RchiOBHm_Chr1g0346041

PRA1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008159
Physical Location & Seq
Reverse (-)
38202679 .. 38203535
857 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 594 bp
ATGGCTAGCCATGGCACCGTGCAAAGACCATCCACCACGTCAACATCCCCAACTCTCCCAATAGCTCATCCCCACGAACCGAAAGACTCAACACCAAGATTGAAACTCGAATTCAGCCTCTGTTTTCCATTCAACATCCCCAAGACTCCAGAAGCAGCAGCCGCCCGCATCATCAGAAACTTGAGTTACTACAGATTGTACTACACACTTTTCATATGGATCATACTCTTCACAACCCTCTTGCCGGAGCGCAAGCTCTCCTTGATCTTCTTGATATCAATGACTTCTTTGACCTGCTCGTTACTTGCAGTGATGAGATCAGTCGTTCCTGCTGATTCTGTTCTCCTGCAATACAAGATCATTGATAGGAGACTGCTTCTGGCTTTGCTGGTAATTGCGACCATGGTTGAGCTCATAGGCACACATGCGGCCATGCATCTGTATGTGACTTTGGCTTGTGCGACTCCAGTGATTTTGGTGCATGCATTTTTCAGGGTTAGAGATTATCTTTGTGTTGAAGAGGAGGCTTCTGCTGCTGGTGAATTGGTTCCACTCACAAAAAACACTGAACCCAAGTTATCAGATTCCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

197

Amino Acids

22.0

Weight (kDa)

8.71

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 44 - 174 5.7e-18 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 14
AciI CCGC 3 cut(s) 162, 166, 428
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 1 cut(s) 110
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 244
AgsI TTSAA 3 cut(s) 103, 133, 518
AjiI CACGTC 1 cut(s) 39
AluBI AGCT 3 cut(s) 65, 256, 412
AluI AGCT 3 cut(s) 65, 256, 412
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 1 cut(s) 364
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 120
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 3 cut(s) 155, 158, 533
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 551
AsuNHI GCTAGC 1 cut(s) 5
BanI GGYRCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 3 cut(s) 167, 170, 520
BccI CCATC 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 364
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 5 cut(s) 156, 159, 162, 429, 534
BlsI GCNGC 5 cut(s) 157, 160, 163, 430, 535
BmgBI CACGTC 1 cut(s) 39
BmiI GGNNCC 2 cut(s) 16, 549
BmsI GCATC 2 cut(s) 177, 445
BmtI GCTAGC 1 cut(s) 9
BpmI CTGGAG 2 cut(s) 132, 450
BpuEI CTTGAG 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 10, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 467
BseDI CCNNGG 2 cut(s) 10, 402
BseGI GGATG 4 cut(s) 29, 44, 67, 135
BseLI CCNNNNNNNGG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 3 cut(s) 167, 170, 520
BshFI GGCC 1 cut(s) 431
BshNI GGYRCC 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 1 cut(s) 364
BsnI GGCC 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 4 cut(s) 219, 264, 317, 357
Bsp19I CCATGG 2 cut(s) 10, 402
BspACI CCGC 3 cut(s) 162, 166, 428
BspANI GGCC 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 16, 549
BspMI ACCTGC 1 cut(s) 302
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 14
BsrI ACTGG 1 cut(s) 467
BssECI CCNNGG 2 cut(s) 10, 402
BssMI GATC 4 cut(s) 219, 264, 317, 357
BssT1I CCWWGG 2 cut(s) 10, 402
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 2 cut(s) 233, 513
BstC8I GCNNGC 4 cut(s) 7, 166, 254, 483
BstDSI CCRYGG 2 cut(s) 10, 402
BstF5I GGATG 4 cut(s) 29, 44, 67, 135
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 4 cut(s) 222, 267, 320, 360
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 4 cut(s) 219, 264, 317, 357
BstMWI GCNNNNNNNGC 2 cut(s) 161, 533
BstNSI RCATGY 2 cut(s) 428, 485
BstSFI CTRYAG 1 cut(s) 190
BstV1I GCAGC 3 cut(s) 167, 170, 520
BsuRI GGCC 1 cut(s) 431
BtgI CCRYGG 2 cut(s) 10, 402
BtrI CACGTC 1 cut(s) 39
BtsCI GGATG 4 cut(s) 29, 44, 67, 135
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 3 cut(s) 315, 474, 564
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 4 cut(s) 7, 166, 254, 483
CaiI CAGNNNCTG 1 cut(s) 120
CfoI GCGC 1 cut(s) 252
Csp6I GTAC 1 cut(s) 199
CviAII CATG 5 cut(s) 11, 403, 425, 433, 482
CviQI GTAC 1 cut(s) 199
DpnI GATC 4 cut(s) 221, 266, 319, 359
DpnII GATC 4 cut(s) 219, 264, 317, 357
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 2 cut(s) 233, 513
EarI CTCTTC 2 cut(s) 233, 513
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 2 cut(s) 10, 402
Eco24I GRGCYC 1 cut(s) 414
Eco32I GATATC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 110
EcoRV GATATC 1 cut(s) 276
EcoT14I CCWWGG 2 cut(s) 10, 402
EcoT22I ATGCAT 2 cut(s) 438, 487
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 2 cut(s) 10, 402
FaeI CATG 5 cut(s) 14, 406, 428, 436, 485
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FatI CATG 5 cut(s) 10, 402, 424, 432, 481
FauI CCCGC 1 cut(s) 173
FauNDI CATATG 1 cut(s) 215
Fnu4HI GCNGC 5 cut(s) 156, 159, 162, 429, 534
FokI GGATG 4 cut(s) 16, 31, 54, 122
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 5 cut(s) 156, 159, 162, 429, 534
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 251
GluI GCNGC 5 cut(s) 156, 159, 162, 429, 534
GsuI CTGGAG 2 cut(s) 132, 450
HaeIII GGCC 1 cut(s) 431
HapII CCGG 1 cut(s) 245
HhaI GCGC 1 cut(s) 252
Hin1II CATG 5 cut(s) 14, 406, 428, 436, 485
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HinfI GANTC 5 cut(s) 86, 145, 335, 463, 584
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 551
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 2 cut(s) 176, 583
Hpy188III TCNNGA 2 cut(s) 149, 271
Hpy8I GTNNAC 1 cut(s) 42
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 6 cut(s) 22, 308, 349, 436, 481, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 533
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 5 cut(s) 14, 406, 428, 436, 485
HspAI GCGC 1 cut(s) 250
Kzo9I GATC 4 cut(s) 219, 264, 317, 357
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 3 cut(s) 167, 170, 520
LweI GCATC 2 cut(s) 177, 445
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 3 cut(s) 185, 300, 445
MalI GATC 4 cut(s) 221, 266, 319, 359
MboI GATC 4 cut(s) 219, 264, 317, 357
MboII GAAGA 3 cut(s) 220, 259, 530
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 3 cut(s) 110, 393, 542
MlyI GAGTC 3 cut(s) 80, 139, 457
MnlI CCTC 4 cut(s) 128, 248, 514, 517
Mph1103I ATGCAT 2 cut(s) 438, 487
MroXI GAANNNNTTC 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 441
MspI CCGG 1 cut(s) 245
MwoI GCNNNNNNNGC 2 cut(s) 161, 533
NcoI CCATGG 2 cut(s) 10, 402
NdeI CATATG 1 cut(s) 215
NdeII GATC 4 cut(s) 219, 264, 317, 357
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 5 cut(s) 14, 406, 428, 436, 485
NlaIV GGNNCC 2 cut(s) 16, 549
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 2 cut(s) 438, 487
NspI RCATGY 2 cut(s) 428, 485
PaeI GCATGC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 2 cut(s) 335, 584
PkrI GCNGC 5 cut(s) 157, 160, 163, 430, 535
PleI GAGTC 3 cut(s) 80, 139, 457
PpsI GAGTC 3 cut(s) 80, 139, 457
Psp124BI GAGCTC 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 16, 549
PstNI CAGNNNCTG 1 cut(s) 120
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 441
SacI GAGCTC 1 cut(s) 414
SatI GCNGC 5 cut(s) 156, 159, 162, 429, 534
Sau3AI GATC 4 cut(s) 219, 264, 317, 357
SchI GAGTC 3 cut(s) 80, 139, 457
SduI GDGCHC 1 cut(s) 414
SetI ASST 5 cut(s) 41, 67, 258, 296, 414
SfaNI GCATC 2 cut(s) 177, 445
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
SphI GCATGC 1 cut(s) 485
Sse9I AATT 3 cut(s) 110, 393, 542
SsiI CCGC 3 cut(s) 162, 166, 428
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 414
StyI CCWWGG 2 cut(s) 10, 402
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 108
TasI AATT 3 cut(s) 110, 393, 542
TatI WGTACW 1 cut(s) 198
TauI GCSGC 2 cut(s) 164, 431
TfiI GAWTC 2 cut(s) 335, 584
TscAI CASTG 3 cut(s) 315, 474, 571
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 3 cut(s) 155, 158, 533
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 1 cut(s) 202
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 3 cut(s) 315, 474, 571
XapI RAATTY 1 cut(s) 110
XceI RCATGY 2 cut(s) 428, 485
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 2 cut(s) 438, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.