RchiOBHm_Chr1g0358591

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009202
Physical Location & Seq
Reverse (-)
50707844 .. 50711316
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1170 bp
ATGGGAGGATCTTCAACAAAAGCTCTTGTTCACCAGAAGCTTTTACTTGTATGGACGATAGTGGTGGCAACGTTGAGCTCTTTCAGTGATTCTAAATGTGATTTCGAGGCGATCTTCAACTTCGGTGACTCGAATTCAGATACCGGCGGGTTTTGGGCAGCATTTCCCGCACAGTCTGGACCGTTCGGCATGACCTATTTCAAGAAACCGACAGGTCGGGCTTCCGATGGGAGGCTCATTGTTGATTTCTTGGCTCAAGCTCTAGGATTGCCATTCCTGAGTCCATATCTACAATCGATTGGATCCGATTATAGACATGGAGTAAACTTTGCGACATTGGCATCAACCGTGCTTTTGCCAAATACTTCCTTGTTTGTTACCGGAATCAGTCCATTCTCTCTAGCTATCCAGCTCAACCAAATGAAGGCATTCAAGACCCAAGTTGAAGAGTTTCATCACTCCAACTTGGATAAACAAGAACCCAAGAAACTTCCTTCCCCAGATATTTTTGGGAAATCACTCTACATGTTCTATATTGGTCAAAACGATTTTACTTCGAATTTAGCAGCCATTGGTGTAGGAGGAGTGAGGCAATATCTTCCTCAGGTGGTCTCCCAAATTGCTGGTACGGTCAGGGAGCTATATGCTTTAGGAGGGCGTGCATTTCTGGTACTTAATCTAGCACCAGTTGGTTGCTATCCCTCGTTTCTGGTAGGGCTTCCCCGTTCTGAACTCGATGCTTTTGGATGCTCGATTTCTTACAACAATGCAGTACAAGACTACAACAACATGCTTAAGGAGACGCTTGCACAAACAAGAGGCTCTCTCCCAAATGCTTCTCTAATATATGTGGACTCCTCATCTGCTTTGCTAGAGCTCTTTAGACACCCCACAATCCATGGGCTCAAATATGGTACCAAATCATGTTGTGGGCATGGAGGTGGTGCCTACAATTATGACCGTCAGGTTTATTGTGGAAATACAAAGGTGATCAATGGGAGCACTGTGACTGCAACAGCTTGTGATGATCCATACAATTATGTAAGCTGGGATGGAATCCATGCCACTGAAGCTGCAAACAAGCTTGTTACCTGGGCTATTCTCAACGGCTCTTATTTCGACCCACCTTTTCCTCTTCACCAACTTTGTGACCTCTACCCTATAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

42.24

Weight (kDa)

6.49

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 363 5.1e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 914
AccB1I GGYRCC 2 cut(s) 914, 944
AciI CCGC 2 cut(s) 147, 168
AclI AACGTT 1 cut(s) 71
AclWI GGATC 4 cut(s) 16, 297, 310, 1022
AcsI RAATTY 2 cut(s) 133, 559
AcuI CTGAAG 1 cut(s) 1089
AfaI GTAC 4 cut(s) 628, 672, 774, 916
AfiI CCNNNNNNNGG 2 cut(s) 231, 424
AflII CTTAAG 1 cut(s) 794
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 5 cut(s) 15, 118, 202, 433, 446
AjnI CCWGG 1 cut(s) 1091
Alw21I GWGCWC 3 cut(s) 80, 879, 1004
Alw26I GTCTC 2 cut(s) 616, 794
AlwI GGATC 4 cut(s) 16, 297, 310, 1022
ApeKI GCWGC 3 cut(s) 158, 566, 1073
ApoI RAATTY 2 cut(s) 133, 559
Asp700I GAANNNNTTC 2 cut(s) 428, 450
Asp718I GGTACC 1 cut(s) 914
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 4 cut(s) 23, 137, 1000, 1130
AsuII TTCGAA 1 cut(s) 557
AvaII GGWCC 1 cut(s) 179
AxyI CCTNAGG 1 cut(s) 603
BamHI GGATCC 1 cut(s) 302
BanI GGYRCC 2 cut(s) 914, 944
BanII GRGCYC 3 cut(s) 80, 879, 906
Bbv12I GWGCWC 3 cut(s) 80, 879, 1004
BbvI GCAGC 3 cut(s) 170, 578, 1060
BccI CCATC 2 cut(s) 221, 1046
BceAI ACGGC 1 cut(s) 1123
BciT130I CCWGG 1 cut(s) 1093
BclI TGATCA 1 cut(s) 990
BcoDI GTCTC 2 cut(s) 616, 794
BfaI CTAG 4 cut(s) 263, 401, 680, 872
BfmI CTRYAG 1 cut(s) 1161
BfrI CTTAAG 1 cut(s) 794
BisI GCNGC 3 cut(s) 159, 567, 1074
BlsI GCNGC 3 cut(s) 160, 568, 1075
Bme1390I CCNGG 1 cut(s) 1093
Bme18I GGWCC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 3 cut(s) 304, 916, 946
BmrFI CCNGG 1 cut(s) 1093
BmsI GCATC 3 cut(s) 350, 727, 737
BplI GAGNNNNNCTC 2 cut(s) 810, 842
Bpu14I TTCGAA 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 2 cut(s) 898, 1092
BsaWI WCCGGW 1 cut(s) 380
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 424
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 686
Bse21I CCTNAGG 1 cut(s) 603
BseBI CCWGG 1 cut(s) 1093
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 898, 1092
BseGI GGATG 2 cut(s) 752, 1057
BseLI CCNNNNNNNGG 2 cut(s) 231, 424
BseMII CTCAG 2 cut(s) 269, 617
BseNI ACTGG 1 cut(s) 686
BseRI GAGGAG 2 cut(s) 597, 847
BseXI GCAGC 3 cut(s) 170, 578, 1060
BseYI CCCAGC 1 cut(s) 1047
BshNI GGYRCC 2 cut(s) 914, 944
BshVI ATCGAT 1 cut(s) 296
BsiHKAI GWGCWC 3 cut(s) 80, 879, 1004
BsiSI CCGG 2 cut(s) 144, 381
BslI CCNNNNNNNGG 2 cut(s) 231, 424
BsmAI GTCTC 2 cut(s) 616, 794
BsmBI CGTCTC 1 cut(s) 794
BsmI GAATGC 1 cut(s) 428
Bso31I GGTCTC 1 cut(s) 616
Bsp119I TTCGAA 1 cut(s) 557
Bsp1286I GDGCHC 4 cut(s) 80, 879, 906, 1004
Bsp143I GATC 5 cut(s) 8, 111, 302, 990, 1027
Bsp19I CCATGG 1 cut(s) 898
BspACI CCGC 2 cut(s) 147, 168
BspCNI CTCAG 2 cut(s) 270, 616
BspDI ATCGAT 1 cut(s) 296
BspLI GGNNCC 3 cut(s) 304, 916, 946
BspPI GGATC 4 cut(s) 16, 297, 310, 1022
BspT104I TTCGAA 1 cut(s) 557
BspT107I GGYRCC 2 cut(s) 914, 944
BspTI CTTAAG 1 cut(s) 794
BspTNI GGTCTC 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 1 cut(s) 686
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 2 cut(s) 898, 1092
BssMI GATC 5 cut(s) 8, 111, 302, 990, 1027
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 1 cut(s) 1093
Bst4CI ACNGT 6 cut(s) 174, 183, 349, 631, 962, 1006
Bst6I CTCTTC 2 cut(s) 441, 1140
BstAFI CTTAAG 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 557
BstC8I GCNNGC 2 cut(s) 660, 807
BstDEI CTNAG 2 cut(s) 278, 603
BstDSI CCRYGG 1 cut(s) 898
BstF5I GGATG 2 cut(s) 752, 1057
BstKTI GATC 5 cut(s) 11, 114, 305, 993, 1030
BstMAI GTCTC 2 cut(s) 616, 794
BstMBI GATC 5 cut(s) 8, 111, 302, 990, 1027
BstMWI GCNNNNNNNGC 3 cut(s) 167, 338, 1070
BstNI CCWGG 1 cut(s) 1093
BstNSI RCATGY 2 cut(s) 529, 793
BstSCI CCNGG 1 cut(s) 1091
BstSFI CTRYAG 1 cut(s) 1161
BstV1I GCAGC 3 cut(s) 170, 578, 1060
BstX2I RGATCY 2 cut(s) 8, 302
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 2 cut(s) 8, 302
Bsu15I ATCGAT 1 cut(s) 296
Bsu36I CCTNAGG 1 cut(s) 603
BsuTUI ATCGAT 1 cut(s) 296
BtgI CCRYGG 1 cut(s) 898
BtsCI GGATG 2 cut(s) 752, 1057
BtsIMutI CAGTG 3 cut(s) 91, 1002, 1065
Cac8I GCNNGC 2 cut(s) 660, 807
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 179
ClaI ATCGAT 1 cut(s) 296
CseI GACGC 1 cut(s) 811
Csp6I GTAC 4 cut(s) 627, 671, 773, 915
CviAII CATG 8 cut(s) 190, 317, 526, 790, 899, 924, 935, 1061
CviQI GTAC 4 cut(s) 627, 671, 773, 915
DdeI CTNAG 2 cut(s) 278, 603
DpnI GATC 5 cut(s) 10, 113, 304, 992, 1029
DpnII GATC 5 cut(s) 8, 111, 302, 990, 1027
Eam1104I CTCTTC 2 cut(s) 441, 1140
EarI CTCTTC 2 cut(s) 441, 1140
Ecl136II GAGCTC 2 cut(s) 78, 877
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 3 cut(s) 80, 879, 906
Eco31I GGTCTC 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 179
Eco53kI GAGCTC 2 cut(s) 78, 877
Eco57I CTGAAG 1 cut(s) 1089
Eco81I CCTNAGG 1 cut(s) 603
EcoICRI GAGCTC 2 cut(s) 78, 877
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 1091
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 3 cut(s) 80, 879, 906
ErhI CCWWGG 1 cut(s) 898
Esp3I CGTCTC 1 cut(s) 794
FaeI CATG 8 cut(s) 193, 320, 529, 793, 902, 927, 938, 1064
FatI CATG 8 cut(s) 189, 316, 525, 789, 898, 923, 934, 1060
FauI CCCGC 2 cut(s) 140, 175
FbaI TGATCA 1 cut(s) 990
Fnu4HI GCNGC 3 cut(s) 159, 567, 1074
FokI GGATG 2 cut(s) 759, 1064
FriOI GRGCYC 3 cut(s) 80, 879, 906
Fsp4HI GCNGC 3 cut(s) 159, 567, 1074
FspBI CTAG 4 cut(s) 263, 401, 680, 872
GluI GCNGC 3 cut(s) 159, 567, 1074
GsaI CCCAGC 1 cut(s) 1051
HapII CCGG 2 cut(s) 144, 381
HgaI GACGC 1 cut(s) 811
Hin1II CATG 8 cut(s) 193, 320, 529, 793, 902, 927, 938, 1064
HindIII AAGCTT 2 cut(s) 38, 1082
HinfI GANTC 6 cut(s) 89, 128, 280, 384, 854, 1056
HpaII CCGG 2 cut(s) 144, 381
HphI GGTGA 4 cut(s) 23, 137, 1000, 1130
Hpy166II GTNNAC 3 cut(s) 31, 325, 853
Hpy188I TCNGA 4 cut(s) 139, 226, 307, 730
Hpy188III TCNNGA 4 cut(s) 177, 202, 277, 433
Hpy8I GTNNAC 3 cut(s) 31, 325, 853
HpyAV CCTTC 2 cut(s) 418, 504
HpyCH4III ACNGT 6 cut(s) 174, 183, 349, 631, 962, 1006
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 5 cut(s) 662, 770, 809, 1013, 1076
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 338, 1070
HpyF3I CTNAG 2 cut(s) 278, 603
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 8 cut(s) 193, 320, 529, 793, 902, 927, 938, 1064
KpnI GGTACC 1 cut(s) 918
Ksp22I TGATCA 1 cut(s) 990
Kzo9I GATC 5 cut(s) 8, 111, 302, 990, 1027
LmnI GCTCC 2 cut(s) 637, 999
Lsp1109I GCAGC 3 cut(s) 170, 578, 1060
LweI GCATC 3 cut(s) 350, 727, 737
MaeI CTAG 4 cut(s) 263, 401, 680, 872
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 5 cut(s) 125, 376, 1006, 1087, 1148
MalI GATC 5 cut(s) 10, 113, 304, 992, 1029
MboI GATC 5 cut(s) 8, 111, 302, 990, 1027
MboII GAAGA 5 cut(s) 3, 106, 458, 590, 1127
MflI RGATCY 2 cut(s) 8, 302
MhlI GDGCHC 4 cut(s) 80, 879, 906, 1004
MluCI AATT 5 cut(s) 133, 559, 618, 952, 1036
MlyI GAGTC 3 cut(s) 122, 289, 848
MmeI TCCRAC 1 cut(s) 486
MroXI GAANNNNTTC 2 cut(s) 428, 450
MseI TTAA 2 cut(s) 675, 795
MslI CAYNNNNRTG 1 cut(s) 939
MspCI CTTAAG 1 cut(s) 794
MspI CCGG 2 cut(s) 144, 381
MspR9I CCNGG 1 cut(s) 1093
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 1 cut(s) 1093
MwoI GCNNNNNNNGC 3 cut(s) 167, 338, 1070
NcoI CCATGG 1 cut(s) 898
NdeII GATC 5 cut(s) 8, 111, 302, 990, 1027
NlaIII CATG 8 cut(s) 193, 320, 529, 793, 902, 927, 938, 1064
NlaIV GGNNCC 3 cut(s) 304, 916, 946
NmuCI GTSAC 3 cut(s) 125, 1006, 1148
NspI RCATGY 2 cut(s) 529, 793
NspV TTCGAA 1 cut(s) 557
PciI ACATGT 1 cut(s) 525
PctI GAATGC 1 cut(s) 428
PdmI GAANNNNTTC 2 cut(s) 428, 450
PfeI GAWTC 3 cut(s) 89, 384, 1056
PkrI GCNGC 3 cut(s) 160, 568, 1075
PleI GAGTC 3 cut(s) 122, 288, 848
PpsI GAGTC 3 cut(s) 122, 288, 848
PscI ACATGT 1 cut(s) 525
Psp124BI GAGCTC 2 cut(s) 80, 879
Psp1406I AACGTT 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 1091
PspFI CCCAGC 1 cut(s) 1047
PspGI CCWGG 1 cut(s) 1091
PspN4I GGNNCC 3 cut(s) 304, 916, 946
PspPI GGNCC 1 cut(s) 179
PsuI RGATCY 2 cut(s) 8, 302
RsaI GTAC 4 cut(s) 628, 672, 774, 916
RsaNI GTAC 4 cut(s) 627, 671, 773, 915
RseI CAYNNNNRTG 1 cut(s) 939
SacI GAGCTC 2 cut(s) 80, 879
SaqAI TTAA 2 cut(s) 675, 795
SatI GCNGC 3 cut(s) 159, 567, 1074
Sau3AI GATC 5 cut(s) 8, 111, 302, 990, 1027
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 3 cut(s) 122, 289, 848
ScrFI CCNGG 1 cut(s) 1093
SduI GDGCHC 4 cut(s) 80, 879, 906, 1004
SfaNI GCATC 3 cut(s) 350, 727, 737
SfcI CTRYAG 1 cut(s) 1161
SfuI TTCGAA 1 cut(s) 557
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 939
SmlI CTYRAG 2 cut(s) 255, 794
SmoI CTYRAG 2 cut(s) 255, 794
Sse9I AATT 5 cut(s) 133, 559, 618, 952, 1036
SsiI CCGC 2 cut(s) 147, 168
SspMI CTAG 4 cut(s) 263, 401, 680, 872
SstI GAGCTC 2 cut(s) 80, 879
StyD4I CCNGG 1 cut(s) 1091
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 6 cut(s) 174, 183, 349, 631, 962, 1006
TaiI ACGT 1 cut(s) 74
TaqI TCGA 7 cut(s) 105, 131, 296, 557, 735, 752, 1119
TasI AATT 5 cut(s) 133, 559, 618, 952, 1036
TatI WGTACW 1 cut(s) 772
TfiI GAWTC 3 cut(s) 89, 384, 1056
Tru1I TTAA 2 cut(s) 675, 795
Tru9I TTAA 2 cut(s) 675, 795
TscAI CASTG 3 cut(s) 91, 1009, 1072
TseFI GTSAC 3 cut(s) 125, 1006, 1148
TseI GCWGC 3 cut(s) 158, 566, 1073
Tsp45I GTSAC 3 cut(s) 125, 1006, 1148
TspDTI ATGAA 2 cut(s) 437, 443
TspRI CASTG 3 cut(s) 91, 1009, 1072
Vha464I CTTAAG 1 cut(s) 794
VpaK11BI GGWCC 1 cut(s) 179
XapI RAATTY 2 cut(s) 133, 559
XceI RCATGY 2 cut(s) 529, 793
XmnI GAANNNNTTC 2 cut(s) 428, 450
XspI CTAG 4 cut(s) 263, 401, 680, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.