Rroxscaffold_3G00228730

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0018552
Physical Location & Seq
Forward (+)
13139261 .. 13158052
18792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228730.1

Sequence Viewer

Length: 483 bp
ATGATCCACCTGGTTAACGAAGCGGACAATCCTCGCAAACTCCATGGGGTCCCAAACCACGAAGCTTCTTCCAATAGAACCCCACGATATGATCAAATCCAACGACGGGTCGTCCACCAAATCAAACGTCTTTGGACGAAACGGCTGCACCGCAGTCTCCTGCGTACACCGAAGCGGCTGCTGCACTCCCACATAATCACTACTCCCGCCATTGAAAGAGCTGTGTATGCGCTAAAGAAGTTAGATGGGATTGTGGAGGAGGCGACCAAGGAGGAGTTGATGGATGCAATGGCGCAAGCGGATTCAACCGGCATGTTTATATGTCCTCACACCGGTGTGGCGTTGGCGGCGTTGATTAAGCTCGGATTTGGCCTGCCGTTTACCAAACACCTCAGCTGCTTCATCTCACAACAAGTTCGAAGTGCGTCATCTCACAGCAAGTTCGAAAGTGCTTCTTCTCACAAGACAACAATCCCAAACTAA

Protein Analysis

160

Amino Acids

18.03

Weight (kDa)

10.07

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018552)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0356311 RchiOBHm_Chr4g0415221
rosa_laevigata RLG00000009747
rosa_multiflora Rmu_co8170908.1_g000001 Rmu_co8374445.1_g000001
rosa_roxburghii Rroxscaffold_3G00228730 Rroxscaffold_5G00347460
rosa_rugosa Rorug03G0221500.1
rosa_samantha Rh2AG556000 Rh3DG267000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 23, 151, 175, 207, 299, 347
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 2 cut(s) 106, 332
AgeI ACCGGT 1 cut(s) 332
AgsI TTSAA 2 cut(s) 215, 306
AhdI GACNNNNNGTC 1 cut(s) 110
AjnI CCWGG 1 cut(s) 9
AleI CACNNNNGTG 2 cut(s) 333, 335
AluBI AGCT 4 cut(s) 65, 221, 361, 396
AluI AGCT 4 cut(s) 65, 221, 361, 396
Alw26I GTCTC 1 cut(s) 161
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 4 cut(s) 145, 178, 181, 396
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
AsiGI ACCGGT 1 cut(s) 332
AspLEI GCGC 2 cut(s) 232, 295
AspS9I GGNCC 1 cut(s) 49
AsuII TTCGAA 2 cut(s) 418, 444
AvaII GGWCC 1 cut(s) 49
BbvCI CCTCAGC 1 cut(s) 392
BbvI GCAGC 4 cut(s) 132, 165, 168, 383
BccI CCATC 2 cut(s) 239, 274
BceAI ACGGC 2 cut(s) 158, 361
BcgI CGANNNNNNTGC 4 cut(s) 127, 160, 161, 194
BciT130I CCWGG 1 cut(s) 11
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 161
BisI GCNGC 6 cut(s) 146, 176, 179, 182, 348, 397
BlsI GCNGC 6 cut(s) 147, 177, 180, 183, 349, 398
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 49
BmeRI GACNNNNNGTC 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 2 cut(s) 50, 51
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 1 cut(s) 274
Bpu10I CCTNAGC 1 cut(s) 392
Bpu14I TTCGAA 2 cut(s) 418, 444
BsaJI CCNNGG 2 cut(s) 43, 267
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 332
Bse118I RCCGGY 2 cut(s) 308, 332
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 2 cut(s) 43, 267
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 106, 332
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 406
BseRI GAGGAG 2 cut(s) 272, 287
BseXI GCAGC 4 cut(s) 132, 165, 168, 383
BsgI GTGCAG 2 cut(s) 131, 167
BshFI GGCC 1 cut(s) 372
BshTI ACCGGT 1 cut(s) 332
BsiSI CCGG 2 cut(s) 309, 333
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 106, 332
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 1 cut(s) 372
Bsp119I TTCGAA 2 cut(s) 418, 444
Bsp143I GATC 2 cut(s) 3, 91
Bsp19I CCATGG 1 cut(s) 43
BspACI CCGC 6 cut(s) 23, 151, 175, 207, 299, 347
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 1 cut(s) 405
BspLI GGNNCC 2 cut(s) 50, 51
BspT104I TTCGAA 2 cut(s) 418, 444
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 2 cut(s) 308, 332
BssAI RCCGGY 2 cut(s) 308, 332
BssECI CCNNGG 2 cut(s) 43, 267
BssMI GATC 2 cut(s) 3, 91
BssT1I CCWWGG 2 cut(s) 43, 267
Bst2UI CCWGG 1 cut(s) 11
BstBI TTCGAA 2 cut(s) 418, 444
BstC8I GCNNGC 2 cut(s) 297, 374
BstDEI CTNAG 1 cut(s) 392
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 1 cut(s) 289
BstHHI GCGC 2 cut(s) 232, 295
BstKTI GATC 2 cut(s) 6, 94
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 2 cut(s) 3, 91
BstMWI GCNNNNNNNGC 3 cut(s) 181, 227, 347
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 9
BstV1I GCAGC 4 cut(s) 132, 165, 168, 383
BsuRI GGCC 1 cut(s) 372
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 297, 374
CfoI GCGC 2 cut(s) 232, 295
Cfr10I RCCGGY 2 cut(s) 308, 332
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 1 cut(s) 414
CsiI ACCWGGT 1 cut(s) 9
Csp6I GTAC 1 cut(s) 165
CspAI ACCGGT 1 cut(s) 332
CviAII CATG 2 cut(s) 44, 313
CviJI RGCY 7 cut(s) 65, 145, 178, 221, 361, 372, 396
CviKI_1 RGCY 7 cut(s) 65, 145, 178, 221, 361, 372, 396
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 1 cut(s) 392
DpnI GATC 2 cut(s) 5, 93
DpnII GATC 2 cut(s) 3, 91
DriI GACNNNNNGTC 1 cut(s) 110
Eam1105I GACNNNNNGTC 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 43, 267
Eco47I GGWCC 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 2 cut(s) 43, 267
ErhI CCWWGG 2 cut(s) 43, 267
FaeI CATG 2 cut(s) 47, 316
FaiI YATR 7 cut(s) 45, 90, 194, 228, 314, 320, 322
FalI AAGNNNNNCTT 2 cut(s) 439, 471
FaqI GGGAC 1 cut(s) 35
FatI CATG 2 cut(s) 43, 312
FauI CCCGC 1 cut(s) 214
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 6 cut(s) 146, 176, 179, 182, 348, 397
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 6 cut(s) 146, 176, 179, 182, 348, 397
GlaI GCGC 2 cut(s) 231, 294
GluI GCNGC 6 cut(s) 146, 176, 179, 182, 348, 397
HaeIII GGCC 1 cut(s) 372
HapII CCGG 2 cut(s) 309, 333
HgaI GACGC 1 cut(s) 414
HhaI GCGC 2 cut(s) 232, 295
Hin1II CATG 2 cut(s) 47, 316
Hin6I GCGC 2 cut(s) 230, 293
HinP1I GCGC 2 cut(s) 230, 293
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 1 cut(s) 302
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 2 cut(s) 309, 333
Hpy166II GTNNAC 4 cut(s) 16, 115, 167, 381
Hpy188I TCNGA 1 cut(s) 365
Hpy8I GTNNAC 4 cut(s) 16, 115, 167, 381
Hpy99I CGWCG 1 cut(s) 108
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 3 cut(s) 148, 184, 287
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 227, 347
HpyF3I CTNAG 1 cut(s) 392
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 2 cut(s) 47, 316
HspAI GCGC 2 cut(s) 230, 293
KflI GGGWCCC 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 16
Kzo9I GATC 2 cut(s) 3, 91
LpnPI CCDG 5 cut(s) 23, 173, 322, 346, 386
Lsp1109I GCAGC 4 cut(s) 132, 165, 168, 383
LweI GCATC 1 cut(s) 274
MabI ACCWGGT 1 cut(s) 9
MaeII ACGT 1 cut(s) 127
MalI GATC 2 cut(s) 5, 93
MboI GATC 2 cut(s) 3, 91
MboII GAAGA 2 cut(s) 60, 447
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 6 cut(s) 42, 250, 253, 265, 336, 401
MseI TTAA 2 cut(s) 15, 357
MslI CAYNNNNRTG 2 cut(s) 333, 335
MspA1I CMGCKG 1 cut(s) 396
MspI CCGG 2 cut(s) 309, 333
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 3 cut(s) 181, 227, 347
NcoI CCATGG 1 cut(s) 43
NdeII GATC 2 cut(s) 3, 91
NlaIII CATG 2 cut(s) 47, 316
NlaIV GGNNCC 2 cut(s) 50, 51
NspI RCATGY 1 cut(s) 316
NspV TTCGAA 2 cut(s) 418, 444
OliI CACNNNNGTG 2 cut(s) 333, 335
PfeI GAWTC 1 cut(s) 302
PinAI ACCGGT 1 cut(s) 332
PkrI GCNGC 6 cut(s) 147, 177, 180, 183, 349, 398
PpuMI RGGWCCY 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 50, 51
PspPI GGNCC 1 cut(s) 49
PspPPI RGGWCCY 1 cut(s) 49
PvuII CAGCTG 1 cut(s) 396
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
RseI CAYNNNNRTG 2 cut(s) 333, 335
SaqAI TTAA 2 cut(s) 15, 357
SatI GCNGC 6 cut(s) 146, 176, 179, 182, 348, 397
Sau3AI GATC 2 cut(s) 3, 91
Sau96I GGNCC 1 cut(s) 49
ScrFI CCNGG 1 cut(s) 11
SetI ASST 7 cut(s) 12, 67, 130, 223, 363, 393, 398
SexAI ACCWGGT 1 cut(s) 9
SfaNI GCATC 1 cut(s) 274
SfuI TTCGAA 2 cut(s) 418, 444
SgrAI CRCCGGYG 1 cut(s) 332
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 2 cut(s) 333, 335
SsiI CCGC 6 cut(s) 23, 151, 175, 207, 299, 347
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 2 cut(s) 43, 267
TaiI ACGT 1 cut(s) 130
TaqI TCGA 2 cut(s) 418, 444
TauI GCSGC 2 cut(s) 178, 350
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 2 cut(s) 15, 357
Tru9I TTAA 2 cut(s) 15, 357
TseI GCWGC 4 cut(s) 145, 178, 181, 396
TspDTI ATGAA 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 49
XceI RCATGY 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.