Rmu_co8170908.1_g000001

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8170908.1
OG0018552
Physical Location & Seq
Forward (+)
1 .. 645
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8170908.1_g000001.1.cds

Sequence Viewer

Length: 645 bp
gctattgagattctgcagcagtttgattggcaggtcccggattgggttatagttccgggaggcaatctcggaaatatatatgcattctacaaagggtttcatatgtgtaaggagttggggctggttgataagattcctagacttgtgtgtgctcaagctgcaaatgcaaaccctttgtacttgcattacaagtcggggtgggatgaggagttcaagcctgtgaaggcaaacactacgtttgcgtctgcaattcagattggggatcctgtttccattgacagggccgtgtatgcgctgaagaagtcggatgggattgtggaggaggcgaccgaggaggagttgatggatgcaatggcgcaagcggattctaccggcatgtttatatgtcctcacactggtgtggcgctggcggcgttgatcaagctccggaataagggggttataggccccactgagaggactgtggtggtgagcacggctcatgggttgaagttcacgcaggcgaagattgattatcactccaaggagattccggatttggcttgccggtttgctaacccaccggttcaagtgagggcagattttgggtcggttatggatgttttgaagaagtacttgagtaagtcggaggctccaaagcattag

Protein Analysis

214

Amino Acids

23.55

Weight (kDa)

6.33

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018552)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0356311 RchiOBHm_Chr4g0415221
rosa_laevigata RLG00000009747
rosa_multiflora Rmu_co8170908.1_g000001 Rmu_co8374445.1_g000001
rosa_roxburghii Rroxscaffold_3G00228730 Rroxscaffold_5G00347460
rosa_rugosa Rorug03G0221500.1
rosa_samantha Rh2AG556000 Rh3DG267000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 22
AccIII TCCGGA 2 cut(s) 426, 532
AciI CCGC 2 cut(s) 362, 410
AclWI GGATC 2 cut(s) 257, 270
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 2 cut(s) 179, 614
AfiI CCNNNNNNNGG 5 cut(s) 43, 279, 395, 433, 456
AgeI ACCGGT 1 cut(s) 562
AgsI TTSAA 4 cut(s) 214, 490, 569, 607
AleI CACNNNNGTG 2 cut(s) 396, 398
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 2 cut(s) 158, 424
AluI AGCT 2 cut(s) 158, 424
Alw21I GWGCWC 2 cut(s) 154, 476
AlwI GGATC 2 cut(s) 257, 270
Aor13HI TCCGGA 2 cut(s) 426, 532
AoxI GGCC 2 cut(s) 282, 445
ApeKI GCWGC 2 cut(s) 16, 158
AsiGI ACCGGT 1 cut(s) 562
AspLEI GCGC 3 cut(s) 295, 358, 406
AspS9I GGNCC 3 cut(s) 34, 282, 446
AsuC2I CCSGG 2 cut(s) 38, 57
AsuHPI GGTGA 1 cut(s) 481
AvaII GGWCC 1 cut(s) 34
BamHI GGATCC 1 cut(s) 262
Bbv12I GWGCWC 2 cut(s) 154, 476
BbvI GCAGC 2 cut(s) 28, 145
BccI CCATC 2 cut(s) 302, 337
BceAI ACGGC 2 cut(s) 269, 492
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 2 cut(s) 38, 57
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 3 cut(s) 17, 159, 411
BlsI GCNGC 3 cut(s) 18, 160, 412
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 2 cut(s) 38, 57
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 3 cut(s) 34, 282, 446
BmiI GGNNCC 4 cut(s) 36, 264, 448, 633
BmrFI CCNGG 2 cut(s) 38, 57
BmsI GCATC 1 cut(s) 337
BplI GAGNNNNNCTC 4 cut(s) 51, 83, 463, 495
BpuEI CTTGAG 2 cut(s) 138, 637
BpuMI CCSGG 2 cut(s) 38, 57
BsaJI CCNNGG 2 cut(s) 330, 522
BsaWI WCCGGW 3 cut(s) 426, 532, 562
Bsc4I CCNNNNNNNGG 5 cut(s) 43, 279, 395, 433, 456
Bse118I RCCGGY 3 cut(s) 371, 546, 562
Bse1I ACTGG 1 cut(s) 400
Bse3DI GCAATG 1 cut(s) 357
BseAI TCCGGA 2 cut(s) 426, 532
BseDI CCNNGG 2 cut(s) 330, 522
BseGI GGATG 4 cut(s) 208, 313, 352, 604
BseLI CCNNNNNNNGG 5 cut(s) 43, 279, 395, 433, 456
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 4 cut(s) 221, 335, 347, 350
BseXI GCAGC 2 cut(s) 28, 145
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 2 cut(s) 284, 447
BshTI ACCGGT 1 cut(s) 562
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 2 cut(s) 154, 476
BsiSI CCGG 7 cut(s) 38, 56, 372, 427, 533, 547, 563
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 5 cut(s) 43, 279, 395, 433, 456
BsmFI GGGAC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 2 cut(s) 284, 447
Bsp1286I GDGCHC 2 cut(s) 154, 476
Bsp13I TCCGGA 2 cut(s) 426, 532
Bsp143I GATC 2 cut(s) 262, 417
BspACI CCGC 2 cut(s) 362, 410
BspANI GGCC 2 cut(s) 284, 447
BspCNI CTCAG 1 cut(s) 445
BspEI TCCGGA 2 cut(s) 426, 532
BspLI GGNNCC 4 cut(s) 36, 264, 448, 633
BspMAI CTGCAG 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 2 cut(s) 257, 270
BsrDI GCAATG 1 cut(s) 357
BsrFI RCCGGY 3 cut(s) 371, 546, 562
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 3 cut(s) 371, 546, 562
BssECI CCNNGG 2 cut(s) 330, 522
BssMI GATC 2 cut(s) 262, 417
BssT1I CCWWGG 1 cut(s) 522
Bst4CI ACNGT 1 cut(s) 463
BstC8I GCNNGC 4 cut(s) 360, 408, 501, 544
BstDEI CTNAG 1 cut(s) 453
BstF5I GGATG 4 cut(s) 208, 313, 352, 604
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 3 cut(s) 295, 358, 406
BstKTI GATC 2 cut(s) 265, 420
BstMBI GATC 2 cut(s) 262, 417
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 4 cut(s) 158, 164, 290, 410
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 36, 55
BstSFI CTRYAG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 28, 145
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BsuRI GGCC 2 cut(s) 284, 447
BtsCI GGATG 4 cut(s) 208, 313, 352, 604
BtsIMutI CAGTG 2 cut(s) 393, 450
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 4 cut(s) 360, 408, 501, 544
CfoI GCGC 3 cut(s) 295, 358, 406
Cfr10I RCCGGY 3 cut(s) 371, 546, 562
Cfr13I GGNCC 3 cut(s) 34, 282, 446
CseI GACGC 1 cut(s) 231
Csp6I GTAC 2 cut(s) 178, 613
CspAI ACCGGT 1 cut(s) 562
CviAII CATG 2 cut(s) 376, 482
CviJI RGCY 9 cut(s) 121, 158, 217, 284, 424, 447, 479, 542, 632
CviKI_1 RGCY 9 cut(s) 121, 158, 217, 284, 424, 447, 479, 542, 632
CviQI GTAC 2 cut(s) 178, 613
DdeI CTNAG 1 cut(s) 453
DpnI GATC 2 cut(s) 264, 419
DpnII GATC 2 cut(s) 262, 417
Eco130I CCWWGG 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 317
EcoO109I RGGNCCY 2 cut(s) 34, 446
EcoT14I CCWWGG 1 cut(s) 522
EcoT22I ATGCAT 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 2 cut(s) 379, 485
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FaqI GGGAC 1 cut(s) 20
FatI CATG 2 cut(s) 375, 481
FauNDI CATATG 1 cut(s) 102
FbaI TGATCA 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 17, 159, 411
FokI GGATG 4 cut(s) 215, 320, 359, 611
Fsp4HI GCNGC 3 cut(s) 17, 159, 411
FspBI CTAG 1 cut(s) 138
GlaI GCGC 3 cut(s) 294, 357, 405
GluI GCNGC 3 cut(s) 17, 159, 411
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 2 cut(s) 284, 447
HapII CCGG 7 cut(s) 38, 56, 372, 427, 533, 547, 563
HgaI GACGC 1 cut(s) 231
HhaI GCGC 3 cut(s) 295, 358, 406
Hin1II CATG 2 cut(s) 379, 485
Hin6I GCGC 3 cut(s) 293, 356, 404
HinP1I GCGC 3 cut(s) 293, 356, 404
HinfI GANTC 4 cut(s) 10, 133, 365, 529
HpaII CCGG 7 cut(s) 38, 56, 372, 427, 533, 547, 563
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 4 cut(s) 71, 255, 307, 628
Hpy188III TCNNGA 2 cut(s) 427, 533
Hpy8I GTNNAC 1 cut(s) 495
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 7 cut(s) 16, 83, 161, 167, 184, 248, 350
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 164, 290, 410
HpyF3I CTNAG 1 cut(s) 453
HpySE526I ACGT 1 cut(s) 236
Hsp92II CATG 2 cut(s) 379, 485
HspAI GCGC 3 cut(s) 293, 356, 404
Kpn2I TCCGGA 2 cut(s) 426, 532
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 2 cut(s) 262, 417
LmnI GCTCC 2 cut(s) 429, 637
Lsp1109I GCAGC 2 cut(s) 28, 145
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 236
MalI GATC 2 cut(s) 264, 419
MboI GATC 2 cut(s) 262, 417
MboII GAAGA 3 cut(s) 310, 517, 619
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 154, 476
MluCI AATT 1 cut(s) 249
MmeI TCCRAC 2 cut(s) 285, 606
Mph1103I ATGCAT 1 cut(s) 85
MroI TCCGGA 2 cut(s) 426, 532
MslI CAYNNNNRTG 2 cut(s) 396, 398
MspI CCGG 7 cut(s) 38, 56, 372, 427, 533, 547, 563
MspR9I CCNGG 2 cut(s) 38, 57
Mva1269I GAATGC 1 cut(s) 83
MwoI GCNNNNNNNGC 4 cut(s) 158, 164, 290, 410
NciI CCSGG 2 cut(s) 38, 57
NdeI CATATG 1 cut(s) 102
NdeII GATC 2 cut(s) 262, 417
NlaIII CATG 2 cut(s) 379, 485
NlaIV GGNNCC 4 cut(s) 36, 264, 448, 633
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 1 cut(s) 379
OliI CACNNNNGTG 2 cut(s) 396, 398
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 4 cut(s) 10, 133, 365, 529
PfoI TCCNGGA 2 cut(s) 36, 55
PinAI ACCGGT 1 cut(s) 562
PkrI GCNGC 3 cut(s) 18, 160, 412
PpuMI RGGWCCY 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 34
PspN4I GGNNCC 4 cut(s) 36, 264, 448, 633
PspPI GGNCC 3 cut(s) 34, 282, 446
PspPPI RGGWCCY 1 cut(s) 34
PstI CTGCAG 1 cut(s) 18
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 2 cut(s) 179, 614
RsaNI GTAC 2 cut(s) 178, 613
RseI CAYNNNNRTG 2 cut(s) 396, 398
SatI GCNGC 3 cut(s) 17, 159, 411
Sau3AI GATC 2 cut(s) 262, 417
Sau96I GGNCC 3 cut(s) 34, 282, 446
ScaI AGTACT 1 cut(s) 614
ScrFI CCNGG 2 cut(s) 38, 57
SduI GDGCHC 2 cut(s) 154, 476
SetI ASST 4 cut(s) 36, 160, 239, 426
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 1 cut(s) 14
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 2 cut(s) 396, 398
SmlI CTYRAG 2 cut(s) 153, 616
SmoI CTYRAG 2 cut(s) 153, 616
Sse9I AATT 1 cut(s) 249
SsiI CCGC 2 cut(s) 362, 410
SspMI CTAG 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 36, 55
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 463
TaiI ACGT 1 cut(s) 239
TaqII GACCGA 1 cut(s) 344
TasI AATT 1 cut(s) 249
TatI WGTACW 2 cut(s) 177, 612
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 4 cut(s) 10, 133, 365, 529
TscAI CASTG 2 cut(s) 400, 457
TseI GCWGC 2 cut(s) 16, 158
TspDTI ATGAA 1 cut(s) 89
TspRI CASTG 2 cut(s) 400, 457
VpaK11BI GGWCC 1 cut(s) 34
XceI RCATGY 1 cut(s) 379
XspI CTAG 1 cut(s) 138
ZrmI AGTACT 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.