
BtsCI	GGATG	7 cut(s)	144, 487, 628, 847, 1042, 1695, 1742
BtsI	GCAGTG	2 cut(s)	1116, 1356
BtsIMutI	CAGTG	4 cut(s)	1116, 1257, 1356, 1389
BveI	ACCTGC	1 cut(s)	578
Cac8I	GCNNGC	4 cut(s)	48, 71, 77, 652
CaiI	CAGNNNCTG	2 cut(s)	814, 1664
CfoI	GCGC	6 cut(s)	75, 128, 313, 413, 548, 823
Cfr10I	RCCGGY	1 cut(s)	134
Cfr13I	GGNCC	3 cut(s)	1375, 1601, 1647
Cfr9I	CCCGGG	2 cut(s)	766, 781
CseI	GACGC	1 cut(s)	226
CsiI	ACCWGGT	1 cut(s)	905
Csp6I	GTAC	5 cut(s)	247, 733, 755, 937, 1263
CviAII	CATG	7 cut(s)	332, 553, 955, 969, 1276, 1438, 1632
CviQI	GTAC	5 cut(s)	247, 733, 755, 937, 1263
DdeI	CTNAG	3 cut(s)	1362, 1495, 1719
DinI	GGCGCC	1 cut(s)	127
DpnI	GATC	8 cut(s)	386, 521, 542, 748, 953, 1156, 1560, 1609
DpnII	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
EaeI	YGGCCR	3 cut(s)	111, 663, 834
Eam1104I	CTCTTC	4 cut(s)	36, 172, 280, 1075
EarI	CTCTTC	4 cut(s)	36, 172, 280, 1075
Ecl136II	GAGCTC	1 cut(s)	37
Eco130I	CCWWGG	3 cut(s)	837, 1466, 1699
Eco147I	AGGCCT	1 cut(s)	350
Eco24I	GRGCYC	1 cut(s)	39
Eco31I	GGTCTC	2 cut(s)	422, 560
Eco47I	GGWCC	2 cut(s)	1375, 1601
Eco47III	AGCGCT	3 cut(s)	74, 312, 412
Eco53kI	GAGCTC	1 cut(s)	37
Eco57I	CTGAAG	2 cut(s)	551, 1686
Eco72I	CACGTG	1 cut(s)	826
Eco88I	CYCGRG	5 cut(s)	265, 728, 758, 766, 781
EcoICRI	GAGCTC	1 cut(s)	37
EcoO109I	RGGNCCY	2 cut(s)	1375, 1647
EcoRI	GAATTC	1 cut(s)	614
EcoRII	CCWGG	5 cut(s)	573, 660, 905, 1203, 1603
EcoT14I	CCWWGG	3 cut(s)	837, 1466, 1699
EcoT22I	ATGCAT	1 cut(s)	1201
EcoT38I	GRGCYC	1 cut(s)	39
EgeI	GGCGCC	1 cut(s)	127
EheI	GGCGCC	1 cut(s)	127
ErhI	CCWWGG	3 cut(s)	837, 1466, 1699
Esp3I	CGTCTC	2 cut(s)	62, 97
FaeI	CATG	7 cut(s)	335, 556, 958, 972, 1279, 1441, 1635
FalI	AAGNNNNNCTT	2 cut(s)	884, 916
FaqI	GGGAC	4 cut(s)	310, 859, 946, 1054
FatI	CATG	7 cut(s)	331, 552, 954, 968, 1275, 1437, 1631
FauI	CCCGC	2 cut(s)	74, 199
FbaI	TGATCA	1 cut(s)	951
FblI	GTMKAC	2 cut(s)	30, 1462
Fnu4HI	GCNGC	6 cut(s)	235, 797, 800, 1128, 1290, 1671
FokI	GGATG	7 cut(s)	131, 494, 635, 854, 1049, 1682, 1729
FriOI	GRGCYC	1 cut(s)	39
Fsp4HI	GCNGC	6 cut(s)	235, 797, 800, 1128, 1290, 1671
GlaI	GCGC	6 cut(s)	74, 127, 312, 412, 547, 822
GluI	GCNGC	6 cut(s)	235, 797, 800, 1128, 1290, 1671
GsaI	CCCAGC	1 cut(s)	775
GsuI	CTGGAG	1 cut(s)	1143
HaeII	RGCGCY	5 cut(s)	76, 129, 314, 414, 549
HaeIII	GGCC	5 cut(s)	113, 350, 665, 836, 1648
HapII	CCGG	7 cut(s)	135, 305, 492, 710, 718, 767, 782
HgaI	GACGC	1 cut(s)	226
HhaI	GCGC	6 cut(s)	75, 128, 313, 413, 548, 823
Hin1I	GRCGYC	2 cut(s)	126, 237
Hin1II	CATG	7 cut(s)	335, 556, 958, 972, 1279, 1441, 1635
Hin6I	GCGC	6 cut(s)	73, 126, 311, 411, 546, 821
HinP1I	GCGC	6 cut(s)	73, 126, 311, 411, 546, 821
HincII	GTYRAC	2 cut(s)	31, 204
HindII	GTYRAC	2 cut(s)	31, 204
HindIII	AAGCTT	1 cut(s)	455
HinfI	GANTC	8 cut(s)	205, 464, 578, 1499, 1511, 1546, 1688, 1739
HpaII	CCGG	7 cut(s)	135, 305, 492, 710, 718, 767, 782
HphI	GGTGA	4 cut(s)	79, 124, 232, 707
Hpy166II	GTNNAC	8 cut(s)	31, 204, 715, 1413, 1463, 1536, 1601, 1680
Hpy188I	TCNGA	6 cut(s)	389, 511, 1154, 1165, 1407, 1722
Hpy188III	TCNNGA	7 cut(s)	265, 584, 611, 869, 1244, 1303, 1519
Hpy8I	GTNNAC	8 cut(s)	31, 204, 715, 1413, 1463, 1536, 1601, 1680
Hpy99I	CGWCG	2 cut(s)	35, 178
HpyAV	CCTTC	4 cut(s)	61, 381, 1366, 1702
HpyCH4III	ACNGT	7 cut(s)	91, 142, 433, 1250, 1310, 1489, 1684
HpyCH4IV	ACGT	4 cut(s)	536, 606, 825, 935
HpyCH4V	TGCA	9 cut(s)	331, 476, 631, 802, 1046, 1130, 1199, 1289, 1413
HpyF3I	CTNAG	3 cut(s)	1362, 1495, 1719
HpySE526I	ACGT	4 cut(s)	536, 606, 825, 935
Hsp92I	GRCGYC	2 cut(s)	126, 237
Hsp92II	CATG	7 cut(s)	335, 556, 958, 972, 1279, 1441, 1635
HspAI	GCGC	6 cut(s)	73, 126, 311, 411, 546, 821
KasI	GGCGCC	1 cut(s)	125
KpnI	GGTACC	1 cut(s)	1266
Ksp22I	TGATCA	1 cut(s)	951
Kzo9I	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
LguI	GCTCTTC	1 cut(s)	36
LmnI	GCTCC	4 cut(s)	167, 805, 1124, 1450
Lsp1109I	GCAGC	5 cut(s)	783, 786, 1114, 1301, 1682
LweI	GCATC	5 cut(s)	279, 433, 613, 1175, 1186
MabI	ACCWGGT	1 cut(s)	905
MaeII	ACGT	4 cut(s)	536, 606, 825, 935
MaeIII	GTNAC	8 cut(s)	85, 130, 238, 1244, 1250, 1310, 1627, 1640
MalI	GATC	8 cut(s)	386, 521, 542, 748, 953, 1156, 1560, 1609
MboI	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
MflI	RGATCY	1 cut(s)	746
MhlI	GDGCHC	4 cut(s)	39, 891, 968, 1415
MlsI	TGGCCA	2 cut(s)	665, 836
MluCI	AATT	7 cut(s)	595, 614, 678, 697, 1235, 1401, 1732
MluNI	TGGCCA	2 cut(s)	665, 836
Mly113I	GGCGCC	1 cut(s)	126
MlyI	GAGTC	3 cut(s)	199, 572, 1697
MmeI	TCCRAC	3 cut(s)	315, 1188, 1472
Mox20I	TGGCCA	2 cut(s)	665, 836
Mph1103I	ATGCAT	1 cut(s)	1201
MroXI	GAANNNNTTC	1 cut(s)	1545
MscI	TGGCCA	2 cut(s)	665, 836
MseI	TTAA	7 cut(s)	12, 221, 342, 417, 459, 858, 1113
MslI	CAYNNNNRTG	2 cut(s)	1531, 1747
Msp20I	TGGCCA	2 cut(s)	665, 836
MspA1I	CMGCKG	1 cut(s)	1292
MspI	CCGG	7 cut(s)	135, 305, 492, 710, 718, 767, 782
Mva1269I	GAATGC	3 cut(s)	886, 1148, 1199
MvaI	CCWGG	5 cut(s)	575, 662, 907, 1205, 1605
MvnI	CGCG	1 cut(s)	821
NarI	GGCGCC	1 cut(s)	126
NciI	CCSGG	5 cut(s)	305, 767, 768, 782, 783
NdeII	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
NlaIII	CATG	7 cut(s)	335, 556, 958, 972, 1279, 1441, 1635
NlaIV	GGNNCC	5 cut(s)	127, 1162, 1264, 1377, 1446
NmeAIII	GCCGAG	1 cut(s)	1347
NmuCI	GTSAC	5 cut(s)	85, 130, 238, 1244, 1250
NsiI	ATGCAT	1 cut(s)	1201
NspI	RCATGY	1 cut(s)	335
PaeR7I	CTCGAG	1 cut(s)	265
PceI	AGGCCT	1 cut(s)	350
PciSI	GCTCTTC	1 cut(s)	36
PcsI	WCGNNNNNNNCGW	5 cut(s)	27, 36, 176, 263, 342
PctI	GAATGC	3 cut(s)	886, 1148, 1199
PdmI	GAANNNNTTC	1 cut(s)	1545
PfeI	GAWTC	5 cut(s)	464, 1499, 1511, 1546, 1739
Pfl23II	CGTACG	1 cut(s)	936
PflMI	CCANNNNNTGG	1 cut(s)	1144
PfoI	TCCNGGA	1 cut(s)	1203
PkrI	GCNGC	6 cut(s)	236, 798, 801, 1129, 1291, 1672
PleI	GAGTC	3 cut(s)	199, 572, 1696
PluTI	GGCGCC	1 cut(s)	129
PmaCI	CACGTG	1 cut(s)	826
PmlI	CACGTG	1 cut(s)	826
PpsI	GAGTC	3 cut(s)	199, 572, 1696
Ppu21I	YACGTR	1 cut(s)	826
PpuMI	RGGWCCY	1 cut(s)	1375
Psp124BI	GAGCTC	1 cut(s)	39
Psp5II	RGGWCCY	1 cut(s)	1375
Psp6I	CCWGG	5 cut(s)	573, 660, 905, 1203, 1603
PspCI	CACGTG	1 cut(s)	826
PspFI	CCCAGC	1 cut(s)	771
PspGI	CCWGG	5 cut(s)	573, 660, 905, 1203, 1603
PspLI	CGTACG	1 cut(s)	936
PspN4I	GGNNCC	5 cut(s)	127, 1162, 1264, 1377, 1446
PspPI	GGNCC	3 cut(s)	1375, 1601, 1647
PspPPI	RGGWCCY	1 cut(s)	1375
PstI	CTGCAG	1 cut(s)	804
PstNI	CAGNNNCTG	2 cut(s)	814, 1664
PsuI	RGATCY	1 cut(s)	746
PvuII	CAGCTG	1 cut(s)	1292
RsaI	GTAC	5 cut(s)	248, 734, 756, 938, 1264
RsaNI	GTAC	5 cut(s)	247, 733, 755, 937, 1263
RseI	CAYNNNNRTG	2 cut(s)	1531, 1747
SacI	GAGCTC	1 cut(s)	39
SalI	GTCGAC	1 cut(s)	29
SapI	GCTCTTC	1 cut(s)	36
SaqAI	TTAA	7 cut(s)	12, 221, 342, 417, 459, 858, 1113
SatI	GCNGC	6 cut(s)	235, 797, 800, 1128, 1290, 1671
Sau3AI	GATC	8 cut(s)	384, 519, 540, 746, 951, 1154, 1558, 1607
Sau96I	GGNCC	3 cut(s)	1375, 1601, 1647
SchI	GAGTC	3 cut(s)	199, 572, 1697
SduI	GDGCHC	4 cut(s)	39, 891, 968, 1415
SexAI	ACCWGGT	1 cut(s)	905
SfaNI	GCATC	5 cut(s)	279, 433, 613, 1175, 1186
SfcI	CTRYAG	3 cut(s)	800, 1071, 1180
SfoI	GGCGCC	1 cut(s)	127
Sfr274I	CTCGAG	1 cut(s)	265
SinI	GGWCC	2 cut(s)	1375, 1601
SlaI	CTCGAG	1 cut(s)	265
SmaI	CCCGGG	2 cut(s)	768, 783
SmiMI	CAYNNNNRTG	2 cut(s)	1531, 1747
SmlI	CTYRAG	1 cut(s)	265
SmoI	CTYRAG	1 cut(s)	265
Sse9I	AATT	7 cut(s)	595, 614, 678, 697, 1235, 1401, 1732
SseBI	AGGCCT	1 cut(s)	350
SsiI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 819, 1142
SspDI	GGCGCC	1 cut(s)	125
SspI	AATATT	1 cut(s)	1507
SstI	GAGCTC	1 cut(s)	39
StuI	AGGCCT	1 cut(s)	350
StyI	CCWWGG	3 cut(s)	837, 1466, 1699
TaaI	ACNGT	7 cut(s)	91, 142, 433, 1250, 1310, 1489, 1684
TaiI	ACGT	4 cut(s)	539, 609, 828, 938
TasI	AATT	7 cut(s)	595, 614, 678, 697, 1235, 1401, 1732
TauI	GCSGC	1 cut(s)	237
TfiI	GAWTC	5 cut(s)	464, 1499, 1511, 1546, 1739
Tru1I	TTAA	7 cut(s)	12, 221, 342, 417, 459, 858, 1113
Tru9I	TTAA	7 cut(s)	12, 221, 342, 417, 459, 858, 1113
TscAI	CASTG	4 cut(s)	1123, 1264, 1363, 1396
TseFI	GTSAC	5 cut(s)	85, 130, 238, 1244, 1250
TseI	GCWGC	5 cut(s)	796, 799, 1127, 1289, 1670
Tsp45I	GTSAC	5 cut(s)	85, 130, 238, 1244, 1250
TspDTI	ATGAA	6 cut(s)	1078, 1099, 1264, 1684, 1722, 1731
TspGWI	ACGGA	3 cut(s)	44, 85, 750
TspMI	CCCGGG	2 cut(s)	766, 781
TspRI	CASTG	4 cut(s)	1123, 1264, 1363, 1396
Van91I	CCANNNNNTGG	1 cut(s)	1144
VneI	GTGCAC	1 cut(s)	1411
VpaK11BI	GGWCC	2 cut(s)	1375, 1601
XapI	RAATTY	3 cut(s)	614, 678, 1732
XceI	RCATGY	1 cut(s)	335
XcmI	CCANNNNNNNNNTGG	1 cut(s)	658
XhoI	CTCGAG	1 cut(s)	265
XmaI	CCCGGG	2 cut(s)	766, 781
XmiI	GTMKAC	2 cut(s)	30, 1462
XmnI	GAANNNNTTC	1 cut(s)	1545
Zsp2I	ATGCAT	1 cut(s)	1201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.