RchiOBHm_Chr1g0358891

Enoyl-CoA hydratase/isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0017357
Physical Location & Seq
Reverse (-)
50871585 .. 50872772
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGGCTTCTTTCAACTCGGATCGTCGTCCTGATCCAGAGGTTCTGGTACAAGAGAACAATTCATCTACGAGGACATTGGCACTAAACAGGCCTCGACACATGAATGCTCTCTCTCTTGAAATGTTCTCTCGGCTATCAGACCTGTTCCTCGCCTATGATCATGATACCAAAGTCAAACTCGTCATGCTCAAAGGTACTGGAAAAGCATTTTCTGCAGGCGGTGATATCGCTGCTATTGCTCGTCATCTCACCAATGGTGATTGGAGGTCTGTTGTCAAAGAGTCCGATGAGGCCTATAAATTACTGTTCTTGATAGCAACATACAGTAAACCCCAGCTTTCAGTTCTCAATGGCATAGCCATAGGAAGGGGAGCCTGTATTTTTTTACCCTCTAAATTCCGTATAGTAACGGAGAATTCAACATTTTCTATTCCAGAGACAGCTTTAGGAGGGTTTCCTGACATGGGTGCCTCTTATTTCCTGTCGAGATTTCCAGGATTTTTTGGAGAGTATCTTGTCCTAACTGGTGCCAAATTGGAAGGACCTGAAATGCTTGCTTGCGGTCTAGCAACTCACTTTGTTCCGGCAACCAAATTGACTTTGCTAGAAGAAGCACTAGCATGCAAAGTTGCTTCGACAAACGATAGCACCTCAATCACTGATCTTCTATTTTATATTTCAGCTGTTATAAATGAATATTCAGTTCAACCAGCTTTGAAAGAAACAGGTAGCGCTTGTCACAAAATGGATGTTATCGAAAAGTGCTTTTCGAACAGAACAGTGGAAGAGATTTTAAGTGCACTCGAGAAGGAGGCTGATACATATCTTGAGGATGATCCATGGATGTTATCCACAATTCAATCACTTAAGAAGGCGTCGCCAACAGGTTTGAAGATTTCTTTGAGATCGATTAGAGAAGGAAGATCGCAAGGACTCGGTGAGTGTCTTGTTCGAGAACATAGAATGCTGTATCATCTTGTAAGGGGAAAAGTAAGCAGAGATTTCATAGAGGGTTGCACAACACTACTGTGGGATAAGAATAAGATCCATAAGTGGAACCCTTGTAAGTTGGAGCTCGTCACTGATCAGATGATCGATCTCTTCTTCTCCAAGTTGGATGATGATGCAGAGCCCGAAGAATTAAAGCTCCCTGAAAGATCCAACTTGCGTACCATTGCCAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200
Pfam Domains
Protein Families
ECH_2

Protein Analysis

395

Amino Acids

44.03

Weight (kDa)

6.12

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 20 - 208 2e-27 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 24 - 369 3.3e-98 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06730 FvH4_7g06750 FvH4_7g16010
rosa_chinensis RchiOBHm_Chr1g0333381 RchiOBHm_Chr1g0358891
rosa_laevigata RLG00000027917 RLG00000029609
rosa_multiflora Rmu_co8464851.1_g000001
rosa_rugosa Rorug01G0109000
rosa_samantha Rh1AG131800 Rh1BG101200 Rh1BG101700
rosa_wichuraiana Rw1G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AccB1I GGYRCC 2 cut(s) 467, 527
AciI CCGC 2 cut(s) 219, 561
AclWI GGATC 5 cut(s) 26, 27, 830, 1039, 1152
AcsI RAATTY 2 cut(s) 395, 415
AcyI GRCGYC 1 cut(s) 875
AfaI GTAC 3 cut(s) 48, 196, 1171
AfeI AGCGCT 1 cut(s) 733
AfiI CCNNNNNNNGG 2 cut(s) 366, 464
AflII CTTAAG 1 cut(s) 866
AgsI TTSAA 7 cut(s) 13, 119, 420, 707, 718, 860, 892
AjnI CCWGG 1 cut(s) 493
AleI CACNNNNGTG 1 cut(s) 1027
AluBI AGCT 6 cut(s) 337, 443, 683, 713, 1075, 1147
AluI AGCT 6 cut(s) 337, 443, 683, 713, 1075, 1147
Alw21I GWGCWC 2 cut(s) 802, 1077
Alw26I GTCTC 1 cut(s) 431
Alw44I GTGCAC 1 cut(s) 798
AlwI GGATC 5 cut(s) 26, 27, 830, 1039, 1152
Ama87I CYCGRG 1 cut(s) 803
Aor51HI AGCGCT 1 cut(s) 733
AoxI GGCC 2 cut(s) 89, 291
ApaLI GTGCAC 1 cut(s) 798
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 2 cut(s) 395, 415
AspLEI GCGC 1 cut(s) 734
AspS9I GGNCC 1 cut(s) 542
AsuHPI GGTGA 4 cut(s) 233, 241, 269, 950
AsuII TTCGAA 1 cut(s) 770
AvaI CYCGRG 1 cut(s) 803
AvaII GGWCC 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 802
BaeI ACNNNNGTAYC 4 cut(s) 156, 189, 1153, 1186
BanI GGYRCC 2 cut(s) 467, 527
BanII GRGCYC 2 cut(s) 1077, 1134
Bbv12I GWGCWC 2 cut(s) 802, 1077
BbvI GCAGC 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 495
BclI TGATCA 2 cut(s) 157, 1084
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 3 cut(s) 566, 605, 617
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 735
BfrI CTTAAG 1 cut(s) 866
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 803
BmgT120I GGNCC 1 cut(s) 542
BmiI GGNNCC 4 cut(s) 373, 469, 529, 1058
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 1114
Bpu14I TTCGAA 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 848
Bsa29I ATCGAT 2 cut(s) 908, 1095
BsaBI GATNNNNATC 1 cut(s) 822
BsaHI GRCGYC 1 cut(s) 875
BsaJI CCNNGG 1 cut(s) 839
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 464
Bse1I ACTGG 2 cut(s) 202, 529
Bse3DI GCAATG 1 cut(s) 1173
Bse8I GATNNNNATC 1 cut(s) 822
BseBI CCWGG 1 cut(s) 495
BseCI ATCGAT 2 cut(s) 908, 1095
BseDI CCNNGG 1 cut(s) 839
BseGI GGATG 4 cut(s) 754, 838, 849, 1123
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 2 cut(s) 366, 464
BseMI GCAATG 1 cut(s) 1173
BseNI ACTGG 2 cut(s) 202, 529
BseSI GKGCMC 1 cut(s) 802
BseXI GCAGC 1 cut(s) 217
BseYI CCCAGC 1 cut(s) 333
BshFI GGCC 2 cut(s) 91, 293
BshNI GGYRCC 2 cut(s) 467, 527
BshVI ATCGAT 2 cut(s) 908, 1095
BsiHKAI GWGCWC 2 cut(s) 802, 1077
BsiHKCI CYCGRG 1 cut(s) 803
BsiSI CCGG 1 cut(s) 584
BslI CCNNNNNNNGG 2 cut(s) 366, 464
BsmAI GTCTC 1 cut(s) 431
BsmI GAATGC 2 cut(s) 109, 969
BsnI GGCC 2 cut(s) 91, 293
BsoBI CYCGRG 1 cut(s) 803
Bsp119I TTCGAA 1 cut(s) 770
Bsp1286I GDGCHC 3 cut(s) 802, 1077, 1134
Bsp19I CCATGG 1 cut(s) 839
BspACI CCGC 2 cut(s) 219, 561
BspANI GGCC 2 cut(s) 91, 293
BspDI ATCGAT 2 cut(s) 908, 1095
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 4 cut(s) 373, 469, 529, 1058
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 5 cut(s) 26, 27, 830, 1039, 1152
BspT104I TTCGAA 1 cut(s) 770
BspT107I GGYRCC 2 cut(s) 467, 527
BspTI CTTAAG 1 cut(s) 866
BsrDI GCAATG 1 cut(s) 1173
BsrI ACTGG 2 cut(s) 202, 529
BssECI CCNNGG 1 cut(s) 839
BssNI GRCGYC 1 cut(s) 875
BssT1I CCWWGG 1 cut(s) 839
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 4 cut(s) 306, 326, 781, 1029
Bst6I CTCTTC 2 cut(s) 780, 1106
BstACI GRCGYC 1 cut(s) 875
BstAFI CTTAAG 1 cut(s) 866
BstAPI GCANNNNNTGC 1 cut(s) 212
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 4 cut(s) 217, 555, 559, 622
BstDSI CCRYGG 1 cut(s) 839
BstF5I GGATG 4 cut(s) 754, 838, 849, 1123
BstH2I RGCGCY 1 cut(s) 735
BstHHI GCGC 1 cut(s) 734
BstMAI GTCTC 1 cut(s) 431
BstMWI GCNNNNNNNGC 2 cut(s) 212, 236
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 802
BstV1I GCAGC 1 cut(s) 217
BstX2I RGATCY 2 cut(s) 1044, 1157
BstYI RGATCY 2 cut(s) 1044, 1157
Bsu15I ATCGAT 2 cut(s) 908, 1095
BsuRI GGCC 2 cut(s) 91, 293
BsuTUI ATCGAT 2 cut(s) 908, 1095
BtgI CCRYGG 1 cut(s) 839
BtsCI GGATG 4 cut(s) 754, 838, 849, 1123
BtsIMutI CAGTG 3 cut(s) 657, 786, 1080
Cac8I GCNNGC 4 cut(s) 217, 555, 559, 622
CciI TCATGA 1 cut(s) 160
CfoI GCGC 1 cut(s) 734
Cfr13I GGNCC 1 cut(s) 542
ClaI ATCGAT 2 cut(s) 908, 1095
CseI GACGC 1 cut(s) 864
Csp6I GTAC 3 cut(s) 47, 195, 1170
CviAII CATG 6 cut(s) 100, 161, 184, 463, 621, 840
CviQI GTAC 3 cut(s) 47, 195, 1170
Eam1104I CTCTTC 2 cut(s) 780, 1106
EarI CTCTTC 2 cut(s) 780, 1106
Ecl136II GAGCTC 1 cut(s) 1075
Eco130I CCWWGG 1 cut(s) 839
Eco147I AGGCCT 2 cut(s) 91, 293
Eco24I GRGCYC 2 cut(s) 1077, 1134
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 542
Eco47III AGCGCT 1 cut(s) 733
Eco53kI GAGCTC 1 cut(s) 1075
Eco88I CYCGRG 1 cut(s) 803
EcoICRI GAGCTC 1 cut(s) 1075
EcoO109I RGGNCCY 1 cut(s) 542
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 493
EcoRV GATATC 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 839
EcoT38I GRGCYC 2 cut(s) 1077, 1134
ErhI CCWWGG 1 cut(s) 839
FaeI CATG 6 cut(s) 103, 164, 187, 466, 624, 843
FatI CATG 6 cut(s) 99, 160, 183, 462, 620, 839
FbaI TGATCA 2 cut(s) 157, 1084
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 4 cut(s) 761, 845, 856, 1130
FriOI GRGCYC 2 cut(s) 1077, 1134
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 3 cut(s) 566, 605, 617
GlaI GCGC 1 cut(s) 733
GluI GCNGC 1 cut(s) 231
GsaI CCCAGC 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 735
HaeIII GGCC 2 cut(s) 91, 293
HapII CCGG 1 cut(s) 584
HgaI GACGC 1 cut(s) 864
HhaI GCGC 1 cut(s) 734
Hin1I GRCGYC 1 cut(s) 875
Hin1II CATG 6 cut(s) 103, 164, 187, 466, 624, 843
Hin6I GCGC 1 cut(s) 732
HinP1I GCGC 1 cut(s) 732
HinfI GANTC 2 cut(s) 281, 933
HpaII CCGG 1 cut(s) 584
HphI GGTGA 4 cut(s) 233, 241, 269, 950
Hpy166II GTNNAC 2 cut(s) 329, 800
Hpy188I TCNGA 4 cut(s) 19, 139, 286, 1089
Hpy8I GTNNAC 2 cut(s) 329, 800
Hpy99I CGWCG 2 cut(s) 27, 880
HpyAV CCTTC 5 cut(s) 360, 533, 802, 865, 911
HpyCH4III ACNGT 4 cut(s) 306, 326, 781, 1029
HpyCH4V TGCA 5 cut(s) 215, 624, 800, 1017, 1127
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 236
Hsp92I GRCGYC 1 cut(s) 875
Hsp92II CATG 6 cut(s) 103, 164, 187, 466, 624, 843
HspAI GCGC 1 cut(s) 732
Ksp22I TGATCA 2 cut(s) 157, 1084
LmnI GCTCC 3 cut(s) 371, 1072, 1152
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 1114
MaeI CTAG 3 cut(s) 566, 605, 617
MaeIII GTNAC 3 cut(s) 406, 737, 1078
MboII GAAGA 8 cut(s) 620, 656, 797, 904, 933, 1093, 1096, 1148
MflI RGATCY 2 cut(s) 1044, 1157
MhlI GDGCHC 3 cut(s) 802, 1077, 1134
MluCI AATT 8 cut(s) 58, 299, 395, 415, 533, 593, 855, 1139
MlyI GAGTC 2 cut(s) 290, 927
MmeI TCCRAC 3 cut(s) 1050, 1095, 1185
MseI TTAA 3 cut(s) 794, 867, 1142
MslI CAYNNNNRTG 3 cut(s) 102, 619, 1027
MspA1I CMGCKG 1 cut(s) 683
MspCI CTTAAG 1 cut(s) 866
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 1 cut(s) 495
Mva1269I GAATGC 2 cut(s) 109, 969
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 2 cut(s) 212, 236
NcoI CCATGG 1 cut(s) 839
NlaIII CATG 6 cut(s) 103, 164, 187, 466, 624, 843
NlaIV GGNNCC 4 cut(s) 373, 469, 529, 1058
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 2 cut(s) 737, 1078
NspI RCATGY 1 cut(s) 624
NspV TTCGAA 1 cut(s) 770
OliI CACNNNNGTG 1 cut(s) 1027
PaeI GCATGC 1 cut(s) 624
PaeR7I CTCGAG 1 cut(s) 803
PagI TCATGA 1 cut(s) 160
PceI AGGCCT 2 cut(s) 91, 293
PctI GAATGC 2 cut(s) 109, 969
PfoI TCCNGGA 1 cut(s) 493
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 2 cut(s) 289, 927
PpsI GAGTC 2 cut(s) 289, 927
PpuMI RGGWCCY 1 cut(s) 542
PsiI TTATAA 1 cut(s) 689
Psp124BI GAGCTC 1 cut(s) 1077
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 493
PspFI CCCAGC 1 cut(s) 333
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 4 cut(s) 373, 469, 529, 1058
PspPI GGNCC 1 cut(s) 542
PspPPI RGGWCCY 1 cut(s) 542
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 2 cut(s) 1044, 1157
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 3 cut(s) 48, 196, 1171
RsaNI GTAC 3 cut(s) 47, 195, 1170
RseI CAYNNNNRTG 3 cut(s) 102, 619, 1027
SacI GAGCTC 1 cut(s) 1077
SaqAI TTAA 3 cut(s) 794, 867, 1142
SatI GCNGC 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 542
SchI GAGTC 2 cut(s) 290, 927
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 3 cut(s) 802, 1077, 1134
SfaNI GCATC 1 cut(s) 1114
SfcI CTRYAG 1 cut(s) 213
Sfr274I CTCGAG 1 cut(s) 803
SfuI TTCGAA 1 cut(s) 770
SinI GGWCC 1 cut(s) 542
SlaI CTCGAG 1 cut(s) 803
SmiMI CAYNNNNRTG 3 cut(s) 102, 619, 1027
SmlI CTYRAG 3 cut(s) 803, 827, 866
SmoI CTYRAG 3 cut(s) 803, 827, 866
SphI GCATGC 1 cut(s) 624
Sse9I AATT 8 cut(s) 58, 299, 395, 415, 533, 593, 855, 1139
SseBI AGGCCT 2 cut(s) 91, 293
SsiI CCGC 2 cut(s) 219, 561
SspI AATATT 1 cut(s) 698
SspMI CTAG 3 cut(s) 566, 605, 617
SstI GAGCTC 1 cut(s) 1077
StuI AGGCCT 2 cut(s) 91, 293
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 1 cut(s) 839
TaaI ACNGT 4 cut(s) 306, 326, 781, 1029
TaqI TCGA 9 cut(s) 94, 485, 635, 756, 770, 804, 908, 952, 1095
TasI AATT 8 cut(s) 58, 299, 395, 415, 533, 593, 855, 1139
Tru1I TTAA 3 cut(s) 794, 867, 1142
Tru9I TTAA 3 cut(s) 794, 867, 1142
TscAI CASTG 3 cut(s) 664, 786, 1087
TseFI GTSAC 2 cut(s) 737, 1078
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 2 cut(s) 737, 1078
TspDTI ATGAA 4 cut(s) 51, 116, 708, 994
TspGWI ACGGA 2 cut(s) 389, 425
TspRI CASTG 3 cut(s) 664, 786, 1087
Vha464I CTTAAG 1 cut(s) 866
VneI GTGCAC 1 cut(s) 798
VpaK11BI GGWCC 1 cut(s) 542
XapI RAATTY 2 cut(s) 395, 415
XceI RCATGY 1 cut(s) 624
XhoI CTCGAG 1 cut(s) 803
XspI CTAG 3 cut(s) 566, 605, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.