RchiOBHm_Chr1g0357781

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016919
Physical Location & Seq
Forward (+)
50066588 .. 50067698
1111 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGTCTCTGTTTTTATCTTCAACAGCGAACATTTTAAGCTCTCTGTATTTGAAAGTGTCGGAGTTATTGGGCACTGCCAAGAACTATATTGTGAACAAGGCCAGGGGTTATGTTTTGGCAAGGTTGGTTGAGATGCAGAGGAGGTCTGCGCTTATTGTAGACCTTCAACTTGAGGATATAAGCTTCGAGTCCATAGATCTCCTTTTCAAGATCTCTGTGTACAACTCATTTCAGTTTTCTCTGCCTGTCAGTCAAGCCTCCTTCAAAATGAAAATCGGTGGCAGTGAACCTTTATTAGGGAAATTGAAGGAACCTGTGTACATAAAGGCAGGAGCAGATTCTATATTGCTTTTGTCAGCGAAGGTGCCATTTAGTATGCTTATAAATTTGGCAAATGACATTTCTGTAGATGCAGACGTTGACTACCGGATCGATTTCCAACTCACAATTCACCTTCCTATCATCGGCAACATCTCTTTACCACTTCCTTGCCAACAAGGCGAGACCAAGATCTCCGATTTGTTTAGCAATTTGATGGATCAAATCATGATGAGTTGGTGA

Protein Analysis

186

Amino Acids

20.7

Weight (kDa)

5.39

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 70 - 165 1.2e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016919)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g15470
malus_domestica MD01G1072000.v1.1
prunus_persica Prupe.2G178300_v2.0.a1
pyrus_communis pycom01g10120
rosa_chinensis RchiOBHm_Chr1g0357781
rosa_laevigata RLG00000027997
rosa_multiflora Rmu_sc0011109.1_g000014
rosa_roxburghii Rroxscaffold_4G00298520
rosa_rugosa Rorug01G0258600
rosa_samantha Rh1AG272500 Rh1BG239700 Rh1CG256400 Rh1DG268000
rosa_wichuraiana Rw1G024320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 159
AclWI GGATC 2 cut(s) 437, 546
AcsI RAATTY 1 cut(s) 385
AfaI GTAC 2 cut(s) 221, 320
AfiI CCNNNNNNNGG 2 cut(s) 296, 464
AgsI TTSAA 6 cut(s) 21, 52, 167, 208, 265, 307
AjnI CCWGG 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 2 cut(s) 39, 183
AluI AGCT 2 cut(s) 39, 183
Alw26I GTCTC 2 cut(s) 9, 497
AlwI GGATC 2 cut(s) 437, 546
AoxI GGCC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 74
BanI GGYRCC 1 cut(s) 364
BccI CCATC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 9, 497
BfmI CTRYAG 1 cut(s) 405
BglI GCCNNNNNGGC 1 cut(s) 498
BglII AGATCT 3 cut(s) 196, 210, 510
Bme1390I CCNGG 1 cut(s) 103
BmiI GGNNCC 2 cut(s) 312, 366
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 2 cut(s) 123, 400
BpuEI CTTGAG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 1 cut(s) 426
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 464
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 296, 464
BseRI GAGGAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 74
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 432
BsiSI CCGG 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 296, 464
BsmAI GTCTC 2 cut(s) 9, 497
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 74
Bsp1407I TGTACA 2 cut(s) 219, 318
Bsp143I GATC 5 cut(s) 196, 210, 429, 510, 538
BspANI GGCC 1 cut(s) 101
BspDI ATCGAT 1 cut(s) 432
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 312, 366
BspPI GGATC 2 cut(s) 437, 546
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 497
BsrGI TGTACA 2 cut(s) 219, 318
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 5 cut(s) 196, 210, 429, 510, 538
Bst2UI CCWGG 1 cut(s) 103
BstAUI TGTACA 2 cut(s) 219, 318
BstENI CCTNNNNNAGG 1 cut(s) 294
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 5 cut(s) 199, 213, 432, 513, 541
BstMAI GTCTC 2 cut(s) 9, 497
BstMBI GATC 5 cut(s) 196, 210, 429, 510, 538
BstMWI GCNNNNNNNGC 1 cut(s) 498
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 74
BstX2I RGATCY 3 cut(s) 196, 210, 510
BstYI RGATCY 3 cut(s) 196, 210, 510
Bsu15I ATCGAT 1 cut(s) 432
BsuRI GGCC 1 cut(s) 101
BsuTUI ATCGAT 1 cut(s) 432
BtsI GCAGTG 2 cut(s) 72, 289
BtsIMutI CAGTG 2 cut(s) 72, 289
CciI TCATGA 1 cut(s) 546
CfoI GCGC 1 cut(s) 151
ClaI ATCGAT 1 cut(s) 432
Csp6I GTAC 2 cut(s) 220, 319
CviAII CATG 1 cut(s) 547
CviJI RGCY 4 cut(s) 39, 101, 183, 257
CviKI_1 RGCY 4 cut(s) 39, 101, 183, 257
CviQI GTAC 2 cut(s) 220, 319
DpnI GATC 5 cut(s) 198, 212, 431, 512, 540
DpnII GATC 5 cut(s) 196, 210, 429, 510, 538
Eco31I GGTCTC 1 cut(s) 497
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 101
FaeI CATG 1 cut(s) 550
FaiI YATR 9 cut(s) 87, 111, 179, 194, 323, 344, 377, 383, 548
FatI CATG 1 cut(s) 546
FblI GTMKAC 1 cut(s) 159
GlaI GCGC 1 cut(s) 150
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 427
HhaI GCGC 1 cut(s) 151
Hin1II CATG 1 cut(s) 550
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 2 cut(s) 188, 338
HpaII CCGG 1 cut(s) 427
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 6 cut(s) 94, 160, 220, 287, 319, 421
Hpy188I TCNGA 2 cut(s) 61, 517
Hpy188III TCNNGA 2 cut(s) 208, 547
Hpy8I GTNNAC 6 cut(s) 94, 160, 220, 287, 319, 421
HpyAV CCTTC 5 cut(s) 173, 271, 301, 355, 464
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 2 cut(s) 136, 413
HpyF10VI GCNNNNNNNGC 1 cut(s) 498
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 1 cut(s) 550
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 5 cut(s) 196, 210, 429, 510, 538
LmnI GCTCC 1 cut(s) 332
LpnPI CCDG 6 cut(s) 88, 115, 258, 315, 327, 440
LweI GCATC 2 cut(s) 123, 400
MaeII ACGT 1 cut(s) 417
MalI GATC 5 cut(s) 198, 212, 431, 512, 540
MboI GATC 5 cut(s) 196, 210, 429, 510, 538
MboII GAAGA 1 cut(s) 9
MflI RGATCY 3 cut(s) 196, 210, 510
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 4 cut(s) 302, 385, 447, 529
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 39, 463
MnlI CCTC 4 cut(s) 132, 135, 166, 268
MseI TTAA 1 cut(s) 35
MspI CCGG 1 cut(s) 427
MspR9I CCNGG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 1 cut(s) 498
NdeII GATC 5 cut(s) 196, 210, 429, 510, 538
NlaIII CATG 1 cut(s) 550
NlaIV GGNNCC 2 cut(s) 312, 366
PagI TCATGA 1 cut(s) 546
PfeI GAWTC 1 cut(s) 338
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PsiI TTATAA 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 312, 366
PsuI RGATCY 3 cut(s) 196, 210, 510
RsaI GTAC 2 cut(s) 221, 320
RsaNI GTAC 2 cut(s) 220, 319
SaqAI TTAA 1 cut(s) 35
Sau3AI GATC 5 cut(s) 196, 210, 429, 510, 538
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 1 cut(s) 74
SfaNI GCATC 2 cut(s) 123, 400
SfcI CTRYAG 1 cut(s) 405
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 4 cut(s) 302, 385, 447, 529
StyD4I CCNGG 1 cut(s) 101
TaiI ACGT 1 cut(s) 420
TaqI TCGA 2 cut(s) 186, 432
TasI AATT 4 cut(s) 302, 385, 447, 529
TatI WGTACW 2 cut(s) 219, 318
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 2 cut(s) 79, 289
TspDTI ATGAA 1 cut(s) 284
TspRI CASTG 2 cut(s) 79, 289
XagI CCTNNNNNAGG 1 cut(s) 294
XapI RAATTY 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.