RLG00000009747

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018552
Physical Location & Seq
Forward (+)
57296547 .. 57298034
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009747

Sequence Viewer

Length: 549 bp
ATGATCAAATCCAGCGACGGGTCGTCCACCAAATCAAACGTCTTTGGACAGAAACGGTGGCACCGGAGTCTCCTGCAGACACTGAAGCTGTTGTGGCACTCCCACATAATCACTACTCCCACCACTGAAAGGTTCAACCTTGCTCTCCTCACCATGCTCTCAGTTCTGCAGTCACTCCAATTATATGTAGAGTTTCGCAGAGAGTTGAACTCGCCCAGCACATTTACAATGGATTTGGAAGTTTTGGGGCATGAGTTGCAGTTCATTCAGGATCCTAATTCAAAGCATCTAGGAACTATGGTTTGGGATGCATCACTGGTATTCGCCAAGTTTCTGATTAGGGATCCTGTTTGGATTGACAGAGCTGTGTATGCGCTGAAGAAGTCGGATGGGATTGTGGAGGAGGTGACCGAGGAGGAGTTGATGGATGCAATGGCGCAAGCAGATTCTACTGGCATGTTTATATGTCCACACACTGTTGTGGCACTGGAGGCGTTGATTAAGCTCCGGATTTGGCCTGCCAGTTTGCTAACCCACCGGTTCAGGTGA

Protein Analysis

183

Amino Acids

20.89

Weight (kDa)

6.65

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018552)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0356311 RchiOBHm_Chr4g0415221
rosa_laevigata RLG00000009747
rosa_multiflora Rmu_co8170908.1_g000001 Rmu_co8374445.1_g000001
rosa_roxburghii Rroxscaffold_3G00228730 Rroxscaffold_5G00347460
rosa_rugosa Rorug03G0221500.1
rosa_samantha Rh2AG556000 Rh3DG267000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 507
AclWI GGATC 4 cut(s) 266, 279, 338, 351
AcuI CTGAAG 2 cut(s) 104, 398
AfiI CCNNNNNNNGG 2 cut(s) 18, 129
AgeI ACCGGT 1 cut(s) 537
AgsI TTSAA 3 cut(s) 136, 208, 282
AhdI GACNNNNNGTC 1 cut(s) 22
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
AleI CACNNNNGTG 1 cut(s) 479
AluBI AGCT 3 cut(s) 88, 365, 505
AluI AGCT 3 cut(s) 88, 365, 505
Alw26I GTCTC 1 cut(s) 74
AlwI GGATC 4 cut(s) 266, 279, 338, 351
AlwNI CAGNNNCTG 1 cut(s) 82
Aor13HI TCCGGA 1 cut(s) 507
AoxI GGCC 1 cut(s) 515
ArsI GACNNNNNNTTYG 2 cut(s) 24, 56
AsiGI ACCGGT 1 cut(s) 537
AspLEI GCGC 2 cut(s) 376, 439
AsuHPI GGTGA 2 cut(s) 142, 418
BamHI GGATCC 2 cut(s) 271, 343
BanI GGYRCC 1 cut(s) 60
BccI CCATC 2 cut(s) 383, 418
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 2 cut(s) 74, 167
BmeRI GACNNNNNGTC 1 cut(s) 22
BmiI GGNNCC 3 cut(s) 62, 273, 345
BmsI GCATC 4 cut(s) 295, 298, 320, 418
BplI GAGNNNNNCTC 2 cut(s) 194, 226
BpmI CTGGAG 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 3 cut(s) 63, 507, 537
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 129
Bse118I RCCGGY 1 cut(s) 537
Bse1I ACTGG 4 cut(s) 321, 457, 492, 522
Bse3DI GCAATG 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 507
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 3 cut(s) 313, 394, 433
BseLI CCNNNNNNNGG 2 cut(s) 18, 129
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 4 cut(s) 321, 457, 492, 522
BseRI GAGGAG 4 cut(s) 137, 416, 428, 431
BseYI CCCAGC 1 cut(s) 215
BshFI GGCC 1 cut(s) 517
BshNI GGYRCC 1 cut(s) 60
BshTI ACCGGT 1 cut(s) 537
BsiSI CCGG 3 cut(s) 64, 508, 538
BslI CCNNNNNNNGG 2 cut(s) 18, 129
BsmAI GTCTC 1 cut(s) 74
BsnI GGCC 1 cut(s) 517
Bsp13I TCCGGA 1 cut(s) 507
Bsp143I GATC 3 cut(s) 3, 271, 343
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 1 cut(s) 173
BspEI TCCGGA 1 cut(s) 507
BspLI GGNNCC 3 cut(s) 62, 273, 345
BspMAI CTGCAG 2 cut(s) 78, 171
BspPI GGATC 4 cut(s) 266, 279, 338, 351
BspT107I GGYRCC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 537
BsrI ACTGG 4 cut(s) 321, 457, 492, 522
BssAI RCCGGY 1 cut(s) 537
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 3 cut(s) 3, 271, 343
Bst4CI ACNGT 2 cut(s) 57, 478
BstAPI GCANNNNNTGC 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 441, 519
BstDEI CTNAG 1 cut(s) 160
BstEII GGTNACC 1 cut(s) 406
BstF5I GGATG 3 cut(s) 313, 394, 433
BstHHI GCGC 2 cut(s) 376, 439
BstKTI GATC 3 cut(s) 6, 274, 346
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 3 cut(s) 3, 271, 343
BstMWI GCNNNNNNNGC 4 cut(s) 94, 256, 371, 491
BstNSI RCATGY 1 cut(s) 460
BstPI GGTNACC 1 cut(s) 406
BstSFI CTRYAG 2 cut(s) 74, 167
BstX2I RGATCY 2 cut(s) 271, 343
BstYI RGATCY 2 cut(s) 271, 343
BsuRI GGCC 1 cut(s) 517
BtsCI GGATG 3 cut(s) 313, 394, 433
BtsIMutI CAGTG 5 cut(s) 80, 123, 314, 474, 485
Cac8I GCNNGC 2 cut(s) 441, 519
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 2 cut(s) 376, 439
Cfr10I RCCGGY 1 cut(s) 537
CspAI ACCGGT 1 cut(s) 537
CviAII CATG 3 cut(s) 154, 251, 457
CviJI RGCY 4 cut(s) 88, 365, 505, 517
CviKI_1 RGCY 4 cut(s) 88, 365, 505, 517
DdeI CTNAG 1 cut(s) 160
DpnI GATC 3 cut(s) 5, 273, 345
DpnII GATC 3 cut(s) 3, 271, 343
DriI GACNNNNNGTC 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 104, 398
Eco91I GGTNACC 1 cut(s) 406
EcoO65I GGTNACC 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 313
FaeI CATG 3 cut(s) 157, 254, 460
FatI CATG 3 cut(s) 153, 250, 456
FbaI TGATCA 1 cut(s) 3
FokI GGATG 3 cut(s) 320, 401, 440
FspBI CTAG 1 cut(s) 290
GlaI GCGC 2 cut(s) 375, 438
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 509
HaeIII GGCC 1 cut(s) 517
HapII CCGG 3 cut(s) 64, 508, 538
HhaI GCGC 2 cut(s) 376, 439
Hin1II CATG 3 cut(s) 157, 254, 460
Hin6I GCGC 2 cut(s) 374, 437
HinP1I GCGC 2 cut(s) 374, 437
HinfI GANTC 2 cut(s) 67, 446
HpaII CCGG 3 cut(s) 64, 508, 538
HphI GGTGA 2 cut(s) 142, 418
Hpy166II GTNNAC 2 cut(s) 27, 470
Hpy188I TCNGA 2 cut(s) 336, 388
Hpy188III TCNNGA 2 cut(s) 269, 508
Hpy8I GTNNAC 2 cut(s) 27, 470
Hpy99I CGWCG 1 cut(s) 20
HpyCH4III ACNGT 2 cut(s) 57, 478
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 5 cut(s) 76, 169, 259, 311, 431
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 256, 371, 491
HpyF3I CTNAG 1 cut(s) 160
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 3 cut(s) 157, 254, 460
HspAI GCGC 2 cut(s) 374, 437
Kpn2I TCCGGA 1 cut(s) 507
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 271, 343
LmnI GCTCC 1 cut(s) 510
LweI GCATC 4 cut(s) 295, 298, 320, 418
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 2 cut(s) 171, 406
MalI GATC 3 cut(s) 5, 273, 345
MboI GATC 3 cut(s) 3, 271, 343
MboII GAAGA 1 cut(s) 391
MflI RGATCY 2 cut(s) 271, 343
MluCI AATT 2 cut(s) 179, 277
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 6 cut(s) 158, 394, 397, 406, 409, 484
Mph1103I ATGCAT 1 cut(s) 313
MroI TCCGGA 1 cut(s) 507
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 1 cut(s) 479
MspI CCGG 3 cut(s) 64, 508, 538
MwoI GCNNNNNNNGC 4 cut(s) 94, 256, 371, 491
NdeII GATC 3 cut(s) 3, 271, 343
NlaIII CATG 3 cut(s) 157, 254, 460
NlaIV GGNNCC 3 cut(s) 62, 273, 345
NmuCI GTSAC 2 cut(s) 171, 406
NsiI ATGCAT 1 cut(s) 313
NspI RCATGY 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 479
PfeI GAWTC 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 537
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PspEI GGTNACC 1 cut(s) 406
PspFI CCCAGC 1 cut(s) 215
PspN4I GGNNCC 3 cut(s) 62, 273, 345
PstI CTGCAG 2 cut(s) 78, 171
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 271, 343
RseI CAYNNNNRTG 1 cut(s) 479
SaqAI TTAA 1 cut(s) 501
Sau3AI GATC 3 cut(s) 3, 271, 343
SchI GAGTC 1 cut(s) 76
SetI ASST 8 cut(s) 42, 90, 134, 141, 367, 408, 507, 548
SfaNI GCATC 4 cut(s) 295, 298, 320, 418
SfcI CTRYAG 2 cut(s) 74, 167
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 2 cut(s) 179, 277
SspMI CTAG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 57, 478
TaiI ACGT 1 cut(s) 42
TaqII GACCGA 1 cut(s) 425
TasI AATT 2 cut(s) 179, 277
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 5 cut(s) 87, 130, 321, 481, 492
TseFI GTSAC 2 cut(s) 171, 406
Tsp45I GTSAC 2 cut(s) 171, 406
TspDTI ATGAA 1 cut(s) 253
TspRI CASTG 5 cut(s) 87, 130, 321, 481, 492
XceI RCATGY 1 cut(s) 460
XspI CTAG 1 cut(s) 290
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.