RchiOBHm_Chr1g0326461
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012516
Physical Location & Seq
Forward (+)
15022163 .. 15025412
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
ATGCAAGTATTTACCGTTTGGATTTTTTTAATAAAAACTTGTAGGGTTTGTGAAGGGTTTAAGCCGTTTCTAACTTTCTATCTCTCACCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAAATCAGTCATGGCGAACCGTACGGAATACGACCCGACCTTGACAGACGACGAAGACGAAGTTGAGGAGCTCCAAGACTGGGACGATTGGAACGCCGAAGAAGAAGAAGAAGAAGAGAGCTCCGACTTGGACTCAGACTTTCTCTGCTTCTTCTGCCACTCCAGGTTCAGTTCCTGCGACGAACTGTTCGACCACTGCAGCGCCACTCACCATTTCGATTTTCCCGCCATTCGGAAAGCCCTGGGCTTATTAACTACCTTCGCTCTCAGCAATGTTGCGGATAACAGATGTTGGAGTTGCGGGCTAAGCTGTCAGTCCAACAAAGATTTACAAAGTCATTTACATGAAACAGTCAATTTGAAAGATATTAAACCTATGTGGAATAGCGATGAGTATCTCAAACCTTTCTTGCAAGATGATTCAGTCTTGTTTAGTTTCGGTGAAGATGAAGAAGGCGACGATGACTACACTGTAGCGGTTGACAAGGATGAACTTATGAGTGATTTGCGGCATTTTGAAGAGATTTGCATTGATCATCAGATTCATGTGGAAAAGATTGCTGGTGATTCGGATAACTCTTGTGAGAGTGGGAAAAGAGTTGTTGATTCATCATCGAATGGCTACTTGAATACAGCTAGCTCATCTGGAAATGTGACTGCTAATGGTGTGAATTCAGTAGAGCATGTTGTGTCAGCTGATAGAAAGACAAAAGGAAAGAGTATAAAAGCATATATTCCAAATCATGTTTCAAAGGATATGAAGAATATCAATGACAATTACTTTGCCTCTTACAGCTCATATGGCATTCACAGAGAAATGCTCAGTGATAAGAATGGATGCTTATGGACAGTCTATTTTAAAGAATCCCTCCCTCTTGAAGAGCGCATAGTGATGGATGTAGGTTGTGGCACAGGCATACTTAGTCTCTTTGCAGCACAAGCAGGGGCTTCAAGGGTAATTGCAGTTGAAGCAAGTGAGAAGATGGCTTCAGTGGCAACTCTGATAGCAAAAGATAATGGTCTTTTGTGGAAGAGTCCAGTTGGCAGTACTGCCAATGGCACTGGGGTAATAGAAGTGGTTCAAGGTATGGTTGAGGAGCTTGATAAATCCATGGTGATCCAACCTCATAGTGTTGATGTATTACTGAGTGAATGGATGGGGTATTGCCTACTTTATGAGGCAATGCTCAGTTCAGTGCTCTTTGACGGGATAAGTGGTTGA

Protein Analysis

445

Amino Acids

49.6

Weight (kDa)

4.47

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_2 PF12756 88 - 155 2.6e-06 C2H2 type zinc-finger (2 copies)
PrmA PF06325 327 - 378 1.1e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_25 PF13649 336 - 380 9.9e-07 Methyltransferase domain
Methyltransf_11 PF08241 338 - 424 6.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012516)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12270
fragaria_vesca FvH4_7g03650
malus_domestica MD07G1046100.v1.1
prunus_persica Prupe.2G042600_v2.0.a1
pyrus_communis pycom07g03320
rosa_chinensis RchiOBHm_Chr1g0326421 RchiOBHm_Chr1g0326461 RchiOBHm_Chr1g0326471
rosa_laevigata RLG00000030070
rosa_multiflora Rmu_sc0002687.1_g000007 Rmu_sc0006561.1_g000035
rosa_roxburghii Rroxscaffold_4G00323720 Rroxscaffold_4G00323770
rosa_rugosa Rorug01G0060400
rosa_samantha Rh1AG076500 Rh1BG062400 Rh1CG074600 Rh1DG081900
rosa_wichuraiana Rw1G006100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 342, 395, 417, 593, 625
AclWI GGATC 1 cut(s) 1228
AcsI RAATTY 1 cut(s) 787
AcuI CTGAAG 1 cut(s) 1089
AfaI GTAC 2 cut(s) 139, 1165
AfiI CCNNNNNNNGG 2 cut(s) 196, 348
AgsI TTSAA 8 cut(s) 478, 635, 745, 867, 995, 1068, 1085, 1199
AjnI CCWGG 2 cut(s) 278, 357
AjuI GAANNNNNNNTTGG 2 cut(s) 847, 879
AluBI AGCT 8 cut(s) 187, 237, 426, 752, 756, 812, 912, 1216
AluI AGCT 8 cut(s) 187, 237, 426, 752, 756, 812, 912, 1216
Alw21I GWGCWC 3 cut(s) 189, 239, 1317
Alw26I GTCTC 1 cut(s) 1046
AlwI GGATC 1 cut(s) 1228
AlwNI CAGNNNCTG 1 cut(s) 291
ApeKI GCWGC 2 cut(s) 315, 1049
ApoI RAATTY 1 cut(s) 787
ArsI GACNNNNNNTTYG 4 cut(s) 287, 319, 881, 913
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 2 cut(s) 320, 1002
AsuHPI GGTGA 5 cut(s) 78, 317, 569, 692, 1243
AsuNHI GCTAGC 1 cut(s) 752
BanII GRGCYC 2 cut(s) 189, 239
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 3 cut(s) 189, 239, 1317
BbvI GCAGC 2 cut(s) 327, 1061
BccI CCATC 3 cut(s) 1003, 1093, 1267
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 2 cut(s) 280, 359
BclI TGATCA 1 cut(s) 649
BcoDI GTCTC 1 cut(s) 1046
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 2 cut(s) 313, 588
BfoI RGCGCY 1 cut(s) 321
BisI GCNGC 3 cut(s) 316, 626, 1050
BlpI GCTNAGC 1 cut(s) 422
BlsI GCNGC 3 cut(s) 317, 627, 1051
BmcAI AGTACT 1 cut(s) 1165
Bme1390I CCNGG 2 cut(s) 280, 359
BmrFI CCNGG 2 cut(s) 280, 359
BmrI ACTGGG 2 cut(s) 205, 1188
BmsI GCATC 1 cut(s) 944
BmtI GCTAGC 1 cut(s) 756
BmuI ACTGGG 2 cut(s) 205, 1188
BpiI GAAGAC 1 cut(s) 177
BplI GAGNNNNNCTC 2 cut(s) 921, 953
BpmI CTGGAG 1 cut(s) 262
Bpu1102I GCTNAGC 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 510
BsaJI CCNNGG 3 cut(s) 357, 358, 1227
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 348
Bse1I ACTGG 3 cut(s) 200, 1154, 1183
Bse3DI GCAATG 2 cut(s) 394, 1305
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 2 cut(s) 280, 359
BseDI CCNNGG 3 cut(s) 357, 358, 1227
BseGI GGATG 4 cut(s) 610, 959, 1018, 1278
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 196, 348
BseMI GCAATG 2 cut(s) 394, 1305
BseMII CTCAG 5 cut(s) 264, 397, 952, 1253, 1318
BseNI ACTGG 3 cut(s) 200, 1154, 1183
BseRI GAGGAG 2 cut(s) 197, 1226
BseXI GCAGC 2 cut(s) 327, 1061
BsiHKAI GWGCWC 3 cut(s) 189, 239, 1317
BsiWI CGTACG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 196, 348
BsmAI GTCTC 1 cut(s) 1046
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 921
Bsp1286I GDGCHC 3 cut(s) 189, 239, 1317
Bsp143I GATC 2 cut(s) 649, 1233
Bsp1720I GCTNAGC 1 cut(s) 422
Bsp19I CCATGG 1 cut(s) 1227
BspACI CCGC 5 cut(s) 342, 395, 417, 593, 625
BspCNI CTCAG 5 cut(s) 263, 396, 951, 1254, 1317
BspMAI CTGCAG 1 cut(s) 317
BspOI GCTAGC 1 cut(s) 756
BspPI GGATC 1 cut(s) 1228
BspQI GCTCTTC 1 cut(s) 990
BsrDI GCAATG 2 cut(s) 394, 1305
BsrI ACTGG 3 cut(s) 200, 1154, 1183
BssECI CCNNGG 3 cut(s) 357, 358, 1227
BssMI GATC 2 cut(s) 649, 1233
BssT1I CCWWGG 1 cut(s) 1227
Bst2UI CCWGG 2 cut(s) 280, 359
Bst4CI ACNGT 6 cut(s) 16, 137, 303, 469, 589, 967
Bst6I CTCTTC 4 cut(s) 225, 630, 990, 1142
BstC8I GCNNGC 2 cut(s) 419, 754
BstDEI CTNAG 7 cut(s) 250, 383, 422, 938, 1037, 1262, 1304
BstDSI CCRYGG 1 cut(s) 1227
BstF5I GGATG 4 cut(s) 610, 959, 1018, 1278
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 2 cut(s) 320, 1002
BstKTI GATC 2 cut(s) 652, 1236
BstMAI GTCTC 1 cut(s) 1046
BstMBI GATC 2 cut(s) 649, 1233
BstMWI GCNNNNNNNGC 6 cut(s) 270, 423, 918, 1055, 1085, 1109
BstNI CCWGG 2 cut(s) 280, 359
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 2 cut(s) 278, 357
BstSFI CTRYAG 2 cut(s) 313, 588
BstV1I GCAGC 2 cut(s) 327, 1061
BstV2I GAAGAC 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 1227
BtgZI GCGATG 1 cut(s) 519
BtsCI GGATG 4 cut(s) 610, 959, 1018, 1278
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 6 cut(s) 310, 585, 946, 1113, 1176, 1317
Cac8I GCNNGC 2 cut(s) 419, 754
CaiI CAGNNNCTG 1 cut(s) 291
CfoI GCGC 2 cut(s) 320, 1002
Csp6I GTAC 2 cut(s) 138, 1164
CviAII CATG 6 cut(s) 127, 461, 662, 800, 860, 1228
CviQI GTAC 2 cut(s) 138, 1164
DdeI CTNAG 7 cut(s) 250, 383, 422, 938, 1037, 1262, 1304
DpnI GATC 2 cut(s) 651, 1235
DpnII GATC 2 cut(s) 649, 1233
DraI TTTAAA 1 cut(s) 976
Eam1104I CTCTTC 4 cut(s) 225, 630, 990, 1142
EarI CTCTTC 4 cut(s) 225, 630, 990, 1142
Ecl136II GAGCTC 2 cut(s) 187, 237
Eco130I CCWWGG 1 cut(s) 1227
Eco24I GRGCYC 2 cut(s) 189, 239
Eco53kI GAGCTC 2 cut(s) 187, 237
Eco57I CTGAAG 1 cut(s) 1089
EcoICRI GAGCTC 2 cut(s) 187, 237
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 2 cut(s) 278, 357
EcoT14I CCWWGG 1 cut(s) 1227
EcoT38I GRGCYC 2 cut(s) 189, 239
ErhI CCWWGG 1 cut(s) 1227
FaeI CATG 6 cut(s) 130, 464, 665, 803, 863, 1231
FaqI GGGAC 1 cut(s) 212
FatI CATG 6 cut(s) 126, 460, 661, 799, 859, 1227
FauI CCCGC 2 cut(s) 349, 410
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 3 cut(s) 316, 626, 1050
FokI GGATG 4 cut(s) 617, 966, 1025, 1285
FriOI GRGCYC 2 cut(s) 189, 239
Fsp4HI GCNGC 3 cut(s) 316, 626, 1050
FspBI CTAG 1 cut(s) 753
GlaI GCGC 2 cut(s) 319, 1001
GluI GCNGC 3 cut(s) 316, 626, 1050
GsuI CTGGAG 1 cut(s) 262
HaeII RGCGCY 1 cut(s) 321
HhaI GCGC 2 cut(s) 320, 1002
Hin1II CATG 6 cut(s) 130, 464, 665, 803, 863, 1231
Hin6I GCGC 2 cut(s) 318, 1000
HinP1I GCGC 2 cut(s) 318, 1000
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 7 cut(s) 248, 536, 658, 683, 722, 980, 1150
HphI GGTGA 5 cut(s) 78, 317, 569, 692, 1243
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 6 cut(s) 241, 253, 351, 657, 688, 1119
Hpy188III TCNNGA 2 cut(s) 762, 992
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 3 cut(s) 170, 299, 578
HpyAV CCTTC 3 cut(s) 47, 385, 563
HpyCH4III ACNGT 6 cut(s) 16, 137, 303, 469, 589, 967
HpyCH4V TGCA 6 cut(s) 4, 315, 529, 645, 1049, 1079
HpyF10VI GCNNNNNNNGC 6 cut(s) 270, 423, 918, 1055, 1085, 1109
HpyF3I CTNAG 7 cut(s) 250, 383, 422, 938, 1037, 1262, 1304
Hsp92II CATG 6 cut(s) 130, 464, 665, 803, 863, 1231
HspAI GCGC 2 cut(s) 318, 1000
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 2 cut(s) 649, 1233
LguI GCTCTTC 1 cut(s) 990
LmnI GCTCC 4 cut(s) 184, 192, 242, 1213
Lsp1109I GCAGC 2 cut(s) 327, 1061
LweI GCATC 1 cut(s) 944
MaeI CTAG 1 cut(s) 753
MaeIII GTNAC 1 cut(s) 769
MalI GATC 2 cut(s) 651, 1235
MboI GATC 2 cut(s) 649, 1233
MhlI GDGCHC 3 cut(s) 189, 239, 1317
MluCI AATT 4 cut(s) 472, 787, 892, 1074
MlyI GAGTC 2 cut(s) 242, 1159
MmeI TCCRAC 4 cut(s) 264, 389, 459, 1261
MnlI CCTC 8 cut(s) 99, 175, 913, 995, 999, 1204, 1251, 1288
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 5 cut(s) 29, 60, 368, 486, 975
MslI CAYNNNNRTG 2 cut(s) 459, 1007
MspA1I CMGCKG 1 cut(s) 812
MspR9I CCNGG 2 cut(s) 280, 359
Mva1269I GAATGC 1 cut(s) 921
MvaI CCWGG 2 cut(s) 280, 359
MwoI GCNNNNNNNGC 6 cut(s) 270, 423, 918, 1055, 1085, 1109
NcoI CCATGG 1 cut(s) 1227
NdeI CATATG 1 cut(s) 916
NdeII GATC 2 cut(s) 649, 1233
NheI GCTAGC 1 cut(s) 752
NlaIII CATG 6 cut(s) 130, 464, 665, 803, 863, 1231
NmuCI GTSAC 1 cut(s) 769
NspI RCATGY 1 cut(s) 803
PasI CCCWGGG 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 990
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 303
PctI GAATGC 1 cut(s) 921
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 5 cut(s) 536, 658, 683, 722, 980
Pfl23II CGTACG 1 cut(s) 137
PkrI GCNGC 3 cut(s) 317, 627, 1051
PleI GAGTC 2 cut(s) 242, 1158
PpsI GAGTC 2 cut(s) 242, 1158
Psp124BI GAGCTC 2 cut(s) 189, 239
Psp6I CCWGG 2 cut(s) 278, 357
PspGI CCWGG 2 cut(s) 278, 357
PspLI CGTACG 1 cut(s) 137
PstI CTGCAG 1 cut(s) 317
PstNI CAGNNNCTG 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 2 cut(s) 139, 1165
RsaNI GTAC 2 cut(s) 138, 1164
RseI CAYNNNNRTG 2 cut(s) 459, 1007
SacI GAGCTC 2 cut(s) 189, 239
SapI GCTCTTC 1 cut(s) 990
SaqAI TTAA 5 cut(s) 29, 60, 368, 486, 975
SatI GCNGC 3 cut(s) 316, 626, 1050
Sau3AI GATC 2 cut(s) 649, 1233
ScaI AGTACT 1 cut(s) 1165
SchI GAGTC 2 cut(s) 242, 1159
ScrFI CCNGG 2 cut(s) 280, 359
SduI GDGCHC 3 cut(s) 189, 239, 1317
SfaNI GCATC 1 cut(s) 944
SfcI CTRYAG 2 cut(s) 313, 588
SmiMI CAYNNNNRTG 2 cut(s) 459, 1007
Sse9I AATT 4 cut(s) 472, 787, 892, 1074
SsiI CCGC 5 cut(s) 342, 395, 417, 593, 625
SspMI CTAG 1 cut(s) 753
SstI GAGCTC 2 cut(s) 189, 239
StyD4I CCNGG 2 cut(s) 278, 357
StyI CCWWGG 1 cut(s) 1227
TaaI ACNGT 6 cut(s) 16, 137, 303, 469, 589, 967
TaqI TCGA 3 cut(s) 306, 333, 731
TasI AATT 4 cut(s) 472, 787, 892, 1074
TatI WGTACW 1 cut(s) 1163
TauI GCSGC 1 cut(s) 628
TfiI GAWTC 5 cut(s) 536, 658, 683, 722, 980
Tru1I TTAA 5 cut(s) 29, 60, 368, 486, 975
Tru9I TTAA 5 cut(s) 29, 60, 368, 486, 975
TscAI CASTG 6 cut(s) 317, 592, 946, 1113, 1183, 1317
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 2 cut(s) 315, 1049
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 6 cut(s) 477, 579, 621, 650, 714, 890
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 6 cut(s) 317, 592, 946, 1113, 1183, 1317
XapI RAATTY 1 cut(s) 787
XceI RCATGY 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 753
ZrmI AGTACT 1 cut(s) 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.