RchiOBHm_Chr1g0356311

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018552
Physical Location & Seq
Reverse (-)
48802785 .. 48803165
381 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGATATTAATTAGGAAAGATAGTATTTATATTTACTTTCAAGTTCTGAACCCTTTGTACTTGCATTACAAGTTAGGGTGGGATGAGGAGTTCAAGTCTGTGAAGGCAAACACTACGTTAGCATCAGCAATTCAGATTGAGGATCCTGTTTGGATTGACAGAGCTGTGTATGCGCTGAAGAAGTTGGATGGGAATGTAGAGGAGGTGACCAAGGAGGAGTTGATGGATGCAATGGCGCAAGTGGATTCTACTGGCATGTTTATATGTCCTCACACTGGTGTGGCGTTGGTGGCGTTGATTAAGCTCCGAAATAAGGGGGTTATAGGCCCTACTGAGAGGACTGTGGTGGTGAGCACGGCGCATGGGTTGAAGTTTACGTAG

Protein Analysis

126

Amino Acids

14.16

Weight (kDa)

6.28

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 32 - 119 1.4e-10 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018552)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0356311 RchiOBHm_Chr4g0415221
rosa_laevigata RLG00000009747
rosa_multiflora Rmu_co8170908.1_g000001 Rmu_co8374445.1_g000001
rosa_roxburghii Rroxscaffold_3G00228730 Rroxscaffold_5G00347460
rosa_rugosa Rorug03G0221500.1
rosa_samantha Rh2AG556000 Rh3DG267000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 137, 150
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 2 cut(s) 275, 313
AgsI TTSAA 3 cut(s) 41, 94, 370
AleI CACNNNNGTG 2 cut(s) 276, 278
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 2 cut(s) 164, 304
AluI AGCT 2 cut(s) 164, 304
Alw21I GWGCWC 1 cut(s) 356
AlwI GGATC 2 cut(s) 137, 150
AoxI GGCC 1 cut(s) 325
AseI ATTAAT 1 cut(s) 8
AspLEI GCGC 3 cut(s) 175, 238, 361
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 217, 361
BamHI GGATCC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 356
BccI CCATC 2 cut(s) 182, 217
BceAI ACGGC 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 1 cut(s) 144
BmsI GCATC 2 cut(s) 131, 217
BsaAI YACGTR 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 313
Bse1I ACTGG 2 cut(s) 256, 280
Bse3DI GCAATG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 3 cut(s) 88, 193, 232
BseLI CCNNNNNNNGG 2 cut(s) 275, 313
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 256, 280
BseRI GAGGAG 3 cut(s) 101, 215, 230
BshFI GGCC 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 356
BslI CCNNNNNNNGG 2 cut(s) 275, 313
BsnI GGCC 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 1 cut(s) 142
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 144
BspPI GGATC 2 cut(s) 137, 150
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 2 cut(s) 256, 280
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 378
BstDEI CTNAG 1 cut(s) 333
BstEII GGTNACC 1 cut(s) 205
BstF5I GGATG 3 cut(s) 88, 193, 232
BstHHI GCGC 3 cut(s) 175, 238, 361
BstKTI GATC 1 cut(s) 145
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 2 cut(s) 170, 290
BstNSI RCATGY 1 cut(s) 259
BstPI GGTNACC 1 cut(s) 205
BstSNI TACGTA 1 cut(s) 378
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuRI GGCC 1 cut(s) 327
BtsCI GGATG 3 cut(s) 88, 193, 232
BtsIMutI CAGTG 1 cut(s) 273
CfoI GCGC 3 cut(s) 175, 238, 361
Cfr13I GGNCC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 58
CviAII CATG 2 cut(s) 256, 362
CviJI RGCY 3 cut(s) 164, 304, 327
CviKI_1 RGCY 3 cut(s) 164, 304, 327
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 1 cut(s) 333
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
Eco105I TACGTA 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 197
Eco91I GGTNACC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 326
EcoO65I GGTNACC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 2 cut(s) 259, 365
FaiI YATR 7 cut(s) 30, 171, 257, 263, 265, 323, 363
FatI CATG 2 cut(s) 255, 361
FokI GGATG 3 cut(s) 95, 200, 239
GlaI GCGC 3 cut(s) 174, 237, 360
HaeIII GGCC 1 cut(s) 327
HhaI GCGC 3 cut(s) 175, 238, 361
Hin1II CATG 2 cut(s) 259, 365
Hin6I GCGC 3 cut(s) 173, 236, 359
HinP1I GCGC 3 cut(s) 173, 236, 359
HinfI GANTC 1 cut(s) 245
HphI GGTGA 2 cut(s) 217, 361
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 3 cut(s) 48, 135, 308
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 2 cut(s) 116, 377
HpyCH4V TGCA 2 cut(s) 64, 230
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 290
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 2 cut(s) 116, 377
Hsp92II CATG 2 cut(s) 259, 365
HspAI GCGC 3 cut(s) 173, 236, 359
Kzo9I GATC 1 cut(s) 142
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 3 cut(s) 159, 237, 261
LweI GCATC 2 cut(s) 131, 217
MaeII ACGT 2 cut(s) 116, 377
MaeIII GTNAC 1 cut(s) 205
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 1 cut(s) 190
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 2 cut(s) 9, 129
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 7 cut(s) 79, 133, 193, 196, 208, 279, 330
MseI TTAA 2 cut(s) 8, 300
MslI CAYNNNNRTG 2 cut(s) 276, 278
MwoI GCNNNNNNNGC 2 cut(s) 170, 290
NdeII GATC 1 cut(s) 142
NlaIII CATG 2 cut(s) 259, 365
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 1 cut(s) 259
OliI CACNNNNGTG 2 cut(s) 276, 278
PfeI GAWTC 1 cut(s) 245
Ppu21I YACGTR 1 cut(s) 378
PshBI ATTAAT 1 cut(s) 8
PspEI GGTNACC 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 326
PsrI GAACNNNNNNTAC 2 cut(s) 41, 73
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 2 cut(s) 276, 278
SaqAI TTAA 2 cut(s) 8, 300
Sau3AI GATC 1 cut(s) 142
Sau96I GGNCC 1 cut(s) 326
SduI GDGCHC 1 cut(s) 356
SetI ASST 5 cut(s) 119, 166, 207, 306, 380
SfaNI GCATC 2 cut(s) 131, 217
SmiMI CAYNNNNRTG 2 cut(s) 276, 278
SnaBI TACGTA 1 cut(s) 378
Sse9I AATT 2 cut(s) 9, 129
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 2 cut(s) 119, 380
TasI AATT 2 cut(s) 9, 129
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 8, 300
Tru9I TTAA 2 cut(s) 8, 300
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspRI CASTG 1 cut(s) 280
VspI ATTAAT 1 cut(s) 8
XceI RCATGY 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.